##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938877_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 815433 Sequences flagged as poor quality 0 Sequence length 101 %GC 40 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.976838072533244 33.0 31.0 34.0 30.0 34.0 2 32.22733443458874 34.0 31.0 34.0 30.0 34.0 3 32.30188010541639 34.0 31.0 34.0 30.0 34.0 4 35.83667695567876 37.0 35.0 37.0 35.0 37.0 5 35.774595828228684 37.0 35.0 37.0 35.0 37.0 6 35.86286794868493 37.0 35.0 37.0 35.0 37.0 7 35.82376234466842 37.0 35.0 37.0 33.0 37.0 8 35.82905155911031 37.0 35.0 37.0 33.0 37.0 9 37.40581507003028 39.0 37.0 39.0 34.0 39.0 10-11 37.3048466274973 39.0 37.0 39.0 33.5 39.0 12-13 37.245465905843886 39.0 37.0 39.0 33.5 39.0 14-15 38.30192302739771 40.0 37.5 41.0 33.5 41.0 16-17 38.4437384800468 40.0 38.0 41.0 34.0 41.0 18-19 38.539784997663816 40.0 38.0 41.0 34.0 41.0 20-21 38.505326004711605 40.0 38.0 41.0 34.0 41.0 22-23 38.44769527355405 40.0 38.0 41.0 34.0 41.0 24-25 38.30090148424212 40.0 37.5 41.0 33.5 41.0 26-27 37.99254690452803 40.0 37.0 41.0 33.0 41.0 28-29 37.837481436243074 40.0 37.0 41.0 33.0 41.0 30-31 37.751072129776446 40.0 37.0 41.0 33.0 41.0 32-33 37.63346283508271 40.0 36.0 41.0 33.0 41.0 34-35 37.49968053782469 40.0 36.0 41.0 32.5 41.0 36-37 37.295993049091706 39.5 35.5 41.0 31.5 41.0 38-39 37.07027186782973 39.0 35.0 41.0 31.0 41.0 40-41 36.9771471107988 39.0 35.0 41.0 31.0 41.0 42-43 36.86083099408535 39.0 35.0 41.0 31.0 41.0 44-45 36.6888395490494 39.0 35.0 41.0 30.5 41.0 46-47 36.386868694301064 39.0 35.0 41.0 30.0 41.0 48-49 36.05143954193662 39.0 35.0 40.0 29.0 41.0 50-51 35.81398226463732 39.0 35.0 40.0 28.0 41.0 52-53 35.51815170590349 38.0 35.0 40.0 28.0 41.0 54-55 35.327189358291854 38.0 34.0 40.0 27.0 41.0 56-57 35.257805362304445 38.0 34.5 40.0 27.5 41.0 58-59 35.04633734469907 38.0 34.0 40.0 26.5 41.0 60-61 34.57302807220213 38.0 34.0 40.0 26.0 41.0 62-63 34.11831444152983 37.0 33.0 40.0 25.5 41.0 64-65 33.606946248189615 36.5 32.5 39.0 23.0 40.5 66-67 33.06831339913886 36.0 32.0 39.0 22.0 40.0 68-69 32.5649703899646 35.5 31.5 38.5 22.0 40.0 70-71 31.889886722759567 35.0 31.0 37.5 20.5 39.0 72-73 31.266392824425797 34.5 30.5 37.0 19.0 39.0 74-75 30.734912003806567 34.0 30.0 36.5 17.0 39.0 76-77 28.754047849424786 31.5 27.0 34.5 11.5 36.0 78-79 29.724412674002647 34.0 29.0 35.0 11.0 37.0 80-81 29.72186310831178 34.0 29.0 35.0 7.5 37.0 82-83 29.272972150992175 34.0 29.0 35.0 4.5 36.0 84-85 28.772603635123915 33.5 29.0 35.0 2.0 36.0 86-87 28.08733826077679 33.0 27.5 35.0 2.0 35.5 88-89 27.198200833176976 32.5 25.5 34.5 2.0 35.0 90-91 26.453370172656737 32.0 25.0 34.0 2.0 35.0 92-93 26.052331092805908 32.0 24.0 34.0 2.0 35.0 94-95 25.412355766813462 31.5 21.5 34.0 2.0 35.0 96-97 24.299596042838587 31.0 12.5 34.0 2.0 35.0 98-99 22.933127553091424 31.0 2.0 34.0 2.0 35.0 100-101 17.30766844118401 23.5 2.0 30.0 2.0 32.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.1458853723995901 1101 2 0.16006188752149342 1101 3 0.1835212422129473 1101 4 0.06545209608125191 1101 5 0.001404958512999599 1101 6 0.10002656612012828 1101 7 0.11701232363655123 1101 8 0.12835143535207294 1101 9 0.31338503718531285 1101 10-11 0.320094331430532 1101 12-13 0.20431556662708772 1101 14-15 0.3325871178588997 1101 16-17 0.40806198048459663 1101 18-19 0.28932067283199814 1101 20-21 0.3269898057446383 1101 22-23 0.4265270171597777 1101 24-25 0.3288413478317409 1101 26-27 0.17415593217361902 1101 28-29 0.08561571916110466 1101 30-31 0.16390766238635024 1101 32-33 0.4319139994034842 1101 34-35 0.5673463873529414 1101 36-37 0.5241767519303338 1101 38-39 0.33330895611432965 1101 40-41 0.5454614415870793 1101 42-43 0.5745715155420044 1101 44-45 