##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938882_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1197980 Sequences flagged as poor quality 0 Sequence length 101 %GC 40 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.06158199636054 33.0 31.0 34.0 30.0 34.0 2 32.2782216731498 34.0 31.0 34.0 30.0 34.0 3 32.352825589742736 34.0 31.0 34.0 30.0 34.0 4 35.93721598023339 37.0 35.0 37.0 35.0 37.0 5 35.868837543197714 37.0 35.0 37.0 35.0 37.0 6 35.94813352476669 37.0 35.0 37.0 35.0 37.0 7 35.898415666371726 37.0 35.0 37.0 35.0 37.0 8 35.88496886425483 37.0 35.0 37.0 35.0 37.0 9 37.51153858995976 39.0 37.0 39.0 35.0 39.0 10-11 37.424965358353234 39.0 37.0 39.0 34.0 39.0 12-13 37.37992495701097 39.0 37.0 39.0 34.0 39.0 14-15 38.4729753418254 40.0 38.0 41.0 34.0 41.0 16-17 38.49861475149835 40.0 38.0 41.0 33.5 41.0 18-19 38.67671663967679 40.0 38.0 41.0 34.0 41.0 20-21 38.686728492963155 40.0 38.0 41.0 34.0 41.0 22-23 38.616657623666505 40.0 38.0 41.0 34.0 41.0 24-25 38.51070885991419 40.0 38.0 41.0 34.0 41.0 26-27 38.301690345414784 40.0 38.0 41.0 34.0 41.0 28-29 38.135247666905954 40.0 37.0 41.0 33.0 41.0 30-31 37.9727382760981 40.0 37.0 41.0 33.0 41.0 32-33 37.70301799696155 40.0 36.0 41.0 33.0 41.0 34-35 37.56791724402744 40.0 36.0 41.0 33.0 41.0 36-37 37.42054750496669 40.0 36.0 41.0 32.5 41.0 38-39 37.267507387435515 40.0 36.0 41.0 32.0 41.0 40-41 37.18892677674084 40.0 36.0 41.0 32.0 41.0 42-43 36.984712182173325 40.0 35.0 41.0 31.0 41.0 44-45 36.68716422644785 39.5 35.0 41.0 31.0 41.0 46-47 36.303319754920786 39.0 35.0 41.0 30.0 41.0 48-49 35.92275497086763 39.0 35.0 41.0 28.5 41.0 50-51 35.64778043039116 39.0 35.0 40.5 28.0 41.0 52-53 35.52834980550594 39.0 35.0 40.0 27.5 41.0 54-55 35.33907243860499 39.0 35.0 40.0 27.0 41.0 56-57 35.02062972670662 38.0 34.5 40.0 26.5 41.0 58-59 34.73935082388687 38.0 34.0 40.0 26.0 41.0 60-61 34.2254127781766 38.0 34.0 40.0 24.0 41.0 62-63 33.99215429305998 37.0 33.5 40.0 24.5 41.0 64-65 33.77857601963305 37.0 33.0 39.5 23.5 41.0 66-67 33.27584517270739 36.0 33.0 39.0 22.5 40.5 68-69 32.81262792367151 36.0 32.0 39.0 22.0 40.0 70-71 32.25938329521361 35.0 31.5 38.0 20.5 40.0 72-73 31.697338853737122 35.0 31.0 37.0 18.5 39.5 74-75 31.257649543398053 35.0 31.0 37.0 16.5 39.0 76-77 29.283418754904087 32.5 28.5 35.0 11.0 37.0 78-79 30.27274745822134 34.0 30.0 36.0 9.5 38.0 80-81 30.214095811282327 34.0 30.0 35.5 7.5 37.0 82-83 29.846934005576053 34.0 30.0 35.0 4.5 37.0 84-85 29.39545735321124 34.0 29.0 35.0 2.0 36.0 86-87 28.90829396150186 34.0 29.0 35.0 2.0 36.0 88-89 28.29535008931702 34.0 28.5 35.0 2.0 35.5 90-91 27.77627673249971 33.0 27.0 35.0 2.0 35.0 92-93 27.420621796691094 33.0 26.0 35.0 2.0 35.0 94-95 26.976116462712234 33.0 26.0 35.0 2.0 35.0 96-97 26.27701589342059 32.5 25.0 34.0 2.0 35.0 98-99 25.41643600060101 32.0 21.5 34.0 2.0 35.0 100-101 21.02353837292776 27.5 2.0 32.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.14420305858030957 1101 2 0.15030664024470042 1101 3 0.16295765976344256 1101 4 0.08656676650958417 1101 5 0.1016165229461592 1101 6 0.11560888552580906 1101 7 0.09663308291633399 1101 8 0.16814380469030255 1101 9 0.1569202987612357 1101 10-11 0.20577160907222947 1101 12-13 0.037776902733682505 1101 14-15 0.2580397044430498 1101 16-17 0.25983523972222855 1101 18-19 0.29025074901861814 1101 20-21 0.34488882361560513 1101 22-23 0.32985645719291057 1101 24-25 0.2363919799294436 1101 26-27 0.323972610723402 1101 28-29 0.25069700400565864 1101 