##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938886_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 615775 Sequences flagged as poor quality 0 Sequence length 101 %GC 40 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.976881166010312 33.0 31.0 34.0 30.0 34.0 2 32.02836100848524 33.0 31.0 34.0 30.0 34.0 3 32.07599204254801 34.0 31.0 34.0 30.0 34.0 4 35.576913645406194 37.0 35.0 37.0 33.0 37.0 5 35.62761885428931 37.0 35.0 37.0 33.0 37.0 6 35.7655637205148 37.0 35.0 37.0 35.0 37.0 7 35.7302927205554 37.0 35.0 37.0 35.0 37.0 8 35.72516909585482 37.0 35.0 37.0 35.0 37.0 9 37.320638220129105 39.0 37.0 39.0 34.0 39.0 10-11 37.23453452965775 39.0 37.0 39.0 34.0 39.0 12-13 37.17949250943933 39.0 37.0 39.0 34.0 39.0 14-15 38.291455482928015 40.0 38.0 41.0 34.0 41.0 16-17 38.27259956964801 40.0 38.0 41.0 34.0 41.0 18-19 38.25358044740368 40.0 38.0 41.0 34.0 41.0 20-21 38.12269984978279 40.0 38.0 41.0 34.0 41.0 22-23 38.22365555600666 40.0 38.0 41.0 34.0 41.0 24-25 38.11512890260241 40.0 38.0 41.0 33.5 41.0 26-27 37.980541593926354 40.0 37.5 41.0 33.5 41.0 28-29 37.912390889529455 40.0 37.5 41.0 33.5 41.0 30-31 37.79945272217937 40.0 37.0 41.0 33.0 41.0 32-33 37.562445698510004 40.0 36.5 41.0 33.0 41.0 34-35 37.1994169948439 40.0 36.0 41.0 32.5 41.0 36-37 36.88192765214567 39.5 35.5 41.0 31.5 41.0 38-39 36.6759952904876 39.0 35.0 41.0 31.0 41.0 40-41 36.69733993747717 39.0 35.0 41.0 31.0 41.0 42-43 36.47289838009013 39.0 35.0 41.0 30.5 41.0 44-45 36.354182128212415 39.0 35.0 41.0 30.0 41.0 46-47 36.07745523933255 39.0 35.0 41.0 29.5 41.0 48-49 35.82296374487434 39.0 35.0 41.0 28.0 41.0 50-51 34.62997361049084 38.0 34.0 40.0 25.5 40.5 52-53 34.91147497056555 38.5 34.5 39.5 27.5 40.5 54-55 35.511651171288214 39.0 35.0 40.5 27.5 41.0 56-57 35.399829483171615 39.0 35.0 41.0 27.0 41.0 58-59 35.26380577321262 39.0 35.0 41.0 26.5 41.0 60-61 34.89588892046608 38.5 35.0 40.0 26.0 41.0 62-63 34.65208720717794 38.0 35.0 40.0 26.0 41.0 64-65 34.27126223052251 37.5 34.0 40.0 25.5 41.0 66-67 33.758886768706105 37.0 34.0 39.0 22.5 41.0 68-69 33.32344362794852 36.5 33.5 39.0 22.0 41.0 70-71 32.7379692257724 36.0 33.0 38.5 21.5 40.0 72-73 32.25417157240875 35.0 32.5 37.5 19.5 40.0 74-75 31.809850188786488 35.0 32.0 37.0 18.0 39.0 76-77 31.40691486338354 35.0 32.0 36.5 14.0 39.0 78-79 30.917438999634605 35.0 31.0 36.0 7.5 38.5 80-81 30.40726564085908 35.0 31.0 36.0 4.5 37.5 82-83 29.973199626486945 34.0 30.5 35.0 2.0 37.0 84-85 29.579807559579393 34.0 30.0 35.0 2.0 36.5 86-87 29.325297389468556 34.0 30.0 35.0 2.0 36.0 88-89 28.822088425155293 34.0 29.0 35.0 2.0 36.0 90-91 28.327153586943282 34.0 29.0 35.0 2.0 35.0 92-93 27.903483415208477 34.0 28.5 35.0 2.0 35.0 94-95 27.351672282895535 34.0 27.0 35.0 2.0 35.0 96-97 26.724897080914296 34.0 25.0 35.0 2.0 35.0 98-99 26.144356298972838 33.5 24.5 35.0 2.0 35.0 100-101 23.553197190532256 30.5 10.5 34.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.10626461453056635 1101 2 0.3208787210549957 1101 3 0.37982755417433367 1101 4 0.29718071313380534 1101 5 0.2784528863202169 1101 6 0.3159508604322525 1101 7 0.18516908183404723 1101 8 0.2895388542703472 1101 9 0.23038700591423833 1101 10-11 0.47156896686067995 1101 12-13 0.4485251139660207 1101 14-15 0.4307214078493331 1101 16-17 0.5285987160338479 1101 18-19 0.4388920869299895 1101 20-21 0.5172131083944649 1101 22-23 0.4117874545899056 1101 24-25 0.6518135517254109 1101 26-27 0.5103813706912987 1101 28-29 0.4487952003152813 1101 30-31 0.36477978986057025 1101 32-33 0.6502329809050593 1101 34-35 0.6325710506409976 1101 36-37 