FastQCFastQC Report
Thu 26 May 2016
SRR938884_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938884_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences337428
Sequences flagged as poor quality0
Sequence length101
%GC39

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT27820.8244721836954847No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT26030.7714238296762569No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA19700.5838282537311663No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT18620.5518214256078333No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT18190.5390779662624323No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT16060.4759533885747478No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT12020.3562241426319096No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT8020.23768033476771344No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT6510.19293004729897933No Hit
CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC5690.1686285666868191No Hit
GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA5690.1686285666868191No Hit
ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC5390.1597377810970044No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5310.1573669049397205No Hit
TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC5240.15529238830209705No Hit
CTTATACACATCTCCGAGCCCACGAGACGCTACGCTATCTCGTATGCCGT5180.1535142311841341No Hit
GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG4870.1443270860746589No Hit
GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT4850.1437343670353379No Hit
TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT4370.12950911009163435No Hit
ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC4270.12654551489502947No Hit
GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA3940.11676565074623328No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTCACT250.00497306857.1302451
GATCTAC608.167662E-539.6737751
GCATGGC500.001622013737.9626543
CGGGTCG557.066228E-834.51150514-15
GTCACTA600.003903161531.739021
CGCAGAA1601.9153958E-929.6583259
ACAGTGC650.005866415329.2020448
GAGTGAT1153.8835497E-628.8846287
GTATAGT1503.249079E-828.565121
GGCATGG700.0084236227.1161822
TAGATAT1301.0070242E-525.5517887
GTAATGC1000.001660710123.726666
GTAGATA1401.7882072E-523.726666
CCCGGGT801.8736155E-623.7266612-13
GAGTACT21350.022.89317112-13
ATACTCA1252.2663566E-422.7775946
CTCCCCG1050.00220559622.596829
ATAGTAA1903.3038668E-722.4778883
GTACTTT22100.022.11625314-15
GGTCCTA1302.955131E-421.9015318
Produced by FastQC (version 0.11.5)