##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938889_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 3144866 Sequences flagged as poor quality 0 Sequence length 101 %GC 44 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.045567919269054 33.0 31.0 34.0 30.0 34.0 2 32.306390796936974 34.0 31.0 34.0 30.0 34.0 3 32.389954611738624 34.0 31.0 34.0 30.0 34.0 4 35.976159874538375 37.0 35.0 37.0 35.0 37.0 5 35.8973501573676 37.0 35.0 37.0 35.0 37.0 6 35.93592699975134 37.0 35.0 37.0 35.0 37.0 7 35.871462885859046 37.0 35.0 37.0 35.0 37.0 8 35.83337573047628 37.0 35.0 37.0 33.0 37.0 9 37.49007620674458 39.0 37.0 39.0 35.0 39.0 10-11 37.38224410833403 39.0 37.0 39.0 34.0 39.0 12-13 37.275221742357225 39.0 37.0 39.0 34.0 39.0 14-15 38.54351489061855 40.0 38.0 41.0 34.0 41.0 16-17 38.645885230086115 40.0 38.0 41.0 34.0 41.0 18-19 38.673573214248236 40.0 38.0 41.0 34.0 41.0 20-21 38.64207886758927 40.0 38.0 41.0 34.0 41.0 22-23 38.56030272831974 40.0 38.0 41.0 34.0 41.0 24-25 38.473579160447535 40.0 38.0 41.0 34.0 41.0 26-27 38.29216586652659 40.0 38.0 41.0 34.0 41.0 28-29 38.11080217726288 40.0 38.0 41.0 33.0 41.0 30-31 37.89997634239424 40.0 37.0 41.0 33.0 41.0 32-33 37.786155117578936 40.0 37.0 41.0 33.0 41.0 34-35 37.6729614234756 40.0 37.0 41.0 33.0 41.0 36-37 37.529428757854866 40.0 37.0 41.0 32.0 41.0 38-39 37.37454552912588 40.0 36.5 41.0 31.5 41.0 40-41 37.18546815667186 40.0 36.0 41.0 31.0 41.0 42-43 36.980385491782485 39.5 36.0 41.0 31.0 41.0 44-45 36.70722822530435 39.0 36.0 41.0 30.0 41.0 46-47 36.37502202001612 39.0 35.0 41.0 30.0 41.0 48-49 36.01698482542658 39.0 35.0 40.0 29.0 41.0 50-51 35.724464253802864 39.0 35.0 40.0 28.0 41.0 52-53 35.433469025389314 38.0 34.5 40.0 27.5 41.0 54-55 35.09366774291814 38.0 34.0 40.0 27.0 41.0 56-57 34.9136586423714 38.0 34.0 40.0 26.0 41.0 58-59 34.5797051448297 38.0 34.0 40.0 26.0 41.0 60-61 34.15784027045986 37.0 33.0 40.0 25.5 41.0 62-63 33.6845822683701 37.0 33.0 39.5 23.5 41.0 64-65 33.221174765474906 36.0 32.0 39.0 22.5 40.0 66-67 32.69990470182195 35.5 31.5 39.0 21.5 40.0 68-69 32.151334587864795 35.0 31.0 38.0 20.0 40.0 70-71 31.55201064210685 35.0 30.5 37.5 18.0 39.5 72-73 30.960585284078874 34.0 30.0 37.0 15.0 39.0 74-75 30.363485757421778 34.0 29.0 36.0 10.0 39.0 76-77 28.539534116874933 31.5 27.0 34.5 7.5 36.5 78-79 29.498324888882387 33.5 29.0 35.0 7.0 37.0 80-81 29.433391756596308 34.0 29.0 35.0 4.5 37.0 82-83 28.921600634176464 34.0 29.0 35.0 2.0 36.5 84-85 28.35774799307824 33.0 27.5 35.0 2.0 36.0 86-87 27.528501373349453 33.0 26.0 35.0 2.0 35.5 88-89 26.7015554875788 32.0 25.0 34.5 2.0 35.0 90-91 25.961675950581043 31.5 23.5 34.0 2.0 35.0 92-93 25.384219391223667 31.0 21.5 34.0 2.0 35.0 94-95 24.61719704432558 31.0 19.0 34.0 2.0 35.0 96-97 23.495450203601678 31.0 4.5 34.0 2.0 35.0 98-99 21.95343187913253 30.0 2.0 34.0 2.0 35.0 100-101 16.423225027711833 14.5 2.0 29.5 2.0 32.