##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938878_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1047189 Sequences flagged as poor quality 0 Sequence length 101 %GC 40 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.927121083204657 31.0 31.0 34.0 30.0 34.0 2 32.15799344721918 33.0 31.0 34.0 30.0 34.0 3 32.206799345676856 34.0 31.0 34.0 30.0 34.0 4 35.86326632537202 37.0 35.0 37.0 35.0 37.0 5 35.78892348945606 37.0 35.0 37.0 35.0 37.0 6 35.86214809361061 37.0 35.0 37.0 35.0 37.0 7 35.80148664663208 37.0 35.0 37.0 33.0 37.0 8 35.785479984988385 37.0 35.0 37.0 33.0 37.0 9 37.381088800589005 39.0 37.0 39.0 34.0 39.0 10-11 37.298315776808195 39.0 37.0 39.0 34.0 39.0 12-13 37.21570700226989 39.0 37.0 39.0 33.0 39.0 14-15 38.26600690037806 40.0 38.0 41.0 33.0 41.0 16-17 38.26232322914011 40.0 37.5 41.0 33.5 41.0 18-19 38.436783617856946 40.0 38.0 41.0 33.5 41.0 20-21 38.453999230320406 40.0 38.0 41.0 34.0 41.0 22-23 38.372066551501206 40.0 38.0 41.0 34.0 41.0 24-25 38.266329191769586 40.0 37.5 41.0 33.5 41.0 26-27 38.02278194289665 40.0 37.0 41.0 33.0 41.0 28-29 37.800266714031565 40.0 37.0 41.0 33.0 41.0 30-31 37.53940501666844 39.5 36.0 41.0 33.0 41.0 32-33 37.23087284148325 39.0 35.0 41.0 32.0 41.0 34-35 37.09791546702649 39.0 35.0 41.0 31.5 41.0 36-37 36.91408188970664 39.0 35.0 41.0 31.0 41.0 38-39 36.71069787784249 39.0 35.0 41.0 31.0 41.0 40-41 36.515888726867836 39.0 35.0 41.0 30.5 41.0 42-43 36.28059500243032 39.0 35.0 41.0 30.0 41.0 44-45 35.919625779109595 39.0 35.0 41.0 29.5 41.0 46-47 35.41377105756459 38.5 35.0 40.0 26.5 41.0 48-49 34.9544614200493 38.0 35.0 40.0 24.5 41.0 50-51 34.63483860124581 38.0 34.0 40.0 24.0 41.0 52-53 34.37118657663516 38.0 34.0 40.0 23.0 41.0 54-55 34.133435320653675 38.0 34.0 40.0 23.0 41.0 56-57 33.843333438376455 38.0 33.0 40.0 22.0 41.0 58-59 33.537773983492954 37.0 33.0 40.0 21.0 41.0 60-61 33.058657988195066 36.5 32.5 39.5 19.5 41.0 62-63 32.71337599993889 36.0 32.0 39.0 18.0 40.5 64-65 32.43036452827522 36.0 32.0 39.0 17.0 40.0 66-67 31.93673634845286 35.0 31.0 39.0 12.0 40.0 68-69 31.505872387887955 35.0 31.0 38.0 9.0 40.0 70-71 30.989082200061308 35.0 30.5 37.0 7.5 39.5 72-73 30.425344422067077 34.5 30.0 37.0 3.5 39.0 74-75 29.93941972270526 34.0 29.0 36.0 2.0 39.0 76-77 28.002573556444922 32.0 26.5 34.5 2.0 36.5 78-79 28.99104411906542 34.0 29.0 35.0 2.0 37.0 80-81 28.986370177685213 34.0 29.0 35.0 2.0 37.0 82-83 28.633189901727384 34.0 29.0 35.0 2.0 36.5 84-85 28.190005338100384 34.0 27.5 35.0 2.0 36.0 86-87 27.58742452413079 33.0 26.5 35.0 2.0 35.5 88-89 26.934410598277864 33.0 25.5 35.0 2.0 35.0 90-91 26.376080153630337 32.5 24.5 35.0 2.0 35.0 92-93 25.969152177878108 32.0 24.0 34.0 2.0 35.0 94-95 25.48906310131218 32.0 21.5 34.0 2.0 35.0 96-97 24.714619805975808 32.0 18.0 34.0 2.0 35.0 98-99 23.668326347965838 31.0 2.0 34.0 2.0 35.0 100-101 19.34928747341693 25.0 2.0 31.5 2.0 34.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.2424477540288308 1101 2 0.20930818093656 1101 3 0.2540122734795105 1101 4 0.20809090036973998 1101 5 0.24191502238538476 1101 6 0.15629655569014744 1101 7 0.1564897520554922 1101 8 0.2076908190445934 1101 9 0.3927532006928729 1101 10-11 0.3472670764399979 1101 12-13 0.32870381706971585 1101 14-15 0.5604668435349609 1101 16-17 0.4206277043541107 1101 18-19 0.3519149345519068 1101 20-21 0.28944137001342085 1101 22-23 0.22406079132577617 1101 24-25 0.2850338115423483 1101 26-27 0.3843975925786154 1101 28-29 0.2929757087714151 1101 30-31 0.33687690797754755 1101 32-33 