##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938884_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 337428 Sequences flagged as poor quality 0 Sequence length 101 %GC 39 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.104896451983834 33.0 31.0 34.0 30.0 34.0 2 32.33910938037152 34.0 31.0 34.0 30.0 34.0 3 32.39574961176903 34.0 31.0 34.0 30.0 34.0 4 36.009714665054474 37.0 35.0 37.0 35.0 37.0 5 35.93283011486895 37.0 35.0 37.0 35.0 37.0 6 35.98277262112214 37.0 35.0 37.0 35.0 37.0 7 35.936143414298755 37.0 35.0 37.0 35.0 37.0 8 35.91523228659151 37.0 35.0 37.0 35.0 37.0 9 37.56825456097301 39.0 37.0 39.0 35.0 39.0 10-11 37.492282798108036 39.0 37.0 39.0 34.5 39.0 12-13 37.44791629621727 39.0 37.0 39.0 34.0 39.0 14-15 38.63478579133919 40.0 38.0 41.0 34.0 41.0 16-17 38.59978425026969 40.0 38.0 41.0 34.0 41.0 18-19 38.72836130967199 40.0 38.0 41.0 34.0 41.0 20-21 38.744612183932574 40.0 38.0 41.0 34.0 41.0 22-23 38.67516773948813 40.0 38.0 41.0 34.0 41.0 24-25 38.59215891982882 40.0 38.0 41.0 34.0 41.0 26-27 38.40824709271311 40.0 38.0 41.0 34.0 41.0 28-29 38.21802428962623 40.0 38.0 41.0 33.5 41.0 30-31 37.99381794041988 40.0 37.0 41.0 33.0 41.0 32-33 37.73802707540572 40.0 36.5 41.0 33.0 41.0 34-35 37.64262450063421 40.0 36.0 41.0 33.0 41.0 36-37 37.49174638737746 40.0 36.0 41.0 33.0 41.0 38-39 37.329828882013345 40.0 36.0 41.0 33.0 41.0 40-41 37.15506567326956 40.0 36.0 41.0 32.0 41.0 42-43 36.927174093436236 40.0 36.0 41.0 31.0 41.0 44-45 36.558207973256515 40.0 35.0 41.0 30.5 41.0 46-47 36.12373009945826 40.0 35.0 41.0 29.5 41.0 48-49 35.710327832900646 39.0 35.0 41.0 27.5 41.0 50-51 35.41910126012068 39.0 35.0 41.0 27.0 41.0 52-53 35.24352306269782 39.0 35.0 41.0 26.0 41.0 54-55 35.02193504984767 39.0 35.0 40.0 25.5 41.0 56-57 34.747314982751874 38.5 35.0 40.0 24.5 41.0 58-59 34.479154071387086 38.0 34.0 40.0 23.0 41.0 60-61 34.080328247803976 38.0 34.0 40.0 22.0 41.0 62-63 33.80171177258556 38.0 34.0 40.0 22.0 41.0 64-65 33.5839719880982 37.0 33.5 40.0 21.5 41.0 66-67 33.12818882843155 37.0 33.0 39.5 19.0 41.0 68-69 32.695400203895346 36.0 32.5 39.0 17.5 40.0 70-71 32.23974151522695 36.0 32.0 39.0 13.5 40.0 72-73 31.718299903979513 35.0 31.5 38.0 8.0 40.0 74-75 31.228004196450797 35.0 31.0 37.0 7.0 39.0 76-77 29.237262467844992 33.0 28.5 35.5 2.0 37.5 78-79 30.1900316511967 34.0 30.0 36.0 2.0 38.5 80-81 30.136227580402338 34.0 30.0 36.0 2.0 37.5 82-83 29.777813340920137 34.0 30.0 35.5 2.0 37.0 84-85 29.350153217871664 34.0 29.5 35.0 2.0 37.0 86-87 28.803621809689773 34.0 29.0 35.0 2.0 36.0 88-89 28.230748485602852 34.0 29.0 35.0 2.0 36.0 90-91 27.773013798499235 33.5 27.5 35.0 2.0 35.0 92-93 27.41755870882084 33.0 27.0 35.0 2.0 35.0 94-95 27.028084509880628 33.0 26.0 35.0 2.0 35.0 96-97 26.441957988074492 33.0 25.0 35.0 2.0 35.0 98-99 25.62626545514895 33.0 22.5 34.5 2.0 35.0 100-101 21.49880418933817 28.0 2.0 32.