##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938881_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2185913 Sequences flagged as poor quality 0 Sequence length 101 %GC 43 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.320671042260145 33.0 31.0 34.0 31.0 34.0 2 32.43508318949565 34.0 31.0 34.0 31.0 34.0 3 32.467882299066794 34.0 31.0 34.0 31.0 34.0 4 36.000146849394284 37.0 35.0 37.0 35.0 37.0 5 36.034964795030724 37.0 35.0 37.0 35.0 37.0 6 36.12964742878605 37.0 37.0 37.0 35.0 37.0 7 36.08059973109634 37.0 37.0 37.0 35.0 37.0 8 36.077348915533236 37.0 37.0 37.0 35.0 37.0 9 37.77806207291873 39.0 38.0 39.0 35.0 39.0 10-11 37.69083444766558 39.0 38.0 39.0 35.0 39.0 12-13 37.60300135458273 39.0 38.0 39.0 35.0 39.0 14-15 38.898555660723915 40.0 38.0 41.0 36.0 41.0 16-17 38.827625802124786 40.0 38.0 41.0 36.0 41.0 18-19 38.790044937744554 40.0 38.0 41.0 35.0 41.0 20-21 38.90754641195693 40.0 39.0 41.0 36.0 41.0 22-23 38.850214761520704 40.0 39.0 41.0 35.0 41.0 24-25 38.77611986387382 40.0 38.0 41.0 35.0 41.0 26-27 38.66263044320611 40.0 38.0 41.0 35.0 41.0 28-29 38.53613981892235 40.0 38.0 41.0 35.0 41.0 30-31 38.328427297884225 40.0 38.0 41.0 34.5 41.0 32-33 38.100660227557086 40.0 38.0 41.0 34.0 41.0 34-35 37.796213527253826 40.0 37.0 41.0 33.5 41.0 36-37 37.54116334913604 40.0 37.0 41.0 33.0 41.0 38-39 37.31510357457044 40.0 36.5 41.0 33.0 41.0 40-41 37.16498781058533 40.0 36.5 41.0 32.5 41.0 42-43 36.947807620888845 40.0 36.0 41.0 31.5 41.0 44-45 36.837263651389605 40.0 36.0 41.0 31.0 41.0 46-47 36.54736739293833 40.0 36.0 41.0 30.5 41.0 48-49 36.25743270660818 40.0 36.0 41.0 30.0 41.0 50-51 35.14574802382346 38.0 34.5 40.0 28.0 40.5 52-53 35.25254184407156 38.5 35.0 39.5 28.0 40.5 54-55 35.7386801304535 39.0 35.0 40.5 29.0 41.0 56-57 35.64988977145934 39.0 35.0 41.0 28.5 41.0 58-59 35.440438388902024 39.0 35.0 41.0 28.0 41.0 60-61 35.135254239304125 38.0 35.0 40.0 28.0 41.0 62-63 34.771753724873776 38.0 35.0 40.0 26.5 41.0 64-65 34.38872452837785 37.0 34.0 40.0 26.0 41.0 66-67 33.98602551885642 37.0 34.0 39.5 26.0 41.0 68-69 33.55721522311272 36.0 34.0 39.0 25.0 41.0 70-71 33.107897249341576 35.5 33.0 39.0 23.5 40.0 72-73 32.675874337176275 35.0 33.0 38.0 22.5 40.0 74-75 32.22022765773386 35.0 32.5 37.0 20.5 39.5 76-77 31.8043876860607 35.0 32.0 37.0 20.0 39.0 78-79 31.435569713890718 35.0 32.0 36.0 18.5 39.0 80-81 31.0040358422316 35.0 31.5 36.0 15.5 37.5 82-83 30.56473976777667 35.0 31.0 35.0 9.0 37.0 84-85 30.17436627166772 34.0 31.0 35.0 6.0 37.0 86-87 29.7638302622291 34.0 30.5 35.0 2.0 36.0 88-89 29.320254054026854 34.0 30.0 35.0 2.0 36.0 90-91 28.90411626629239 34.0 29.0 35.0 2.0 35.5 92-93 28.44850755725411 34.0 29.0 35.0 2.0 35.0 94-95 27.9654874187582 34.0 29.0 35.0 2.0 35.0 96-97 27.38399332452847 34.0 27.0 35.0 2.0 35.0 98-99 26.610152371114495 33.0 25.5 35.0 2.0 35.0 100-101 23.48342751975948 30.0 12.5 33.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.10395391570833112 1101 2 0.19553093393705723 1101 3 0.2654235107809697 1101 4 0.1512276111113664 1101 5 0.09423563896142895 1101 6 0.17318029858363104 1101 7 0.23581808741289478 1101 8 0.2547599056672212 1101 9 0.30807813329725064 1101 10-11 0.17929709362400814 1101 12-13 0.19747912241849264 1101 14-15 0.3210485554706679 1101 16-17 0.2926178241980679 1101 18-19 0.38459798600078443 1101 20-21 0.29977373936533525 1101 22-23 0.3642820253041492 1101 24-25 0.3302453208740701 