##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938880_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2173660 Sequences flagged as poor quality 0 Sequence length 101 %GC 43 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.33313351674135 33.0 31.0 34.0 31.0 34.0 2 32.43962947287064 34.0 31.0 34.0 31.0 34.0 3 32.470049593772714 34.0 31.0 34.0 31.0 34.0 4 36.00408711574027 37.0 35.0 37.0 35.0 37.0 5 36.03702280945502 37.0 35.0 37.0 35.0 37.0 6 36.12757560980098 37.0 37.0 37.0 35.0 37.0 7 36.07101524617465 37.0 37.0 37.0 35.0 37.0 8 36.063615744872706 37.0 37.0 37.0 35.0 37.0 9 37.76900987274919 39.0 38.0 39.0 35.0 39.0 10-11 37.68110008004932 39.0 38.0 39.0 35.0 39.0 12-13 37.57750568166134 39.0 38.0 39.0 35.0 39.0 14-15 38.88533625313987 40.0 38.0 41.0 36.0 41.0 16-17 38.82643444696963 40.0 38.0 41.0 36.0 41.0 18-19 38.81321940873917 40.0 38.0 41.0 35.5 41.0 20-21 38.72631874350174 40.0 38.0 41.0 35.0 41.0 22-23 38.79022202184334 40.0 38.0 41.0 35.0 41.0 24-25 38.72945791890176 40.0 38.0 41.0 35.0 41.0 26-27 38.64965035930182 40.0 38.0 41.0 35.0 41.0 28-29 38.525720213832884 40.0 38.0 41.0 35.0 41.0 30-31 38.32791742958881 40.0 38.0 41.0 34.5 41.0 32-33 38.11509872749188 40.0 38.0 41.0 34.0 41.0 34-35 37.814910105536285 40.0 37.5 41.0 33.5 41.0 36-37 37.55423249266215 40.0 37.0 41.0 33.0 41.0 38-39 37.358963453345964 40.0 37.0 41.0 33.0 41.0 40-41 37.20885810108297 40.0 37.0 41.0 33.0 41.0 42-43 36.98670445239826 40.0 36.0 41.0 32.0 41.0 44-45 36.87250765989161 40.0 37.0 41.0 31.5 41.0 46-47 36.56771735229981 40.0 36.0 41.0 31.0 41.0 48-49 36.32713993908891 40.0 36.0 41.0 30.0 41.0 50-51 35.16076502304868 38.5 34.5 40.0 28.0 40.5 52-53 35.312060303819365 38.5 35.0 39.5 28.5 40.5 54-55 35.80348467561624 39.0 35.0 40.5 29.0 41.0 56-57 35.70144732846903 39.0 35.0 41.0 28.5 41.0 58-59 35.50064476505065 39.0 35.0 41.0 28.0 41.0 60-61 35.23789254069173 38.5 35.0 40.0 28.0 41.0 62-63 34.894108324208936 38.0 35.0 40.0 27.5 41.0 64-65 34.49471168444007 37.5 34.5 40.0 26.0 41.0 66-67 34.11608462225003 37.0 34.0 39.5 26.0 41.0 68-69 33.72339993375229 36.0 34.0 39.0 25.5 41.0 70-71 33.300176890590066 36.0 34.0 39.0 25.0 40.5 72-73 32.871152802186174 35.0 33.0 38.5 23.5 40.0 74-75 32.42103318826311 35.0 33.0 37.0 22.5 39.5 76-77 32.01432514744717 35.0 32.5 37.0 21.0 39.0 78-79 31.59397214835807 35.0 32.0 36.0 19.5 39.0 80-81 31.17396119908357 35.0 32.0 36.0 18.0 37.5 82-83 30.819162610527865 35.0 31.0 35.5 15.5 37.0 84-85 30.49353808783342 34.5 31.0 35.0 9.5 37.0 86-87 30.133436231977402 34.0 31.0 35.0 6.0 36.0 88-89 29.74828538041828 34.0 30.5 35.0 2.0 36.0 90-91 29.358480627145 34.0 30.0 35.0 2.0 35.5 92-93 28.977924560418835 34.0 29.5 35.0 2.0 35.0 94-95 28.551045011639353 34.0 29.0 35.0 2.0 35.0 96-97 28.025171139920687 34.0 29.0 35.0 2.0 35.0 98-99 27.391149949854164 34.0 28.0 35.0 2.0 35.0 100-101 24.829645160696707 31.0 21.5 34.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.1271241294151153 1101 2 0.20424783730465634 1101 3 0.1539070709769632 1101 4 0.08470038754013132 1101 5 -0.01981777737949386 1101 6 0.03212264125765785 1101 7 0.02639946827414974 1101 8 0.06501002168740655 1101 9 0.06716706842631481 1101 10-11 0.13105639658426327 1101 12-13 0.176924098796178 1101 14-15 0.05657150248769227 1101 16-17 0.15356408344408834 1101 18-19 0.16854726969489775 1101 20-21 0.2318856023931204 1101 22-23 0.13953909416621002 1101 24-25 0.2515176373450032 1101 26-27 0.12202047531933147 1101 28-29 0.14053590981185238 1101 30-31 0.17865116423815408 1101 32-33 0.2937734760134205 