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.21402686166437235 1101 2 0.3011016312458068 1101 3 0.3082860973964756 1101 4 0.3235326791067976 1101 5 0.310530827452304 1101 6 0.2339101563992969 1101 7 0.2554247218908827 1101 8 0.471589820188278 1101 9 0.11738940418512556 1101 10-11 0.38729719609094815 1101 12-13 0.5359677401207037 1101 14-15 0.655574611396375 1101 16-17 0.6306006901118693 1101 18-19 0.539911228770066 1101 20-21 0.4320849904363371 1101 22-23 0.5191331868198787 1101 24-25 0.3374276094258306 1101 26-27 0.4036198861293201 1101 28-29 0.49917860251153456 1101 30-31 0.28175661818711717 1101 32-33 0.3669979446036109 1101 34-35 0.3127737014315244 1101 36-37 0.09088986170798563 1101 38-39 0.2132899115227076 1101 40-41 0.23654435493788384 1101 42-43 0.04648430633316991 1101 44-45 0.1455569095978646 1101 46-47 0.24122361288129923 1101 48-49 0.3165949294754782 1101 50-51 0.2609043471726906 1101 52-53 -0.04495715204095063 1101 54-55 -0.16788532808415368 1101 56-57 0.022363351386346153 1101 58-59 0.18364916558459043 1101 60-61 -0.050241602695301424 1101 62-63 -0.022551322148380848 1101 64-65 -0.05502015317922826 1101 66-67 0.037926350376736195 1101 68-69 -0.06640412927720263 1101 70-71 0.2608626102744438 1101 72-73 0.353820622054112 1101 74-75 0.3273965436625019 1101 76-77 0.11893708917431312 1101 78-79 0.35193636781272986 1101 80-81 0.5780869294619713 1101 82-83 0.4972675539649849 1101 84-85 0.4423784821444592 1101 86-87 0.530158408742949 1101 88-89 0.4439228263434245 1101 90-91 0.5367002092822553 1101 92-93 0.7644555094661705 1101 94-95 0.5697850900151629 1101 96-97 0.7816223959731694 1101 98-99 0.43917949104485743 1101 100-101 1.1930745650644035 1102 1 0.2789726299637536 1102 2 0.09869427282819032 1102 3 -0.044097858973970006 1102 4 0.18718014252838344 1102 5 0.1706985082076784 1102 6 0.10677599144538874 1102 7 0.07073169570094251 1102 8 -0.01918163338409329 1102 9 0.20900333145563366 1102 10-11 0.05466761997325165 1102 12-13 0.22125599229303106 1102 14-15 0.18843413806452958 1102 16-17 0.3996987955635305 1102 18-19 0.3762189962147673 1102 20-21 0.4278319227331977 1102 22-23 0.5022074018338216 1102 24-25 0.5848635590402012 1102 26-27 0.4445531190712657 1102 28-29 0.5637122912846522 1102 30-31 0.336929488092089 1102 32-33 0.40565710904878216 1102 34-35 0.461117243483983 1102 36-37 0.7236913919983792 1102 38-39 0.6637487951433059 1102 40-41 0.6418067631102886 1102 42-43 0.4536494026885549 1102 44-45 0.3218048492002339 1102 46-47 0.25831219471724154 1102 48-49 0.04510065788963402 1102 50-51 0.047360666742903845 1102 52-53 0.038654292577597005 1102 54-55 0.10793323482665329 1102 56-57 0.1173553132918812 1102 58-59 -0.08583558238553479 1102 60-61 0.4098680816822977 1102 62-63 0.4766906550193539 1102 64-65 0.5729178212209689 1102 66-67 0.35635190134232175 1102 68-69 0.22467155090838276 1102 70-71 0.13790520200029732 1102 72-73 0.1846645202246826 1102 74-75 0.22996771641030733 1102 76-77 0.036953613054574674 1102 78-79 0.01010876967675145 1102 80-81 0.2974149854640906 1102 82-83 0.24715563113069194 1102 84-85 0.4511288128255586 1102 86-87 0.5517289913521743 1102 88-89 0.6822879389784937 1102 90-91 -0.027909790514247135 1102 92-93 0.5269349054898562 1102 94-95 0.7967442483554485 1102 96-97 0.4929632315280017 1102 98-99 0.7473793648772968 1102 100-101 0.966496961841024 1103 1 0.03333680715485343 1103 2 -0.06639831883705938 1103 3 0.024527137270283106 1103 4 -0.03887572294578945 1103 5 0.01848562249253405 1103 6 -0.06087050769829716 1103 7 0.05180273439013661 1103 8 -0.12697655962124088 1103 9 -0.12516795060592045 1103 10-11 -0.010617265902141071 1103 12-13 -0.13637117727641623 1103 14-15 0.04765875172343215 1103 16-17 -0.24600913757416976 1103 18-19 -0.09432593753386698 1103 20-21 -0.4702471382300786 1103 22-23 -0.12353498401652985 