1101 26-27 0.38634530598838523 1101 28-29 0.23703584732021454 1101 30-31 0.23326747186219166 1101 32-33 0.19172530989020942 1101 34-35 0.1886970839287656 1101 36-37 0.11958614755559438 1101 38-39 0.1293562660774441 1101 40-41 0.003005420656734259 1101 42-43 0.09200832508837209 1101 44-45 0.13400696433760118 1101 46-47 0.10560130600357098 1101 48-49 0.1719039738705206 1101 50-51 0.08257345109723957 1101 52-53 0.12950740178276732 1101 54-55 0.1905838444930552 1101 56-57 0.14120728432042995 1101 58-59 0.1218648429822764 1101 60-61 0.03986427090994482 1101 62-63 0.006642226114713878 1101 64-65 -0.1338034032669455 1101 66-67 -0.11570585359010721 1101 68-69 -0.16169387613105357 1101 70-71 -0.29623274093201957 1101 72-73 -0.24063470602401082 1101 74-75 -0.3411790632193288 1101 76-77 -0.44559685490544254 1101 78-79 -0.4898132999917628 1101 80-81 -0.4927569113494421 1101 82-83 -0.6567481309223453 1101 84-85 -0.6182262373862422 1101 86-87 -0.7080066227627526 1101 88-89 -1.004803917444363 1101 90-91 -0.8947983961895645 1101 92-93 -0.9057583046717959 1101 94-95 -1.0564328823826301 1101 96-97 -1.344225711847585 1101 98-99 -1.3343959655348314 1101 100-101 -0.9903727629547348 1102 1 0.22961430129358007 1102 2 0.19465625541774756 1102 3 0.2285447565944878 1102 4 0.13068411688519177 1102 5 0.16769762402802968 1102 6 0.14013255703572014 1102 7 0.2631293296083399 1102 8 0.17669396697443318 1102 9 0.2295825627335759 1102 10-11 0.32810034534930566 1102 12-13 0.238840273699779 1102 14-15 0.2996204732253389 1102 16-17 0.38635002150633824 1102 18-19 0.2907761627717278 1102 20-21 0.2687788057622882 1102 22-23 0.2713992923433679 1102 24-25 0.19862183870665717 1102 26-27 0.2919568795795229 1102 28-29 0.2948666974429983 1102 30-31 0.3158940926985281 1102 32-33 0.3186891250212227 1102 34-35 0.2717707333134811 1102 36-37 0.23213197073444292 1102 38-39 0.34317354414923784 1102 40-41 0.275329596951579 1102 42-43 0.28246770087356055 1102 44-45 0.3858837685547343 1102 46-47 0.4283575695231576 1102 48-49 0.39221935141036113 1102 50-51 0.19609998444369836 1102 52-53 0.3079333415942287 1102 54-55 0.22278850781560067 1102 56-57 0.1276298381659018 1102 58-59 0.059696824990147945 1102 60-61 0.2599744116975131 1102 62-63 0.22642744541304438 1102 64-65 0.1752153206229039 1102 66-67 0.1058700123295182 1102 68-69 0.12105674557996338 1102 70-71 -0.02661451872712206 1102 72-73 -0.07061807788328167 1102 74-75 -0.2457092901666904 1102 76-77 -0.18670786838962883 1102 78-79 -0.21020255835818702 1102 80-81 -0.29650179942384725 1102 82-83 -0.16289433304046597 1102 84-85 -0.24624761636141201 1102 86-87 -0.35917020724465587 1102 88-89 -0.4282334323831556 1102 90-91 -0.42080730989477644 1102 92-93 -0.39006628135672017 1102 94-95 -0.6358250424708203 1102 96-97 -0.6684446175437238 1102 98-99 -0.6382544825407415 1102 100-101 -0.5814902836771516 1103 1 0.05598504562632911 1103 2 0.12996590834398347 1103 3 0.15533066179144583 1103 4 0.10418177000287443 1103 5 0.03395172430631277 1103 6 0.04781551566858866 1103 7 -0.0043172901698866895 1103 8 0.028198248530529213 1103 9 0.12421888655815394 1103 10-11 0.1461479665057297 1103 12-13 0.12170516994981995 1103 14-15 0.14475967803640089 1103 16-17 0.09727665643988104 1103 18-19 0.1872136343387325 1103 20-21 0.2022406774797716 1103 22-23 0.1659566635846801 1103 24-25 0.2057080008014509 1103 26-27 0.20935816312934463 1103 28-29 0.06743326923236737 1103 30-31 0.05800151831199685 