##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938879_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1046172 Sequences flagged as poor quality 0 Sequence length 101 %GC 40 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.186470293603726 33.0 31.0 34.0 31.0 34.0 2 32.2628563945508 34.0 31.0 34.0 31.0 34.0 3 32.2774065832387 34.0 31.0 34.0 30.0 34.0 4 35.83174564029624 37.0 35.0 37.0 35.0 37.0 5 35.8823214538336 37.0 35.0 37.0 35.0 37.0 6 36.001338212072206 37.0 36.0 37.0 35.0 37.0 7 35.956004366394815 37.0 36.0 37.0 35.0 37.0 8 35.96085251755925 37.0 36.0 37.0 35.0 37.0 9 37.61058219872067 39.0 38.0 39.0 35.0 39.0 10-11 37.53455598123445 39.0 37.0 39.0 35.0 39.0 12-13 37.44501047628879 39.0 37.0 39.0 35.0 39.0 14-15 38.63173789778354 40.0 38.0 41.0 34.5 41.0 16-17 38.57013760643565 40.0 38.0 41.0 34.5 41.0 18-19 38.56038729769101 40.0 38.0 41.0 35.0 41.0 20-21 38.67703255296452 40.0 38.0 41.0 35.0 41.0 22-23 38.6167996275947 40.0 38.0 41.0 35.0 41.0 24-25 38.53204539980042 40.0 38.0 41.0 34.5 41.0 26-27 38.37580292724332 40.0 38.0 41.0 34.0 41.0 28-29 38.238405826193016 40.0 38.0 41.0 34.0 41.0 30-31 37.99644561314965 40.0 37.5 41.0 34.0 41.0 32-33 37.71300082586802 40.0 36.5 41.0 33.5 41.0 34-35 37.384099842090976 40.0 36.0 41.0 33.0 41.0 36-37 37.0938750989321 40.0 35.0 41.0 33.0 41.0 38-39 36.83685808834494 39.0 35.0 41.0 31.5 41.0 40-41 36.69175049609433 39.0 35.0 41.0 31.5 41.0 42-43 36.46274752144007 39.0 35.0 41.0 31.0 41.0 44-45 36.25340097039492 39.0 35.0 41.0 30.5 41.0 46-47 35.876196743938856 39.0 35.0 41.0 29.0 41.0 48-49 35.53500189261422 39.0 35.0 41.0 27.0 41.0 50-51 34.39415793961223 38.0 34.0 40.0 25.0 40.5 52-53 34.510749666402845 38.0 34.5 39.5 25.0 40.5 54-55 35.01849170117342 39.0 35.0 40.5 26.0 41.0 56-57 34.94216964323266 39.0 35.0 41.0 26.0 41.0 58-59 34.75609077666005 38.5 35.0 40.5 25.5 41.0 60-61 34.43273620398941 38.0 35.0 40.0 24.5 41.0 62-63 34.075741369488 37.5 34.0 40.0 22.5 41.0 64-65 33.67600882072929 37.0 34.0 40.0 22.0 41.0 66-67 33.25420055210807 36.5 33.5 39.0 20.5 41.0 68-69 32.823501775998594 36.0 33.0 39.0 19.0 41.0 70-71 32.33725859610083 35.0 33.0 38.5 15.0 40.0 72-73 31.875345067541474 35.0 32.0 37.5 10.0 40.0 74-75 31.41900041293401 35.0 32.0 37.0 7.0 39.0 76-77 30.99338923236332 35.0 31.0 36.5 2.0 39.0 78-79 30.60890465430159 35.0 31.0 36.0 2.0 38.5 80-81 30.176561311141953 35.0 31.0 36.0 2.0 37.5 82-83 29.718405768841073 34.5 30.5 35.0 2.0 37.0 84-85 29.30758756686281 34.0 30.0 35.0 2.0 36.5 86-87 28.90412953128166 34.0 29.5 35.0 2.0 36.0 88-89 28.412014945917115 34.0 29.0 35.0 2.0 36.0 90-91 27.942284347124563 34.0 28.5 35.0 2.0 35.0 92-93 27.487177538683888 34.0 27.0 35.0 2.0 35.0 94-95 26.98219843390953 34.0 26.0 35.0 2.0 35.0 96-97 26.35767110953075 33.0 25.0 35.0 2.0 35.0 98-99 25.606804617213996 33.0 22.0 35.0 2.0 35.0 100-101 22.585250800059647 29.0 2.0 33.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.10498588845026546 1101 2 0.25326848745619657 1101 3 0.2738137870633466 1101 4 0.14968346356200612 1101 5 0.029277874420081673 1101 6 0.12428362757962219 1101 7 0.15336384019215643 1101 8 0.1544158959222628 1101 9 0.18865898258783886 1101 10-11 0.13238124620848168 1101 12-13 0.2247147310997235 1101 14-15 0.24430567970686212 1101 16-17 0.15393744477975702 1101 18-19 0.2415894068087141 1101 20-21 0.21351204754230224 1101 22-23 0.3333710722980783 1101 24-25 0.3323441210663063 1101 26-27 0.2665659629681798 1101 28-29 0.14340424667077656 1101 30-31 0.13357282509367963 1101 32-33 -0.06007782908758941 1101 34-35 -0.13528724268243053 1101 36-37 -0.22966880994304262 1101 38-39 -0.07562235938105033 1101 40-41 0.021416467295061636 1101 42-43 0.0801981299020369 