##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938876_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 818902 Sequences flagged as poor quality 0 Sequence length 101 %GC 40 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.14659141142652 33.0 31.0 34.0 31.0 34.0 2 32.210031236948986 34.0 31.0 34.0 30.0 34.0 3 32.25416838644917 34.0 31.0 34.0 30.0 34.0 4 35.73469841324115 37.0 35.0 37.0 35.0 37.0 5 35.78695863485496 37.0 35.0 37.0 35.0 37.0 6 35.91572129510003 37.0 36.0 37.0 35.0 37.0 7 35.88305926716506 37.0 36.0 37.0 35.0 37.0 8 35.88728687926028 37.0 36.0 37.0 35.0 37.0 9 37.50796310181194 39.0 37.0 39.0 35.0 39.0 10-11 37.43526392657485 39.0 37.0 39.0 35.0 39.0 12-13 37.37871808836711 39.0 37.0 39.0 35.0 39.0 14-15 38.51057879941678 40.0 38.0 41.0 34.0 41.0 16-17 38.469195947744666 40.0 38.0 41.0 34.5 41.0 18-19 38.46645508253735 40.0 38.0 41.0 34.0 41.0 20-21 38.32786096504832 40.0 38.0 41.0 34.0 41.0 22-23 38.46107776022039 40.0 38.0 41.0 34.0 41.0 24-25 38.37645896578589 40.0 38.0 41.0 34.0 41.0 26-27 38.19855123079441 40.0 38.0 41.0 34.0 41.0 28-29 38.10523420384857 40.0 38.0 41.0 34.0 41.0 30-31 38.04445464780889 40.0 38.0 41.0 34.0 41.0 32-33 37.866391827105076 40.0 37.0 41.0 34.0 41.0 34-35 37.571681348928195 40.0 37.0 41.0 33.0 41.0 36-37 37.25746975828609 40.0 36.0 41.0 32.5 41.0 38-39 37.014823507574775 39.0 35.5 41.0 31.5 41.0 40-41 36.96785634911137 39.0 36.0 41.0 32.0 41.0 42-43 36.8495601427277 39.0 36.0 41.0 31.0 41.0 44-45 36.84044488839935 40.0 36.0 41.0 31.0 41.0 46-47 36.61517922779527 40.0 36.0 41.0 31.0 41.0 48-49 36.435619280451135 40.0 36.0 41.0 30.0 41.0 50-51 35.25873608807892 38.0 34.5 40.0 28.5 40.5 52-53 35.43491712072995 38.5 35.0 39.5 28.5 40.5 54-55 36.0414518464969 39.0 35.0 40.5 29.0 41.0 56-57 36.019397925514895 39.0 35.5 41.0 28.5 41.0 58-59 35.91418203887645 39.0 35.0 41.0 28.0 41.0 60-61 35.56855948575043 39.0 35.0 40.5 28.0 41.0 62-63 35.24471609545465 38.5 35.0 40.0 28.0 41.0 64-65 34.77723769144538 38.0 34.5 40.0 26.0 41.0 66-67 34.30654156419205 37.0 34.0 39.5 26.0 41.0 68-69 33.927359439835286 37.0 34.0 39.0 26.0 41.0 70-71 33.339210919011066 36.0 33.5 38.5 25.5 40.0 72-73 32.815574391074875 35.5 33.0 37.5 24.0 40.0 74-75 32.36648768717136 35.0 33.0 37.0 22.5 39.0 76-77 31.890671899690073 35.0 32.0 36.5 21.0 39.0 78-79 31.41240038490564 35.0 31.5 36.0 19.5 38.5 80-81 30.97836371140869 35.0 31.0 36.0 18.0 37.5 82-83 30.584535145841627 34.5 31.0 35.0 13.0 37.0 84-85 30.21745459163612 34.0 31.0 35.0 7.0 36.5 86-87 29.900389179657637 34.0 31.0 35.0 2.0 36.0 88-89 29.361313685886714 34.0 29.5 35.0 2.0 36.0 90-91 28.85965719951838 34.0 29.0 35.0 2.0 35.5 92-93 28.49992489943852 34.0 29.0 35.0 2.0 35.0 94-95 28.02584436232907 34.0 29.0 35.0 2.0 35.0 96-97 27.370283013107795 34.0 27.0 35.0 2.0 35.0 98-99 26.756404917804574 34.0 25.5 35.0 2.0 35.0 100-101 24.143406659160682 31.0 13.0 34.