##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938877_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 815433 Sequences flagged as poor quality 0 Sequence length 101 %GC 40 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.13270372918437 33.0 31.0 34.0 31.0 34.0 2 32.208342071022386 34.0 31.0 34.0 30.0 34.0 3 32.2507195563584 34.0 31.0 34.0 30.0 34.0 4 35.724760955222564 37.0 35.0 37.0 35.0 37.0 5 35.78722715416227 37.0 35.0 37.0 35.0 37.0 6 35.91832805392963 37.0 36.0 37.0 35.0 37.0 7 35.891830475342545 37.0 36.0 37.0 35.0 37.0 8 35.90798876179895 37.0 36.0 37.0 35.0 37.0 9 37.52492356821468 39.0 37.0 39.0 35.0 39.0 10-11 37.454075932664 39.0 37.0 39.0 35.0 39.0 12-13 37.40933467249915 39.0 37.0 39.0 35.0 39.0 14-15 38.54101624535676 40.0 38.0 41.0 34.0 41.0 16-17 38.491969910464746 40.0 38.0 41.0 34.5 41.0 18-19 38.45917567721689 40.0 38.0 41.0 34.0 41.0 20-21 38.551868148578734 40.0 38.0 41.0 34.0 41.0 22-23 38.53250420328831 40.0 38.0 41.0 34.5 41.0 24-25 38.43431771831652 40.0 38.0 41.0 34.0 41.0 26-27 38.219258970387514 40.0 38.0 41.0 34.0 41.0 28-29 38.12175126589186 40.0 38.0 41.0 34.0 41.0 30-31 38.061931513686595 40.0 38.0 41.0 34.0 41.0 32-33 37.879358573911034 40.0 37.0 41.0 34.0 41.0 34-35 37.58167685143967 40.0 37.0 41.0 33.0 41.0 36-37 37.26745422370691 40.0 36.0 41.0 32.5 41.0 38-39 36.983995006334055 39.0 35.5 41.0 31.5 41.0 40-41 36.9455871910997 39.0 35.5 41.0 31.5 41.0 42-43 36.83391584593707 39.0 36.0 41.0 31.0 41.0 44-45 36.8298965089713 40.0 36.0 41.0 31.0 41.0 46-47 36.618670693974856 40.0 36.0 41.0 31.0 41.0 48-49 36.38955990253031 40.0 36.0 41.0 30.0 41.0 50-51 35.268882912513966 38.0 34.0 40.0 28.5 40.5 52-53 35.398514041006436 38.5 35.0 39.5 28.5 40.5 54-55 36.002005682870326 39.0 35.0 40.5 29.0 41.0 56-57 35.98753790930708 39.0 35.0 41.0 28.5 41.0 58-59 35.87570775281354 39.0 35.0 41.0 28.0 41.0 60-61 35.485282665773894 39.0 35.0 40.5 28.0 41.0 62-63 35.15393723334719 38.0 35.0 40.0 28.0 41.0 64-65 34.71025025967799 38.0 34.5 40.0 26.0 41.0 66-67 34.21539231304105 37.0 34.0 39.5 26.0 41.0 68-69 33.795658870808516 36.5 34.0 39.0 26.0 41.0 70-71 33.18521877333883 36.0 33.0 38.5 24.5 40.0 72-73 32.654704923641795 35.0 32.5 37.5 23.0 40.0 74-75 32.204840863688375 35.0 32.0 37.0 22.0 39.0 76-77 31.724068684980864 35.0 32.0 36.5 20.5 39.0 78-79 31.29729113244129 35.0 31.5 36.0 19.0 38.5 80-81 30.8591404811922 35.0 31.0 36.0 16.0 37.5 82-83 30.37385842858947 34.0 31.0 35.0 9.0 37.0 84-85 29.945183111304054 34.0 30.0 35.0 4.5 36.5 86-87 29.586701789110815 34.0 30.0 35.0 2.0 36.0 88-89 28.997067202332993 34.0 29.0 35.0 2.0 36.0 90-91 28.463296800595515 34.0 29.0 35.0 2.0 35.0 92-93 28.038884249227095 34.0 29.0 35.0 2.0 35.0 94-95 27.507462293039403 34.0 27.0 35.0 2.0 35.0 96-97 26.778805248254606 33.5 25.0 35.0 2.0 35.0 98-99 26.035671232339137 33.0 24.5 35.0 2.0 35.0 100-101 22.879498990107095 29.5 10.0 33.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.1645816819791861 1101 2 0.25327533264553637 1101 3 0.2922922759949387 1101 4 0.21529539851627533 1101 5 -0.006333323645343114 1101 6 0.1710263876888547 1101 7 0.25245006702675 1101 8 0.13889834307848048 1101 9 0.25194123033981697 1101 10-11 0.17342090207471728 1101 12-13 0.1850781005370692 1101 14-15 0.3288382659447677 1101 16-17 0.32124027501777874 1101 18-19 0.3700701890928144 1101 20-21 0.2902823773742753 1101 22-23 0.502120908059041 1101 24-25 0.27874311743542535 1101 26-27 0.2946202294316578 1101 28-29 0.26891616718005906 1101 30-31 0.24766534432399112 1101 32-33 0.2948993783258942 1101 34-35 0.1483007090998143 1101 36-37 0.24378222752848444 1101 38-39 0.1386602054948014 1101 40-41 -0.004583030769182983 1101 42-43 0.1637406145506759 1101 44-45 0.2786618050616809 1101 46-47 0.3463417123682646 1101 48-49 0.31579671024898914 1101 50-51 0.28335543109702854 1101 52-53 