1101 34-35 0.17281887685864916 1101 36-37 0.09438689101694564 1101 38-39 0.037730406591869325 1101 40-41 -0.037260980885143624 1101 42-43 -0.09087654193841388 1101 44-45 -0.007933716449812778 1101 46-47 0.11463918891194425 1101 48-49 -0.005981337115358087 1101 50-51 -0.04771274376751933 1101 52-53 -0.1385036214622133 1101 54-55 -0.11776016493951147 1101 56-57 -0.16631824954844632 1101 58-59 -0.13841268885622782 1101 60-61 -0.20062671932507214 1101 62-63 -0.1522501154649234 1101 64-65 -0.2036131896600608 1101 66-67 -0.27816412093301324 1101 68-69 -0.3008766693652092 1101 70-71 -0.2878362289723384 1101 72-73 -0.2636396920941664 1101 74-75 -0.35751447536071623 1101 76-77 -0.39756272808746473 1101 78-79 -0.5078045687243069 1101 80-81 -0.4392284732739036 1101 82-83 -0.6522715875196852 1101 84-85 -0.7173371624124982 1101 86-87 -0.7236206543395447 1101 88-89 -0.7892601328327977 1101 90-91 -0.8759271050149842 1101 92-93 -0.7021995029280852 1101 94-95 -0.780685785356976 1101 96-97 -0.922232531409275 1101 98-99 -0.84322654706029 1101 100-101 -0.6413801271678388 1102 1 0.17231575626841789 1102 2 0.06581652519338377 1102 3 0.11472037368822185 1102 4 0.10974524830202625 1102 5 0.12799587344050423 1102 6 0.1788186798233653 1102 7 0.2133176928980447 1102 8 0.13016843271652334 1102 9 0.25161361325851317 1102 10-11 0.14236227854414807 1102 12-13 0.1998546570311035 1102 14-15 0.26412869524401117 1102 16-17 0.2704728994325265 1102 18-19 0.33514876925679715 1102 20-21 0.298209261608676 1102 22-23 0.2736079464096832 1102 24-25 0.32630314093331947 1102 26-27 0.31388033514898694 1102 28-29 0.3068039578486932 1102 30-31 0.29031916550126624 1102 32-33 0.23482735095939944 1102 34-35 0.17551860559249377 1102 36-37 0.16513875199762396 1102 38-39 0.2042789893151138 1102 40-41 0.14067312848997204 1102 42-43 0.13585907109399642 1102 44-45 0.2802190547866985 1102 46-47 0.1412277987510251 1102 48-49 0.18540571992166122 1102 50-51 0.19175987688771556 1102 52-53 0.21912701900397025 1102 54-55 0.23096783712112057 1102 56-57 0.35821091452717013 1102 58-59 0.19359359218930905 1102 60-61 0.019204593432846195 1102 62-63 0.09790049814408519 1102 64-65 0.14677172376001124 1102 66-67 0.12888491928917034 1102 68-69 0.17721532833705567 1102 70-71 0.13278603425175106 1102 72-73 0.21190313659646165 1102 74-75 0.048252813411103546 1102 76-77 -0.07228119649525055 1102 78-79 -0.21655093540674386 1102 80-81 -0.24999764269508518 1102 82-83 -0.2594622422550934 1102 84-85 -0.2309707386316049 1102 86-87 -0.2262470970239292 1102 88-89 -0.31294521405637354 1102 90-91 -0.38299457452313845 1102 92-93 -0.5164130089003578 1102 94-95 -0.43525727212615095 1102 96-97 -0.4997928819321409 1102 98-99 -0.6667107382894599 1102 100-101 -0.4817651591388774 1103 1 -0.032918802133650615 1103 2 0.046165797661011254 1103 3 0.15701653545198013 1103 4 0.08240148650876478 1103 5 0.12780222134748698 1103 6 0.1334178307732259 1103 7 0.058780303595668215 1103 8 0.04398958530445185 1103 9 0.1865894239987469 1103 10-11 0.16916659633887576 1103 12-13 0.22765871992964293 1103 14-15 0.19853961549686971 1103 16-17 0.1622265549931612 1103 18-19 0.15846581827492656 1103 20-21 0.3248895816823776 1103 22-23 0.2847774454718319 1103 24-25 0.2632326166364578 1103 26-27 0.13199789016407948 1103 28-29 0.0752544952656038 1103 30-31 0.16062035273092334 1103 32-33 0.14776758060748563 1103 34-35 0.025210401170070895 1103 36-37 0.038231613136133547 1103 38-39 0.10072169956457344 1103 40-41 0.04390422875733435 1103 42-43 -0.032100047180854574 1103 44-45 0.055510816033077504 