30-31 0.22658868088218753 1101 32-33 0.45151160306063076 1101 34-35 0.5348893650488975 1101 36-37 0.5696412173207577 1101 38-39 0.5001573317335115 1101 40-41 0.5098707018124387 1101 42-43 0.281517913641288 1101 44-45 0.5167473539745728 1101 46-47 0.6221100625279945 1101 48-49 0.41081746850995415 1101 50-51 0.5400348476868615 1101 52-53 0.7016139547327924 1101 54-55 0.5214785115919511 1101 56-57 0.27307958946823163 1101 58-59 0.4561744344738443 1101 60-61 0.6694173257339244 1101 62-63 0.36808132228222945 1101 64-65 0.21914692834882743 1101 66-67 0.45378867854924465 1101 68-69 0.49813182576318127 1101 70-71 0.5105021586051706 1101 72-73 0.42687910508394467 1101 74-75 0.35732163867194444 1101 76-77 0.11390301129642211 1101 78-79 0.45819641857697135 1101 80-81 0.7564892839655428 1101 82-83 0.6771015082965341 1101 84-85 0.4241184997985634 1101 86-87 0.5614728404033649 1101 88-89 0.8029210904886028 1101 90-91 0.8644202307320654 1101 92-93 0.6747309832641868 1101 94-95 0.6506993383012691 1101 96-97 0.49617317190654475 1101 98-99 0.42833164517297106 1101 100-101 0.8737997234409072 1102 1 0.1919862906201999 1102 2 0.13748912427150373 1102 3 0.19133930227552298 1102 4 0.17670818773445518 1102 5 0.18685884802017938 1102 6 0.18287162512375232 1102 7 0.10818443089825536 1102 8 0.18444815251638857 1102 9 0.22022869832488823 1102 10-11 0.2591649392888442 1102 12-13 0.2286565972950001 1102 14-15 0.24345929303584768 1102 16-17 0.2557835021501589 1102 18-19 0.1886553773496118 1102 20-21 0.24167665281928663 1102 22-23 0.2715104621796698 1102 24-25 0.18152794689219576 1102 26-27 0.3174859347065677 1102 28-29 0.17049013163539684 1102 30-31 0.21919620776390047 1102 32-33 0.31193606153032505 1102 34-35 0.36955704464995165 1102 36-37 0.3289719014410579 1102 38-39 0.34348852232101024 1102 40-41 0.17395633372765928 1102 42-43 0.2529414760536284 1102 44-45 0.35040696905949886 1102 46-47 0.3852002916069992 1102 48-49 0.4409084378102577 1102 50-51 0.48354427576853 1102 52-53 0.375453950057711 1102 54-55 0.38048583588679463 1102 56-57 0.2913757169421345 1102 58-59 0.2415346105685927 1102 60-61 0.2746602351931742 1102 62-63 0.41894718639318995 1102 64-65 0.44468553673280553 1102 66-67 0.3919315022986609 1102 68-69 0.3632362359236865 1102 70-71 0.28370712198368864 1102 72-73 0.46415314279626685 1102 74-75 0.6204849159042958 1102 76-77 0.4658344044708045 1102 78-79 0.44632852859723116 1102 80-81 0.526835629609927 1102 82-83 0.43796133376014623 1102 84-85 0.5602364287373156 1102 86-87 0.7622822139424024 1102 88-89 0.7590532394678782 1102 90-91 0.8510718981779135 1102 92-93 0.7151509614471401 1102 94-95 0.6095050496928849 1102 96-97 0.4638031002219556 1102 98-99 0.690530686781198 1102 100-101 1.1923237611532365 1103 1 0.0019499349183220716 1103 2 0.043998335131362865 1103 3 0.07535402424905868 1103 4 0.08886247966464111 1103 5 0.10800981242496732 1103 6 0.0600917134940957 1103 7 0.07056526409521524 1103 8 0.07604767504697207 1103 9 -0.04992256449827437 1103 10-11 0.14930566120462885 1103 12-13 0.15377083056833385 1103 14-15 0.20383703294979227 1103 16-17 0.1619702286288529 1103 18-19 0.24649920827152982 1103 20-21 0.19382181571400992 1103 22-23 0.07687348974646824 1103 24-25 0.0748008585250588 1103 26-27 0.06042376741899602 1103 28-29 0.047985775689653565 1103 30-31 0.12365299004362384 1103 32-33 0.0670396576322716 1103 34-35 0.0011948481623065277 1103 36-37 0.03598481365150974 1103 38-39 0.04302132106829504 1103 40-41 0.1449463768218422 1103 42-43 