1101 44-45 0.12325530254231154 1101 46-47 0.08424053451472702 1101 48-49 0.1815613788284196 1101 50-51 0.3015723581957843 1101 52-53 0.21479390656384822 1101 54-55 0.0090930244093812 1101 56-57 0.06077912862427581 1101 58-59 0.10339145733605903 1101 60-61 0.16025258965759548 1101 62-63 -0.07204176203324408 1101 64-65 -0.04261524124348881 1101 66-67 0.08077862099697342 1101 68-69 0.13738253310398107 1101 70-71 -0.06497647340319901 1101 72-73 -0.16076003751167178 1101 74-75 0.01703734547018243 1101 76-77 -0.16328492160220165 1101 78-79 -0.24467611249137633 1101 80-81 -0.30412826247605906 1101 82-83 -0.19189034442126385 1101 84-85 -0.11493694729839277 1101 86-87 -0.13738789821477226 1101 88-89 -0.1678270039081724 1101 90-91 -0.1401895729183238 1101 92-93 0.058742326682899915 1101 94-95 -0.3057458572668459 1101 96-97 -0.4042344931618267 1101 98-99 -0.5664279700990811 1101 100-101 -0.4934789970328204 1102 1 0.32969401698441914 1102 2 0.34650860017320895 1102 3 0.33559298622110134 1102 4 0.15067255741833918 1102 5 0.25863693742337546 1102 6 0.2755345649732135 1102 7 0.3968856409905328 1102 8 0.36205980542278837 1102 9 0.39131668926876273 1102 10-11 0.38062082625614835 1102 12-13 0.43346419059700736 1102 14-15 0.3836504128604119 1102 16-17 0.43206290825430926 1102 18-19 0.45893103167998106 1102 20-21 0.4182492535558211 1102 22-23 0.4816155612592823 1102 24-25 0.5100676803001818 1102 26-27 0.4951432553785793 1102 28-29 0.5287738043045564 1102 30-31 0.43596868823734525 1102 32-33 0.33780998030451315 1102 34-35 0.4231679953372094 1102 36-37 0.5081163509335411 1102 38-39 0.6468020799061307 1102 40-41 0.6488868519241038 1102 42-43 0.21791978823748082 1102 44-45 0.5089643063253106 1102 46-47 0.7282184464926829 1102 48-49 0.8597168180090691 1102 50-51 0.7931948272330729 1102 52-53 0.7447941572317731 1102 54-55 0.837483673660202 1102 56-57 0.753985610061271 1102 58-59 0.5986512224184395 1102 60-61 0.7833832858250886 1102 62-63 0.7043363371100995 1102 64-65 0.6005631444968529 1102 66-67 0.6673154556041894 1102 68-69 0.908961908147063 1102 70-71 0.636447738193958 1102 72-73 0.4497604953247816 1102 74-75 0.5598717939276341 1102 76-77 0.6304185089972485 1102 78-79 0.49910001180649033 1102 80-81 0.4540873787847204 1102 82-83 0.33625339692286005 1102 84-85 0.2761964059491149 1102 86-87 0.24260117684147176 1102 88-89 0.17428353646920414 1102 90-91 0.1310592337979024 1102 92-93 0.22234858168320315 1102 94-95 0.25593255249583535 1102 96-97 0.23285840646029143 1102 98-99 0.21204581734705386 1102 100-101 0.2089666863610553 1103 1 0.1373420978563189 1103 2 0.1643256906802364 1103 3 0.16365806282827577 1103 4 0.15682956263079006 1103 5 0.06565004751657 1103 6 0.176767650846962 1103 7 0.12470536404639887 1103 8 0.04170493783999518 1103 9 0.17283093135795724 1103 10-11 0.01964994224624661 1103 12-13 0.25099117422255546 1103 14-15 0.2564166951749556 1103 16-17 0.1822740386707693 1103 18-19 0.298857320312635 1103 20-21 0.1934906582838778 1103 22-23 0.09429654899161477 1103 24-25 0.22537226620025308 1103 26-27 0.23010718945955233 1103 28-29 0.21101673591093828 1103 30-31 0.22779146504985448 1103 32-33 0.36491838866548676 1103 34-35 0.3966766564228763 1103 36-37 0.24387215570371978 1103 38-39 0.17345242477002643 1103 40-41 0.2695360384665122 1103 42-43 0.09821831927522595 1103 44-45 0.17071337177046786 1103 46-47 0.01887026558393501 1103 48-49 0.06186761040627431 1103 50-51 -0.1962370687285997 1103 52-53 -0.06831014435302052 1103 54-55 0.09761951318002104 1103 56-57 0.21171529994701643 1103 58-59 0.14750132508784475 1103 60-61 0.22571519758204772 1103 62-63 0.36197967939410347 1103 64-65 0.41819911564410717 1103 66-67 0.2494162295735478 1103 68-69 