##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938883_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1191291 Sequences flagged as poor quality 0 Sequence length 101 %GC 40 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.00172334047684 33.0 31.0 34.0 30.0 34.0 2 32.06546175535616 33.0 31.0 34.0 30.0 34.0 3 32.095167343663306 34.0 31.0 34.0 30.0 34.0 4 35.620887759581834 37.0 35.0 37.0 33.0 37.0 5 35.68008236442649 37.0 35.0 37.0 35.0 37.0 6 35.81378353399799 37.0 35.0 37.0 35.0 37.0 7 35.766896585301154 37.0 35.0 37.0 35.0 37.0 8 35.76784177837321 37.0 35.0 37.0 35.0 37.0 9 37.37482529457538 39.0 37.0 39.0 35.0 39.0 10-11 37.2745571820823 39.0 37.0 39.0 34.0 39.0 12-13 37.21174129578751 39.0 37.0 39.0 34.0 39.0 14-15 38.336651162478354 40.0 38.0 41.0 34.0 41.0 16-17 38.31209670852881 40.0 38.0 41.0 34.0 41.0 18-19 38.311156971722276 40.0 38.0 41.0 34.0 41.0 20-21 38.421292110827665 40.0 38.0 41.0 34.0 41.0 22-23 38.36829204619191 40.0 38.0 41.0 34.0 41.0 24-25 38.25806792798737 40.0 38.0 41.0 34.0 41.0 26-27 38.11031771414373 40.0 38.0 41.0 34.0 41.0 28-29 38.0194885212765 40.0 38.0 41.0 33.5 41.0 30-31 37.84124743660449 40.0 37.5 41.0 33.0 41.0 32-33 37.56788685552061 40.0 36.5 41.0 33.0 41.0 34-35 37.21031217393568 40.0 36.0 41.0 32.5 41.0 36-37 36.913033843116416 39.5 35.5 41.0 31.0 41.0 38-39 36.68315843903798 39.0 35.0 41.0 30.5 41.0 40-41 36.640232319391316 39.0 35.0 41.0 31.0 41.0 42-43 36.404654278425674 39.0 35.0 41.0 30.0 41.0 44-45 36.24758602222295 39.0 35.0 41.0 30.0 41.0 46-47 35.949122002936306 39.0 35.0 41.0 29.0 41.0 48-49 35.622917490352904 39.0 35.0 41.0 27.0 41.0 50-51 34.478641238790516 38.0 34.0 40.0 25.0 40.5 52-53 34.67254978002856 38.0 34.5 39.5 25.5 40.5 54-55 35.23358860261683 39.0 35.0 40.5 26.0 41.0 56-57 35.11726815698263 39.0 35.0 41.0 26.0 41.0 58-59 34.949993326567565 39.0 35.0 40.5 26.0 41.0 60-61 34.56972435786051 38.0 34.5 40.0 24.5 41.0 62-63 34.27779148839369 38.0 34.0 40.0 23.0 41.0 64-65 33.925952181289034 37.5 34.0 40.0 22.5 41.0 66-67 33.42884777942585 37.0 33.5 39.5 21.5 41.0 68-69 32.9800300682201 36.0 33.0 39.0 20.0 41.0 70-71 32.43228690555037 35.5 32.5 38.5 18.0 40.0 72-73 31.968022086962797 35.0 32.0 37.5 14.5 40.0 74-75 31.528115716479014 35.0 31.0 37.0 10.0 39.5 76-77 31.110330305525686 35.0 31.0 36.5 7.0 39.0 78-79 30.678199533111556 35.0 31.0 36.0 2.0 39.0 80-81 30.16454837650918 35.0 30.5 36.0 2.0 37.5 82-83 29.660126283166747 34.0 30.0 35.0 2.0 37.0 84-85 29.204265792321102 34.0 29.0 35.0 2.0 37.0 86-87 28.867008564658008 34.0 29.0 35.0 2.0 36.0 88-89 28.338565472248174 34.0 29.0 35.0 2.0 36.0 90-91 27.829648675260707 34.0 27.5 35.0 2.0 35.5 92-93 27.323070517614923 34.0 26.5 35.0 2.0 35.0 94-95 26.76553293863548 33.5 25.0 35.0 2.0 35.0 96-97 26.11208428503195 33.0 24.5 35.0 2.0 35.0 98-99 25.37530502622785 33.0 20.0 35.0 2.0 35.0 100-101 22.218606536941856 28.5 2.0 33.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.10353215407857164 1101 2 0.23671780598297687 1101 3 0.32121526835827297 1101 4 0.1995762761281199 1101 5 0.23029085700629537 1101 6 0.2680006066412517 1101 7 0.2720841597204 1101 8 0.18774388823116084 1101 9 0.30043073345660076 1101 10-11 0.22566683848547342 1101 12-13 0.4151316605133957 1101 14-15 0.4582232273479221 1101 16-17 0.4162330068230631 1101 18-19 0.5149712575419585 1101 20-21 0.47973195600572893 1101 22-23 0.5295941612619757 1101 24-25 0.4510777575950371 1101 26-27 0.5668128082957935 1101 28-29 0.3901037058134236 1101 30-31 0.417668553276485 1101 32-33 0.4871337453555 1101 34-35 0.22709636642856879 1101 36-37 0.18365007996104765 1101 38-39 0.4398471270164208 1101 40-41 0.4311359163693069 1101 42-43 