##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938879_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1046172 Sequences flagged as poor quality 0 Sequence length 101 %GC 40 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.948321117368845 31.0 31.0 34.0 30.0 34.0 2 32.18880260607242 33.0 31.0 34.0 30.0 34.0 3 32.233652783672284 34.0 31.0 34.0 30.0 34.0 4 35.86296421620919 37.0 35.0 37.0 35.0 37.0 5 35.80003957284271 37.0 35.0 37.0 35.0 37.0 6 35.87246838951912 37.0 35.0 37.0 35.0 37.0 7 35.805628519975684 37.0 35.0 37.0 33.0 37.0 8 35.79256279082216 37.0 35.0 37.0 33.0 37.0 9 37.387687684243126 39.0 37.0 39.0 34.0 39.0 10-11 37.292117835308154 39.0 37.0 39.0 34.0 39.0 12-13 37.184733963440046 39.0 37.0 39.0 33.0 39.0 14-15 38.292032763254994 40.0 38.0 41.0 33.0 41.0 16-17 38.403916851148765 40.0 38.0 41.0 33.5 41.0 18-19 38.502436023904295 40.0 38.0 41.0 34.0 41.0 20-21 38.493101516767794 40.0 38.0 41.0 34.0 41.0 22-23 38.393756475990564 40.0 38.0 41.0 34.0 41.0 24-25 38.27445964908256 40.0 37.5 41.0 33.5 41.0 26-27 38.02376186707348 40.0 37.0 41.0 33.0 41.0 28-29 37.8188457538531 40.0 37.0 41.0 33.0 41.0 30-31 37.52677571183324 39.5 36.0 41.0 33.0 41.0 32-33 37.25846036789362 39.0 35.0 41.0 32.0 41.0 34-35 37.06428436241842 39.0 35.0 41.0 31.0 41.0 36-37 36.86247051154112 39.0 35.0 41.0 31.0 41.0 38-39 36.63583282672448 39.0 35.0 41.0 31.0 41.0 40-41 36.41640714911124 39.0 35.0 41.0 30.0 41.0 42-43 36.1599603124534 39.0 35.0 40.5 30.0 41.0 44-45 35.78359199060958 38.5 35.0 40.0 29.5 41.0 46-47 35.26242195356022 38.0 35.0 40.0 26.5 41.0 48-49 34.748173818454326 38.0 34.0 40.0 24.0 41.0 50-51 34.42109710449142 38.0 34.0 40.0 23.0 41.0 52-53 34.126614457278535 38.0 33.5 40.0 23.0 41.0 54-55 33.82120148503306 38.0 33.0 40.0 22.0 41.0 56-57 33.69266334790073 37.5 33.0 40.0 22.0 41.0 58-59 33.41096683910485 37.0 33.0 40.0 20.5 41.0 60-61 32.982679712322636 36.5 32.5 39.5 19.5 41.0 62-63 32.519508264415414 36.0 32.0 39.0 17.0 40.0 64-65 32.043842695082645 35.5 31.0 39.0 13.0 40.0 66-67 31.53940938966059 35.0 31.0 38.0 8.0 40.0 68-69 31.017581239031443 35.0 30.0 38.0 7.5 40.0 70-71 30.414801294624596 34.5 29.5 37.0 3.5 39.0 72-73 29.843033459125266 34.0 29.0 36.5 2.0 39.0 74-75 29.28549894281246 34.0 28.5 36.0 2.0 38.5 76-77 27.44434758338017 31.5 26.0 34.0 2.0 36.0 78-79 28.40497547248445 33.0 27.5 35.0 2.0 37.0 80-81 28.375015293852254 34.0 28.0 35.0 2.0 37.0 82-83 27.909130620968636 33.0 26.5 35.0 2.0 36.0 84-85 27.393301483886013 33.0 26.0 35.0 2.0 36.0 86-87 26.631199267424478 32.5 25.0 35.0 2.0 35.0 88-89 25.807500583078117 32.0 23.0 34.0 2.0 35.0 90-91 25.08870051960863 31.0 19.5 34.0 2.0 35.0 92-93 24.586981395028733 31.0 18.0 34.0 2.0 35.0 94-95 23.893940958083377 31.0 8.5 34.0 2.0 35.0 96-97 22.825404426805534 30.5 2.0 34.0 2.0 35.0 98-99 21.39827533139866 30.0 2.0 34.0 2.0 35.0 100-101 16.03485421135339 13.5 2.0 29.0 2.0 32.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.09228291285515766 1101 2 0.14783843561912136 1101 3 0.022614578427770482 1101 4 0.04522251469836647 1101 5 0.04686717985774891 1101 6 0.11721791030426232 1101 7 0.07371842230678283 1101 8 0.04093226403347927 1101 9 0.1188074591979813 1101 10-11 0.19835523316319836 1101 12-13 0.12414899821346381 1101 14-15 0.16966095265928516 1101 16-17 0.17367713119142536 1101 18-19 -0.027981792367455682 1101 20-21 0.05996504019047677 1101 22-23 0.28066119133167433 1101 24-25 0.18392114140473126 1101 26-27 0.1765115008204461 1101 28-29 0.1872887767423066 1101 30-31 0.17447400776526223 1101 32-33 0.2955209771287528 1101 34-35 0.33012150510791827 1101 36-37 0.45432470417581783 1101 38-39 0.419954967993597 1101 40-41 0.49580237574092223 