0.23380948356958697 1103 70-71 0.23337081511703417 1103 72-73 0.2509409302218515 1103 74-75 0.17319301753633454 1103 76-77 0.1831010762588683 1103 78-79 0.22666332460594418 1103 80-81 0.18162857817396727 1103 82-83 0.3223314256702956 1103 84-85 0.05249451577905617 1103 86-87 0.14242690210867082 1103 88-89 0.23521861781496511 1103 90-91 0.4079096523731671 1103 92-93 0.2241918923766164 1103 94-95 0.2131257289292563 1103 96-97 0.44046384004108674 1103 98-99 0.33532334064511105 1103 100-101 0.5799633693495245 1104 1 0.1333470606354723 1104 2 0.24236539291348436 1104 3 0.2383571943419227 1104 4 0.17895425245708907 1104 5 0.1921854073180569 1104 6 0.12698684025969698 1104 7 0.12393820110181508 1104 8 0.2237252025215497 1104 9 0.28201724235878345 1104 10-11 0.34731536821811915 1104 12-13 0.40785790292396484 1104 14-15 0.47694502437263964 1104 16-17 0.3667457094730935 1104 18-19 0.4430100291629344 1104 20-21 0.41420354083143707 1104 22-23 0.40505440672527726 1104 24-25 0.3145994167378774 1104 26-27 0.37177333917006194 1104 28-29 0.485289378590835 1104 30-31 0.46065591914415194 1104 32-33 0.45852829354555524 1104 34-35 0.40459642482651503 1104 36-37 0.4531913244382153 1104 38-39 0.3244367093026952 1104 40-41 0.2420251823871027 1104 42-43 0.2594915823025232 1104 44-45 0.33073456697312054 1104 46-47 0.2372299636815356 1104 48-49 0.15835230850387205 1104 50-51 0.07794303879828846 1104 52-53 0.20247511205723612 1104 54-55 0.42103607645545793 1104 56-57 0.2375098615681921 1104 58-59 0.3479650347652665 1104 60-61 0.2908682166060359 1104 62-63 0.22060716078367903 1104 64-65 0.26337694856388083 1104 66-67 0.13643701121126384 1104 68-69 0.07486407414030793 1104 70-71 0.020016286581054032 1104 72-73 0.031128068352536786 1104 74-75 0.12870997369844872 1104 76-77 0.10630465359551167 1104 78-79 0.10064853965970855 1104 80-81 0.16310758975378903 1104 82-83 0.23964997405738941 1104 84-85 0.3236075223960917 1104 86-87 0.4134091188166984 1104 88-89 0.25483865503580816 1104 90-91 0.19277782855182934 1104 92-93 0.2358063464709126 1104 94-95 0.4991182847605202 1104 96-97 0.21519243805597554 1104 98-99 0.3562197426302234 1104 100-101 0.37680114850585156 1105 1 0.21199829251251145 1105 2 0.17562930198384663 1105 3 0.2365802157504291 1105 4 0.09068957649080289 1105 5 0.21754039940692138 1105 6 0.07388938796869837 1105 7 0.052146651487689155 1105 8 0.17722507336012683 1105 9 0.06540042392744994 1105 10-11 0.15723213982844442 1105 12-13 0.18944039267177004 1105 14-15 0.1881330268912862 1105 16-17 0.2582854346821648 1105 18-19 0.17184723330255025 1105 20-21 0.19303327782649404 1105 22-23 -0.006273251578186034 1105 24-25 0.04365054447852401 1105 26-27 -0.006612607260244374 1105 28-29 0.07549198038618243 1105 30-31 0.2299428472012366 1105 32-33 0.19993956368666943 1105 34-35 0.1191375788837945 1105 36-37 0.23388986572143722 1105 38-39 0.25501244633004916 1105 40-41 0.26833021726069006 1105 42-43 0.2091877502446593 1105 44-45 0.4880768891339855 1105 46-47 0.5306589773726458 1105 48-49 0.5047874533261094 1105 50-51 0.529528697037172 1105 52-53 0.5281601721172962 1105 54-55 0.6736309091914165 1105 56-57 0.6770698853016057 1105 58-59 0.3891629867495041 1105 60-61 0.5044446963115448 1105 62-63 0.4516401090545301 1105 64-65 0.35319257063756737 1105 66-67 0.23909359925091422 1105 68-69 0.240453050213155 1105 70-71 0.08890019064640597 1105 72-73 0.10318702246794231 1105 74-75 0.1526694170127314 1105 76-77 0.32024591340370634 1105 78-79 0.34630296424558793 1105 80-81 0.40535538190077247 1105 82-83 0.28034944368831205 1105 84-85 0.29798462126915837 1105 86-87 0.03464527432704401 1105 88-89 -0.019117256520907233 1105 90-91 0.08374086820438364 1105 92-93 0.19825518643991202 