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.11032742549027574 1101 2 0.1022690686923795 1101 3 0.08853855335481597 1101 4 0.08226258013115739 1101 5 0.09390438494354925 1101 6 0.1216348425022531 1101 7 0.18069626686870066 1101 8 0.1578469586541047 1101 9 0.13658558539076182 1101 10-11 0.28993668734074163 1101 12-13 0.286671543114025 1101 14-15 0.2367705198226986 1101 16-17 0.25098683948118605 1101 18-19 0.2807693440160577 1101 20-21 0.3647930180168544 1101 22-23 0.3105743147994602 1101 24-25 0.2467243174101057 1101 26-27 0.1946979121667951 1101 28-29 0.2702094242016173 1101 30-31 0.25792326511830765 1101 32-33 0.3411533460183591 1101 34-35 0.4031283566123207 1101 36-37 0.5162771565713484 1101 38-39 0.41057876506721414 1101 40-41 0.45147416305464816 1101 42-43 0.5238619192112139 1101 44-45 0.5263056559155217 1101 46-47 0.6466252305930595 1101 48-49 0.5371468705160325 1101 50-51 0.3765898191164041 1101 52-53 0.29525281293786776 1101 54-55 0.4355351915654424 1101 56-57 0.3554100183690281 1101 58-59 0.4228001059267825 1101 60-61 0.6227950422526973 1101 62-63 0.5448282139336982 1101 64-65 0.5347249487219159 1101 66-67 0.5395218137929518 1101 68-69 0.5845141052681271 1101 70-71 0.6270409444532135 1101 72-73 0.7629221499126011 1101 74-75 0.7771161074076964 1101 76-77 0.42468750829527835 1101 78-79 0.9129472893547188 1101 80-81 0.9263685669365849 1101 82-83 0.8387135376753427 1101 84-85 0.82732245772592 1101 86-87 0.9473093482945316 1101 88-89 0.9588048594789882 1101 90-91 0.9039466260183495 1101 92-93 0.8622299047841224 1101 94-95 0.815417680841346 1101 96-97 1.0133757806561121 1101 98-99 1.243595211610529 1101 100-101 1.6743791605612586 1102 1 0.21784960699172018 1102 2 0.16056786603964923 1102 3 0.23799229332175997 1102 4 0.10301175508903526 1102 5 0.10182872119362685 1102 6 0.10433779504069207 1102 7 0.15481549139831685 1102 8 0.18321297925593427 1102 9 0.1868132029323064 1102 10-11 0.25816396465459235 1102 12-13 0.2441828016214771 1102 14-15 0.3106532901186583 1102 16-17 0.28058524442315047 1102 18-19 0.2900467172496519 1102 20-21 0.2833411344573733 1102 22-23 0.30221586727285654 1102 24-25 0.3057280440668464 1102 26-27 0.30263745434790934 1102 28-29 0.3031063927849189 1102 30-31 0.22961360295276734 1102 32-33 0.3024820856091708 1102 34-35 0.29394671253822935 1102 36-37 0.1849211125046395 1102 38-39 0.19817949182245087 1102 40-41 0.2823132957388381 1102 42-43 0.341436635398928 1102 44-45 0.3759366513001865 1102 46-47 0.386859281567439 1102 48-49 0.47767728669113296 1102 50-51 0.4746661183859828 1102 52-53 0.4516445096970969 1102 54-55 0.2973861485661544 1102 56-57 0.4053507192501087 1102 58-59 0.3553117506006842 1102 60-61 0.32701977347583266 1102 62-63 0.5003571847429242 1102 64-65 0.4554126780772094 1102 66-67 0.22683787067436612 1102 68-69 0.38944417704293244 1102 70-71 0.5078073374198127 1102 72-73 0.538530352047971 1102 74-75 0.6322642177899205 1102 76-77 0.4129314479393642 1102 78-79 0.5568959005296357 1102 80-81 0.7187569051509968 1102 82-83 0.8255362343128105 1102 84-85 0.9866022783521018 1102 86-87 1.0162339419220423 1102 88-89 0.7131514387563556 1102 90-91 0.8699352913809406 1102 92-93 0.8851729565663362 1102 94-95 1.052974143893639 1102 96-97 1.1399520168351174 1102 98-99 1.3072691450940077 1102 100-101 1.0694784976187748 1103 1 0.09883927896056832 1103 2 0.11543843931185194 1103 3 0.060439272658634025 1103 4 0.0345816638413865 1103 5 -5.96755114209202E-4 1103 6 -0.00463159895439702 1103 7 0.0769599192024728 1103 8 0.013402602561519927 1103 9 0.10011098437574617 1103 10-11 0.13302104658177427 1103 12-13 0.06610562192793168 1103 14-15 0.1359784028844757 1103 16-17 0.2159366438248682 1103 18-19 0.22568980368686198 1103 20-21 0.24588086250398078 1103 22-23 0.21314575323498275 1103 24-25 