##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938888_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 3141822 Sequences flagged as poor quality 0 Sequence length 101 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.989441158665258 33.0 31.0 34.0 30.0 34.0 2 32.08644983706906 33.0 31.0 34.0 30.0 34.0 3 32.13662995548443 34.0 31.0 34.0 30.0 34.0 4 35.67182259211375 37.0 35.0 37.0 35.0 37.0 5 35.701433117471325 37.0 35.0 37.0 35.0 37.0 6 35.77273664771588 37.0 35.0 37.0 35.0 37.0 7 35.69878369939481 37.0 35.0 37.0 33.0 37.0 8 35.681008981412695 37.0 35.0 37.0 33.0 37.0 9 37.33070969647549 39.0 37.0 39.0 34.0 39.0 10-11 37.22237876620636 39.0 37.0 39.0 34.0 39.0 12-13 37.10026411426236 39.0 37.0 39.0 33.0 39.0 14-15 38.353457802510775 40.0 38.0 41.0 34.0 41.0 16-17 38.277421986350596 40.0 38.0 41.0 33.0 41.0 18-19 38.20830142509665 40.0 38.0 41.0 33.5 41.0 20-21 38.086836077919116 40.0 38.0 41.0 33.0 41.0 22-23 38.188508451465424 40.0 38.0 41.0 34.0 41.0 24-25 38.130539540432274 40.0 38.0 41.0 33.0 41.0 26-27 38.05065611610078 40.0 38.0 41.0 33.0 41.0 28-29 37.91949146068746 40.0 38.0 41.0 33.0 41.0 30-31 37.74768796577273 40.0 37.5 41.0 33.0 41.0 32-33 37.56149059367462 40.0 37.0 41.0 32.5 41.0 34-35 37.2594674364111 40.0 37.0 41.0 31.5 41.0 36-37 37.006873718498376 40.0 36.5 41.0 31.0 41.0 38-39 36.81074230175993 40.0 36.0 41.0 30.5 41.0 40-41 36.67200799408751 39.0 36.0 41.0 30.0 41.0 42-43 36.47354974279256 39.0 36.0 41.0 30.0 41.0 44-45 36.413316540529664 39.5 36.0 41.0 30.0 41.0 46-47 36.19561165463861 40.0 36.0 41.0 29.5 41.0 48-49 36.03134884789781 39.0 36.0 41.0 28.5 41.0 50-51 34.8990304033774 38.0 34.0 40.0 26.5 40.5 52-53 35.0648037985602 38.5 34.5 39.5 27.5 40.5 54-55 35.55851779636147 39.0 35.0 40.5 28.0 41.0 56-57 35.45750172988794 39.0 35.0 41.0 27.0 41.0 58-59 35.25790608124839 39.0 35.0 41.0 27.0 41.0 60-61 34.99061897841444 38.0 35.0 40.0 26.0 41.0 62-63 34.645814116776826 38.0 34.0 40.0 26.0 41.0 64-65 34.24696004420365 37.5 34.0 40.0 25.5 41.0 66-67 33.863255143034834 37.0 34.0 39.5 24.5 41.0 68-69 33.47001501039843 36.0 33.0 39.0 23.0 41.0 70-71 33.04726875042571 36.0 33.0 39.0 22.0 40.5 72-73 32.6032456644584 35.0 32.5 38.5 21.5 40.0 74-75 32.13027122478613 35.0 32.0 37.5 20.0 39.5 76-77 31.704495671619846 35.0 32.0 37.0 18.5 39.0 78-79 31.26609273217897 35.0 31.0 36.5 17.5 39.0 80-81 30.815144365275945 35.0 31.0 36.0 13.5 37.5 82-83 30.422184324891735 34.0 31.0 35.5 7.5 37.0 84-85 30.064595639090946 34.0 30.5 35.0 6.5 37.0 86-87 29.66511279124024 34.0 30.0 35.0 2.0 36.0 88-89 29.24919043790514 34.0 29.5 35.0 2.0 36.0 90-91 28.819747267668248 34.0 29.0 35.0 2.0 36.0 92-93 28.392253284877377 34.0 29.0 35.0 2.0 35.0 94-95 27.909042905677026 34.0 28.5 35.0 2.0 35.0 96-97 27.327220319929012 34.0 27.0 35.0 2.0 35.0 98-99 26.603843406787526 33.0 25.5 35.0 2.0 35.0 100-101 23.888777435513532 31.0 12.5 33.