1105 94-95 0.1038641596676868 1105 96-97 0.3868948864721311 1105 98-99 0.3230464483682205 1105 100-101 0.4947966641828181 1106 1 0.25096311098952384 1106 2 0.3103669664776092 1106 3 0.24050609284703484 1106 4 0.34106971272459674 1106 5 0.19105342462726327 1106 6 0.27242743821406634 1106 7 0.2396522058225088 1106 8 0.25313708293067094 1106 9 0.27111736037609546 1106 10-11 0.2627894044100998 1106 12-13 0.36666346940704386 1106 14-15 0.4541903036802708 1106 16-17 0.4596223813849676 1106 18-19 0.5695014729080157 1106 20-21 0.4869115463389093 1106 22-23 0.48948103929804887 1106 24-25 0.4656974694522802 1106 26-27 0.4981497160386681 1106 28-29 0.33953455955071377 1106 30-31 0.35878583653202867 1106 32-33 0.3977764249381579 1106 34-35 0.417527188005117 1106 36-37 0.4329528311258599 1106 38-39 0.666985883181539 1106 40-41 0.593063242969329 1106 42-43 0.5489875500444583 1106 44-45 0.621132383652899 1106 46-47 0.5010591336264625 1106 48-49 0.314300380887822 1106 50-51 0.2600230938730306 1106 52-53 0.13883181937431033 1106 54-55 0.11431744939014976 1106 56-57 0.14970154134789482 1106 58-59 0.14067205679516093 1106 60-61 0.2477289561811702 1106 62-63 0.2300096981558326 1106 64-65 0.1468545252751312 1106 66-67 0.2911798318249552 1106 68-69 0.2758357499861006 1106 70-71 0.23205749429151723 1106 72-73 0.12312354435568551 1106 74-75 0.09684530021300475 1106 76-77 -0.06895645970110209 1106 78-79 0.10454950346773373 1106 80-81 0.25114702720461324 1106 82-83 0.15062410859712472 1106 84-85 0.21612178282095584 1106 86-87 0.16341501968215866 1106 88-89 0.2601341900476086 1106 90-91 0.36704661297354235 1106 92-93 0.49811309312708474 1106 94-95 0.4344015263026151 1106 96-97 0.7374244404163193 1106 98-99 0.5442163988064621 1106 100-101 0.6092691854846102 1107 1 0.04806711996064905 1107 2 0.11376571425818582 1107 3 0.09176298472630151 1107 4 0.10107064721670156 1107 5 0.1213234928451854 1107 6 0.056337353175280214 1107 7 -0.023903536631465272 1107 8 0.08981870147099613 1107 9 0.10429276454392777 1107 10-11 0.15239972120292578 1107 12-13 0.13482111822491305 1107 14-15 0.15788294922742097 1107 16-17 0.12594188423517494 1107 18-19 0.16675593510780118 1107 20-21 0.09040047260749162 1107 22-23 0.18561691444990913 1107 24-25 0.09678989072132538 1107 26-27 -0.0323581804196067 1107 28-29 0.016398404051230386 1107 30-31 0.02064734480229191 1107 32-33 0.09417863033952756 1107 34-35 0.19803975692390452 1107 36-37 0.20910646162768387 1107 38-39 0.19745920343198264 1107 40-41 0.0083762978240145 1107 42-43 -0.012564174093476765 1107 44-45 -0.1597390302336592 1107 46-47 0.04573439762047826 1107 48-49 0.23116469263438688 1107 50-51 0.33943342935569376 1107 52-53 0.331605859183675 1107 54-55 0.38707279935744765 1107 56-57 0.3268317160979137 1107 58-59 0.4070990262372689 1107 60-61 0.36063709560739454 1107 62-63 0.1886831866148455 1107 64-65 0.03893003223709712 1107 66-67 0.026872757719729634 1107 68-69 0.13295330909272707 1107 70-71 0.15608375351963133 1107 72-73 0.1461826042566301 1107 74-75 0.2798655786916555 1107 76-77 0.39295224042038157 1107 78-79 0.3987131330350664 1107 80-81 0.23204413962401205 1107 82-83 0.41223564022967807 1107 84-85 0.5203017660000988 1107 86-87 0.6780472970394129 1107 88-89 0.6219073888140798 1107 90-91 0.6128993370180247 1107 92-93 0.5578906840064413 1107 94-95 0.4043370463474716 1107 96-97 0.5255507837487166 1107 98-99 0.5091636000026121 1107 100-101 0.6323609526091793 1108 1 0.13261098143011907 1108 2 -0.02606085356789123 1108 3 0.1403666501286409 1108 4 0.07351191738736418 1108 5 -0.0309628681535159 1108 6 0.08411224874321022 1108 7 0.007073494077516784 1108 8 0.12639490517790364 1108 9 0.18214605621972169 1108 10-11 0.09649637899238428 1108 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-1.437223665650798 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 3135.0 3 580.0 4 530.0 5 559.0 6 644.0 7 793.0 8 933.0 9 1142.0 10 1509.0 11 2237.0 12 3624.0 13 6156.0 14 5650.0 15 6503.0 16 9228.0 17 14020.0 18 11494.0 19 6356.0 20 5899.0 21 5916.0 22 6302.0 23 6884.0 24 7796.0 25 9111.0 26 10415.0 27 12355.0 28 14601.0 29 17724.0 30 21762.0 31 27487.0 32 35058.0 33 46301.0 34 66652.0 35 110537.0 36 141369.0 37 207308.0 38 187925.0 39 29671.0 40 6.