1103 46-47 0.01305361882882039 1103 48-49 0.08087822887213747 1103 50-51 -0.21517530486705994 1103 52-53 -0.13208660599500632 1103 54-55 -0.02581257391299374 1103 56-57 0.02700782609975505 1103 58-59 -0.013350409577277844 1103 60-61 0.026199682260504176 1103 62-63 -0.08628895139661097 1103 64-65 -0.17839968422009633 1103 66-67 -0.29838211487079747 1103 68-69 -0.25990337867077784 1103 70-71 -0.29977343828998215 1103 72-73 -0.21976797314216867 1103 74-75 -0.20776150735525079 1103 76-77 -0.34736464157029445 1103 78-79 -0.3315713292580078 1103 80-81 -0.40139735053488934 1103 82-83 -0.31058035666164585 1103 84-85 -0.42550041204101774 1103 86-87 -0.30379246832917417 1103 88-89 -0.40712665873548914 1103 90-91 -0.4448135844304488 1103 92-93 -0.3920852382844977 1103 94-95 -0.3815356368743963 1103 96-97 -0.43318874605823865 1103 98-99 -0.49667039913520483 1103 100-101 -0.15699625854364285 1104 1 0.07317981068556634 1104 2 0.09656188081189043 1104 3 0.12402952497573949 1104 4 0.13213981107417538 1104 5 0.11053485017172449 1104 6 0.11291395342477273 1104 7 0.07009445428082017 1104 8 0.07639529009695423 1104 9 0.09937270067344883 1104 10-11 0.06713437715280435 1104 12-13 0.16990832898729735 1104 14-15 0.13131126166405238 1104 16-17 0.12776540954415339 1104 18-19 0.13337256990097757 1104 20-21 0.05229862799394169 1104 22-23 0.026495157631927668 1104 24-25 0.06703546745092126 1104 26-27 0.11174556043764738 1104 28-29 0.037806853420207176 1104 30-31 0.13319616962358083 1104 32-33 0.10007766654670291 1104 34-35 0.12734631509641048 1104 36-37 0.07391303916728731 1104 38-39 0.14619118409186882 1104 40-41 0.0660738127173417 1104 42-43 0.11463591920799132 1104 44-45 0.2943547339191497 1104 46-47 0.47067893897535384 1104 48-49 0.5714760598066562 1104 50-51 0.47679254888468847 1104 52-53 0.42309404474222134 1104 54-55 0.339545188319363 1104 56-57 0.3953862468727962 1104 58-59 0.24192758679428294 1104 60-61 0.32309343162064863 1104 62-63 0.28681329205667083 1104 64-65 0.2547136932362264 1104 66-67 0.21654267788252213 1104 68-69 0.27899862584521884 1104 70-71 0.299858093874505 1104 72-73 0.21678435689182152 1104 74-75 0.24592934435204228 1104 76-77 0.15787348065856577 1104 78-79 0.1083058232316958 1104 80-81 0.058410172694269846 1104 82-83 0.11769473845411227 1104 84-85 0.11438306758538275 1104 86-87 0.09111291241726249 1104 88-89 0.2057917506399214 1104 90-91 0.2862822273126291 1104 92-93 0.26876518600713695 1104 94-95 0.2690436256714577 1104 96-97 0.17703557730062514 1104 98-99 0.3080166031245497 1104 100-101 0.8203406188650071 1105 1 0.09649843756966447 1105 2 0.10253770831077702 1105 3 0.07687671637557969 1105 4 0.09207103597345423 1105 5 0.1648927205986226 1105 6 0.142418807586715 1105 7 0.11908228490404582 1105 8 0.15505121237695363 1105 9 0.19321810873595524 1105 10-11 0.14472613309939675 1105 12-13 0.23262339923218178 1105 14-15 0.18733951682536798 1105 16-17 0.19127653176747828 1105 18-19 0.19798242259744825 1105 20-21 0.2675949934185198 1105 22-23 0.11959286044105966 1105 24-25 0.20214227494976456 1105 26-27 0.20182950714986703 1105 28-29 0.22094134065742566 1105 30-31 0.1940694342291991 1105 32-33 0.17122703294618447 1105 34-35 0.2650799972152882 1105 36-37 0.365441207003002 1105 38-39 0.39864744538039787 1105 40-41 0.30808793512952803 1105 42-43 0.2143509857918744 1105 44-45 0.32628467703798947 1105 46-47 0.41753835079060053 1105 48-49 0.21241591583462593 1105 50-51 0.2177141860576981 1105 52-53 0.31319860072654393 1105 54-55 0.24600708883880884 1105 56-57 0.16329612427023932 