##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938882_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1197980 Sequences flagged as poor quality 0 Sequence length 101 %GC 40 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.00325965375048 33.0 31.0 34.0 30.0 34.0 2 32.05041236080736 33.0 31.0 34.0 30.0 34.0 3 32.08374847660228 34.0 31.0 34.0 30.0 34.0 4 35.609394146813806 37.0 35.0 37.0 33.0 37.0 5 35.66537087430508 37.0 35.0 37.0 33.0 37.0 6 35.79394647656889 37.0 35.0 37.0 35.0 37.0 7 35.73531444598407 37.0 35.0 37.0 35.0 37.0 8 35.73239536553198 37.0 35.0 37.0 35.0 37.0 9 37.34261673817593 39.0 37.0 39.0 34.0 39.0 10-11 37.24760805689577 39.0 37.0 39.0 34.0 39.0 12-13 37.16299896492429 39.0 37.0 39.0 34.0 39.0 14-15 38.29116387585769 40.0 38.0 41.0 33.5 41.0 16-17 38.27450082639109 40.0 38.0 41.0 33.5 41.0 18-19 38.30144660177966 40.0 38.0 41.0 34.0 41.0 20-21 38.188753985876225 40.0 38.0 41.0 34.0 41.0 22-23 38.2735016444348 40.0 38.0 41.0 34.0 41.0 24-25 38.18206063540293 40.0 38.0 41.0 34.0 41.0 26-27 38.062789862935944 40.0 38.0 41.0 33.5 41.0 28-29 37.97312434264345 40.0 38.0 41.0 33.0 41.0 30-31 37.80294287049867 40.0 37.0 41.0 33.0 41.0 32-33 37.54063089534049 40.0 36.5 41.0 33.0 41.0 34-35 37.18120335898763 40.0 36.0 41.0 32.0 41.0 36-37 36.88270338402978 39.5 35.5 41.0 31.0 41.0 38-39 36.68281732583182 39.0 35.0 41.0 31.0 41.0 40-41 36.63231105694586 39.0 35.0 41.0 31.0 41.0 42-43 36.40379680796006 39.0 35.0 41.0 30.0 41.0 44-45 36.24147690278636 39.0 35.0 41.0 30.0 41.0 46-47 35.929726706622816 39.0 35.0 41.0 28.5 41.0 48-49 35.66388963087864 39.0 35.0 41.0 27.0 41.0 50-51 34.453836040668456 38.0 34.0 40.0 24.5 40.5 52-53 34.69544691898028 38.5 34.5 39.5 25.5 40.5 54-55 35.256841099183625 39.0 35.0 40.5 26.5 41.0 56-57 35.13566712299037 39.0 35.0 41.0 26.0 41.0 58-59 34.979073941134246 39.0 35.0 41.0 26.0 41.0 60-61 34.64114843319588 38.0 35.0 40.0 25.0 41.0 62-63 34.36755079383629 38.0 34.0 40.0 23.5 41.0 64-65 33.996055025960366 37.5 34.0 40.0 22.5 41.0 66-67 33.53269962770664 37.0 33.5 39.5 22.0 41.0 68-69 33.11659793986544 36.0 33.0 39.0 20.5 41.0 70-71 32.58986043172674 36.0 32.5 39.0 19.0 40.0 72-73 32.130368203141956 35.0 32.0 38.0 16.5 40.0 74-75 31.67713567839196 35.0 32.0 37.0 12.5 39.5 76-77 31.276362293193543 35.0 31.5 36.5 7.5 39.0 78-79 30.79835347835523 35.0 31.0 36.0 4.5 39.0 80-81 30.292890115026964 35.0 31.0 36.0 2.0 37.5 82-83 29.880558940883823 34.0 30.5 35.0 2.0 37.0 84-85 29.477541778660743 34.0 30.0 35.0 2.0 37.0 86-87 29.191982754303076 34.0 30.0 35.0 2.0 36.0 88-89 28.71532454631964 34.0 29.0 35.0 2.0 36.0 90-91 28.24204285547338 34.0 29.0 35.0 2.0 35.5 92-93 27.80497838027346 34.0 28.0 35.0 2.0 35.0 94-95 27.292468154727125 34.0 26.5 35.0 2.0 35.0 96-97 26.691328736706787 34.0 25.0 35.0 2.0 35.0 98-99 26.084066094592565 33.5 24.5 35.0 2.0 35.0 100-101 23.47381759294813 30.0 10.0 34.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.15333180634497978 1101 2 0.1564331132673118 1101 3 0.20101389185977325 1101 4 0.10846131011913229 1101 5 -0.059866746164431106 1101 6 0.05444253772648011 1101 7 0.13811398398473784 1101 8 0.13824035269959012 1101 9 0.05775187268507409 1101 10-11 0.18151313834921723 1101 12-13 0.3200856133901695 1101 14-15 0.17673745057212642 1101 16-17 0.2837823130685493 1101 18-19 0.2721406584329955 1101 20-21 0.1542568369776589 1101 22-23 0.22369616212539967 1101 24-25 0.294459543409495 1101 26-27 0.2844923749737944 1101 28-29 0.23259799004895143 1101 30-31 0.2657868160801016 1101 32-33 0.10384492973525283 1101 34-35 0.15050311040570818 1101 36-37 0.17623160869072763 1101 38-39 0.1825577477942275 1101 40-41 -0.05692206142986578 1101 42-43 -0.07779802386290413 1101 44-45 0.10130197955715659 1101 46-47 0.24225135971264677 1101 48-49 0.44061481951877823 1101 50-51 0.21988691784526537 1101 52-53 0.2287650244885242 1101 54-55 0.30656023635994956 1101 56-57 0.3836370885734013 1101 58-59 0.3657884103671023 1101 60-61 0.33370205396909114 1101 62-63 0.40709596907602474 1101 64-65 0.39768565849828974 1101 66-67 0.4501756903028209 1101 68-69 0.3667685292102263 1101 70-71 0.419678283997861 1101 72-73 0.3593704229664709 1101 74-75 0.1745506347130501 1101 76-77 0.42279365587998186 1101 78-79 0.16729269245071876 1101 80-81 0.04658549720466354 1101 82-83 -0.038935231054257 1101 84-85 0.04855723866099737 1101 86-87 0.07252575027743546 1101 88-89 -0.04392450843828399 1101 90-91 0.07191932488179731 1101 92-93 0.22764415347284128 1101 94-95 0.06089301613383924 1101 96-97 0.16600101526899635 1101 98-99 0.05402760998657996 1101 100-101 0.41441552548485916 1102 1 0.3423122489193915 1102 2 0.22201087546148557 1102 3 0.2648969368963918 1102 4 0.2859975420031873 1102 5 0.29180654353480406 1102 6 0.3739649002011589 1102 7 0.3552520547343079 1102 8 0.24255311567294058 1102 9 0.4303851125167739 1102 10-11 0.3343152655114423 1102 12-13 0.278638091183538 1102 14-15 0.4213172775941061 1102 16-17 0.4226663320805457 1102 18-19 0.567323376213885 1102 20-21 0.5993928039404182 1102 22-23 0.48644062994525683 1102 24-25 0.4534561539751749 1102 26-27 0.5420942527983215 1102 28-29 0.5135898865738042 1102 30-31 0.43897424790181816 1102 32-33 0.4423792915865832 1102 34-35 0.38906863946913717 1102 36-37 0.40249582873124723 1102 38-39 0.40235477132547715 1102 40-41 0.24647613866363116 1102 42-43 0.25378215379020475 1102 44-45 0.6307939540000405 1102 46-47 0.5783702626267768 1102 48-49 0.2621922700408348 1102 50-51 0.32985360788733686 1102 52-53 0.3673081521182553 1102 54-55 0.5270049535174977 1102 56-57 0.49427095174933555 1102 58-59 0.4259216501988021 1102 60-61 0.31456343779331064 1102 62-63 0.23314396658264513 1102 64-65 -0.018133649589607614 1102 66-67 0.24607427998648035 1102 68-69 0.11938852297678437 1102 70-71 0.15142111553907966 1102 72-73 0.21149661121643248 1102 74-75 0.20157845144984776 1102 76-77 0.010918948072593082 1102 78-79 0.10378949781147995 1102 80-81 0.12418698543903872 1102 82-83 -0.16226486328115186 1102 84-85 -0.08447805820669885 1102 86-87 -0.2857559623608701 1102 88-89 -0.16615354848815045 1102 90-91 -0.005920787320164322 1102 92-93 -0.29478649948025293 1102 94-95 -0.23126550809866941 1102 96-97 -0.22418326087250406 1102 98-99 -0.07489264090947856 1102 100-101 6.707849519003162E-4 1103 1 -0.10743152841557091 1103 2 0.1465407060689472 1103 3 0.22159097462546384 1103 4 0.18043203916821682 1103 5 0.1655940183056117 1103 6 0.17594585276546582 1103 7 0.18147709428276926 1103 8 0.10201009048065401 1103 9 0.1700439529206932 1103 10-11 0.2849787650926956 1103 12-13 0.26388443074515067 1103 14-15 0.21697649342760172 1103 16-17 0.29981703015494787 1103 18-19 0.308250093643764 1103 20-21 0.31085431599105817 1103 22-23 0.30489556074748947 1103 24-25 0.37363164323693354 1103 26-27 0.29622678585698736 1103 28-29 0.20515322988651263 1103 30-31 0.30981123124494303 1103 32-33 0.3931210056632679 1103 34-35 0.24126771020070947 1103 36-37 0.36059010647853995 1103 38-39 0.2930333683098496 1103 40-41 0.3033958352395203 1103 42-43 0.24778129704065321 1103 44-45 -0.08040724416580503 1103 46-47 -0.02864441442356025 1103 48-49 -0.005450112428853515 1103 50-51 0.0932379130056944 1103 52-53 0.13625410951181038 1103 54-55 0.30650144469490925 1103 56-57 0.3347767548638032 1103 58-59 0.35481504745143155 1103 60-61 0.20760812353950087 1103 62-63 