0.16467051114423015 1101 54-55 0.2783475342278692 1101 56-57 0.26117876497868764 1101 58-59 0.3323801607990404 1101 60-61 0.2128193379572778 1101 62-63 0.1975139479204202 1101 64-65 0.2732909772286831 1101 66-67 0.11344910569782485 1101 68-69 0.03152447828853866 1101 70-71 0.1747629672751856 1101 72-73 0.08931207668539543 1101 74-75 -0.019717407423314626 1101 76-77 0.2778489099384167 1101 78-79 0.13876143415238218 1101 80-81 0.21262867769747018 1101 82-83 0.039213585689083175 1101 84-85 -0.12191737072484798 1101 86-87 -0.03851390272852839 1101 88-89 0.06786397814883927 1101 90-91 0.13291031707114698 1101 92-93 0.25895213016874763 1101 94-95 -0.032321573237776846 1101 96-97 -0.5149488665993118 1101 98-99 -0.6038034691125063 1101 100-101 -0.4215910139716854 1102 1 0.08099213514289261 1102 2 0.06978942858367532 1102 3 0.21285712043529514 1102 4 0.08333748755184445 1102 5 0.21034951731247986 1102 6 0.223507477656689 1102 7 0.2558857295158816 1102 8 0.3828980861416227 1102 9 0.41403193252395454 1102 10-11 0.3150295698744614 1102 12-13 0.2829970855670254 1102 14-15 0.3659152966574055 1102 16-17 0.5278800096089427 1102 18-19 0.5188019105524262 1102 20-21 0.4989324692986443 1102 22-23 0.4975420501433945 1102 24-25 0.5187707555085979 1102 26-27 0.5184113675404305 1102 28-29 0.6824933706306524 1102 30-31 0.5692591514512131 1102 32-33 0.6202699837073311 1102 34-35 0.5692613751912603 1102 36-37 0.5201959444847546 1102 38-39 0.7664533088233512 1102 40-41 0.6270294042804778 1102 42-43 0.5150531626178321 1102 44-45 0.5919259969171264 1102 46-47 0.749542485956546 1102 48-49 0.7409890222814468 1102 50-51 0.7098558642439698 1102 52-53 0.45371840382794915 1102 54-55 0.8498523682865837 1102 56-57 0.6571045796681858 1102 58-59 0.6559608884511832 1102 60-61 0.9917390573405598 1102 62-63 0.8117788046867958 1102 64-65 0.6798761995398337 1102 66-67 0.5204683429772743 1102 68-69 0.5866617398624143 1102 70-71 0.4651346056122492 1102 72-73 0.6719693593697542 1102 74-75 0.4476958807261653 1102 76-77 0.666291438544345 1102 78-79 0.5712990155084832 1102 80-81 0.641625564942121 1102 82-83 0.6339477688815904 1102 84-85 0.5293255613413947 1102 86-87 0.494480783192202 1102 88-89 0.44988308522380294 1102 90-91 0.5810602848672382 1102 92-93 0.4900173591463606 1102 94-95 0.16460403833271542 1102 96-97 0.4856387896677816 1102 98-99 0.1929594736478606 1102 100-101 0.53996133604576 1103 1 0.1528017038962446 1103 2 0.1979250906960104 1103 3 0.17954784188946604 1103 4 0.20908087521225127 1103 5 0.1854743409920374 1103 6 -0.05176770279727805 1103 7 0.24599456164159506 1103 8 0.08167301427866391 1103 9 0.3619099730290003 1103 10-11 0.2726351597707577 1103 12-13 0.26736204778837447 1103 14-15 0.2905810413195624 1103 16-17 0.40635883996069566 1103 18-19 0.299104484185051 1103 20-21 0.2192955645469965 1103 22-23 0.15023254080938386 1103 24-25 0.36928202021059064 1103 26-27 0.3866683484521829 1103 28-29 0.22567659405255824 1103 30-31 0.2870456861667421 1103 32-33 0.3017814395845875 1103 34-35 0.2483883069658006 1103 36-37 0.3450087735516689 1103 38-39 0.6160465877205255 1103 40-41 0.5984288934463464 1103 42-43 0.5155190447195181 1103 44-45 0.7372682953951326 1103 46-47 0.8396153220159874 1103 48-49 0.6170462990843077 1103 50-51 0.573956074536774 1103 52-53 0.7152978561021683 1103 54-55 0.7797564878555292 1103 56-57 0.7484682794437703 1103 58-59 0.8496455594660759 1103 60-61 0.7988983132381478 1103 62-63 0.737544237784924 1103 64-65 0.6047890942263621 1103 66-67 0.48903551008685753 1103 68-69 0.4434912614591937 1103 70-71 0.4391528516565586 1103 72-73 0.44370349139092724 1103 74-75 0.2744566308910734 1103 76-77 0.48694835814222515 1103 78-79 0.2619894682838826 1103 80-81 0.1227379639507653 1103 82-83 0.2901190274866323 1103 84-85 -0.021601983227419908 1103 86-87 0.10224249956731057 1103 88-89 0.2514052538059559 1103 90-91 