##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938885_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 335460 Sequences flagged as poor quality 0 Sequence length 101 %GC 39 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.973949204077982 33.0 31.0 34.0 30.0 34.0 2 32.08141954331366 34.0 31.0 34.0 30.0 34.0 3 32.07729684612174 34.0 31.0 34.0 30.0 34.0 4 35.63573898527395 37.0 35.0 37.0 35.0 37.0 5 35.675400941990105 37.0 35.0 37.0 35.0 37.0 6 35.775371132176716 37.0 35.0 37.0 35.0 37.0 7 35.73230489477136 37.0 35.0 37.0 35.0 37.0 8 35.72604483395934 37.0 35.0 37.0 35.0 37.0 9 37.369820544923385 39.0 37.0 39.0 35.0 39.0 10-11 37.27716568294282 39.0 37.0 39.0 34.0 39.0 12-13 37.21091933464497 39.0 37.0 39.0 34.0 39.0 14-15 38.41614350444166 40.0 38.0 41.0 34.0 41.0 16-17 38.32670810230728 40.0 38.0 41.0 34.0 41.0 18-19 38.30698891074942 40.0 38.0 41.0 34.0 41.0 20-21 38.41066744172181 40.0 38.0 41.0 34.0 41.0 22-23 38.33868270434627 40.0 38.0 41.0 34.0 41.0 24-25 38.26084779109283 40.0 38.0 41.0 34.0 41.0 26-27 38.12574226435342 40.0 38.0 41.0 34.0 41.0 28-29 38.00772074166816 40.0 38.0 41.0 34.0 41.0 30-31 37.77017826268408 40.0 37.5 41.0 33.0 41.0 32-33 37.506413581350984 40.0 36.5 41.0 33.0 41.0 34-35 37.17537858463006 40.0 36.0 41.0 32.5 41.0 36-37 36.90168425445657 40.0 35.5 41.0 31.0 41.0 38-39 36.66246646395994 39.5 35.0 41.0 31.0 41.0 40-41 36.52685715137423 39.0 35.0 41.0 30.5 41.0 42-43 36.26465748524414 39.0 35.0 41.0 30.0 41.0 44-45 36.03992279258332 39.5 35.0 41.0 30.0 41.0 46-47 35.68271925117749 40.0 35.0 41.0 27.5 41.0 48-49 35.34357449472366 40.0 35.0 41.0 24.5 41.0 50-51 34.235069754963334 38.0 34.0 40.0 23.0 40.5 52-53 34.356587968759314 38.5 34.5 39.5 23.0 40.5 54-55 34.876702140344605 39.0 35.0 41.0 23.0 41.0 56-57 34.80806802599416 39.0 35.0 41.0 22.5 41.0 58-59 34.63448250163954 39.0 35.0 41.0 22.0 41.0 60-61 34.365758957848925 39.0 35.0 41.0 20.5 41.0 62-63 34.04213169975556 38.0 34.5 40.0 18.5 41.0 64-65 33.707394324211535 38.0 34.0 40.0 15.5 41.0 66-67 33.299478328265664 37.0 34.0 40.0 10.5 41.0 68-69 32.85353395337745 37.0 33.0 39.0 7.5 41.0 70-71 32.39926965957193 36.0 33.0 39.0 2.0 41.0 72-73 31.940714243128838 36.0 32.5 39.0 2.0 40.0 74-75 31.455300184820842 35.0 32.0 37.5 2.0 40.0 76-77 31.023405174983605 35.0 31.5 37.0 2.0 39.0 78-79 30.60122965480236 35.0 31.0 37.0 2.0 39.0 80-81 30.101500924104215 35.0 31.0 36.0 2.0 38.5 82-83 29.608151493471652 35.0 30.0 36.0 2.0 37.0 84-85 29.150573838907768 34.0 30.0 35.0 2.0 37.0 86-87 28.716760567578845 34.0 29.0 35.0 2.0 36.5 88-89 28.2399481309247 34.0 29.0 35.0 2.0 36.0 90-91 27.764848268049843 34.0 28.0 35.0 2.0 36.0 92-93 27.27981130388124 34.0 26.5 35.0 2.0 35.0 94-95 26.77981577535325 34.0 25.5 35.0 2.0 35.0 96-97 26.19830084063674 34.0 24.5 35.0 2.0 35.0 98-99 25.484336433553928 33.0 20.5 35.0 2.0 35.0 100-101 22.559108388481487 29.5 2.0 33.