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.97031037712964 20.21626970239425 15.637749207301932 19.175670713174178 2 14.735212744381473 22.902499971299886 39.83939415971805 22.52289312460059 3 19.50180591766929 28.04879565622982 32.505752625158465 19.943645800942424 4 11.65528531829981 18.37052005920789 39.76209680891677 30.21209781357552 5 11.515127156921286 39.48266424754643 34.83781942339198 14.164389172140309 6 26.80843128066333 38.457580597343224 18.461406042290292 16.272582079703145 7 23.951300921476083 37.01235170069003 22.27839384853745 16.757953529296437 8 24.15813403672936 33.17291415214701 21.35035204869194 21.31859976243169 9 25.517092308604596 18.482762314477057 23.120381652667763 32.879763724250594 10-11 23.32369434969378 27.320660628234744 27.801494002613858 21.554151019457613 12-13 26.71022700374283 26.38290282273471 27.963431505083925 18.943438668438535 14-15 23.194489805384432 26.895193355556074 29.626477166059935 20.28383967299956 16-17 18.77954949203685 30.354318942040315 30.16060195247215 20.705529613450683 18-19 18.46822418107109 29.26566122627 33.52352722876447 18.742587363894437 20-21 20.39080990783796 27.33866382016948 32.56360261713226 19.706923654860304 22-23 19.60978269708174 27.333626705825516 32.85818150509655 20.19840909199619 24-25 20.310005802503447 27.506545156918733 33.04810337544971 19.135345665128103 26-27 20.05408102203852 26.71021784998998 34.19943010676051 19.036271021210993 28-29 18.791216938556357 28.01089631519912 34.144545982880636 19.05334076336389 30-31 19.23922037546335 28.123687671888074 33.29322013631472 19.343871816333852 32-33 19.124053589627948 28.473543074435405 32.89636606156026 19.50603727437639 34-35 19.96441902139365 27.475974929360586 32.88062107072762 19.67898497851814 36-37 20.096817070383256 27.27531159903269 33.558048945394056 19.069822385189994 38-39 19.983245560553616 27.624086066931348 33.54814861043768 18.844519762077358 40-41 19.09063506173598 28.19359620003571 33.73289084894412 18.982877889284186 42-43 18.72809384524676 28.874832672987804 32.256230710580446 20.140842771184992 44-45 19.259433543247727 28.494690518319693 31.92080073177934 20.32507520665324 46-47 19.959033965989356 28.67142235011171 31.324995762713897 20.04454792118504 48-49 20.003323186090256 28.56653023354987 31.362520703881547 20.067625876478328 50-51 19.5154851607246 29.145995534987534 32.00191316515984 19.336606139128023 52-53 19.444293712492755 29.742017862776727 31.10388771584749 19.709800708883034 54-55 18.721286002934782 28.871683965489424 31.643977167090494 20.7630528644853 56-57 19.881116746005382 29.549094309695583 30.278744841703425 20.291044102595617 58-59 20.103235542440185 28.854641303528716 30.82829867269274 20.213824481338357 60-61 19.634667923112428 28.876021621870603 31.651109833767237 19.838200621249737 62-63 20.004273145202912 28.809929060988342 31.36315732616341 19.82264046764533 64-65 19.58131656883588 29.36498751162756 31.487362516249775 19.566333403286787 66-67 19.307565514578325 28.974195680197028 30.872586368203358 20.845652437021283 68-69 18.83781810496383 29.426282266819094 31.307148415359343 20.428751212857733 70-71 