1103 32-33 0.046365561678655354 1103 34-35 -0.10108340033512775 1103 36-37 0.003974437740986048 1103 38-39 0.07631806226785187 1103 40-41 -0.07750088670791655 1103 42-43 -0.21960452486015214 1103 44-45 -0.03529393112037127 1103 46-47 0.09828533573762854 1103 48-49 -0.11826177176507002 1103 50-51 -0.2423150994808836 1103 52-53 -0.21783819065399257 1103 54-55 -0.10871795667877393 1103 56-57 -0.26447060920224885 1103 58-59 -0.3037770484146378 1103 60-61 -0.28266076608447577 1103 62-63 -0.3668165619069583 1103 64-65 -0.3893369153455737 1103 66-67 -0.4639108752406642 1103 68-69 -0.44543005234034183 1103 70-71 -0.41963958360020115 1103 72-73 -0.3848300125615367 1103 74-75 -0.4547825523487603 1103 76-77 -0.261791551418046 1103 78-79 -0.5160849955014193 1103 80-81 -0.6089446818149149 1103 82-83 -0.5238865679532516 1103 84-85 -0.6184799704590489 1103 86-87 -0.4963752589574746 1103 88-89 -0.4034728510595329 1103 90-91 -0.3964353407905108 1103 92-93 -0.6041685770652343 1103 94-95 -0.6356005979458565 1103 96-97 -0.6244355977143243 1103 98-99 -0.5938636721741126 1103 100-101 -0.2975635981960636 1104 1 0.10361425218128062 1104 2 0.12557088262194327 1104 3 0.06742249600018368 1104 4 0.13953325954078366 1104 5 0.07743569922788396 1104 6 0.16501530143077758 1104 7 0.05668512675669746 1104 8 0.0626009706109798 1104 9 0.1334117591001629 1104 10-11 0.1850841137357193 1104 12-13 0.19592349448409863 1104 14-15 0.22287726579063616 1104 16-17 0.2573633867477767 1104 18-19 0.20194608461621044 1104 20-21 0.24659645627473026 1104 22-23 0.2991158161751031 1104 24-25 0.20883942849844317 1104 26-27 0.16554518013784048 1104 28-29 0.27483122497154966 1104 30-31 0.2565664400206771 1104 32-33 0.2224060328218158 1104 34-35 0.20896057242606503 1104 36-37 0.18542171318510015 1104 38-39 0.17867760372691777 1104 40-41 0.17162753445841616 1104 42-43 0.09605193850563154 1104 44-45 0.2325940261040742 1104 46-47 0.2553239030447543 1104 48-49 0.001810871056392216 1104 50-51 -0.03340735226350233 1104 52-53 0.048661721580515405 1104 54-55 0.0697083855706282 1104 56-57 0.06370661407765965 1104 58-59 -0.054038544568712155 1104 60-61 -0.1207689921863846 1104 62-63 0.026236912031194493 1104 64-65 0.08294291704483925 1104 66-67 -0.06418744905594309 1104 68-69 0.03351073070786015 1104 70-71 -0.10671600258815062 1104 72-73 -0.029455388940469618 1104 74-75 -0.0631195988374671 1104 76-77 -0.09005721530771993 1104 78-79 -0.08607234826918031 1104 80-81 -0.19310233564081258 1104 82-83 -0.005471567705711777 1104 84-85 0.008213565950125457 1104 86-87 0.07329703820296984 1104 88-89 -0.008944466799711392 1104 90-91 -0.14427383428461482 1104 92-93 -0.12586758193959469 1104 94-95 -0.18331194399843298 1104 96-97 -0.3175280035239467 1104 98-99 -0.04051733154499004 1104 100-101 0.1706659693226058 1105 1 0.17109782306872034 1105 2 0.16382032323929252 1105 3 0.2400929095028843 1105 4 0.20004789158151226 1105 5 0.19758492666318261 1105 6 0.17181774685793272 1105 7 0.13588886537083766 1105 8 0.1695932537731153 1105 9 0.173273278859142 1105 10-11 0.2697704630371547 1105 12-13 0.1482654138272892 1105 14-15 0.29300469051622713 1105 16-17 0.3090584923697932 1105 18-19 0.30639714083636704 1105 20-21 0.30135451226585275 1105 22-23 0.2624282423982649 1105 24-25 0.3373223563160579 1105 26-27 0.23230331541154214 1105 28-29 0.21102592537148723 1105 30-31 0.23849771964152922 1105 32-33 0.30176613430352006 1105 34-35 0.36741892264365816 