0.35264553019037237 1101 34-35 0.5288978365155401 1101 36-37 0.6856833587039048 1101 38-39 0.7987912787394293 1101 40-41 0.847097341715461 1101 42-43 0.7748310732993247 1101 44-45 0.9022520869971942 1101 46-47 1.0614097414207038 1101 48-49 0.6913781399355514 1101 50-51 0.625833665767864 1101 52-53 1.100912300523504 1101 54-55 0.9908676705020767 1101 56-57 0.9541775598833624 1101 58-59 1.0460355432791673 1101 60-61 1.1099264987817676 1101 62-63 1.1358830330309715 1101 64-65 0.8426007834687468 1101 66-67 0.9247347954713625 1101 68-69 0.8254481813232069 1101 70-71 0.8530613866221977 1101 72-73 0.4712447740080741 1101 74-75 0.7293526118917448 1101 76-77 0.6635469604614102 1101 78-79 1.028590547549232 1101 80-81 0.8954870043765659 1101 82-83 0.7926595946036699 1101 84-85 1.1463794274367807 1101 86-87 1.2922423341996137 1101 88-89 1.2481509498159795 1101 90-91 1.285096648981984 1101 92-93 1.383394633762169 1101 94-95 1.1916384656299428 1101 96-97 1.2840423249405148 1101 98-99 1.1785757548292217 1101 100-101 1.2482384315887884 1102 1 0.11516836888679904 1102 2 0.08427115121403972 1102 3 0.07998080644419048 1102 4 0.0032753906761868734 1102 5 -0.05788583999571273 1102 6 0.0650635482183759 1102 7 0.08776258705104567 1102 8 0.15905845012980535 1102 9 0.13754023864422038 1102 10-11 0.22262091601078282 1102 12-13 0.07490004168378306 1102 14-15 0.23766229463927147 1102 16-17 0.3782430725118431 1102 18-19 0.19180252877198 1102 20-21 0.198255678298203 1102 22-23 0.21796117314336527 1102 24-25 0.1232591826624585 1102 26-27 0.13216758039995824 1102 28-29 0.01923965663356597 1102 30-31 0.1788854824155166 1102 32-33 0.13356534849786783 1102 34-35 0.38047859422594854 1102 36-37 0.4184645377302729 1102 38-39 0.3780904295244554 1102 40-41 0.19566634866058763 1102 42-43 0.2086918744579478 1102 44-45 0.2100369689958157 1102 46-47 0.29616378177487945 1102 48-49 0.3435044688911546 1102 50-51 0.5183507817284934 1102 52-53 0.32250021731360334 1102 54-55 0.5160129093026455 1102 56-57 0.6415397270273075 1102 58-59 0.31376249433409953 1102 60-61 0.05146068133007731 1102 62-63 -0.04735032005154949 1102 64-65 0.031172588215135022 1102 66-67 0.15439519177168037 1102 68-69 0.3189186069181531 1102 70-71 0.33939536180405483 1102 72-73 0.5696348039610051 1102 74-75 0.422339182445711 1102 76-77 0.1729463383620704 1102 78-79 0.34720868059291377 1102 80-81 0.6398049991759471 1102 82-83 0.70324104353557 1102 84-85 0.6140051639168149 1102 86-87 0.38023524095054384 1102 88-89 0.5474909978723304 1102 90-91 0.9478402222679172 1102 92-93 1.0802672679743388 1102 94-95 1.095155466386995 1102 96-97 0.6837884656205482 1102 98-99 1.0545323559944215 1102 100-101 1.2556875774365217 1103 1 -0.007684624549462171 1103 2 -0.10369587595771179 1103 3 -0.052401366809192496 1103 4 -0.0987748636786776 1103 5 -0.01731481941203583 1103 6 -0.001502782824275073 1103 7 0.1508198757174526 1103 8 0.07725922414748254 1103 9 0.04903935022771577 1103 10-11 0.12254120192674378 1103 12-13 0.062267489129290254 1103 14-15 0.006350480728045227 1103 16-17 -0.018316240446061727 1103 18-19 0.07519279107796706 1103 20-21 0.11627491515248067 1103 22-23 0.08594207421676714 1103 24-25 -0.038780483714838 1103 26-27 -0.014361307335029494 1103 28-29 -0.0016928967322726862 1103 30-31 0.055229263094283 1103 32-33 -0.014668365894479507 1103 34-35 0.01463557214941602 1103 36-37 -0.0796399708820914 1103 38-39 0.08482981923505406 1103 40-41 0.05436350004838886 1103 42-43 0.12281769695313471 1103 44-45 0.1743664376497165 1103 46-47 0.16740119587432645 1103 48-49 