##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938887_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 611851 Sequences flagged as poor quality 0 Sequence length 101 %GC 40 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.989038180864295 33.0 31.0 34.0 30.0 34.0 2 32.23274947658825 34.0 31.0 34.0 30.0 34.0 3 32.30609413076059 34.0 31.0 34.0 30.0 34.0 4 35.85929417456211 37.0 35.0 37.0 35.0 37.0 5 35.797659887783134 37.0 35.0 37.0 35.0 37.0 6 35.881541421032246 37.0 35.0 37.0 35.0 37.0 7 35.83792132398247 37.0 35.0 37.0 35.0 37.0 8 35.840341847933566 37.0 35.0 37.0 35.0 37.0 9 37.42753219329543 39.0 37.0 39.0 34.0 39.0 10-11 37.329813140781006 39.0 37.0 39.0 34.0 39.0 12-13 37.280899271227796 39.0 37.0 39.0 34.0 39.0 14-15 38.37683194110984 40.0 38.0 41.0 33.5 41.0 16-17 38.53830916350549 40.0 38.0 41.0 34.0 41.0 18-19 38.600124049809516 40.0 38.0 41.0 34.0 41.0 20-21 38.56509591387446 40.0 38.0 41.0 34.0 41.0 22-23 38.472667365093784 40.0 38.0 41.0 34.0 41.0 24-25 38.330502851184356 40.0 37.5 41.0 33.5 41.0 26-27 38.08992793997231 40.0 37.0 41.0 33.5 41.0 28-29 37.96442924829738 40.0 37.0 41.0 33.0 41.0 30-31 37.82643568450489 40.0 37.0 41.0 33.0 41.0 32-33 37.61610261321792 40.0 36.0 41.0 33.0 41.0 34-35 37.42338821052838 40.0 36.0 41.0 32.5 41.0 36-37 37.221825248303915 39.5 35.5 41.0 31.5 41.0 38-39 37.04098301710711 39.0 35.0 41.0 31.0 41.0 40-41 37.0081874508663 39.0 35.0 41.0 31.0 41.0 42-43 36.78157590655242 39.0 35.0 41.0 31.0 41.0 44-45 36.52886650508049 39.0 35.0 41.0 30.0 41.0 46-47 36.17815530251646 39.0 35.0 41.0 30.0 41.0 48-49 35.77598059004561 39.0 35.0 40.0 28.0 41.0 50-51 35.53330385992668 39.0 35.0 40.0 28.0 41.0 52-53 35.410142338575895 38.5 35.0 40.0 27.0 41.0 54-55 35.19160302099694 38.0 34.5 40.0 27.0 41.0 56-57 35.03480994555864 38.0 34.5 40.0 26.5 41.0 58-59 34.791907670331504 38.0 34.0 40.0 26.0 41.0 60-61 34.29381172867251 38.0 33.5 40.0 25.0 41.0 62-63 33.94789417685024 37.0 33.0 40.0 24.0 41.0 64-65 33.56444297713006 37.0 33.0 39.5 22.5 40.5 66-67 33.027727338845565 36.0 32.0 39.0 22.0 40.0 68-69 32.45453631684838 35.5 31.5 38.5 21.0 40.0 70-71 31.788557998597696 35.0 31.0 37.5 19.0 39.5 72-73 31.195203570804004 35.0 30.5 37.0 16.5 39.0 74-75 30.685192146453957 34.0 30.0 36.5 12.5 39.0 76-77 28.786620435367432 32.0 27.5 34.5 8.0 36.5 78-79 29.772935731084857 34.0 29.0 35.0 7.5 37.0 80-81 29.69034863063066 34.0 29.0 35.0 6.0 37.0 82-83 29.21330274854499 34.0 29.0 35.0 2.0 36.5 84-85 28.703007758424846 33.5 29.0 35.0 2.0 36.0 86-87 28.10713392639711 33.0 27.5 35.0 2.0 35.5 88-89 27.310339445387847 33.0 26.0 35.0 2.0 35.0 90-91 26.644466544959474 32.0 25.0 34.0 2.0 35.0 92-93 26.2134702729913 32.0 25.0 34.0 2.0 35.0 94-95 25.574027009843903 32.0 23.5 34.0 2.0 35.0 96-97 24.537200233390156 31.0 17.5 34.0 2.0 35.0 98-99 23.287014322114373 31.0 2.0 34.0 2.0 35.0 100-101 17.646063338950167 23.5 2.0 30.5 2.0 32.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.14279436714571148 1101 2 0.15646393878257214 1101 3 0.19954781070513405 1101 4 0.11439201313193337 1101 5 0.15483738355887766 1101 6 0.3119218518527518 1101 7 0.2997890476826939 1101 8 0.25114329840544514 1101 9 0.24101955963382693 1101 10-11 0.38184028527660274 1101 12-13 0.3106036624740227 1101 14-15 0.28206967070595823 1101 16-17 0.27286150268331255 1101 18-19 0.26781922303761263 1101 20-21 0.36639448407837705 1101 22-23 0.4625936052658375 1101 24-25 0.43347066175165594 1101 26-27 0.2556702386755134 1101 28-29 0.37000388862035294 1101 30-31 0.40898109219376977 1101 32-33 0.3547575850283877 1101 34-35 0.1334127175164923 1101 36-37 0.23482133380766612 