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.2746763926858016 1101 2 0.3291251675782618 1101 3 0.21928659482071566 1101 4 0.21728659327602173 1101 5 0.1223780426723664 1101 6 0.18506678466393112 1101 7 0.15929623779595659 1101 8 0.24958642838832645 1101 9 0.24738643695234686 1101 10-11 0.2625674724735205 1101 12-13 0.409468814206285 1101 14-15 0.2797404942950479 1101 16-17 0.4500913469551051 1101 18-19 0.2868942314727576 1101 20-21 0.37901701308802416 1101 22-23 0.4329039781332469 1101 24-25 0.4516582410186132 1101 26-27 0.4350061561596519 1101 28-29 0.46625374901722694 1101 30-31 0.49092855617003295 1101 32-33 0.4134554982281742 1101 34-35 0.44651167894430444 1101 36-37 0.34215055321214294 1101 38-39 0.45257287313078365 1101 40-41 0.608329733743247 1101 42-43 0.3884990519081626 1101 44-45 0.5215647357493225 1101 46-47 0.5788400276137864 1101 48-49 0.4974159946183221 1101 50-51 0.5470444000918633 1101 52-53 0.49618096525020405 1101 54-55 0.49704317466028414 1101 56-57 0.4476451972515676 1101 58-59 0.37560678244017254 1101 60-61 0.27673730992914614 1101 62-63 0.2752415745017984 1101 64-65 0.3250241683862143 1101 66-67 0.3222040428023121 1101 68-69 0.3605429027968938 1101 70-71 0.25436561402887037 1101 72-73 0.18528325971138315 1101 74-75 0.18136429152583133 1101 76-77 0.16652915892005637 1101 78-79 0.1481379080786276 1101 80-81 0.07630522446207166 1101 82-83 -0.09863053311265801 1101 84-85 -0.1133874589822561 1101 86-87 -0.06844877139396743 1101 88-89 -0.046489947510657714 1101 90-91 -0.2752465707288785 1101 92-93 -0.09188761439933657 1101 94-95 -0.09794116882726556 1101 96-97 -0.19233443526517746 1101 98-99 -0.10021023524289774 1101 100-101 0.29758621339676594 1102 1 0.276457305802996 1102 2 0.20543950434133507 1102 3 0.1849788268320367 1102 4 0.21283028393414583 1102 5 0.210763344511804 1102 6 0.2615433226018311 1102 7 0.3656533335621148 1102 8 0.20054640184059735 1102 9 0.3620444793405113 1102 10-11 0.3025022443454617 1102 12-13 0.42347599933727764 1102 14-15 0.4868959270375939 1102 16-17 0.4626773567404072 1102 18-19 0.4777838306710649 1102 20-21 0.46731490935371767 1102 22-23 0.48169651923620194 1102 24-25 0.48504874152551025 1102 26-27 0.5524824390500456 1102 28-29 0.4230753955869986 1102 30-31 0.32856744878265687 1102 32-33 0.3498097056820626 1102 34-35 0.42609982584498596 1102 36-37 0.4593175775051037 1102 38-39 0.48010381200315777 1102 40-41 0.42229517043671194 1102 42-43 0.36847964001411526 1102 44-45 0.5541794381328558 1102 46-47 0.5347680258483933 1102 48-49 0.5296865252791676 1102 50-51 0.40623176199228794 1102 52-53 0.3604718305722514 1102 54-55 0.42806107541999694 1102 56-57 0.3991054939571441 1102 58-59 0.3424806101374642 1102 60-61 0.28699130954436924 1102 62-63 0.23841795001263932 1102 64-65 0.26305739762835145 1102 66-67 0.27173802869191377 1102 68-69 0.2664959785744827 1102 70-71 0.3646225402576846 1102 72-73 0.2398507067134048 1102 74-75 0.14275636572589434 1102 76-77 0.22252121856562113 1102 78-79 0.20437652018810937 1102 80-81 0.06686223072099651 1102 82-83 0.08246350379429401 1102 84-85 0.02240721530774792 1102 86-87 0.05712028554015802 1102 88-89 0.12348114088613471 1102 90-91 0.1071765317624056 1102 92-93 0.11749184825672998 1102 94-95 0.1752035079228591 1102 96-97 0.11508078844840597 1102 98-99 -0.049121775597217976 1102 100-101 0.21014174908146543 1103 1 0.07323430634193429 1103 2 0.21496648814628827 1103 3 0.1850112597046163 1103 4 0.1411881385893352 1103 5 0.1737629573875168 1103 6 0.16473050789083032 1103 7 0.1890653131611515 1103 8 0.10961773828886834 1103 9 0.19816117419617285 1103 10-11 0.23995993497312185 1103 12-13 0.35117277544073744 1103 14-15 0.355499664270603 1103 16-17 