0.15535894707829812 1103 50-51 0.20231080704228788 1103 52-53 0.3095806534896042 1103 54-55 0.41742301696736206 1103 56-57 0.2889955698871134 1103 58-59 0.13300928751559837 1103 60-61 0.20193572047718789 1103 62-63 0.29382798798152976 1103 64-65 0.5278024876902023 1103 66-67 0.4972955674217694 1103 68-69 0.1475130427533884 1103 70-71 0.10831477958699054 1103 72-73 0.25955411626983604 1103 74-75 0.2529974625687075 1103 76-77 0.07938167867833101 1103 78-79 0.4081311327847459 1103 80-81 0.4289054673187067 1103 82-83 0.41895283148393503 1103 84-85 0.5234333319938642 1103 86-87 0.2211295802364006 1103 88-89 0.45894041492505977 1103 90-91 0.39683740692062486 1103 92-93 0.5269673482969282 1103 94-95 0.5169007788743158 1103 96-97 0.5378721390402568 1103 98-99 0.39907467950784437 1103 100-101 0.8081173357385509 1104 1 -0.01803833883405659 1104 2 -0.07885383143772629 1104 3 -0.001593580816056317 1104 4 -0.08897956298915233 1104 5 -0.02339357830101818 1104 6 -0.03594483048473052 1104 7 0.025150319170464286 1104 8 0.06425181854292816 1104 9 -0.13334010015199027 1104 10-11 -0.017587243014069998 1104 12-13 -0.059530955948922326 1104 14-15 0.06415364827763881 1104 16-17 0.06303402129191937 1104 18-19 -0.01734782394247958 1104 20-21 0.012168072672217534 1104 22-23 -0.028504743022018886 1104 24-25 0.020995287219783165 1104 26-27 0.04134658248967327 1104 28-29 0.009259959028220521 1104 30-31 -0.07082009320892269 1104 32-33 0.05815602922288576 1104 34-35 0.09657319862665759 1104 36-37 0.07526435294675338 1104 38-39 0.22542197318055202 1104 40-41 0.3858555733232407 1104 42-43 0.31283681452226375 1104 44-45 0.32582802880460093 1104 46-47 0.6367448783420997 1104 48-49 0.5959153782560307 1104 50-51 0.5048384872803311 1104 52-53 0.5228926840149057 1104 54-55 0.6367318599226195 1104 56-57 0.42888651730594773 1104 58-59 0.5636717878345578 1104 60-61 0.6642172865476326 1104 62-63 0.6479353211591388 1104 64-65 0.44640364691364454 1104 66-67 0.5351572849802899 1104 68-69 0.5664630877994021 1104 70-71 0.28019772275049704 1104 72-73 0.4447379020588471 1104 74-75 0.6273244711402093 1104 76-77 0.2844097563812369 1104 78-79 0.4743311135494075 1104 80-81 0.4058417100293994 1104 82-83 0.48113056599310866 1104 84-85 0.5648481891322348 1104 86-87 0.7330280098085709 1104 88-89 0.7628852291372255 1104 90-91 0.7169659107451167 1104 92-93 0.5452154017004993 1104 94-95 0.5504698759322899 1104 96-97 0.2434252629177891 1104 98-99 0.4509727950337066 1104 100-101 1.057325350200383 1105 1 0.0029833132679186747 1105 2 -0.005906121691900523 1105 3 0.07018166332875353 1105 4 0.028368743924879425 1105 5 0.05226706279898963 1105 6 0.10620972957917019 1105 7 -0.1074695186051784 1105 8 0.013942299035115013 1105 9 -0.13853774571173716 1105 10-11 0.056024673724706986 1105 12-13 -0.0037898683040324954 1105 14-15 0.027015110959382582 1105 16-17 0.060361503391128224 1105 18-19 -0.057014341634058496 1105 20-21 0.10395415713158229 1105 22-23 -0.06822451138160801 1105 24-25 0.03179756558765945 1105 26-27 -0.08219211458058595 1105 28-29 0.05055918023720096 1105 30-31 0.07197537668068321 1105 32-33 -0.03540206630501075 1105 34-35 0.21206343758902335 1105 36-37 0.2552073937500836 1105 38-39 0.23689511487487636 1105 40-41 0.32373914454125696 1105 42-43 0.410293619672359 1105 44-45 0.16807910570427254 1105 46-47 0.26507518462853596 1105 48-49 0.17434838225933902 1105 50-51 0.2741632497918616 1105 52-53 0.2680792039886626 1105 54-55 0.30101349890210827 1105 56-57 0.1542347861837854 1105 58-59 0.19643711404244613 1105 60-61 0.11158803481460922 