0.28449561011087354 1103 44-45 0.2706815300154659 1103 46-47 0.32144705473018576 1103 48-49 0.2874632296714523 1103 50-51 0.3441929186066446 1103 52-53 0.39477052382962796 1103 54-55 0.41788840870248123 1103 56-57 0.16441353585672402 1103 58-59 0.21154637081990302 1103 60-61 0.2607605166049112 1103 62-63 0.42123425845962004 1103 64-65 0.2873440434215482 1103 66-67 0.5005341341562684 1103 68-69 0.45079034431052634 1103 70-71 0.5459510998686454 1103 72-73 0.6582482291611136 1103 74-75 0.4433014078518127 1103 76-77 0.29861060993667365 1103 78-79 0.42013469462694886 1103 80-81 0.5762583665236356 1103 82-83 0.7823353450487041 1103 84-85 0.612144381105896 1103 86-87 0.48185797851917656 1103 88-89 0.835490862003919 1103 90-91 0.5335370907219499 1103 92-93 0.620971884747199 1103 94-95 0.604881169162919 1103 96-97 0.615717710850145 1103 98-99 0.7763235651708982 1103 100-101 0.6661114765840317 1104 1 0.11466911637224086 1104 2 0.10192284449914268 1104 3 0.1062162268611857 1104 4 0.09365245027846925 1104 5 0.09306861243539544 1104 6 0.08890186172084924 1104 7 0.10787520016309315 1104 8 0.07728428078940652 1104 9 0.17794950172373802 1104 10-11 0.17874319918185932 1104 12-13 0.25027546369150855 1104 14-15 0.09660395607716055 1104 16-17 0.20951673172115903 1104 18-19 0.19783146329519496 1104 20-21 0.20706455944583269 1104 22-23 0.12194164529171303 1104 24-25 0.2136191375333354 1104 26-27 0.23685619540943037 1104 28-29 0.22985887386508352 1104 30-31 0.16379447670448144 1104 32-33 0.4594755022893082 1104 34-35 0.3118961893066654 1104 36-37 0.3210003127068646 1104 38-39 0.420653849251444 1104 40-41 0.4944593596018123 1104 42-43 0.48920048012504225 1104 44-45 0.5181859964141609 1104 46-47 0.4883686807916803 1104 48-49 0.4781802318546724 1104 50-51 0.42869615472090317 1104 52-53 0.6369741789675416 1104 54-55 0.7244729984614864 1104 56-57 0.7376379794038925 1104 58-59 0.6233115879844675 1104 60-61 0.6974830451910563 1104 62-63 0.6086464021650642 1104 64-65 0.5847935539548175 1104 66-67 0.6865081804487829 1104 68-69 0.7141320560099231 1104 70-71 0.6091712844350141 1104 72-73 0.8588323969728613 1104 74-75 0.7307782659408311 1104 76-77 0.6539079839866027 1104 78-79 0.7310639979161628 1104 80-81 0.8891377931178539 1104 82-83 0.9898749263095077 1104 84-85 0.6049673268192954 1104 86-87 0.5010502576744287 1104 88-89 0.8971591656257907 1104 90-91 0.9784607253788309 1104 92-93 0.9557807165617085 1104 94-95 0.9146216715962261 1104 96-97 0.8863154931775092 1104 98-99 1.0145562540732875 1104 100-101 1.8072883933821977 1105 1 -0.1922396805878357 1105 2 -0.11219068871441351 1105 3 -0.05025267999295835 1105 4 -0.03194338973059985 1105 5 -0.06617826569594598 1105 6 -0.030806577601261154 1105 7 -0.08675272851446181 1105 8 -0.005563411519460715 1105 9 -0.2325740260624798 1105 10-11 -0.22771633039256045 1105 12-13 -0.09263902546874192 1105 14-15 -0.14253236966733596 1105 16-17 -0.0731052934803742 1105 18-19 -0.0961428115731664 1105 20-21 -0.04175141545827188 1105 22-23 -0.2728533137888931 1105 24-25 -0.1987182240752361 1105 26-27 -0.043851716488539694 1105 28-29 -0.16123799228630276 1105 30-31 -0.056350752480057054 1105 32-33 -0.19658386622553792 1105 34-35 -0.026402381749001336 1105 36-37 -0.03387575792969244 1105 38-39 0.14139922433881225 1105 40-41 0.07599231901770764 1105 42-43 0.10255188928139347 1105 44-45 0.02692676936227656 1105 46-47 0.24221891530021367 1105 48-49 0.03740713063900358 1105 50-51 0.07048942742215303 1105 52-53 -0.012373736482309994 1105 