1105 36-37 0.296873201054872 1105 38-39 0.3260877060895382 1105 40-41 0.43695131570896706 1105 42-43 0.34796242847198045 1105 44-45 0.3292745084945281 1105 46-47 0.34551620734891486 1105 48-49 0.257696721758478 1105 50-51 0.30573159486382906 1105 52-53 0.26862573609803775 1105 54-55 0.3541143828574107 1105 56-57 0.33670054455564724 1105 58-59 0.22859042176261113 1105 60-61 0.32226669378412964 1105 62-63 0.32623294790215596 1105 64-65 0.31099771892647965 1105 66-67 0.32816112121485475 1105 68-69 0.34406720633287335 1105 70-71 0.17984493928890544 1105 72-73 0.18378766502453914 1105 74-75 0.2591819304354246 1105 76-77 0.1830893341381845 1105 78-79 0.15829088046987394 1105 80-81 0.26857136368440493 1105 82-83 0.2836598916223245 1105 84-85 0.34061327348549497 1105 86-87 0.2885217162276028 1105 88-89 0.25950635157270696 1105 90-91 0.32809948976972336 1105 92-93 0.437989251337946 1105 94-95 0.312835363036573 1105 96-97 0.11202479867482396 1105 98-99 0.12323621414819996 1105 100-101 0.5652804797967157 1106 1 0.1342988501138933 1106 2 0.08849671876997434 1106 3 0.10934644040556663 1106 4 0.13264935665171151 1106 5 0.1242987996011351 1106 6 0.14632253329899214 1106 7 0.1486036376691473 1106 8 0.15352371077690918 1106 9 0.13735714029849788 1106 10-11 0.13074242021577476 1106 12-13 0.2698240992651293 1106 14-15 0.2746931613078871 1106 16-17 0.18370307541972153 1106 18-19 0.23759379024065197 1106 20-21 0.2084773984645878 1106 22-23 0.25123277155614687 1106 24-25 0.10455295700875666 1106 26-27 0.10888937306454238 1106 28-29 0.08801121552393454 1106 30-31 0.20875391992229453 1106 32-33 0.20322181079820467 1106 34-35 0.10726665328087392 1106 36-37 0.09633127990284862 1106 38-39 0.36467061337192774 1106 40-41 0.4215035621093506 1106 42-43 0.2584124696129422 1106 44-45 0.25706115568954857 1106 46-47 0.13664433053867242 1106 48-49 0.23924309100418384 1106 50-51 0.11462871725859713 1106 52-53 0.10718044051812115 1106 54-55 0.27544992429147896 1106 56-57 0.12580456332218404 1106 58-59 0.243122289039988 1106 60-61 0.4155541298764831 1106 62-63 0.28346744959215897 1106 64-65 0.22909123587777458 1106 66-67 0.06222498188879655 1106 68-69 0.22051714222952512 1106 70-71 0.25038047185114465 1106 72-73 0.06767982963010155 1106 74-75 0.01084376833137668 1106 76-77 -0.03230594952563948 1106 78-79 0.04499680789827565 1106 80-81 0.13570293906106556 1106 82-83 0.1768065960841625 1106 84-85 0.18598004551243008 1106 86-87 0.2344699629967728 1106 88-89 0.039215685959060664 1106 90-91 0.06681736633990809 1106 92-93 0.3228515552660802 1106 94-95 0.15823431720398418 1106 96-97 0.41760063719353724 1106 98-99 0.3188508973802655 1106 100-101 0.4349347653271032 1107 1 0.15932923230384688 1107 2 0.22093049930857234 1107 3 0.14915782228526808 1107 4 0.18844781260225574 1107 5 0.15718805587597728 1107 6 0.13855964807939358 1107 7 0.11905470820211406 1107 8 0.2333264536587336 1107 9 0.18637124037717712 1107 10-11 0.22205354241130948 1107 12-13 0.26065341306473044 1107 14-15 0.3000472916573429 1107 16-17 0.3177521685820537 1107 18-19 0.34388335350845267 1107 20-21 0.28375098815674704 1107 22-23 0.3403309004589161 1107 24-25 0.28862585455264167 1107 26-27 0.2214070710115834 1107 28-29 0.19879265059174855 1107 30-31 0.3250361811799962 1107 32-33 0.3392617087414749 1107 34-35 0.277240436322046 1107 36-37 0.33577304037292066 1107 38-39 0.4333846068343945 1107 40-41 0.34277790857087354 1107 42-43 