0.3074359923150567 1101 38-39 0.2773107564206896 1101 40-41 0.20641660346516488 1101 42-43 0.228652566200239 1101 44-45 0.40352445102277557 1101 46-47 0.5243661667749606 1101 48-49 0.6268818321980447 1101 50-51 0.2624931250141387 1101 52-53 0.4675626023653905 1101 54-55 0.42772641218883223 1101 56-57 0.423840031434203 1101 58-59 0.44948898328264875 1101 60-61 0.3987911624544864 1101 62-63 0.3620263702828552 1101 64-65 0.339934643987327 1101 66-67 0.3200718067420425 1101 68-69 0.39933155398478704 1101 70-71 0.4002879138105939 1101 72-73 -0.05223118770754809 1101 74-75 -0.18229436165472634 1101 76-77 -0.1829235612237774 1101 78-79 -0.14670906101777703 1101 80-81 -0.14200908319775607 1101 82-83 -0.23170044989774752 1101 84-85 -0.4075521258444539 1101 86-87 -0.5737354818251674 1101 88-89 -0.24647094015476156 1101 90-91 -0.2289940472362808 1101 92-93 -0.07300858700733315 1101 94-95 -0.22033696720150786 1101 96-97 -0.24680764057364613 1101 98-99 -0.2518816273858455 1101 100-101 0.4895095952603761 1102 1 0.3994482552683394 1102 2 0.11456202251696368 1102 3 0.1281805243797507 1102 4 0.2628768453896768 1102 5 0.3639966528984715 1102 6 0.3687020787408102 1102 7 0.33763037354954406 1102 8 0.26895406616350215 1102 9 0.24203033451827594 1102 10-11 0.19366507444195236 1102 12-13 0.22455990515718582 1102 14-15 0.5095721881836681 1102 16-17 0.3803768296603991 1102 18-19 0.5260643773500746 1102 20-21 0.5413818826835239 1102 22-23 0.5166468093490266 1102 24-25 0.462516161064741 1102 26-27 0.4503156745590431 1102 28-29 0.4453762255450684 1102 30-31 0.5898754039497689 1102 32-33 0.5296790360527979 1102 34-35 0.3654360805589505 1102 36-37 0.3839843236949818 1102 38-39 0.7476241238872348 1102 40-41 0.6352581308231322 1102 42-43 0.3182290883148511 1102 44-45 0.4492927564943727 1102 46-47 0.5548207963474638 1102 48-49 0.7678200840023734 1102 50-51 0.6644686354700013 1102 52-53 0.6633864256952222 1102 54-55 0.6122215081113467 1102 56-57 0.5919403953846043 1102 58-59 0.4438693422981288 1102 60-61 0.556862842178127 1102 62-63 0.5363339392175277 1102 64-65 0.595797946683021 1102 66-67 0.6553749959345581 1102 68-69 0.7572983666428641 1102 70-71 0.5650710857308994 1102 72-73 0.5337752276894037 1102 74-75 0.5699340634886987 1102 76-77 0.5932639040775634 1102 78-79 0.370015693778349 1102 80-81 0.5370761058713924 1102 82-83 0.37769903193557397 1102 84-85 0.5410337109330428 1102 86-87 0.592819635070768 1102 88-89 0.5797180673093045 1102 90-91 0.7510842946303562 1102 92-93 0.6045868728655925 1102 94-95 0.48257155170540855 1102 96-97 0.46344003076063345 1102 98-99 0.4034573399819905 1102 100-101 0.9036743599263453 1103 1 0.05979308285454721 1103 2 0.10421719493075443 1103 3 0.06955983472457916 1103 4 0.11632512125174799 1103 5 -0.11358955399808224 1103 6 -0.04853930056953715 1103 7 0.19625106320471986 1103 8 -0.14139076142270568 1103 9 -0.12176276892999738 1103 10-11 0.024699557200570155 1103 12-13 0.15645645688132248 1103 14-15 0.20181356749401402 1103 16-17 0.17606648483280907 1103 18-19 0.3286505842466241 1103 20-21 0.1956922275111097 1103 22-23 0.11147439555592342 1103 24-25 0.20992995416818871 1103 26-27 0.021867398696969076 1103 28-29 0.1333072600278271 1103 30-31 0.2752202315359753 1103 32-33 0.5365755877769374 1103 34-35 0.15853952883481526 1103 36-37 0.2357084616260252 1103 38-39 0.36055515836999064 1103 40-41 0.17577537220244466 1103 42-43 0.057884260728641834 1103 44-45 0.20618930821850512 1103 46-47 0.14706217565781543 1103 48-49 0.34626835986444604 1103 50-51 -0.14242791625414242 1103 52-53 0.014248494660733968 1103 54-55 0.24153185293894097 1103 