0.16364657694128937 1103 64-65 0.10227335457862807 1103 66-67 0.1097099143501552 1103 68-69 0.11154701843472736 1103 70-71 0.12074699483044782 1103 72-73 0.15970217014122312 1103 74-75 0.13229605139594014 1103 76-77 0.1941694943707617 1103 78-79 0.3751183901658699 1103 80-81 0.2576301399459595 1103 82-83 0.4865969754253143 1103 84-85 0.1609060832628657 1103 86-87 0.18611489628187172 1103 88-89 0.317214038783181 1103 90-91 0.21767399902189766 1103 92-93 0.185945860944976 1103 94-95 0.1943505012453528 1103 96-97 0.31970188976460534 1103 98-99 0.0580282814279407 1103 100-101 0.37445665532280614 1104 1 0.32714491633896614 1104 2 0.32342036655153095 1104 3 0.3440346972117325 1104 4 0.3843352883991855 1104 5 0.3530160390537276 1104 6 0.3526200707604943 1104 7 0.292751857016448 1104 8 0.32572036600273435 1104 9 0.3689391464356646 1104 10-11 0.35960140280040065 1104 12-13 0.45237396226515614 1104 14-15 0.5049649823269959 1104 16-17 0.39902520739807557 1104 18-19 0.597735942212104 1104 20-21 0.5697746683091296 1104 22-23 0.532460648483287 1104 24-25 0.537933087418601 1104 26-27 0.5034558328962078 1104 28-29 0.44262459633980455 1104 30-31 0.5257557807372208 1104 32-33 0.6313770043863727 1104 34-35 0.5390317057726293 1104 36-37 0.6070432604553133 1104 38-39 0.5886706390538095 1104 40-41 0.44669513096686586 1104 42-43 0.5530316958756387 1104 44-45 0.6449161499653329 1104 46-47 0.6440351324335225 1104 48-49 0.5849979181283445 1104 50-51 0.444067703114591 1104 52-53 0.46264335507168397 1104 54-55 0.5582131361898277 1104 56-57 0.6455195795873792 1104 58-59 0.5925206973568891 1104 60-61 0.5489196051670149 1104 62-63 0.4258306992396257 1104 64-65 0.6004970672246444 1104 66-67 0.6212526214974261 1104 68-69 0.587259370506267 1104 70-71 0.5993726794082761 1104 72-73 0.5543698313602405 1104 74-75 0.41022496987557133 1104 76-77 0.2516467712463921 1104 78-79 0.37013048361691503 1104 80-81 0.4405612669323311 1104 82-83 0.41076025163246754 1104 84-85 0.28277654229249904 1104 86-87 0.3729869632940499 1104 88-89 0.6133408157619265 1104 90-91 0.7066488489106035 1104 92-93 0.6435425315124164 1104 94-95 0.6535733281181813 1104 96-97 0.7981778383755369 1104 98-99 0.5857740795364457 1104 100-101 1.3350219698354984 1105 1 -0.12035954073949995 1105 2 -0.09204517902626108 1105 3 0.028303602381789972 1105 4 -0.11115481416911166 1105 5 -0.1134297575902039 1105 6 -0.04401360063972248 1105 7 -0.024579116559522163 1105 8 -0.02501209523082082 1105 9 0.011790329389071985 1105 10-11 -0.04341953570837376 1105 12-13 -0.08671666359362007 1105 14-15 -0.13002727471916842 1105 16-17 -0.07190331170362896 1105 18-19 -0.07598945582471117 1105 20-21 0.04864937943150238 1105 22-23 -0.15803132571350176 1105 24-25 -0.08346249676295514 1105 26-27 -0.1465063900625907 1105 28-29 0.12167437553356564 1105 30-31 0.050267989955436576 1105 32-33 -0.05440795813129995 1105 34-35 -0.0445520073086314 1105 36-37 0.12285871252823455 1105 38-39 0.20014812324830444 1105 40-41 0.12152743075812111 1105 42-43 -0.035905104320939074 1105 44-45 -0.0511091847652807 1105 46-47 -0.008194861235857331 1105 48-49 -0.16963082197096213 1105 50-51 -0.1502765341451351 1105 52-53 -0.11077730051116674 1105 54-55 0.014264696684001876 1105 56-57 -0.05667064676101319 1105 58-59 -0.14282491089940663 1105 60-61 -0.0569510312402528 1105 62-63 -0.06803349784706825 1105 64-65 -0.10200088699739496 1105 66-67 -0.10024217047624262 1105 68-69 -0.06455036793270352 1105 70-71 -0.16968099614090448 1105 72-73 0.1623964766135444 1105 74-75 0.12146369704571924 1105 76-77 0.1302266913136947 1105 78-79 0.1031405751008947 1105 80-81 0.12294859221830023 1105 82-83 0.08561972639763127 1105 84-85 -0.08319342438562671 1105 86-87 0.052882994140325934 1105 88-89 -0.07673549615778441 1105 90-91 -0.04802926543709063 1105 92-93 0.024543703481011647 1105 94-95 -0.2271162885156599 1105 96-97 -0.446881100698171 1105 98-99 -0.5180940292155007 1105 100-101 0.08496934224517005 1106 1 0.023093385345688944 1106 2 0.042461034621325666 1106 3 0.15524896837745672 1106 4 0.1009742319798761 1106 5 0.08601726113261776 1106 6 -0.06243695958779938 1106 7 0.06155829329861007 1106 8 0.07242754232156301 1106 9 0.05238676677648613 1106 10-11 0.07028695511215943 1106 12-13 0.04136931157604806 1106 14-15 -0.06204143358718994 1106 16-17 0.12263594132434719 1106 18-19 0.16629541671817805 1106 20-21 0.2568842819964132 1106 22-23 0.024461972402086474 1106 24-25 0.032484652358519384 1106 26-27 0.1699343786223224 1106 28-29 0.06807863259481195 1106 30-31 0.016594432376848545 1106 32-33 0.0887060830746762 1106 34-35 0.01658449279143781 1106 36-37 -0.04124837509360191 1106 38-39 -0.1139356139165173 1106 40-41 -0.06014935949412603 1106 42-43 -0.3792447655270408 1106 44-45 -0.5208919497019906 1106 46-47 -0.43648204456423656 1106 48-49 -0.4941741234223329 1106 50-51 -0.3523076448384259 1106 52-53 -0.5303721245942725 1106 54-55 -0.8156241915309934 1106 56-57 -0.6642434728941211 1106 58-59 -0.5954333388981183 1106 60-61 -0.6505890172784987 1106 62-63 -0.8381850180527906 1106 64-65 -0.9566393643049977 1106 66-67 -0.979390667252666 1106 68-69 -0.7484007218285882 1106 70-71 -0.8397185691489923 1106 72-73 -0.7394849788780604 1106 74-75 -0.7253239472267481 1106 76-77 -0.7239201375729429 1106 78-79 -0.6365230570172038 1106 80-81 -0.5134283114504505 1106 82-83 -0.3224022169668963 1106 84-85 -0.46329413460342295 1106 86-87 -0.33362923604059347 1106 88-89 -0.24455856640929952 1106 90-91 -0.20203045722015744 1106 92-93 -0.25948465974292745 1106 94-95 -0.18007118271079747 1106 96-97 -0.4195049642587243 1106 98-99 -0.4562433690344001 1106 100-101 0.02648099624598288 1107 1 -0.01047008508218994 1107 2 -0.017998844482626453 1107 3 0.0633235209435341 1107 4 -0.08829624330754626 1107 5 -0.030804476451834262 1107 6 -0.04042972952971979 1107 7 -0.10561605777404282 1107 8 0.010231264023587983 1107 9 0.0360895047165144 1107 10-11 0.042817179125442806 1107 12-13 0.013206161167133246 1107 14-15 -0.11166067028269566 1107 16-17 -0.057631037459579204 1107 18-19 0.009161660643748348 1107 20-21 0.005291595022363538 1107 22-23 -0.09431691819884236 1107 24-25 0.038206472910850664 1107 26-27 -0.030849371525832225 1107 28-29 -0.0063420738174215785 1107 30-31 0.1897320997941776 1107 32-33 0.14121289829335382 1107 34-35 -0.018976133023429043 1107 36-37 -0.06255185974454491 1107 38-39 -0.07999138018415408 1107 40-41 -0.14371431149128 1107 42-43 -0.08900534393168158 1107 44-45 0.2563437383387779 1107 46-47 0.29170481095312795 1107 48-49 0.31285444123334827 1107 50-51 0.1525800522102898 1107 52-53 0.20621374716107965 1107 54-55 0.2563652596957411 1107 56-57 0.4074733695854391 1107 58-59 0.1824735000256652 1107 60-61 0.1776808061184454 1107 62-63 0.12955973719196123 1107 64-65 0.30318492157861243 1107 66-67 0.17750159302942592 1107 68-69 0.17441100034753987 1107 70-71 0.2396994704385378 1107 72-73 0.1528686420772516 1107 74-75 0.35169691326868957 1107 76-77 0.20177916312635702 1107 78-79 0.2472300542782797 1107 80-81 0.1458685357701235 1107 82-83 0.06217168370870141 1107 84-85 0.03926898024782943 1107 86-87 0.1740778873444917 1107 88-89 0.14696685731951575 1107 90-91 0.3062352177828025 1107 92-93 0.07436179041707902 1107 94-95 -0.16479324031421072 1107 96-97 -0.009554744986470354 1107 98-99 0.07955727100353016 1107 100-101 -0.0016984514249891447 1108 