19.84318427001048 29.416446438743975 30.159121278580397 20.581248012665146 72-73 19.492054130560177 28.706035249124582 30.959137929958008 20.84277269035723 74-75 19.18484749810051 29.969867511183217 30.380983393031585 20.46430159768469 76-77 19.713545442934286 28.674649506346288 30.591645003394742 21.020160047324683 78-79 19.284034065269363 28.75217228500379 31.288873100150738 20.674920549576104 80-81 19.743812310182886 29.496007059300233 30.3238329158117 20.43634771470518 82-83 19.37612892260092 29.12814765947517 30.66175390279526 20.833969515128643 84-85 19.278691169310815 28.80880428133676 30.35904113574884 21.553463413603595 86-87 19.520751018981457 29.18380173590555 30.44499084609191 20.850456399021088 88-89 19.956265974707442 28.378619113036706 30.150019999145293 21.515094913110556 90-91 19.45316210592235 28.14048318709273 30.828521744475694 21.577832962509227 92-93 19.84295419016951 29.132093776039227 30.41708389464003 20.607868139151233 94-95 19.45492287904156 28.763471072316086 30.32769563133329 21.45391041730906 96-97 18.99384166011516 28.435028625738816 30.391404930417913 22.179724783728112 98-99 19.55674471613594 28.63365161149029 29.673062487122632 22.136541185251144 100-101 19.87248711114355 27.686612923284354 30.63238345290268 21.80851651266942 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 13631.0 1 8101.5 2 2669.0 3 2614.0 4 2113.0 5 2145.5 6 3692.0 7 6866.5 8 10009.0 9 10340.0 10 7404.5 11 4848.0 12 4330.0 13 4202.0 14 4152.0 15 4103.5 16 4012.0 17 3932.0 18 3873.5 19 3872.5 20 4062.5 21 4419.5 22 4851.0 23 5319.5 24 5742.5 25 6657.0 26 8015.0 27 9440.0 28 10574.5 29 11604.5 30 13228.5 31 15266.5 32 17890.0 33 20731.0 34 23322.0 35 25190.0 36 27624.0 37 30421.5 38 30431.5 39 28779.0 40 28077.0 41 28039.0 42 27322.5 43 28179.5 44 30258.5 45 30218.0 46 30803.5 47 38654.0 48 66086.5 49 66373.0 50 39955.5 51 30740.5 52 25513.0 53 23052.0 54 21567.0 55 19453.5 56 17545.0 57 16155.0 58 14814.5 59 13081.5 60 11233.5 61 9233.5 62 7362.0 63 5522.5 64 3829.5 65 2784.5 66 1973.0 67 1437.5 68 1028.5 69 668.0 70 435.5 71 293.5 72 238.5 73 170.5 74 99.0 75 83.0 76 64.0 77 37.0 78 26.5 79 23.5 80 13.5 81 10.5 82 11.0 83 8.5 84 5.0 85 4.5 86 4.0 87 4.0 88 2.5 89 1.0 90 2.0 91 1.5 92 0.5 93 1.0 94 1.5 95 1.5 96 1.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.3073108437235942 2 0.08411618739557165 3 0.09529981685611925 4 0.09950562622589784 5 0.1112627751459607 6 0.05180792450954528 7 0.05419758892419219 8 0.05524904126663684 9 0.058690158023728414 10-11 0.056539460050546185 12-13 0.05472331509541452 14-15 0.05223806410418172 16-17 0.054914488248586274 18-19 0.06308714054667874 20-21 0.06466431906034573 22-23 0.0819176961340965 24-25 0.08923006924291609 26-27 0.08492867329655161 28-29 0.08263459545849057 30-31 0.07618250153894388 32-33 0.06772308951109378 34-35 0.3057814584982202 36-37 0.47124182256837305 38-39 0.4451466871604287 40-41 0.4280366899515567 42-43 0.4586243944590373 44-45 0.4659367675678569 46-47 0.46087067900880546 48-49 0.47817184937084917 50-51 0.47463514603717166 52-53 0.4737748668478988 54-55 0.4653154548200487 56-57 0.4440952348179841 58-59 0.42789331008667786 60-61 0.38989764589379183 62-63 0.45733397567512796 64-65 0.47826743594743504 66-67 0.4880650600474874 68-69 0.5010648344631666 70-71 0.49752813112948924 72-73 0.48715698756992154 74-75 0.4877783003177298 76-77 0.46407282932443233 78-79 0.4437128885116405 80-81 0.4501171891428943 82-83 0.45910232734196665 84-85 0.4602493662609972 86-87 0.4601059863961184 88-89 0.46622352729761457 90-91 0.48008358090256664 92-93 0.4850540828850323 94-95 0.49179293653433664 96-97 0.48400263054258763 98-99 0.487634920452851 100-101 0.46507648837858406 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1046172.