0.39995012484836323 1103 92-93 0.5258248335831261 1103 94-95 0.33865363023377526 1103 96-97 0.5221799638284601 1103 98-99 0.5376020017084322 1103 100-101 0.903381237909116 1104 1 0.2604730491907503 1104 2 0.20111246723061527 1104 3 0.1891532547161887 1104 4 0.2422825698369948 1104 5 0.18684728482253377 1104 6 0.14964990931925826 1104 7 0.326141239827372 1104 8 0.23373563298289923 1104 9 0.0887599412591129 1104 10-11 0.16190109362360516 1104 12-13 0.1511041767343002 1104 14-15 0.27258474290078283 1104 16-17 0.22998143795823722 1104 18-19 0.25743312850256217 1104 20-21 0.30866503186902605 1104 22-23 0.3753070312360052 1104 24-25 0.3365096951570479 1104 26-27 0.2787104682220516 1104 28-29 0.41968325274356744 1104 30-31 0.3513636897367505 1104 32-33 0.44092747890999817 1104 34-35 0.39080742360715703 1104 36-37 0.3529001707514112 1104 38-39 0.32118049735554877 1104 40-41 0.31581022383411295 1104 42-43 0.1946613790010474 1104 44-45 0.5523580388295031 1104 46-47 0.7399409849851182 1104 48-49 0.6488135877020298 1104 50-51 0.32918685202465525 1104 52-53 0.4853124512049263 1104 54-55 0.5747212639687191 1104 56-57 0.6946732930260495 1104 58-59 0.5625765088749191 1104 60-61 0.5002683138094923 1104 62-63 0.7081376161443487 1104 64-65 0.45400939024897724 1104 66-67 0.7584527967135628 1104 68-69 0.6681138750386921 1104 70-71 0.5158983341865877 1104 72-73 0.5585762993121008 1104 74-75 0.41655978000523675 1104 76-77 0.6311602658762432 1104 78-79 0.8262676512888127 1104 80-81 0.7321640111017764 1104 82-83 0.7939591150226235 1104 84-85 0.9486953378873473 1104 86-87 0.9275479492713394 1104 88-89 0.863252564446185 1104 90-91 1.0769447456094206 1104 92-93 1.0250665115178599 1104 94-95 0.7896397086179583 1104 96-97 0.7671127969231577 1104 98-99 1.2855646604063686 1104 100-101 1.0004378056187946 1105 1 0.11073306934630978 1105 2 0.1473267386442103 1105 3 0.2516994164956685 1105 4 0.2774000121337181 1105 5 0.2794080669523922 1105 6 0.22486816305384139 1105 7 0.19322223387660387 1105 8 0.1947502752049246 1105 9 0.35440110839714123 1105 10-11 0.3092671592688063 1105 12-13 0.39391219252225085 1105 14-15 0.35838466449936845 1105 16-17 0.34802099870839953 1105 18-19 0.34649528938698637 1105 20-21 0.27944872639287155 1105 22-23 0.24631734960296825 1105 24-25 0.393861342343925 1105 26-27 0.1989959099534815 1105 28-29 0.29619344745163545 1105 30-31 0.5022022989023114 1105 32-33 0.36290797277163733 1105 34-35 0.3632037310012919 1105 36-37 0.31280188296436506 1105 38-39 0.3643088485535202 1105 40-41 0.35344879329423406 1105 42-43 0.36149320459322354 1105 44-45 0.4155088673440659 1105 46-47 0.40933431615371774 1105 48-49 0.2627984828106946 1105 50-51 0.3097934367343598 1105 52-53 0.2671731221259108 1105 54-55 0.3822979366865482 1105 56-57 0.34003810357557995 1105 58-59 0.204429238708002 1105 60-61 0.5668052087431761 1105 62-63 0.5668939273744229 1105 64-65 0.46422681117878994 1105 66-67 0.4379954102399921 1105 68-69 0.662471833035049 1105 70-71 0.4103230848078212 1105 72-73 0.3992565409769 1105 74-75 0.3491984924722473 1105 76-77 0.381843754638421 1105 78-79 0.5172255786648741 1105 80-81 0.5694272295641305 1105 82-83 0.7805896542258566 1105 84-85 0.34391167364731245 1105 86-87 0.42350421945003447 1105 88-89 0.5471838067385342 1105 90-91 0.7298670491305863 1105 92-93 0.7612272310611807 1105 94-95 0.7086263113970013 1105 96-97 0.8915514949212309 1105 98-99 1.40801303912896 1105 100-101 1.205518924168132 1106 1 0.12676878680377968 1106 2 0.2026242091958821 1106 3 0.08657076421609844 1106 4 0.23437436241346887 1106 5 0.24623055344935096 1106 6 0.20146127953118054 1106 7 0.12351506212762331 1106 8 0.13343476830605994 1106 9 0.21438124742395814 1106 10-11 0.1676826411396135 1106 12-13 0.2879530932093175 1106 14-15 0.26085679051158905 1106 16-17 0.2933847532396854 1106 18-19 0.2822870236697739 1106 20-21 