54-55 -0.21681604451247694 1105 56-57 -0.10473875146970357 1105 58-59 -0.13477411499383862 1105 60-61 -0.30553184592915983 1105 62-63 -0.30739938651775134 1105 64-65 -0.07462632541891168 1105 66-67 -0.05478300590340979 1105 68-69 -0.34706407151801244 1105 70-71 -0.07858137584182856 1105 72-73 -0.1063101491614411 1105 74-75 -0.0463123124574345 1105 76-77 -0.02912847870445745 1105 78-79 -0.06326814456643604 1105 80-81 0.05770492181011022 1105 82-83 0.025241049757372735 1105 84-85 -0.0023795613399926197 1105 86-87 -0.011051026132580688 1105 88-89 0.1502852444913927 1105 90-91 0.2228012500012717 1105 92-93 0.2538959216102832 1105 94-95 0.0803633684934546 1105 96-97 0.04184207910515525 1105 98-99 0.4843154838043944 1105 100-101 1.1612504663045193 1106 1 -0.15117547592866032 1106 2 -0.16584821938842254 1106 3 -0.08974072880450734 1106 4 -0.10866101295732733 1106 5 -0.14013628945883028 1106 6 -0.07505190296107145 1106 7 -0.04179551746878474 1106 8 -0.04486420179596706 1106 9 -0.09729830871824419 1106 10-11 -0.18485497448913435 1106 12-13 -0.10056301821493463 1106 14-15 -0.11425557274353082 1106 16-17 0.004381454377146099 1106 18-19 -0.25223303531299734 1106 20-21 -0.08848758258688605 1106 22-23 9.982682803766352E-4 1106 24-25 -0.04705283612407385 1106 26-27 -0.014136743690308151 1106 28-29 0.04990775702076178 1106 30-31 -0.11708847529497746 1106 32-33 -0.3391827569272081 1106 34-35 -0.2418031453392686 1106 36-37 -0.4166261385118162 1106 38-39 -0.2665745776614443 1106 40-41 -0.1606725230786168 1106 42-43 -0.17766420858431076 1106 44-45 -0.3213524990709331 1106 46-47 -0.17419327181882238 1106 48-49 -0.25065828399839774 1106 50-51 -0.22611747824806372 1106 52-53 -0.0531779019612415 1106 54-55 -0.08734382549125286 1106 56-57 -0.08088754846629342 1106 58-59 -0.1360110068963749 1106 60-61 -0.2530736796375166 1106 62-63 -0.06791429441990715 1106 64-65 -0.3311121422906851 1106 66-67 -0.2040860554124464 1106 68-69 -0.05604497569623135 1106 70-71 -0.31249685325254006 1106 72-73 -0.37805147158576347 1106 74-75 -0.1462033689291502 1106 76-77 -0.3511161181248781 1106 78-79 -0.2444392609608812 1106 80-81 -0.19760010490645286 1106 82-83 -0.18254428069707984 1106 84-85 -0.4087556007385835 1106 86-87 -0.3277950475058269 1106 88-89 -0.44097903112891146 1106 90-91 -0.14780803013749733 1106 92-93 0.02717473516446134 1106 94-95 0.14567944877051175 1106 96-97 -0.2597271777438017 1106 98-99 -0.09071226381659159 1106 100-101 0.4582193798714371 1107 1 -0.12099590973214447 1107 2 -0.12223081983881912 1107 3 -0.18794506683935452 1107 4 -0.05881213302370014 1107 5 -0.10870035251441834 1107 6 -0.20720491943612274 1107 7 -0.2115774304710385 1107 8 -0.15651229946101353 1107 9 -0.1700878073552019 1107 10-11 -0.07822495196928259 1107 12-13 -0.11027026012557428 1107 14-15 -0.10613064682199536 1107 16-17 -0.1777108177575073 1107 18-19 -0.18622616083154497 1107 20-21 -0.17192215648756104 1107 22-23 -0.29264876265478534 1107 24-25 -0.18983314903150728 1107 26-27 -0.18748034923730472 1107 28-29 -0.25246868010142975 1107 30-31 -0.2869000196827898 1107 32-33 -0.1915843807271358 1107 34-35 -0.24949100832374427 1107 36-37 -0.1638763459315129 1107 38-39 -0.11905324306383847 1107 40-41 -0.2229621402411155 1107 42-43 -0.3260907820108869 1107 44-45 -0.2711287821099333 1107 46-47 -0.5585212319942343 1107 48-49 -0.6162873583139188 1107 50-51 -0.502430148628541 1107 52-53 -0.522509417902917 1107 54-55 -0.48194202390300944 1107 56-57 -0.3321602164762467 1107 58-59 -0.31507495142642483 1107 60-61 -0.417850851618077 1107 62-63 -0.5255458577718102 1107 64-65 -0.5909319834664544 1107 66-67 -0.2565558313991687 1107 68-69 -0.3417907976708605 1107 70-71 -0.24273568222017872 1107 72-73 -0.23333030292391754 1107 74-75 -0.408324087193936 1107 76-77 -0.5481440901528529 1107 78-79 -0.19904842298529957 1107 80-81 -0.20864520387528174 1107 82-83 -0.2640646670903486 1107 84-85 -0.2789197586146024 1107 86-87 -0.13599553346054805 1107 88-89 -0.10885120133158921 1107 90-91 0.06087841054046805 1107 92-93 -0.12609805137103436 1107 94-95 0.012003379077487608 1107 96-97 -0.3204581840256573 1107 98-99 -0.3097563553183065 1107 100-101 0.3822696939641226 1108 1 -0.048682908615731435 1108 2 -0.115503354672299 1108 3 -0.10758665533307266 1108 4 -0.021370309648659713 1108 5 -0.08255240627614313 1108 6 0.02744515088328825 1108 7 0.0832979519360606 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2316 72-73 -1.9527758605486802 2316 74-75 -2.2329928833090698 2316 76-77 -1.8551329635881757 2316 78-79 -2.0176777575130096 2316 80-81 -2.1587878219182315 2316 82-83 -2.414001019368701 2316 84-85 -2.4895265335609764 2316 86-87 -2.899613788570047 2316 88-89 -3.095518771638748 2316 90-91 -3.326963106632629 2316 92-93 -3.8462295899957724 2316 94-95 -4.024076039135185 2316 96-97 -4.3979940742686985 2316 98-99 -5.1074750559618565 2316 100-101 -5.4626673655111375 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 2.0 3 9.0 4 15.0 5 58.0 6 154.0 7 297.0 8 512.0 9 900.0 10 1489.0 11 2295.0 12 3076.0 13 4227.0 14 5126.0 15 7018.0 16 9281.0 17 13450.0 18 12851.0 19 8558.0 20 7419.0 21 7812.0 22 8509.0 23 9240.0 24 10876.0 25 12289.0 26 14862.0 27 17562.0 28 21174.0 29 25732.0 30 30929.0 31 37405.0 32 46071.0 33 58169.0 34 79456.0 35 116975.0 36 165016.0 37 250735.0 38 194854.0 39 13577.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.78435825348076 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17.848085944673535 31.243760329888648 32.08116996944857 18.82698375598925 20-21 20.279595652682016 27.673542129250905 31.198934873704072 20.84792734436301 22-23 18.98395632648291 27.91799529207499 32.59148733701731 20.50656104442478 24-25 21.37360389989816 28.401392343778692 31.399731214210586 18.825272542112558 26-27 19.939690145077545 26.610419205662865 34.98080101504198 18.4690896342176 28-29 18.022212390857945 30.78398637706806 32.53977528840215 18.654025943671847 30-31 19.272734102405717 28.005434147481594 31.796315464365016 20.925516285747676 32-33 19.460007679593982 28.51178650728727 32.880765956025975 19.147439857092774 34-35 21.25175194686269 27.729218091193896 32.08214345637934 18.936886505564075 36-37 20.025536717692795 26.893402778067642 34.219410409864736 18.861650094374827 38-39 19.11165821795668 29.043859824881284 33.199297721853775 18.64518423530826 40-41 18.8835793709856 29.443421635313893 32.544251508927516 19.12874748477299 42-43 18.439089663047778 29.588580386552632 30.435593760799485 21.53673618960011 44-45 19.641536460724524 27.4029319501091 31.29274925833115 21.662782330835224 46-47 21.544236658967574 29.327065544664045 31.178520121838734 17.950177674529645 48-49 20.32277775180805 28.66296987585728 31.31335686190708 19.70089551042759 50-51 17.286652718076112 29.636915099328405 33.43329405857657 19.643138124018915 52-53 19.380373628942053 31.143675186564053 29.429790146747024 20.046161037746874 54-55 18.426935341157616 28.773936125811783 30.84884555668709 21.95028297634351 56-57 20.61328235863704 