##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938886_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 615775 Sequences flagged as poor quality 0 Sequence length 101 %GC 40 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.963939750720638 33.0 31.0 34.0 30.0 34.0 2 32.19503714830904 34.0 31.0 34.0 30.0 34.0 3 32.2787479192887 34.0 31.0 34.0 30.0 34.0 4 35.85264422881735 37.0 35.0 37.0 35.0 37.0 5 35.78214607608299 37.0 35.0 37.0 35.0 37.0 6 35.86980796557184 37.0 35.0 37.0 35.0 37.0 7 35.82919897689903 37.0 35.0 37.0 35.0 37.0 8 35.825860095002234 37.0 35.0 37.0 33.0 37.0 9 37.42069911899639 39.0 37.0 39.0 34.0 39.0 10-11 37.33183143193537 39.0 37.0 39.0 34.0 39.0 12-13 37.303960050343065 39.0 37.0 39.0 34.0 39.0 14-15 38.34746863708335 40.0 38.0 41.0 33.0 41.0 16-17 38.381733587755264 40.0 38.0 41.0 33.5 41.0 18-19 38.52354350209086 40.0 38.0 41.0 34.0 41.0 20-21 38.519634606796316 40.0 38.0 41.0 34.0 41.0 22-23 38.447603426576265 40.0 38.0 41.0 34.0 41.0 24-25 38.315262068125534 40.0 37.5 41.0 33.5 41.0 26-27 38.08348260322358 40.0 37.0 41.0 33.0 41.0 28-29 37.935569810401525 40.0 37.0 41.0 33.0 41.0 30-31 37.82794040030855 40.0 37.0 41.0 33.0 41.0 32-33 37.58515123218708 40.0 36.0 41.0 33.0 41.0 34-35 37.43746335918152 40.0 36.0 41.0 32.5 41.0 36-37 37.26170110835938 39.5 35.5 41.0 31.5 41.0 38-39 37.09860013803743 39.0 35.0 41.0 31.0 41.0 40-41 37.09212455848321 40.0 35.0 41.0 31.0 41.0 42-43 36.887024481344646 39.5 35.0 41.0 31.0 41.0 44-45 36.63400998741423 39.0 35.0 41.0 30.5 41.0 46-47 36.30720636596159 39.0 35.0 41.0 30.0 41.0 48-49 35.94568064633998 39.0 35.0 41.0 28.5 41.0 50-51 35.69264504080224 39.0 35.0 40.0 28.0 41.0 52-53 35.613841906540536 39.0 35.0 40.0 28.0 41.0 54-55 35.44575453696561 38.5 35.0 40.0 27.5 41.0 56-57 35.107686249035766 38.0 34.5 40.0 26.5 41.0 58-59 34.8575250700337 38.0 34.0 40.0 26.0 41.0 60-61 34.29350249685356 38.0 34.0 40.0 25.0 41.0 62-63 34.080048719093824 37.0 33.0 40.0 24.5 41.0 64-65 33.87116966424425 37.0 33.0 39.5 24.5 41.0 66-67 33.32496041573627 36.5 33.0 39.0 22.5 40.0 68-69 32.83959238358167 36.0 32.0 39.0 22.0 40.0 70-71 32.24568470626446 35.0 31.5 37.5 21.5 40.0 72-73 31.665398075595796 35.0 31.0 37.0 19.5 39.0 74-75 31.219733668953758 35.0 31.0 37.0 18.0 39.0 76-77 29.232435548698795 32.5 28.5 34.5 13.0 36.5 78-79 30.233841094555643 34.0 30.0 35.5 12.5 37.0 80-81 30.179788883926758 34.0 30.0 35.0 9.5 37.0 82-83 29.8103471235435 34.0 29.5 35.0 7.0 36.5 84-85 29.36109617961106 34.0 29.0 35.0 2.0 36.0 86-87 28.89444845925866 34.0 29.0 35.0 2.0 36.0 88-89 28.24947180382445 33.5 28.5 35.0 2.0 35.0 90-91 27.71730745808128 33.0 26.5 35.0 2.0 35.0 92-93 27.370719824611264 33.0 26.0 35.0 2.0 35.0 94-95 26.89880963014088 33.0 25.5 35.0 2.0 35.0 96-97 26.144775283179733 32.0 25.0 34.0 2.0 35.0 98-99 25.259518492956033 32.0 21.5 34.0 2.0 35.0 100-101 20.821945515813404 27.5 2.0 32.0 2.0 34.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.057931098065797215 1101 2 0.10727647869860846 1101 3 0.13762945567123808 1101 4 0.1462453925790541 1101 5 0.11276401886537712 1101 6 0.17458560020763514 1101 7 0.007143845773022406 1101 8 0.24164618792130454 1101 9 0.13833132401450854 1101 10-11 0.16027298166847714 1101 12-13 0.2170504580588002 1101 14-15 0.34028518328887003 1101 16-17 0.34776405117075626 1101 18-19 0.31492987573290776 1101 20-21 0.32926560981312747 1101 22-23 0.37196419336711983 1101 24-25 0.2980800216448358 1101 26-27 0.338758640008507 1101 28-29 0.5108656918391645 1101 30-31 0.36583082048166915 1101 32-33 0.21782291838524515 1101 34-35 0.544109340538121 1101 36-37 0.4471686134060917 1101 38-39 0.4400346458230473 1101 40-41 0.5370624881377637 