0.6983390427708116 1101 46-47 0.7965718146174297 1101 48-49 0.5159830256952631 1101 50-51 0.44403227227464726 1101 52-53 0.4940133739308692 1101 54-55 0.5838563755383817 1101 56-57 0.36584528956439755 1101 58-59 0.4187732811712195 1101 60-61 0.6642274003398114 1101 62-63 0.7774003610798275 1101 64-65 0.678446763011678 1101 66-67 0.4895577259466677 1101 68-69 0.48371679858068006 1101 70-71 0.5925242868857197 1101 72-73 0.6016301558162489 1101 74-75 0.4386936424805974 1101 76-77 0.43926892120495964 1101 78-79 0.8028065811500191 1101 80-81 0.8755348548944148 1101 82-83 0.8478344228968737 1101 84-85 1.1133738397054351 1101 86-87 1.1840377895587864 1101 88-89 1.0738438344091144 1101 90-91 1.174887244725042 1101 92-93 0.9172723398085765 1101 94-95 1.0009486447272984 1101 96-97 0.7877244283599616 1101 98-99 1.2027016833657065 1101 100-101 1.5622674198825024 1102 1 0.07225907317578617 1102 2 0.12016028739510887 1102 3 0.19526915937511546 1102 4 0.09968546072153117 1102 5 0.10743307921082845 1102 6 0.035226395291836354 1102 7 0.09834447181967931 1102 8 0.2307129975976352 1102 9 0.15702159652296643 1102 10-11 0.26329218843822133 1102 12-13 0.24506610003148666 1102 14-15 0.3654819803369236 1102 16-17 0.323693810795163 1102 18-19 0.23893500713116111 1102 20-21 0.2635246649588652 1102 22-23 0.3325046185152942 1102 24-25 0.3703080059338433 1102 26-27 0.5014553000742197 1102 28-29 0.3539602298265905 1102 30-31 0.13485122828804919 1102 32-33 0.26101886957224707 1102 34-35 0.39886438074550057 1102 36-37 0.31558915816140853 1102 38-39 0.3271520197739122 1102 40-41 0.40840993095457634 1102 42-43 0.313427313200755 1102 44-45 0.1171174816016105 1102 46-47 0.34974212035493224 1102 48-49 0.39461681601741816 1102 50-51 0.369846961771664 1102 52-53 0.40258030858731786 1102 54-55 0.4262933175036423 1102 56-57 0.5873439606105677 1102 58-59 0.49254714548424516 1102 60-61 0.4778761294571723 1102 62-63 0.6057604615629799 1102 64-65 0.40639055633722165 1102 66-67 0.18675850300706287 1102 68-69 0.38615283779480336 1102 70-71 0.3880928204533092 1102 72-73 0.5580810938962024 1102 74-75 0.5701055626149021 1102 76-77 0.35231055018465796 1102 78-79 0.2948571629904855 1102 80-81 0.5394050184486154 1102 82-83 0.42794316968733526 1102 84-85 0.5305438190636842 1102 86-87 0.8934100823904245 1102 88-89 0.7141296245854463 1102 90-91 0.7525681117827823 1102 92-93 1.0246120537406291 1102 94-95 1.210705474820852 1102 96-97 0.9220503249914849 1102 98-99 1.3514108360469628 1102 100-101 1.8460645994796323 1103 1 0.13805609325263646 1103 2 0.1700001996267133 1103 3 0.12143933293126707 1103 4 0.1691130888218879 1103 5 0.19988096672398115 1103 6 0.07375424229649497 1103 7 0.23756141098288452 1103 8 0.13124673831228506 1103 9 0.10271920545412883 1103 10-11 0.17417264532991794 1103 12-13 0.04790324452726935 1103 14-15 0.20307771867824442 1103 16-17 0.14474772050187568 1103 18-19 0.20330062382644343 1103 20-21 0.20396581483512222 1103 22-23 0.36380483431946686 1103 24-25 0.21522093506637674 1103 26-27 0.14758985647006995 1103 28-29 0.1467882289103244 1103 30-31 0.23031141818882617 1103 32-33 0.30563762989527987 1103 34-35 0.41061304897716155 1103 36-37 0.4095862543639086 1103 38-39 0.3614352588687382 1103 40-41 0.4841411764012449 1103 42-43 0.6096783831795136 1103 44-45 0.6432051569963164 1103 46-47 0.6927648254334784 1103 48-49 0.43040448400623177 1103 50-51 0.45283339521596844 1103 52-53 0.6440484658470638 1103 54-55 0.776136329874916 1103 56-57 0.6291657060236489 1103 58-59 0.6642742160761514 1103 60-61 0.5834324409109826 1103 62-63 0.6602531667620823 1103 64-65 0.5699590926751696 1103 66-67 0.38499349015009443 1103 68-69 0.21621437556741796 1103 70-71 0.3497242861821235 1103 72-73 -0.01712915585449437 