0.18901146661952595 1103 26-27 0.1983088247937701 1103 28-29 0.20262734613294953 1103 30-31 0.2652776517161044 1103 32-33 0.2680228157719782 1103 34-35 0.2698634914982705 1103 36-37 0.2533234120254093 1103 38-39 0.2788196840867272 1103 40-41 0.3883133631249436 1103 42-43 0.4075195614733431 1103 44-45 0.3627563113392682 1103 46-47 0.28534926776942626 1103 48-49 0.14170088111067258 1103 50-51 0.13371393448080937 1103 52-53 0.1420629310422683 1103 54-55 0.3177224799089089 1103 56-57 0.4307126623588786 1103 58-59 0.3042396355584742 1103 60-61 0.1488732405618549 1103 62-63 0.2556045754982321 1103 64-65 0.20852540192670688 1103 66-67 0.23364058936095233 1103 68-69 0.35829666218822354 1103 70-71 0.2868232116910505 1103 72-73 0.3074805268786136 1103 74-75 0.27247852378056336 1103 76-77 0.29194740291253396 1103 78-79 0.47204690090066137 1103 80-81 0.46706993219764215 1103 82-83 0.44561313921627743 1103 84-85 0.551318365938716 1103 86-87 0.6782073542679434 1103 88-89 0.6354224677120044 1103 90-91 0.5802162733055276 1103 92-93 0.8577084255686245 1103 94-95 1.015661102522401 1103 96-97 0.721956576090605 1103 98-99 1.0469403573640896 1103 100-101 1.1144536827843083 1104 1 0.0833525860109745 1104 2 0.08525317900055995 1104 3 0.08728503311130709 1104 4 0.05787859586512667 1104 5 0.031938497537133514 1104 6 0.07385514760986212 1104 7 0.1035207071228541 1104 8 0.1322379591528886 1104 9 0.15072967050651442 1104 10-11 0.19750284671494 1104 12-13 0.16791082685617909 1104 14-15 0.2071850216506732 1104 16-17 0.2039380730129139 1104 18-19 0.14152442868443416 1104 20-21 0.1727194206858016 1104 22-23 0.12159121046803278 1104 24-25 0.13703209448653553 1104 26-27 0.03947083876999358 1104 28-29 0.07789588471273845 1104 30-31 0.03840189521361026 1104 32-33 0.11342823471630226 1104 34-35 0.2064216952519189 1104 36-37 0.33223822093035693 1104 38-39 0.33056045585028215 1104 40-41 0.27339127389102913 1104 42-43 0.14651846996718376 1104 44-45 0.31717001078267515 1104 46-47 0.29124849089990335 1104 48-49 0.18491048258267284 1104 50-51 0.2559012641373002 1104 52-53 0.2618975524185885 1104 54-55 0.5112293410275015 1104 56-57 0.23271791136582465 1104 58-59 0.3777351380119214 1104 60-61 0.295856349124314 1104 62-63 0.26202812595823843 1104 64-65 0.20955001548614405 1104 66-67 0.17530657117482207 1104 68-69 0.19769784165060855 1104 70-71 0.3399999613524862 1104 72-73 0.3893827190345931 1104 74-75 0.4069533198059183 1104 76-77 0.22409391400076117 1104 78-79 0.47574807345248615 1104 80-81 0.5225527665004464 1104 82-83 0.4874476702624406 1104 84-85 0.5968795189015665 1104 86-87 0.8382457858266044 1104 88-89 0.7770642776587664 1104 90-91 0.8390463730438995 1104 92-93 0.7785966104143007 1104 94-95 0.9400052708809135 1104 96-97 0.820817048086802 1104 98-99 1.07654516091662 1104 100-101 0.9561737218656781 1105 1 0.004025406863846115 1105 2 -0.029645875037495273 1105 3 0.00412365064145348 1105 4 0.037445170045643295 1105 5 -0.018095959695813235 1105 6 -0.00654060309057769 1105 7 -0.006081760721166063 1105 8 0.043628501384297635 1105 9 0.024387153640780923 1105 10-11 0.024048727141753545 1105 12-13 0.14007953220473723 1105 14-15 0.04561887377215612 1105 16-17 -0.036370069506347136 1105 18-19 0.07328764014883404 1105 20-21 0.06945706358574455 1105 22-23 0.004427967679781375 1105 24-25 -0.03545560305912687 1105 26-27 -0.053679721181417506 