1101 42-43 0.4863104709232928 1101 44-45 0.7008324454815948 1101 46-47 0.5542507306296258 1101 48-49 0.22105545065991095 1101 50-51 0.5941116474863932 1101 52-53 0.5611644630621768 1101 54-55 0.5786392771187465 1101 56-57 0.31990804440616216 1101 58-59 0.39373542423933117 1101 60-61 0.6955719146080739 1101 62-63 0.38941238024305136 1101 64-65 0.2651325893447094 1101 66-67 0.3593043572361623 1101 68-69 0.34963687055127224 1101 70-71 0.4233848284490236 1101 72-73 0.6019130266033521 1101 74-75 0.463752264664798 1101 76-77 0.37328400436688014 1101 78-79 0.5611554695115402 1101 80-81 0.7119550113138509 1101 82-83 0.5346860260304034 1101 84-85 0.4626721398422333 1101 86-87 0.8006082704692083 1101 88-89 0.10085814421927708 1101 90-91 0.5804421307766283 1101 92-93 0.6117669042849592 1101 94-95 0.7915462005402141 1101 96-97 0.5362637555601957 1101 98-99 0.330532662748535 1101 100-101 1.1500032272089271 1102 1 0.022347235256592768 1102 2 0.10814008982685408 1102 3 0.020360317431773467 1102 4 0.09049004127438565 1102 5 -0.07908410894906837 1102 6 0.028009201466041134 1102 7 -0.15452724176013533 1102 8 0.09039086745988811 1102 9 0.17092339112943478 1102 10-11 0.3062217201793729 1102 12-13 0.15690765379009264 1102 14-15 0.02999124707272216 1102 16-17 0.031443343009627256 1102 18-19 -0.010060871290718865 1102 20-21 0.16091233117516168 1102 22-23 0.35633900059678325 1102 24-25 0.17473013576487517 1102 26-27 0.10579799600134265 1102 28-29 -0.01565179628617841 1102 30-31 0.0631983487032457 1102 32-33 0.2690628172193712 1102 34-35 0.42026287825941466 1102 36-37 0.443253062237126 1102 38-39 0.2778638975656804 1102 40-41 0.19333446395665277 1102 42-43 0.1158197547429225 1102 44-45 0.08407376310542958 1102 46-47 0.24816844082640444 1102 48-49 0.17739990812706452 1102 50-51 0.257258584751213 1102 52-53 0.3617211838073544 1102 54-55 0.2792052508402918 1102 56-57 0.3476561783892649 1102 58-59 0.13658133133944972 1102 60-61 0.02513671628594949 1102 62-63 0.017310104010867633 1102 64-65 0.4001196351777878 1102 66-67 0.2322949192002639 1102 68-69 0.37958875507413126 1102 70-71 0.30725843279176246 1102 72-73 0.5721508280961665 1102 74-75 0.45756202347054753 1102 76-77 0.29523514865162426 1102 78-79 0.06036742435701825 1102 80-81 0.26529842011035143 1102 82-83 0.3272157897710777 1102 84-85 0.5491350354069695 1102 86-87 0.6814869947921984 1102 88-89 0.7803674280389075 1102 90-91 0.5906666696699716 1102 92-93 0.5761383187566125 1102 94-95 0.48514930954027236 1102 96-97 0.40600035416608193 1102 98-99 0.6668151252739811 1102 100-101 0.9693527429698321 1103 1 -0.044894144053749585 1103 2 -0.05910128948349325 1103 3 0.07036031743177062 1103 4 0.04738659299852799 1103 5 0.07608830484403484 1103 6 0.05387127043155715 1103 7 -0.06659620727737803 1103 8 -0.009609132540113308 1103 9 -0.08424902266366985 1103 10-11 -0.261019659130973 1103 12-13 -0.30343717379610524 1103 14-15 -0.08983633913417322 1103 16-17 -0.21683251905933787 1103 18-19 -0.2445436299114121 1103 20-21 -0.11494973779036144 1103 22-23 -0.15228168905839112 1103 24-25 -0.3597526228558152 1103 26-27 -0.21316752124003102 1103 28-29 -0.18547938249307094 1103 30-31 -0.1868016512967472 1103 32-33 -0.16110959657373058 1103 34-35 -0.03835781139576255 1103 36-37 -0.1334710756938975 1103 38-39 -0.00575679208948543 1103 40-41 -0.07045863949162623 1103 42-43 0.010647340949823558 1103 44-45 -0.11334002999802095 1103 46-47 0.2378236132401952 1103 48-49 -0.11053112635569562 1103 50-51 -0.29446555317981904 1103 52-53 -0.36155467826161924 1103 54-55 -0.27510509398729965 1103 56-57 -0.5135507181624632 1103 58-59 -0.4616945307295097 1103 60-61 -0.3662425940588818 1103 62-63 -0.29475886150638075 