1101 42-43 0.36041917961381387 1101 44-45 0.4100385905397772 1101 46-47 0.5106989484141593 1101 48-49 0.49801730146628387 1101 50-51 0.522901431865499 1101 52-53 0.5099380927692465 1101 54-55 0.6035732845826374 1101 56-57 0.26067317813233615 1101 58-59 0.17146994977067465 1101 60-61 0.12058936100446971 1101 62-63 0.26077388591166795 1101 64-65 0.12659680062705547 1101 66-67 0.39952268204703856 1101 68-69 0.36285391215919205 1101 70-71 0.4227747766988088 1101 72-73 0.30047058490059086 1101 74-75 0.406897457866247 1101 76-77 0.36825558302023964 1101 78-79 0.35825487127630495 1101 80-81 0.20144988273653297 1101 82-83 0.31914468599516255 1101 84-85 0.14839773871317874 1101 86-87 0.38778932451549153 1101 88-89 0.4061577089649333 1101 90-91 0.13562764444268183 1101 92-93 -0.026044815320954484 1101 94-95 -0.3979882931794272 1101 96-97 0.028900246225806114 1101 98-99 0.5211605074941765 1101 100-101 0.9125829287389386 1102 1 0.11133680854805661 1102 2 0.1603498984544629 1102 3 0.20791353126251266 1102 4 0.010599007478091949 1102 5 0.14730668428725835 1102 6 0.12353787537786332 1102 7 0.21970846661233878 1102 8 0.2172155271449796 1102 9 0.11659322585538234 1102 10-11 0.2625847112934636 1102 12-13 0.2874763851465687 1102 14-15 0.4171412177823939 1102 16-17 0.32822539625604463 1102 18-19 0.33156322445862685 1102 20-21 0.20812283982743196 1102 22-23 0.2117134117272741 1102 24-25 0.35075422372198517 1102 26-27 0.23625767865680558 1102 28-29 0.2113868422125904 1102 30-31 0.300615852388745 1102 32-33 0.19798765396139828 1102 34-35 0.22084073405338245 1102 36-37 0.15636931888877825 1102 38-39 0.2890164881692314 1102 40-41 0.43279803709690867 1102 42-43 0.2821204419357173 1102 44-45 0.14134137441597971 1102 46-47 0.09591298748910759 1102 48-49 0.32776416863944036 1102 50-51 0.2591632628693574 1102 52-53 0.4768776045099088 1102 54-55 0.27295900194202716 1102 56-57 0.3963753929923399 1102 58-59 0.3457087026141821 1102 60-61 0.5269079682934787 1102 62-63 0.5523252279250741 1102 64-65 0.6594656323683026 1102 66-67 0.5010196917875724 1102 68-69 0.5589770193552397 1102 70-71 0.6673274493411192 1102 72-73 0.8967500462152209 1102 74-75 0.7238727942312693 1102 76-77 0.41626319079178487 1102 78-79 0.5751546939287451 1102 80-81 0.8389589955600805 1102 82-83 0.9313368605603074 1102 84-85 1.3189642064469957 1102 86-87 1.4940124691028416 1102 88-89 1.229273365056894 1102 90-91 1.208974646140959 1102 92-93 1.2700650273640335 1102 94-95 1.2364840821707261 1102 96-97 1.5448818931558534 1102 98-99 2.1711857831766714 1102 100-101 1.7116856420103446 1103 1 0.08868951744080178 1103 2 0.0030508935579263152 1103 3 0.11000482805606993 1103 4 0.07738361233251112 1103 5 0.08579876031527078 1103 6 0.07530852772346464 1103 7 0.12836849925594152 1103 8 -0.019740193383633198 1103 9 0.10168457529991315 1103 10-11 4.607708943709099E-4 1103 12-13 0.08541931148785409 1103 14-15 0.11688217497714959 1103 16-17 0.15000078718954768 1103 18-19 0.2968130533429516 1103 20-21 0.14121239913875172 1103 22-23 0.10593286099805965 1103 24-25 0.1967374807113984 1103 26-27 0.18363881907696822 1103 28-29 0.3282314691804231 1103 30-31 0.267909592187614 1103 32-33 0.21181959204384526 1103 34-35 -0.020022741964098145 1103 36-37 0.07988956318931173 1103 38-39 0.1895535281313201 1103 40-41 0.33751977668517696 1103 42-43 0.4421167563673407 1103 44-45 0.2777663730997091 1103 46-47 0.18522747177279797 1103 48-49 0.17840335149627862 1103 50-51 0.3871125073278492 1103 52-53 0.45414238627161296 1103 54-55 0.5615053084760149 1103 56-57 0.8198061306325286 1103 58-59 0.8238538087058984 1103 60-61 0.7719893140524405 1103 62-63 1.0552773681872694 1103 64-65 0.9586895569721037 