FastQCFastQC Report
Thu 26 May 2016
SRR938889_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938889_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3144866
Sequences flagged as poor quality0
Sequence length101
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT128040.4071397636656061No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT107520.34189056067889695No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT71020.22582838187700208No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT61450.1953978325308614No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA199450.023.5265031
GGTATCA151050.022.1081541
GTACATG98900.022.0609571
TACATGG96600.021.6167892
GAGTACT109600.020.67755512-13
ACATGGG98600.020.2156643
AGTACTT115850.019.93072712-13
ATCAACG233500.019.7761443
TCAACGC235150.019.6979264
TATCAAC236000.019.687312
CAACGCA235300.019.6651975
AGAGTAC174000.019.47535110-11
AACGCAG239500.019.3797866
GTACTTT118350.019.0084414-15
ACGCAGA260550.017.4133557
CGCAGAG262300.017.080068
CATGGGG51500.016.8639584
GTATAGG9400.016.7240431
TACTTTT137000.016.69793714-15
GCAGAGT267400.016.2223689
Produced by FastQC (version 0.11.5)