1103 64-65 -0.2550527786153154 1103 66-67 0.1417776778209543 1103 68-69 -0.07041124492587159 1103 70-71 -0.318603636173755 1103 72-73 -0.14509055121417092 1103 74-75 0.017906851056753936 1103 76-77 -0.11597174790009745 1103 78-79 -0.03963257564298317 1103 80-81 0.20150531666207527 1103 82-83 0.4306640656331453 1103 84-85 0.30258331126903926 1103 86-87 0.16855596030944042 1103 88-89 0.1769191521768363 1103 90-91 0.6372183938079026 1103 92-93 0.8390693532393705 1103 94-95 0.5351493095402731 1103 96-97 0.609448630028151 1103 98-99 0.8383668494119121 1103 100-101 1.0538355015905196 1104 1 -0.013576241361260344 1104 2 -0.042958162416887546 1104 3 -0.06396892205807347 1104 4 -0.01369040275017852 1104 5 -0.0275489176406154 1104 6 -0.05350945701293597 1104 7 -0.20887301407945102 1104 8 -0.17283539612064658 1104 9 -0.27331373688190297 1104 10-11 -0.11168097231000473 1104 12-13 -0.15718067875595665 1104 14-15 -0.16882783653615974 1104 16-17 -0.11286463998517604 1104 18-19 -0.06764235452170198 1104 20-21 0.08337336093425307 1104 22-23 -0.2143506545756324 1104 24-25 -0.22096542398949026 1104 26-27 -0.16800337386166575 1104 28-29 -0.3020830669522141 1104 30-31 -0.20418474057402847 1104 32-33 0.025918036869818195 1104 34-35 -0.003491254947952882 1104 36-37 -0.12163238887293204 1104 38-39 0.2784661649251561 1104 40-41 0.2853455645707257 1104 42-43 0.06408971222993642 1104 44-45 0.08235552975634874 1104 46-47 0.1327574819222903 1104 48-49 0.1437556001440683 1104 50-51 -0.0046627662171374595 1104 52-53 0.10214395187536951 1104 54-55 0.338073932937597 1104 56-57 0.1950994944024842 1104 58-59 0.15244103847218327 1104 60-61 0.3649040757568969 1104 62-63 0.14989158724184648 1104 64-65 -0.10817039788786786 1104 66-67 0.2631581690821747 1104 68-69 -0.03153547733021611 1104 70-71 0.0991160142749905 1104 72-73 -0.20039877039225473 1104 74-75 -0.11083665390309605 1104 76-77 0.26302565408384027 1104 78-79 0.10845433980340147 1104 80-81 0.17448594963326158 1104 82-83 0.1676916990766948 1104 84-85 0.19152993384816241 1104 86-87 0.19262894094240934 1104 88-89 0.4303497142930368 1104 90-91 0.4828194802509813 1104 92-93 0.4903860750154685 1104 94-95 0.6617553558321951 1104 96-97 0.1107240197305579 1104 98-99 0.013555796034491863 1104 100-101 0.8092145757520726 1105 1 -0.1136568215385374 1105 2 -0.0778349898601931 1105 3 0.04778715023385871 1105 4 -0.06649344177400707 1105 5 0.1059781918333087 1105 6 -0.018252751591042227 1105 7 -0.012119244078306224 1105 8 0.05683537629790436 1105 9 -0.24610065987027951 1105 10-11 -0.027529366462182736 1105 12-13 -0.05692167104328405 1105 14-15 -0.04402353125239955 1105 16-17 0.03621732156656776 1105 18-19 0.021908123203630225 1105 20-21 0.1282478860867755 1105 22-23 0.016321614331928913 1105 24-25 0.14645572255131611 1105 26-27 -0.06626082752806184 1105 28-29 0.08155915706067418 1105 30-31 0.16623370077511623 1105 32-33 0.09828623072269949 1105 34-35 0.14963842158019247 1105 36-37 0.16199400689085763 1105 38-39 0.05318989003163921 1105 40-41 0.23914002756140462 1105 42-43 0.385922623476624 1105 44-45 0.23573270254327383 1105 46-47 0.1439667601541359 1105 48-49 0.30776791739974385 1105 50-51 0.20791781396013675 1105 52-53 0.2644957419064511 1105 54-55 0.10659586225727224 1105 56-57 0.20253157682449086 1105 58-59 0.3981069760801077 1105 60-61 0.27318800576725266 1105 62-63 0.07000497506273717 1105 64-65 0.07401850507057617 1105 66-67 0.13517814145468066 1105 68-69 -0.04917102469985224 1105 70-71 -0.16598845216912395 1105 72-73 -0.21000651760072842 1105 74-75 -0.042510419299667035 1105 76-77 -0.0697460162281196 1105 78-79 0.05059489465547884 1105 80-81 0.15163571364845652 1105 82-83 0.4180010694001659 1105 