0.47147893125175955 1101 44-45 0.44852854174013856 1101 46-47 0.35288659519607535 1101 48-49 0.30614671235836255 1101 50-51 0.16076760013059044 1101 52-53 0.1345299807898641 1101 54-55 0.3145157141399366 1101 56-57 0.35467646461466273 1101 58-59 0.3451576841501307 1101 60-61 0.288574094823133 1101 62-63 0.2608574791910101 1101 64-65 0.28654692912449065 1101 66-67 0.5290887855314494 1101 68-69 0.329101089801874 1101 70-71 0.2575676142627117 1101 72-73 -0.031143856375472012 1101 74-75 -0.014269035021651888 1101 76-77 -0.12138693458974714 1101 78-79 -0.03887082652780194 1101 80-81 -0.14531632396304772 1101 82-83 -0.058502257207646124 1101 84-85 0.009646923242065952 1101 86-87 0.15794510860433775 1101 88-89 0.09654422758920944 1101 90-91 -0.001275664390224307 1101 92-93 0.15460082529389396 1101 94-95 0.01658760674641968 1101 96-97 -0.1871112605831371 1101 98-99 -0.35969285634592296 1101 100-101 -0.09926071902940592 1102 1 0.2851168032609124 1102 2 0.3245985894765866 1102 3 0.2832747459063114 1102 4 0.10674880525609609 1102 5 0.32972815455993043 1102 6 0.3867420500618408 1102 7 0.4096194961940114 1102 8 0.36401738373943004 1102 9 0.3110966091895193 1102 10-11 0.46223101890550566 1102 12-13 0.36144996442969557 1102 14-15 0.34507822673629107 1102 16-17 0.44724029112185804 1102 18-19 0.5392786459021579 1102 20-21 0.3629502520461969 1102 22-23 0.40692486038373943 1102 24-25 0.33304178686629626 1102 26-27 0.37068050786832174 1102 28-29 0.4612894194049133 1102 30-31 0.3009580668689793 1102 32-33 0.31053935193634175 1102 34-35 0.44355415620441363 1102 36-37 0.392097989767791 1102 38-39 0.44210430448887905 1102 40-41 0.39744456821266994 1102 42-43 0.2117535461323783 1102 44-45 0.48061246785486134 1102 46-47 0.6684130269621207 1102 48-49 0.68191481460331 1102 50-51 0.6549570857387863 1102 52-53 0.6319196480447005 1102 54-55 0.9053733913082596 1102 56-57 0.8421794599411143 1102 58-59 0.7346489897438104 1102 60-61 0.8120637381153628 1102 62-63 0.7586652353689161 1102 64-65 0.6641000783646476 1102 66-67 1.0067478394607292 1102 68-69 1.0176357342084472 1102 70-71 0.7355249440569551 1102 72-73 0.6913536007389922 1102 74-75 0.6961000729826985 1102 76-77 0.6140415353388491 1102 78-79 0.9554238097013226 1102 80-81 0.7814045853426599 1102 82-83 0.8308973034219527 1102 84-85 0.7121490053081452 1102 86-87 0.42973977415833176 1102 88-89 0.33383859738090393 1102 90-91 0.6227279182715719 1102 92-93 0.7261644108379066 1102 94-95 0.6241638137163541 1102 96-97 0.370587524361639 1102 98-99 0.27137969674467044 1102 100-101 0.3043023369911566 1103 1 0.2354109638134645 1103 2 0.30568579878381996 1103 3 0.3258240980110543 1103 4 0.3579527259351991 1103 5 0.1903457188582749 1103 6 0.3254544557361001 1103 7 0.2696265624558123 1103 8 0.07477538788305793 1103 9 0.39010298147358213 1103 10-11 0.3160357118140311 1103 12-13 0.36751362655782316 1103 14-15 0.5308488160777074 1103 16-17 0.5204688560214237 1103 18-19 0.6992021481371324 1103 20-21 0.5098884441793743 1103 22-23 0.5694332821366928 1103 24-25 0.5708897981520735 1103 26-27 0.6593575400720937 1103 28-29 0.5959742244966648 1103 30-31 0.5780676790931096 1103 32-33 0.44832808420006387 1103 34-35 0.4817857525134315 1103 36-37 0.2496512586727775 1103 38-39 0.42952922545721606 1103 40-41 0.41544076862129486 1103 42-43 0.1969392716089473 1103 44-45 0.3021762872452527 1103 46-47 0.3354997623744609 1103 48-49 0.238295657444759 1103 50-51 0.14141819558035706 1103 52-53 0.2620725170903455 1103 54-55 0.38582944346030956 1103 56-57 0.2064313426614106 1103 58-59 0.12422937353121455 1103 60-61 0.33017335286917415 1103 62-63 0.2495933525980334 1103 64-65 0.004095626113773676 1103 66-67 0.28402651916597677 1103 68-69 