1105 28-29 0.0025000791905469555 1105 30-31 -0.006596354010909522 1105 32-33 0.11991591154035319 1105 34-35 0.10950714405951345 1105 36-37 0.12414746547752742 1105 38-39 0.17223361981692165 1105 40-41 0.16209637298813817 1105 42-43 0.15537193182014875 1105 44-45 0.06049732311146272 1105 46-47 0.0354288096084332 1105 48-49 0.05325035613453366 1105 50-51 -0.0470051237438156 1105 52-53 -0.07604079151579413 1105 54-55 0.045212139469839485 1105 56-57 0.11286347368563554 1105 58-59 0.10427145229407131 1105 60-61 0.13932821988097288 1105 62-63 -0.011178752532309488 1105 64-65 -0.21368267952413333 1105 66-67 -0.0018032541007002578 1105 68-69 0.0145168339985986 1105 70-71 0.21905356485681438 1105 72-73 0.1229434603816344 1105 74-75 0.13121221770356684 1105 76-77 -0.09064884271266749 1105 78-79 0.08865523688539056 1105 80-81 0.272351510307729 1105 82-83 0.10421956619687123 1105 84-85 0.12433777414743119 1105 86-87 0.2504313440865893 1105 88-89 0.2906881374542891 1105 90-91 0.16421245529725326 1105 92-93 0.36820794831758974 1105 94-95 0.3185564254622655 1105 96-97 0.2935442311971954 1105 98-99 0.5941882097344369 1105 100-101 0.5298211660805201 1106 1 -0.018530059960710332 1106 2 0.033802876489254174 1106 3 -0.02272742666759342 1106 4 -0.0033989497229427457 1106 5 0.051964773044304025 1106 6 0.06656203432716978 1106 7 0.09096700878589559 1106 8 0.05629970948611884 1106 9 0.03620359425410413 1106 10-11 0.1390879993883587 1106 12-13 0.0494590777662367 1106 14-15 -0.07666204201603932 1106 16-17 -0.08000535279118282 1106 18-19 0.01313820684492839 1106 20-21 0.10599644223931648 1106 22-23 0.07838642612031066 1106 24-25 0.19696635287644426 1106 26-27 0.16508070441834377 1106 28-29 0.07975258818245123 1106 30-31 0.22016531335459888 1106 32-33 0.20918468148287417 1106 34-35 0.15197230192460154 1106 36-37 0.012311288655133978 1106 38-39 0.19298508475635145 1106 40-41 0.2902061428330356 1106 42-43 0.2749856499603922 1106 44-45 0.3255865680012917 1106 46-47 0.18359049762321433 1106 48-49 0.30547910792818556 1106 50-51 0.2401377544697283 1106 52-53 0.16122145213297046 1106 54-55 0.34663672853879035 1106 56-57 0.22063371049907232 1106 58-59 0.17644171186385194 1106 60-61 0.25728402622451796 1106 62-63 0.3226637890419113 1106 64-65 0.2688335677305247 1106 66-67 0.17983589073848805 1106 68-69 0.284451283094981 1106 70-71 0.3529781416345301 1106 72-73 0.4596972683813334 1106 74-75 0.5003502651112051 1106 76-77 0.2667286013990946 1106 78-79 0.5017818527436084 1106 80-81 0.620519659999502 1106 82-83 0.5924916224640278 1106 84-85 0.6162044894171466 1106 86-87 0.7624023030626681 1106 88-89 1.016443679571431 1106 90-91 1.2817777515967492 1106 92-93 1.191255275185032 1106 94-95 1.3540025866993695 1106 96-97 1.1403652617082471 1106 98-99 1.2595534103730195 1106 100-101 1.2817840760546098 1107 1 0.00961247385811248 1107 2 0.049391336679221354 1107 3 0.04089262547649497 1107 4 0.04267318110987617 1107 5 -0.057656810383100776 1107 6 -0.10551436016081084 1107 7 -0.08811298050368066 1107 8 -0.005715970385054447 1107 9 -0.15573736261574567 1107 10-11 -0.004854823141798192 1107 12-13 -0.03679559820921696 1107 14-15 -0.007541697466336927 1107 16-17 0.018607380997629264 1107 18-19 0.061699245632169664 1107 20-21 0.03858955572624723 1107 22-23 -0.016157821923293625 1107 24-25 -0.029205427876206613 1107 26-27 0.034151748774881696 