1103 66-67 0.6547779185026847 1103 68-69 0.43626602056621167 1103 70-71 0.8512710074941232 1103 72-73 0.6856796518594024 1103 74-75 0.6689341326190963 1103 76-77 0.5922527658984045 1103 78-79 1.0233356026846003 1103 80-81 0.8740961513543226 1103 82-83 0.991475332628962 1103 84-85 1.2238454784945922 1103 86-87 1.4922555060111762 1103 88-89 1.4896582436963932 1103 90-91 1.3799034093695148 1103 92-93 1.1635036626507151 1103 94-95 1.535266265080221 1103 96-97 0.8634334647875086 1103 98-99 1.1951772537184517 1103 100-101 1.3498602326490001 1104 1 -0.005054279085250357 1104 2 0.12244915658521904 1104 3 0.08261338885011327 1104 4 0.0066371276344128205 1104 5 0.17607274708119292 1104 6 0.22794810588723635 1104 7 0.21767577799870708 1104 8 0.17429930206715483 1104 9 0.06150570846782699 1104 10-11 0.2058557254022304 1104 12-13 0.19564005176907528 1104 14-15 0.15449383750816992 1104 16-17 0.14340494351709054 1104 18-19 0.11597299957951179 1104 20-21 0.12014074901468064 1104 22-23 0.18454741848358225 1104 24-25 0.20711124001661574 1104 26-27 0.18135749153353942 1104 28-29 0.2079828132664474 1104 30-31 0.14588312403211745 1104 32-33 0.050054703706379655 1104 34-35 0.33862903636509856 1104 36-37 0.3194542861008074 1104 38-39 0.43964658024049896 1104 40-41 0.6352844582401787 1104 42-43 0.5244947547130892 1104 44-45 0.6032957362097164 1104 46-47 0.3342891963385526 1104 48-49 0.2493545508345747 1104 50-51 0.44163225505323567 1104 52-53 0.5615519189432376 1104 54-55 0.607119969352766 1104 56-57 0.3492074953967972 1104 58-59 0.3638000452650445 1104 60-61 0.4882765348961158 1104 62-63 0.46846440292672753 1104 64-65 0.4148888952682199 1104 66-67 0.43595606159614775 1104 68-69 0.5528499742469393 1104 70-71 0.550603100132669 1104 72-73 0.33345829123078374 1104 74-75 0.3214801417175259 1104 76-77 0.4148690810348796 1104 78-79 0.5737181502445701 1104 80-81 0.8784318626859999 1104 82-83 0.9111051920168869 1104 84-85 0.7848576786600177 1104 86-87 0.753755712793641 1104 88-89 0.8792276192133492 1104 90-91 0.9532284714042554 1104 92-93 1.2336215286556786 1104 94-95 1.3580841931199217 1104 96-97 0.9532287501473107 1104 98-99 1.0406921420559208 1104 100-101 1.0952179663131858 1105 1 0.10630405505534313 1105 2 0.023049353556380936 1105 3 0.09027124832248745 1105 4 -0.015552400603503713 1105 5 -0.08518145066494043 1105 6 -0.09927528686034748 1105 7 0.13321026409770553 1105 8 0.056258882615445316 1105 9 0.1332114868268306 1105 10-11 0.07512620555980476 1105 12-13 0.0886363747049117 1105 14-15 0.05146136955634262 1105 16-17 0.048721045909800864 1105 18-19 0.12231855884846254 1105 20-21 0.15612885405520416 1105 22-23 0.26987064493584256 1105 24-25 0.05880416277808109 1105 26-27 -0.07714042170227486 1105 28-29 0.12488812583708153 1105 30-31 0.18278292706094845 1105 32-33 0.19413729436154625 1105 34-35 0.27735001540865767 1105 36-37 0.3063252296249743 1105 38-39 0.187004825582612 1105 40-41 0.22968732885273369 1105 42-43 0.4401886744392627 1105 44-45 0.39595533128866833 1105 46-47 0.3617193482646712 1105 48-49 0.29356004665297775 1105 50-51 0.389055989271327 1105 52-53 0.1879177000469241 1105 54-55 0.30080306777377075 1105 56-57 0.5621654129918099 1105 58-59 0.43608604093813597 1105 60-61 0.4271660292291628 1105 62-63 0.4832145361244429 1105 64-65 0.4873767056592513 1105 66-67 0.378253701978462 1105 68-69 0.18552296982316108 1105 70-71 0.485269621492737 1105 72-73 0.5660785122582617 1105 74-75 0.5645836282685899 1105 76-77 0.19528810893374526 1105 78-79 0.38737656672556753 1105 80-81 0.343932141190308 1105 82-83 0.18396474511837724 1105 84-85 0.3309572056063175 1105 86-87 0.5061986799543483 1105 88-89 0.8297552637934125 1105 90-91 0.9756299050960102 