0.32360655678709094 1103 70-71 0.17243115250155938 1103 72-73 0.29691073500724485 1103 74-75 0.12590249591785962 1103 76-77 0.2798608970648395 1103 78-79 0.1670360051585078 1103 80-81 0.09503193025802759 1103 82-83 0.18147284960267385 1103 84-85 0.09000175554277234 1103 86-87 0.29798223969344306 1103 88-89 0.46147822140180494 1103 90-91 0.42256762037551354 1103 92-93 0.4207475899305386 1103 94-95 0.2809028603781343 1103 96-97 0.11765751323944329 1103 98-99 -0.008361212610708435 1103 100-101 0.2564355807173193 1104 1 0.24926478377992112 1104 2 0.25698253848292296 1104 3 0.27905237196988963 1104 4 0.2689211461352272 1104 5 0.15086043100016155 1104 6 0.2228500665166564 1104 7 0.15711489845185866 1104 8 0.18629277045164372 1104 9 0.2944850103531991 1104 10-11 0.2918117761621346 1104 12-13 0.3406068666838138 1104 14-15 0.4798540800664526 1104 16-17 0.3750202764057633 1104 18-19 0.3874490473097296 1104 20-21 0.30970644852512663 1104 22-23 0.40546569422378553 1104 24-25 0.37200210401717726 1104 26-27 0.3749244499230855 1104 28-29 0.3983283490289722 1104 30-31 0.423407325796731 1104 32-33 0.3676189623497592 1104 34-35 0.5072680368646942 1104 36-37 0.5631966282388419 1104 38-39 0.6062551054541387 1104 40-41 0.5574862278627819 1104 42-43 0.3274947054851012 1104 44-45 0.4806518711479768 1104 46-47 0.4759909853406299 1104 48-49 0.6127653533500208 1104 50-51 0.5546504036868498 1104 52-53 0.5572869074751594 1104 54-55 0.6379942577991216 1104 56-57 0.6108389930909013 1104 58-59 0.6428339247449699 1104 60-61 0.6726739665466184 1104 62-63 0.7314462700247404 1104 64-65 0.5319028594905504 1104 66-67 0.6591921294258327 1104 68-69 0.9517907575476343 1104 70-71 0.9581630899287745 1104 72-73 0.7160565621245922 1104 74-75 0.6695260773311666 1104 76-77 0.7858170809085507 1104 78-79 0.6547285697020833 1104 80-81 0.817852631478523 1104 82-83 1.0196967307054585 1104 84-85 0.947320716564132 1104 86-87 0.7418578214472618 1104 88-89 0.8959680508862604 1104 90-91 0.9894439980503797 1104 92-93 0.9613730367579727 1104 94-95 0.9900453732240351 1104 96-97 0.922249804128338 1104 98-99 0.7746841813371503 1104 100-101 0.9839917229053512 1105 1 0.2153600492716521 1105 2 0.21324272819833112 1105 3 0.10333445885521542 1105 4 0.20609757451303778 1105 5 0.22727924638128627 1105 6 0.24329390542846596 1105 7 0.23180536423694065 1105 8 0.29074767649090916 1105 9 0.3340110300359953 1105 10-11 0.2540911586979817 1105 12-13 0.1399851508048897 1105 14-15 0.26258228029895037 1105 16-17 0.18742163917030297 1105 18-19 0.20880827137796132 1105 20-21 0.3142466261333894 1105 22-23 0.27130285318690284 1105 24-25 0.334483008796731 1105 26-27 0.25436119920333766 1105 28-29 0.18276632719976504 1105 30-31 0.32147421379541186 1105 32-33 0.469979617621064 1105 34-35 0.42369794782347014 1105 36-37 0.33554524749536085 1105 38-39 0.4476152146396828 1105 40-41 0.43167510083185334 1105 42-43 0.3458972426106328 1105 44-45 0.5837261522470101 1105 46-47 0.6341772535359098 1105 48-49 0.5319632315973948 1105 50-51 0.33601971177011336 1105 52-53 0.4647036372986264 1105 54-55 0.6445468918528974 1105 56-57 0.5793695165982697 1105 58-59 0.5768353899302241 1105 60-61 0.5070483664252308 1105 62-63 0.7324906294171356 1105 64-65 0.7319128960885735 1105 66-67 0.679534021790829 1105 68-69 0.8388375399486634 1105 70-71 0.752810738164186 1105 72-73 0.640694750668807 1105 74-75 0.5364550126851988 1105 76-77 0.5069166178815614 1105 78-79 0.5058008656094444 1105 80-81 0.5449982309044614 1105 82-83 0.3174897065093525 1105 84-85 0.4498389543271877 1105 86-87 0.5657250095955426 1105 88-89 0.5993427186709113 1105 90-91 0.4975649493302079 1105 92-93 0.9353391288623314 1105 94-95 0.739221700445583 