0.3565255998793049 1103 18-19 0.31113993602131984 1103 20-21 0.33964497662777404 1103 22-23 0.3622431442507761 1103 24-25 0.3929662957832889 1103 26-27 0.3004334750733122 1103 28-29 0.22598117395178718 1103 30-31 0.2938045848358044 1103 32-33 0.37236504340832255 1103 34-35 0.3060112495997913 1103 36-37 0.3691962854622659 1103 38-39 0.3829801247581699 1103 40-41 0.48596089931032793 1103 42-43 0.41578747006862216 1103 44-45 0.5257823880061281 1103 46-47 0.4930200182273623 1103 48-49 0.43026838481446106 1103 50-51 0.34634894817146744 1103 52-53 0.457502359566206 1103 54-55 0.6097458872437755 1103 56-57 0.49970570911821 1103 58-59 0.47710566887892014 1103 60-61 0.3576339013587173 1103 62-63 0.4682324546833243 1103 64-65 0.3017819024577406 1103 66-67 0.27177218671728554 1103 68-69 0.3174625126804145 1103 70-71 0.30560057768246196 1103 72-73 0.34633783327820566 1103 74-75 0.2864194999696963 1103 76-77 0.12341533075713684 1103 78-79 0.31238247127519614 1103 80-81 0.3062109648383746 1103 82-83 0.2816154876855066 1103 84-85 0.2945894024840676 1103 86-87 0.29684717315059217 1103 88-89 0.3526970175363324 1103 90-91 0.36046657066115273 1103 92-93 0.35052686816844414 1103 94-95 0.39288432291264286 1103 96-97 0.3800194040615885 1103 98-99 0.19683360367072567 1103 100-101 0.26803753191095225 1104 1 0.10687482239151436 1104 2 0.12199118973613565 1104 3 0.17546896708602588 1104 4 0.20106842895559396 1104 5 0.23004457340240236 1104 6 0.2399965923795051 1104 7 0.16507856095882545 1104 8 0.2322948368458242 1104 9 0.27546059657971966 1104 10-11 0.20751825298780346 1104 12-13 0.3040739104330825 1104 14-15 0.3456177744057527 1104 16-17 0.2933695462806938 1104 18-19 0.3780361011148443 1104 20-21 0.3500000021239842 1104 22-23 0.38192872627178787 1104 24-25 0.4510903465746807 1104 26-27 0.39577973393497956 1104 28-29 0.3287419480064173 1104 30-31 0.43044747821421225 1104 32-33 0.39397303513516135 1104 34-35 0.2545057166182261 1104 36-37 0.30022605450059103 1104 38-39 0.3360905274822983 1104 40-41 0.4044539070331368 1104 42-43 0.40817533781259385 1104 44-45 0.5229966933685688 1104 46-47 0.7460020957469524 1104 48-49 0.7402065335863952 1104 50-51 0.6986948399383266 1104 52-53 0.6646908912798182 1104 54-55 0.7341105061770676 1104 56-57 0.7155975372009351 1104 58-59 0.6557575166848508 1104 60-61 0.6412553122862974 1104 62-63 0.6171270135643212 1104 64-65 0.7031736538237752 1104 66-67 0.7123525914378277 1104 68-69 0.5873662979036922 1104 70-71 0.658205449992117 1104 72-73 0.6012521445844712 1104 74-75 0.5718508620296276 1104 76-77 0.42955758305256353 1104 78-79 0.4197796684402526 1104 80-81 0.30560646298048866 1104 82-83 0.25017813283753654 1104 84-85 0.27351921747342445 1104 86-87 0.41347337842708143 1104 88-89 0.4428379452099662 1104 90-91 0.5329012563211748 1104 92-93 0.571677936465651 1104 94-95 0.6243249557453723 1104 96-97 0.5513183963577326 1104 98-99 0.4870187841385949 1104 100-101 1.0993809211536707 1105 1 0.07873585627796231 1105 2 0.13212306129349116 1105 3 0.16025153077472254 1105 4 0.13447542140205115 1105 5 0.14731420442844723 1105 6 0.24181417689445084 1105 7 0.16992349986681887 1105 8 0.16743152287323682 1105 9 0.22828543072952812 1105 10-11 0.1853252062153743 1105 12-13 0.2924149827259157 1105 14-15 0.28837421378386097 1105 16-17 0.2915222741613803 1105 