84-85 0.002235585972023557 1105 86-87 0.07837919995012399 1105 88-89 0.376940885007901 1105 90-91 0.3561114682790709 1105 92-93 0.17563846364215507 1105 94-95 -0.020704066292818624 1105 96-97 0.37128577868071844 1105 98-99 0.6878017958042619 1105 100-101 1.3799366316977384 1106 1 -0.05221457021940523 1106 2 -0.11914825935403428 1106 3 -0.054167119846262324 1106 4 -0.04095113182676613 1106 5 0.013990699161823272 1106 6 -0.08301726204638982 1106 7 -0.11807976782268526 1106 8 0.08626668370459356 1106 9 -0.1270660692898602 1106 10-11 0.04782728782743817 1106 12-13 0.13016060372708438 1106 14-15 -0.024588315305557273 1106 16-17 -0.01634277223840286 1106 18-19 -0.009565396436784113 1106 20-21 -0.13942848678794917 1106 22-23 0.02152974409546715 1106 24-25 -0.15745281760893448 1106 26-27 -0.050265126922241166 1106 28-29 0.022940826007328496 1106 30-31 -0.248403782125024 1106 32-33 -0.06686913374866066 1106 34-35 -0.03504987234202872 1106 36-37 -0.04414240116073387 1106 38-39 -0.10430473572564125 1106 40-41 0.06715448915999644 1106 42-43 0.25675514253075704 1106 44-45 0.1405206642395811 1106 46-47 0.26444751059384686 1106 48-49 0.16234090938722545 1106 50-51 0.07737257260778563 1106 52-53 0.37000678353240346 1106 54-55 0.24488001312692376 1106 56-57 0.17238696083857263 1106 58-59 0.13357697803437674 1106 60-61 0.17685226216749328 1106 62-63 0.3476285826452994 1106 64-65 -0.08057402961771487 1106 66-67 -0.13334833890490216 1106 68-69 -0.02162558792048941 1106 70-71 0.03308326954282137 1106 72-73 0.28987622619446185 1106 74-75 0.40574050897862435 1106 76-77 0.2550243570374633 1106 78-79 0.4004092389978666 1106 80-81 0.41448503942527637 1106 82-83 0.6525623357671826 1106 84-85 0.4052353905450161 1106 86-87 0.38379252807206754 1106 88-89 0.7608376995476718 1106 90-91 1.0031996058596846 1106 92-93 0.8121246860380857 1106 94-95 0.9191337292905324 1106 96-97 0.6652568554835305 1106 98-99 0.3843915945144438 1106 100-101 1.0584494321667606 1107 1 -0.07469500171691124 1107 2 -0.14428310232494113 1107 3 -0.15712602457610103 1107 4 -0.2514561491738263 1107 5 -0.14906883549550543 1107 6 -0.12414083084318861 1107 7 -0.06899296460569815 1107 8 -0.18646985039242736 1107 9 -0.21963937689030644 1107 10-11 -0.03444531853296695 1107 12-13 -0.0958577224655599 1107 14-15 0.053299418370379215 1107 16-17 -0.059881629086945054 1107 18-19 -0.12628450989731022 1107 20-21 -0.2920175284265554 1107 22-23 -0.03504912135587546 1107 24-25 -0.08361886264903262 1107 26-27 -0.09279655255060959 1107 28-29 -0.002206751346975011 1107 30-31 -0.05927271985105875 1107 32-33 0.04569149606563627 1107 34-35 -0.2783346074951467 1107 36-37 -0.2195457981282587 1107 38-39 -0.060099446145763125 1107 40-41 -0.20822372202702155 1107 42-43 -0.22722641808207555 1107 44-45 0.09726620862031865 1107 46-47 0.009326873535094649 1107 48-49 -0.22572527544809873 1107 50-51 -0.22474076906421203 1107 52-53 -0.190515495978822 1107 54-55 -0.22413581712657304 1107 56-57 0.04329002671211413 1107 58-59 -0.30790994516880943 1107 60-61 -0.531520453425621 1107 62-63 -0.4401033953723257 1107 64-65 -0.27679248372017184 1107 66-67 -0.2938256235947776 1107 68-69 -0.2667808625020953 1107 70-71 -0.3639026433992747 1107 72-73 -0.20986908613751254 1107 74-75 -0.36467553747640835 1107 76-77 -0.5779217567117101 1107 78-79 -0.230910552497825 1107 80-81 -0.018450037858283252 1107 82-83 -0.26589940731522077 1107 84-85 -0.2272234207993371 1107 86-87 -0.2569507071404793 1107 88-89 -0.05390532059529818 1107 90-91 -0.11392417800669818 1107 92-93 0.17726473595687153 1107 94-95 0.07670191483898847 1107 96-97 0.1551103817757884 1107 98-99 0.10462426584849638 1107 100-101 0.82853296972187 1108 1 -0.03498035095030261 1108 2 0.061684151129533404 1108 3 -0.057333648085467814 1108 4 0.02250632479929493 1108 5 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-1.5045038583315886 2316 72-73 -1.372051335527896 2316 74-75 -1.44181013918762 2316 76-77 -1.6310905540432472 2316 78-79 -1.6132706515630062 2316 80-81 -1.3770757709453854 2316 82-83 -1.9251361855017919 2316 84-85 -1.9112097921792355 2316 86-87 -1.997811276240352 2316 88-89 -2.2486893670929433 2316 90-91 -2.372459960292357 2316 92-93 -2.84845117221219 2316 94-95 -2.993673253967888 2316 96-97 -3.722551756333292 2316 98-99 -4.156876075344197 2316 100-101 -4.509559166621592 >>END_MODULE >>Per sequence quality scores pass #Quality Count 3 4.0 4 16.0 5 52.0 6 137.0 7 208.0 8 289.0 9 492.0 10 744.0 11 1143.0 12 1586.0 13 2222.0 14 2558.0 15 3501.0 16 4337.0 17 6079.0 18 6015.0 19 4127.0 20 3750.0 21 4032.0 22 4422.0 23 4988.0 24 5785.0 25 6643.0 26 7939.0 27 9516.0 28 11477.0 29 13644.0 30 16900.0 31 20200.0 32 24559.0 33 31446.0 34 43431.0 35 62938.0 36 85894.0 37 128349.0 38 90804.0 39 5548.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.838042969622556 22.79163955996654 16.33951121524432 18.030806255166578 2 14.12074215419593 26.519589135642075 36.152977954610044 23.206690755551946 3 23.4350209086111 26.41435589298039 33.02716089480736 17.12346230360115 4 11.219195322966993 19.47789371117697 42.268685802444075 27.03422516341196 5 9.895529683828293 41.51870900477936 36.22260151062161 12.363159800770738 6 24.526003816329016 40.87548211603264 17.158215257196215 17.440298810442126 7 22.110511144492712 36.53493565019691 23.769721083187854 17.584832122122528 8 22.591530997523446 30.60241159514433 21.49242824083472 25.313629166497503 9 20.756769924079414 22.232633673013684 27.137022451382403 29.873573951524502 10-11 23.291380780317485 26.56757744305956 28.68969997158053 21.451341805042425 12-13 27.801226097194593 26.25009134830092 27.36494661199302 18.58373594251147 14-15 21.814547037195826 29.05712865884554 31.079400033778953 18.04892427017968 16-17 17.29563246689657 31.475032053910656 31.252542591709915 19.976792887482855 18-19 16.917624132191143 33.57663107466201 31.296577483658805 18.209167309488045 20-21 20.014209735698916 28.58747107303804 29.475457756485728 21.922861434777314 22-23 18.122041330031262 28.89399537168609 31.490966668019976 21.492996630262677 24-25 21.95964435061508 29.539442166375707 29.940643904023386 18.56026957898583 26-27 20.37554301489992 26.409727579067027 35.37745117940806 17.837278226624985 28-29 17.001420973569893 32.968454386748405 31.845479274085502 18.1846453655962 30-31 18.84162234582437 28.71868783240632 30.871259794567823 21.568430027201494 32-33 19.32369777922131 29.405951849295604 32.51788396735821 18.752466404124885 34-35 21.779363533977143 28.051779956297874 31.271462316050343 18.89739419367464 36-37 20.115293947595582 27.152626027133625 34.33571992941717 18.396360095853627 38-39 18.780377083913635 30.024029618392767 32.82069064647713 18.374902651216463 40-41 18.483298796292086 30.803522128607714 31.879340136805663 18.83383893829454 42-43 17.666236042960602 31.00636841306097 29.32726075415717 22.000134789821256 44-45 19.665185331944294 27.630695377439917 30.407446894028407 22.29667239658738 46-47 22.05124457490388 30.632092209379273 30.22691010072713 17.08975311498971 48-49 20.181413813011094 29.212398369857794 31.177890607681867 19.428297209449237 50-51 15.794401174460676 30.46338579863976 34.463648883028135 19.27856414387143 52-53 19.002476553936095 32.644634809792535 28.66298566846657 19.689902967804798 54-55 17.520604116763426 28.844220697495025 30.79257845804068 22.842596727700865 56-57 20.73768827899801 