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.42020634171960936 1101 2 0.5197078644188693 1101 3 0.5257777241658985 1101 4 0.30874241960412974 1101 5 0.3167852892231551 1101 6 0.2351217649300068 1101 7 0.31914176172002584 1101 8 0.3243147238599491 1101 9 0.0714627587246639 1101 10-11 0.22041686532755733 1101 12-13 0.45945433631150223 1101 14-15 0.4004812239481268 1101 16-17 0.24197218275216414 1101 18-19 0.739241108108871 1101 20-21 0.5053714010528481 1101 22-23 0.45903971990576053 1101 24-25 0.5406472030695681 1101 26-27 0.6305857789170872 1101 28-29 0.6311977900499031 1101 30-31 0.673154582128781 1101 32-33 0.4862426124497716 1101 34-35 0.5452943343457335 1101 36-37 0.6488113895886869 1101 38-39 0.6529854094715049 1101 40-41 0.797019355858751 1101 42-43 0.6091361415755401 1101 44-45 0.807127030201805 1101 46-47 0.978553498371511 1101 48-49 1.2379659260430103 1101 50-51 1.0769884578700086 1101 52-53 0.943095854607968 1101 54-55 1.0357376264634865 1101 56-57 0.8345949752240998 1101 58-59 0.8989305316546492 1101 60-61 0.9466250181193772 1101 62-63 0.9057019394054677 1101 64-65 1.1475379998652784 1101 66-67 1.2432133338941682 1101 68-69 1.4201459340471132 1101 70-71 1.2861908573013139 1101 72-73 0.9922983829810548 1101 74-75 0.8393089363073223 1101 76-77 0.521748958959698 1101 78-79 0.4982335793615107 1101 80-81 0.321977063868907 1101 82-83 0.32833158290385356 1101 84-85 0.42045399878398015 1101 86-87 0.6055651311381887 1101 88-89 0.3958269094137279 1101 90-91 0.5272172239150343 1101 92-93 0.7791190554600682 1101 94-95 1.1792014044890564 1101 96-97 1.2509563459991746 1101 98-99 1.3035612723208203 1101 100-101 0.9751686148621097 1102 1 0.25453972985046747 1102 2 0.4211545190300754 1102 3 0.39598979058049366 1102 4 0.21503865501428976 1102 5 0.3132097508374869 1102 6 0.3861163399752172 1102 7 0.30775757628520495 1102 8 0.4268545890580384 1102 9 0.4037412397373217 1102 10-11 0.41409186121774155 1102 12-13 0.28055412260116697 1102 14-15 0.3264429204794439 1102 16-17 0.6459463731187611 1102 18-19 0.3337462864748062 1102 20-21 0.4614169377945885 1102 22-23 0.36866490484739245 1102 24-25 0.43449070134344225 1102 26-27 0.34828263901901124 1102 28-29 0.6207177637470949 1102 30-31 0.6685515901840162 1102 32-33 0.5936320584468149 1102 34-35 0.7614294650378284 1102 36-37 1.1014457805142186 1102 38-39 1.1204562297904346 1102 40-41 1.1558883349808937 1102 42-43 1.1965724394548758 1102 44-45 1.6206442092253113 1102 46-47 1.394898229589657 1102 48-49 1.390830466565781 1102 50-51 1.5450141031108515 1102 52-53 1.5351145953608807 1102 54-55 1.4305880292252766 1102 56-57 1.4993985261035974 1102 58-59 1.4440151938279229 1102 60-61 1.4439125407233604 1102 62-63 1.285078891567224 1102 64-65 1.3395896191320915 1102 66-67 1.211238979135004 1102 68-69 1.3828699189229994 1102 70-71 1.3240011482761602 1102 72-73 1.134128072279097 1102 74-75 1.2807235343674996 1102 76-77 1.139084155408824 1102 78-79 1.1090136221035785 1102 80-81 0.7123066709706691 1102 82-83 0.9617978002308298 1102 84-85 0.8911510232784714 1102 86-87 0.8780252659400958 1102 88-89 1.098687340122261 1102 90-91 1.1388603276467464 1102 92-93 0.9805821493282245 1102 94-95 0.8329495550726485 1102 96-97 1.183185509240996 1102 98-99 0.8455224756744322 1102 100-101 0.5319251494667441 1103 1 0.233859249321565 1103 2 0.23672333302668846 1103 3 0.47240275518583275 1103 4 0.38929085206338954 1103 5 0.4088317777664585 1103 6 0.45099983590362314 1103 7 0.4960587937326011 1103 8 0.2560088259362132 1103 9 0.4536283638202079 1103 10-11 0.609830795346582 1103 12-13 0.43031627691866703 1103 14-15 0.4652198292918257 1103 16-17 0.5584272245256798 1103 18-19 0.6692497936908097 1103 20-21 0.9909057711413567 1103 22-23 0.7220370728712595 1103 24-25 0.7415736651425249 1103 26-27 0.8434652561277787 1103 28-29 0.8382558593691272 1103 30-31 0.9117185659156846 1103 32-33 1.0147499331545475 1103 