18-19 0.28899154978634556 1105 20-21 0.25851432893215787 1105 22-23 0.19639448025295536 1105 24-25 0.2792622034288641 1105 26-27 0.2481505039884624 1105 28-29 0.3760140868537789 1105 30-31 0.34273877142579323 1105 32-33 0.23350254910611312 1105 34-35 0.30357961961242097 1105 36-37 0.22154267241057823 1105 38-39 0.27575877240972346 1105 40-41 0.2951208078152092 1105 42-43 0.2444923404895576 1105 44-45 0.13734748600807478 1105 46-47 0.3559925214945494 1105 48-49 0.28964869959401085 1105 50-51 0.2974426480360961 1105 52-53 0.1684737170788182 1105 54-55 0.22296730293923872 1105 56-57 0.1573320003299088 1105 58-59 0.1396268935693783 1105 60-61 0.2988044989826619 1105 62-63 0.2321997813982719 1105 64-65 0.2085271882697981 1105 66-67 -0.003418577288940128 1105 68-69 0.09777990873278952 1105 70-71 -0.03418760920222752 1105 72-73 0.09666833940702446 1105 74-75 0.07658701149265568 1105 76-77 0.009581790168915916 1105 78-79 0.05419039488421262 1105 80-81 -0.021212850056155474 1105 82-83 -0.056729489103322095 1105 84-85 0.07191675090225047 1105 86-87 0.159531860663936 1105 88-89 0.13678872472442194 1105 90-91 0.14190183483883345 1105 92-93 0.22424839472116886 1105 94-95 0.28306643178962787 1105 96-97 0.1900090206607068 1105 98-99 0.21924811619484075 1105 100-101 0.41178807414756946 1106 1 0.29155212545779463 1106 2 0.2538632526932929 1106 3 0.3012021537194798 1106 4 0.2610035596916518 1106 5 0.272463225093567 1106 6 0.30319711228177226 1106 7 0.3193164952559826 1106 8 0.3098669406919541 1106 9 0.34880039576944455 1106 10-11 0.304616617626543 1106 12-13 0.31440736294360505 1106 14-15 0.3424776200548294 1106 16-17 0.44474011688922843 1106 18-19 0.43354198853388226 1106 20-21 0.5055539884034559 1106 22-23 0.4132049362001027 1106 24-25 0.5275137074109537 1106 26-27 0.5395016968425566 1106 28-29 0.5176665681044312 1106 30-31 0.3807276762985552 1106 32-33 0.5453726428380961 1106 34-35 0.463129733653318 1106 36-37 0.32673010685427073 1106 38-39 0.3587552636101705 1106 40-41 0.40904186671305354 1106 42-43 0.4089751418615961 1106 44-45 0.5418555948182515 1106 46-47 0.4053842377363779 1106 48-49 0.4455620009552206 1106 50-51 0.31504469939484636 1106 52-53 0.30388474647756425 1106 54-55 0.21611372988713384 1106 56-57 0.38487155058169975 1106 58-59 0.6142526445764602 1106 60-61 0.603020175031709 1106 62-63 0.47127635509388455 1106 64-65 0.2361935709001628 1106 66-67 0.5322342109570712 1106 68-69 0.5691416693118327 1106 70-71 0.567235196223713 1106 72-73 0.4649402703881762 1106 74-75 0.4431858177529975 1106 76-77 0.5053887451881991 1106 78-79 0.5532681296520288 1106 80-81 0.5958479251858471 1106 82-83 0.6034562627696438 1106 84-85 0.6120602318754287 1106 86-87 0.6310673884893241 1106 88-89 0.7098371886652615 1106 90-91 0.666367594032458 1106 92-93 0.7398855773696305 1106 94-95 0.7778421972609166 1106 96-97 0.7319858149600442 1106 98-99 0.8783265200551114 1106 100-101 1.267944118315544 1107 1 0.01679917732051095 1107 2 -0.045781546442910326 1107 3 0.01522930078718332 1107 4 0.06643586185343509 1107 5 -0.01205346366939608 1107 6 -0.10752824055341392 1107 7 -0.12366440298584536 1107 8 0.045253951257336666 1107 9 0.021055184157980023 1107 10-11 0.045879333787588905 1107 12-13 0.05098792212100989 1107 14-15 -0.0506005948566326 1107 16-17 0.13898852241984372 