1105 92-93 1.1359976951447486 1105 94-95 1.0205838903978481 1105 96-97 0.9504820518360972 1105 98-99 0.9362429347841363 1105 100-101 0.38986947265824057 1106 1 0.04193529605243285 1106 2 0.14894207554666394 1106 3 0.09619619766207421 1106 4 0.08656435029373455 1106 5 0.1058612994021999 1106 6 0.15260683916812212 1106 7 0.1554354160789586 1106 8 0.15416849954944922 1106 9 0.1279760074450067 1106 10-11 0.28979160266422355 1106 12-13 0.17597590936151875 1106 14-15 0.14457448266946216 1106 16-17 0.1963047271520253 1106 18-19 0.10575614277384915 1106 20-21 0.17873585129634506 1106 22-23 0.1883593775709116 1106 24-25 0.3727224713305404 1106 26-27 0.341162271234559 1106 28-29 0.3197761846275782 1106 30-31 0.24713410750969445 1106 32-33 0.4148589916686163 1106 34-35 0.35065893408342674 1106 36-37 0.2739733655657943 1106 38-39 0.46092313413507213 1106 40-41 0.4452746234644138 1106 42-43 0.39560018350930903 1106 44-45 0.3952272861703747 1106 46-47 0.23168150554390365 1106 48-49 0.3268211125969671 1106 50-51 0.40674978062365597 1106 52-53 0.35443006607148675 1106 54-55 0.34609567745661707 1106 56-57 0.32378361656122934 1106 58-59 0.3576749469172853 1106 60-61 0.25292740035637706 1106 62-63 0.3223693006450574 1106 64-65 0.015475047903933614 1106 66-67 0.3748029341374526 1106 68-69 0.2738019805411973 1106 70-71 0.429857624129518 1106 72-73 0.46116432978310584 1106 74-75 0.5006289418748828 1106 76-77 0.421220870964067 1106 78-79 0.5827602430848522 1106 80-81 0.5971980900660157 1106 82-83 0.43854224945198084 1106 84-85 0.5830825016340526 1106 86-87 0.5321929669242458 1106 88-89 0.77623735564136 1106 90-91 0.826957818375142 1106 92-93 0.653222236759536 1106 94-95 0.957405101853201 1106 96-97 1.1155910632865726 1106 98-99 0.982381756532714 1106 100-101 1.2060578561636959 1107 1 0.11068774909420753 1107 2 0.06393506685589045 1107 3 0.09846047202895392 1107 4 0.05853893210851879 1107 5 0.09949460470076588 1107 6 0.16984728398636406 1107 7 0.14312535159900364 1107 8 0.08481329910089386 1107 9 -0.011295088714227575 1107 10-11 0.17785393328753685 1107 12-13 0.12138895487128565 1107 14-15 0.10422965508325177 1107 16-17 0.148312016190431 1107 18-19 0.12112339817053908 1107 20-21 0.10981098446147541 1107 22-23 0.03353085687191282 1107 24-25 0.08577078457229703 1107 26-27 0.13880828562573555 1107 28-29 -0.009737879478585398 1107 30-31 0.15674395705646305 1107 32-33 0.074452954849626 1107 34-35 0.04082901164628083 1107 36-37 0.06226651573867059 1107 38-39 -0.003461502814751327 1107 40-41 -0.05977023215655208 1107 42-43 -0.11563007534500258 1107 44-45 -0.10625808902820211 1107 46-47 0.1219796085249314 1107 48-49 0.19713603643585742 1107 50-51 0.03984902368505061 1107 52-53 0.03944595243724791 1107 54-55 0.09555040131420611 1107 56-57 0.037800530868310034 1107 58-59 0.22684231445303027 1107 60-61 0.49989824420275397 1107 62-63 0.42060451230751994 1107 64-65 0.2715660674348186 1107 66-67 0.47276200338331975 1107 68-69 0.30895243376986414 1107 70-71 0.32216254890290585 1107 72-73 0.16441823128763744 1107 74-75 0.3095397913282021 1107 76-77 0.1492981534610287 1107 78-79 0.45910778069815805 1107 80-81 0.43245600988659305 1107 82-83 0.40878007769233093 1107 84-85 0.5159439164550967 1107 86-87 0.7881573627750988 1107 88-89 0.6097982378019076 1107 90-91 0.5230436981994586 1107 92-93 0.7549183399274639 1107 94-95 0.888671805899552 1107 96-97 1.4056527397654044 1107 98-99 1.494382971372449 1107 100-101 1.5080609399876401 1108 1 0.08138143936040976 1108 2 0.04457238109746697 1108 3 0.0648817665639001 1108 4 0.03832695518156726 1108 5 0.06775282764546375 1108 6 0.029713280443168344 1108 7 -0.008274249503152475 1108 8 0.1573037589862949 