1 0.12821356456766608 1108 2 0.027905231143364517 1108 3 0.05894631893109903 1108 4 0.09901436549905185 1108 5 0.025846144823773898 1108 6 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68-69 -2.188598476091016 2316 70-71 -1.905382564402693 2316 72-73 -1.915147199133468 2316 74-75 -1.900005663285377 2316 76-77 -2.140362700573224 2316 78-79 -1.7648140983819616 2316 80-81 -2.002354632000557 2316 82-83 -2.136372669323997 2316 84-85 -2.528291897619262 2316 86-87 -2.365603520448815 2316 88-89 -2.501164964183989 2316 90-91 -2.5801689216764245 2316 92-93 -2.6401056903118025 2316 94-95 -2.8143583142162854 2316 96-97 -2.9054435736326596 2316 98-99 -2.934280804477954 2316 100-101 -3.809093373751196 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 4489.0 3 853.0 4 711.0 5 770.0 6 924.0 7 1089.0 8 1348.0 9 1659.0 10 1946.0 11 2738.0 12 4260.0 13 6960.0 14 6881.0 15 6708.0 16 9218.0 17 13078.0 18 11015.0 19 7059.0 20 6737.0 21 6968.0 22 7780.0 23 8649.0 24 9506.0 25 11069.0 26 12908.0 27 14995.0 28 17907.0 29 21467.0 30 25800.0 31 32084.0 32 40432.0 33 52237.0 34 72128.0 35 114106.0 36 153908.0 37 229984.0 38 235680.0 39 41919.0 40 10.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.062587104404756 21.259961814265996 15.98594443565553 18.69150664567372 2 14.186034271449731 24.47523878984739 38.72693870287801 22.611788235824868 3 21.369384392764168 27.58828404621148 32.226726330317845 18.815605230706506 4 11.190418096922013 18.459569034957838 42.21921594711036 28.1307969210098 5 10.567880808536595 40.47120068854294 35.70454488481111 13.256373618109349 6 25.691574458131267 40.039180795562295 17.778891659670094 16.490353086636343 7 23.247804978306693 36.13512427386117 23.86706469058319 16.75000605724894 8 23.512874731506646 31.62979121221564 21.583565658582557 23.273768397695154 9 23.362804057836367 20.173549203187584 25.412609637946215 31.051037101029834 10-11 23.442361224823973 26.970513930150414 28.332993587151577 21.254131257874036 12-13 27.37110118283006 26.19470431337164 27.73374104731529 18.700453456483007 14-15 22.492693149022998 27.867176572623165 30.392113447112436 19.248016831241404 16-17 18.125538610121673 30.906310348875245 30.637250704187696 20.33090033681539 18-19 17.84113620716162 31.217873304679717 32.20118413400801 18.73980635415065 20-21 20.439680999198277 27.62095906624292 31.103915066809435 20.83544486774936 22-23 19.076113252736178 27.728134602319304 32.70130556026778 20.494446584676734 24-25 21.540732927757798 28.10569090062675 31.398575331657874 18.955000839957574 26-27 20.036884161750383 26.401972170135195 35.084335013166054 18.476808654948375 28-29 18.10399618571171 30.56645607572081 32.689804509367946 18.639743229199528 30-31 19.250627694259308 27.865530319648784 31.878260949499076 21.00558103659283 32-33 19.452342225317736 28.368002560343342 32.924561371989725 19.2550938423492 34-35 21.33686808405814 27.612488987070204 32.00542780859559 19.045215120276065 36-37 20.07325988874169 26.8541233244508 34.20634840676953 18.866268380037983 38-39 19.24772084632874 28.988954378312858 33.0978736013545 18.665451174003902 40-41 18.907867814044852 29.451848756524484 32.60264960954823 19.037633819882434 42-43 18.420000159472163 29.53688863932894 30.36992086403697 21.673190337161923 44-45 19.641594874266055 27.355633051984356 31.26793410826031 21.734837965489277 46-47 21.637864279373716 29.250340487237764 31.202019629692586 17.90977560369594 48-49 20.30043150874415 28.641786452077927 31.342504765738905 19.715277273439018 50-51 17.316489596144883 29.47286014716932 33.59578388861138 19.614866368074416 52-53 19.421624984363365 31.01590697104305 29.588418563142206 19.97404948145138 