56-57 0.16780246435012458 1103 58-59 0.08093830781537292 1103 60-61 0.24134560079881595 1103 62-63 0.11564428404511062 1103 64-65 0.11821173978646726 1103 66-67 0.5484784442104242 1103 68-69 0.17367767698768688 1103 70-71 -0.0452737418553113 1103 72-73 0.018257986310096896 1103 74-75 -0.11885903995958103 1103 76-77 -0.055011957991403904 1103 78-79 0.11742948688179666 1103 80-81 0.014662312767946162 1103 82-83 0.30614730779764443 1103 84-85 0.03672336610545557 1103 86-87 -0.24597346837751033 1103 88-89 -0.26683365682862714 1103 90-91 -0.3532260501972324 1103 92-93 -0.5764476098930267 1103 94-95 -0.4726008620876989 1103 96-97 -0.42190479682557225 1103 98-99 -0.6999909358800771 1103 100-101 -0.045463571108140144 1104 1 0.20502218063442257 1104 2 0.27785914108096677 1104 3 0.10097221072835794 1104 4 0.2569486451062559 1104 5 0.1704444563939802 1104 6 0.21858398710169524 1104 7 0.2719832313672157 1104 8 0.2739257241701196 1104 9 0.1865863099552172 1104 10-11 0.26151462569372086 1104 12-13 0.18303298026346937 1104 14-15 0.17153487122571676 1104 16-17 0.08631093452577687 1104 18-19 0.25502044726032125 1104 20-21 0.44231480663250977 1104 22-23 0.21678261473400795 1104 24-25 0.1920685937525093 1104 26-27 0.2083222876908266 1104 28-29 0.2724192109017025 1104 30-31 0.37814299960399467 1104 32-33 0.3191393572620598 1104 34-35 0.32545615613759793 1104 36-37 0.347712476741755 1104 38-39 0.49192620230007833 1104 40-41 0.5301447628495808 1104 42-43 0.4462699480219854 1104 44-45 0.5472084390640433 1104 46-47 0.6127624590777501 1104 48-49 0.6205302871199905 1104 50-51 0.6209931985309325 1104 52-53 0.40827896230362626 1104 54-55 0.33030133805939244 1104 56-57 0.5539326013364274 1104 58-59 0.6194184684199939 1104 60-61 0.4687960023103912 1104 62-63 0.3019692722359508 1104 64-65 0.2659798078072555 1104 66-67 0.2916468428877934 1104 68-69 0.31714130476284197 1104 70-71 0.6354359888957504 1104 72-73 0.5108064038821212 1104 74-75 0.5265944319346083 1104 76-77 0.6184233277903566 1104 78-79 0.7640874934949302 1104 80-81 0.7186183826781942 1104 82-83 0.6181277045855502 1104 84-85 0.46463432500956614 1104 86-87 0.9604743351180005 1104 88-89 1.056182403634292 1104 90-91 1.0628875539595946 1104 92-93 1.0392821964366838 1104 94-95 1.0261709848655407 1104 96-97 1.1246528318943128 1104 98-99 0.670037406113309 1104 100-101 0.8608720453302183 1105 1 -0.30272502783800803 1105 2 -0.10456286881888133 1105 3 -0.2908719241858826 1105 4 -0.1354135052333305 1105 5 -0.23117865860544384 1105 6 -0.2691275358636531 1105 7 -0.3636591077602205 1105 8 -0.3703099152911449 1105 9 -0.39313338614240223 1105 10-11 -0.5206785940599303 1105 12-13 -0.23017785201464847 1105 14-15 -0.17580886078764024 1105 16-17 -0.1494623473897292 1105 18-19 -0.04088723087942725 1105 20-21 -0.05056248126894758 1105 22-23 -0.07276930192306708 1105 24-25 -0.044467321983646 1105 26-27 -0.1653826737458033 1105 28-29 -0.18512942499101115 1105 30-31 0.1391292434165905 1105 32-33 -0.12276952958035281 1105 34-35 -0.23591859924400893 1105 36-37 -0.3016212978639814 1105 38-39 -0.4479278900217807 1105 40-41 -0.36927678659210983 1105 42-43 -0.516376823015193 1105 44-45 -0.8396814538794359 1105 46-47 -0.7854863609982274 1105 48-49 -0.7547675717495821 1105 50-51 -0.661936754272098 1105 52-53 -0.5917570109897952 1105 54-55 -0.7556863446849391 1105 56-57 -0.6025756869103773 1105 58-59 -0.5959162271020446 1105 60-61 -0.4048873751501816 1105 62-63 -0.5010755073197046 1105 64-65 -0.45050747203618613 1105 66-67 -0.3785571976325173 1105 68-69 -0.47306964262981666 1105 70-71 -0.8000984303513974 1105 72-73 -0.46253019102980986 1105 74-75 -0.34987903416415733 1105 76-77 -0.3683341834333085 1105 78-79 -0.4108376820547406 1105 80-81 -0.6073313122682755 1105 82-83 -0.6491265258737542 1105 84-85 -0.48302453305420556 1105 86-87 -0.440308443746968 1105 88-89 -0.2816989132760348 1105 90-91 -0.4019003475023588 1105 92-93 -0.30972781852820574 1105 94-95 -0.2682977615371307 1105 96-97 -0.3629363818733857 1105 98-99 -0.2682899796355116 1105 100-101 0.15075491628682158 1106 1 0.05975298662358597 1106 2 -0.0689756106598054 1106 3 -0.012837919886486304 1106 4 0.04686126513993827 1106 5 -0.031667226125470904 1106 6 0.113420832320827 1106 7 -0.001005208991408324 1106 8 0.05212510522868996 1106 9 0.10401337954061773 1106 10-11 0.13915138444310315 1106 12-13 0.11885478429568508 1106 14-15 0.11376510833488851 1106 16-17 0.32466311674941295 1106 18-19 0.33238812577486243 1106 20-21 0.31097748355418986 1106 22-23 0.12102589057253255 1106 24-25 0.3338989082293722 1106 26-27 0.12717155724892137 1106 28-29 0.20569527698280154 1106 30-31 0.3114958644836179 1106 32-33 0.35193244423243186 1106 34-35 0.27006146720118096 1106 36-37 -0.029645530812977938 1106 38-39 -0.02488704577710621 1106 40-41 -0.10498130095599123 1106 42-43 -0.09577881637068231 1106 44-45 0.4007133086767496 1106 46-47 0.2672105317123439 1106 48-49 0.3029615664173093 1106 50-51 0.41090866296455886 1106 52-53 0.21960706952613407 1106 54-55 -0.07885421648482094 1106 56-57 -0.11168258548376286 1106 58-59 0.11266301854970351 1106 60-61 0.32576535105658166 1106 62-63 0.23686664628591814 1106 64-65 0.05779817580434354 1106 66-67 0.13370584712026812 1106 68-69 0.0130550398012943 1106 70-71 -0.15127099239947484 1106 72-73 -0.11452354651154195 1106 74-75 0.15223157752237526 1106 76-77 -0.08146687837174582 1106 78-79 -0.13163111570727182 1106 80-81 -0.178681712893642 1106 82-83 0.1588566674035583 1106 84-85 -0.0318812850573309 1106 86-87 0.09391082535601925 1106 88-89 -0.16213666977398233 1106 90-91 -0.23519133831729278 1106 92-93 -0.16992910834646224 1106 94-95 -0.1560640308355481 1106 96-97 -0.28271359508424965 1106 98-99 -0.22153406796851982 1106 100-101 0.2409151664333926 1107 1 -0.15030208250996324 1107 2 -0.23826650359476886 1107 3 0.004574227017357657 1107 4 -0.05238485936271786 1107 5 -0.04496475225926133 1107 6 -0.12903333804824513 1107 7 -0.2136326235719963 1107 8 0.009722438365820096 1107 9 0.03221126619894221 1107 10-11 0.07308115856343278 1107 12-13 0.07197810406454863 1107 14-15 0.15016139102688442 1107 16-17 0.03576806504781871 1107 18-19 0.07162332700642082 1107 20-21 0.049053855896239895 1107 22-23 -0.028431907680875668 1107 24-25 -0.04220686072929425 1107 26-27 0.15441747800144157 1107 28-29 0.17171852713703117 1107 30-31 0.21442307474809752 1107 32-33 0.18505117962325812 1107 34-35 -0.06064481000088051 1107 36-37 0.013337257960394311 1107 38-39 0.1101483558087466 1107 40-41 0.18734354467498093 1107 42-43 0.31372782531772714 1107 44-45 0.2571204014453201 1107 46-47 0.26834955156100904 1107 48-49 0.1879134870453143 1107 50-51 0.23272775548409896 1107 52-53 0.03503067171531882 1107 54-55 -0.20314887313755037 1107 56-57 0.06580076077261054 1107 58-59 -0.1118611399905376 1107 60-61 0.2666084804322608 1107 62-63 0.22113685292251972 1107 64-65 0.31585904049727276 1107 66-67 0.07385000034036437 1107 68-69 0.05377959791820075 1107 70-71 0.1903486513773771 1107 72-73 0.028708553190192276 1107 74-75 -0.07685997986441251 1107 76-77 -0.227071818180562 1107 78-79 -0.3538064879757492 1107 80-81 -0.458603269091892 1107 82-83 -0.23606910529052882 1107 84-85 -0.11242484513815398 1107 86-87 