FastQCFastQC Report
Thu 26 May 2016
SRR938880_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938880_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2173660
Sequences flagged as poor quality0
Sequence length101
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT185330.8526172446472768No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT142060.6535520734613509No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT98920.4550849718907281No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT67370.3099380767921386No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT44730.20578195301933144No Hit
GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA41410.19050817515158766No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA35910.16520522988875905No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT35420.16295096749261612No Hit
GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA33230.152875794742508No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT31780.14620501826412594No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT30300.1393962257206739No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT29850.13732598474462426No Hit
CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC29650.13640587764415776No Hit
GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT25420.11694561246929143No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACCGTAT1853.0195224E-1028.154498
GTACCGT2053.274181E-1127.71756
TACCGTA1501.0903441E-625.2537257
CCGTATG1751.5333535E-724.3518079
GAGTACT110450.023.12849812-13
GTACATG115850.023.1232721
TACATGG120600.021.987582
TTTGCGT5450.021.721023
TAGATCG1100.002930833721.5235545
GTACTTT118850.021.49383714-15
AGTACTT113750.021.41685512-13
ACATGGG120000.021.2688623
GTATCAA253800.020.9790121
GGTATCA198800.019.4373971
CATGGGG45100.019.4236974
TGTACCG3255.0931703E-1018.940735
CATGGGA73850.018.7227254
ACTTTTT137850.018.72153316-17
TACTTTT130750.018.57793214-15
TATCAAC290750.018.2241422
Produced by FastQC (version 0.11.5)