1105 58-59 0.14887166244688643 1105 60-61 0.31223652306745464 1105 62-63 0.23063127492793 1105 64-65 0.32011852114665373 1105 66-67 0.11806847468285042 1105 68-69 0.156471011681802 1105 70-71 0.12599390273244637 1105 72-73 0.24364812314262707 1105 74-75 0.17874688596521793 1105 76-77 0.25486993228889787 1105 78-79 0.07881335186124616 1105 80-81 -0.024542147528595848 1105 82-83 -0.11878589130666839 1105 84-85 0.12292730139316177 1105 86-87 0.20179934761832996 1105 88-89 0.04199433827724519 1105 90-91 -0.01894163094554102 1105 92-93 0.025902788539340804 1105 94-95 -0.08685459941150953 1105 96-97 -0.09082067722582465 1105 98-99 -0.001074239402740318 1105 100-101 0.18918607538191878 1106 1 0.0246395377991675 1106 2 0.07363138094148525 1106 3 0.08806093811560345 1106 4 0.09201127114481267 1106 5 0.07559155268167927 1106 6 0.12323753395722292 1106 7 0.07741131207718865 1106 8 0.06259308066001523 1106 9 0.09961603073228531 1106 10-11 0.07185630154344125 1106 12-13 0.10252583153124561 1106 14-15 0.10061082562980062 1106 16-17 0.19133500205108334 1106 18-19 0.15465205880656185 1106 20-21 0.2306281814535538 1106 22-23 0.14124498900750382 1106 24-25 0.15992468048627018 1106 26-27 0.21560939954296998 1106 28-29 0.1499942320992531 1106 30-31 0.14559972669065502 1106 32-33 0.2113594390112965 1106 34-35 0.19443516492035684 1106 36-37 0.10800258947352148 1106 38-39 0.2028504231360131 1106 40-41 0.24937093281250355 1106 42-43 0.29337627106105657 1106 44-45 0.2024203436305072 1106 46-47 0.12840074863751738 1106 48-49 0.15895519783249057 1106 50-51 0.2741798666981907 1106 52-53 0.1398728304129193 1106 54-55 0.12732999734490136 1106 56-57 0.2131308216584742 1106 58-59 0.44768286017504977 1106 60-61 0.4476989011078274 1106 62-63 0.30597757111594603 1106 64-65 0.14694281017712996 1106 66-67 0.27356382910014787 1106 68-69 0.20058975339266283 1106 70-71 0.18475934260913363 1106 72-73 0.1385366768402463 1106 74-75 0.10088712273319089 1106 76-77 0.05440359401953998 1106 78-79 0.14836752997295122 1106 80-81 0.08655344102875162 1106 82-83 0.13167135278389708 1106 84-85 -0.023735588078803715 1106 86-87 0.13240569308967665 1106 88-89 0.2094890462850607 1106 90-91 0.25111729027125307 1106 92-93 0.23941770914166227 1106 94-95 0.3138273669074465 1106 96-97 0.2690387826511582 1106 98-99 0.2205615778963974 1106 100-101 0.7590665867185713 1107 1 0.09588561212961366 1107 2 0.005006637214343357 1107 3 0.12653294366326406 1107 4 0.026472012673494305 1107 5 0.007309098038312811 1107 6 0.04115607892228468 1107 7 0.04148139121655703 1107 8 0.12961559182770088 1107 9 0.09982246967386033 1107 10-11 0.1065838820524121 1107 12-13 0.14388343172517182 1107 14-15 0.026675051670231653 1107 16-17 0.1342959224935143 1107 18-19 0.2152095904538669 1107 20-21 0.19381345884977463 1107 22-23 0.1356800166792027 1107 24-25 0.12675182938247076 1107 26-27 0.25389394521303643 1107 28-29 0.1646851375093732 1107 30-31 0.2769133176538645 1107 32-33 0.1848627788826036 1107 34-35 0.08093170518127835 1107 36-37 0.09350225162398118 1107 38-39 0.18212783800547783 1107 40-41 0.15433854187664053 1107 42-43 0.1513974450371407 1107 44-45 0.14396704633445268 1107 46-47 0.29900188995628696 1107 48-49 0.23754794574346505 1107 50-51 0.22996353111814472 1107 52-53 0.17136608984851165 1107 54-55 0.3135285995978876 1107 56-57 0.39492894479553087 1107 58-59 0.3078421200492727 1107 60-61 0.19464893222905744 1107 62-63 0.2128072372353671 1107 64-65 0.24194767592078392 1107 66-67 0.1621672674406156 1107 68-69 0.20919093200363648 1107 70-71 0.18805881403665126 