0.35140842314972076 1106 22-23 0.40113605767088245 1106 24-25 0.3828667597069426 1106 26-27 0.32940822698628125 1106 28-29 0.4185548041337199 1106 30-31 0.31803859927126865 1106 32-33 0.48362635870425663 1106 34-35 0.5297928914311285 1106 36-37 0.44601572751592755 1106 38-39 0.6126194547976169 1106 40-41 0.7587547410905557 1106 42-43 0.6260123026143916 1106 44-45 0.6086963920693549 1106 46-47 0.4486388591494901 1106 48-49 0.6652770676759872 1106 50-51 0.6031303224368756 1106 52-53 0.5527827974878079 1106 54-55 0.7057960515088055 1106 56-57 0.5418159028828669 1106 58-59 0.6713897831893192 1106 60-61 0.784751939352482 1106 62-63 0.6325933807939847 1106 64-65 0.6495046629642616 1106 66-67 0.866285994039572 1106 68-69 0.7973084644202331 1106 70-71 0.6844691033285812 1106 72-73 0.6444628609646301 1106 74-75 0.4694542630537839 1106 76-77 0.3796695432240149 1106 78-79 0.489805325316361 1106 80-81 0.5956307964657377 1106 82-83 0.7753055050586468 1106 84-85 0.7678740185339201 1106 86-87 0.638491180640397 1106 88-89 0.5815731625486755 1106 90-91 0.6992754989251146 1106 92-93 0.5916556952266561 1106 94-95 0.8076033905421731 1106 96-97 0.9845662453269242 1106 98-99 0.9393691560664976 1106 100-101 0.9136659599582515 1107 1 -0.12111518058966908 1107 2 0.0308984766927054 1107 3 0.007895227809918026 1107 4 0.02365965395486569 1107 5 -0.1792470630843468 1107 6 -0.14142359535340887 1107 7 -0.22910443078210818 1107 8 -0.03105155225137679 1107 9 -0.0898965869076136 1107 10-11 -0.07358058653018418 1107 12-13 0.009480361345332255 1107 14-15 -0.1678562292243626 1107 16-17 -0.18482441163171615 1107 18-19 -0.010140554591473006 1107 20-21 0.05985519287582264 1107 22-23 0.01729097824568271 1107 24-25 0.09152974906167799 1107 26-27 0.005473345394150897 1107 28-29 0.04125234128167676 1107 30-31 0.15258007950769326 1107 32-33 0.08800835723648959 1107 34-35 -0.07540269263806465 1107 36-37 -0.0044589298075266015 1107 38-39 -0.03583820482217703 1107 40-41 0.059842152215608735 1107 42-43 0.16868174699307303 1107 44-45 -3.85621456210572E-4 1107 46-47 0.15537007605565378 1107 48-49 0.07282581573277014 1107 50-51 0.1987246502226796 1107 52-53 0.17135085537785244 1107 54-55 0.28233385443329695 1107 56-57 0.22989383664390317 1107 58-59 0.24459590993820512 1107 60-61 0.2269226581643835 1107 62-63 0.194572658431035 1107 64-65 0.3819123553326591 1107 66-67 0.38105048036013045 1107 68-69 0.42874474670521323 1107 70-71 0.09939378156379064 1107 72-73 0.21198414755033212 1107 74-75 0.302057657859784 1107 76-77 0.17292648947686828 1107 78-79 0.2744884530832259 1107 80-81 0.07687492736541657 1107 82-83 0.3495501173231901 1107 84-85 0.6236247504452557 1107 86-87 0.7677218648887205 1107 88-89 0.6465495283228435 1107 90-91 0.6667581184969329 1107 92-93 0.28008163211261916 1107 94-95 0.014332240080140224 1107 96-97 0.4197855819903573 1107 98-99 0.611801153415044 1107 100-101 0.6756870611001133 1108 1 0.22418515840649889 1108 2 0.19022828997064778 1108 3 0.06598904362952851 1108 4 0.11546278458934722 1108 5 0.2293900819937562 1108 6 0.2276484066592772 1108 7 0.25888878284353467 1108 8 0.3015743295095987 1108 9 0.2938544976543511 1108 10-11 0.30834811138262097 1108 12-13 0.23302188756995434 1108 14-15 0.4238598139065246 1108 16-17 0.26109424863992814 1108 18-19 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-1.7881121319422348 2316 100-101 -1.916793003605342 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 2858.0 3 501.0 4 437.0 5 446.0 6 478.0 7 589.0 8 683.0 9 725.0 10 976.0 11 1463.0 12 2204.0 13 3948.0 14 3280.0 15 3375.0 16 4486.0 17 6550.0 18 5620.0 19 3791.0 20 3610.0 21 3828.0 22 4407.0 23 4912.0 24 5828.0 25 6976.0 26 8099.0 27 9570.0 28 11798.0 29 14182.0 30 17892.0 31 22123.0 32 28295.0 33 37234.0 34 52658.0 35 86721.0 36 111014.0 37 165084.0 38 154428.0 39 24356.0 40 8.