1103 24-25 -0.1633161559236882 1103 26-27 -0.15926572375826709 1103 28-29 -0.06350234711209168 1103 30-31 -0.33688144263192044 1103 32-33 -0.24429231356782566 1103 34-35 -0.01844148136503776 1103 36-37 -0.29103614112730014 1103 38-39 -0.4308108811614346 1103 40-41 -0.3488339171304844 1103 42-43 -0.08741342343541447 1103 44-45 -0.43384128939180755 1103 46-47 -0.8159664516116436 1103 48-49 -0.9235664027130852 1103 50-51 -0.8387951516592551 1103 52-53 -0.685558753382729 1103 54-55 -0.7624184031794954 1103 56-57 -0.929556461723891 1103 58-59 -1.0005400802672995 1103 60-61 -0.6492123113189905 1103 62-63 -0.9890519311374604 1103 64-65 -1.0572863912240678 1103 66-67 -0.9537225125108719 1103 68-69 -0.8266970430403617 1103 70-71 -1.1876378789135025 1103 72-73 -0.9934229536102883 1103 74-75 -0.992427548344768 1103 76-77 -0.6293257944388877 1103 78-79 -0.9068091146863217 1103 80-81 -0.9164221854073453 1103 82-83 -0.5954435456097222 1103 84-85 -0.7055659301311614 1103 86-87 -0.9784872394810016 1103 88-89 -0.6000941055496334 1103 90-91 -0.5491472838790976 1103 92-93 -0.4715327500230799 1103 94-95 -1.0515035708078706 1103 96-97 -1.3037958098052371 1103 98-99 -1.0634311752490966 1103 100-101 -0.17810427805601847 1104 1 0.09433471729069964 1104 2 0.07127148262584626 1104 3 0.12827396515504574 1104 4 0.043543294818050526 1104 5 0.20007056054299 1104 6 0.12809426316302108 1104 7 -0.09934855684736021 1104 8 0.240792883579239 1104 9 0.3499682637544339 1104 10-11 0.11047356855076629 1104 12-13 0.21931623875583028 1104 14-15 0.15030466902452133 1104 16-17 0.19009606469482065 1104 18-19 0.12974668524564947 1104 20-21 0.16210470532866594 1104 22-23 0.1799991255520652 1104 24-25 -0.03413176273067364 1104 26-27 0.21205368958700888 1104 28-29 0.24805068319093948 1104 30-31 -0.03348261444860867 1104 32-33 0.1461437589803154 1104 34-35 0.061304562820659214 1104 36-37 -0.07420340489801447 1104 38-39 -0.2639716141066515 1104 40-41 -0.2995774982619963 1104 42-43 0.16035676614512084 1104 44-45 0.4561422902469445 1104 46-47 0.47731892680305066 1104 48-49 0.3850757453687166 1104 50-51 -0.01599510687645278 1104 52-53 0.3010297299730027 1104 54-55 0.30001162369018175 1104 56-57 0.5741054654294118 1104 58-59 0.2213633754723645 1104 60-61 0.5262009966374208 1104 62-63 0.327805062443673 1104 64-65 -0.39871328329751776 1104 66-67 -0.18730681613828182 1104 68-69 -0.33529440831875945 1104 70-71 -0.22370669515473196 1104 72-73 0.030335256570335645 1104 74-75 0.03541709264642634 1104 76-77 -0.5907705996336965 1104 78-79 -0.11882303467438149 1104 80-81 -0.0031422182480689287 1104 82-83 0.36860892490826913 1104 84-85 0.050251169435934884 1104 86-87 -0.385124424135423 1104 88-89 0.03355923823301765 1104 90-91 -0.17191747345963293 1104 92-93 -0.06421449356689024 1104 94-95 -0.09343147049439082 1104 96-97 0.23908372445360726 1104 98-99 0.40863088625262023 1104 100-101 0.9719834216006475 1105 1 0.002565801135276047 1105 2 -0.023309294470266195 1105 3 0.04618223275656419 1105 4 0.004944112813966228 1105 5 -0.035450181496159416 1105 6 0.01770133298481369 1105 7 0.05820695001796139 1105 8 0.18121429754359752 1105 9 0.12627924827094006 1105 10-11 -0.07019762630754656 1105 12-13 -0.028273594723579265 1105 14-15 -6.228799091658743E-4 1105 16-17 -0.11551122423301763 1105 18-19 -0.06951200596089535 1105 20-21 -0.13087659762196324 1105 22-23 -0.08493660334072217 1105 24-25 4.048279792314702E-4 1105 26-27 -0.23210165660054827 1105 28-29 -0.13851629022419587 1105 30-31 0.026452018913936115 1105 32-33 0.021280700259893592 1105 34-35 -0.10955250700113339 1105 36-37 -0.36249218666839766 1105 38-39 -0.21285599914901354 1105 40-41 -0.2808348017969067 1105 42-43 -0.370117233270598 1105 44-45 -0.45716475036071813 1105 46-47 -0.538484096860401 1105 48-49 -0.12287597826551888 1105 50-51 -0.33243901572833323 1105 52-53 -0.0941736734742662 1105 54-55 -0.011365457817262836 1105 56-57 -0.22699189069095382 1105 58-59 -0.5581155173117409 1105 60-61 -0.523161769941602 1105 62-63 -0.6947993781055573 1105 64-65 -0.20083313430540528 1105 66-67 0.11919515581614348 1105 68-69 -0.05346012552588775 1105 70-71 -0.17524774102093232 1105 72-73 -0.35215999613197013 1105 74-75 -0.3629633484870851 1105 76-77 -0.43495881172835027 1105 78-79 -0.5885407530500721 1105 80-81 -0.9719013476667051 1105 82-83 -0.46463311423867637 1105 84-85 -0.12009465294209676 1105 86-87 -0.15701677003083958 1105 88-89 -0.37141264315757283 1105 90-91 -0.12840666422796332 1105 92-93 -0.26889605944477424 1105 94-95 0.2677648485240134 1105 96-97 0.1442108059610554 1105 98-99 0.514030024435499 1105 100-101 1.0903919813435614 1106 1 -0.008735458147896225 1106 2 -0.20607629859190268 1106 3 -0.26240862366996254 1106 4 -0.10648250497554557 1106 5 -0.07365603535853182 1106 6 0.03309573743694472 1106 7 -0.09664879273118743 1106 8 -0.09960775035854397 1106 9 -0.012878190616788743 1106 10-11 -0.09871668649862642 1106 12-13 -0.028914632540057994 1106 14-15 -0.24091234409652174 1106 16-17 -0.1373539942761326 1106 18-19 -0.21048809287612613 1106 20-21 0.055864840961866946 1106 22-23 0.12185744782834007 1106 24-25 0.06799395597786173 1106 26-27 -0.0818566377017973 1106 28-29 0.05864794841281196 1106 30-31 0.06682790449580978 1106 32-33 0.08556492218243505 1106 34-35 -0.010395827861920282 1106 36-37 -0.23759902630447272 1106 38-39 -0.2478329217876194 1106 40-41 -0.11368537825610048 1106 42-43 0.2245521811532285 1106 44-45 -0.3311936967794509 1106 46-47 -0.04093903427612133 1106 48-49 -0.5710231415544698 1106 50-51 -0.6522456597291679 1106 52-53 -0.5264848284819621 1106 54-55 -0.3071450547706718 1106 56-57 -0.45160679315823415 1106 58-59 -0.6332593946883307 1106 60-61 -0.5011053206263298 1106 62-63 -0.3766214450014118 1106 64-65 -0.2513089268180835 1106 66-67 -0.392428996589409 1106 68-69 -0.23501153201034697 1106 70-71 -0.16678129341509162 1106 72-73 -0.036232818027922775 1106 74-75 -0.0519935294971674 1106 76-77 -0.14128751692692632 1106 78-79 0.1783399094506386 1106 80-81 0.14512366705342217 1106 82-83 -0.22698852459785002 1106 84-85 -0.34601062475542577 1106 86-87 -0.19047419562296497 1106 88-89 0.046168887355825916 1106 90-91 -0.04996737763182679 1106 92-93 0.3003550184484425 1106 94-95 -0.1325181578008916 1106 96-97 -0.28291981380380093 1106 98-99 0.1413496316455145 1106 100-101 0.8177467269227776 1107 1 -0.29779923909559614 1107 2 -0.14913350179782725 1107 3 -0.10893119728376632 1107 4 -0.23488349645920437 1107 5 -0.24594078792163288 1107 6 -0.33135166474085054 1107 7 -0.29391353237028284 1107 8 -0.22535642661213018 1107 9 -0.4175829155958084 1107 10-11 -0.20213769090629086 1107 12-13 -0.3751430402828859 1107 14-15 -0.08428589617351889 1107 16-17 -0.19679478977780462 1107 18-19 -0.2851676449724181 