1108 9 0.2105486032812962 1108 10-11 0.10899457744221763 1108 12-13 0.175510611027498 1108 14-15 0.1124180132612338 1108 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-1.3586169990646653 2316 74-75 -1.2450228663673393 2316 76-77 -1.3092921652007767 2316 78-79 -1.3723448872767996 2316 80-81 -1.6373855937018575 2316 82-83 -1.452155816149368 2316 84-85 -1.7729309550522352 2316 86-87 -1.8506497580547254 2316 88-89 -1.9034598689170927 2316 90-91 -1.8213516854095495 2316 92-93 -1.7891663250571206 2316 94-95 -1.5463459494504157 2316 96-97 -1.461112048572133 2316 98-99 -1.882462650532048 2316 100-101 -1.2913853734410132 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 3.0 3 3.0 4 21.0 5 85.0 6 230.0 7 362.0 8 621.0 9 1145.0 10 1839.0 11 2665.0 12 3800.0 13 5036.0 14 6292.0 15 8633.0 16 11184.0 17 16132.0 18 15382.0 19 10014.0 20 8978.0 21 9410.0 22 10174.0 23 11396.0 24 12919.0 25 14899.0 26 17369.0 27 20275.0 28 24028.0 29 28740.0 30 34450.0 31 40588.0 32 49481.0 33 62844.0 34 87625.0 35 120481.0 36 152797.0 37 177492.0 38 76615.0 39 2164.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.304638309344064 21.254368771831867 16.606849389978905 18.834143528845168 2 15.147222445257569 24.146220697934947 39.10427730812906 21.60227954867842 3 19.710621198043913 28.202628248509804 32.91112742455351 19.175623128892763 4 12.045342448469276 19.38964147386854 38.863303548556075 29.70171252910611 5 11.20857755703651 40.13355356480579 34.70136841743041 13.9565004607273 6 26.51772366303055 39.374309386984166 17.741250960645093 16.366715989340186 7 23.72888970456101 37.39021881679112 21.911024191050803 16.96986728759707 8 24.33787178398963 33.99010870105489 20.676905900750544 20.99511361420493 9 25.17129114524189 18.644735282534803 22.617408992020433 33.56656458020287 10-11 23.442368941244844 27.85106081982695 27.39372684415182 21.312843394776383 12-13 26.899114103608202 26.626453393896988 27.73520988900487 18.739222613489943 14-15 23.01460382884519 27.327455351168652 29.412310115813582 20.245630704172576 16-17 18.561958428324946 30.640637770098788 30.14954905868689 20.647854742889372 18-19 18.330785943762432 29.38382804307908 33.54840374738689 18.73698226577159 20-21 20.314871741931537 27.29087568774542 32.94983998807079 19.444412582252248 22-23 19.54621228631621 27.288629403195653 33.16572227129 19.999436039198144 24-25 20.194069611946805 27.69011949289361 33.43277533022804 18.683035564931547 26-27 20.052257206394934 26.707380195312147 34.625011291169756 18.615351307123163 28-29 18.678859690375962 28.140640353593867 34.45991672497448 18.720583231055695 30-31 19.1767223745235 28.156651105172 33.540851791101275 19.12577472920323 32-33 19.106896380327516 28.316376274647 33.251606810352406 19.325120534673072 34-35 19.926303150351515 27.391538771865758 33.34421562422924 19.337942453553488 36-37 20.16516773473733 27.22361221721144 33.87836327532322 18.732856772728013 38-39 19.87130004354366 27.644966692206545 33.955642823445515 18.52809044080428 40-41 19.133246854301312 28.077374198340326 33.9191060986836 18.87027284867476 42-43 18.65688512938997 28.783873368316552 32.601072247413185 19.958169254880293 44-45 19.392096567136928 28.354791316375326 32.05233842468549 20.20077369180225 46-47 20.007092646015785 28.70356563182858 31.442837287818048 19.846504434337582 48-49 20.17397372232073 28.554297485506176 31.463243271661877 19.808485520511216 50-51 19.62695212805616 29.222131095829916 31.880361242862033 19.270555533251894 52-53 19.58363443104958 29.768192993121588 31.04943546567868 19.598737110150147 54-55 18.773729367637443 28.977548624891508 31.45128143364571 20.797440573825337 56-57 19.92148518599236 29.645125275767274 