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.10978439449618094 1101 2 0.05650406732600999 1101 3 0.09611190645914292 1101 4 0.136857291961185 1101 5 -0.03469617321432139 1101 6 0.057809758025669566 1101 7 0.027139886050150608 1101 8 0.1537108523751911 1101 9 0.11515383981769389 1101 10-11 0.20352253627073935 1101 12-13 0.2383928562105453 1101 14-15 0.00900374519167002 1101 16-17 0.10244518128683211 1101 18-19 0.13097397129767785 1101 20-21 0.25441929811915287 1101 22-23 0.15656705887042932 1101 24-25 0.2603348684920377 1101 26-27 0.2437951888104095 1101 28-29 0.21092437527501318 1101 30-31 0.3394227676583057 1101 32-33 0.255919442482913 1101 34-35 0.0941318884417015 1101 36-37 0.24602327476138441 1101 38-39 0.2803473114567865 1101 40-41 0.08325300143352621 1101 42-43 0.06021261597873462 1101 44-45 0.04424200356010033 1101 46-47 0.21858268447832074 1101 48-49 0.10185744182308554 1101 50-51 0.1738761458186957 1101 52-53 0.0028559923463191694 1101 54-55 -0.0010842436836355773 1101 56-57 0.10780804628794272 1101 58-59 -0.017022437138258795 1101 60-61 -0.02247489703230343 1101 62-63 -0.23851520749863653 1101 64-65 -0.1403927088308663 1101 66-67 -0.16855760835808553 1101 68-69 -0.2684602179118798 1101 70-71 -0.27741529682769794 1101 72-73 -0.3815100426875375 1101 74-75 -0.42761874475885264 1101 76-77 -0.16381282980584544 1101 78-79 -0.23133458638433524 1101 80-81 -0.2458385662973228 1101 82-83 -0.41736184180355096 1101 84-85 -0.4916845258966127 1101 86-87 -0.5066090293819947 1101 88-89 -0.40212283425529094 1101 90-91 -0.4169950946188763 1101 92-93 -0.33588709496833147 1101 94-95 -0.23587139748147834 1101 96-97 -0.32188113465387147 1101 98-99 -0.47520885497813126 1101 100-101 0.19023794426644258 1102 1 0.24478030083317037 1102 2 0.11286234471261736 1102 3 0.16726468196266353 1102 4 0.162820940233658 1102 5 0.1582217897789704 1102 6 0.20753466387142083 1102 7 0.288420383839572 1102 8 0.18422337898400087 1102 9 0.32121737346760426 1102 10-11 0.08648880448753715 1102 12-13 0.2143605981460297 1102 14-15 0.294351706547495 1102 16-17 0.348065780599093 1102 18-19 0.4486422208473755 1102 20-21 0.4715504590819819 1102 22-23 0.3998157898348964 1102 24-25 0.2625691897627149 1102 26-27 0.33860442411415903 1102 28-29 0.42017196001383894 1102 30-31 0.28606760145559207 1102 32-33 0.3127329351961876 1102 34-35 0.38248705464441457 1102 36-37 0.34520617962466105 1102 38-39 0.3997275308771222 1102 40-41 0.44481350577281376 1102 42-43 0.22284484129394144 1102 44-45 0.6528804512430852 1102 46-47 0.7371237029816768 1102 48-49 0.648041329165487 1102 50-51 0.6638409403168097 1102 52-53 0.5258795718452589 1102 54-55 0.47948886913771815 1102 56-57 0.5758209822630533 1102 58-59 0.616592267299275 1102 60-61 0.6590700513875447 1102 62-63 0.6081468196832844 1102 64-65 0.580094437067288 1102 66-67 0.5936693443191743 1102 68-69 0.5990925903409448 1102 70-71 0.5017389523945113 1102 72-73 0.5430142736707353 1102 74-75 0.45716510983423575 1102 76-77 0.3621418124080087 1102 78-79 0.33397489453919604 1102 80-81 0.28245192002984254 1102 82-83 0.03414953857961578 1102 84-85 0.1882557066234476 1102 86-87 0.3693063136669643 1102 88-89 0.3623114215167753 1102 90-91 0.4225889892193422 1102 92-93 0.23352751122128623 1102 94-95 0.24146199043402916 1102 96-97 0.1860081726983438 1102 98-99 -0.015865478524883514 1102 100-101 0.6922282832217412 1103 1 0.018976904979012943 1103 2 0.02233127702419324 1103 3 0.21740056839296074 1103 4 0.11655422391618231 1103 5 0.1486558423957618 1103 6 0.04879742772301654 1103 7 0.22294011023747373 1103 8 0.038978424094381126 1103 9 0.06454064597869547 1103 10-11 0.17541193719240056 1103 12-13 0.26889727768271143 1103 14-15 0.34092220743271895 1103 16-17 0.15814244537119038 1103 18-19 0.15509486638632097 1103 20-21 0.24062845658637144 1103 22-23 0.2656018079051776 1103 24-25 