1107 20-21 -0.1759571549294634 1107 22-23 -0.1675197885556372 1107 24-25 -0.3919560840479903 1107 26-27 -0.2854290651971496 1107 28-29 -0.2559407498308701 1107 30-31 -0.245918640260804 1107 32-33 -0.3563734862355261 1107 34-35 -0.20598237531907415 1107 36-37 -0.0834657546977482 1107 38-39 -0.009312624342527442 1107 40-41 -0.023361988693686442 1107 42-43 -0.2108325974507892 1107 44-45 -0.34559335728310003 1107 46-47 -0.7212096668774137 1107 48-49 -0.5250127058996412 1107 50-51 -0.5494563885853978 1107 52-53 -0.32125832076130934 1107 54-55 0.09436242475827328 1107 56-57 -0.16650701788167055 1107 58-59 -0.08756241446977953 1107 60-61 -0.5941678284627159 1107 62-63 -0.5173774030659004 1107 64-65 -0.33948152984847013 1107 66-67 -0.1744424414164314 1107 68-69 -0.5547119650610881 1107 70-71 -0.42636579617832027 1107 72-73 -0.30312324365218046 1107 74-75 -0.33002850147907736 1107 76-77 -0.5317613650987596 1107 78-79 -0.3367107738822135 1107 80-81 -0.3457379013811348 1107 82-83 -0.45757598830935464 1107 84-85 -0.315166609825301 1107 86-87 -0.012711206511927031 1107 88-89 -0.432270201019314 1107 90-91 -0.19364088244939737 1107 92-93 -0.24050510326871688 1107 94-95 -0.5354096636408094 1107 96-97 -0.037841064153422366 1107 98-99 -0.2610258783223678 1107 100-101 0.9078145297448401 1108 1 0.04808089466072829 1108 2 -0.204722401166201 1108 3 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-0.40568329604468545 2316 64-65 -0.571350612184844 2316 66-67 -0.673497146078617 2316 68-69 -1.0121583664327183 2316 70-71 -0.8173506637986989 2316 72-73 -0.6904914955064143 2316 74-75 -1.2474050964257657 2316 76-77 -0.7583184234315183 2316 78-79 -0.570030329631674 2316 80-81 -0.9843710119768616 2316 82-83 -1.1894450706457285 2316 84-85 -1.1797674043326403 2316 86-87 -1.1636943902053893 2316 88-89 -1.4223426864189044 2316 90-91 -1.631033245075404 2316 92-93 -2.128696183048582 2316 94-95 -2.2350162370791544 2316 96-97 -2.6346438992740175 2316 98-99 -3.117061608189875 2316 100-101 -4.160862898745673 >>END_MODULE >>Per sequence quality scores pass #Quality Count 3 1.0 4 5.0 5 23.0 6 54.0 7 95.0 8 188.0 9 321.0 10 513.0 11 803.0 12 1084.0 13 1489.0 14 1784.0 15 2475.0 16 3453.0 17 4772.0 18 4437.0 19 2530.0 20 1939.0 21 2108.0 22 2397.0 23 2500.0 24 2828.0 25 3229.0 26 3734.0 27 4480.0 28 5460.0 29 6579.0 30 7783.0 31 9526.0 32 11742.0 33 14886.0 34 19931.0 35 29093.0 36 41728.0 37 72065.0 38 66202.0 39 5191.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 38.971124235707165 23.731980541216025 17.100522173790146 20.19637304928666 2 14.774114774114775 23.81693279751532 40.271998767144396 21.136953661225505 3 17.383560344725392 29.007077065329494 33.183079056865466 20.42628353307965 4 11.432068470903422 20.862821105539552 40.45633438837322 27.248776035183802 5 10.828976848394325 39.97386109036595 36.41606505684176 12.781097004397976 6 25.24212572756262 40.870941356378246 18.84283461953365 15.044098296525481 7 23.147753002121934 36.168901217444905 24.285180838578896 16.39816494185426 8 23.201394075180485 35.671906302974264 22.267861588249936 18.858838033595315 9 24.70334412081985 17.9318254560973 24.18975307324822 33.17507734983463 10-11 22.190363573858722 28.716200196782722 29.25824175824176 19.8351944711168 12-13 24.119367687328854 27.766071576751187 29.3138091681781 18.80075156774186 14-15 21.583314810698674 