28.32255129467938 29.991485667540356 21.072680679143225 58-59 22.262897258087065 28.099693024284676 29.965420886452378 19.671988831175877 60-61 19.848825391657847 29.595455579415713 32.21237903840094 18.3433399905255 62-63 18.50101838094125 29.695696088415502 32.01192841282826 19.79135711781499 64-65 18.212726387142308 31.65507141005805 30.36406003294725 19.76814216985239 66-67 19.647835093197905 29.16441805517122 28.884776347153057 22.302970504477816 68-69 18.489512357491815 29.21702147196374 30.644409338394723 21.64905683214972 70-71 21.173350139401325 29.189009833219252 29.39423028765088 20.243409739728545 72-73 19.94474031285998 27.549750413195547 32.20074625619793 20.30476301774654 74-75 18.208943388036527 32.10203843135945 29.382752633599896 20.306265547004124 76-77 19.03497163315868 29.04403514922626 29.67438618601477 22.24660703160029 78-79 19.361290441059854 29.06618485481369 30.566254138222902 21.00627056590355 80-81 20.74909430875307 29.046311290672634 29.4226531327735 20.7819412678008 82-83 19.558339872118065 28.41858795639326 31.53153641963973 20.491535751848946 84-85 18.749186129985475 29.529499657757224 30.645211105360694 21.07610310689661 86-87 18.731222256589454 30.289952601088423 29.772744371901727 21.206080770420403 88-89 18.535546998464923 29.78181934031035 28.689868063747255 22.992765597477476 90-91 19.46424069636649 27.726306126875993 29.607008222156523 23.202444954601 92-93 20.58611162122907 29.711681330239237 29.584008080268454 20.118198968263243 94-95 19.431417886776075 28.613582864488556 30.160228050551762 21.79477119818361 96-97 17.203991412153204 29.07042067761183 31.789056839391904 21.936531070843056 98-99 18.83862835773552 30.02946626821817 28.54934973872686 22.582555635319455 100-101 18.31264847935438 27.943877099579456 30.15648033603288 23.58699408503328 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 8818.0 1 5506.0 2 2204.5 3 2071.5 4 1722.0 5 1727.0 6 2887.0 7 5627.0 8 8098.5 9 8342.5 10 6256.0 11 4121.5 12 3524.0 13 3340.5 14 3311.0 15 3333.0 16 3336.5 17 3284.0 18 3229.5 19 3314.0 20 3488.5 21 3896.5 22 4427.0 23 5186.5 24 5956.5 25 6797.5 26 8259.5 27 9672.5 28 10559.0 29 12128.0 30 14962.0 31 17732.0 32 21422.5 33 26124.5 34 29878.0 35 33466.0 36 39676.0 37 44920.5 38 45138.0 39 43662.5 40 41253.5 41 37932.0 42 34520.5 43 34214.0 44 35289.0 45 35120.0 46 36208.5 47 58570.5 48 103662.5 49 97277.0 50 51880.5 51 30266.5 52 21452.5 53 17415.0 54 15019.0 55 13054.5 56 11412.0 57 10114.0 58 9308.0 59 8441.0 60 7170.5 61 5871.5 62 4744.0 63 3432.5 64 2177.5 65 1512.0 66 1015.0 67 697.5 68 551.5 69 411.0 70 260.5 71 161.0 72 139.5 73 135.0 74 112.0 75 78.5 76 55.0 77 26.0 78 10.0 79 4.0 80 1.0 81 0.5 82 0.5 83 1.0 84 1.5 85 1.5 86 0.5 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.3869012838277768 2 0.0 3 0.0 4 0.0 5 4.173692382176664E-4 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0012103707908312324 16-17 0.002295530810197165 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0022537938863753985 36-37 0.008096963221422728 38-39 0.01368971101353946 40-41 0.002212056962553632 42-43 5.008430858611997E-4 44-45 5.008430858611997E-4 46-47 2.5042154293059986E-4 48-49 3.3389539057413316E-4 50-51 7.930015526135662E-4 52-53 0.0 54-55 0.0 56-57 0.0 58-59 2.086846191088332E-4 60-61 0.002295530810197165 62-63 0.0 64-65 3.7563231439589976E-4 66-67 0.0015442661814053656 68-69 0.0018781615719794989 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.11890849596821315 78-79 1.6694769528706658E-4 80-81 0.0 82-83 0.0 84-85 0.0 86-87 0.14607923337618325 88-89 0.43464832467987774 90-91 0.334813602898212 92-93 0.0 94-95 0.0 96-97 6.677907811482663E-4 98-99 0.0 100-101 1.6694769528706658E-4 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1197980.