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 40.05795052201414 #Duplication Level Percentage of deduplicated Percentage of total 1 71.5722707727367 28.670384813624857 2 14.160611733352152 11.344901683521465 3 5.3801135854409186 6.465489714252253 4 2.7348037399373553 4.382025316073192 5 1.4945050451569877 2.9933404576899556 6 0.9320216526938648 2.240092634943401 7 0.6611933711180515 1.854023594201145 8 0.46591892344548835 1.4931005746119572 9 0.3564456428513609 1.2850633732614603 >10 1.9866481275566232 14.746529489652202 >50 0.1585348363284176 4.37776206249686 >100 0.08332380966858016 5.80037485425961 >500 0.0035812525562000943 0.9751293826805386 >1k 0.007878755623640207 6.0551556105975815 >5k 0.0016712511928933772 4.846668470586667 >10k+ 4.775003408266792E-4 2.469957967546841 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 14663 1.4015859724787127 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 11163 1.0670329544281436 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 8813 0.842404499451333 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 8202 0.7840011011573623 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7992 0.7639279200743281 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7824 0.7478693752079009 No Hit GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 6746 0.6448270456483255 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 5552 0.5306966732047885 No Hit GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA 5548 0.530314326898445 No Hit GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT 4688 0.4481098710345909 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 4062 0.3882726740918319 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 2900 0.277201072099043 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2745 0.26238515272823204 No Hit GCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCT 2587 0.24728247362766353 No Hit GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT 2404 0.22979013011244806 No Hit CTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCA 2360 0.2255843207426695 No Hit CTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTG 2357 0.22529756101291182 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2279 0.21784180803921344 No Hit ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC 2252 0.21526097047139475 No Hit TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT 2079 0.19872449272203807 No Hit TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC 2071 0.19795980010935108 No Hit GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA 2049 0.19585689542446177 No Hit ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC 1958 0.18715851695514696 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1954 0.18677617064880345 No Hit TGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCAC 1869 0.1786513116390039 No Hit GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG 1851 0.17693075326045812 No Hit GTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTG 1818 0.1737763962331242 No Hit GGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGT 1558 0.1489238863207962 No Hit TATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGG 1524 0.14567394271687636 No Hit GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1454 0.138982882355865 No Hit GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA 1405 0.13429914010315702 No Hit CCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCAC 1403 0.13410796694998528 No Hit GATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATA 1361 0.13009333073337845 No Hit ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1339 