1103 74-75 -0.004358070163220873 1103 76-77 -0.26888682709717315 1103 78-79 0.1397795647720308 1103 80-81 0.25351788764510985 1103 82-83 0.27061021985275957 1103 84-85 0.4738276247951525 1103 86-87 0.6873016016138749 1103 88-89 0.7455409287280936 1103 90-91 0.5783290669013716 1103 92-93 0.6483636016575929 1103 94-95 0.9681135169276409 1103 96-97 0.8661728907359745 1103 98-99 1.4211923967606133 1103 100-101 1.2202953773219036 1104 1 -0.024302738515864064 1104 2 0.001538433428834196 1104 3 0.03805333834340274 1104 4 0.05096027826908767 1104 5 0.026952371922469354 1104 6 0.04134203365751432 1104 7 0.13209924138536877 1104 8 0.10133110597036676 1104 9 -0.14788372984092035 1104 10-11 0.04627331864373474 1104 12-13 0.05253541504239223 1104 14-15 -0.014827338706652426 1104 16-17 -0.19699410026749575 1104 18-19 -0.008496305142323024 1104 20-21 0.16675609376951428 1104 22-23 0.21229191254609958 1104 24-25 0.07062207531959785 1104 26-27 -0.06335525867196878 1104 28-29 -0.09706425494070459 1104 30-31 -0.013634557423671367 1104 32-33 0.0823957511263913 1104 34-35 0.03288469102144376 1104 36-37 0.2175056990920936 1104 38-39 0.09816245856541173 1104 40-41 0.1780448210217429 1104 42-43 -0.1847315020160636 1104 44-45 -0.15549149859560885 1104 46-47 -0.20888530415648887 1104 48-49 -0.3504205288488791 1104 50-51 -0.22199013908424803 1104 52-53 -0.3727584096063197 1104 54-55 -0.1532185250310576 1104 56-57 -0.35346387096075205 1104 58-59 -0.29002302886014064 1104 60-61 -0.12649811548618572 1104 62-63 -0.23552342947989757 1104 64-65 -0.17116048848173904 1104 66-67 0.07499645072253713 1104 68-69 0.06170019511792901 1104 70-71 0.12122782415276134 1104 72-73 -0.021963026188345225 1104 74-75 0.006478317522876864 1104 76-77 0.022299323656557846 1104 78-79 0.4321343625441578 1104 80-81 0.6989507285307859 1104 82-83 0.7175363319286063 1104 84-85 0.6647854132648483 1104 86-87 0.5450357075815901 1104 88-89 0.4656516991521471 1104 90-91 0.5874834685454466 1104 92-93 0.6890576221669171 1104 94-95 1.0727541709544504 1104 96-97 0.8378829762463091 1104 98-99 0.8721576991978068 1104 100-101 1.1222337731764114 1105 1 -0.0214123171166527 1105 2 0.07205428447959861 1105 3 0.1129418350382565 1105 4 0.03104867334494088 1105 5 0.10129744247533523 1105 6 0.0686041921865268 1105 7 0.04152204879547838 1105 8 0.11409573178865884 1105 9 0.18902197142276123 1105 10-11 0.2077802410179217 1105 12-13 0.276135083266432 1105 14-15 0.3610250243558468 1105 16-17 0.1733146431731143 1105 18-19 0.046855769382766255 1105 20-21 0.26737994025079104 1105 22-23 0.21205132450180741 1105 24-25 -0.1682271735577956 1105 26-27 -0.19642484245464686 1105 28-29 -0.0030422789894188895 1105 30-31 0.1289256358543298 1105 32-33 0.19217203789387582 1105 34-35 0.4350999517061567 1105 36-37 0.1898035841044745 1105 38-39 0.30360530039812517 1105 40-41 0.486466075545394 1105 42-43 0.3709183633051012 1105 44-45 0.40402628220680015 1105 46-47 0.45590152305923226 1105 48-49 0.5157855995847598 1105 50-51 0.6332810103713982 1105 52-53 0.5287039619188221 1105 54-55 0.5681451885715845 1105 56-57 0.5007061255229601 1105 58-59 0.5198939093793911 1105 60-61 0.8352736219801002 1105 62-63 0.7651941285682824 1105 64-65 0.6907478626152894 1105 66-67 0.7308192702461724 1105 68-69 0.8446536068414687 1105 70-71 0.8365574886586273 1105 72-73 0.8138204916799445 1105 74-75 0.7609178399135494 1105 76-77 0.6801612530380794 1105 78-79 0.8715869322095422 1105 80-81 0.7599440038425875 1105 82-83 1.027585341227418 1105 84-85 0.8633050396972344 1105 86-87 0.863793835840255 1105 88-89 1.1194774696648047 1105 90-91 1.1291775448640102 1105 92-93 1.3109844463879092 1105 94-95 1.1789603330367662 1105 96-97 1.2727038054506714 1105 98-99 1.1684973960568712 1105 