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 33.95112108997853 #Duplication Level Percentage of deduplicated Percentage of total 1 73.84159117696184 25.07004803525722 2 13.217310462287713 8.974850159779406 3 4.834890342107162 4.92449842384944 4 2.2549943070032272 3.062383390971152 5 1.29601730439497 2.2000620218110596 6 0.8595015305924335 1.750862432529936 7 0.543206924959032 1.2909738860339284 8 0.41229314996682365 1.1198251727273847 9 0.31586488513418465 0.9651570266936575 >10 1.9809928617738892 13.256472017596382 >50 0.2550554082253159 6.028152730541486 >100 0.1708527066954467 10.331058180015093 >500 0.007118862775203115 1.517501006643832 >1k 0.0061369506682785475 4.904625260324191 >5k 0.002945736320773703 7.622258873276852 >10k+ 0.0012273901336557096 6.98127138194899 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 31511 2.630344413095377 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 17586 1.4679710846591763 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 12597 1.0515200587655888 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 11961 0.9984306916643015 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 10111 0.8440040735237649 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 9868 0.8237199285463865 No Hit GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT 8918 0.7444197732850298 No Hit ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC 8828 0.7369071269971118 No Hit TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC 8667 0.723467837526503 No Hit GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA 8093 0.6755538489791149 No Hit GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG 7660 0.6394096729494649 No Hit TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT 7397 0.6174560510192156 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7300 0.6093590877977929 No Hit ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC 6558 0.5474214928462913 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 6296 0.5255513447636856 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6164 0.5145327968747391 No Hit GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA 5708 0.47646872234928794 No Hit GATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATA 4645 0.3877360223042121 No Hit AAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGC 4560 0.38064074525451175 No Hit CCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCAC 4267 0.3561829078949565 No Hit TGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGAT 4238 0.35376216631329405 No Hit GTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTG 3729 0.3112739778627356 No Hit TACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACC 3354 0.2799712849964106 No Hit ATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATAC 3056 0.25509607839863774 No Hit GCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAG 2982 0.24891901367301625 No Hit CAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGT 2669 0.22279169936059032 No Hit ACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTG 2410 0.20117197282091523 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2254 0.18815005258852402 No Hit GCTTTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC 2064 0.1722900215362527 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1981 0.16536169218183944 No Hit AGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAG 1642 0.13706405783068165 No Hit CTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCT 1625 0.13564500242074157 No Hit GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 1565 0.13063657156212957 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1494 0.12470992837943873 No Hit AACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACG 1489 0.12429255914122106 No Hit AGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTA 1373 0.1146095928145712 No Hit CACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACT 1347 0.11243927277583933 No Hit GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA 1317 0.10993505734653335 No Hit ATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCA 1269 0.10592831265964374 No Hit ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1217 0.10158767258218 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 8.347384764353328E-5 0.0 9 0.0 0.0 0.0 8.347384764353328E-5 0.0 10-11 0.0 0.0 0.0 1.6694769528706655E-4 0.0 12-13 0.0 0.0 0.0 3.338953905741331E-4 0.0 14-15 0.0 0.0 0.0 3.338953905741331E-4 0.0 16-17 0.0 0.0 0.0 5.008430858611997E-4 0.0 18-19 0.0 0.0 0.0 5.425800096829664E-4 0.0 20-21 0.0 0.0 0.0 9.599492479006328E-4 0.0 22-23 0.0 0.0 0.0 0.0014190554099400656 0.0 24-25 0.0 0.0 0.0 0.0026294262007712987 0.0 26-27 0.0 0.0 0.0 0.004507587772750797 0.0 28-29 0.0 0.0 0.0 0.012854972537104126 0.0 30-31 0.0 0.0 0.0 0.03626938680111521 0.0 32-33 0.0 0.0 0.0 0.07571077981268469 0.0 34-35 0.0 0.0 0.0 0.13042788694302077 0.0 36-37 0.0 0.0 0.0 0.2038013990216865 0.0 38-39 0.0 0.0 0.0 0.3151555117781599 0.0 40-41 0.0 0.0 0.0 0.43531611546102605 0.0 42-43 0.0 0.0 0.0 0.5643249469941067 0.0 44-45 0.0 0.0 0.0 0.7180420374296733 0.0 46-47 0.0 0.0 0.0 0.9086128315998597 0.0 48-49 0.0 0.0 0.0 1.0836157531845272 0.0 50-51 0.0 0.0 0.0 1.2736022304212091 0.0 52-53 0.0 0.0 0.0 1.464799078448722 0.0 54-55 0.0 0.0 0.0 1.6774904422444448 0.0 56-57 0.0 0.0 0.0 1.952536770229887 0.0 58-59 0.0 0.0 0.0 2.2187348703651146 0.0 60-61 0.0 0.0 0.0 2.525918629693317 0.0 62-63 0.0 0.0 0.0 2.828260905858195 0.0 64-65 0.0 0.0 0.0 3.1473814253994226 0.0 66-67 0.0 0.0 0.0 3.5289403829780133 0.0 68-69 0.0 0.0 0.0 3.9696822985358686 0.0 70-71 0.0 0.0 0.0 4.423738292792868 0.0 72-73 0.0 0.0 0.0 4.864438471426903 0.0 74-75 0.0 0.0 0.0 5.377009632882018 0.0 76-77 0.0 0.0 0.0 5.851808878278435 0.0 78-79 0.0 0.0 0.0 6.51200353929114 0.0 80-81 0.0 0.0 0.0 7.20775805939999 0.0 82-83 0.0 0.0 0.0 7.826716639676789 0.0 84-85 0.0 0.0 0.0 8.508489290305347 0.0 86-87 0.0 0.0 0.0 9.200696171889348 0.0 88-89 0.0 0.0 0.0 9.956677073073006 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position ACGGTCG 25 0.0016236722 37.964172 46-47 GTAAGCG 250 0.0 29.422234 94-95 CTTTGCG 705 0.0 28.273552 2 TAGACCC 195 5.4023985E-10 26.771841 5 CATATAG 305 0.0 26.452656 3 TTTGCGT 755 0.0 25.772533 3 TAGACGC 95 0.0012327478 24.978516 4 TGGGACG 95 0.0012333479 24.976429 6 TACAATC 295 0.0 24.129772 7 TTGCGTT 820 0.0 23.729588 4 GCTTTGC 865 0.0 23.132597 1 GAGTACT 7075 0.0 23.108131 12-13 CCTGTAC 295 5.456968E-12 22.523 3 AGTACTT 7600 0.0 22.136284 12-13 CTCCCGC 195 4.2602733E-7 21.902407 7 GTATAGG 675 0.0 21.88009 1 ACTATAC 545 0.0 21.770264 3 GTACATG 6030 0.0 21.72734 1 GTATAGA 220 5.464244E-8 21.655516 1 TCTAGAC 220 5.691254E-8 21.572355 3 >>END_MODULE