34-35 0.7434600221611092 1103 36-37 0.7623443534401204 1103 38-39 0.8899777165275111 1103 40-41 0.9197962604827836 1103 42-43 1.234446340930326 1103 44-45 1.1229823532227314 1103 46-47 1.0256831203418955 1103 48-49 0.8839974422974493 1103 50-51 1.0194154576218537 1103 52-53 1.0308615920552313 1103 54-55 1.2585228030703206 1103 56-57 1.0171059356013004 1103 58-59 0.9727683180720561 1103 60-61 1.2319980845433989 1103 62-63 1.0686393204957128 1103 64-65 0.8251374704583867 1103 66-67 1.1315002062574777 1103 68-69 1.1436032092559785 1103 70-71 1.299601809406532 1103 72-73 1.4815137854130143 1103 74-75 1.4157110373909596 1103 76-77 1.6111070530948695 1103 78-79 1.398328707163646 1103 80-81 1.2507449520317024 1103 82-83 1.2407726450264462 1103 84-85 1.3179227720489273 1103 86-87 1.4023742137752997 1103 88-89 1.6261706102988285 1103 90-91 1.8817854264131775 1103 92-93 1.834045021214063 1103 94-95 1.5786864735601114 1103 96-97 1.4195496953250064 1103 98-99 1.5525893143424945 1103 100-101 1.8539944069044587 1104 1 0.3295001728884408 1104 2 0.573725721561722 1104 3 0.5304043475425217 1104 4 0.46681871830542576 1104 5 0.49742652298938594 1104 6 0.5587824792157221 1104 7 0.5788414370447015 1104 8 0.35588939918462614 1104 9 0.5152127587246653 1104 10-11 0.47839170298989586 1104 12-13 0.5835208947530575 1104 14-15 0.6165932369351381 1104 16-17 0.4598902022326854 1104 18-19 0.49563315356341775 1104 20-21 0.4441701023515492 1104 22-23 0.5501720251005651 1104 24-25 0.39434038488774803 1104 26-27 0.4530492529430674 1104 28-29 0.36098675108886624 1104 30-31 0.3757114003105997 1104 32-33 0.4843757293328892 1104 34-35 0.552944496683395 1104 36-37 0.5950573311471317 1104 38-39 0.84777031206891 1104 40-41 0.7817190311834281 1104 42-43 0.909744907809305 1104 44-45 1.3582431016303715 1104 46-47 1.3241703814883934 1104 48-49 1.0183027766923587 1104 50-51 0.8796264448829945 1104 52-53 0.9012736143482201 1104 54-55 1.0635785355543916 1104 56-57 1.2630852349643575 1104 58-59 1.1313371874988079 1104 60-61 1.0769821609765202 1104 62-63 1.091091549795081 1104 64-65 1.3617019609042416 1104 66-67 1.491163820907154 1104 68-69 1.5993058366445112 1104 70-71 1.1812598508078054 1104 72-73 1.072696110253787 1104 74-75 1.092373059683954 1104 76-77 0.7221142186999572 1104 78-79 0.6834202676731991 1104 80-81 0.31030904438839 1104 82-83 0.423684667319435 1104 84-85 0.42236146631644544 1104 86-87 0.22086545581351302 1104 88-89 0.3088139224007378 1104 90-91 0.6918073213176328 1104 92-93 0.49784876325227856 1104 94-95 0.7069408525410026 1104 96-97 1.1271332940511236 1104 98-99 1.1750913047883564 1104 100-101 1.6561142317452244 1105 1 0.48455560102300055 1105 2 0.36614735764734974 1105 3 0.39150908760368 1105 4 0.6143244522503792 1105 5 0.4962701774236393 1105 6 0.6703554914481415 1105 7 0.4034878437725311 1105 8 0.3260919985730055 1105 9 0.46442911958093447 1105 10-11 0.6402657473323998 1105 12-13 0.5033699008692665 1105 14-15 0.6944315396874359 1105 16-17 0.7266667695109774 1105 18-19 0.8462399960710627 1105 20-21 0.8538413561741862 1105 22-23 0.5658400296877204 1105 24-25 0.6827100332057867 1105 26-27 0.7903915694568227 1105 28-29 0.7210593810582893 1105 30-31 0.6771162168243521 1105 32-33 0.5301134969169823 1105 34-35 0.5359959110564816 1105 36-37 0.7050487302296986 1105 38-39 0.713839692802857 1105 40-41 0.9708149791956586 1105 42-43 0.7289631799805605 1105 44-45 