0.042339402444319774 1107 88-89 0.048962618798466195 1107 90-91 -0.18624461331771514 1107 92-93 -0.04233200159837125 1107 94-95 -0.008384801463837022 1107 96-97 0.08696555505130021 1107 98-99 -0.22440496395973497 1107 100-101 -0.3305572678713382 1108 1 0.13650524760549843 1108 2 0.12781633286179073 1108 3 -0.10464993539036627 1108 4 0.26020682623259006 1108 5 0.16787596324329712 1108 6 0.11986347720824142 1108 7 0.10210594826219221 1108 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-2.078776689629251 2316 74-75 -2.1403552246403486 2316 76-77 -2.203348189035548 2316 78-79 -1.6490729761723912 2316 80-81 -1.9115329929405434 2316 82-83 -1.9552889908597493 2316 84-85 -2.0957696310934217 2316 86-87 -2.050672589405231 2316 88-89 -2.084079865656985 2316 90-91 -2.3870544091270105 2316 92-93 -2.486618574830729 2316 94-95 -2.4306226915091216 2316 96-97 -2.8258215359350096 2316 98-99 -3.5974216322965766 2316 100-101 -3.7537268764302674 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 2908.0 3 542.0 4 439.0 5 419.0 6 472.0 7 528.0 8 614.0 9 771.0 10 934.0 11 1406.0 12 1988.0 13 3366.0 14 3355.0 15 3180.0 16 4400.0 17 6074.0 18 5085.0 19 3160.0 20 2931.0 21 3143.0 22 3487.0 23 4061.0 24 4673.0 25 5458.0 26 6444.0 27 7848.0 28 9287.0 29 11141.0 30 13443.0 31 16667.0 32 21086.0 33 27503.0 34 38074.0 35 61574.0 36 79520.0 37 120030.0 38 119947.0 39 19810.0 40 7.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.124439348683595 21.7638570856229 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33.340269519606785 31.617467159461025 18.091253658770523 20-21 19.928566435328563 28.517989260579697 29.649318747490316 21.90412555660143 22-23 18.070331185481102 28.579685968070006 31.856502008835296 21.49348083761359 24-25 22.07530018603078 29.134794260365037 30.09730840781069 18.692597145793492 26-27 20.307443628830036 26.24662163260925 35.658078322833695 17.787856415727013 28-29 17.106613181484846 32.64232949972276 32.02907647004304 18.221980848749347 30-31 18.690974127593847 28.59850009145784 31.114444241204804 21.596081539743512 32-33 19.280785141938324 29.226019329397218 32.64260287591645 18.850592652748006 34-35 21.82910547396529 27.891645515143516 31.235134839941246 19.044114170949953 36-37 20.02067919560216 26.89201980694517 34.64737152886389 18.439929468588776 38-39 18.898552760191638 29.8295698130094 32.944691643931485 18.327185782867485 40-41 18.5044386849248 30.53888696149058 32.178282075876005 18.77839227770862 42-43 17.5571989284055 30.77249682987988 29.475789100665708 22.194515141048914 44-45 19.572576212802133 27.59283592718328 30.5224422396425 22.312145620372085 46-47 22.17011522786308 30.294042953837813 30.404199843706238 17.131641974592874 48-49 20.122643466556458 29.03365181212746 31.332318382961905 19.511386338354182 50-51 15.884673777846018 30.220334401289715 34.74405389491015 19.150937925954118 52-53 18.999130211076945 32.332871896017345 29.00276454037985 19.66523335252586 54-55 17.420461226889657 28.53163585287777 31.291647612203565 22.756255308029004 56-57 20.71591333079151 28.20545687154496 29.85979404673749 21.218835750926036 58-59 23.308133791605776 27.756614879703484 29.67172562434178 19.263525704348964 60-61 19.795789140414755 29.350353896946384 33.204378954514816 17.649478008124046 62-63 18.172034406228004 29.56440225956223 32.54195851590072 19.721604818309054 64-65 17.895378251817196 32.550640092070665 30.347788939921276 19.206192716190863 66-67 19.57184146076466 28.918101567975846 28.423626326048137 23.086430645211358 68-69 17.891648891891517 29.00393999573569 30.978185837418604 22.12622527495419 70-71 