1107 18-19 0.07307805596427386 1107 20-21 0.10068895178270765 1107 22-23 -0.04328400386498288 1107 24-25 0.08433389270108904 1107 26-27 0.17362105070768052 1107 28-29 0.18021887456927033 1107 30-31 0.19550486893675156 1107 32-33 0.260661968185552 1107 34-35 0.04212188357477942 1107 36-37 0.06513214124082367 1107 38-39 0.0976402954696809 1107 40-41 0.03874481679269337 1107 42-43 0.09406965457601046 1107 44-45 0.30532399279860556 1107 46-47 0.4091167354279648 1107 48-49 0.24678971498963875 1107 50-51 0.2150139704362175 1107 52-53 0.16215302771774986 1107 54-55 0.1889199462010751 1107 56-57 0.18091911920888037 1107 58-59 0.0885750884699732 1107 60-61 0.0756629351994107 1107 62-63 0.0031540388973780864 1107 64-65 0.12490787525530322 1107 66-67 0.1257849645387168 1107 68-69 0.16820192098050768 1107 70-71 0.1489939983023234 1107 72-73 0.08153799196289668 1107 74-75 0.07132537603293798 1107 76-77 0.14075757735827565 1107 78-79 0.26704939524685045 1107 80-81 0.1821492841329544 1107 82-83 0.2167089603937029 1107 84-85 0.26760175382048246 1107 86-87 0.31276518088507643 1107 88-89 0.3986513496850037 1107 90-91 0.45008607692832925 1107 92-93 0.3432860827788282 1107 94-95 0.44617319147470624 1107 96-97 0.4237308313405208 1107 98-99 0.48569693797891134 1107 100-101 0.46927388038276163 1108 1 0.15238000038497646 1108 2 0.10622867498229738 1108 3 0.10351242433375063 1108 4 0.14196550656102147 1108 5 0.08250601466018992 1108 6 0.15179503979874198 1108 7 0.1967819784405691 1108 8 0.1330525014243733 1108 9 0.19809369186734926 1108 10-11 0.2397648053475976 1108 12-13 0.2104533690982322 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-2.2862761991936473 2316 88-89 -2.2836074291818385 2316 90-91 -2.5795535501838565 2316 92-93 -2.81576632827689 2316 94-95 -2.978814306465445 2316 96-97 -3.2291445566474586 2316 98-99 -3.6891372328121435 2316 100-101 -4.319632496511243 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 9641.0 3 2419.0 4 2385.0 5 2570.0 6 2945.0 7 3595.0 8 4359.0 9 5168.0 10 6514.0 11 8332.0 12 11038.0 13 17095.0 14 16543.0 15 14608.0 16 19268.0 17 25619.0 18 22191.0 19 16048.0 20 15731.0 21 16877.0 22 18127.0 23 20491.0 24 23188.0 25 26607.0 26 31720.0 27 37013.0 28 44674.0 29 54008.0 30 66068.0 31 82398.0 32 104305.0 33 137226.0 34 189300.0 35 275947.0 36 422702.0 37 661028.0 38 620688.0 39 103348.0 40 38.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.020546839146675 19.129412981190555 14.750404190173949 23.09963598948882 2 14.929524165077407 20.293071423816052 41.14435592868863 23.633048482417912 3 19.33771087756988 24.4137126525427 29.958941807841033 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26.569087117428424 29.207510677990673 22.05148058047485 24-25 22.174534092710633 26.668881384341947 29.283538832047125 21.873045690900298 26-27 22.08151086185301 26.716227587205772 29.29167037786683 21.91059117307439 28-29 21.782341800699083 27.049456793715127 29.334111257989886 21.83409014759591 30-31 22.059633686701858 26.814814649600553 29.134645929963764 21.990905733733825 32-33 21.846994634758463 26.970426732568065 29.1451027096708 22.037475923002674 34-35 22.25921634052123 26.914389718172966 29.02555682182101 21.80083711948479 36-37 22.18517842796465 26.741597129532718 29.09769767270656 21.975526769796076 38-39 