1105 96-97 0.5451890416155862 1105 98-99 0.5129099478922043 1105 100-101 0.7411949576929899 1106 1 0.2641812739000784 1106 2 0.20852247127564283 1106 3 0.193108802371043 1106 4 0.27919256494515565 1106 5 0.2586705470372408 1106 6 0.21899360429032555 1106 7 0.14523194020836172 1106 8 0.24229415578273006 1106 9 0.29434070503121745 1106 10-11 0.23495235918684187 1106 12-13 0.33884322164058034 1106 14-15 0.20561546614636939 1106 16-17 0.31868776886288686 1106 18-19 0.5251958874131901 1106 20-21 0.36984042626622227 1106 22-23 0.340093433294804 1106 24-25 0.35314568859256923 1106 26-27 0.4101092075369408 1106 28-29 0.23852502548239585 1106 30-31 0.26971552764321416 1106 32-33 0.43307351631370494 1106 34-35 0.354786063329513 1106 36-37 0.3654441251265226 1106 38-39 0.5676148204004221 1106 40-41 0.4988148040606788 1106 42-43 0.6192570556805705 1106 44-45 0.3441511270005364 1106 46-47 0.3459913909555894 1106 48-49 0.3539073466667375 1106 50-51 0.3432801583522078 1106 52-53 0.43172021657559867 1106 54-55 0.6123913735647477 1106 56-57 0.591966977090955 1106 58-59 0.46717963661482287 1106 60-61 0.4638314044329306 1106 62-63 0.5066275487102985 1106 64-65 0.3031625435477068 1106 66-67 0.2854592861585701 1106 68-69 0.23761357869327782 1106 70-71 0.4808455464577719 1106 72-73 0.3363007267293838 1106 74-75 0.2036901673027991 1106 76-77 0.1613416502546663 1106 78-79 0.13372278500874302 1106 80-81 -0.006321864076873851 1106 82-83 -0.020643237032555106 1106 84-85 0.05262850031519761 1106 86-87 0.18967155681782444 1106 88-89 0.11640418057072566 1106 90-91 0.23142881245033564 1106 92-93 0.2766676848245915 1106 94-95 0.2938564377760997 1106 96-97 0.5028716274611824 1106 98-99 0.6900397496505093 1106 100-101 1.130132452575463 1107 1 0.006908923029115499 1107 2 0.09305800494612271 1107 3 0.07237121623553122 1107 4 0.1862933841566985 1107 5 0.11316881693343106 1107 6 0.1073028344680651 1107 7 0.1496237421780151 1107 8 0.18223737288857933 1107 9 0.16564020522345402 1107 10-11 0.07912767637253637 1107 12-13 0.15206594662801365 1107 14-15 0.31678424738435496 1107 16-17 0.22545957260700789 1107 18-19 0.23576430784319768 1107 20-21 0.07773531876319595 1107 22-23 0.10396014136566833 1107 24-25 0.1385654975417978 1107 26-27 0.3517854858914191 1107 28-29 0.3243477859591408 1107 30-31 0.37448514287995494 1107 32-33 0.3257494102006717 1107 34-35 0.3645981178647446 1107 36-37 0.2000084938604445 1107 38-39 0.475499361848982 1107 40-41 0.42813045188842835 1107 42-43 0.491886665593924 1107 44-45 0.25466557410667434 1107 46-47 0.4306363499950123 1107 48-49 0.4516700414882564 1107 50-51 0.2921393249449409 1107 52-53 0.44500356913229666 1107 54-55 0.4202744362921038 1107 56-57 0.32371807873824565 1107 58-59 0.2756046506626717 1107 60-61 0.355520991278226 1107 62-63 0.14136049166123144 1107 64-65 0.3053643594173323 1107 66-67 0.17318294274036106 1107 68-69 0.3169040882606069 1107 70-71 0.03139415202367957 1107 72-73 0.28206197240412223 1107 74-75 0.1631724670100141 1107 76-77 0.2087938136237888 1107 78-79 0.4967954197941822 1107 80-81 0.22967316741988597 1107 82-83 0.30358265241824967 1107 84-85 0.4402900506065066 1107 86-87 0.6704363514234508 1107 88-89 0.5424178439546274 1107 90-91 0.4493058124207643 1107 92-93 0.4250085596360549 1107 94-95 0.3719610129394937 1107 96-97 0.5023030591590469 1107 98-99 0.40773043074949555 1107 100-101 0.2819778966354356 1108 1 0.06964079741147344 1108 2 0.10060094744052606 1108 3 0.06954610175993281 1108 4 0.10165612282040826 1108 5 0.05721118233502409 1108 6 0.0408066553505293 1108 7 0.11180718979836257 1108 8 0.11293644727697227 1108 9 0.1970219841780434 1108 10-11 0.1873065525125739 1108 12-13 0.24368980281497699 