30.230640850644065 20.2027486875963 58-59 20.244636713095037 28.87397619970492 30.773631463915535 20.107755623284508 60-61 19.64784225596604 29.01284279843101 31.65180344510661 19.687511500496342 62-63 20.065534939939848 28.821752323617954 31.41562065822659 19.697092078215604 64-65 19.516510532989283 29.458245373222496 31.423278630388786 19.601965463399434 66-67 19.27778619492814 29.03212701495826 30.870018434355867 20.820068355757737 68-69 18.889996663929622 29.445027008787534 31.16917318028832 20.495803146994525 70-71 19.89792309486394 29.50074175183431 30.144134998833845 20.45720015446791 72-73 19.58248739213055 28.700156379639296 30.718466944250082 20.99888928398007 74-75 19.167737236324427 30.17773368050378 30.127263968066437 20.527265115105354 76-77 19.612788734280752 28.75920194777551 30.55945929139621 21.06855002654753 78-79 19.14356177739967 28.84751967958246 31.090681413010753 20.91823713000712 80-81 19.504555182661342 29.625892898962025 30.22928374042102 20.640268177955612 82-83 19.21218499443686 29.17479152567647 30.599700622842136 21.01332285704454 84-85 18.969556602123365 28.826453801529578 30.39326266929594 21.81072692705112 86-87 19.234325661537518 29.166776291084638 30.26437582726572 21.334522220112117 88-89 19.462867251642454 28.44718732167445 30.17696613440365 21.91297929227944 90-91 19.087922757223282 28.099995778663832 30.783518368568696 22.028563095544186 92-93 19.214335692410042 29.249349055413447 30.349359378763722 21.186955873412785 94-95 18.912433172331983 28.795663382595986 30.27306504448168 22.018838400590347 96-97 18.366434964805023 28.64251767395801 30.210137529966392 22.780909831270574 98-99 18.852397120167623 28.74622910955369 29.606221539096822 22.79515223118187 100-101 18.92820376057788 27.67492632643483 30.828671084690907 22.568198828296385 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 12200.0 1 7607.5 2 2889.0 3 2584.0 4 2035.0 5 2027.5 6 3517.5 7 6827.0 8 9677.0 9 9849.5 10 7325.5 11 4812.5 12 4185.5 13 3999.5 14 3917.0 15 3852.0 16 3807.5 17 3808.0 18 3810.0 19 3908.5 20 4154.5 21 4447.5 22 4912.5 23 5471.5 24 5936.0 25 6912.0 26 8344.5 27 9770.0 28 10747.0 29 11777.5 30 13579.5 31 15555.5 32 18167.0 33 21011.0 34 23477.5 35 25341.0 36 27848.5 37 30431.5 38 30460.0 39 29148.5 40 28466.0 41 28425.0 42 27994.5 43 28709.0 44 30631.0 45 30708.5 46 30860.0 47 39231.0 48 65624.5 49 66417.0 50 41840.5 51 31266.0 52 25068.0 53 22377.5 54 20896.0 55 19054.5 56 17280.0 57 15726.0 58 14340.0 59 12684.5 60 10810.0 61 8887.5 62 7092.0 63 5398.5 64 3834.0 65 2666.5 66 1839.5 67 1349.5 68 911.0 69 576.5 70 402.0 71 256.5 72 166.5 73 126.0 74 87.5 75 57.5 76 39.0 77 18.5 78 6.5 79 3.0 80 2.0 81 3.0 82 2.5 83 0.5 84 0.5 85 0.0 86 0.0 87 0.5 88 1.0 89 1.0 90 0.5 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.3661921749004944 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 7.64692612687015E-4 16-17 0.002580837567818676 18-19 2.867597297576307E-4 20-21 0.0 22-23 0.0 24-25 9.558657658587688E-5 26-27 4.779328829293844E-5 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.012426254956163996 36-37 0.019595248200104762 38-39 0.009128518063951242 40-41 0.005830781171738491 42-43 0.00492270869417266 44-45 0.001816144955131661 46-47 0.001720558378545784 48-49 0.003536703333677445 50-51 5.735194595152614E-4 52-53 0.0 54-55 0.0 56-57 0.0 58-59 8.124859009799536E-4 60-61 0.002055111396596353 62-63 0.0011948322073234612 64-65 6.213127478081998E-4 66-67 0.002628630856111614 68-69 0.0029631838741621835 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.08382942766581404 78-79 0.003536703333677445 80-81 9.558657658587688E-5 82-83 0.0 84-85 9.558657658587688E-5 86-87 0.08951682897267371 88-89 0.416996440355888 90-91 0.3678171467024543 92-93 0.0 94-95 0.0010514523424446458 96-97 0.0 98-99 0.0 100-101 2.867597297576307E-4 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1046172.