100-101 0.5114673740728115 1106 1 0.01280830115705811 1106 2 -0.0187588043861453 1106 3 0.07789771299822945 1106 4 -0.030828753770933304 1106 5 0.07705252276694807 1106 6 0.049485987101796525 1106 7 0.11937060483813866 1106 8 0.1074786368193017 1106 9 0.08343587087456683 1106 10-11 0.06530197909527402 1106 12-13 -0.07844787666445541 1106 14-15 0.091560059596965 1106 16-17 -0.10084187098388497 1106 18-19 -0.19435978676484922 1106 20-21 0.0897396822196086 1106 22-23 0.03823551484421017 1106 24-25 0.0270659372680484 1106 26-27 -0.013446342361795871 1106 28-29 0.04442786899905826 1106 30-31 0.11857774588089143 1106 32-33 0.2868378326153831 1106 34-35 0.1355908245394204 1106 36-37 0.18460017060062484 1106 38-39 0.03662780142749966 1106 40-41 0.10511439906934328 1106 42-43 0.2142653409655466 1106 44-45 0.02592825296434853 1106 46-47 0.15870950943104845 1106 48-49 0.21819475177955638 1106 50-51 0.07425193745901737 1106 52-53 0.07941564679137514 1106 54-55 0.23657350741286365 1106 56-57 0.1186076441607895 1106 58-59 0.03305947400425424 1106 60-61 0.13191744045403908 1106 62-63 0.438838778619548 1106 64-65 0.172680028510932 1106 66-67 0.571564944833014 1106 68-69 0.3728214724327401 1106 70-71 0.567748097486259 1106 72-73 0.8669058050554206 1106 74-75 0.7170654570004409 1106 76-77 0.2939331424948115 1106 78-79 0.4090741154229782 1106 80-81 0.3738281158713903 1106 82-83 0.3339594218500501 1106 84-85 0.47458865600906464 1106 86-87 0.608715158936306 1106 88-89 0.5186838380939314 1106 90-91 0.5679355074188095 1106 92-93 0.5720286814578657 1106 94-95 0.8142248634377793 1106 96-97 0.5843620352405132 1106 98-99 0.6095127277593413 1106 100-101 1.0422664149331275 1107 1 -0.06001825755761914 1107 2 0.07350226897859358 1107 3 0.025466820421506497 1107 4 0.008505857941223383 1107 5 0.007120466234198375 1107 6 0.04087665809285568 1107 7 0.007802854705957429 1107 8 0.027310080203690745 1107 9 -0.07344172829843387 1107 10-11 -0.031700932767606105 1107 12-13 0.050909067221027726 1107 14-15 0.09486727296786057 1107 16-17 0.020540198569392487 1107 18-19 -0.11498508761789594 1107 20-21 -0.03226184867848758 1107 22-23 0.06075707762287408 1107 24-25 0.020499647012925948 1107 26-27 0.044903759829587386 1107 28-29 -0.08771152164036522 1107 30-31 -0.12335465786586042 1107 32-33 -0.059343997044585706 1107 34-35 0.009678401380249113 1107 36-37 -0.1053235212311634 1107 38-39 0.010518097585254793 1107 40-41 -0.23335375588280272 1107 42-43 -0.0652253592230565 1107 44-45 -0.16361665995596297 1107 46-47 -0.2732247092979847 1107 48-49 -0.0862787849896165 1107 50-51 -0.3622260499623664 1107 52-53 -0.3312643402670119 1107 54-55 -0.03103852828704845 1107 56-57 0.01710910009223454 1107 58-59 -0.24520966419481027 1107 60-61 0.03971002743509189 1107 62-63 -0.04951298072958821 1107 64-65 -0.3357225503421617 1107 66-67 -0.3291657828170571 1107 68-69 -0.13777407849330103 1107 70-71 0.029416460766800157 1107 72-73 0.05532385071406054 1107 74-75 0.178771698172131 1107 76-77 0.10041499661758024 1107 78-79 0.26622632930680723 1107 80-81 0.23796169856750637 1107 82-83 -0.029643870369987724 1107 84-85 -0.04247691219875804 1107 86-87 0.0641407722819487 1107 88-89 0.35611824226680966 1107 90-91 0.4792894052905474 1107 92-93 0.35329490779404793 1107 94-95 0.5431099119293705 1107 96-97 0.5455103666404959 1107 98-99 0.6851951216420638 1107 100-101 0.6002953441724941 1108 1 0.035126452844174594 1108 2 -0.02264394771447087 1108 3 0.08521529880929535 1108 4 0.04247159707222181 1108 5 -0.022234473401262278 1108 6 -0.07670437360377491 1108 7 0.001524490789861943 1108 8 0.013779262516543156 1108 9 0.0728539886091184 1108 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-0.987876089889145 2316 80-81 -0.8696623202219591 