0.3812128676239084 1107 44-45 0.12610716778072373 1107 46-47 0.27953618365514643 1107 48-49 0.25783204182874897 1107 50-51 0.28390251007005674 1107 52-53 0.3116439013281038 1107 54-55 0.29941018061602875 1107 56-57 0.21691760135263394 1107 58-59 0.26226724679631985 1107 60-61 0.24711809702772314 1107 62-63 0.2738359954122629 1107 64-65 0.2831464654711411 1107 66-67 0.22055358005712122 1107 68-69 0.2764190861754656 1107 70-71 0.009042074248121423 1107 72-73 0.23897442374289568 1107 74-75 0.2289272563610396 1107 76-77 0.30220078340762413 1107 78-79 0.27987213378904485 1107 80-81 0.23163834626811663 1107 82-83 0.2870956596956411 1107 84-85 0.30141726193818386 1107 86-87 0.4352486726664573 1107 88-89 0.4547553405019933 1107 90-91 0.3541073264188235 1107 92-93 0.42991770702105114 1107 94-95 0.4201075827623235 1107 96-97 0.47788260877054967 1107 98-99 0.5351130609692873 1107 100-101 0.5547970041645414 1108 1 0.12005075561410905 1108 2 0.027751695150122657 1108 3 0.09506047618602764 1108 4 0.08254683158295961 1108 5 0.16341910594280762 1108 6 0.13194244843095504 1108 7 0.20652105554777478 1108 8 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-1.1433324323892684 2316 92-93 -1.0142733506466506 2316 94-95 -1.087285620011201 2316 96-97 -1.460061638314869 2316 98-99 -1.3250517931238406 2316 100-101 -1.2460595390442144 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 5398.0 3 1181.0 4 1090.0 5 1194.0 6 1266.0 7 1541.0 8 1807.0 9 2259.0 10 2823.0 11 4013.0 12 6145.0 13 10926.0 14 9506.0 15 10140.0 16 14820.0 17 22831.0 18 19082.0 19 10968.0 20 10067.0 21 10366.0 22 10671.0 23 11785.0 24 13068.0 25 14885.0 26 17497.0 27 21371.0 28 25688.0 29 31339.0 30 39263.0 31 51183.0 32 67057.0 33 92142.0 34 133950.0 35 211338.0 36 319904.0 37 484406.0 38 428317.0 39 64618.0 40 8.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.68334128527911 19.33506441076082 15.078357250339486 21.903237053620582 2 15.060784832588764 20.94327511992609 40.737466476091235 23.25847357139391 3 18.928793817076297 25.87931760741365 30.86906157283616 24.322827002673893 4 12.42990470198824 17.208175308414734 37.890128668112645 32.47179132148438 5 13.043366766356698 37.87396927055929 34.20476250006296 14.877901463021043 6 30.04964076126208 36.59662830942222 17.85264117592675 15.501089753388944 7 27.009063222867773 34.263714978529656 21.179665280469262 17.547556518133312 8 25.72561957144106 34.21921892370988 20.15152479086732 19.90363671398174 9 26.604101233702217 17.2259688045529 21.133501098037996 35.036428863706895 10-11 24.433893954608994 26.77729424332605 27.25492690991277 21.533884892152187 12-13 26.374881257264448 24.757737357889177 27.749951312903896 21.11743007194248 14-15 23.046639988045197 25.442635586004 27.402386063772845 24.10833836217796 16-17 21.017850498130553 29.030870958396132 27.837755947839437 22.11352259563388 18-19 20.891354065133964 27.326686176203292 30.818514850828027 20.96344490783472 20-21 22.14766068353612 26.116973604589745 30.578711565626005 21.15665414624813 22-23 21.71869088005838 26.16932878315552 30.666982240069107 21.44499809671699 24-25 21.708519546006958 26.294324491803362 30.764115403289548 21.23304055890013 26-27 21.507552894986468 26.203550931911323 31.04716874022583 21.24172743287638 28-29 20.913417519392336 26.74991118712598 31.10609109820323 21.23058019527845 30-31 21.193863002712078 26.522410166041162 30.85202783168691 21.43169899955985 32-33 21.03019743074315 