28.968215158924203 28.950729791805585 20.497740238571534 16-17 18.080851637645015 31.574845037571812 30.423166345850355 19.921136978932815 18-19 17.977316642365185 30.12301883661107 33.346965871237714 18.552698649786027 20-21 19.21283947982977 28.812813400192038 33.352300342591604 18.622046777386583 22-23 19.21032042391266 28.730277273966593 33.20249060540323 18.85691169671752 24-25 19.238326398520574 29.47621418495205 33.06927700131584 18.21618241521154 26-27 18.69776663465984 28.985887359673768 34.11409248787889 18.202253517787497 28-29 18.080301575447205 29.08753867491732 34.28627736880164 18.545882380833838 30-31 18.587520893346134 28.872233483883967 33.68822385812677 18.852021764643123 32-33 18.346876963381817 29.36863567931529 33.62835923515535 18.65612812214754 34-35 19.15298441111967 28.852468733329385 33.4114753126667 18.583071542884237 36-37 19.054118135206423 28.754186301532258 33.2702053881035 18.92149017515782 38-39 18.31206958129995 29.515589548578124 33.408864235549714 18.763476634572207 40-41 18.37805237662686 29.10632547125776 33.78296877940499 18.73265337271039 42-43 18.37780728218928 29.2264081606041 32.78822615921713 19.607558397989486 44-45 18.50916122722659 29.18819598284076 32.5779611916634 19.72468159826926 46-47 19.515451467352246 29.43210022893807 31.93204466144579 19.120403642263895 48-49 18.94795022330756 29.515717473705422 31.7289369256165 19.80739537737051 50-51 18.321943181616117 29.794236365899486 32.09637075981104 19.787449692673356 52-53 18.768003840819375 30.417452019393764 31.221623575992506 19.59292056379435 54-55 18.780154581125455 29.928310632194126 31.051365031947558 20.24016975473286 56-57 19.22617565821449 29.94787036049172 30.50398899913463 20.32196498215916 58-59 19.19565062768946 29.576828241876786 31.26444752658345 19.963073603850304 60-61 19.23560800608147 30.42260575516754 30.80210480456762 19.539681434183365 62-63 18.69687755610086 30.226892848252074 31.45797029292175 19.61825930272532 64-65 18.71898407806121 30.35022338131895 30.956427677056553 19.974364863563284 66-67 18.78004398053476 30.27810871845938 30.004712200152923 20.937135100852938 68-69 18.785554566024988 29.739361599660956 30.831171102019283 20.64391273229477 70-71 19.263220598172055 29.734936045615655 30.628756356911698 20.37308699930059 72-73 19.03532012755314 29.02634043410742 31.080408264874283 20.857931173465154 74-75 18.388219116374458 30.660763185035027 30.02670199272141 20.924315705869105 76-77 18.492677166288853 29.77897978802948 30.711988321445105 21.01635472423656 78-79 18.56828503900838 29.244208015055083 30.699631772751186 21.48787517318535 80-81 18.639828348566212 29.737158742013108 30.11234989390329 21.510663015517384 82-83 18.485869578102587 29.442132840191093 30.59467501215074 21.47732256955558 84-85 18.247151985016064 29.202970707825077 30.299649110328726 22.25022819683014 86-87 17.91729611173602 29.606128148585643 30.610321810902335 21.866253928776004 88-89 18.352357039416205 28.96748084854312 30.33617560252485 22.343986509515823 90-91 18.210839342799794 28.570663891160507 30.429559140584345 22.788937625455358 92-93 18.579074647035814 29.01063337956542 30.349733869151347 22.060558104247423 94-95 18.057630072193177 28.202016430171774 30.749671040933173 22.990682456701876 96-97 17.79765371125267 28.255634930866425 30.702390001970798 23.244321355910103 98-99 