1108 14-15 0.21226330985370367 1108 16-17 0.15091389175064762 1108 18-19 0.13590726089877592 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-1.4401816749920187 2316 58-59 -1.1644341006048862 2316 60-61 -1.2301772558607027 2316 62-63 -1.4257896604465472 2316 64-65 -1.5346707702364384 2316 66-67 -1.4505911409913494 2316 68-69 -1.6079184526636254 2316 70-71 -1.6989422958975169 2316 72-73 -1.821963451324688 2316 74-75 -1.7546676025249717 2316 76-77 -1.656255802485152 2316 78-79 -1.686460048842644 2316 80-81 -1.5909320042446033 2316 82-83 -1.296444509685184 2316 84-85 -1.2938382269539765 2316 86-87 -1.4647548697847235 2316 88-89 -1.7525245723860934 2316 90-91 -1.9646374006749916 2316 92-93 -2.208335638538653 2316 94-95 -2.357605842051086 2316 96-97 -2.647499562889333 2316 98-99 -3.1102273412336956 2316 100-101 -2.8474006344380065 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 3963.0 3 863.0 4 774.0 5 738.0 6 855.0 7 1053.0 8 1310.0 9 1643.0 10 1957.0 11 2861.0 12 4426.0 13 6997.0 14 6359.0 15 7001.0 16 9364.0 17 13340.0 18 11309.0 19 7127.0 20 6910.0 21 7252.0 22 8007.0 23 9085.0 24 10043.0 25 11485.0 26 13285.0 27 15686.0 28 18632.0 29 22281.0 30 27053.0 31 33089.0 32 41247.0 33 53687.0 34 74495.0 35 116859.0 36 154553.0 37 226978.0 38 222076.0 39 36640.0 40 8.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.97827858913559 21.303082560896744 16.00787817956456 18.710760670403104 2 14.169257821984479 24.49526030714117 38.79390086425987 22.541581006614482 3 21.19516904478229 27.619946259236166 32.30125748009928 18.883627215882264 4 11.155420666290231 18.50909830335296 42.26215683902019 28.073324191336614 5 10.556855758686034 40.429249108182034 35.7647365302924 13.24915860283953 6 25.621615810563505 40.089799915508124 17.78184466092805 16.506739613000327 7 23.344506717888976 36.09795511104653 23.86389692153798 16.69364124952651 8 23.468296743122696 31.619518922510053 21.59506945546812 23.31711487889913 9 23.369525503763263 20.23519451296795 25.351569685256624 31.04371029801216 10-11 23.48478294648519 26.996400989412944 28.36331108974009 21.155504974361776 12-13 27.302043241722362 26.128507326987965 27.87518398834233 18.69426544294734 14-15 22.444336287310886 27.83326313384781 30.4191098257303 19.303290753111 16-17 18.10637702801787 30.924138672454067 30.626691852149236 20.342792447378834 18-19 17.881486409188383 31.207713765185247 32.203222552322586 18.707577273303784 20-21 20.419714520544954 27.525328143459575 31.18724661881937 20.8677107171761 22-23 19.0736262522268 27.758532406363113 32.64045795722949 20.5273833841806 24-25 21.501952374250923 28.226185550120043 31.370862888505673 18.900999187123364 26-27 19.98463399030236 26.444785071194865 35.07776889571822 18.49281204278455 28-29 18.102045428387324 30.635303487402055 32.607935895852606 18.654715188358022 30-31 19.28215540712527 27.873423931625368 31.841768573643947 21.002652087605416 32-33 19.49087371969789 28.400520625742075 32.86775492958574 19.240850724974287 34-35 21.29965206085735 27.6184694045963 32.026568673351505 19.05530986119485 36-37 20.091236573584162 26.842287510770866 34.22809733869427 18.838378576950703 38-39 19.246051072000974 29.021885289760935 33.06684578677707 18.665217851461023 40-41 18.903590168205607 29.503604164962745 32.530445054718044 19.0623606121136 42-43 18.46512618419582 29.510043920407703 30.339197118922463 21.685632776474012 44-45 19.668474407858973 27.398392053642635 31.219658057297483 21.713475481200913 46-47 21.632616052884057 29.233834832206984 31.1548672099864 17.97868190492256 48-49 20.30123012706195 28.682156155781225 31.294636605517432 19.72197711163939 50-51 17.366413823587965 29.54746375222278 33.49920536639189 