22.05100403778499 26.942861998129118 29.180941219617807 21.825192744468087 40-41 22.17611731877086 26.941648531555895 29.020656031134006 21.861578118539242 42-43 21.979620541503465 26.994166669199952 28.806260746798817 22.219952042497766 44-45 22.16031168854446 26.963248315437106 28.692747825903268 22.18369217011517 46-47 22.278861764420682 27.195797014206985 28.39153194306966 22.133809278302678 48-49 22.32385556854425 27.214921237713195 28.322029753361306 22.139193440381245 50-51 22.114035713817042 27.394099932241623 28.416348812564074 22.075515541377257 52-53 22.32636692793621 27.541520604103663 27.991167109228765 22.14094535873136 54-55 22.096042476448645 27.22221244305189 28.23318840602902 22.448556674470442 56-57 22.25119151017305 27.48391735236528 28.05619099742892 22.208700140032743 58-59 22.446500664927335 27.288624215512925 28.10678958557262 22.15808553398712 60-61 22.31389683104886 27.14365568607311 28.47881263384907 22.06363484902896 62-63 22.12562442597612 27.2666062908217 28.45356766841501 22.154201614787176 64-65 22.053296814457994 27.387107761413432 28.297294632544823 22.262300791583755 66-67 22.06254206064673 27.40801404593487 28.023836340782964 22.505607552635436 68-69 21.900138758422674 27.396992521644588 28.2446067027938 22.45826201713894 70-71 22.100179798072933 27.284005399064643 28.086005332472762 22.529809470389665 72-73 22.11798469489835 27.101714421080768 28.25484706902982 22.52545381499106 74-75 21.92818157081806 27.558781631640443 27.967123901595787 22.54591289594571 76-77 22.130439458369846 27.233123348054285 27.974414485463516 22.662022708112353 78-79 22.08409139599743 27.25033611822529 28.06356382438301 22.60200866139427 80-81 22.060456153009888 27.4420889187185 27.914353116602925 22.583101811668694 82-83 22.083777900524904 27.30979191375215 28.120797679951302 22.485632505771637 84-85 22.089143002720597 27.244325605741597 27.96804221096103 22.698489180576775 86-87 22.049594774868794 27.158908512359968 28.137964559982425 22.653532152788813 88-89 22.05368270857565 27.080251366840802 27.944844988954532 22.921220935629016 90-91 22.04760566410026 27.079062573224462 28.033317370667195 22.840014392008083 92-93 22.120434247221528 27.121179356113874 28.032373100592583 22.72601329607202 94-95 22.076785408733112 26.999327567046016 27.984799589849512 22.93908743437136 96-97 21.90772361035372 26.93038239591708 28.127273341318155 23.034620652411046 98-99 21.914319016994764 26.942079125352503 27.85734796926876 23.286253888383975 100-101 22.135771527772555 26.399405835733937 28.316645001093814 23.148177635399694 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 4518.0 1 2692.0 2 1013.5 3 1152.5 4 987.0 5 958.5 6 1610.0 7 3343.5 8 5333.0 9 6038.5 10 5059.5 11 4106.0 12 4161.0 13 4423.5 14 4739.0 15 4978.5 16 5250.5 17 5524.0 18 5667.5 19 5910.0 20 6399.0 21 7161.5 22 8283.5 23 9851.0 24 12099.0 25 15014.0 26 18646.0 27 23059.0 28 27367.5 29 31782.5 30 36603.0 31 42240.0 32 47689.0 33 52896.5 34 58929.0 35 64596.0 36 70879.5 37 78013.5 38 83793.0 39 87510.0 40 90719.0 41 95644.5 42 100712.0 43 104841.0 44 109766.0 45 113467.0 46 115261.5 47 121304.5 48 136136.5 49 136772.5 50 124085.0 51 117820.5 52 111771.5 53 107406.5 54 102615.5 55 94438.0 56 86453.5 57 78032.0 58 68925.5 59 59749.5 60 50125.5 61 39959.0 62 30988.0 