FastQCFastQC Report
Thu 26 May 2016
SRR938885_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938885_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences335460
Sequences flagged as poor quality0
Sequence length101
%GC39

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT28370.8457044058904191No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT27040.8060573540809635No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA20070.5982829547487033No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT18020.5371728372980386No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT18000.5365766410302272No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT16200.4829189769272045No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT11120.3314851249031181No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT7730.230429857509092No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5970.177964585941692No Hit
CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC5580.16633875871937043No Hit
TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC5560.16574256245155905No Hit
ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC5490.16365587551421928No Hit
CTTATACACATCTCCGAGCCCACGAGACGCTACGCTATCTCGTATGCCGT5250.15650152030048292No Hit
GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA5010.14934716508674656No Hit
GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT4980.1484528706850295No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4800.14308710427472723No Hit
ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC4780.14249090800691588No Hit
GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG4640.13831753413223632No Hit
TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT4430.132057473320217No Hit
GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA3680.10970011327729087No Hit
ATCTAGGAGGCTGCTGACCTCCAACAGGAATTTCACCACTTAACCCTCTA3360.10016097299230906No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCAGGA407.5723237E-659.324039
ACGCAGG652.6018479E-643.8019878
GTAAGCG308.336417E-539.54345794-95
GCGCCTC250.001577511838.18368582-83
GTCCTAC500.001590366838.1151161
CAGTTGC550.002589800634.5106546
CTTTGCG702.062418E-433.8943942
CGGGTCG300.003966428331.63948414-15
AACGGAC300.003966428331.63948414-15
CGCAGAA1051.9091203E-631.6394849
ACTCCGG600.003966519631.6347688
GCGCAAT404.5143135E-429.66201642-43
ACGGAGG804.5134936E-429.6575978
GACATAT1452.320121E-829.572071
CATATAG1303.0528645E-729.2013243
GTAATAC650.005867073329.2013243
CTACACG650.005867073329.2013244
CGGAGGT700.00841843727.1195589
AACTGGG700.00842456227.1155157
TACTCCG700.00842456227.1155157
Produced by FastQC (version 0.11.5)