54-55 18.419533849412936 28.602710907977464 31.08486267701848 21.892892565591122 56-57 20.628588476843035 28.15379986731841 30.14910104236034 21.068510613478217 58-59 22.371557456185805 27.868634411990417 30.135446479109035 19.624361652714743 60-61 20.010341586892604 29.208440848513366 32.55121412997941 18.230003434614623 62-63 18.564129036590305 29.391140852843478 32.24781606011579 19.79691405045043 64-65 18.33947558674684 31.338087597014436 30.629986120102675 19.69245069613605 66-67 19.728960307696184 28.949390528097013 29.11230826723548 22.209340896971323 68-69 18.513265150589472 28.918040881933166 31.052205273812206 21.516488693665153 70-71 21.31827260714959 28.810524817489746 29.666319148051745 20.204883427308918 72-73 20.043395532476953 27.22431493333647 32.646699235707935 20.085590298478635 74-75 18.42513939578158 31.801172767641788 29.812539385789506 19.961148450787128 76-77 19.182380433076187 28.787107953386343 29.910641202263687 22.11987041127378 78-79 19.528416620993376 28.72729729661676 30.984830871731774 20.75945521065809 80-81 20.960958717860056 28.768375873263125 29.664425249347886 20.606240159528934 82-83 19.862471533605245 27.957420233237666 31.93772102639348 20.242387206763606 84-85 19.097380792478173 29.208655137676292 30.967008059100067 20.726956010745468 86-87 19.014978293058814 30.109944901638848 30.23207792338608 20.642998881916256 88-89 18.926248813656933 29.464459239539924 29.04541411505838 22.563877831744755 90-91 19.827093644245153 27.42664410283365 30.04206998958431 22.704192263336893 92-93 21.201002773960624 29.226679228631543 30.050302440220456 19.52201555718738 94-95 20.0024267197802 28.125808206847214 30.569573804189744 21.302191269182842 96-97 17.82971739918074 28.487042546929754 32.37329186522922 21.309948188660282 98-99 19.626456628117147 29.573324208524276 29.05619104998982 21.744028113368756 100-101 19.051103009849538 27.326394791686344 30.659287382809797 22.96321481565432 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 10214.0 1 6215.5 2 2298.5 3 2203.0 4 1703.5 5 1729.5 6 2943.5 7 5687.0 8 8513.0 9 8820.5 10 6401.5 11 4259.0 12 3833.0 13 3785.0 14 3754.5 15 3704.5 16 3859.0 17 3853.0 18 3614.5 19 3528.0 20 3689.0 21 4161.0 22 4592.0 23 5193.5 24 5872.0 25 6640.0 26 8012.5 27 9429.5 28 10494.5 29 12096.5 30 14719.5 31 17473.5 32 21159.0 33 25819.5 34 29532.0 35 33196.5 36 39773.5 37 45099.0 38 44893.0 39 43429.0 40 40846.5 41 37233.5 42 34300.0 43 33971.0 44 34600.0 45 34375.5 46 36797.5 47 57828.0 48 101227.5 49 96856.0 50 51627.5 51 28919.0 52 20585.0 53 17638.0 54 15670.5 55 13511.5 56 11719.0 57 10288.5 58 9551.5 59 8608.0 60 7256.5 61 6194.0 62 5057.0 63 3661.0 64 2323.5 65 1630.5 66 1185.5 67 880.5 68 696.0 69 487.0 70 334.0 71 230.0 72 178.0 73 159.5 74 117.5 75 92.0 76 83.0 77 57.5 78 33.5 79 20.0 80 14.0 81 10.0 82 9.0 83 6.5 84 5.0 85 3.0 86 2.5 87 4.0 88 3.0 89 0.5 90 0.5 91 1.0 92 1.0 93 1.0 94 0.5 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.27487938029015513 2 0.08405816457703802 3 0.08748059233042288 4 0.10217198951568475 5 0.1043423095544166 6 0.08055226297600962 7 0.08906659543565001 8 0.08539374613933455 9 0.09065259854087715 10-11 0.08414163842468154 12-13 0.0886909631212541 14-15 0.09666271557121153 16-17 0.08627022153959166 18-19 0.09649576787592447 20-21 0.09854087714319104 22-23 0.11093674351825573 24-25 0.12445950683650812 26-27 0.12784019766607121 28-29 0.11907544366350023 30-31 0.1117297450708693 32-33 0.10563615419289137 34-35 0.3753401559291474 36-37 0.574133124092222 38-39 0.55873219920199 40-41 0.5191655954189552 42-43 0.5467119651413211 44-45 0.5687907978430358 46-47 0.5569792484014758 48-49 