FastQCFastQC Report
Thu 26 May 2016
SRR938881_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938881_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2185913
Sequences flagged as poor quality0
Sequence length101
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT181680.8311401231430529No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT159070.7277050824987088No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT110420.5051436173351821No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT80330.36748946550022804No Hit
GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA39390.18019930344894788No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT37880.17329143474603062No Hit
GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA35920.16432492967469428No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT34750.15897247511680476No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA33730.15430623268172156No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT31600.14456202053787137No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT31580.14447052558816384No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT25750.11779974774842365No Hit
GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT23100.10567666691217811No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACTATCG600.003898891631.7538531
TCGTATA753.094291E-431.6405472
GAGTACT129600.022.77930512-13
GTACATG112000.022.2418751
AGTACTT133450.021.53528612-13
GTACTTT137750.021.39710814-15
TACATGG115500.021.2032762
ACATGGG115950.020.2204783
GGTATCA243050.019.5775151
ACTTTTT160150.018.62595616-17
TACTTTT152900.018.62510914-15
CATGGGA71500.018.5860544
ATGGGAT27800.018.2672945
GTATCAA298400.018.2605931
CATGGGG44950.018.0551724
GTATAGG6900.017.947831
TGCACCG3751.7462298E-1017.7187065
TATAGCG1650.001472500917.2584785
AGAGTAC250900.016.27815810-11
ACCGTAT1750.002182576316.272288
Produced by FastQC (version 0.11.5)