1101 38-39 0.14107500380688975 1101 40-41 0.18343886920074937 1101 42-43 0.36728882185561673 1101 44-45 0.42444389729830334 1101 46-47 0.3976843361639908 1101 48-49 0.3863197904369997 1101 50-51 0.1316123978844388 1101 52-53 -0.04192050621642096 1101 54-55 0.1813329474499028 1101 56-57 0.06348834070357157 1101 58-59 -0.04761555079650748 1101 60-61 0.04326784407329143 1101 62-63 0.0957643070598948 1101 64-65 0.18790400214374614 1101 66-67 -0.09818977471361023 1101 68-69 0.014809490515311552 1101 70-71 0.13071193607530773 1101 72-73 0.15453775975640482 1101 74-75 0.3212381710409389 1101 76-77 0.18751324116633938 1101 78-79 0.6155674849989126 1101 80-81 0.6431718682941394 1101 82-83 0.5073279206961026 1101 84-85 0.5856916642817716 1101 86-87 1.223914848133603 1101 88-89 1.043875292948215 1101 90-91 1.0503386311492982 1101 92-93 1.0104031354677439 1101 94-95 0.9008811645259769 1101 96-97 1.1354778710832747 1101 98-99 1.080933611640944 1101 100-101 1.658452145881185 1102 1 0.1551224862225027 1102 2 0.13861557934662727 1102 3 0.11978002422126366 1102 4 0.02848679710005797 1102 5 0.22799466654795708 1102 6 0.10564281341471116 1102 7 0.07616918609598855 1102 8 0.13354212793904452 1102 9 0.1901312144840901 1102 10-11 0.24602420138899816 1102 12-13 0.3088758460392569 1102 14-15 0.331040656548204 1102 16-17 0.4786298718940287 1102 18-19 0.3611197440455385 1102 20-21 0.386827519051387 1102 22-23 0.5166665839712365 1102 24-25 0.5453510836960263 1102 26-27 0.6116959789109231 1102 28-29 0.42429773959892003 1102 30-31 0.33143612667177536 1102 32-33 0.5339492238380004 1102 34-35 0.3002966013596051 1102 36-37 0.504307437652102 1102 38-39 0.6261152150923692 1102 40-41 0.5685633565734349 1102 42-43 0.4036434581369477 1102 44-45 0.43864238158190716 1102 46-47 0.4505558323621699 1102 48-49 0.424519728404789 1102 50-51 0.18646861160107875 1102 52-53 0.25434596913203933 1102 54-55 0.19525260006951584 1102 56-57 0.16270228604629722 1102 58-59 0.33373217735201877 1102 60-61 0.2962057586004221 1102 62-63 0.4665273972666455 1102 64-65 0.4066678066608205 1102 66-67 0.320369448818532 1102 68-69 0.38319311275792955 1102 70-71 0.2510794926013773 1102 72-73 0.3100177418359884 1102 74-75 0.5431834761736347 1102 76-77 0.09639167995965536 1102 78-79 0.3384965333057153 1102 80-81 0.7931535405948722 1102 82-83 0.8485489527492085 1102 84-85 0.7845409354032746 1102 86-87 0.9277141645260834 1102 88-89 0.856223149547283 1102 90-91 0.8609790354238775 1102 92-93 0.7285575831460811 1102 94-95 0.9224718208455833 1102 96-97 0.9431263176149542 1102 98-99 1.0852398377326935 1102 100-101 1.4615922916882162 1103 1 -0.04690940801185661 1103 2 -0.05889142800990044 1103 3 0.03081609257607454 1103 4 -0.0015037322064799241 1103 5 0.19211445009318595 1103 6 0.11270916247832474 1103 7 0.09776364271095872 1103 8 0.07119817913818594 1103 9 0.08081964142422748 1103 10-11 0.1627060147876378 1103 12-13 0.016982376991194315 1103 14-15 0.04852062300217597 1103 16-17 0.13267213605941208 1103 18-19 0.11787120052339617 1103 20-21 0.13024187942881582 1103 22-23 0.15113608164183034 1103 24-25 0.06618182614099766 1103 26-27 0.2632733117468007 1103 28-29 0.13938930557968376 1103 30-31 0.09191686800067345 1103 32-33 0.17051534809221636 1103 34-35 0.30392384506892256 1103 36-37 0.2922228965661944 1103 38-39 0.2715525411042421 1103 40-41 0.24639874507914072 1103 42-43 0.557904154121303 1103 44-45 0.5446710997857238 1103 46-47 0.4401013985151252 1103 48-49 0.4272493289456065 1103 50-51 0.3879472515202096 1103 52-53 0.28400486472799713 1103 54-55 0.4486845306560241 1103 56-57 0.6342854224721108 1103 58-59 