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 39.805026609708825 #Duplication Level Percentage of deduplicated Percentage of total 1 71.60647155528876 28.502975056856272 2 14.279099893590224 11.367599024540988 3 5.3135798796842 6.345215655109289 4 2.6159267317811032 4.165081326703818 5 1.5092951076785717 3.0038765961524447 6 0.9518625533920664 2.273334855993396 7 0.6321400540244325 1.7613646170053714 8 0.46852306189359577 1.4919658356749466 9 0.35919649586997626 1.2868043468596713 >10 2.0009858030426226 14.747211752665997 >50 0.16342478500284474 4.468137367616942 >100 0.08483432321388916 5.90610872330883 >500 0.005046801086734235 1.371778409165026 >1k 0.007690363560737883 6.227720920046131 >5k 0.0014419431676383529 4.583021743702038 >10k+ 4.8064772254611767E-4 2.497803768598884 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 13968 1.3351533017515285 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 12143 1.1607077994823032 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 9429 0.9012858306282332 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 8776 0.8388677961176556 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 8706 0.8321767357566443 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 8116 0.7757806555709769 No Hit GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 6933 0.6627017354698844 No Hit GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA 5949 0.5686445441093816 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 4390 0.41962507121199955 No Hit GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT 3562 0.3404793857988935 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 3198 0.3056858719216343 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2924 0.279495149937104 No Hit TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC 2670 0.2552161594842913 No Hit GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT 2606 0.24909861858279517 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 2587 0.24728247362766353 No Hit CTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTG 2534 0.24221638506861204 No Hit ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC 2476 0.23667236362663122 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2437 0.23294448713978197 No Hit GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA 2346 0.22424610867046718 No Hit GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG 2266 0.21659918254359703 No Hit CTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCA 2254 0.2154521436245665 No Hit GCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCT 2244 0.21449627785870776 No Hit TGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCAC 2020 0.19308488470347132 No Hit TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT 1939 0.1853423720000153 No Hit GTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTG 1796 0.17167349154823489 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1782 0.1703352794760326 No Hit ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC 1739 0.16622505668283993 No Hit TATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGG 1734 0.16574712379991052 No Hit GGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGT 1721 0.16450449830429414 No Hit GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA 1637 0.15647522587108048 No Hit ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1411 0.1348726595626723 No Hit GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1380 