21.651824918289375 29.076892537354887 29.116757356407003 20.154525187948735 72-73 20.181172146211967 26.228548090667758 33.67686338574637 19.913416377373903 74-75 17.663125824460806 32.762306162372326 29.771662643910528 19.80290536925634 76-77 19.026078179002866 28.463860341436963 29.783857858734216 22.726203620825956 78-79 19.3989537294312 28.698138611662475 31.33610564279013 20.566802016116192 80-81 21.275212938287087 28.17839786800439 29.894477974604172 20.651911219104353 82-83 19.37620138719903 27.60344790981083 32.858113415851044 20.162237287139092 84-85 18.82868867520059 29.164941507931648 31.25889630673118 20.747473510136583 86-87 18.672522074140367 30.028833363410616 30.581901532144997 20.71674303030402 88-89 18.729430480536642 29.326928573645212 28.709373412625173 23.23426753319298 90-91 19.82171240384411 26.548581491349445 30.2706658585951 23.35904024621135 92-93 21.491994046792474 29.014371670312546 30.59898024365021 18.894654039244774 94-95 20.089411522536263 27.69494546354557 30.864619061660953 21.35102395225722 96-97 17.098175158931316 28.10799242978066 33.128367820523394 21.66546459076463 98-99 19.62583308623581 29.461866521593873 28.87234492690406 22.039955465266257 100-101 18.897637152234754 26.628076671482248 30.843195205868206 23.631090970414792 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 10126.0 1 5917.0 2 1584.5 3 1262.5 4 907.5 5 914.5 6 1519.0 7 2697.0 8 3682.0 9 3648.0 10 2616.0 11 1642.5 12 1404.5 13 1347.0 14 1288.5 15 1224.5 16 1325.0 17 1426.5 18 1272.0 19 1198.0 20 1286.0 21 1445.0 22 1677.0 23 1903.0 24 2146.5 25 2781.0 26 4231.0 27 5724.0 28 6142.5 29 6697.5 30 8206.0 31 9819.0 32 11727.0 33 14417.0 34 17707.0 35 19470.5 36 21070.0 37 23374.5 38 22389.0 39 19869.0 40 18161.0 41 16767.0 42 15402.0 43 14663.5 44 14745.5 45 14706.0 46 18122.5 47 35142.5 48 68185.0 49 65640.5 50 31465.0 51 14477.0 52 8260.0 53 6055.0 54 4813.0 55 4002.5 56 3400.0 57 2921.0 58 2659.5 59 2406.5 60 2247.0 61 2082.5 62 1665.5 63 1099.5 64 649.5 65 482.5 66 354.5 67 246.0 68 177.0 69 137.0 70 106.0 71 91.5 72 81.5 73 78.0 74 70.0 75 56.5 76 43.5 77 31.0 78 18.5 79 10.0 80 6.5 81 5.5 82 5.0 83 4.5 84 3.0 85 2.0 86 1.5 87 0.5 88 0.0 89 0.5 90 1.0 91 0.5 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.28533149283423326 2 0.08931833867890057 3 0.09321586618488896 4 0.10896837318825871 5 0.11172912183833379 6 0.08655759002882547 7 0.0966262027526288 8 0.09272867524664041 9 0.09792537858795826 10-11 0.09142949941131095 12-13 0.09532702691729933 14-15 0.10417766229548131 16-17 0.0929722707157647 18-19 0.10450245625431367 20-21 0.10685721245584831 22-23 0.12049855872680769 24-25 0.13365271405951848 26-27 0.13527668385368033 28-29 0.1260200560269579 30-31 0.11976777231943486 32-33 0.11392148106045227 34-35 0.3806585197515326 36-37 0.5790264301083999 38-39 0.5637611140432788 40-41 0.5298201453452966 42-43 0.5548292801753887 44-45 0.5725305509317526 46-47 0.5629491291461979 48-49 0.5836547440217612 50-51 0.5759408874994926 52-53 0.5777272542730705 54-55 0.5675774430595592 56-57 0.5447606674515855 58-59 0.540781941455889 60-61 0.4914132597133693 62-63 0.5592951971093337 64-65 0.590556615646949 66-67 0.6028175875928707 68-69 0.603873167959076 70-71 0.6019244042060817 72-73 0.5927489748690675 74-75 0.5899070277292843 76-77 0.5747229101538711 78-79 0.5536925013194754 80-81 0.5506881572002761 82-83 0.5622183427388251 84-85 0.5704193901993423 86-87 0.5624619382079493 88-89 0.571962161503796 90-91 0.5875522715277496 92-93 0.5956721204985588 94-95 0.5946165401323535 96-97 0.5910438065851975 98-99 0.5957533189882668 100-101 0.5726929479111689 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 615775.