FastQCFastQC Report
Thu 26 May 2016
SRR938881_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938881_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2185913
Sequences flagged as poor quality0
Sequence length101
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT185600.8490731332857255No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT145420.6652597793233308No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT98780.45189355660540925No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT66890.30600485929677895No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT44720.20458270754600025No Hit
GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA41110.18806786912379403No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT35850.164004697350718No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA33680.15407749530745277No Hit
GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA33190.15183586903961868No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT31710.1450652427612627No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT31320.14328109124196617No Hit
CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC29250.13381136394723853No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT28480.13028880838349924No Hit
GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT24920.11400270733556185No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTTGCG4950.023.9282262
TTTGCGT4400.023.6889423
GAGTACT108850.023.55953612-13
GTACATG110550.023.3578011
TACATGG114650.022.1496262
AGTACTT113250.021.61966912-13
GTACTTT118250.021.58660714-15
ACATGGG114750.021.1394143
GTATCAA257100.020.7702751
TAGACCG1605.091876E-520.7278255
GGTATCA201600.019.6366461
GTATAGG6550.019.566551
ACTTTTT134950.019.19733616-17
CATGGGA68600.018.9925924
TACTTTT130250.018.6706514-15
CATGGGG47000.018.4471344
CGGTTAC2350.018.1792728-29
TATCAAC294050.018.0134262
CAACGCA295500.017.9095
ATCAACG295800.017.874823
Produced by FastQC (version 0.11.5)