1105 62-63 0.07457491873623923 1105 64-65 0.22347733252549773 1105 66-67 0.19527015737837772 1105 68-69 0.20244912851714858 1105 70-71 0.4474561691622547 1105 72-73 0.24931032772186867 1105 74-75 0.4487345678472252 1105 76-77 0.3915039815364736 1105 78-79 0.5413470792407651 1105 80-81 0.39057540914410893 1105 82-83 0.8443842768990351 1105 84-85 0.478819833151519 1105 86-87 0.23692719488317238 1105 88-89 0.6882981595405049 1105 90-91 0.8135844933264451 1105 92-93 0.9259838278579906 1105 94-95 0.8144638662140444 1105 96-97 0.733960432131294 1105 98-99 1.1631572135078834 1105 100-101 1.4007988152023323 1106 1 -0.06767576066785352 1106 2 0.008815685626991865 1106 3 -0.036567776551756026 1106 4 0.09269846759926281 1106 5 0.028602014255298513 1106 6 -0.1109485975306157 1106 7 0.007803072881010564 1106 8 -0.05450430290662922 1106 9 -0.023694579169543317 1106 10-11 0.028896219654505728 1106 12-13 0.09301745059067201 1106 14-15 0.028704804760728564 1106 16-17 -0.04885712991730884 1106 18-19 -0.11363775462883297 1106 20-21 -0.03967448364511483 1106 22-23 0.05255125411502348 1106 24-25 0.12093124743977768 1106 26-27 0.19656535367931127 1106 28-29 0.19988236918417357 1106 30-31 0.2629947941564126 1106 32-33 0.333590652141595 1106 34-35 0.17078729867940012 1106 36-37 0.008596116677644261 1106 38-39 0.44770075341109816 1106 40-41 0.35325744177799123 1106 42-43 0.5990258825551109 1106 44-45 0.6784833252711167 1106 46-47 0.6240230935157598 1106 48-49 0.4435044688911489 1106 50-51 0.5102536157365947 1106 52-53 0.6302431322933586 1106 54-55 0.4377993465496104 1106 56-57 0.4196520752054411 1106 58-59 0.5943039923098112 1106 60-61 0.6174272805203529 1106 62-63 0.8017640524180933 1106 64-65 0.6424327096726259 1106 66-67 0.6571026816502261 1106 68-69 0.5833163801975054 1106 70-71 0.46553402577166736 1106 72-73 0.7346398646897505 1106 74-75 1.0028047694902433 1106 76-77 0.8000971480786703 1106 78-79 0.8455386401070797 1106 80-81 0.9540509505929471 1106 82-83 0.7077197884748401 1106 84-85 0.7167632610828107 1106 86-87 0.4966826093715966 1106 88-89 0.6423543581962186 1106 90-91 0.9178554044541514 1106 92-93 0.6239160533994408 1106 94-95 1.0026453449295047 1106 96-97 1.0025890729079947 1106 98-99 0.8634139349417893 1106 100-101 0.9468313021328782 1107 1 -0.08925539071522692 1107 2 -0.18754588291199425 1107 3 -0.20829967368688074 1107 4 -0.08564106920801606 1107 5 -0.22923034156092825 1107 6 -0.12850066911494196 1107 7 -0.2863731809726673 1107 8 -0.12121984542862663 1107 9 0.006911105468397238 1107 10-11 -0.1677294664873088 1107 12-13 -0.1082627208507958 1107 14-15 -0.0631413866822399 1107 16-17 -0.1380635552124474 1107 18-19 -0.11642602818323411 1107 20-21 -0.1300520496275226 1107 22-23 -0.1357951441104035 1107 24-25 -0.26555269872158505 1107 26-27 -0.08931375377238027 1107 28-29 -0.07691616829984582 1107 30-31 -0.26581752255913216 1107 32-33 -0.0974027496179346 1107 34-35 -0.3505963579865181 1107 36-37 -0.19456483527363133 1107 38-39 -0.35515425448423343 1107 40-41 -0.148244550853029 1107 42-43 -0.18203002390401934 1107 44-45 -0.11214989903146488 1107 46-47 -0.1605984005815344 1107 48-49 -0.25712339786575455 1107 50-51 -0.1299166978208106 1107 52-53 0.001993827834382955 1107 54-55 -0.0931019430210398 1107 56-57 -0.2379194527289883 1107 58-59 -0.2626020720559339 1107 60-61 -0.3626553384735516 1107 62-63 -0.3738548850960086 1107 64-65 -0.43219179216961834 1107 66-67 -0.41989511893628517 1107 68-69 -0.21770860288392768 1107 70-71 -0.13339493505245414 1107 72-73 -0.19538165948499753 1107 74-75 -0.3855890947100704 1107 76-77 -0.6585017127379338 1107 78-79 -0.23143049291044093 1107 80-81 -0.13993308956044714 1107 82-83 -0.3041086384745526 1107 84-85 -0.33307930226781934 1107 86-87 -0.3106309104419225 1107 88-89 -0.28101065045105145 1107 90-91 -0.11902471811145432 1107 92-93 -0.06873236001510108 1107 94-95 -0.0782009593038957 1107 96-97 -0.1108701157528067 1107 98-99 -0.186194588255443 1107 100-101 0.5212175341803977 1108 1 -8.417185993607745E-4 1108 2 -0.020840134860513615 1108 3 -0.021493549199639972 1108 4 -0.016581849812844496 1108 5 0.12002600240929695 1108 6 0.12371804382408413 1108 7 0.06834297720175897 1108 8 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-1.5015066149496548 2316 78-79 -1.535199418592324 2316 80-81 -1.5570123367016357 2316 82-83 -1.9326460437394957 2316 84-85 -2.0168342355601965 2316 86-87 -2.1379943200995974 2316 88-89 -2.4111788760259216 2316 90-91 -2.5298134842215916 2316 92-93 -3.0863215736866927 2316 94-95 -3.3878959300880815 2316 96-97 -3.723673395919935 2316 98-99 -3.9329201497183526 2316 100-101 -4.340750224818972 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 1.0 3 8.0 4 25.0 5 88.0 6 225.0 7 376.0 8 625.0 9 1123.0 10 1790.0 11 2625.0 12 3733.0 13 4934.0 14 5911.0 15 8292.0 16 10862.0 17 15520.0 18 14892.0 19 9340.0 20 8087.0 21 8238.0 22 9098.0 23 9810.0 24 11263.0 25 13089.0 26 15426.0 27 17786.0 28 21767.0 29 25570.0 30 30469.0 31 37017.0 32 44976.0 33 56885.0 34 78637.0 35 115111.0 36 147838.0 37 197225.0 38 113258.0 39 5269.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.27698269606983 21.369342531168257 16.576806618300395 18.776868154461514 2 15.187516293620348 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20.29733887579033 27.323100223550856 32.910582521397764 19.468978379261053 22-23 19.57282782764143 27.27377770392928 33.16602829097708 19.987366177452206 24-25 20.148702860706138 27.73668363590527 33.4129751171947 18.7016383861939 26-27 20.031102312954012 26.754243980790477 34.6343878707664 18.580265835489104 28-29 18.67084165322592 28.129210677346688 34.407446984259764 18.792500685167628 30-31 19.15700031226455 28.20804076437014 33.49261690105607 19.14234202230925 32-33 19.144777112822993 28.353764220212398 33.212820226339275 19.28863844062533 34-35 19.935310714365865 27.411631179395307 33.37170698446607 19.281351121772758 36-37 20.108832934855428 27.191186786100214 33.92190597067451 18.77807430836985 38-39 19.8552905339552 27.579463900708983 34.0058172212096 18.559428344126218 40-41 19.058435140346138 28.0019022862769 33.993435584403926 18.94622698897304 42-43 18.670381405298038 28.80765484443935 32.57849653354724 19.94346721671537 44-45 19.405099488294077 28.306595739906136 32.0894571008806 20.198847670919186 46-47 20.03139843351611 28.659808286207035 31.476929599899155 19.831863680377698 48-49 20.158118997007218 28.518238402728834 31.577914525213284 19.745728075050657 50-51 19.636118823605244 29.172947408880496 31.915926784490768 19.275006983023495 52-53 19.530524098324182 29.800637707233363 31.08397815485075 19.584860039591707 54-55 18.71453004185491 28.928302340838187 31.515132416402388 20.842035200904515 56-57 19.966214312793586 29.579808420447502 30.25151142725907 20.20246583949984 58-59 20.280781064681918 28.842727080002977 30.77867733143873 20.09781452387637 60-61 19.643103556120252 29.067583808305557 31.506403231959766 19.782909403614422 62-63 20.029813147387912 28.914742228957714 31.363870323313176 19.6915743003412 64-65 19.51736459928876 29.485631997310897 31.361130952608317 19.635872450792025 