17.819208838626313 28.525789205400855 30.11146081534431 23.54354114062852 100-101 17.793330112883677 27.80053167055393 31.0391580993815 23.3669801171809 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 2376.0 1 1427.0 2 453.0 3 389.5 4 299.0 5 304.0 6 503.0 7 932.5 8 1333.5 9 1357.0 10 1071.5 11 789.0 12 738.0 13 763.5 14 749.0 15 738.5 16 761.0 17 793.5 18 844.5 19 922.0 20 1052.5 21 1210.0 22 1347.5 23 1495.5 24 1667.5 25 2003.0 26 2647.5 27 3351.0 28 3603.0 29 4045.0 30 5162.0 31 6218.0 32 7564.0 33 9582.5 34 11594.0 35 13201.0 36 15795.0 37 18059.0 38 17672.5 39 16537.5 40 15302.5 41 13723.0 42 12338.0 43 11981.5 44 11926.5 45 11698.0 46 11341.0 47 11657.0 48 13569.0 49 12359.0 50 8751.0 51 6754.0 52 5488.5 53 4426.5 54 3715.0 55 3394.5 56 3049.0 57 2706.0 58 2563.5 59 2311.0 60 1992.5 61 1764.0 62 1430.0 63 926.5 64 537.5 65 402.5 66 301.0 67 206.5 68 157.5 69 121.0 70 74.0 71 57.0 72 50.0 73 47.5 74 43.0 75 41.5 76 31.5 77 15.5 78 5.5 79 1.0 80 1.5 81 1.5 82 0.0 83 0.0 84 0.0 85 0.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.3950472397074339 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 8.890785589814716E-4 16-17 0.002222696397453679 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0023708761572839242 36-37 0.00533447135388883 38-39 0.011409841506928885 40-41 0.0019263368777931887 42-43 5.927190393209811E-4 44-45 1.4817975983024526E-4 46-47 1.4817975983024526E-4 48-49 2.9635951966049053E-4 50-51 5.927190393209811E-4 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.00281541543677466 62-63 0.0 64-65 1.4817975983024526E-4 66-67 0.0017781571179629433 68-69 0.0019263368777931887 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.11854380786419622 78-79 1.4817975983024526E-4 80-81 0.0 82-83 0.0 84-85 0.0 86-87 0.14551252415330085 88-89 0.4408347854949797 90-91 0.34170252616854563 92-93 0.0 94-95 0.0 96-97 4.445392794907358E-4 98-99 0.0 100-101 2.9635951966049053E-4 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 337428.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 39.98722920341204 #Duplication Level Percentage of deduplicated Percentage of total 1 77.38829436317616 30.94543464361445 2 10.948996574396027 8.756400711354944 3 3.7104680717623926 4.451140117125154 4 1.7566901618630129 2.8098068856718115 5 1.0327145491475658 2.064769668923102 6 0.7295622238779828 1.7503903118615942 7 0.5508749189887207 1.5419573153609107 8 0.42308566134827524 1.3534418650408517 9 0.31395217420487254 1.1298669793955805 >10 2.67853029499737 22.06895351640678 >50 0.31839507420653873 8.683400636600526 >100 0.13730323712869386 8.940112812363862 >500 0.005937437281240816 1.39574261573985 >1k 0.005195257621085713 4.108581920540609 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2782 0.8244721836954847 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2603 0.7714238296762569 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 1970 0.5838282537311663 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 1862 0.5518214256078333 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1819 0.5390779662624323 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1606 0.4759533885747478 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 1202 0.3562241426319096 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 802 0.23768033476771344 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 