FastQCFastQC Report
Thu 26 May 2016
SRR938884_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938884_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences337428
Sequences flagged as poor quality0
Sequence length101
%GC39

[WARN]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT29120.8629989212513484No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT27420.8126178029090652No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA20220.5992389487535119No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT19350.5734556705430491No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT18360.5441160780966606No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT15770.4673589625045936No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT11210.3322190215394099No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT7800.2311604253351826No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT6480.19204096873999787No Hit
CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC6120.18137202603222022No Hit
GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA5490.1627013762936093No Hit
ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC5320.15766326445938098No Hit
GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT5070.1502542764678687No Hit
TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC5050.14966155742854773No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4940.1464016027122823No Hit
GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG4820.14284528847635644No Hit
TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT4730.14017805279941203No Hit
ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC3830.11350569602996786No Hit
GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA3800.1126166174709864No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3420.10135495572388775No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGTACG405.4209726E-447.4318161
GTACAGG500.001625623837.9454541
CCGGATT250.001633915537.91166318-19
CTTTGCG757.0332553E-637.9004172
GCTTTGC1054.6999048E-836.1385271
GTATGGT550.002595262634.495871
TTTGCGT1001.3224198E-633.162863
GGTACTC902.4515164E-531.5836818
TTGCGTT953.546557E-529.9213814
GAGTACT20200.024.50862312-13
GTACTTT21500.023.24533314-15
CAGAACC1100.002917274821.5343274
CGTAGAG1404.928396E-420.3037932
ACTTTTT24900.020.16938816-17
GGTTGCA1656.441695E-520.0957247
AGTACTT22300.020.07617212-13
GAGTACC958.43198E-619.94758612-13
GGATGAC1708.0941405E-519.5075688
GTGCATG1507.780363E-418.9727271
CATGGGT1751.01118625E-418.9502094
Produced by FastQC (version 0.11.5)