0.5677757851166163 1105 46-47 0.5145755802651593 1105 48-49 0.3435846956526021 1105 50-51 0.47913905956189495 1105 52-53 0.36185752107605396 1105 54-55 0.1688346517623387 1105 56-57 0.25756153465854226 1105 58-59 0.32732820431837695 1105 60-61 0.537112130395478 1105 62-63 0.467161886186517 1105 64-65 0.4732558599868142 1105 66-67 0.6216636297756608 1105 68-69 0.44697881065062717 1105 70-71 0.26478909392707806 1105 72-73 0.13585551392350226 1105 74-75 -0.00558661156987128 1105 76-77 -0.052608640627255454 1105 78-79 0.21330367745913392 1105 80-81 0.5345301835321195 1105 82-83 0.3638175037108766 1105 84-85 0.7335522155519207 1105 86-87 0.5422435139174873 1105 88-89 0.8327435860093004 1105 90-91 1.035552543030171 1105 92-93 0.8011314467385162 1105 94-95 0.6620392852627148 1105 96-97 0.8102468261612152 1105 98-99 0.4606465417914123 1105 100-101 0.5343300192070011 1106 1 0.17112825930819398 1106 2 0.3768507215617234 1106 3 0.3312531129746219 1106 4 0.3636165578115964 1106 5 0.4147490538535834 1106 6 0.26422229403053876 1106 7 0.29384915309407944 1106 8 0.3206270535056106 1106 9 0.39862325255182185 1106 10-11 0.5225853758294008 1106 12-13 0.5734514503086174 1106 14-15 0.5076040394042778 1106 16-17 0.4466378874178716 1106 18-19 0.4698808387486082 1106 20-21 0.5785836825984632 1106 22-23 0.7287406979400686 1106 24-25 0.4122801997025576 1106 26-27 0.42405619738750744 1106 28-29 0.4255700844221977 1106 30-31 0.539175906483429 1106 32-33 0.7667059762464703 1106 34-35 0.37738508310314955 1106 36-37 0.5625920533693503 1106 38-39 0.7099037997232358 1106 40-41 1.0387020558747864 1106 42-43 1.0219748460809157 1106 44-45 0.9294815275562982 1106 46-47 1.100867912352598 1106 48-49 0.8879979927417381 1106 50-51 0.5238779880928703 1106 52-53 0.8571571020025388 1106 54-55 0.9216803874062407 1106 56-57 0.9693352349643547 1106 58-59 1.0856388850296668 1106 60-61 1.1681858646802326 1106 62-63 1.1210298214000147 1106 64-65 1.103947331274611 1106 66-67 0.9925719999194982 1106 68-69 0.9431137070148807 1106 70-71 1.0590183384621241 1106 72-73 0.7982748139574838 1106 74-75 0.8808954362271599 1106 76-77 0.9219213174653902 1106 78-79 0.7729650207596208 1106 80-81 0.9012619764871559 1106 82-83 1.006882969788574 1106 84-85 1.1364432564399003 1106 86-87 1.2419688508752422 1106 88-89 0.9489373791908626 1106 90-91 0.9780148830460256 1106 92-93 0.7801211397954866 1106 94-95 0.906574340195327 1106 96-97 0.8301811335572928 1106 98-99 0.9810905331834192 1106 100-101 1.1491504971773274 1107 1 -0.1628477010118523 1107 2 -0.12314927843827661 1107 3 -0.021666766826982098 1107 4 -0.2319990969438379 1107 5 -0.36220507525108303 1107 6 -0.38710278471389614 1107 7 -0.1690232990256817 1107 8 -0.14625502896787168 1107 9 -0.21152844362137557 1107 10-11 -0.32718472662887876 1107 12-13 -0.2643621697630678 1107 14-15 -0.34569598593876094 1107 16-17 -0.2975648930752328 1107 18-19 -0.34177062209640496 1107 20-21 -0.4300601029270439 1107 22-23 -0.2727889353099897 1107 24-25 -0.15583373534685308 1107 26-27 -0.356531942071598 1107 28-29 -0.45358436328063334 1107 30-31 -0.27186008062782463 1107 32-33 -0.3341909862096344 1107 34-35 -0.22895158102921442 1107 36-37 -0.35537796943938105 1107 38-39 -0.1225696805997103 1107 40-41 -0.3399726843590969 1107 42-43 -0.3288089778211898 1107 44-45 -0.45978750687402936 1107 46-47 -0.2101771258429821 1107 48-49 -0.09430261187069533 1107 50-51 -0.15374049060087458 1107 52-53 0.14858391493473277 1107 54-55 -0.16147644978285314 1107 56-57 -0.21468236034356636 1107 58-59 -0.07755669080031424 1107 60-61 -0.0010603610176147527 1107 62-63 -0.22291138275623723 1107 64-65 -0.20447289246818912 1107 66-67 0.022015177212146853 1107 68-69 0.05844714896122838 1107 70-71 -0.1546868134150685 1107 72-73 0.025656154242049922 1107 74-75 -0.13372206494947747 1107 76-77 -0.09607676370472973 1107 78-79 -0.2965925622388248 1107 80-81 -0.18394040575823567 1107 82-83 -0.47141705555446123 1107 84-85 -0.12227746330232137 1107 86-87 -0.2534105705794474 1107 88-89 -0.26310689871363024 1107 90-91 -0.12428047193749947 1107 92-93 -0.19270750507616796 1107 94-95 -0.09378770317746898 1107 96-97 0.3870581474235557 1107 98-99 0.39394669335140264 1107 100-101 0.2060546642965484 1108 1 0.24978281620053977 1108 2 0.13481250500121433 1108 3 0.16030084435780623 1108 4 0.26827174378313146 1108 5 0.004373179040342734 1108 6 0.13889792507559662 1108 7 0.25402057717209203 1108 8 0.020340036127301175 1108 9 -0.20879201834539884 1108 10-11 0.27225117751218875 1108 12-13 0.4016538565364982 1108 14-15 0.43974212228545895 1108 16-17 0.3721214920416074 1108 18-19 0.26638355165258787 1108 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-0.7501186912696625 2316 66-67 -0.6904897916248132 2316 68-69 -0.6999548730741552 2316 70-71 -0.6355839791154665 2316 72-73 -0.731515846267957 2316 74-75 -0.6309845170423891 2316 76-77 -0.4365414718371241 2316 78-79 -0.35245728987155545 2316 80-81 -0.42544463208219696 2316 82-83 -0.04121702705396402 2316 84-85 -0.40868872549941315 2316 86-87 -0.01701018357267614 2316 88-89 -0.14201727964529454 2316 90-91 -0.387208823183645 2316 92-93 -0.383293340328283 2316 94-95 -0.5533732459244689 2316 96-97 -1.0378842890690798 2316 98-99 -1.3206208115799285 2316 100-101 -1.1096746109657722 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 1633.0 3 317.0 4 280.0 5 298.0 6 289.0 7 357.0 8 474.0 9 490.0 10 667.0 11 956.0 12 1379.0 13 2231.0 14 2080.0 15 2484.0 16 3332.0 17 4946.0 18 4118.0 19 2210.0 20 1895.0 21 1957.0 22 2141.0 23 2264.0 24 2556.0 25 2877.0 26 3438.0 27 4059.0 28 4569.0 29 5675.0 30 6720.0 31 8340.0 32 10373.0 33 13304.0 34 18129.0 35 27733.0 36 38746.0 37 64403.0 38 74369.0 39 13369.0 40 2.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 40.55069360116288 22.893623894096464 16.66497179534728 19.890710709393375 2 14.804913488821722 23.547032900802307 40.879403506992205 20.768650103383766 3 17.44785605585892 28.815981857786532 33.350938441798704 20.385223644555843 4 11.438964635387292 20.256692598527643 41.201817915961676 27.102524850123395 5 10.952305630746917 39.186023260741756 36.62716260135912 13.234508507152215 6 25.07061578154594 40.60817562226895 19.385858947399818 14.93534964878529 7 23.42726071485031 35.90239188209712 24.785613510748405 15.884733892304158 8 22.93760234807742 35.23995311003929 22.685553895118016 19.136890646765277 9 24.93579399192839 18.069255985849324 24.63989452615817 32.35505549606412 10-11 22.056246485899603 28.460447448446192 29.656099136034648 19.82720692961956 12-13 24.323510111555212 27.675087991409654 29.46026964147229 18.541132255562847 14-15 21.7528768201965 28.91912690222092 29.296290109942554 20.031706167640024 16-17 18.20134164128843 31.497400532719283 30.623901974879274 19.677355851113013 18-19 18.063354706113092 29.797353832111074 