1107 72-73 0.04062435168070522 1107 74-75 0.0713058652580969 1107 76-77 0.13024049304914342 1107 78-79 0.05791042532128188 1107 80-81 0.004250119625961446 1107 82-83 0.04844327371378654 1107 84-85 0.042971382276565606 1107 86-87 -0.03087319215779516 1107 88-89 0.08573223047033096 1107 90-91 0.018415430722544812 1107 92-93 -0.12072834307984692 1107 94-95 -0.03543913111562347 1107 96-97 0.15941648600239233 1107 98-99 0.0317571834550634 1107 100-101 0.06269753693260682 1108 1 0.1284008284822633 1108 2 0.08261522900063056 1108 3 0.017115423565215337 1108 4 0.12433445269094534 1108 5 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-1.3163219974476235 2316 88-89 -1.3022536546358978 2316 90-91 -1.6251236825416093 2316 92-93 -1.7227938267749003 2316 94-95 -1.8732422603039431 2316 96-97 -1.962822699283013 2316 98-99 -2.466165647282267 2316 100-101 -3.044688655973065 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 6187.0 3 1133.0 4 996.0 5 1074.0 6 1173.0 7 1469.0 8 1747.0 9 2056.0 10 2769.0 11 3678.0 12 5592.0 13 10506.0 14 10736.0 15 9844.0 16 14294.0 17 22218.0 18 18551.0 19 10279.0 20 9486.0 21 9598.0 22 9917.0 23 10682.0 24 11905.0 25 13674.0 26 16116.0 27 19267.0 28 23390.0 29 28915.0 30 36498.0 31 47433.0 32 63216.0 33 87341.0 34 128297.0 35 203860.0 36 313901.0 37 489722.0 38 452004.0 39 74122.0 40 14.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.61597721335911 19.333547941970807 15.123452235127438 21.927022609542643 2 14.988837389611245 20.99625089146646 40.76583472338412 23.24907699553818 3 18.9157233376214 25.92091414132578 30.852822252037136 24.310540269015682 4 12.363173873422104 17.224884568964605 37.895639384558145 32.516302173055145 5 12.992694343584585 38.00051114268018 34.1477847030192 14.859009810716037 6 30.04650764507973 36.56899539435279 17.845179539214296 15.539317421353187 7 26.95982397117385 34.32396357687701 21.21098550266884 17.50522694928029 8 25.75377595863653 34.23927181147025 20.119845393457076 19.887106836436132 9 26.53716542096653 17.2155712747212 21.162306601082662 35.084956703229615 10-11 24.488872293924846 26.77456513802186 27.23872024589194 21.49784232216135 12-13 26.334364980119084 24.752202186808795 27.80013370584346 21.11329912722866 14-15 23.07888886637775 25.470216837556936 27.404819308175288 24.04607498789003 16-17 20.96507788592998 29.017783457156003 27.865384309922163 22.15175434699185 18-19 20.89354724784519 27.337527759602647 30.79495950539905 20.973965487153112 20-21 22.11866939321675 26.113201865240214 30.59110451524613 21.177024226296904 22-23 21.72003515557632 26.204075035096864 30.637458464589184 21.438431344737634 24-25 21.706331240682207 26.357755374901725 30.69426859839659 21.241644786019474 26-27 21.483605245492406 26.25143498000527 31.033266204479325 21.231693570022994 28-29 20.9187725536169 26.778688960595314 31.111769686511202 21.19076879927658 30-31 21.092843001764233 26.534584889681383 30.892574337445883 21.4799977711085 32-33 21.00514645002703 26.700432953857856 30.78717752950137 21.50724306661374 34-35 21.662058045544562 26.50881946977006 30.515388485987177 21.3137339986982 36-37 21.763725159207684 26.19196492574933 30.73524582961425 21.309064085428737 38-39 21.4614862988694 26.502747717056234 30.79896153427323 21.23680444980113 40-41 21.384050303720315 26.674853085901006 30.752854730580832 21.18824187979785 42-43 21.197518549042407 26.919234943748922 30.122650811025743 21.760595696182925 44-45 21.279081678308547 26.90468994919717 29.94964574904636 21.86658262344792 46-47 21.74762965102017 27.04390795631284 