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 45.14558867125659 20.88503347721421 15.807420218687456 18.161957632841744 2 13.344100775403103 25.24723797928822 37.87533658483901 23.53332466046966 3 23.267191838157498 27.458458018602755 32.634850340854136 16.63949980238561 4 10.869882762777213 18.177722840943545 42.71664827482929 28.235746121449957 5 10.507798353783167 41.32323424523843 36.15784530963239 12.011122091346007 6 24.466445645798927 39.53087461932795 18.239129527880777 17.763550206992342 7 22.307280789797225 37.14432253488983 23.276367678780456 17.27202899653249 8 21.292022194246282 30.724822266885564 22.19412358003696 25.789031958831192 9 21.27555665718556 23.037496594899835 26.63610461449358 29.05084213342103 10-11 22.475677347079255 25.820970059424695 29.122335367327583 22.581017226168463 12-13 28.9311768361627 25.25250170399923 27.277177944145635 18.539143515692437 14-15 23.607866425851036 27.85696582119439 31.002763163060976 17.532404589893595 16-17 17.38656971707767 30.703845672483475 32.18152526276267 19.72805934767618 18-19 16.827630720788875 34.04191875778822 31.240954016493372 17.889496504929543 20-21 19.885130603963184 29.72388273529298 28.128890309604987 22.262096351138847 22-23 18.128766931632835 29.126887991350248 29.45149916361999 23.29284591339693 24-25 21.807270542562872 29.408649364501045 29.316658351268206 19.467421741667877 26-27 21.899256559185314 26.04914748233446 33.55197795757815 18.49961800090207 28-29 17.851037116765074 30.797538558210718 32.48707976244681 18.864344562577394 30-31 18.622139043995826 30.36718414432104 30.560409891391053 20.45026692029208 32-33 19.053433353417525 30.343856876217167 30.585848867902072 20.016860902463236 34-35 21.48278076129232 27.84746600802474 29.93341828764579 20.73633494303715 36-37 21.143723848341324 27.32857572337521 33.07534881199391 18.452351616289558 38-39 21.469053211944882 27.90489130100058 32.55211380721803 18.073941679836516 40-41 18.86163422092661 29.61608234776858 32.97913349122075 18.543149940084067 42-43 17.673339078296788 31.23568250597031 29.53769470097958 21.553283714753324 44-45 19.29239988714472 29.567712549235576 29.165758033854118 21.97412952976558 46-47 21.080711480711482 30.054115654115655 28.313729113729114 20.551443751443752 48-49 20.671289051429472 28.75881945591073 29.975429975429975 20.594461517229824 50-51 19.07205697528278 30.06561275536608 32.50917962492915 18.35315064442199 52-53 19.051984376347665 31.44290836506118 30.10713537623677 19.39797188235439 54-55 17.149411607439315 28.555165614194607 32.09266682313603 22.20275595523005 56-57 20.72540710995048 29.810302776477542 28.51568081594442 20.948609297627552 58-59 22.00846832445161 28.335942224252957 29.143241043047315 20.51234840824812 60-61 19.665054334645276 28.4006396801107 32.30883154967309 19.62547443557094 62-63 21.053649546463053 28.30426582205189 31.084743591165054 19.557341040320008 64-65 19.876369936441357 30.738145290277423 30.741841993064984 18.643642780216233 66-67 19.183177010355397 28.70624712709545 29.78639762108099 22.324178241468168 68-69 17.589972990077875 30.36918490180479 31.037993217631694 21.00284889048564 70-71 21.03420630514102 29.763349234118675 28.043451602228537 21.15899285851177 72-73 19.537415401905644 27.163443764756916 31.15612877148533 22.14301206185211 74-75 18.522484797660542 31.7399708307326 29.44919131379391 20.288353057812948 76-77 20.725022823286775 27.40389887035511 29.826062530261645 22.04501577609647 78-79 19.33515763722467 27.67312563861414 32.16478108045352 20.82693564370766 80-81 20.57802158003077 29.604727453253325 29.779469282517564 20.037781684198336 82-83 19.213388739886927 28.625028797691748 31.221502605036118 20.940079857385207 84-85 19.206046191592915 28.283218417993687 30.374609839167803 22.136125551245588 86-87 20.005100371994523 29.152753061301173 30.273479728793262 20.56866683791104 