19.58691705779736 52-53 19.484116088220567 31.00398537108575 29.54438380696784 19.96751473372584 54-55 18.421136979761496 28.646709324354187 31.11300800801645 21.819145687867863 56-57 20.6254427390791 28.15491651205937 30.185655253837073 21.033985495024456 58-59 22.356699256783447 27.895473288850237 30.169769058599393 19.578058395766927 60-61 19.95883689795065 29.25677591941674 32.507009063214944 18.277378119417666 62-63 18.65154552052281 29.374104144623896 32.25087788028441 19.723472454568878 64-65 18.36990957908319 31.33590714699378 30.62479727315789 19.669386000765144 66-67 19.755197703839507 28.973038506415765 29.09802975323193 22.173734036512798 68-69 18.556669715512324 28.996125378433746 30.974106187581636 21.47309871847229 70-71 21.338156278266034 28.903159720815953 29.548553454025082 20.21013054689293 72-73 20.094397604668163 27.255040999978487 32.55038468985422 20.10017670549913 74-75 18.35912881711993 31.800453055594225 29.844003762808104 19.99641436447774 76-77 19.202509512310122 28.824471625017186 29.89100592205093 22.082012940621762 78-79 19.536066129530084 28.72703859186334 30.987549270783965 20.749346007822606 80-81 20.986434043967176 28.739282405582067 29.737700673553867 20.536582876896887 82-83 19.843138114874044 27.999736848541996 31.919217562998202 20.23790747358575 84-85 19.13613385699592 29.231623698416907 30.878310020002136 20.753932424585038 86-87 19.001675499624035 30.036693990004355 30.258603857149424 20.703026653222185 88-89 18.974429914330575 29.434171864344304 29.032584578154108 22.558813643171014 90-91 19.844937174711625 27.398566126521175 29.996486389928045 22.760010308839153 92-93 21.21453329671873 29.202168697975523 30.005327677474302 19.57797032783144 94-95 19.94929254237574 28.145887296136614 30.549344852897747 21.355475308589895 96-97 17.862953487538256 28.495837648244894 32.255768975241025 21.38543988897583 98-99 19.59867611798512 29.52361507868881 29.076387283578363 21.80132151974771 100-101 19.095391129780523 27.159480068154597 30.77428850987736 22.97084029218753 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 9965.0 1 5992.0 2 2200.0 3 2174.5 4 1698.5 5 1747.0 6 2920.5 7 5543.5 8 8403.0 9 8838.0 10 6406.5 11 4161.5 12 3716.0 13 3659.5 14 3634.0 15 3696.5 16 3739.5 17 3653.0 18 3425.5 19 3387.5 20 3623.5 21 4056.0 22 4538.0 23 5234.5 24 5894.0 25 6662.5 26 8057.0 27 9531.0 28 10441.0 29 11882.0 30 14689.0 31 17545.0 32 21223.0 33 25745.0 34 29503.0 35 32967.0 36 39367.5 37 44915.5 38 44874.5 39 43637.5 40 40914.5 41 37210.5 42 33997.5 43 33744.0 44 34725.0 45 34337.0 46 36653.5 47 57311.0 48 100309.5 49 95914.0 50 51002.5 51 28680.0 52 20523.5 53 17609.5 54 15683.0 55 13491.5 56 11670.0 57 10299.5 58 9429.5 59 8558.5 60 7305.5 61 6186.0 62 5074.0 63 3636.5 64 2369.0 65 1664.5 66 1139.0 67 856.0 68 685.0 69 485.5 70 356.0 71 267.0 72 205.5 73 172.0 74 121.0 75 91.5 76 79.5 77 56.0 78 34.5 79 21.5 80 11.0 81 7.5 82 5.5 83 5.5 84 6.0 85 4.5 86 4.0 87 2.0 88 1.0 89 1.5 90 2.0 91 1.5 92 0.5 93 0.5 94 0.0 95 0.0 96 1.0 97 1.0 98 0.5 99 0.5 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.31117501936974257 2 0.08595716747629253 3 0.09510690502992132 4 0.09896826216264541 5 0.1096289655508184 6 0.0540589998581371 7 0.05573785078540844 8 0.05640939115631697 9 0.05892766754722398 10-11 0.057584586805406915 12-13 0.05527616678040882 14-15 0.053429430760410346 16-17 0.05523419550722703 18-19 0.06245325449449379 20-21 0.06442590433403761 22-23 0.0807107583285696 24-25 0.09011232352128909 26-27 0.08406846018311226 28-29 0.08209581034356844 30-31 0.07621983209811876 32-33 0.06719600836403532 34-35 0.31285387029701395 36-37 0.4869926827282335 38-39 0.4612223209946184 