1107 28-29 -0.08813070090418051 1107 30-31 -0.008902841299629927 1107 32-33 0.07832303937320972 1107 34-35 0.11498515811253185 1107 36-37 0.1105791899476074 1107 38-39 0.07673167439482853 1107 40-41 0.054306958989720044 1107 42-43 0.18123104144783753 1107 44-45 0.20139623480228863 1107 46-47 0.17498965024938684 1107 48-49 0.12465205362318699 1107 50-51 0.005666643178052766 1107 52-53 0.03806681133780643 1107 54-55 0.04692192482893631 1107 56-57 0.22461576407367545 1107 58-59 0.23236073726744166 1107 60-61 0.12379874951660241 1107 62-63 0.2859112468357736 1107 64-65 0.2276251882311655 1107 66-67 0.07550588793143476 1107 68-69 0.2314987830494175 1107 70-71 0.29052945451231693 1107 72-73 0.39837663061945605 1107 74-75 0.28296558907700486 1107 76-77 0.4221827352417016 1107 78-79 0.638844555001608 1107 80-81 0.43858576607188837 1107 82-83 0.2725115118208912 1107 84-85 0.5302990770804286 1107 86-87 0.5993762430661924 1107 88-89 0.6954526710065814 1107 90-91 0.7461195024078222 1107 92-93 1.0587577449245629 1107 94-95 1.2971706463877517 1107 96-97 1.451901257968963 1107 98-99 1.530731955837087 1107 100-101 1.382288409761653 1108 1 0.056912143926112435 1108 2 0.04431375122673131 1108 3 0.04888397058270755 1108 4 0.015425823201461242 1108 5 0.018678411175933718 1108 6 0.07490312383004749 1108 7 0.0442422079035083 1108 8 0.1387377482971246 1108 9 0.1446782380964251 1108 10-11 0.08717916389734626 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2316 68-69 -0.6813878653210139 2316 70-71 -0.6072764928624537 2316 72-73 -0.4703033503884484 2316 74-75 -0.749493479186988 2316 76-77 -0.6219392227310436 2316 78-79 -0.5578612542870438 2316 80-81 -0.7183079849916538 2316 82-83 -0.7658674952766624 2316 84-85 -1.127433591298992 2316 86-87 -1.1158801282900548 2316 88-89 -1.2009036851832775 2316 90-91 -1.1546403870746893 2316 92-93 -1.0248668694094292 2316 94-95 -1.045135839379899 2316 96-97 -1.0450428496262596 2316 98-99 -0.7322393038041533 2316 100-101 0.03320293009111097 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 7.0 3 9.0 4 29.0 5 91.0 6 217.0 7 413.0 8 1133.0 9 2045.0 10 3367.0 11 5115.0 12 7309.0 13 9958.0 14 12558.0 15 16784.0 16 22263.0 17 30095.0 18 30389.0 19 23846.0 20 23946.0 21 26019.0 22 28252.0 23 31479.0 24 36474.0 25 42329.0 26 49325.0 27 58977.0 28 70441.0 29 84438.0 30 101250.0 31 121127.0 32 148936.0 33 187510.0 34 247115.0 35 341923.0 36 489468.0 37 597854.0 38 283367.0 39 9007.0 40 1.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 41.58591750851959 20.24126769742188 15.587646001137434 22.585168792921095 2 15.534970329419442 21.285008645837376 39.979350471530424 23.20067055321276 3 19.471386062236036 24.831550851451222 30.448069965461166 25.24899312085157 4 13.401874674469436 17.80956644893614 36.91295590972716 31.875602966867266 5 13.692570685046675 37.48490396729145 34.40191092402665 14.42061442363522 6 31.451069775309982 36.77314073159238 17.293264641482338 14.4825248516153 7 27.707349057161736 33.198330230922394 20.96130645948031 18.133014252435554 8 25.299965085952785 36.197694909735425 19.67702280478723 18.82531719952456 9 25.708758338193107 17.485737071150247 20.652231287438003 36.15327330321864 10-11 24.654993249314916 27.00933839470426 27.399943272622746 20.935725083358083 12-13 25.748442063986193 24.306504633265774 28.000668390958474 21.944384911789562 