33.80851600124687 18.330775460528965 20-21 19.367117305237034 28.585470187454508 33.67409643585858 18.37331607144988 22-23 19.366233952221403 28.44398829254321 33.54974028182629 18.640037473409095 24-25 19.315601493102903 29.16682829018234 33.51708683807711 18.000483378637647 26-27 18.803258145363408 28.751491824800095 34.36373672275928 18.081513307077216 28-29 18.06735726561008 29.020073515371397 34.51239378460951 18.400175434409014 30-31 18.747380244545315 28.861389018581807 33.80241561963463 18.58881511723825 32-33 18.534649271307053 29.181874182448425 33.82429931075834 18.459177235486177 34-35 19.348283457219885 28.72478344151912 33.55419083463799 18.372742266623003 36-37 19.227081797863224 28.43301155871937 33.4479318341153 18.891974809302102 38-39 18.482615265499646 29.408857625935802 33.456085518187436 18.65244159037711 40-41 18.520581418330128 29.076905570428398 33.840008145127 18.562504866114473 42-43 18.482138845203323 29.21965101475324 32.737362390474054 19.560847749569383 44-45 18.619306402713036 28.986566645496055 32.71878632458149 19.675340627209433 46-47 19.47948853517781 29.43588791149867 32.135052594843714 18.949570958479807 48-49 19.06934169880121 29.514191632734388 31.890200237900483 19.526266430563922 50-51 18.37050508440045 29.716178400436235 32.310211345673316 19.60310516949 52-53 18.828842999348357 30.169202076264522 31.54064519994907 19.461309724438053 54-55 19.04502982315811 29.664980033013684 31.345172722670096 19.944817421158113 56-57 19.32122116095951 29.802712958886303 30.90281209659787 19.97325378355632 58-59 19.494055640403676 29.22783218039709 31.502859880813343 19.77525229838589 60-61 19.345312930301613 29.97443634230157 31.32835231150544 19.351898415891373 62-63 18.903945036988 29.642671523510106 32.01218005269236 19.441203386809537 64-65 19.022650221198834 29.78761016870437 31.49397976626182 19.695759843834974 66-67 19.14087725212571 29.63613889201034 30.570925572161666 20.65205828370229 68-69 18.87762518075629 29.27255426434999 31.380790757265842 20.469029797627876 70-71 19.691650221245236 29.29682524698101 31.11069154682862 19.900832984945136 72-73 19.374388524563145 28.711365614741634 31.53512734420767 20.37911851648755 74-75 18.834953492832142 30.177068849786643 30.5828079301913 20.40516972718991 76-77 18.89523649751804 29.355709402682372 31.18579786644458 20.563256233355002 78-79 19.103741797128883 29.016673805024006 31.137065446257157 20.742518951589954 80-81 19.207924350844824 29.42038058210551 30.624638692613903 20.74705637443576 82-83 19.33366184926511 29.025730585981556 30.94650033176115 20.694107232992188 84-85 18.980831044182363 29.068123335560074 30.691448410974743 21.25959720928282 86-87 18.812019106190938 29.402055308498483 30.87845640670005 20.907469178610533 88-89 19.359221323139515 28.724344436888288 30.63613513602579 21.280299103946408 90-91 19.342732711002846 28.32128073636948 30.860790632383058 21.475195920244616 92-93 19.841996365287144 28.730112831914756 30.755873447268943 20.672017355529153 94-95 19.309622521924535 27.939790501391215 31.188502299210523 21.56208467747373 96-97 19.196471377888578 28.028364416530827 30.966235976603702 21.808928228976892 98-99 19.32471813312357 28.25785668801738 30.481776978686742 21.935648200172302 100-101 19.49451237505486 27.402174678284524 31.307098786236526 21.79621416042409 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 