28.455361130282974 29.660021923592222 21.14692866712679 58-59 23.337314775643968 28.023453428898776 29.329859112157315 19.309372683299948 60-61 19.597639036288978 30.124536044971666 32.51984724704453 17.757977671694825 62-63 18.059437294466324 29.942836263245503 32.10701960943527 19.89070683285291 64-65 17.69806592846233 33.14002632461449 29.870479973561707 19.291427773361473 66-67 19.534479608615335 29.251294127200016 27.999918799861963 23.21430746432269 68-69 17.777590115693677 29.480775102149526 30.43820359748215 22.30343118467465 70-71 21.541066136169867 29.67536843814705 28.547359019122243 20.23620640656084 72-73 20.147375258820187 26.678250984531687 33.03511834679875 20.139255409849376 74-75 17.52401445333117 33.138159230238315 29.143761926028176 20.19406439040234 76-77 18.86052166673848 28.724759536234806 29.351741721062847 23.062977075963868 78-79 19.142072300817915 29.07728397327268 30.799830132621604 20.980813593287802 80-81 21.00190816450814 28.351183467987497 29.519142543948686 21.127765823555684 82-83 19.161219601315416 27.98522187487313 32.22037270106776 20.633185822743698 84-85 18.41191993828915 29.364297024075352 30.796232390077545 21.427550647557954 86-87 18.28194280352341 30.23512986743707 29.839790995673386 21.64313633336613 88-89 18.259856985940782 29.615603846652526 28.229253053665744 23.895286113740948 90-91 19.308518394515332 26.799514812633447 29.770106234527322 24.121860558323903 92-93 20.77812512687264 29.477325321749014 29.9498193333604 19.794730218017946 94-95 19.52774958385774 27.984247492996627 30.345174779749094 22.142828143396535 96-97 16.29248836622026 28.59015072961318 32.51782519976972 22.599535704396843 98-99 18.7917664731436 29.703057123137512 28.216393975072062 23.288782428646826 100-101 17.85393841414349 27.236756312578713 30.28097136208141 24.628333911196385 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 8701.0 1 5216.5 2 1528.5 3 1194.5 4 877.5 5 849.0 6 1516.5 7 2758.5 8 3662.0 9 3579.5 10 2547.0 11 1546.5 12 1286.5 13 1204.5 14 1123.5 15 1022.5 16 979.0 17 1030.0 18 1033.0 19 1070.5 20 1193.0 21 1348.5 22 1574.0 23 1835.0 24 2084.0 25 2736.5 26 4273.5 27 5764.5 28 6132.0 29 6678.5 30 8112.5 31 9785.0 32 11980.0 33 14578.5 34 17524.0 35 19381.5 36 21220.0 37 23586.5 38 22730.0 39 20233.5 40 18329.0 41 16721.5 42 15357.5 43 14811.0 44 14864.0 45 14867.5 46 17485.0 47 35570.5 48 69396.5 49 64659.0 50 29689.5 51 15113.5 52 11233.0 53 8155.0 54 5328.0 55 3816.0 56 3153.0 57 2762.5 58 2593.0 59 2343.5 60 2164.5 61 1977.5 62 1506.5 63 938.0 64 547.5 65 388.5 66 258.5 67 176.5 68 112.5 69 72.0 70 49.5 71 38.5 72 33.0 73 36.0 74 34.5 75 31.5 76 21.0 77 6.0 78 2.0 79 1.5 80 1.0 81 0.5 82 1.0 83 0.5 84 0.0 85 0.5 86 0.5 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.39917177540497745 2 0.0 3 0.0 4 0.0 5 3.247939588323657E-4 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0011367788559132799 16-17 0.0025171531809508344 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0025171531809508344 36-37 0.007226665584020138 38-39 0.012423368925337989 40-41 0.0017863667735780115 42-43 2.4359546912427427E-4 44-45 2.4359546912427427E-4 46-47 4.0599244854045715E-4 48-49 8.119848970809142E-5 50-51 4.8719093824854855E-4 52-53 0.0 54-55 0.0 56-57 0.0 58-59 2.4359546912427427E-4 60-61 0.0015427713044537373 62-63 0.0 64-65 2.4359546912427427E-4 66-67 0.0020299622427022855 68-69 0.0017863667735780115 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.12431488774308798 78-79 8.119848970809142E-5 80-81 0.0 82-83 0.0 84-85 0.0 86-87 0.13958020380820918 88-89 0.42523649060127483 90-91 0.32341358450732816 92-93 0.0 94-95 0.0 96-97 5.683894279566399E-4 98-99 0.0 100-101 8.119848970809142E-5 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 615775.