88-89 20.872641654725808 27.432516180222983 29.4701831725681 22.22465899248311 90-91 19.783559652019616 26.32279370086502 31.559812208886807 22.333834438228553 92-93 20.551780569378003 29.505583611219965 30.622613933519165 19.320021885882866 94-95 19.621337740337957 28.967983283945554 29.945879863525814 21.46479911219068 96-97 17.474443820768172 28.133947589422753 30.904507906072297 23.487100683736774 98-99 20.033765049476887 28.77010191129897 28.4789091670877 22.71722387213644 100-101 20.5281959762188 26.781510984729245 30.22012580553552 22.470167233516438 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 16368.0 1 9115.5 2 1668.0 3 1271.5 4 876.5 5 877.0 6 1593.5 7 3045.0 8 4138.0 9 3944.5 10 2789.0 11 1791.5 12 1552.5 13 1495.5 14 1411.5 15 1436.5 16 1575.0 17 1475.0 18 1301.5 19 1286.0 20 1431.0 21 1693.5 22 1924.5 23 2220.0 24 2610.5 25 3342.5 26 4657.0 27 5900.5 28 6498.0 29 7562.5 30 9497.0 31 11138.0 32 13479.5 33 16670.5 34 19953.5 35 22202.0 36 24587.0 37 27668.0 38 27371.0 39 25348.5 40 23957.0 41 22284.5 42 20323.0 43 19295.5 44 19534.0 45 19844.0 46 24445.5 47 46741.5 48 113365.0 49 110889.5 50 43307.0 51 20294.0 52 11375.5 53 7878.0 54 5945.0 55 4646.5 56 3522.5 57 2649.0 58 2091.5 59 1661.0 60 1234.0 61 893.5 62 655.0 63 507.0 64 345.5 65 225.5 66 166.0 67 135.0 68 115.5 69 101.0 70 84.0 71 73.0 72 59.0 73 40.5 74 37.5 75 30.0 76 22.0 77 16.5 78 12.0 79 10.5 80 11.0 81 9.0 82 4.0 83 1.5 84 2.0 85 4.0 86 3.0 87 0.5 88 0.0 89 0.5 90 1.0 91 0.5 92 0.0 93 0.0 94 0.0 95 0.5 96 0.5 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.3056045070533079 2 0.0773822006222461 3 0.08768347614089693 4 0.09320201659731701 5 0.10276748672177849 6 0.052364817219808374 7 0.05383642800818706 8 0.05481750186710619 9 0.05874179730278269 10-11 0.05543067302893064 12-13 0.053775110892004624 14-15 0.05211954875507859 16-17 0.05383642800818706 18-19 0.06125579906626295 20-21 0.06223687292518208 22-23 0.07505215020731318 24-25 0.0833299608919433 26-27 0.07854722582971256 28-29 0.07676902946042163 30-31 0.07149575746873134 32-33 0.06499614315339212 34-35 0.3000859665968878 36-37 0.46858540186624775 38-39 0.4419737734430665 40-41 0.42455971244725194 42-43 0.4546664164928327 44-45 0.46282159294509784 46-47 0.45932651732269847 48-49 0.47520765041395185 50-51 0.4725096973019242 52-53 0.47128335497827534 54-55 0.4626376415965506 56-57 0.44307748153435045 58-59 0.42505024937671154 60-61 0.3865431004141358 62-63 0.45399192821482576 64-65 0.47815087199070927 66-67 0.48710317095334627 68-69 0.49844683744709867 70-71 0.49636205549689555 72-73 0.4842825836089538 74-75 0.4859381457458798 76-77 0.4613499821567192 78-79 0.44350670134762754 80-81 0.4493318273849599 82-83 0.45816149211523205 84-85 0.45969442001979316 86-87 0.4571191011401305 88-89 0.46527427759239565 90-91 0.47741506659651994 92-93 0.48452785207368354 94-95 0.490230343878651 96-97 0.48274965570439266 98-99 0.4835467782147644 100-101 0.46398661815256437 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 815433.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 28.497381777967632 #Duplication Level Percentage of deduplicated Percentage of total 1 76.29667930871338 21.74255598651569 2 12.143440075334517 6.921124958493596 3 4.197547403451156 3.58857332661793 4 1.9979877801940598 2.2774968223961682 5 1.0309095123626049 1.4689110976167798 6 0.6585892102853298 1.1260840896210753 7 0.4417380812383304 0.881186512284091 8 0.35381989585216966 0.8066352522192021 9 0.27518448415597196 0.7057833573929237 >10 2.120180059869595 12.56100466789066 >50 0.2815715474310068 5.652662361465358 >100 0.16963178850656901 8.663890272675461 >500 0.00861075068561264 1.7847994306678456 >1k 0.015499351234102754 10.646591778112484 >5k 0.006027525479928848 10.690760871055389 >10k+ 0.0025832252056837922 10.481939214975325 