29.556805026903916 21.65165736576307 48-49 21.75868905957109 27.134553988048133 29.342240422019884 21.764516530360893 50-51 21.48266931810299 27.46978981201008 29.44689805073184 21.600642819155087 52-53 21.677365677714384 27.694487024409288 29.006070699262327 21.622076598614004 54-55 21.194349203222302 27.367575780066648 29.234542656005814 22.203532360705232 56-57 21.680957503810607 27.74767098728133 28.70819369581991 21.863177813088146 58-59 21.92551007176335 27.27881056874657 28.904864037493443 21.890815321996634 60-61 21.69963258592922 27.21883743931592 29.505408400735707 21.57612157401916 62-63 21.644674754437645 27.31118528831469 29.448616976365997 21.59552298088167 64-65 21.458943199799076 27.474205799491862 29.350439890158125 21.716411110550933 66-67 21.40684142416529 27.566806175068205 28.90765962417154 22.11869277659497 68-69 21.152803516527438 27.64785041990528 29.21438808185123 21.984957981716054 70-71 21.536964175064767 27.499753093387135 28.931880836276747 22.03140189527135 72-73 21.4334017440809 27.39885131000337 28.96367199659935 22.20407494931638 74-75 21.230656870834892 28.10655649747827 28.61814109846032 22.04464553322652 76-77 21.463361282592118 27.49648418333394 28.76166948224888 22.278485051825054 78-79 21.43264348003793 27.544089286845413 28.85263130153267 22.170635931583988 80-81 21.51815776607291 27.862273988721252 28.60372621701937 22.015842028186466 82-83 21.462587613392238 27.664399669660867 28.813118865675342 22.059893851271557 84-85 21.36763539588107 27.64390472865331 28.54901026842177 22.439449607043844 86-87 21.43305835223583 27.589302579470814 28.802060080915236 22.17557898737812 88-89 21.58075887432524 27.416754245097053 28.555493508790793 22.44699337178691 90-91 21.41010541009246 27.306026443523468 28.790384442385214 22.49348370399886 92-93 21.549747122225956 27.555628947312428 28.66219470339708 22.232429227064536 94-95 21.506243752662563 27.226642416628543 28.545469515306067 22.721644315402827 96-97 21.20930325065206 27.194464192133477 28.653786291100314 22.942446266114153 98-99 21.328619897729087 27.39142099213425 28.33954313441155 22.940415975725113 100-101 21.50546187417133 26.769033548176452 28.895397744956536 22.83010683269568 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 6698.0 1 4178.5 2 1877.0 3 2046.0 4 1696.0 5 1712.5 6 3052.5 7 6312.5 8 9968.0 9 10711.5 10 8098.0 11 5668.0 12 5185.0 13 5310.5 14 5523.5 15 5554.0 16 5640.0 17 5724.0 18 5720.0 19 5929.5 20 6264.0 21 6916.5 22 7863.0 23 8860.0 24 9939.5 25 11704.5 26 14407.5 27 16961.5 28 18992.5 29 21620.5 30 24968.5 31 28365.5 32 31825.0 33 36479.5 34 41721.0 35 45145.5 36 49748.0 37 55042.0 38 57466.5 39 58506.5 40 59166.0 41 61029.0 42 63249.5 43 66228.5 44 70915.5 45 73506.0 46 73422.0 47 79651.5 48 102247.0 49 102587.0 50 81045.5 51 72806.0 52 68896.5 53 67969.5 54 66296.0 55 61118.5 56 55378.5 57 50021.0 58 46309.5 59 42066.5 60 35982.5 61 29278.5 62 23172.5 63 18164.0 64 13480.5 65 9780.5 66 6897.5 67 5148.0 68 3736.5 69 2408.5 70 1655.5 71 1128.5 72 822.5 73 568.0 74 417.0 75 330.5 76 226.5 77 134.0 78 81.5 79 51.0 80 40.5 81 35.0 82 28.5 83 21.0 84 17.0 85 22.5 86 19.0 87 11.0 88 10.0 89 6.5 90 5.5 91 5.5 92 3.0 93 2.0 94 2.0 95 1.0 96 1.0 97 1.5 98 0.5 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.2471407671853004 2 0.07715098037411554 3 0.08014132845063165 4 0.09279280108204595 5 0.0945410045729323 6 0.07103226815601336 7 0.08018733380565499 8 0.07797907676453539 9 0.08230358013672791 10-11 0.07586283043346245 12-13 0.0794972534803051 14-15 