FastQCFastQC Report
Thu 26 May 2016
SRR938885_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938885_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences335460
Sequences flagged as poor quality0
Sequence length101
%GC39

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT30040.895486794252668No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT27170.8099326298217373No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA20370.6072258987658737No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT20310.6054373099624396No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT18030.5374709354319442No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT17000.506766827639659No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT11820.3523519942765158No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT7770.2316222500447147No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT6620.1973409646455613No Hit
CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC5560.16574256245155905No Hit
GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT5400.16097299230906814No Hit
TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC5350.15948250163953975No Hit
GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG5190.15471293149704882No Hit
ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC5130.15292434269361474No Hit
GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA5100.1520300482918977No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4990.1487509688189352No Hit
TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT4230.1260955106421034No Hit
ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC4040.12043164609789542No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGGATCG205.4751267E-447.34313218-19
GGTACTC751.3914541E-744.200113
TCCAGAC459.758765E-442.0953453
CTCCCGC802.3291614E-741.394367
ACACGGA851.67459E-533.3937726
TACTCCC1051.9414256E-631.5715075
CGCAGAA1551.4097168E-930.5211879
GTCGGGA650.005924731529.142932
GTGGTAC1153.8499074E-628.916781
TGGTACT1153.9451934E-628.8261592
GTCTAGC700.00837791927.1463641
CTTTGCG1057.034121E-527.061292
CTACACG1057.034121E-527.061294
TACACGG1057.034121E-527.061295
ACGGAGG1109.721248E-525.8042778
CGGAGGT1109.721248E-525.8042779
GTGTAGC1309.983323E-625.5802271
GCAGATC1151.3147984E-424.6823528
CATGGGG5350.023.8999274
CACGGAG1201.7548702E-423.653927
Produced by FastQC (version 0.11.5)