0.1319094756885101 No Hit AAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGC 1333 0.1274169065889739 No Hit ACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCA 1298 0.12407137640846821 No Hit GATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATA 1235 0.11804942208355797 No Hit ATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTG 1175 0.11231422748840533 No Hit TGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGAT 1168 0.11164512145230422 No Hit CAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAG 1110 0.10610110001032336 No Hit CCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCAC 1103 0.10543199397422222 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 9.558657658587689E-5 0.0 10-11 0.0 0.0 0.0 9.558657658587689E-5 0.0 12-13 0.0 0.0 0.0 1.9117315317175378E-4 0.0 14-15 0.0 0.0 0.0 2.3896644146469224E-4 0.0 16-17 0.0 0.0 0.0 4.3013959463644605E-4 0.0 18-19 0.0 0.0 0.0 5.257261712223229E-4 0.0 20-21 0.0 0.0 0.0 7.168993243940767E-4 0.0 22-23 0.0 0.0 0.0 0.0011948322073234612 0.0 24-25 0.0 0.0 0.0 0.002055111396596353 0.0 26-27 0.0 0.0 0.0 0.003775669775142137 0.0 28-29 4.7793288292938446E-5 0.0 0.0 0.011709355631769919 0.0 30-31 9.558657658587689E-5 0.0 0.0 0.02733776090356079 0.0 32-33 9.558657658587689E-5 0.0 0.0 0.05701739293347556 0.0 34-35 9.558657658587689E-5 0.0 0.0 0.1013217711810295 0.0 36-37 9.558657658587689E-5 0.0 0.0 0.1642177385745365 0.0 38-39 1.4337986487881533E-4 0.0 0.0 0.2545470534481902 0.0 40-41 1.9117315317175378E-4 0.0 0.0 0.35467399242189623 0.0 42-43 1.9117315317175378E-4 0.0 0.0 0.4579552884229362 0.0 44-45 2.8675972975763066E-4 0.0 0.0 0.5849898487055666 0.0 46-47 2.8675972975763066E-4 0.0 0.0 0.7341049081795346 0.0 48-49 2.8675972975763066E-4 0.0 0.0 0.8956940158979594 0.0 50-51 2.8675972975763066E-4 0.0 0.0 1.0647866698783757 0.0 52-53 2.8675972975763066E-4 0.0 0.0 1.2215008621909207 0.0 54-55 2.8675972975763066E-4 0.0 0.0 1.397619129550399 0.0 56-57 2.8675972975763066E-4 0.0 0.0 1.616464596643764 0.0 58-59 2.8675972975763066E-4 0.0 0.0 1.8636036904065487 0.0 60-61 2.8675972975763066E-4 0.0 0.0 2.1635543677330302 0.0 62-63 2.8675972975763066E-4 0.0 0.0 2.455380186049713 0.0 64-65 2.8675972975763066E-4 0.0 0.0 2.7373127936897568 0.0 66-67 2.8675972975763066E-4 0.0 0.0 3.061638048045637 0.0 68-69 2.8675972975763066E-4 0.0 0.0 3.4345690765954355 0.0 70-71 2.8675972975763066E-4 0.0 0.0 3.866811575916771 0.0 72-73 2.8675972975763066E-4 0.0 0.0 4.30985535839231 0.0 74-75 2.8675972975763066E-4 0.0 0.0 4.780093521906531 0.0 76-77 2.8675972975763066E-4 0.0 0.0 5.246842775375368 0.0 78-79 2.8675972975763066E-4 0.0 0.0 5.801722852456384 0.0 80-81 2.8675972975763066E-4 0.0 0.0 6.400429374902024 0.0 82-83 2.8675972975763066E-4 0.0 0.0 6.988239027616874 0.0 84-85 2.8675972975763066E-4 0.0 0.0 7.65390394696092 0.0 86-87 2.8675972975763066E-4 0.0 0.0 8.34418240977583 0.0 88-89 2.8675972975763066E-4 0.0 0.0 9.039574754438085 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTGTACG 85 1.6419706E-5 33.499775 9 ACACCGT 75 3.0942683E-4 31.638676 6 GTAAGCG 160 0.0 31.144321 94-95 TTTGCGT 565 0.0 30.238735 3 CTTTGCG 625 0.0 27.335814 2 TTGCGTT 650 0.0 26.284437 4 GAGTACT 8860 0.0 23.838415 12-13 CATATAG 255 4.965841E-10 22.333183 3 AGTACTT 9085 0.0 22.098686 12-13 GTACTTT 9515 0.0 21.99788 14-15 GCTTTGC 850 0.0 21.296936 1 CTCCGAC 245 0.0 20.33915 94-95 ACTTTTT 10675 0.0 20.051134 16-17 ATTGCCG 120 0.004787646 19.774172 8 GTACATG 6290 0.0 19.464127 1 TACTTTT 10335 0.0 19.448853 14-15 CGCAGAA 295 3.0486262E-9 19.304955 9 TACATGG 6385 0.0 18.50751 2 TATAGGT 235 2.5484132E-6 18.17541 3 ATGCCGC 80 0.0011985458 17.796755 94-95 >>END_MODULE