0.08722615312422366 16-17 0.0768059402114406 18-19 0.08690411563906039 20-21 0.08874432983999338 22-23 0.09980861772310298 24-25 0.11091891096123589 26-27 0.11328818674493711 28-29 0.10611135136129847 30-31 0.10015365788577792 32-33 0.09504706347818886 34-35 0.34181978782330263 36-37 0.5240009937156686 38-39 0.5081981542651565 40-41 0.47482126919573436 42-43 0.49743290118969846 44-45 0.5189634073406144 46-47 0.5086582078153897 48-49 0.528463513152931 50-51 0.5203205653138026 52-53 0.5239319856831335 54-55 0.5138108075780021 56-57 0.48853086499268517 58-59 0.48344727326260784 60-61 0.43615376829863 62-63 0.5047247499608954 64-65 0.5356403485365696 66-67 0.5475787381651224 68-69 0.5512361638894767 70-71 0.5479927863603323 72-73 0.5391367555183424 74-75 0.5360543967317796 76-77 0.5193544528583127 78-79 0.49837601096767664 80-81 0.4942125263380658 82-83 0.5076230873273648 84-85 0.5154900030363534 86-87 0.5072780471646899 88-89 0.5204355787013609 90-91 0.5327650138476119 92-93 0.5403789001039722 94-95 0.5400568626188088 96-97 0.5365604556370361 98-99 0.540792948299182 100-101 0.5201825492487325 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2173660.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 46.58274561434351 #Duplication Level Percentage of deduplicated Percentage of total 1 72.06597411774716 33.570309397768796 2 14.403165919396384 13.418780281288475 3 5.31723504636064 7.4307422260886895 4 2.5388505539391395 4.730665180279281 5 1.4526823528596624 3.3834966250853826 6 0.9137179567531305 2.5538094685613277 7 0.6166759651823139 2.0108521728797237 8 0.4556761281057341 1.6981316126462733 9 0.3263476746592542 1.3681953639436117 >10 1.7129341298052405 14.463988506460016 >50 0.12701738406642524 4.09513098669588 >100 0.06318897954424761 5.168322244152063 >500 0.003365892632451779 1.0656818678678233 >1k 0.002771911579666166 2.7652666720682877 >5k 1.9799368426186897E-4 0.7668537704259816 >10k+ 1.9799368426186897E-4 1.5097736237883341 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 18533 0.8526172446472768 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 14206 0.6535520734613509 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 9892 0.4550849718907281 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 6737 0.3099380767921386 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 4473 0.20578195301933144 No Hit GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 4141 0.19050817515158766 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 3591 0.16520522988875905 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3542 0.16295096749261612 No Hit GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA 3323 0.152875794742508 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 3178 0.14620501826412594 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3030 0.1393962257206739 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2985 0.13732598474462426 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 2965 0.13640587764415776 No Hit GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT 2542 0.11694561246929143 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 4.6005355023324714E-5 2 0.0 0.0 0.0 0.0 4.6005355023324714E-5 3 0.0 0.0 0.0 0.0 4.6005355023324714E-5 4 0.0 0.0 0.0 0.0 4.6005355023324714E-5 5 0.0 0.0 0.0 0.0 9.201071004664943E-5 6 0.0 0.0 0.0 0.0 9.201071004664943E-5 7 0.0 0.0 0.0 0.0 9.201071004664943E-5 8 0.0 0.0 0.0 0.0 1.3801606506997415E-4 9 0.0 0.0 0.0 0.0 1.3801606506997415E-4 10-11 0.0 0.0 0.0 9.201071004664943E-5 1.8402142009329886E-4 