FastQCFastQC Report
Thu 26 May 2016
SRR938888_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938888_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3141822
Sequences flagged as poor quality0
Sequence length101
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT126750.4034283291669611No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT108140.34419518355909406No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT73990.23550029250543153No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT62130.19775149578811274No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA209850.022.9233591
GGTATCA161600.021.4858261
GTACATG105450.021.138061
GAGTACT110200.020.6278912-13
TACATGG104600.020.414572
ATCAACG242650.019.692853
TATCAAC244350.019.6141032
CAACGCA243650.019.592555
TCAACGC243800.019.5804964
ACATGGG106700.019.301223
AGTACTT118700.019.27068912-13
AACGCAG248800.019.2442156
AGAGTAC179950.018.93535610-11
GTACTTT120850.018.82967614-15
ATTCGCG2950.017.73028480-81
ACCGACG1350.00945820717.5750148
ACGCAGA272150.017.4012977
CGCAGAG272100.017.1603438
TACACCG3652.3264874E-916.9009045
TACTTTT140000.016.25404514-15
Produced by FastQC (version 0.11.5)