2878.0 1 1683.0 2 476.5 3 421.0 4 339.5 5 346.0 6 553.0 7 1015.0 8 1439.5 9 1502.0 10 1181.0 11 884.0 12 889.5 13 915.0 14 899.0 15 885.0 16 898.0 17 919.5 18 955.5 19 1014.5 20 1079.5 21 1185.0 22 1313.0 23 1542.0 24 1739.0 25 2060.5 26 2750.0 27 3369.0 28 3555.5 29 3905.0 30 5012.0 31 6095.0 32 7439.5 33 9514.5 34 11452.0 35 13095.0 36 15585.5 37 17729.5 38 17375.5 39 16277.5 40 15024.0 41 13595.0 42 12278.0 43 11776.0 44 11804.0 45 11471.0 46 11093.5 47 11171.5 48 13008.5 49 12151.0 50 8595.0 51 6430.5 52 5122.5 53 4378.0 54 3838.5 55 3474.5 56 3063.5 57 2716.5 58 2602.0 59 2332.0 60 2025.0 61 1748.0 62 1410.0 63 972.5 64 607.0 65 484.5 66 362.5 67 258.5 68 213.5 69 164.5 70 109.5 71 82.5 72 73.5 73 70.5 74 59.0 75 51.5 76 43.5 77 24.5 78 13.0 79 11.0 80 5.0 81 3.5 82 3.5 83 1.5 84 1.0 85 1.5 86 1.5 87 0.5 88 0.0 89 0.5 90 0.5 91 0.0 92 0.5 93 0.5 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.3332737137065522 2 0.0897275383056102 3 0.09837238418887498 4 0.1046324450008943 5 0.1159601740893102 6 0.058129136111607936 7 0.059917724915042034 8 0.061706313718476125 9 0.06289870625409885 10-11 0.060662970249806235 12-13 0.059619626781136345 14-15 0.05783103797770226 16-17 0.05932152864723067 18-19 0.06573063852620283 20-21 0.06915876706611816 22-23 0.08615036069874203 24-25 0.09509330471591247 26-27 0.08942944017170452 28-29 0.08704465510045907 30-31 0.07944315268586419 32-33 0.07050020866869373 34-35 0.3223931318189948 36-37 0.5025934537649794 38-39 0.47382698384308114 40-41 0.4525129672688249 42-43 0.48739044893578964 44-45 0.49603529481905445 46-47 0.4872413998688368 48-49 0.5088535145769987 50-51 0.5049782388362248 52-53 0.5030406009658379 54-55 0.494544804149526 56-57 0.47129314970488284 58-59 0.4560901448756931 60-61 0.414952602396709 62-63 0.48753949800274254 64-65 0.5098968580456686 66-67 0.5209264890001788 68-69 0.5343409050259346 70-71 0.5307637274190664 72-73 0.5182436057950277 74-75 0.5210755380671317 76-77 0.49216001907828055 78-79 0.4718893459726942 80-81 0.4778513086508078 82-83 0.48753949800274254 84-85 0.4885828414714124 86-87 0.48932808680617657 88-89 0.4957371966851487 90-91 0.5110892505812914 92-93 0.5167531151254994 94-95 0.5242055684731414 96-97 0.5163059679246408 98-99 0.5209264890001788 100-101 0.49588624575210155 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 335460.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 41.00917144775072 #Duplication Level Percentage of deduplicated Percentage of total 1 78.17227669832481 32.0578029758261 2 10.666336515771647 8.748352457893668 3 3.566027978396983 4.387195582606733 4 1.619668953625938 2.656851272313804 5 1.0144719823564288 2.080132772669716 6 0.6766306155384817 1.6648836551648813 7 0.5249779705005182 1.5070238118983579 8 0.4130303562612331 1.3550426154433974 9 0.30484186761711995 1.1251181172206697 >10 2.5895780857538084 21.777687380542815 >50 0.3171644822596518 8.84005926240933 >100 0.12410784088421158 8.119187118927448 >500 0.005806214778208729 1.3726929503461778 >1k 0.0050804379309326375 4.307970026736899 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3004 0.895486794252668 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2717 0.8099326298217373 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 2037 0.6072258987658737 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2031 0.6054373099624396 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 