0.12799042604848915 No Hit ACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCA 1221 0.11671121001135569 No Hit TGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGAT 1219 0.11652003685818392 No Hit GTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTG 1196 0.11432154559670876 No Hit ATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTG 1192 0.11393919929036525 No Hit AAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGC 1077 0.10294674298298942 No Hit CAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAG 1064 0.10170411748737301 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 1.9117315317175378E-4 0.0 10-11 0.0 0.0 0.0 2.8675972975763066E-4 0.0 12-13 0.0 0.0 0.0 3.8234630634350757E-4 0.0 14-15 0.0 0.0 0.0 4.3013959463644605E-4 0.0 16-17 9.558657658587689E-5 0.0 0.0 5.735194595152613E-4 0.0 18-19 1.4337986487881533E-4 0.0 0.0 6.213127478081999E-4 0.0 20-21 2.3896644146469224E-4 0.0 0.0 9.080724775658304E-4 0.0 22-23 2.8675972975763066E-4 0.0 0.0 0.001386005360495215 0.0 24-25 2.8675972975763066E-4 0.0 0.0 0.002246284549768107 0.0 26-27 2.8675972975763066E-4 0.0 0.0 0.003823463063435076 0.0 28-29 2.8675972975763066E-4 0.0 0.0 0.011422595902012288 0.0 30-31 2.8675972975763066E-4 0.0 0.0 0.02714658775038904 0.0 32-33 2.8675972975763066E-4 0.0 0.0 0.056874013068596754 0.0 34-35 2.8675972975763066E-4 0.0 0.0 0.1011783913161507 0.0 36-37 2.8675972975763066E-4 0.0 0.0 0.16479125803405176 0.0 38-39 2.8675972975763066E-4 0.0 0.0 0.25469043331306895 0.0 40-41 2.8675972975763066E-4 0.0 0.0 0.354817372286775 0.0 42-43 2.8675972975763066E-4 0.0 0.0 0.45766852869317853 0.0 44-45 2.8675972975763066E-4 0.0 0.0 0.5842251560928795 0.0 46-47 2.8675972975763066E-4 0.0 0.0 0.7328622826839182 0.0 48-49 2.8675972975763066E-4 0.0 0.0 0.8956940158979594 0.0 50-51 2.8675972975763066E-4 0.0 0.0 1.0648822564549616 0.0 52-53 2.8675972975763066E-4 0.0 0.0 1.2208795494431126 0.0 54-55 2.8675972975763066E-4 0.0 0.0 1.3964720906313683 0.0 56-57 2.8675972975763066E-4 0.0 0.0 1.6154609375896123 0.0 58-59 2.8675972975763066E-4 0.0 0.0 1.861787545451417 0.0 60-61 2.8675972975763066E-4 0.0 0.0 2.1618338093544844 0.0 62-63 2.8675972975763066E-4 0.0 0.0 2.453755214247753 0.0 64-65 2.8675972975763066E-4 0.0 0.0 2.73688265409512 0.0 66-67 2.8675972975763066E-4 0.0 0.0 3.062163774216859 0.0 68-69 2.8675972975763066E-4 0.0 0.0 3.437532260469598 0.0 70-71 2.8675972975763066E-4 0.0 0.0 3.8725467705119234 0.0 72-73 3.8234630634350757E-4 0.0 0.0 4.319652982492363 0.0 74-75 3.8234630634350757E-4 0.0 0.0 4.7920896372680595 0.0 76-77 3.8234630634350757E-4 0.0 0.0 5.261419728304714 0.0 78-79 4.779328829293845E-4 0.0 0.0 5.822656312728691 0.0 80-81 4.779328829293845E-4 0.0 0.0 6.429726660625596 0.0 82-83 4.779328829293845E-4 0.0 0.0 7.03015374144978 0.0 84-85 4.779328829293845E-4 0.0 0.0 7.707766982867062 0.0 86-87 4.779328829293845E-4 0.0 0.0 8.413482677800591 0.0 88-89 4.779328829293845E-4 0.0 0.0 9.124216668004879 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CCGAACA 70 2.0865872E-4 33.83554 4 CTTTGCG 545 0.0 29.551777 2 TTTGCGT 580 0.0 26.951761 3 GAGTACT 8125 0.0 24.972525 12-13 TTGCGTT 635 0.0 24.617353 4 CTACGCA 175 1.5258411E-7 24.361591 3 GTACACG 165 2.442015E-6 23.016714 1 GTACTTT 8860 0.0 22.982141 14-15 TAATACC 395 0.0 22.785454 4 AGTACTT 8295 0.0 22.519855 12-13 CTCTACG 190 3.3050856E-7 22.486723 1 GCTTTGC 720 0.0 22.41732 1 GTATAGA 170 3.1646905E-6 22.339752 1 CATATAG 170 3.224457E-6 22.291653 3 GTACATG 7135 0.0 22.155806 1 ACGCATT 195 4.3337423E-7 21.862967 5 TACGCAT 195 4.3337423E-7 21.862967 4 TACATGG 7290 0.0 21.313143 2 ACTTTTT 9845 0.0 20.902206 16-17 CATGGGT 545 0.0 20.860077 4 >>END_MODULE