0.5781816057029333 50-51 0.57054374864355 52-53 0.573214911768143 54-55 0.5639075777558891 56-57 0.5346917310806524 58-59 0.5282642448121003 60-61 0.4763435115778226 62-63 0.5519708175428638 64-65 0.5884071520392661 66-67 0.600594333795222 68-69 0.6018047045860532 70-71 0.5973805906609458 72-73 0.5904939982303544 74-75 0.5873637289437219 76-77 0.5698759578624017 78-79 0.5465032805222124 80-81 0.5402427419489474 82-83 0.5561862468488623 84-85 0.5659526870231557 86-87 0.5556019299153575 88-89 0.5692499040050752 90-91 0.5833569842568324 92-93 0.5912869997829681 94-95 0.5903270505350674 96-97 0.5863620427719995 98-99 0.5916208951735421 100-101 0.5682482178333529 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1197980.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 35.126992270096395 #Duplication Level Percentage of deduplicated Percentage of total 1 74.97320538164456 26.335832059053775 2 12.64315299245235 8.882318748710395 3 4.737748827057285 4.992685992770984 4 2.1773077893992996 3.0592909555139944 5 1.207493784573922 2.1207812418458802 6 0.7341572350204397 1.5473241311758994 7 0.5310739156640363 1.30585205312663 8 0.3894224965931529 1.0943392822103457 9 0.29720015561029617 0.9395772811914888 >10 1.8898530884484046 13.144529098095212 >50 0.24382988812256642 5.970751737219129 >100 0.15985445437268186 10.038935800683992 >500 0.0057090876512932405 1.258240693940163 >1k 0.005946966303430458 4.8085216478603146 >5k 0.0028545438256466203 7.3621870647487 >10k+ 0.0011893932606860918 7.138832211853092 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 30575 2.55221289170103 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 18542 1.5477720830063941 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 13385 1.117297450708693 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 12600 1.0517704803085193 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 10332 0.8624517938529858 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 9852 0.8223843469840899 No Hit GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT 9121 0.761364964356667 No Hit ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC 8120 0.6778076428654902 No Hit TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC 7842 0.654601913220588 No Hit GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA 7411 0.6186246848862251 No Hit TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT 7235 0.6039332877009633 No Hit GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG 7177 0.5990918045376383 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6990 0.5834821950282976 No Hit ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC 6634 0.5537655052671998 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 6550 0.546753702065143 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6004 0.5011769812517738 No Hit GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA 5171 0.4316432661647106 No Hit CCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCAC 4660 0.38898813001886506 No Hit GATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATA 4524 0.37763568673934456 No Hit TGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGAT 4204 0.35092405549341393 No Hit AAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGC 4166 0.34775204928295966 No Hit GTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTG 3744 0.3125260855773886 No Hit TACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACC 3327 0.27771749111003524 No Hit ATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATAC 2956 0.24674869363428437 No Hit GCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAG 2806 0.2342276164877544 No Hit CAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGT 2530 0.2111888345381392 No Hit ACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTG 2340 0.1953288034858679 No Hit GCTTTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC 2263 0.18890131721731582 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2125 0.1773819262425082 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2028 0.1692849630210855 No Hit CTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCT 1745 0.14566186413796559 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1584 0.1322225746673567 No Hit GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 1484 0.12387518990300339 No Hit AGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAG 1410 0.11769812517738193 No Hit CACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACT 1335 0.11143758660411693 No Hit AACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACG 1267 0.10576136496435666 No Hit GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA 1259 0.1050935741832084 No Hit GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1210 0.10100335564867527 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 1.6694769528706655E-4 0.0 9 0.0 0.0 0.0 1.6694769528706655E-4 0.0 10-11 0.0 0.0 0.0 2.5042154293059986E-4 0.0 12-13 0.0 0.0 0.0 4.173692382176664E-4 0.0 14-15 0.0 0.0 0.0 4.173692382176664E-4 0.0 16-17 0.0 0.0 0.0 5.84316933504733E-4 0.0 18-19 0.0 0.0 0.0 6.260538573264996E-4 0.0 20-21 0.0 0.0 0.0 0.001043423095544166 0.0 22-23 0.0 0.0 0.0 0.001502529257583599 0.0 24-25 0.0 0.0 0.0 0.002796373896058365 0.0 26-27 0.0 0.0 0.0 0.004507587772750798 0.0 28-29 0.0 0.0 0.0 0.013021920232391192 0.0 30-31 0.0 0.0 0.0 0.036102439105828145 0.0 32-33 0.0 0.0 0.0 0.07475083056478406 0.0 34-35 0.0 0.0 0.0 0.12917577922836776 0.0 36-37 0.0 0.0 0.0 0.2020484482211723 0.0 38-39 0.0 0.0 0.0 0.3120252424915274 0.0 40-41 0.0 0.0 0.0 0.4308920015359188 0.0 42-43 0.0 0.0 0.0 0.558899146897277 0.0 44-45 0.0 0.0 0.0 0.7108215496085077 0.0 46-47 0.0 0.0 0.0 0.8999315514449323 0.0 48-49 0.0 0.0 0.0 1.073974523781699 0.0 50-51 0.0 0.0 0.0 1.2622915240655104 0.0 52-53 0.0 0.0 0.0 1.4532796874739144 0.0 54-55 0.0 0.0 0.0 1.666137998964924 0.0 56-57 0.0 0.0 0.0 1.9406834838645053 0.0 58-59 0.0 0.0 0.0 2.2057129501327233 0.0 60-61 0.0 0.0 0.0 2.5125628140703515 0.0 62-63 0.0 0.0 0.0 2.8141538256064376 0.0 64-65 0.0 0.0 0.0 3.132105711280656 0.0 66-67 0.0 0.0 0.0 3.51257950883988 0.0 68-69 0.0 0.0 0.0 3.9519858428354393 0.0 70-71 0.0 0.0 0.0 4.40487320322543 0.0 72-73 0.0 0.0 0.0 4.844279537220989 0.0 74-75 0.0 0.0 0.0 5.354054324780046 0.0 76-77 0.0 0.0 0.0 5.826098933204227 0.0 78-79 0.0 0.0 0.0 6.486877911150437 0.0 80-81 0.0 4.173692382176664E-5 0.0 7.183550643583365 0.0 82-83 0.0 8.347384764353328E-5 0.0 7.803719594650996 0.0 84-85 0.0 8.347384764353328E-5 0.0 8.484156663717258 0.0 86-87 0.0 8.347384764353328E-5 0.0 9.178033022254127 0.0 88-89 0.0 8.347384764353328E-5 0.0 9.93647640194327 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position ATAGCGT 60 0.0040211068 31.553112 6 CTTTGCG 785 0.0 30.75435 2 TTTGCGT 895 0.0 26.974487 3 GCTTTGC 910 0.0 26.576502 1 TTGCGTT 940 0.0 26.186749 4 CATGGGG 1305 0.0 25.391806 4 GTAAGCG 220 0.0 24.865183 94-95 GAGTACT 6815 0.0 24.552364 12-13 ATAGGAC 135 1.3789002E-5 24.545414 3 GTACTTT 7370 0.0 22.735554 14-15 GTACATG 6105 0.0 22.448181 1 AGTACTT 7425 0.0 22.184649 12-13 CATTCCG 150 3.1166368E-5 22.089025 9 CGTATAG 370 0.0 21.787941 1 GGATATG 330 1.8189894E-12 21.554916 1 ACTATAC 375 0.0 21.459703 3 TACCTGG 465 0.0 21.378263 2 ACATGGG 6095 0.0 20.73689 3 TACATGG 6575 0.0 20.446957 2 ATGGGAT 1650 0.0 20.369505 5 >>END_MODULE