66-67 19.317917579240536 29.039019724964916 30.804292117223902 20.83877057857064 68-69 18.87915421847478 29.491197017495193 31.135289424340858 20.494359339689172 70-71 19.84431654648779 29.434992155188795 30.261060801822786 20.45963049650063 72-73 19.622102600390186 28.65151371910897 30.759681394667055 20.966702285833787 74-75 19.1193280296107 30.23002533449072 30.07656688525185 20.57407975064673 76-77 19.62857354554515 28.684830073592156 30.614717952517644 21.071878428345048 78-79 19.134368294548548 28.839827385505384 31.08994651395307 20.935857805992995 80-81 19.515722567750426 29.595755875968905 30.255760898939926 20.63276065734075 82-83 19.234302499357806 29.139677746805976 30.612048063912056 21.01397168992417 84-85 19.016433518686693 28.847896607011727 30.373695674801777 21.761974199499804 86-87 19.275021396024883 29.08162045603416 30.271430688832467 21.37192745910849 88-89 19.515770123143177 28.454594578784924 30.217570689976753 21.812064608095145 90-91 19.14216396552178 28.169967356342763 30.731931627943737 21.955937050191718 92-93 19.30525435236619 29.284684999555953 30.362236425325325 21.04782422275253 94-95 18.931921553797835 28.92720416276336 30.21054461038074 21.930329673058065 96-97 18.374052992774406 28.63856804056225 30.341235345768947 22.646143620894392 98-99 18.84807804512843 28.781385213175465 29.634430842951943 22.73610589874416 100-101 18.954924001157387 27.9480169253438 30.678761290963312 22.418297782535497 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 12381.0 1 7584.5 2 2759.0 3 2546.5 4 2039.0 5 2036.5 6 3527.0 7 6819.0 8 9688.0 9 9854.0 10 7283.5 11 4772.5 12 4133.5 13 3908.5 14 3869.5 15 3853.5 16 3803.5 17 3848.0 18 3850.0 19 3866.0 20 4197.0 21 4613.5 22 4976.0 23 5421.0 24 5937.0 25 6892.0 26 8316.5 27 9691.0 28 10703.0 29 11902.0 30 13797.5 31 15786.5 32 18178.0 33 21057.0 34 23604.5 35 25327.0 36 27828.5 37 30535.0 38 30636.0 39 29305.5 40 28450.0 41 28272.0 42 27941.5 43 28632.0 44 30413.5 45 30633.5 46 30855.0 47 39299.0 48 66765.5 49 67399.5 50 41741.5 51 31165.5 52 25068.0 53 22398.5 54 20773.0 55 18848.5 56 17140.0 57 15790.5 58 14389.0 59 12538.5 60 10806.0 61 8930.5 62 6928.5 63 5216.5 64 3710.5 65 2595.5 66 1767.5 67 1263.0 68 872.0 69 556.0 70 372.0 71 247.0 72 162.0 73 119.5 74 81.5 75 53.0 76 32.5 77 12.5 78 5.0 79 2.0 80 1.5 81 1.5 82 0.5 83 0.0 84 0.0 85 0.5 86 0.5 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.37242560798480506 2 0.0 3 0.0 4 0.0 5 2.864812369113885E-4 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0010026843291898596 16-17 0.0023873436409282377 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0028170654962953205 36-37 0.007591752778151795 38-39 0.011984465077459752 40-41 0.002673824877839626 42-43 8.594437107341654E-4 44-45 5.252156010042123E-4 46-47 2.864812369113885E-4 48-49 2.864812369113885E-4 50-51 6.207093466413417E-4 52-53 0.0 54-55 0.0 56-57 0.0 58-59 2.864812369113885E-4 60-61 0.0021486092768354136 62-63 0.0 64-65 9.549374563712949E-5 66-67 0.0012891655661012482 68-69 0.0014801530573755072 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.11664561029575368 78-79 0.0 80-81 0.0 82-83 0.0 84-85 0.0 86-87 0.13693803124364368 88-89 0.4181193652721715 90-91 0.3190923510464682 92-93 0.0 94-95 0.0 96-97 8.116968379156007E-4 98-99 0.0 100-101 1.9098749127425898E-4 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1047189.