0.40986124664114243 1103 60-61 0.580095375595377 1103 62-63 0.6664068611679994 1103 64-65 0.6312922474275311 1103 66-67 0.5400672157119217 1103 68-69 0.48172340143869974 1103 70-71 0.7758180918953812 1103 72-73 0.6602022130874907 1103 74-75 0.7374108174597964 1103 76-77 0.5227227588215158 1103 78-79 0.8985700667034635 1103 80-81 0.6380318724773133 1103 82-83 0.7591736677514902 1103 84-85 0.9342608005814164 1103 86-87 1.1787927712818416 1103 88-89 0.667467764485469 1103 90-91 0.417017160545452 1103 92-93 0.9373911572327351 1103 94-95 1.0517563530028973 1103 96-97 1.174954225229456 1103 98-99 1.6755669752554532 1103 100-101 1.7238938296395503 1104 1 0.1019008921614244 1104 2 0.24835297751283747 1104 3 0.15494423607294294 1104 4 0.08478444159963061 1104 5 0.036416250636015945 1104 6 0.0964645271519089 1104 7 0.05501705405332302 1104 8 0.03282407118015129 1104 9 0.12056946596601392 1104 10-11 0.1409523022161423 1104 12-13 0.13484837039521125 1104 14-15 0.026679957689488276 1104 16-17 0.19096929084150105 1104 18-19 0.2065583383497085 1104 20-21 0.11664619725350889 1104 22-23 0.09685893841203352 1104 24-25 0.15666702243680675 1104 26-27 0.21189045261645134 1104 28-29 0.04406736305544001 1104 30-31 0.12276490566362952 1104 32-33 0.250154742652704 1104 34-35 0.2480520506748931 1104 36-37 0.46929749580721847 1104 38-39 0.2810412589860789 1104 40-41 0.31463025054163296 1104 42-43 0.4054759988237109 1104 44-45 0.5307431339208861 1104 46-47 0.08254706857916005 1104 48-49 0.18494619926801192 1104 50-51 0.132411777907258 1104 52-53 0.229589528748825 1104 54-55 0.5834317776701354 1104 56-57 0.418252889725089 1104 58-59 0.464068517132489 1104 60-61 0.31748722812352526 1104 62-63 0.416863363059214 1104 64-65 0.4120812814446779 1104 66-67 0.15726553628183382 1104 68-69 0.2657126068769671 1104 70-71 0.17980363289670365 1104 72-73 0.3155075724818026 1104 74-75 0.04572971042718521 1104 76-77 0.1491582554148323 1104 78-79 0.6525372855200366 1104 80-81 0.6857068182871231 1104 82-83 0.5514295945124381 1104 84-85 0.5910676474886891 1104 86-87 0.7855769483673143 1104 88-89 0.6664352006838961 1104 90-91 0.8096382888193823 1104 92-93 0.8246677973788152 1104 94-95 1.2512430212843988 1104 96-97 1.1816557972108015 1104 98-99 1.3645106236682523 1104 100-101 0.7784628856433251 1105 1 -0.16946302177226258 1105 2 -0.0576326302077419 1105 3 -0.011578747271769885 1105 4 -0.0195955049980725 1105 5 -0.14224797341417883 1105 6 -0.09781328455109417 1105 7 0.14544175800581627 1105 8 0.14434627624019924 1105 9 0.06947208459455823 1105 10-11 0.07630378754247857 1105 12-13 0.009218259160377329 1105 14-15 -0.08679095242461443 1105 16-17 0.017381877500504572 1105 18-19 0.06632940292338674 1105 20-21 0.06919478017674408 1105 22-23 -0.1031272919362749 1105 24-25 -0.24908270516355913 1105 26-27 -0.2823741881153765 1105 28-29 -0.11880666259202854 1105 30-31 -0.10543414363172587 1105 32-33 -0.04327354140922779 1105 34-35 -0.09806909831274169 1105 36-37 0.021466515288281585 1105 38-39 -0.0812921581625261 1105 40-41 0.002077117646535953 1105 42-43 0.13167246181927084 1105 44-45 -0.20518188733226594 1105 46-47 -0.11593348029113315 1105 48-49 -0.1869750862187658 1105 50-51 -0.15616120723348814 1105 52-53 -0.20726165125045526 1105 54-55 -0.17069911783617187 1105 56-57 -0.08915578156338455 1105 58-59 -0.14110168773598275 1105 60-61 -0.32361578084470466 1105 62-63 0.11690946145834857 1105 64-65 0.04526674069250447 1105 66-67 0.02892639574756828 1105 68-69 -0.031466410568576464 1105 70-71 -0.012492546324295972 1105 72-73 0.314253336817206 1105 74-75 0.2352929131284327 1105 76-77 -0.14506028691786454 1105 