1803 0.5374709354319442 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1700 0.506766827639659 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 1182 0.3523519942765158 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 777 0.2316222500447147 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 662 0.1973409646455613 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 556 0.16574256245155905 No Hit GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT 540 0.16097299230906814 No Hit TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC 535 0.15948250163953975 No Hit GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG 519 0.15471293149704882 No Hit ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC 513 0.15292434269361474 No Hit GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA 510 0.1520300482918977 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 499 0.1487509688189352 No Hit TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT 423 0.1260955106421034 No Hit ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC 404 0.12043164609789542 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.0 0.0 20-21 0.0 0.0 0.0 1.4904906695284087E-4 0.0 22-23 0.0 0.0 0.0 0.0014904906695284088 0.0 24-25 0.0 0.0 0.0 0.004322422941632385 0.0 26-27 0.0 0.0 0.0 0.008346747749359089 0.0 28-29 0.0 0.0 0.0 0.021761163775114768 0.0 30-31 0.0 0.0 0.0 0.05276336970130567 0.0 32-33 0.0 0.0 0.0 0.1071662791390926 0.0 34-35 0.0 0.0 0.0 0.1822870088833244 0.0 36-37 0.0 0.0 0.0 0.29630954510224766 0.0 38-39 0.0 0.0 0.0 0.44953198592976806 0.0 40-41 0.0 0.0 0.0 0.6011148870208072 0.0 42-43 0.0 0.0 0.0 0.7574673582543373 0.0 44-45 0.0 0.0 0.0 0.956298813569427 0.0 46-47 0.0 0.0 0.0 1.1853872294759435 0.0 48-49 0.0 0.0 0.0 1.4314672390150838 0.0 50-51 0.0 0.0 0.0 1.6857449472366302 0.0 52-53 0.0 0.0 0.0 1.9392774101234125 0.0 54-55 0.0 0.0 0.0 2.2191915578608477 0.0 56-57 0.0 0.0 0.0 2.5783998092171947 0.0 58-59 0.0 0.0 0.0 2.940439992845645 0.0 60-61 0.0 0.0 0.0 3.32215465331187 0.0 62-63 0.0 0.0 0.0 3.709980325523162 0.0 64-65 0.0 0.0 0.0 4.1221009956477666 0.0 66-67 0.0 0.0 0.0 4.594735586955226 0.0 68-69 0.0 0.0 0.0 5.12013354796399 0.0 70-71 0.0 0.0 0.0 5.706045430155607 0.0 72-73 0.0 0.0 0.0 6.2867405950038755 0.0 74-75 0.0 0.0 0.0 6.911554283670184 0.0 76-77 0.0 0.0 0.0 7.572139748405175 0.0 78-79 0.0 0.0 0.0 8.321558457044059 0.0 80-81 0.0 0.0 0.0 9.140135932749061 0.0 82-83 0.0 0.0 0.0 9.958117212186252 0.0 84-85 0.0 0.0 0.0 10.805610206880104 0.0 86-87 0.0 0.0 0.0 11.675460561616884 0.0 88-89 0.0 0.0 0.0 12.59971382579145 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGGATCG 20 5.4751267E-4 47.343132 18-19 GGTACTC 75 1.3914541E-7 44.20011 3 TCCAGAC 45 9.758765E-4 42.095345 3 CTCCCGC 80 2.3291614E-7 41.39436 7 ACACGGA 85 1.67459E-5 33.393772 6 TACTCCC 105 1.9414256E-6 31.571507 5 CGCAGAA 155 1.4097168E-9 30.521187 9 GTCGGGA 65 0.0059247315 29.14293 2 GTGGTAC 115 3.8499074E-6 28.91678 1 TGGTACT 115 3.9451934E-6 28.826159 2 GTCTAGC 70 0.008377919 27.146364 1 CTTTGCG 105 7.034121E-5 27.06129 2 CTACACG 105 7.034121E-5 27.06129 4 TACACGG 105 7.034121E-5 27.06129 5 ACGGAGG 110 9.721248E-5 25.804277 8 CGGAGGT 110 9.721248E-5 25.804277 9 GTGTAGC 130 9.983323E-6 25.580227 1 GCAGATC 115 1.3147984E-4 24.682352 8 CATGGGG 535 0.0 23.899927 4 CACGGAG 120 1.7548702E-4 23.65392 7 >>END_MODULE