26.647943516538998 30.799864233893054 21.5219948188248 34-35 21.643529988218326 26.508999585255655 30.527890639623866 21.319579786902153 36-37 21.823040987186637 26.14905906131524 30.705570179640745 21.32232977185738 38-39 21.54203813887228 26.44653935639533 30.827945536372823 21.18347696835956 40-41 21.400360979573353 26.676225115478786 30.760636185733382 21.16277771921448 42-43 21.249669482564183 26.87711505314812 30.105882739420398 21.767332724867295 44-45 21.320774137104735 26.90608448164712 29.93072668515977 21.842414696088376 46-47 21.72913895006866 27.006547683729025 29.602463864371675 21.661849501830645 48-49 21.801128962622272 27.150592915618237 29.289335331125837 21.758942790633657 50-51 21.454045819767614 27.46132477994242 29.444014273011554 21.64061512727841 52-53 21.69444435509182 27.638183449923194 29.047256458318305 21.620115736666687 54-55 21.224913087788916 27.371717760233494 29.209096581682775 22.194272570294817 56-57 21.63127319785712 27.760779000092107 28.714798231616683 21.893149570434087 58-59 21.989423705396042 27.251366820492823 28.866541456126015 21.89266801798512 60-61 21.700567513958273 27.193974066999704 29.509417242139293 21.596041176902734 62-63 21.68937467408209 27.30997980744712 29.398105713412768 21.602539805058015 64-65 21.411031202170385 27.51529915761286 29.33543533752126 21.738234302695496 66-67 21.406689739912387 27.539100694931967 28.898447462100002 22.155762103055647 68-69 21.161929766742553 27.69455855199926 29.187494455498975 21.95601722575921 70-71 21.568239016330978 27.465532722282372 28.879178893852664 22.087049367533986 72-73 21.465791129394365 27.37376044575926 28.93570104642575 22.224747378420624 74-75 21.2144470314426 28.107745153821195 28.627751698426078 22.05005611631013 76-77 21.462978461988047 27.50634487882643 28.748923375091866 22.281753284093657 78-79 21.43383148435099 27.555066352502756 28.841758687016572 22.169343476129683 80-81 21.487573120405028 27.857619510872873 28.608573105473706 22.04623426324839 82-83 21.480671989759667 27.66052612656654 28.779827534359768 22.078974349314024 84-85 21.419424707685636 27.630048189139618 28.48799222945529 22.46253487371946 86-87 21.446260980157376 27.534637060943034 28.794156107306197 22.224945851593393 88-89 21.615817739703242 27.375298440116236 28.519663504748394 22.489220315432135 90-91 21.412946622445403 27.33633360247828 28.73569583465646 22.515023940419855 92-93 21.554801741625756 27.524727721611953 28.635326655937 22.285143880825295 94-95 21.50593825210564 27.235941908288414 28.531301497656113 22.726818341949837 96-97 21.263014846305843 27.105784955709712 28.6407159058765 22.990484292107944 98-99 21.31325349932532 27.338352679047436 28.33387258668804 23.014521234939206 100-101 21.55520342893824 26.597177182103298 29.00052175699082 22.847097631967646 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 6089.0 1 3807.5 2 1796.0 3 2012.0 4 1653.5 5 1666.5 6 2936.0 7 6151.5 8 9966.0 9 10864.0 10 8130.5 11 5558.5 12 5159.5 13 5307.5 14 5407.0 15 5519.5 16 5692.5 17 5740.5 18 5783.5 19 6000.0 20 6310.0 21 6835.0 22 7713.5 23 8856.5 24 9963.0 25 11790.5 26 14460.5 27 16928.5 28 19000.5 29 21796.5 30 25229.5 31 28487.0 32 32128.0 33 36891.0 34 41676.5 35 45063.5 36 50021.5 37 55406.5 38 57594.5 39 58598.0 40 59547.0 41 61221.0 42 63442.0 43 66712.0 44 71207.5 45 73770.5 46 74163.0 47 80518.5 48 102805.0 49 102940.5 50 81817.0 51 73989.5 52 69925.0 53 68310.5 