2316 82-83 -1.0144318666784606 2316 84-85 -1.468085729353998 2316 86-87 -1.5761820550561083 2316 88-89 -1.7967639502526573 2316 90-91 -1.8128047857357217 2316 92-93 -1.9296404541520928 2316 94-95 -1.874876701092603 2316 96-97 -1.2771601332713622 2316 98-99 -1.1504747975798146 2316 100-101 -1.13692429144454 >>END_MODULE >>Per sequence quality scores pass #Quality Count 3 3.0 4 11.0 5 59.0 6 171.0 7 234.0 8 317.0 9 564.0 10 829.0 11 1288.0 12 1798.0 13 2496.0 14 3057.0 15 4066.0 16 5215.0 17 7355.0 18 7206.0 19 5653.0 20 5533.0 21 6235.0 22 6930.0 23 7752.0 24 9017.0 25 10519.0 26 12305.0 27 14514.0 28 17688.0 29 21142.0 30 25501.0 31 30739.0 32 37780.0 33 47832.0 34 66971.0 35 92469.0 36 122456.0 37 155389.0 38 81513.0 39 2826.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.889100194606414 21.65239429893071 16.3864506670901 18.07205483937278 2 13.724119578187294 26.0342664572074 37.24720485925882 22.994409105346485 3 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22.273503770389475 22-23 18.219706585335643 29.160764894234102 29.34189565543705 23.2776328649932 24-25 21.934556201770224 29.666955879241996 29.22767978955953 19.17080812942825 26-27 21.836741951821914 26.290388051501473 33.59644507887221 18.276424917804405 28-29 17.784109791975553 31.068892232715623 32.287999136655 18.85899883865382 30-31 18.729742357741223 30.510967792571552 30.33308683852628 20.426203011160943 32-33 19.000396108570538 30.442599208028128 30.564620269231195 19.99238441417014 34-35 21.476760722742167 27.76306969790074 30.024536634157617 20.735632945199473 36-37 21.062586823923382 27.484045864497947 32.97196181434017 18.4814054972385 38-39 21.33258108630195 28.006279627392423 32.67429952105576 17.98683976524987 40-41 18.887492817796776 29.647337725131795 32.79303542443525 18.672134032636176 42-43 17.728116714128383 31.26384300105225 29.587782102885228 21.42025818193413 44-45 19.511904980899295 29.45874186763795 29.103033424697855 21.9263197267649 46-47 21.200082411619057 30.161671484230805 28.231130253280828 20.40711585086931 48-49 20.819199270056878 28.78222627746178 29.95685584849756 20.441718603983784 50-51 19.031807874892543 30.202274391208533 32.349309012333414 18.41660872156551 52-53 19.231990856391633 31.544038566013395 29.919748158340415 19.304222419254554 54-55 17.15021344488143 28.775816038840713 31.769440285100064 22.30453023117779 56-57 20.773257888753584 29.9484445687138 28.325073917783556 20.95322362474906 58-59 22.011452916299525 28.57563321235355 28.925450577643208 20.487463293703716 60-61 19.547339233747394 28.927930656849266 31.925148051992647 19.599582057410693 62-63 20.896968388857584 28.563649976178052 30.912610548248182 19.626771086716182 64-65 19.82561241070607 31.11475610877763 30.34981757978968 18.709813900726616 66-67 19.110777536181228 28.926097210111735 29.53119232189 22.431932931817038 68-69 17.641251546773574 30.5280062594508 30.622253672092587 21.208488521683037 70-71 20.95371416167852 30.104864532095217 27.77021533344861 21.171205972777653 72-73 19.447888422470026 27.309478522453716 30.902109676699375 22.340523378376886 74-75 18.423218093945177 31.98840370698758 28.911572134068646 20.676806064998594 76-77 20.48171919475345 27.611828546950008 29.553937796042014 22.35251446225453 78-79 19.19629142483973 27.84767125639634 31.738118670725985 21.217918648037944 80-81 20.180321928110367 29.75336340742712 29.552978457391525 20.51333620707099 82-83 18.871139627657943 28.805984060002476 30.97023299277807 21.35264331956151 84-85 18.70383962356127 28.395334249412734 30.083274928672733 22.817551198353264 86-87 19.3404696937895 29.291236907773527 29.86040277353287 21.507890624904107 88-89 20.189864765843307 27.549664470030134 29.27996216744109 