40-41 0.4450633808196318 42-43 0.4720509094755186 44-45 0.48195612994641945 46-47 0.4766677495255147 48-49 0.4940018853495913 50-51 0.4901405282168672 52-53 0.48942701657277693 54-55 0.48090684811687484 56-57 0.46092852208234597 58-59 0.4446856393609958 60-61 0.4022107109010309 62-63 0.471966966929155 64-65 0.4944635693545909 66-67 0.50432681855231 68-69 0.5156590623113916 70-71 0.5139802113841203 72-73 0.5024381112591297 74-75 0.504158933459583 76-77 0.4803192502923299 78-79 0.4614321773605274 80-81 0.4671822417864317 82-83 0.4752826975105159 84-85 0.4757443815155155 86-87 0.4763319793400605 88-89 0.4822919001318738 90-91 0.49530299481822665 92-93 0.5012629156100399 94-95 0.508775773509579 96-97 0.5005913752391313 98-99 0.5027319101714023 100-101 0.48275358413687336 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1191291.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 34.923366974518544 #Duplication Level Percentage of deduplicated Percentage of total 1 74.96848613294483 26.18151952744937 2 12.667508046559897 8.847840643253555 3 4.562423027265925 4.780055210226052 4 2.212168899671315 3.090255451713529 5 1.2533543321320135 2.188567664507445 6 0.7758647283492188 1.6257485178434945 7 0.542799959829675 1.3269481533619965 8 0.3879869915779112 1.0839849670571864 9 0.294698299848069 0.9262671185124736 >10 1.9122395342661958 13.224606500837401 >50 0.24674327535648427 6.075923114403232 >100 0.15983914622796222 10.000908092434258 >500 0.005777318534735054 1.272202401664914 >1k 0.006018040140349015 4.889316029764735 >5k 0.002888659267367527 7.361422275939219 >10k+ 0.001203608028069803 7.1244343310311775 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 30493 2.559660066264246 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 18464 1.5499151760568997 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 13029 1.0936874365709135 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 12694 1.0655666835391184 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 10066 0.8449656716956646 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 9676 0.8122280786138735 No Hit GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT 9035 0.7584209063948271 No Hit ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC 7996 0.6712046007230811 No Hit TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC 7854 0.6592847591394546 No Hit TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT 7405 0.621594555822213 No Hit GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA 7287 0.6116893353513122 No Hit GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG 7024 0.589612445657694 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6801 0.5708932578186187 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 6568 0.5513346445159075 No Hit ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC 6564 0.5509988743304532 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6100 0.5120495328177582 No Hit GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA 5255 0.4411180811405442 No Hit CCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCAC 4676 0.3925153467960389 No Hit GATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATA 4592 0.3854641729014993 No Hit TGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGAT 4239 0.3558324540351602 No Hit AAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGC 4175 0.35046013106789187 No Hit GTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTG 3808 0.31965321655246287 No Hit TACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACC 3377 0.2834739790697655 No Hit ATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATAC 2942 0.24695897140161385 No Hit GCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAG 2819 0.23663403819889514 No Hit CAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGT 2532 0.21254252739255144 No Hit ACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTG 2354 0.19760075413983655 No Hit GCTTTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC 2231 0.18727582093711778 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2144 0.17997281940348747 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1983 0.16645806943895322 No Hit CTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCT 1781 0.1495016750735127 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1630 0.13682635057261408 No Hit GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 1614 0.135483269830797 No Hit CACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACT 1415 0.1187787031044472 No Hit AACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACG 1392 0.11684802453808514 No Hit AGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAG 1371 0.11508523106445025 No Hit GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA 1265 0.10618732114991214 No Hit AGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTA 1234 0.10358510221264158 No Hit ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1193 0.10014345781173534 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 8.394254636356691E-5 0.0 12-13 0.0 0.0 0.0 8.394254636356691E-5 0.0 14-15 0.0 0.0 0.0 8.394254636356691E-5 0.0 16-17 0.0 0.0 0.0 4.616840049996181E-4 0.0 18-19 0.0 0.0 0.0 5.875978245449684E-4 0.0 20-21 0.0 0.0 0.0 9.653392831810196E-4 0.0 22-23 0.0 0.0 0.0 0.0022664487518163066 0.0 24-25 0.0 0.0 0.0 0.0037354433131787275 0.0 26-27 0.0 0.0 0.0 0.0052464091477229325 0.0 28-29 0.0 0.0 0.0 0.014228261608624594 0.0 30-31 0.0 0.0 0.0 0.036850777853605875 0.0 32-33 0.0 0.0 0.0 0.07256833133130361 0.0 34-35 0.0 0.0 0.0 0.12507439408171472 0.0 36-37 0.0 0.0 0.0 0.1941591097389303 0.0 38-39 0.0 0.0 0.0 0.29505805046793776 0.0 40-41 0.0 0.0 0.0 0.40372167673557513 0.0 42-43 0.0 0.0 0.0 0.517337913238663 0.0 44-45 0.0 0.0 0.0 0.6686863243321741 0.0 46-47 0.0 0.0 0.0 0.8533179550588396 0.0 48-49 0.0 0.0 0.0 1.0213289616055188 0.0 50-51 0.0 0.0 0.0 1.2016375511944606 0.0 52-53 0.0 0.0 0.0 1.3799734909438583 0.0 54-55 0.0 0.0 0.0 1.579672808742784 0.0 56-57 0.0 0.0 0.0 1.8561375851911919 0.0 58-59 0.0 0.0 0.0 2.1224872848027894 0.0 60-61 0.0 0.0 0.0 2.418342789461181 0.0 62-63 0.0 0.0 0.0 2.7239776007709287 0.0 64-65 0.0 0.0 0.0 3.033809539398854 0.0 66-67 0.0 0.0 0.0 3.403072800852185 0.0 68-69 0.0 0.0 0.0 3.825765493065926 0.0 70-71 0.0 0.0 0.0 4.281447606000549 0.0 72-73 0.0 0.0 0.0 4.719124042740187 0.0 74-75 0.0 0.0 0.0 5.223073119833861 0.0 76-77 0.0 0.0 0.0 5.696886822783014 0.0 78-79 0.0 0.0 0.0 6.35478652990747 0.0 80-81 0.0 0.0 0.0 7.038792368950995 0.0 82-83 0.0 0.0 0.0 7.654846716713212 0.0 84-85 0.0 0.0 0.0 8.331423640403562 0.0 86-87 0.0 0.0 0.0 9.031840247261165 0.0 88-89 0.0 0.0 0.0 9.791226492939172 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CTTTGCG 810 0.0 39.175316 2 GTAAGCG 300 0.0 35.675335 94-95 TTTGCGT 910 0.0 34.349884 3 TTGCGTT 975 0.0 32.54565 4 GCTTTGC 965 0.0 31.971203 1 GTACACG 60 0.003965312 31.643291 1 AATCGAC 30 0.004011282 31.570156 18-19 GAGTACT 6715 0.0 23.619764 12-13 GTACATG 6275 0.0 22.23855 1 ATGGGTA 410 0.0 21.947876 5 GTACTTT 7395 0.0 21.448282 14-15 ACATGGG 6130 0.0 21.401392 3 TACATGG 6600 0.0 20.738466 2 AGTACTT 7245 0.0 20.356186 12-13 GGTACTC 285 2.046363E-9 19.934858 8 GTATAGG 600 0.0 19.777058 1 CATGGGG 1315 0.0 19.448708 4 ACTTTTT 8495 0.0 19.146338 16-17 GTATGGT 255 2.6958514E-7 18.613699 1 TACTCCC 285 4.4181434E-8 18.279764 5 >>END_MODULE