54 66316.5 55 61507.0 56 55858.0 57 50158.0 58 46569.5 59 42460.5 60 36195.5 61 29477.0 62 23461.5 63 18450.0 64 13584.5 65 9904.0 66 7062.0 67 5348.5 68 3894.5 69 2482.0 70 1697.0 71 1181.0 72 850.5 73 617.0 74 456.0 75 332.0 76 235.5 77 142.0 78 78.5 79 54.5 80 47.5 81 38.0 82 28.5 83 21.5 84 15.5 85 13.0 86 8.5 87 9.5 88 11.0 89 7.5 90 3.0 91 4.5 92 5.5 93 2.0 94 1.5 95 2.0 96 1.5 97 1.5 98 1.5 99 0.5 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.28148421277516533 2 0.0755290809835524 3 0.08385512140693614 4 0.0879266466689205 5 0.09808258608645448 6 0.04643368697656311 7 0.047531626373053276 8 0.048309333445567135 9 0.05064245466310873 10-11 0.04917853546778852 12-13 0.04769174253504142 14-15 0.0465480556636975 16-17 0.04776036374732206 18-19 0.05350167184146853 20-21 0.055583181947314465 22-23 0.06997076278882097 24-25 0.07751909613969082 26-27 0.07250974764320446 28-29 0.0706798486490542 30-31 0.06516727792917651 32-33 0.05825940922625923 34-35 0.28624195015995607 36-37 0.4441393596176975 38-39 0.4189325009732775 40-41 0.40173145042826497 42-43 0.4300948848375942 44-45 0.43945024344518746 46-47 0.4343493999989936 48-49 0.45036101619780844 50-51 0.4473188091200336 52-53 0.4467698394217885 54-55 0.438215061624136 56-57 0.41733133935339606 58-59 0.40166282921598434 60-61 0.3656595665060778 62-63 0.42856234442999336 64-65 0.4526483899404962 66-67 0.46152340006212506 68-69 0.47275440513872236 70-71 0.4694834606866788 72-73 0.45884717278318027 74-75 0.46090580915159934 76-77 0.4369570060656577 78-79 0.4176744454147992 80-81 0.4245594403802896 82-83 0.43100983433466933 84-85 0.4340977888872979 86-87 0.4340062939375904 88-89 0.43903851617150363 90-91 0.4522595364042393 92-93 0.458160960660374 94-95 0.46417675360364297 96-97 0.45676566267733437 98-99 0.46044833440306177 100-101 0.43974760203173685 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2185913.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 46.662905812110274 #Duplication Level Percentage of deduplicated Percentage of total 1 72.08603304869939 33.63743770520128 2 14.459193685967826 13.494159861747523 3 5.2626244701500635 7.367080499253574 4 2.5580188621190385 4.774583729146487 5 1.462466196210979 3.412146118359405 6 0.9017300138776949 2.524640562331666 7 0.6128397787773353 2.0017819412500906 8 0.4277278772954549 1.5967220521161336 9 0.3373790184601627 1.4168776825249907 >10 1.6941487260393926 14.32515881056119 >50 0.12962995273024133 4.189270719602707 >100 0.061331275742991194 5.051495991536333 >500 0.003733279561702219 1.1725875507074182 >1k 0.002750837571780578 2.7590511045341763 >5k 1.96488397984327E-4 0.7594908887547803 >10k+ 1.96488397984327E-4 1.5175147823722301 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 18560 0.8490731332857255 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 14542 0.6652597793233308 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 9878 0.45189355660540925 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 6689 0.30600485929677895 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 4472 0.20458270754600025 No Hit GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 4111 0.18806786912379403 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3585 0.164004697350718 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 3368 0.15407749530745277 No Hit GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA 3319 0.15183586903961868 