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 39.922096858408196 #Duplication Level Percentage of deduplicated Percentage of total 1 71.61887280627528 28.591755770621386 2 14.215394284290578 11.350166949958213 3 5.385241602038924 6.449704105275818 4 2.6015073436702734 4.154305126074594 5 1.5247124447427602 3.0434858950120414 6 0.9647256608524746 2.3108322764606606 7 0.6390734055332904 1.7859205276732963 8 0.45766861393945046 1.4616872587795335 9 0.3486686344804412 1.2527624697495388 >10 1.985372037243581 14.632999475239918 >50 0.1614298710019702 4.432247962886768 >100 0.0827452589744666 5.813061806885074 >500 0.0050221110833889235 1.3876993981892218 >1k 0.0076527406984974065 6.2708546179473315 >5k 0.0014348888809682637 4.557306024985505 >10k+ 4.782962936560879E-4 2.505210334261106 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 14357 1.3710037061122682 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 11883 1.1347521794060098 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 9392 0.8968772590239202 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 8683 0.8291721933671954 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 8528 0.8143706627934403 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 8140 0.7773190894862341 No Hit GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 6941 0.6628220884673158 No Hit GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA 6050 0.5777371611046335 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 4357 0.4160662497409732 No Hit GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT 3529 0.33699742835343 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 3217 0.3072033797146456 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2919 0.278746243514781 No Hit GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT 2663 0.2542998446316759 No Hit ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC 2638 0.2519125009907476 No Hit TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC 2601 0.2483792324021738 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 2531 0.24169467020757474 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2497 0.23844788285591234 No Hit CTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTG 2497 0.23844788285591234 No Hit GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA 2355 0.22488777097543997 No Hit GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG 2333 0.22278690857142314 No Hit CTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCA 2284 0.21810771503520376 No Hit GCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCT 2120 0.20244674075071456 No Hit TGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCAC 2090 0.19958192838160066 No Hit TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT 1960 0.18716774144877382 No Hit GTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTG 1858 0.1774273793937866 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1813 0.1731301608401158 No Hit ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC 1735 0.16568164868041968 No Hit GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA 1719 0.1641537487502256 No Hit GGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGT 1694 0.16176640510929735 No Hit TATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGG 1690 0.16138443012674886 No Hit ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1484 0.14171271852550016 No Hit AAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGC 1387 0.1324498251986986 No Hit ACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCA 1365 0.13034896279468178 No Hit GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1359 0.12977600032085898 No Hit GATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATA 1287 0.12290045063498567 No Hit ATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTG 1232 0.11764829462494353 No Hit TGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGAT 1194 0.11401953229073263 No Hit GTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCA 1113 0.10628453889412513 No Hit CAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAG 1084 0.10351522027064837 No Hit CCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCAC 1077 0.10284676405118848 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 9.54937456371295E-5 0.0 10-11 0.0 0.0 0.0 1.90987491274259E-4 0.0 12-13 0.0 0.0 0.0 2.864812369113885E-4 0.0 14-15 0.0 0.0 0.0 2.864812369113885E-4 0.0 16-17 0.0 0.0 0.0 2.864812369113885E-4 0.0 18-19 4.774687281856475E-5 0.0 0.0 3.81974982548518E-4 0.0 20-21 9.54937456371295E-5 0.0 0.0 5.252156010042122E-4 0.0 22-23 9.54937456371295E-5 0.0 0.0 0.001145924947645554 0.0 24-25 1.90987491274259E-4 0.0 0.0 0.002100862404016849 0.0 26-27 1.90987491274259E-4 0.0 0.0 0.0035810154613923565 0.0 28-29 1.90987491274259E-4 0.0 0.0 0.011650236967729799 0.0 30-31 1.90987491274259E-4 0.0 0.0 0.03151293606025274 0.0 32-33 1.90987491274259E-4 0.0 0.0 0.06546096263425227 0.0 34-35 1.90987491274259E-4 0.0 0.0 0.11378079792663978 0.0 36-37 1.90987491274259E-4 0.0 0.0 0.18621280399240253 0.0 38-39 1.90987491274259E-4 0.0 0.0 0.28294796832281466 0.0 40-41 1.90987491274259E-4 0.0 0.0 0.3936252195162478 0.0 42-43 1.90987491274259E-4 0.0 0.0 0.5060213581311492 0.0 44-45 1.90987491274259E-4 0.0 0.0 0.6315956336439745 0.0 46-47 2.3873436409282376E-4 0.0 0.0 0.7866774765586728 0.0 48-49 2.864812369113885E-4 0.0 0.0 0.9488736035233372 0.0 50-51 2.864812369113885E-4 0.0 0.0 1.132985545111723 0.0 52-53 2.864812369113885E-4 0.0 0.0 1.3075958590092143 0.0 54-55 2.864812369113885E-4 0.0 0.0 1.4948590942036253 0.0 56-57 2.864812369113885E-4 0.0 0.0 1.723184640022002 0.0 58-59 2.864812369113885E-4 0.0 0.0 1.979298865820783 0.0 60-61 2.864812369113885E-4 0.0 0.0 2.282777989455581 0.0 62-63 2.864812369113885E-4 0.0 0.0 2.5789040946763193 0.0 64-65 2.864812369113885E-4 0.0 0.0 2.87063748759775 0.0 66-67 2.864812369113885E-4 0.0 0.0 3.214414971891416 0.0 68-69 2.864812369113885E-4 0.0 0.0 3.59619896694866 0.0 70-71 2.864812369113885E-4 0.0 0.0 4.027353228500299 0.0 72-73 2.864812369113885E-4 0.0 0.0 4.4786566703813735 0.0 74-75 2.864812369113885E-4 0.0 0.0 4.949584076990877 0.0 76-77 3.3422810972995326E-4 0.0 0.0 5.42122768669266 0.0 78-79 3.81974982548518E-4 0.0 0.0 5.983017392275893 0.0 80-81 3.81974982548518E-4 0.0 0.0 6.591360298857227 0.0 82-83 3.81974982548518E-4 0.0 0.0 7.200085180421109 0.0 84-85 3.81974982548518E-4 0.0 0.0 7.8679684374071925 0.0 86-87 3.81974982548518E-4 0.0 0.0 8.561444018224027 0.0 88-89 3.81974982548518E-4 0.0 0.0 9.261890642472371 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CTTTGCG 525 0.0 31.635757 2 TTTGCGT 535 0.0 31.044434 3 CGCAGAA 285 0.0 29.972147 9 TATAGCG 35 0.008433193 27.116364 66-67 TTGCGTT 635 0.0 26.155548 4 GAGTACT 8535 0.0 23.411596 12-13 GCTTTGC 755 0.0 22.713663 1 AGTACTT 8705 0.0 22.191063 12-13 CTATTAC 130 2.8851052E-4 21.98566 1 GTACTTT 9170 0.0 21.738642 14-15 GTACATG 6870 0.0 21.078926 1 TACATGG 7030 0.0 20.317987 2 ACTTTTT 10190 0.0 19.746597 16-17 ACATGGG 6790 0.0 19.708284 3 TACTTTT 10035 0.0 19.297241 14-15 CATGGGG 1470 0.0 18.723202 4 GCCTATC 130 0.0076053995 18.251398 7 CATGGGA 5040 0.0 18.17173 4 CCTACAC 315 6.886694E-9 18.077576 3 CAATACT 475 0.0 17.98243 4 >>END_MODULE