78-79 0.052524713229363584 1105 80-81 -0.04751216706915429 1105 82-83 -0.06056556814905534 1105 84-85 0.13061947526028206 1105 86-87 0.30715339388139995 1105 88-89 0.30896306926154793 1105 90-91 0.05046351003182892 1105 92-93 0.13884934125610826 1105 94-95 0.21341539337630877 1105 96-97 0.5902702110427143 1105 98-99 0.7871221877809518 1105 100-101 0.7785826217449596 1106 1 -0.08048041217109514 1106 2 0.03756331550366809 1106 3 0.040818073166285274 1106 4 0.07405578452950579 1106 5 0.032280819671321126 1106 6 0.09112072912518698 1106 7 0.05260618578879672 1106 8 -0.08289173965761165 1106 9 -0.08287613991861775 1106 10-11 -0.03718505275048045 1106 12-13 0.02198336728631034 1106 14-15 -0.05387589115903779 1106 16-17 -0.2123466795476432 1106 18-19 -0.24888459270297858 1106 20-21 -0.06440062403721925 1106 22-23 -0.0836628884512578 1106 24-25 0.2217076728433085 1106 26-27 -0.04847158823339015 1106 28-29 -0.028713288993500896 1106 30-31 0.05705848020110693 1106 32-33 0.019198255598652736 1106 34-35 0.3379404820267382 1106 36-37 -0.06873967027872396 1106 38-39 0.19064543078537355 1106 40-41 0.25778713882047555 1106 42-43 0.24363400161585247 1106 44-45 0.3371052451610481 1106 46-47 0.2032723234507614 1106 48-49 0.1817551716867456 1106 50-51 0.3375907452813536 1106 52-53 0.2226560827909836 1106 54-55 0.4209562676336489 1106 56-57 0.35928047099657334 1106 58-59 0.4118418368570289 1106 60-61 0.6225295209706943 1106 62-63 0.6354106242894062 1106 64-65 0.29513657303656515 1106 66-67 0.368993735814918 1106 68-69 0.4426562594303789 1106 70-71 0.6035562578688456 1106 72-73 0.5485464396670103 1106 74-75 0.4195756025657609 1106 76-77 0.14606401451571216 1106 78-79 0.4391226513736939 1106 80-81 0.6190677211602171 1106 82-83 0.4345054166126481 1106 84-85 0.6361819730908209 1106 86-87 0.7525697568235366 1106 88-89 0.7520884814591255 1106 90-91 0.7926360949879161 1106 92-93 0.8888666969435697 1106 94-95 0.935099589287308 1106 96-97 0.4186658512940227 1106 98-99 0.4531466940116431 1106 100-101 0.9294295792929468 1107 1 -0.06259979379624525 1107 2 0.019613360156675697 1107 3 0.05254711537308765 1107 4 -0.12603130270749574 1107 5 -0.05716387438622661 1107 6 -0.1033500633686728 1107 7 -0.03956761149208177 1107 8 -0.03844951399627661 1107 9 -0.2460648640628591 1107 10-11 -0.17209122595978243 1107 12-13 -0.06106429772181343 1107 14-15 0.11217783667392922 1107 16-17 0.004206551832609762 1107 18-19 0.22669076549181 1107 20-21 0.03729036603927227 1107 22-23 0.06690928024282528 1107 24-25 -0.05684806576184798 1107 26-27 0.024936850908154895 1107 28-29 -0.0921885135066205 1107 30-31 0.03508143351236015 1107 32-33 0.01784757138141657 1107 34-35 -0.08382661231037503 1107 36-37 -0.022062020635672752 1107 38-39 -0.2541678863508423 1107 40-41 -0.14663323182724497 1107 42-43 -0.1375986988095974 1107 44-45 -0.1254916185890238 1107 46-47 -0.3307596492138387 1107 48-49 -0.3040612572927799 1107 50-51 -0.40132643309217286 1107 52-53 -0.3876188244361387 1107 54-55 -0.7047350219785713 1107 56-57 -0.3860697916518703 1107 58-59 -0.4076157816121224 1107 60-61 -0.39771548815404856 1107 62-63 -0.5150589256859135 1107 64-65 -0.43955382351544614 1107 66-67 -0.3583608090849353 1107 68-69 -0.5111861182175943 1107 70-71 -0.4135325322842007 1107 72-73 -0.21570924180448614 1107 74-75 -0.31748057147558484 1107 76-77 -0.5903930880863655 1107 78-79 -0.2433320438487101 1107 80-81 -0.4433508078975201 1107 82-83 -0.633903826837777 1107 84-85 -0.6383120056865188 1107 86-87 -0.15544504245979596 1107 88-89 0.14798179127335942 1107 90-91 -0.006405945405028035 1107 92-93 0.05102091346351756 1107 94-95 