FastQCFastQC Report
Thu 26 May 2016
SRR938880_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938880_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2173660
Sequences flagged as poor quality0
Sequence length101
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT183970.8463605163641048No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT159630.7343834822373324No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT110480.5082671622976914No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT82700.3804642860428954No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT39030.17955890065603636No Hit
GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA38910.17900683639575649No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA35960.16543525666387568No Hit
GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA35430.16299697284763948No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT34990.16097273722661318No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT32310.14864330208036214No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT31860.14657306110431254No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT24120.11096491631625921No Hit
GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT23910.10999880386076939No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGTACT131700.023.56975412-13
GTACTTT139750.022.33093814-15
AGTACTT134150.022.16631312-13
GTACATG105950.021.6131441
CTTTGCG5200.020.99172
TTTGCGT5050.020.6754233
TACATGG110350.020.4288712
GTATCAA290900.020.0805071
GGTATCA231200.019.7471581
ACTTTTT159550.019.5283716-17
GTATAGG7100.019.445251
ACATGGG111050.019.103463
TACTTTT154500.019.09306714-15
AGAGTAC243350.017.62218910-11
TATAACG1350.00944932117.577592
TATCAAC333700.017.4221972
ATCAACG336900.017.2285423
CAACGCA338150.017.178895
TCAACGC338450.017.1636644
AACGCAG342550.016.9720846
Produced by FastQC (version 0.11.5)