14-15 22.419727569389515 25.73002579624543 26.55176936805161 25.29847726631344 16-17 21.776176607416158 28.964987409306143 27.1495998293046 22.109236153973104 18-19 21.624480003434183 27.70007496377016 29.39893047448171 21.276514558313945 20-21 22.458937837097036 26.66150163472784 29.395036227298714 21.484524300876412 22-23 22.078651363841896 26.702489072666367 29.327290892521336 21.8915686709704 24-25 22.0779976495009 26.8178051578701 29.38662848377545 21.717568708853545 26-27 22.035218994262234 26.807760141093475 29.453437674838558 21.703583189805734 28-29 21.724979697068175 27.15009160962661 29.448408930618985 21.676519762686233 30-31 22.02651877695266 26.843814649018434 29.286907613869715 21.84275896015919 32-33 21.814299877959826 27.005061582910052 29.317242769644242 21.863395769485887 34-35 22.243591231748578 26.882894188261908 29.20481949167446 21.66869508831505 36-37 22.166756171073057 26.79288033661078 29.275383245570247 21.764980246745917 38-39 22.010083522656444 26.988624076811334 29.3649047109017 21.63638768963052 40-41 22.159265987829002 26.928545239290752 29.19185331204453 21.720335460835713 42-43 21.957350977330375 27.05560393479281 28.917187871903792 22.069857215973023 44-45 22.168048463202492 27.018454798193474 28.762402542085887 22.051094196518147 46-47 22.26416756244806 27.241108313047413 28.48123755995349 22.013486564551034 48-49 22.380645598267346 27.29274895199113 28.340614727994673 21.985990721746848 50-51 22.130673959012352 27.44197656486001 28.405265751943652 22.022083724183982 52-53 22.368313944059935 27.59812341765913 27.98157377770627 22.051988860574664 54-55 22.133136992164374 27.310241517444624 28.134569167652927 22.422052322738075 56-57 22.3023810871433 27.557787835793324 28.019190642780966 22.120640434282414 58-59 22.5008402634532 27.320343406213404 28.08222466784553 22.09659166248787 60-61 22.309404278268037 27.237660994701134 28.471116348641452 21.981818378389377 62-63 22.19560357793585 27.347185698432032 28.385206831529846 22.072003892102277 64-65 22.07153293723538 27.46273663725875 28.22635137820783 22.239379047298037 66-67 22.09808939821028 27.476723836803963 28.01198933666413 22.413197428321627 68-69 21.939783655145614 27.485441017758237 28.14154122654775 22.4332341005484 70-71 22.144190268232457 27.305507978004144 28.043472765369458 22.50682898839394 72-73 22.16428299329765 27.187104315414395 28.100927034728983 22.547685656558976 74-75 21.97934665578756 27.628363179862035 27.795953786266253 22.596336378084153 76-77 22.118891584247823 27.25897908572965 27.96193470725034 22.660194622772185 78-79 22.023049757024538 27.34750674295861 28.003025348182152 22.626418151834702 80-81 22.025779860330736 27.471383445611853 27.905679684106566 22.59715700995085 82-83 21.986040104729422 27.37223144006772 28.088160195060773 22.553568260142086 84-85 22.016464961874295 27.297812939208743 27.92251483372869 22.763207265188274 86-87 21.966672278680065 27.213772199146486 28.00489104472244 22.814664477451004 88-89 21.966974101166002 27.154611964528165 27.83299230711263 23.045421627193203 90-91 21.897460653088906 27.16457281921366 27.922698529851786 23.015267997845644 92-93 21.948375542868916 27.258108930555387 27.868150821052474 22.925364705523226 94-95 21.847910472986133 27.16417711116167 27.864387948697523 23.12352446715467 96-97 21.657345654791015 