0.1171784781207279 1107 96-97 0.03757598503117521 1107 98-99 -0.0709964396866809 1107 100-101 0.26163826693820624 1108 1 0.22572598166416213 1108 2 0.07295156223212018 1108 3 0.06058620118184166 1108 4 0.12645260496756805 1108 5 0.13266611593289923 1108 6 0.14789118280285862 1108 7 0.12825962742162034 1108 8 0.2062019463432918 1108 9 0.07462672376981772 1108 10-11 0.1811147841060503 1108 12-13 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-2.080039031481455 2316 88-89 -1.9556961155659742 2316 90-91 -2.6731398074968453 2316 92-93 -2.55227467457755 2316 94-95 -2.625804813389248 2316 96-97 -2.2140963700757936 2316 98-99 -2.4869291701148555 2316 100-101 -2.1481287039615395 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 1.0 3 4.0 4 8.0 5 58.0 6 112.0 7 216.0 8 287.0 9 506.0 10 801.0 11 1133.0 12 1626.0 13 2241.0 14 2612.0 15 3541.0 16 4625.0 17 6319.0 18 6143.0 19 4428.0 20 4001.0 21 4565.0 22 5029.0 23 5509.0 24 6404.0 25 7406.0 26 8833.0 27 10581.0 28 12593.0 29 15216.0 30 18160.0 31 21943.0 32 27022.0 33 34563.0 34 47677.0 35 65741.0 36 90092.0 37 121816.0 38 67377.0 39 2662.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.904043511431595 22.768849621407885 16.30694263283538 18.020164234325136 2 14.1998623847963 26.279437313986577 36.229081917002674 23.29161838421446 3 23.208264757269333 26.500569583117457 33.11067563834986 17.18049002126335 4 11.20583279262435 19.46895567711747 42.32043422336484 27.004777306893345 5 9.865964099102559 41.5612624642274 36.29347667977988 12.279296756890158 6 24.578859885821874 40.81483890685804 17.15172484804307 17.454576359277013 7 22.07481886929988 36.48535346023787 23.850251123230983 17.58957654723127 8 22.53032192478234 30.596174558838673 21.573389599755497 25.30011391662349 9 20.72105790462057 22.110448458856812 27.135037778805625 30.033455857716994 10-11 23.40430921907458 26.53456478783233 28.658447890090887 21.402678103002202 12-13 27.74433644792605 26.249282913650546 27.509230188395538 18.497150450027867 14-15 21.836285066127967 29.031991814907066 31.05734468263152 18.074378436333443 16-17 17.275882442834178 31.42152708598756 31.31308343623358 19.98950703494468 18-19 16.947523688500812 33.549291285818775 31.3725233820519 18.130661643628518 20-21 19.934183322410195 28.58579948386127 29.548125278866916 21.931891914861627 22-23 18.11870863984859 28.861520206716996 31.546242467528856 21.473528685905556 24-25 21.99399853885995 29.532435184383125 29.913001694857083 18.56056458189984 26-27 20.282062136042924 26.48063008804431 35.41589373883511 17.821414037077655 28-29 16.97177907693213 32.92541811650222 31.856366991309976 18.246435815255673 30-31 18.86709345902842 28.791078220024158 30.80431346847517 21.537514852472253 32-33 19.366316309036023 29.347422820261798 32.470895691924994 18.815365178777185 34-35 21.903209065313245 27.937624891199075 31.23928847261895 18.91987757086873 36-37 20.030373323786495 27.257442526903603 34.35847387943196 18.353710269877936 38-39 18.837421981691595 29.97259705244645 32.85215402859289 18.33782693726907 40-41 18.414929844734125 30.795828521880974 31.867873227730637 18.921368405654263 42-43 17.67644532632262 30.914942250690764 29.400458137164925 22.008154285821693 44-45 19.710317369516968 27.647742391009444 30.379411678178354 22.262528561295237 46-47 22.103828329491137 30.53862365805803 30.238384954396242 17.119163058054593 48-49 20.24821990842325 29.15680904947645 31.17850929128942 19.41646175081088 50-51 15.846582811417214 30.41343601678848 34.4478530615447 19.292128110249603 52-53 19.038703867444852 32.5405204861968 28.590212322934832 19.830563323423515 54-55 