63 23943.0 64 18092.5 65 13400.5 66 9500.5 67 7003.5 68 5115.0 69 3294.5 70 2150.5 71 1436.5 72 932.0 73 582.5 74 394.5 75 288.5 76 188.5 77 111.5 78 76.0 79 60.0 80 43.5 81 27.0 82 26.0 83 28.5 84 23.0 85 16.5 86 13.5 87 10.0 88 9.0 89 8.5 90 7.5 91 5.0 92 4.5 93 4.5 94 2.5 95 3.5 96 3.0 97 0.5 98 1.0 99 0.5 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.2672334715333968 2 0.08017640719302367 3 0.0833592736953271 4 0.09812777426601506 5 0.10016480882748928 6 0.07584770874989098 7 0.0853963082568013 8 0.08234075641459002 9 0.08717871349809124 10-11 0.08082889482599587 12-13 0.08488704961643276 14-15 0.09293970186726046 16-17 0.08186332643924449 18-19 0.09282830153967984 20-21 0.09419693413567032 22-23 0.1068488284823265 24-25 0.11854586287829165 26-27 0.1209011840899962 28-29 0.11323047581944491 30-31 0.10697614314241863 32-33 0.10164484175106037 34-35 0.36426315685611727 36-37 0.5599298750852213 38-39 0.543920056578635 40-41 0.5086061527355783 42-43 0.5335598261136373 44-45 0.5544871733662824 46-47 0.543983713908681 48-49 0.5645768601785842 50-51 0.5565242079277566 52-53 0.5598662177551752 54-55 0.5495855589527351 56-57 0.5245841425771416 58-59 0.518568524887788 60-61 0.4678495471735827 62-63 0.5395595294704792 64-65 0.5715791664836519 66-67 0.5842469751628195 68-69 0.5859020657440173 70-71 0.5830374858919443 72-73 0.5737753443702412 74-75 0.5720247677939743 76-77 0.5562377499425493 78-79 0.5348966300446046 80-81 0.5304087882763568 82-83 0.542997025292967 84-85 0.5514475358565826 86-87 0.5435540269308701 88-89 0.5558558059622729 90-91 0.5686509293015327 92-93 0.5766558385548258 94-95 0.575796464599204 96-97 0.572231654116624 98-99 0.5759396935918075 100-101 0.5548850316790703 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 3141822.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 57.015603791106685 #Duplication Level Percentage of deduplicated Percentage of total 1 75.31808287765399 42.943059716580564 2 13.803059152137395 15.739795034469491 3 4.733631782643676 8.096726226366059 4 2.1465183554289697 4.8954016033390415 5 1.1548809337394328 3.2923116871995415 6 0.731840209334354 2.5035786848284864 7 0.44904185278419895 1.7921674654777837 8 0.30593570051228075 1.3954486948770302 9 0.22606642849142952 1.160038252560411 >10 1.0423568526961826 10.300686931437994 >50 0.05709846842266091 2.2418306011114018 >100 0.02824292202091158 2.862775544116366 >500 0.0020138053260209763 0.7239933368911325 >1k 0.001006902661533645 0.9346530709767082 >5k 1.678171102556075E-4 0.7038682348877316 >10k+ 5.593903675186916E-5 0.4136649148803244 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 12970 0.41281778534875624 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 9856 0.3137033224670271 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6571 0.20914615786635907 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 5642 0.1795773280599601 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 3.1828665023034404E-5 0.0 3 0.0 0.0 0.0 3.1828665023034404E-5 0.0 4 0.0 0.0 0.0 9.548599506910322E-5 0.0 5 0.0 0.0 0.0 9.548599506910322E-5 0.0 6 0.0 0.0 0.0 9.548599506910322E-5 0.0 7 0.0 0.0 0.0 9.548599506910322E-5 0.0 8 0.0 0.0 0.0 9.548599506910322E-5 0.0 9 0.0 0.0 0.0 9.548599506910322E-5 0.0 10-11 0.0 0.0 0.0 9.548599506910322E-5 0.0 