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 21639 2.6536821541438718 No Hit GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 16021 1.9647230367179156 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 14164 1.7369912672163133 No Hit GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA 12864 1.5775667651419554 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 10624 1.3028660846445999 No Hit GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT 10121 1.2411810657650597 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 9471 1.1614688147278807 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 9021 1.1062834101636798 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 7309 0.8963336043549869 No Hit GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT 6310 0.773822006222461 No Hit ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC 6276 0.7696524423220547 No Hit TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC 6000 0.7358053941893448 No Hit GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA 5776 0.7083353261396093 No Hit GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG 5549 0.680497355392779 No Hit CTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTG 5470 0.6708092510359527 No Hit GCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCT 5267 0.6459145018658798 No Hit CTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCA 5209 0.6388017163887162 No Hit ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC 5196 0.6372074713679726 No Hit TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT 5169 0.6338963470941206 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5112 0.6269061958493217 No Hit TGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCAC 4378 0.5368926692934919 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4343 0.5326004711607207 No Hit GTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTG 4303 0.5276951018661251 No Hit GGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGT 4101 0.5029229869284172 No Hit GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA 4032 0.4944612248952397 No Hit CCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCAC 3894 0.4775377008288848 No Hit GATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATA 3717 0.4558314417002991 No Hit TATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGG 3621 0.4440585553932696 No Hit AAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGC 3431 0.42075805124394033 No Hit TGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGAT 3110 0.3813924626548104 No Hit ACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCA 3076 0.3772228987544041 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2855 0.3501207334017632 No Hit GTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTG 2819 0.3457059010366272 No Hit ATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTG 2724 0.33405564896196255 No Hit TACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACC 2682 0.3289050112026371 No Hit GTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCA 2559 0.31382100062175555 No Hit CAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAG 2457 0.3013123089205367 No Hit ACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGT 2316 0.2840208821570871 No Hit ATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATAC 2252 0.27617229128573406 No Hit GCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAG 2210 0.27102165352640867 No Hit GATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTT 1888 0.23153343070491383 No Hit CAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGT 1843 0.22601489024849375 No Hit ACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTG 1838 0.22540171908666928 No Hit CCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATA 1762 0.21608151742693757 No Hit GTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGC 1561 0.19143203672159453 No Hit GCTTTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC 1491 0.1828476404560522 No Hit TGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGT 1484 0.18198920082949796 No Hit CTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCT 1339 0.16420723713658877 No Hit ATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGT 1139 0.13968039066361063 No Hit AAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTAC 1137 0.13943512219888085 No Hit AGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAG 1117 0.13698243755158301 No Hit AACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACG 1112 0.13636926638975858 No Hit GCTTTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGT 1078 0.13219970248935228 No Hit CACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACT 1065 0.1306054574686087 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1032 0.1265585278005673 No Hit GCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAAC 1009 0.12373794045617481 No Hit GTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTT 984 0.12067208464705255 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 965 0.11834203423211963 No Hit ATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCA 878 0.10767285601637412 No Hit GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA 864 0.10595597676326565 No Hit AGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTA 858 0.1052201713690763 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 837 0.1026448524894136 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 6.13171161824454E-5 0.0 18-19 0.0 0.0 0.0 1.226342323648908E-4 0.0 20-21 0.0 0.0 0.0 6.13171161824454E-4 0.0 22-23 0.0 0.0 0.0 0.001962147717838253 0.0 24-25 0.0 0.0 0.0 0.0031884900414871605 0.0 26-27 0.0 0.0 0.0 0.00680619989625144 0.0 28-29 0.0 0.0 0.0 0.0199893798754772 0.0 30-31 0.0 0.0 0.0 0.051383743360889245 0.0 32-33 0.0 0.0 0.0 0.10350329211596783 0.0 34-35 0.0 0.0 0.0 0.17726778288344963 0.0 36-37 0.0 0.0 0.0 0.28034185518614035 0.0 38-39 0.0 0.0 0.0 0.409843604563465 0.0 40-41 0.0 0.0 0.0 0.5416754043557226 0.0 42-43 0.0 0.0 0.0 0.6848508706417327 0.0 44-45 0.0 0.0 0.0 0.8561095761393027 0.0 46-47 0.0 0.0 0.0 1.0676536269687391 0.0 48-49 0.0 0.0 0.0 1.283612510163312 0.0 50-51 0.0 0.0 0.0 1.4972413429429516 0.0 52-53 0.0 0.0 0.0 1.6886733796645463 0.0 54-55 0.0 0.0 0.0 1.9165277833985135 0.0 56-57 0.0 0.0 0.0 2.199138371883404 0.0 58-59 0.0 0.0 0.0 2.503455219496881 0.0 60-61 0.0 0.0 0.0 2.901893840450411 0.0 62-63 0.0 0.0 0.0 3.269980488893631 0.0 64-65 0.0 0.0 0.0 3.608757555801642 0.0 66-67 0.0 0.0 0.0 4.017252183809092 0.0 68-69 0.0 0.0 0.0 4.480993533496927 0.0 70-71 0.0 0.0 0.0 5.0018824354668014 0.0 72-73 0.0 0.0 0.0 5.53344051565242 0.0 74-75 0.0 0.0 0.0 6.144894798223765 0.0 76-77 0.0 0.0 0.0 6.6783537090110405 0.0 78-79 0.0 0.0 0.0 7.394660260254367 0.0 80-81 0.0 0.0 0.0 8.133470193136652 0.0 82-83 0.0 0.0 0.0 8.788398311081352 0.0 84-85 0.0 0.0 0.0 9.673633517407318 0.0 86-87 0.0 0.0 0.0 10.61877554624353 0.0 88-89 0.0 0.0 0.0 11.479054686283238 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTACCCG 25 0.0050433306 56.935413 1 GTAAGCG 100 0.0 38.055202 94-95 CATTCCG 75 7.077475E-6 37.875443 9 GCGTGTT 30 0.003923448 31.712667 92-93 GGGCGTA 75 3.1375056E-4 31.562868 7 TGTATCG 155 1.4042598E-9 30.544712 9 TAGGACG 125 2.2106906E-7 30.307789 4 GACGTGG 80 4.5797165E-4 29.590189 7 CGCAGAA 290 0.0 29.386122 9 CTTTGCG 1095 0.0 27.245874 2 CTGCCGC 90 6.5992936E-9 26.427223 94-95 AAGGGCG 90 9.1053575E-4 26.308847 5 ACGTGGA 90 9.118414E-4 26.302391 8 TTTGCGT 1155 0.0 25.830503 3 GCTTTGC 1190 0.0 25.517271 1 GTATGGT 115 1.2912031E-4 24.754528 1 TTGCGTT 1235 0.0 24.157272 4 ATTCCGC 140 1.8244275E-5 23.672152 8 TATTCCG 145 2.3932338E-5 22.855873 7 CCGTGCA 125 2.3058108E-4 22.725264 8 >>END_MODULE