17.536050443653767 28.84885372419102 30.767621528770896 22.84747430338432 56-57 20.82516821905987 28.38673141009821 29.628046697643708 21.160053673198213 58-59 23.38229705931625 27.993903709666217 29.366794830411173 19.25700440060636 60-61 19.580383577853524 30.137314301264457 32.546096271193086 17.736205849688933 62-63 18.054662313730137 30.017774036831074 32.03616914345486 19.891394505983925 64-65 17.7245819223075 33.030507577045285 29.88176802081563 19.36314247983159 66-67 19.604420462002512 29.232074255131884 27.9076191106611 23.2558861722045 68-69 17.82710456035748 29.468666542450777 30.44686665424508 22.257362242946662 70-71 21.540211587461652 29.57901515238187 28.622491423565542 20.258281836590935 72-73 20.060276112975217 26.692609802059653 33.149247120622505 20.097866964342625 74-75 17.420417716077935 33.14941055910671 29.138957033656887 20.29121469115847 76-77 18.880814012931616 28.708596948269445 29.363029973376086 23.047559065422853 78-79 19.078253265830647 29.090552484156074 30.82257662494739 21.008617625065888 80-81 21.021229859254834 28.31091908889508 29.466756993742756 21.20109405810733 82-83 19.11878872470585 27.95182160362572 32.28261455811954 20.646775113548888 84-85 18.43587608410878 29.357579997074453 30.752937196259545 21.453606722557225 86-87 18.160797887828515 30.35125974540089 29.857160150102892 21.6307822166677 88-89 18.25464888877217 29.606070329753297 28.21117801393005 23.92810276754448 90-91 19.278185694469624 26.84905751693713 29.71042608814774 24.162330700445505 92-93 20.818140364239007 29.455700816048353 29.917986568625366 19.808172251087274 94-95 19.488598057522506 27.89420566399307 30.372519296066418 22.244676982418003 96-97 16.28893308991895 28.552703190809524 32.51649502901851 22.64186869025302 98-99 18.702429186190756 29.64153037259071 28.222639171955265 23.433401269263268 100-101 17.98097407941005 26.88831158642771 30.435343985735052 24.695370348427186 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 8470.0 1 5092.5 2 1554.5 3 1206.5 4 889.0 5 865.5 6 1463.5 7 2669.5 8 3570.0 9 3531.0 10 2504.0 11 1504.0 12 1268.5 13 1194.5 14 1123.0 15 1035.0 16 1002.0 17 1011.0 18 1038.5 19 1071.0 20 1141.5 21 1344.5 22 1564.5 23 1789.5 24 2064.5 25 2721.5 26 4151.0 27 5696.5 28 6091.5 29 6592.5 30 8170.0 31 9861.0 32 11923.0 33 14646.0 34 17555.5 35 19341.5 36 21238.0 37 23336.0 38 22499.0 39 20060.5 40 18301.0 41 16902.0 42 15388.5 43 14667.5 44 14684.0 45 14795.5 46 17564.5 47 35598.0 48 68148.0 49 63610.0 50 29942.0 51 15053.0 52 11050.5 53 8062.0 54 5183.0 55 3760.5 56 3175.5 57 2792.0 58 2643.5 59 2349.5 60 2146.0 61 2013.0 62 1582.5 63 997.0 64 528.0 65 368.0 66 261.0 67 173.0 68 117.0 69 83.0 70 56.0 71 38.5 72 35.5 73 41.5 74 40.0 75 37.0 76 28.0 77 12.0 78 3.0 79 1.0 80 0.5 81 0.5 82 0.5 83 1.0 84 0.5 85 0.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.3850610687896236 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0011440693894428544 16-17 0.0020429810525765257 18-19 5.720346947214272E-4 20-21 0.0 22-23 0.0 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.011195536168119363 36-37 0.021247002946795872 38-39 0.009070835873439775 40-41 0.004821435284080602 42-43 0.004167681347256113 44-45 0.0014709463578550987 46-47 7.354731789275494E-4 48-49 0.00236985802098877 50-51 4.903154526183662E-4 52-53 0.0 54-55 0.0 56-57 0.0 58-59 5.720346947214272E-4 60-61 0.0010623501473397937 62-63 5.720346947214272E-4 64-65 4.903154526183662E-4 66-67 0.00236985802098877 68-69 0.0021247002946795874 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.09128039342911919 78-79 0.003840804378843869 80-81 8.171924210306104E-5 82-83 0.0 84-85 8.171924210306104E-5 86-87 0.08776646601868755 88-89 0.4237142703043715 90-91 0.3787686871476879 92-93 0.0 94-95 5.720346947214272E-4 96-97 0.0 98-99 0.0 100-101 2.451577263091831E-4 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 611851.