651 0.19293004729897933 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 569 0.1686285666868191 No Hit GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA 569 0.1686285666868191 No Hit ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC 539 0.1597377810970044 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 531 0.1573669049397205 No Hit TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC 524 0.15529238830209705 No Hit CTTATACACATCTCCGAGCCCACGAGACGCTACGCTATCTCGTATGCCGT 518 0.1535142311841341 No Hit GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG 487 0.1443270860746589 No Hit GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT 485 0.1437343670353379 No Hit TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT 437 0.12950911009163435 No Hit ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC 427 0.12654551489502947 No Hit GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA 394 0.11676565074623328 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 1.4817975983024526E-4 0.0 16-17 0.0 0.0 0.0 2.9635951966049053E-4 0.0 18-19 0.0 0.0 0.0 2.9635951966049053E-4 0.0 20-21 0.0 0.0 0.0 2.9635951966049053E-4 0.0 22-23 0.0 0.0 0.0 8.890785589814715E-4 0.0 24-25 0.0 0.0 0.0 0.0025190559171141694 0.0 26-27 0.0 0.0 0.0 0.0069644487120215275 0.0 28-29 0.0 0.0 0.0 0.02563509845063243 0.0 30-31 0.0 0.0 0.0 0.06682907168344061 0.0 32-33 0.0 0.0 0.0 0.13113908744976707 0.0 34-35 0.0 0.0 0.0 0.23323494197280606 0.0 36-37 0.0 0.0 0.0 0.36155861398579847 0.0 38-39 0.0 0.0 0.0 0.5325580568299014 0.0 40-41 0.0 0.0 0.0 0.7093365103073841 0.0 42-43 0.0 0.0 0.0 0.8868558625840179 0.0 44-45 0.0 0.0 0.0 1.1116445582465 0.0 46-47 0.0 0.0 0.0 1.3685882617921452 0.0 48-49 0.0 0.0 0.0 1.620197493983902 0.0 50-51 0.0 0.0 0.0 1.8697322095380349 0.0 52-53 0.0 0.0 0.0 2.104152589589483 0.0 54-55 0.0 0.0 0.0 2.389398627262705 0.0 56-57 0.0 0.0 0.0 2.7509572412485035 0.0 58-59 0.0 0.0 0.0 3.1171094277890394 0.0 60-61 0.0 0.0 0.0 3.509044892540038 0.0 62-63 0.0 0.0 0.0 3.9063148286449256 0.0 64-65 0.0 0.0 0.0 4.3422596820655075 0.0 66-67 0.0 0.0 0.0 4.839254596536151 0.0 68-69 0.0 0.0 0.0 5.383667034152471 0.0 70-71 0.0 0.0 0.0 5.980683286508529 0.0 72-73 0.0 0.0 0.0 6.583478549497967 0.0 74-75 0.0 0.0 0.0 7.202721765828562 0.0 76-77 0.0 0.0 0.0 7.887015896724634 0.0 78-79 0.0 0.0 0.0 8.697855542515736 0.0 80-81 0.0 0.0 0.0 9.52869945588392 0.0 82-83 0.0 0.0 0.0 10.339687281434855 0.0 84-85 0.0 0.0 0.0 11.171864812641513 0.0 86-87 0.0 0.0 0.0 12.051608046753678 0.0 88-89 0.0 0.0 0.0 12.972545254098652 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CGTCACT 25 0.004973068 57.130245 1 GATCTAC 60 8.167662E-5 39.673775 1 GCATGGC 50 0.0016220137 37.962654 3 CGGGTCG 55 7.066228E-8 34.511505 14-15 GTCACTA 60 0.0039031615 31.73902 1 CGCAGAA 160 1.9153958E-9 29.658325 9 ACAGTGC 65 0.0058664153 29.202044 8 GAGTGAT 115 3.8835497E-6 28.884628 7 GTATAGT 150 3.249079E-8 28.56512 1 GGCATGG 70 0.00842362 27.116182 2 TAGATAT 130 1.0070242E-5 25.551788 7 GTAATGC 100 0.0016607101 23.72666 6 GTAGATA 140 1.7882072E-5 23.72666 6 CCCGGGT 80 1.8736155E-6 23.72666 12-13 GAGTACT 2135 0.0 22.893171 12-13 ATACTCA 125 2.2663566E-4 22.777594 6 CTCCCCG 105 0.002205596 22.59682 9 ATAGTAA 190 3.3038668E-7 22.477888 3 GTACTTT 2210 0.0 22.116253 14-15 GGTCCTA 130 2.955131E-4 21.901531 8 >>END_MODULE