12-13 0.0 0.0 0.0 9.201071004664943E-5 2.760321301399483E-4 14-15 0.0 0.0 0.0 1.150133875583118E-4 3.680428401865977E-4 16-17 0.0 0.0 0.0 1.610187425816365E-4 4.140481952099224E-4 18-19 0.0 0.0 0.0 2.070240976049612E-4 4.6005355023324713E-4 20-21 0.0 0.0 0.0 3.450401626749354E-4 5.520642602798966E-4 22-23 0.0 0.0 0.0 5.290615827682342E-4 5.75066937791559E-4 24-25 0.0 0.0 0.0 8.050937129081826E-4 6.44074970326546E-4 26-27 0.0 0.0 0.0 0.0013111526181647545 6.44074970326546E-4 28-29 0.0 0.0 0.0 0.003427398949237691 6.44074970326546E-4 30-31 0.0 0.0 0.0 0.00848798800180341 6.44074970326546E-4 32-33 0.0 0.0 0.0 0.018517155396888196 6.670776478382084E-4 34-35 0.0 0.0 0.0 0.034941067140215123 6.900803253498708E-4 36-37 0.0 0.0 4.6005355023324714E-5 0.06153216234369681 6.900803253498708E-4 38-39 0.0 0.0 4.6005355023324714E-5 0.0990495293652181 7.820910353965202E-4 40-41 0.0 0.0 4.6005355023324714E-5 0.14038534085367538 7.820910353965202E-4 42-43 0.0 0.0 4.6005355023324714E-5 0.1857696235841852 7.820910353965202E-4 44-45 0.0 0.0 4.6005355023324714E-5 0.24072302015954658 7.820910353965202E-4 46-47 0.0 0.0 4.6005355023324714E-5 0.3097540553720453 7.820910353965202E-4 48-49 0.0 0.0 4.6005355023324714E-5 0.38706605448874254 7.820910353965202E-4 50-51 0.0 0.0 4.6005355023324714E-5 0.469599661400587 7.820910353965202E-4 52-53 0.0 0.0 4.6005355023324714E-5 0.5556986833267392 8.050937129081826E-4 54-55 0.0 0.0 4.6005355023324714E-5 0.6509757735800448 8.280963904198448E-4 56-57 0.0 0.0 4.6005355023324714E-5 0.768059402114406 8.280963904198448E-4 58-59 0.0 0.0 4.6005355023324714E-5 0.8998647442562314 8.280963904198448E-4 60-61 0.0 0.0 4.6005355023324714E-5 1.0520504586733894 8.280963904198448E-4 62-63 0.0 0.0 4.6005355023324714E-5 1.2071345104570173 8.741017454431696E-4 64-65 0.0 0.0 4.6005355023324714E-5 1.3660370067075807 8.741017454431696E-4 66-67 0.0 0.0 4.6005355023324714E-5 1.5553950479835854 8.971044229548319E-4 68-69 0.0 0.0 4.6005355023324714E-5 1.7790960867845018 9.431097779781566E-4 70-71 0.0 0.0 4.6005355023324714E-5 2.022096372017703 9.66112455489819E-4 72-73 0.0 0.0 4.6005355023324714E-5 2.2791742958880414 0.0010811258430481307 74-75 0.0 0.0 4.6005355023324714E-5 2.553251198439498 0.0012421445856297673 76-77 0.0 0.0 4.6005355023324714E-5 2.8463283126155883 0.001288149940653092 78-79 0.0 0.0 4.6005355023324714E-5 3.1871359826283783 0.0013801606506997416 80-81 0.0 0.0 4.6005355023324714E-5 3.571510723848256 0.001426166005723066 82-83 0.0 0.0 4.6005355023324714E-5 3.961567126413515 0.001610187425816365 84-85 0.0 0.0 4.6005355023324714E-5 4.370623740603406 0.001610187425816365 86-87 0.0 4.6005355023324714E-5 4.6005355023324714E-5 4.802614024272425 0.0016331901033280272 88-89 0.0 4.6005355023324714E-5 4.6005355023324714E-5 5.270810522344801 0.0016561927808396897 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position ACCGTAT 185 3.0195224E-10 28.15449 8 GTACCGT 205 3.274181E-11 27.7175 6 TACCGTA 150 1.0903441E-6 25.253725 7 CCGTATG 175 1.5333535E-7 24.351807 9 GAGTACT 11045 0.0 23.128498 12-13 GTACATG 11585 0.0 23.123272 1 TACATGG 12060 0.0 21.98758 2 TTTGCGT 545 0.0 21.72102 3 TAGATCG 110 0.0029308337 21.523554 5 GTACTTT 11885 0.0 21.493837 14-15 AGTACTT 11375 0.0 21.416855 12-13 ACATGGG 12000 0.0 21.268862 3 GTATCAA 25380 0.0 20.979012 1 GGTATCA 19880 0.0 19.437397 1 CATGGGG 4510 0.0 19.423697 4 TGTACCG 325 5.0931703E-10 18.94073 5 CATGGGA 7385 0.0 18.722725 4 ACTTTTT 13785 0.0 18.721533 16-17 TACTTTT 13075 0.0 18.577932 14-15 TATCAAC 29075 0.0 18.224142 2 >>END_MODULE