FastQCFastQC Report
Thu 26 May 2016
SRR938888_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938888_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3141822
Sequences flagged as poor quality0
Sequence length101
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT129700.41281778534875624No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT98560.3137033224670271No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT65710.20914615786635907No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT56420.1795773280599601No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA189700.024.7441391
GGTATCA140600.023.774331
GTACATG104100.022.2721751
GAGTACT100700.022.18762412-13
TACATGG102550.022.019562
ATCAACG218450.021.3456863
TCAACGC220850.021.1565914
TATCAAC221600.021.1277122
CAACGCA220750.021.1239595
ACATGGG104100.020.8276713
AACGCAG226000.020.6721786
GTACTTT109900.020.30935514-15
AGTACTT105850.019.54289212-13
GTGTAGC19050.018.9157311
ACGCAGA245250.018.721787
CGCAGAG245300.018.564188
AGAGTAC171050.018.54201310-11
GTATAGC10450.017.695091
GCAGAGT251400.017.4549929
TTTGCGT5750.017.28933
Produced by FastQC (version 0.11.5)