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 29.07437799802094 #Duplication Level Percentage of deduplicated Percentage of total 1 77.11861638347686 22.421758034175767 2 11.450119905120067 6.658102284882489 3 3.7220791544794505 3.246514088276692 4 1.7595585712029231 2.046322840352457 5 1.0493836069094507 1.5255087826105989 6 0.6958580946772807 1.213898476657794 7 0.4877078907217284 0.9925862497222713 8 0.3785397452856616 0.8804646113367911 9 0.28966694475786403 0.7579697620879787 >10 2.498813644825535 14.933189614955698 >50 0.32540987514982417 6.5894283907199345 >100 0.1938970758664755 10.143791490331598 >500 0.0095543486658843 1.8126402107388837 >1k 0.0134884922341896 9.296541919189336 >5k 0.006182225607336901 11.5310911738413 >10k+ 0.0011240410195157999 5.95019207012041 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 23306 3.809097312907881 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 13108 2.1423516509738483 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 9108 1.48859771414936 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 6916 1.1303405567695404 No Hit ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC 6834 1.1169386010646383 No Hit GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA 6360 1.0394687595509364 No Hit TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC 6321 1.0330946586668976 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 6271 1.0249227344565914 No Hit GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT 6252 1.0218174032566754 No Hit GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG 6145 1.0043294854466203 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5651 0.9235908742487959 No Hit TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT 5555 0.9079007797650082 No Hit ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC 5155 0.8425253860825593 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4542 0.7423375952642064 No Hit GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA 4290 0.7011510972442637 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4052 0.6622527380032067 No Hit GATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATA 3852 0.6295650411619822 No Hit CCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCAC 3665 0.5990020446154374 No Hit AAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGC 3579 0.584946334973711 No Hit TGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGAT 3425 0.5597768084059681 No Hit GTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTG 3008 0.49162296049201526 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2750 0.4494558315668357 No Hit TACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACC 2478 0.4050005638627705 No Hit GCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAG 2327 0.38032135274764606 No Hit ATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATAC 2224 0.3634871888744155 No Hit ACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTG 2059 0.33651983898040533 No Hit CAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGT 1874 0.3062837194022728 No Hit GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 1837 0.30023649548664627 No Hit GCTTTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC 1564 0.2556177892983749 No Hit GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA 1507 0.24630179569862598 