27.085287576640788 27.94968052692865 23.30768624163955 98-99 21.657345654791015 27.099882793098338 27.641495694888114 23.601275857222532 100-101 21.891316348114316 26.23180677500881 28.626021192730217 23.25085568414666 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 2045.0 1 1415.5 2 858.0 3 955.5 4 829.5 5 810.5 6 1382.5 7 2981.5 8 4846.0 9 5441.0 10 4536.0 11 3490.5 12 3325.5 13 3576.0 14 3851.5 15 4045.5 16 4248.0 17 4453.5 18 4778.0 19 5217.0 20 5767.5 21 6810.0 22 8384.0 23 10168.5 24 12458.5 25 15618.0 26 19441.5 27 23700.5 28 28053.5 29 32623.0 30 37524.0 31 43306.0 32 48483.5 33 53648.5 34 60193.5 35 65853.0 36 71698.5 37 78845.5 38 84586.5 39 87893.5 40 91419.0 41 96531.5 42 101719.0 43 106425.0 44 111007.5 45 114312.0 46 116138.0 47 122629.5 48 136834.5 49 137644.5 50 125304.0 51 118184.5 52 111857.5 53 107032.5 54 102459.0 55 94040.0 56 85636.0 57 77586.5 58 68616.0 59 58992.5 60 49299.0 61 39526.0 62 30840.0 63 23825.0 64 17794.5 65 13094.0 66 9244.0 67 6718.0 68 4814.0 69 3044.5 70 1940.0 71 1226.5 72 749.0 73 447.0 74 299.5 75 212.5 76 131.0 77 70.0 78 36.0 79 21.5 80 14.5 81 8.5 82 8.0 83 5.5 84 2.5 85 1.0 86 1.0 87 0.5 88 0.5 89 0.5 90 0.0 91 0.0 92 0.5 93 0.5 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.3656753578689839 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 9.062389303709603E-4 16-17 0.002639222148097884 18-19 4.292710722809811E-4 20-21 0.0 22-23 0.0 24-25 6.35957144119972E-5 26-27 1.58989286029993E-5 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.012242175024309462 36-37 0.02227439897280202 38-39 0.01049329287797954 40-41 0.005628220725461753 42-43 0.004817375366708788 44-45 0.0014944992886819343 46-47 0.0010334303591949547 48-49 0.00295720072015787 50-51 5.087657152959776E-4 52-53 0.0 54-55 0.0 56-57 0.0 58-59 6.041592869139734E-4 60-61 0.0013355100026519412 62-63 0.0011447228594159496 64-65 6.677550013259706E-4 66-67 0.0022735467902289 68-69 0.002289445718831899 70-71 1.58989286029993E-5 72-73 0.0 74-75 0.0 76-77 0.08373965695199732 78-79 0.003306977149423855 80-81 2.543828576479888E-4 82-83 0.0 84-85 1.9078714323599163E-4 86-87 0.08893860660517809 88-89 0.4178874394012337 90-91 0.3713512753802547 92-93 0.0 94-95 8.744410731649616E-4 96-97 0.0 98-99 0.0 100-101 4.4517000088398044E-4 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 3144866.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 56.216133281987055 #Duplication Level Percentage of deduplicated Percentage of total 1 74.63446734986623 41.95661163970191 2 14.185249933590516 15.948798018100451 3 4.884375600431648 8.237421292594533 4 2.1864479111617943 4.916545887519745 5 1.186400624697626 3.334742782191722 6 0.7055811479208227 2.379902631166464 7 0.47821180875510205 1.8818253144597847 8 0.3411886725133923 1.5344246310653675 9 0.22701481134012025 1.1485705402153212 >10 1.0756782912879965 10.379332115555155 >50 0.06338131590469341 2.4667414934125316 >100 0.028832723495258873 2.998275563998175 >500 0.00186792318224117 0.6690563583123371 >1k 0.0010754709216825346 0.9766644527270684 >5k 1.1320746544026679E-4 0.4215252339385357 >10k+ 1.1320746544026679E-4 0.7495620450408504 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 12804 0.4071397636656061 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 10752 0.34189056067889695 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7102 