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 28.9553571582384 #Duplication Level Percentage of deduplicated Percentage of total 1 77.0717836465971 22.31641022309696 2 11.367129062212504 6.582785637203091 3 3.737215773736561 3.2463725251783324 4 1.856244007955381 2.1499283289275195 5 1.097216666767895 1.5885150233118126 6 0.6617752741182473 1.1497163652350981 7 0.5044026471631073 1.0223611160118076 8 0.36683951390936165 0.8497575316000103 9 0.2971250955381896 0.7743026935785587 >10 2.4847486204451528 14.763367226981137 >50 0.3306129338413215 6.666921564996995 >100 0.19566887921221066 10.26070976098585 >500 0.009558537202895347 2.0101704655193626 >1k 0.01293213856862312 9.899442387292853 >5k 0.005622668942879616 10.694588783469783 >10k+ 0.0011245337885759234 6.024650366610838 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 23800 3.8650481101051524 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 13205 2.1444521131906944 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 9236 1.4998985018878648 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 7090 1.1513945840607365 No Hit ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC 6915 1.1229751126629046 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 6456 1.0484348991108765 No Hit TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC 6390 1.0377166984694086 No Hit GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT 6275 1.0190410458365475 No Hit GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG 6151 0.9989038203889408 No Hit GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA 6078 0.9870488408915594 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5603 0.9099102756688725 No Hit TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT 5495 0.8923714018919248 No Hit ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC 4928 0.8002923145629491 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4737 0.7692744914944583 No Hit GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA 4298 0.697982217530754 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4171 0.6773578011448987 No Hit GATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATA 3836 0.6229548130404774 No Hit AAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGC 3715 0.6033047785311193 No Hit CCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCAC 3647 0.5922617839308189 No Hit TGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGAT 3405 0.5529617149121026 No Hit GTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTG 3088 0.5014818724371727 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2871 0.46624172790386104 No Hit TACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACC 2605 0.42304413137915636 No Hit GCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAG 2335 0.37919694693678696 No Hit ATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATAC 2208 0.35857253055093175 No Hit ACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTG 2071 0.3363241443709147 No Hit CAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGT 2070 0.3361617473914985 No Hit GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 1831 0.29734886931103083 No Hit GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA 1578 0.25626243351873657 No Hit GCTTTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC 1560 0.25333928788924526 No Hit CTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCT 1296 0.21046648532337298 No Hit AACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACG 1264 0.20526978198205514 No Hit AGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAG 1140 0.18513255653444846 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1102 0.1789614713166335 No Hit GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT 1049 0.17035443140757583 No Hit CACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACT 999 0.16223458243676667 No Hit AGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTA 996 0.16174739149851813 No Hit ATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCA 987 0.16028581868377248 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 952 0.1546019244042061 No Hit TTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGA 843 0.13690065364784215 No Hit CTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTG 779 0.12650724696520646 No Hit CAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGA 694 0.11270350371483091 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 693 0.11254110673541472 No Hit GCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCT 649 0.10539563964110268 No Hit TACTAACAGTGTTGCATCTATAAAGTTATAGATTAACCCAATTTTAAGTT 639 0.10377166984694086 No Hit CTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCA 635 0.10312208192927612 No Hit GTTATAGATTAACCCAATTTTAAGTTTAGGAAGTTGGTGTAAATTATGGA 628 0.10198530307336283 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 1.6239697941618285E-4 0.0 12-13 0.0 0.0 0.0 1.6239697941618285E-4 0.0 14-15 0.0 0.0 0.0 1.6239697941618285E-4 0.0 16-17 0.0 0.0 0.0 1.6239697941618285E-4 0.0 18-19 0.0 0.0 0.0 1.6239697941618285E-4 0.0 20-21 0.0 0.0 0.0 5.6838942795664E-4 0.0 22-23 0.0 0.0 0.0 0.0016239697941618286 0.0 24-25 0.0 0.0 0.0 0.0035727335471560226 0.0 26-27 0.0 0.0 0.0 0.006983070114895863 0.0 28-29 0.0 0.0 0.0 0.022654378628557507 0.0 30-31 0.0 0.0 0.0 0.05464658357354553 0.0 32-33 0.0 0.0 0.0 0.12642604847549835 0.0 34-35 0.0 0.0 0.0 0.22272745726929477 0.0 36-37 0.0 0.0 0.0 0.34395680240347526 0.0 38-39 0.0 0.0 0.0 0.5132556534448459 0.0 40-41 0.0 0.0 0.0 0.686452031992205 0.0 42-43 0.0 0.0 0.0 0.8702042142016159 0.0 44-45 0.0 0.0 0.0 1.0909829077179163 0.0 46-47 0.0 0.0 0.0 1.3573951524501644 0.0 48-49 0.0 0.0 0.0 1.5959563152125371 0.0 50-51 0.0 0.0 0.0 1.850026389509155 0.0 52-53 0.0 0.0 0.0 2.0877755673744467 0.0 54-55 0.0 0.0 0.0 2.3667735780114487 0.0 56-57 0.0 0.0 0.0 2.7661889488855507 0.0 58-59 0.0 0.0 0.0 3.1105517437375667 0.0 60-61 0.0 0.0 0.0 3.5093987251837118 0.0 62-63 0.0 0.0 0.0 3.9036985912062034 0.0 64-65 0.0 0.0 0.0 4.296374487434534 0.0 66-67 0.0 0.0 0.0 4.793552839917178 0.0 68-69 0.0 0.0 0.0 5.364703016523892 0.0 70-71 0.0 0.0 0.0 5.95745199139296 0.0 72-73 0.0 0.0 0.0 6.505622995412285 0.0 74-75 0.0 0.0 0.0 7.181194429783606 0.0 76-77 0.0 0.0 0.0 7.779383703463116 0.0 78-79 0.0 0.0 0.0 8.640656083796841 0.0 80-81 0.0 0.0 0.0 9.54220291502578 0.0 82-83 0.0 0.0 0.0 10.298160854208113 0.0 84-85 0.0 0.0 0.0 11.152774958385773 0.0 86-87 0.0 0.0 0.0 11.984003897527506 0.0 88-89 0.0 0.0 0.0 12.90024765539361 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTAAGCG 135 0.0 42.172817 94-95 CGTCACT 50 0.0015930373 38.10716 1 AACTGCC 70 2.0663919E-4 33.88887 7 CTCCCGC 100 1.3098233E-6 33.21109 7 GCGTACA 30 0.0039187884 31.71976 74-75 CGTAACT 60 0.003972242 31.629612 4 CTTAGGT 80 4.5221453E-4 29.65276 3 CTTTGCG 495 0.0 28.754192 2 TTTGCGT 505 0.0 28.184803 3 CTCCGAC 95 4.0199666E-10 27.467817 94-95 CGTACTC 35 0.00843986 27.111097 94-95 TTGCGTT 555 0.0 26.500484 4 ATAGTAC 90 9.0041483E-4 26.35801 3 GCTTTGC 545 0.0 26.22052 1 GGTACTC 130 1.010256E-5 25.546993 3 TATAGGG 95 0.0012343038 24.970743 2 CCGGGTC 115 1.2910302E-4 24.75361 9 CATTCCG 115 1.2910302E-4 24.75361 9 CGTATAG 100 0.0016259003 23.816975 1 CGCTGTA 70 1.7946852E-5 23.724136 10-11 >>END_MODULE