No Hit CTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCT 1255 0.2051152976786832 No Hit AACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACG 1236 0.2020099664787669 No Hit AGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAG 1220 0.19939495073146896 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1094 0.17880170172149756 No Hit GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT 1026 0.16768788479548127 No Hit ATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCA 1018 0.16638037692183227 No Hit CACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACT 1011 0.16523630753238944 No Hit AGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTA 987 0.1613137839114425 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 913 0.14921933608018945 No Hit TTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGA 852 0.139249588543616 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 734 0.11996384740729361 No Hit CTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTG 688 0.112445677133812 No Hit CAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGA 686 0.11211880016539974 No Hit CTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCA 626 0.10231249111303242 No Hit GTTATAGATTAACCCAATTTTAAGTTTAGGAAGTTGGTGTAAATTATGGA 619 0.10116842172358956 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 8.171924210306104E-5 0.0 18-19 0.0 0.0 0.0 3.2687696841224415E-4 0.0 20-21 0.0 0.0 0.0 5.720346947214273E-4 0.0 22-23 0.0 0.0 0.0 0.0016343848420612207 0.0 24-25 0.0 0.0 0.0 0.00310533119991632 0.0 26-27 0.0 0.0 0.0 0.0060472239156265165 0.0 28-29 0.0 0.0 0.0 0.021655599157311176 0.0 30-31 0.0 0.0 0.0 0.05573252311428763 0.0 32-33 0.0 0.0 0.0 0.12069932058622115 0.0 34-35 0.0 0.0 0.0 0.20674968252074444 0.0 36-37 0.0 0.0 0.0 0.3232813217597095 0.0 38-39 0.0 0.0 0.0 0.47854788175552543 0.0 40-41 0.0 0.0 0.0 0.6366746152249485 0.0 42-43 0.0 0.0 0.0 0.813923651346488 0.0 44-45 0.0 0.0 0.0 1.0366903053194323 0.0 46-47 0.0 0.0 0.0 1.2981101608071246 0.0 48-49 0.0 0.0 0.0 1.5239004267378822 0.0 50-51 0.0 0.0 0.0 1.7679140836576224 0.0 52-53 0.0 0.0 0.0 2.009803040282683 0.0 54-55 0.0 0.0 0.0 2.2751454193913223 0.0 56-57 0.0 0.0 0.0 2.6689504470859733 0.0 58-59 0.0 0.0 0.0 3.010455159834666 0.0 60-61 0.0 0.0 0.0 3.4095719382660157 0.0 62-63 0.0 0.0 0.0 3.7922631490346506 0.0 64-65 0.0 0.0 0.0 4.197590589865833 0.0 66-67 0.0 0.0 0.0 4.686761973094757 0.0 68-69 0.0 0.0 0.0 5.251850532237423 0.0 70-71 0.0 0.0 0.0 5.845295668389853 0.0 72-73 0.0 0.0 0.0 6.396328517890794 0.0 74-75 0.0 0.0 0.0 7.079583101114487 0.0 76-77 0.0 0.0 0.0 7.680464688298295 0.0 78-79 0.0 0.0 0.0 8.558456225453583 0.0 80-81 0.0 0.0 0.0 9.438981059114067 0.0 82-83 0.0 0.0 0.0 10.194884048567381 0.0 84-85 0.0 0.0 0.0 11.041822273723504 0.0 86-87 0.0 0.0 0.0 11.89145723386903 0.0 88-89 0.0 0.0 0.0 12.811615899949498 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GACAGGT 50 2.8408229E-5 47.45636 7 GTAAGCG 225 0.0 43.24155 94-95 CTCCGAC 90 5.456968E-12 31.640158 94-95 CGCAGAA 180 5.456968E-12 31.637571 9 ATAGGGT 115 3.888412E-6 28.88648 3 ATACAGT 85 6.438235E-4 27.915508 6 ATCACCG 55 9.115465E-5 26.05777 84-85 CATGGGT 165 8.471761E-8 25.885288 4 CATTCCG 110 9.5288844E-5 25.885288 9 CTTTGCG 555 0.0 25.652088 2 ACCGCAG 75 1.0057174E-6 25.487097 86-87 GATCCTC 95 0.001232485 24.977032 5 TTGCGTT 610 0.0 24.89514 4 GCTTTGC 585 0.0 24.418407 1 GCAGAGA 890 0.0 24.26338 94-95 TTTGCGT 590 0.0 24.130354 3 GAGTACT 3065 0.0 23.846256 12-13 GTGTATA 120 1.681518E-4 23.80795 1 CGTCTTA 50 0.0016617866 23.73012 14-15 TTACTCG 50 0.0016617866 23.73012 94-95 >>END_MODULE