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 29.924807969809823 #Duplication Level Percentage of deduplicated Percentage of total 1 78.18794491910907 23.397592372584068 2 10.841323861447705 6.488490693846794 3 3.5987946190173057 3.230797136906334 4 1.6595821176583136 1.9865070472422153 5 1.002472295785229 1.4999395473213688 6 0.6627707280306733 1.1899972058597372 7 0.4585858302392733 0.9606165035310242 8 0.3829807242678263 0.9168499703882711 9 0.3090613120682493 0.8323740373085846 >10 2.367175400746168 14.567782023962591 >50 0.31780296160699234 6.6011829138329645 >100 0.18261405169526987 9.781143368580278 >500 0.00981209830004435 1.9989348216104252 >1k 0.01253768116116778 9.852592156883311 >5k 0.005451165722246862 10.705899489333461 >10k+ 0.0010902331444493723 5.989300710808584 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 22474 3.6497097153992937 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 14242 2.3128577808452766 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 9833 1.596849498599326 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 7469 1.2129430392594698 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 6574 1.067597742681986 No Hit GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT 6515 1.0580163208964313 No Hit ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC 6487 1.0534692054727781 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5994 0.9734074946206002 No Hit GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG 5878 0.9545694450083229 No Hit TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC 5869 0.9531078721935772 No Hit GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA 5708 0.9269619585075717 No Hit TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT 5303 0.8611911818440177 No Hit ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC 4994 0.8110105152044171 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4911 0.797531565912874 No Hit GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA 4146 0.6732978766594941 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4109 0.6672891884210954 No Hit CCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCAC 3951 0.6416304656733385 No Hit GATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATA 3715 0.6033047785311193 No Hit AAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGC 3374 0.547927408550201 No Hit TGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGAT 3355 0.5448418659412935 No Hit GTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTG 3158 0.5128496609963055 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2787 0.4526003816329016 No Hit TACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACC 2677 0.43473671389712154 No Hit ATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATAC 2222 0.3608460882627583 No Hit GCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAG 2119 0.3441191993828915 No Hit ACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTG 2013 0.3269051195647761 No Hit GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 1947 0.316186918923308 No Hit CAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGT 1908 0.3098534367260769 No Hit GCTTTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC 1585 0.25739921237464986 No Hit GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA 1530 0.2484673785067598 No Hit CTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCT 1459 0.2369371929682108 No Hit GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT 1175 0.19081645081401485 No Hit AACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACG 1172 0.1903292598757663 No Hit CACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACT 1054 0.17116641630465673 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1038 0.1685680646339978 No Hit AGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAG 955 0.15508911534245462 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 916 0.1487556331452235 No Hit AGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTA 915 0.14859323616580733 No Hit ATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCA 850 0.13803743250375544 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 821 0.13332792010068611 No Hit TTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGA 775 0.12585765904754173 No Hit GCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCT 681 0.11059234298242053 No Hit CTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCA 658 0.10685721245584831 No Hit CAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGA 658 0.10685721245584831 No Hit CTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTG 653 0.10604522755876741 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 1.6239697941618285E-4 0.0 12-13 0.0 0.0 0.0 1.6239697941618285E-4 0.0 14-15 0.0 0.0 0.0 1.6239697941618285E-4 0.0 16-17 0.0 0.0 0.0 1.6239697941618285E-4 0.0 18-19 0.0 0.0 0.0 1.6239697941618285E-4 0.0 20-21 0.0 0.0 0.0 5.6838942795664E-4 0.0 22-23 0.0 0.0 0.0 0.0016239697941618286 0.0 24-25 0.0 0.0 0.0 0.0034915350574479314 0.0 26-27 0.0 0.0 0.0 0.0065770776663554055 0.0 28-29 0.0 0.0 0.0 0.021923592221184687 0.0 30-31 0.0 0.0 0.0 0.05350980471763225 0.0 32-33 0.0 0.0 0.0 0.12496447566075271 0.0 34-35 0.0 0.0 0.0 0.2200479071089278 0.0 36-37 0.0 0.0 0.0 0.3390036945312817 0.0 38-39 0.0 0.0 0.0 0.5052982014534529 0.0 40-41 0.0 0.0 0.0 0.6758962283301531 0.0 42-43 0.0 0.0 0.0 0.8567252649100727 0.0 44-45 0.0 0.0 0.0 1.0743372173277579 0.0 46-47 0.0 0.0 0.0 1.3379887134099306 0.0 48-49 0.0 0.0 0.0 1.5751695018472656 0.0 50-51 0.0 0.0 0.0 1.8297267670821324 0.0 52-53 0.0 0.0 0.0 2.066501563070927 0.0 54-55 0.0 0.0 0.0 2.3436320084446427 0.0 56-57 0.0 0.0 0.0 2.7415046080142913 0.0 58-59 0.0 0.0 0.0 3.086029799845723 0.0 60-61 0.0 0.0 0.0 3.4834964069668306 0.0 62-63 0.0 0.0 0.0 3.8778774714790307 0.0 64-65 0.0 0.0 0.0 4.272826925419187 0.0 66-67 0.0 0.0 0.0 4.772522431082782 0.0 68-69 0.0 0.0 0.0 5.344159798627746 0.0 70-71 0.0 0.0 0.0 5.93617798708944 0.0 72-73 0.0 0.0 0.0 6.4841053956396415 0.0 74-75 0.0 0.0 0.0 7.155698104015265 0.0 76-77 0.0 0.0 0.0 7.752912995818278 0.0 78-79 0.0 0.0 0.0 8.614591368600543 0.0 80-81 0.0 0.0 0.0 9.514839023994153 0.0 82-83 0.0 0.0 0.0 10.27225853599123 0.0 84-85 0.0 0.0 0.0 11.128090617514514 0.0 86-87 0.0 0.0 0.0 11.966140229791726 0.0 88-89 0.0 0.0 0.0 12.887743087978563 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CATTCCG 110 7.2475814E-8 34.41121 9 GTAAGCG 90 0.0 34.36212 94-95 CTTTGCG 670 0.0 33.900364 2 CGCAGAA 140 4.7475623E-10 33.796722 9 GCTTTGC 700 0.0 33.174706 1 TTTGCGT 685 0.0 33.158024 3 CCCTATA 100 1.3371318E-6 33.12348 2 TTGCGTT 700 0.0 33.12348 4 CTTGCGT 65 0.005951831 29.119543 2 ACTCAAT 130 1.0318185E-5 25.477531 8 GACATAT 200 2.1000233E-8 23.69622 1 CAGTAGG 100 0.0016906551 23.65771 9 CATGGGG 565 0.0 23.450254 4 GAGTACT 2795 0.0 23.276815 12-13 AGGGTCT 105 0.0022442413 22.53298 5 TAGGCAT 210 3.5814082E-8 22.53298 5 GTAAGGT 105 0.0022442413 22.53298 4 CTACACG 150 3.120766E-5 22.082321 4 CATATAG 215 4.5947672E-8 22.008959 3 CCTTTAG 195 4.3078398E-7 21.873432 1 >>END_MODULE