0.22582838187700208 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 6145 0.1953978325308614 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 3.17978572059986E-5 0.0 7 0.0 0.0 0.0 3.17978572059986E-5 0.0 8 0.0 0.0 0.0 3.17978572059986E-5 0.0 9 0.0 0.0 0.0 6.35957144119972E-5 0.0 10-11 0.0 0.0 0.0 6.35957144119972E-5 0.0 12-13 0.0 0.0 0.0 9.53935716179958E-5 0.0 14-15 0.0 0.0 0.0 9.53935716179958E-5 0.0 16-17 0.0 0.0 0.0 9.53935716179958E-5 0.0 18-19 0.0 0.0 0.0 1.271914288239944E-4 0.0 20-21 0.0 0.0 0.0 1.9078714323599163E-4 0.0 22-23 0.0 0.0 0.0 3.8157428647198326E-4 0.0 24-25 0.0 0.0 0.0 5.564625011049755E-4 0.0 26-27 0.0 0.0 0.0 7.313507157379678E-4 0.0 28-29 0.0 0.0 0.0 0.0017170842891239247 0.0 30-31 1.58989286029993E-5 0.0 0.0 0.005389736796416763 0.0 32-33 3.17978572059986E-5 0.0 0.0 0.01295762681144443 0.0 34-35 3.17978572059986E-5 0.0 0.0 0.0249931157639149 0.0 36-37 3.17978572059986E-5 0.0 0.0 0.04399233544449907 0.0 38-39 3.17978572059986E-5 0.0 0.0 0.07140208835606986 0.0 40-41 3.17978572059986E-5 0.0 0.0 0.10299325949022947 0.0 42-43 3.17978572059986E-5 0.0 0.0 0.13698516884344197 0.0 44-45 3.17978572059986E-5 0.0 0.0 0.17992817500014308 0.0 46-47 3.17978572059986E-5 0.0 0.0 0.23264902224768877 0.0 48-49 3.17978572059986E-5 0.0 0.0 0.29060061700562123 0.0 50-51 3.17978572059986E-5 0.0 0.0 0.3530834064154085 0.0 52-53 3.17978572059986E-5 0.0 0.0 0.4193183429755036 0.0 54-55 3.17978572059986E-5 0.0 0.0 0.49674612527211015 0.0 56-57 3.17978572059986E-5 0.0 0.0 0.590295421172158 0.0 58-59 3.17978572059986E-5 0.0 0.0 0.6940677281639345 0.0 60-61 3.17978572059986E-5 0.0 0.0 0.8107817630385523 0.0 62-63 3.17978572059986E-5 0.0 0.0 0.9352385761428308 3.17978572059986E-5 64-65 3.17978572059986E-5 0.0 0.0 1.0679469331920661 3.17978572059986E-5 66-67 3.17978572059986E-5 0.0 0.0 1.2228978913568973 3.17978572059986E-5 68-69 3.17978572059986E-5 0.0 0.0 1.4045431506461643 3.17978572059986E-5 70-71 3.17978572059986E-5 0.0 0.0 1.6021509342528426 3.17978572059986E-5 72-73 3.17978572059986E-5 0.0 0.0 1.8093139739499233 3.17978572059986E-5 74-75 3.17978572059986E-5 0.0 0.0 2.0323123465355915 3.17978572059986E-5 76-77 3.17978572059986E-5 0.0 0.0 2.2719568973686 3.17978572059986E-5 78-79 3.17978572059986E-5 0.0 0.0 2.551269275066092 3.17978572059986E-5 80-81 3.17978572059986E-5 0.0 0.0 2.862220520683552 3.17978572059986E-5 82-83 3.17978572059986E-5 0.0 0.0 3.1800878002433173 3.17978572059986E-5 84-85 3.17978572059986E-5 0.0 0.0 3.5132498491191675 3.17978572059986E-5 86-87 3.17978572059986E-5 0.0 0.0 3.862899086956328 3.17978572059986E-5 88-89 3.17978572059986E-5 0.0 0.0 4.251961768800324 3.17978572059986E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTATCAA 19945 0.0 23.526503 1 GGTATCA 15105 0.0 22.108154 1 GTACATG 9890 0.0 22.060957 1 TACATGG 9660 0.0 21.616789 2 GAGTACT 10960 0.0 20.677555 12-13 ACATGGG 9860 0.0 20.215664 3 AGTACTT 11585 0.0 19.930727 12-13 ATCAACG 23350 0.0 19.776144 3 TCAACGC 23515 0.0 19.697926 4 TATCAAC 23600 0.0 19.68731 2 CAACGCA 23530 0.0 19.665197 5 AGAGTAC 17400 0.0 19.475351 10-11 AACGCAG 23950 0.0 19.379786 6 GTACTTT 11835 0.0 19.00844 14-15 ACGCAGA 26055 0.0 17.413355 7 CGCAGAG 26230 0.0 17.08006 8 CATGGGG 5150 0.0 16.863958 4 GTATAGG 940 0.0 16.724043 1 TACTTTT 13700 0.0 16.697937 14-15 GCAGAGT 26740 0.0 16.222368 9 >>END_MODULE