22.980508596685464 90-91 19.32652235970139 26.351869940316273 31.18140787613457 23.140199823847762 92-93 19.72878213170181 29.681163259274516 30.365033056057335 20.22502155296634 94-95 18.90448606279789 29.073900681300746 29.60558628593293 22.41602696996844 96-97 16.41128087776678 28.62975866809413 30.506675594438782 24.452284859700306 98-99 18.97629848191084 28.864603223072894 28.181346597451906 23.97775169756436 100-101 19.321339661283005 26.867235691598296 30.110371215187083 23.70105343193162 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 14892.0 1 8547.0 2 1826.5 3 1259.0 4 881.0 5 870.5 6 1604.0 7 3059.5 8 4057.5 9 3866.0 10 2724.0 11 1628.0 12 1375.5 13 1274.5 14 1161.5 15 1063.0 16 1014.0 17 1039.5 18 1063.0 19 1175.0 20 1303.5 21 1485.0 22 1803.5 23 2136.5 24 2488.5 25 3193.5 26 4515.0 27 5857.0 28 6664.0 29 7703.5 30 9431.0 31 11156.0 32 13557.5 33 16861.0 34 20077.5 35 22242.5 36 24804.5 37 27776.0 38 27479.0 39 25699.5 40 24310.5 41 22448.5 42 20489.0 43 19555.5 44 19867.0 45 20227.0 46 24192.5 47 47772.5 48 111419.5 49 108179.0 50 45340.0 51 23895.0 52 14168.5 53 8205.5 54 5465.5 55 4140.0 56 3194.5 57 2456.0 58 1912.5 59 1485.5 60 1095.0 61 783.5 62 550.0 63 410.5 64 252.5 65 130.0 66 83.5 67 61.5 68 41.5 69 34.5 70 32.0 71 17.5 72 6.5 73 9.0 74 8.0 75 5.5 76 4.5 77 2.0 78 3.0 79 3.0 80 1.5 81 1.5 82 1.0 83 0.0 84 0.0 85 0.0 86 0.0 87 1.0 88 1.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.3708459186714298 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0010423909751015718 16-17 0.002759270228210043 18-19 2.452684647297816E-4 20-21 0.0 22-23 0.0 24-25 6.13171161824454E-5 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.014900059232334232 36-37 0.025201334750985058 38-39 0.012018154771759298 40-41 0.006070394502062095 42-43 0.004782735062230741 44-45 0.0022074161825680343 46-47 0.0017168792531084714 48-49 0.0033111242738520516 50-51 7.358053941893449E-4 52-53 0.0 54-55 0.0 56-57 0.0 58-59 9.197567427366811E-4 60-61 0.0012263423236489079 62-63 0.0010423909751015718 64-65 9.810738589191264E-4 66-67 0.0029432215767573795 68-69 0.0031884900414871605 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.08903245269691072 78-79 0.0031271729253047158 80-81 3.0658558091222697E-4 82-83 0.0 84-85 1.8395134854733622E-4 86-87 0.08976825809110006 88-89 0.42112595394103497 90-91 0.37618050777930256 92-93 0.0 94-95 9.197567427366811E-4 96-97 0.0 98-99 0.0 100-101 3.6790269709467243E-4 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 815433.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 27.820601138895228 #Duplication Level Percentage of deduplicated Percentage of total 1 75.41724252393858 20.98153023253823 2 12.698090437602788 7.065370185803334 3 4.249651196834568 3.5468355277968975 4 2.0119327026723446 2.2389270895738713 5 1.0807379919343818 1.5033390304628504 6 0.6991270278562352 1.1670080512445768 7 0.4827087174726534 0.9400472686552052 8 0.3455051248431006 0.7689728215763281 9 0.29439908043736046 0.7371323453254806 >10 2.214170984222661 12.639718142527848 >50 0.2933612814516841 5.692913463114063 >100 0.17822249278761265 8.837847440719255 >500 0.010146330034938346 2.063444876766228 >1k 0.016763501796854655 12.121465336269154 >5k 0.005734882193660804 10.586123909495496 >10k+ 0.0022057239206387707 9.109324278131194 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 22830 2.7997395248904566 No Hit GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 15318 1.8785111713653972 No Hit GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA 13172 1.6153381087103416 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 12887 1.5803873524863479 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 10016 1.2283044713667461 