12-13 0.0 0.0 0.0 9.548599506910322E-5 0.0 14-15 0.0 0.0 0.0 1.1140032758062042E-4 0.0 16-17 0.0 0.0 0.0 1.5914332511517203E-4 0.0 18-19 0.0 0.0 0.0 1.9097199013820644E-4 0.0 20-21 0.0 0.0 0.0 2.3871498767275804E-4 0.0 22-23 0.0 0.0 0.0 3.5011531525337843E-4 0.0 24-25 0.0 0.0 0.0 4.6151564283399883E-4 0.0 26-27 0.0 0.0 0.0 7.638879605528257E-4 0.0 28-29 1.5914332511517202E-5 0.0 0.0 0.0020847775590087537 0.0 30-31 3.1828665023034404E-5 0.0 0.0 0.00631799000707233 0.0 32-33 3.1828665023034404E-5 0.0 0.0 0.014386556590411551 0.0 34-35 3.1828665023034404E-5 0.0 0.0 0.028009225220270276 0.0 36-37 3.1828665023034404E-5 0.0 0.0 0.04885700081035781 0.0 38-39 3.1828665023034404E-5 0.0 0.0 0.07961940555512056 0.0 40-41 3.1828665023034404E-5 0.0 0.0 0.11257798818647269 0.0 42-43 3.1828665023034404E-5 0.0 0.0 0.14835340767236335 3.1828665023034404E-5 44-45 3.1828665023034404E-5 0.0 0.0 0.19175179243127077 3.1828665023034404E-5 46-47 3.1828665023034404E-5 0.0 0.0 0.24614698095563659 3.1828665023034404E-5 48-49 3.1828665023034404E-5 0.0 0.0 0.30736941812744323 3.1828665023034404E-5 50-51 3.1828665023034404E-5 0.0 0.0 0.3713291204912309 3.1828665023034404E-5 52-53 3.1828665023034404E-5 0.0 0.0 0.44003129394345064 3.1828665023034404E-5 54-55 3.1828665023034404E-5 0.0 0.0 0.5198894144862439 3.1828665023034404E-5 56-57 3.1828665023034404E-5 0.0 0.0 0.6185901047226736 3.1828665023034404E-5 58-59 3.1828665023034404E-5 0.0 0.0 0.7297358029831098 3.1828665023034404E-5 60-61 3.1828665023034404E-5 0.0 0.0 0.8522761633217923 3.1828665023034404E-5 62-63 3.1828665023034404E-5 0.0 0.0 0.9799091100641601 3.1828665023034404E-5 64-65 3.1828665023034404E-5 0.0 0.0 1.1157697667149824 3.1828665023034404E-5 66-67 3.1828665023034404E-5 0.0 0.0 1.2761862384310758 3.1828665023034404E-5 68-69 3.1828665023034404E-5 0.0 0.0 1.4614927261951822 3.1828665023034404E-5 70-71 3.1828665023034404E-5 0.0 0.0 1.6669149302538462 3.1828665023034404E-5 72-73 3.1828665023034404E-5 0.0 0.0 1.8772546630585691 3.1828665023034404E-5 74-75 3.1828665023034404E-5 0.0 0.0 2.1023151534364457 3.1828665023034404E-5 76-77 3.1828665023034404E-5 0.0 0.0 2.352329317192381 3.1828665023034404E-5 78-79 3.1828665023034404E-5 0.0 0.0 2.639073760384898 3.1828665023034404E-5 80-81 3.1828665023034404E-5 0.0 0.0 2.9570102952999884 3.1828665023034404E-5 82-83 3.1828665023034404E-5 0.0 0.0 3.2823947378304688 3.1828665023034404E-5 84-85 3.1828665023034404E-5 0.0 0.0 3.6231365112345637 3.1828665023034404E-5 86-87 3.1828665023034404E-5 0.0 0.0 3.9866039514651055 3.1828665023034404E-5 88-89 3.1828665023034404E-5 0.0 0.0 4.386260583826837 3.1828665023034404E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTATCAA 18970 0.0 24.744139 1 GGTATCA 14060 0.0 23.77433 1 GTACATG 10410 0.0 22.272175 1 GAGTACT 10070 0.0 22.187624 12-13 TACATGG 10255 0.0 22.01956 2 ATCAACG 21845 0.0 21.345686 3 TCAACGC 22085 0.0 21.156591 4 TATCAAC 22160 0.0 21.127712 2 CAACGCA 22075 0.0 21.123959 5 ACATGGG 10410 0.0 20.827671 3 AACGCAG 22600 0.0 20.672178 6 GTACTTT 10990 0.0 20.309355 14-15 AGTACTT 10585 0.0 19.542892 12-13 GTGTAGC 1905 0.0 18.915731 1 ACGCAGA 24525 0.0 18.72178 7 CGCAGAG 24530 0.0 18.56418 8 AGAGTAC 17105 0.0 18.542013 10-11 GTATAGC 1045 0.0 17.69509 1 GCAGAGT 25140 0.0 17.454992 9 TTTGCGT 575 0.0 17.2893 3 >>END_MODULE