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 3171 0.1450652427612627 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3132 0.14328109124196617 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 2925 0.13381136394723853 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2848 0.13028880838349924 No Hit GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT 2492 0.11400270733556185 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 2.287373742687838E-5 1.8298989941502704E-4 14-15 0.0 0.0 0.0 4.574747485375676E-5 3.4310606140317565E-4 16-17 0.0 0.0 0.0 9.149494970751352E-5 4.117272736838108E-4 18-19 0.0 0.0 0.0 2.058636368419054E-4 4.117272736838108E-4 20-21 0.0 0.0 0.0 2.973585865494189E-4 4.117272736838108E-4 22-23 0.0 0.0 0.0 5.260959608182027E-4 4.574747485375676E-4 24-25 0.0 0.0 0.0 9.378232345020136E-4 5.032222233913243E-4 26-27 0.0 0.0 0.0 0.0013724242456127026 5.032222233913243E-4 28-29 0.0 0.0 0.0 0.0038199141502886897 5.032222233913243E-4 30-31 0.0 0.0 0.0 0.009835707093557702 5.489696982450812E-4 32-33 0.0 0.0 0.0 0.019442676812846622 6.404646479525947E-4 34-35 0.0 0.0 0.0 0.03531705058710022 6.404646479525947E-4 36-37 0.0 0.0 0.0 0.060409540544385805 6.404646479525947E-4 38-39 0.0 0.0 0.0 0.09536061133265597 6.862121228063514E-4 40-41 0.0 0.0 0.0 0.13385711142209228 6.862121228063514E-4 42-43 0.0 0.0 0.0 0.1766767478852086 6.862121228063514E-4 44-45 0.0 0.0 0.0 0.23081888437462972 7.319595976601082E-4 46-47 0.0 0.0 0.0 0.29763307139854145 8.234545473676217E-4 48-49 0.0 0.0 0.0 0.3671463594388249 8.234545473676217E-4 50-51 0.0 0.0 0.0 0.44837100104167 8.234545473676217E-4 52-53 0.0 0.0 0.0 0.5288636830468549 8.692020222213784E-4 54-55 0.0 0.0 0.0 0.6196037994192816 9.149494970751351E-4 56-57 0.0 0.0 0.0 0.7305871734144954 0.0010064444467826486 58-59 0.0 0.0 0.0 0.8608531080605678 0.0010979393964901621 60-61 0.0 0.0 0.0 1.0052092649615973 0.0010979393964901621 62-63 0.0 0.0 0.0 1.1570222602637892 0.0010979393964901621 64-65 0.0 0.0 0.0 1.3149425434589574 0.0010979393964901621 66-67 0.0 0.0 0.0 1.496537144890945 0.0010979393964901621 68-69 0.0 0.0 0.0 1.7149584635802064 0.0010979393964901621 70-71 0.0 0.0 0.0 1.9637561055723625 0.0011208131339170407 72-73 0.0 0.0 0.0 2.2190727627311793 0.0013038030333320677 74-75 0.0 0.0 0.0 2.485460308804605 0.0013724242456127028 76-77 0.0 0.0 0.0 2.769048905423043 0.0014867929327470946 78-79 0.0 0.0 0.0 3.1090670122735897 0.0015325404076008515 80-81 0.0 0.0 0.0 3.475961760600719 0.0015554141450277298 82-83 0.0 0.0 0.0 3.862459301902683 0.0016926565695890002 84-85 0.0 0.0 0.0 4.267118590721589 0.0017155303070158785 86-87 0.0 0.0 0.0 4.698425783642808 0.0017384040444427568 88-89 0.0 0.0 0.0 5.166285208972178 0.0017384040444427568 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CTTTGCG 495 0.0 23.928226 2 TTTGCGT 440 0.0 23.688942 3 GAGTACT 10885 0.0 23.559536 12-13 GTACATG 11055 0.0 23.357801 1 TACATGG 11465 0.0 22.149626 2 AGTACTT 11325 0.0 21.619669 12-13 GTACTTT 11825 0.0 21.586607 14-15 ACATGGG 11475 0.0 21.139414 3 GTATCAA 25710 0.0 20.770275 1 TAGACCG 160 5.091876E-5 20.727825 5 GGTATCA 20160 0.0 19.636646 1 GTATAGG 655 0.0 19.56655 1 ACTTTTT 13495 0.0 19.197336 16-17 CATGGGA 6860 0.0 18.992592 4 TACTTTT 13025 0.0 18.67065 14-15 CATGGGG 4700 0.0 18.447134 4 CGGTTAC 235 0.0 18.17927 28-29 TATCAAC 29405 0.0 18.013426 2 CAACGCA 29550 0.0 17.909 5 ATCAACG 29580 0.0 17.87482 3 >>END_MODULE