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 9347 1.1462621699146343 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 8979 1.1011327724043545 No Hit GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT 8720 1.0693705062218477 No Hit ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC 6819 0.8362428304961903 No Hit TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC 6413 0.7864533321560446 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 6360 0.7799537178407056 No Hit GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT 6301 0.772718298131177 No Hit GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA 6234 0.7645018045627293 No Hit CTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTG 5940 0.7284473402474514 No Hit GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG 5657 0.6937418524881872 No Hit TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT 5231 0.6414996695007438 No Hit GCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCT 5180 0.6352453236501344 No Hit CTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCA 5075 0.6223687292518207 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4882 0.598700322405397 No Hit ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC 4872 0.597473980081748 No Hit TGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCAC 4683 0.5742961101647837 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4414 0.541307501658628 No Hit GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA 4370 0.5359115954345728 No Hit GGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGT 4276 0.5243839775922731 No Hit GTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTG 4186 0.5133468966794329 No Hit TATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGG 3947 0.484037315144224 No Hit GATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATA 3723 0.4565672470944884 No Hit AAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGC 3650 0.44761494813185143 No Hit CCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCAC 3489 0.42787083672110404 No Hit TGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGAT 3350 0.4108246784223841 No Hit ACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCA 3188 0.3909579327792719 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3072 0.3767323618249446 No Hit ATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTG 2973 0.36459157282082033 No Hit GTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTG 2702 0.3313576958499349 No Hit GTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCA 2592 0.31786793028979693 No Hit TACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACC 2486 0.30486870165911856 No Hit CAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAG 2466 0.3024160170118207 No Hit ACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGT 2418 0.296529573858306 No Hit GCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAG 2282 0.2798513182566808 No Hit ATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATAC 2268 0.2781344390035723 No Hit GATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTT 2051 0.25152281058039105 No Hit CAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGT 1931 0.23680670269660414 No Hit ACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTG 1856 0.22760913526923732 No Hit CCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATA 1666 0.20430863111990807 No Hit GTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGC 1621 0.198790090663488 No Hit TGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGT 1586 0.1944978925307168 No Hit GCTTTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC 1389 0.17033894875483332 No Hit AAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTAC 1270 0.1557454751034113 No Hit AGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAG 1236 0.15157591120300504 No Hit AACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACG 1217 0.14924586078807212 No Hit CTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCT 1194 0.14642527344367962 No Hit ATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGT 1176 0.14421785726111158 No Hit AGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTA 1104 0.13538819253083945 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1081 0.13256760518644695 No Hit GCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAAC 1055 0.12937911514495978 No Hit GCTTTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGT 1044 0.128030138588946 No Hit ATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCA 997 0.12226632966779612 No Hit CACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACT 986 0.12091735311178234 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 962 0.11797413153502496 No Hit GTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTT 907 0.11122924875495595 No Hit TTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGA 859 0.1053428056014412 No Hit AGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAG 859 0.1053428056014412 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 6.13171161824454E-5 0.0 18-19 0.0 0.0 0.0 1.226342323648908E-4 0.0 20-21 0.0 0.0 0.0 6.13171161824454E-4 0.0 22-23 0.0 0.0 0.0 0.001962147717838253 0.0 24-25 0.0 0.0 0.0 0.0031884900414871605 0.0 26-27 0.0 0.0 0.0 0.006867517012433885 0.0 28-29 0.0 0.0 0.0 0.0199893798754772 0.0 30-31 0.0 0.0 0.0 0.0513224262447068 0.0 32-33 0.0 0.0 0.0 0.10319670653505561 0.0 34-35 0.0 0.0 0.0 0.17781963692909164 0.0 36-37 0.0 0.0 0.0 0.28162951462597174 0.0 38-39 0.0 0.0 0.0 0.41180575228130334 0.0 40-41 0.0 0.0 0.0 0.5452317970943045 0.0 42-43 0.0 0.0 0.0 0.6882233120317671 0.0 44-45 0.0 0.0 0.0 0.8587462121351478 0.0 46-47 0.0 0.0 0.0 1.06967709180276 0.0 48-49 0.0 0.0 0.0 1.2861878290429747 0.0 50-51 0.0 0.0 0.0 1.5008590527977161 0.0 52-53 0.0 0.0 0.0 1.6930268949135 0.0 54-55 0.0 0.0 0.0 1.9228434463653055 0.0 56-57 0.0 0.0 0.0 2.205760620431108 0.0 58-59 0.0 0.0 0.0 2.509832199579855 0.0 60-61 0.0 0.0 0.0 2.9090066259275744 0.0 62-63 0.0 0.0 0.0 3.2770932743707943 0.0 64-65 0.0 0.0 0.0 3.6161769268597173 0.0 66-67 0.0 0.0 0.0 4.023077309846425 0.0 68-69 1.226342323648908E-4 0.0 0.0 4.488167636090274 0.0 70-71 1.226342323648908E-4 0.0 0.0 5.010405514616161 0.0 72-73 1.226342323648908E-4 0.0 0.0 5.542883351544517 0.0 74-75 1.226342323648908E-4 0.0 0.0 6.1533565602569436 0.0 76-77 1.226342323648908E-4 0.0 0.0 6.684914640442562 0.0 78-79 1.226342323648908E-4 0.0 0.0 7.396438456623659 0.0 80-81 1.226342323648908E-4 0.0 0.0 8.127951652680231 0.0 82-83 1.226342323648908E-4 0.0 0.0 8.777913084214154 0.0 84-85 1.226342323648908E-4 0.0 0.0 9.65388940599657 0.0 86-87 1.226342323648908E-4 0.0 0.0 10.58646142601538 0.0 88-89 1.226342323648908E-4 0.0 0.0 11.434661094167147 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GCGTCAG 35 3.366129E-6 68.025826 1 CGTCAGA 45 1.5241874E-5 52.72729 2 GTAAGCG 260 0.0 41.97904 94-95 CTGACCG 65 1.3353434E-4 36.50351 4 CTCCGAC 125 0.0 32.2691 94-95 ACCGTGC 80 4.5177568E-4 29.659103 7 GGACAAT 320 0.0 26.693193 6 CTTTGCG 840 0.0 26.551958 2 CGTGCAA 90 8.99221E-4 26.365261 9 TGACCGT 90 8.9954305E-4 26.363646 5 TAGGACA 470 0.0 26.25146 4 CGCAGAA 260 0.0 25.554026 9 GCTTTGC 875 0.0 25.033504 1 CCGTGCA 95 0.0012331089 24.976086 8 CATTCCG 115 1.289122E-4 24.76042 9 GACAATG 345 0.0 24.758904 7 TTTGCGT 905 0.0 24.64491 3 ACTCCCG 135 1.3520956E-5 24.60607 6 CTCTTAG 155 1.3796343E-6 24.577072 1 TTGCGTT 955 0.0 23.851507 4 >>END_MODULE