0.4162621321612363 1103 26-27 0.12607608722034058 1103 28-29 0.2762915858035129 1103 30-31 0.33253357431750885 1103 32-33 0.3893258442764065 1103 34-35 0.3599914807529032 1103 36-37 0.2749127171992214 1103 38-39 0.3721345825988962 1103 40-41 0.4723550066645714 1103 42-43 0.3389779250666223 1103 44-45 0.386803551282938 1103 46-47 0.2723120820786846 1103 48-49 -0.027705392901033576 1103 50-51 0.015593254632584319 1103 52-53 0.2371184575765355 1103 54-55 0.418219114974562 1103 56-57 0.434913123715603 1103 58-59 0.5005081810375245 1103 60-61 0.25780575228061053 1103 62-63 -0.061073433927830933 1103 64-65 -0.03208781407435879 1103 66-67 0.22994448820853108 1103 68-69 0.2129538228570027 1103 70-71 -0.02224777907952813 1103 72-73 0.10240742316541684 1103 74-75 0.3510585500474761 1103 76-77 0.08773696884072635 1103 78-79 -0.14377615237428998 1103 80-81 -0.13524696086700772 1103 82-83 -0.10901792184672843 1103 84-85 -0.3952499346233118 1103 86-87 -0.10234780963690326 1103 88-89 0.025136683326810072 1103 90-91 -0.2271441376711465 1103 92-93 -0.4934559080210157 1103 94-95 -0.6112523083462627 1103 96-97 -0.3362922277746456 1103 98-99 -0.3821561709577068 1103 100-101 -0.3615750669014055 1104 1 0.08718797319008331 1104 2 0.17033642502934043 1104 3 0.1852255362179278 1104 4 0.07150917887113195 1104 5 0.20270989644981796 1104 6 0.16720154612713856 1104 7 0.16631205360941692 1104 8 0.16767855279450572 1104 9 0.1533437347817781 1104 10-11 0.12135788313834439 1104 12-13 0.27340178218721434 1104 14-15 0.32226068877120184 1104 16-17 0.14141142864017553 1104 18-19 0.3500755613670137 1104 20-21 0.37383308979101315 1104 22-23 0.17808572038908466 1104 24-25 0.3216675375666398 1104 26-27 0.41886888001313594 1104 28-29 0.18620149571342637 1104 30-31 0.2257124674963933 1104 32-33 0.21493716988774025 1104 34-35 0.13283575359717048 1104 36-37 0.06384450613100512 1104 38-39 0.32580253626684197 1104 40-41 0.39835243266199427 1104 42-43 0.3486304347191336 1104 44-45 0.2703299348093182 1104 46-47 0.36626317602978276 1104 48-49 0.48580812061247514 1104 50-51 0.44159068063000717 1104 52-53 0.3516615721196459 1104 54-55 0.41114060789605134 1104 56-57 0.5256467144491879 1104 58-59 0.4664026469319893 1104 60-61 0.46629996077481195 1104 62-63 0.5116421387877423 1104 64-65 0.5766637946772519 1104 66-67 0.639210897474932 1104 68-69 0.42981353971671865 1104 70-71 0.5459632891315351 1104 72-73 0.5155348362928223 1104 74-75 0.4417921407810681 1104 76-77 0.2955876766914365 1104 78-79 0.5717965631984328 1104 80-81 0.4709306453105988 1104 82-83 0.4591931463643384 1104 84-85 0.5539135145401346 1104 86-87 0.6936624863733947 1104 88-89 0.8147119729020993 1104 90-91 0.5257516152246069 1104 92-93 0.36046944590433583 1104 94-95 0.3662251691312157 1104 96-97 0.25830236681994734 1104 98-99 0.3340600452585072 1104 100-101 0.4736887683624289 1105 1 0.05910019478383077 1105 2 0.12177630895330083 1105 3 0.24159783208067154 1105 4 0.1624911526556545 1105 5 0.28291220977314424 1105 6 0.17499077302464627 1105 7 0.15667347963963607 1105 8 0.21264654171154262 1105 9 0.2251764410093955 1105 10-11 0.06121721471741637 1105 12-13 0.2479593242606768 1105 14-15 0.2555046697858785 1105 16-17 0.27936730990816017 1105 18-19 0.27264235017584326 1105 20-21 0.30463914607476994 1105 22-23 0.37134412893411906 1105 24-25 0.36196215429438183 1105 26-27 0.2796694439984151 1105 28-29 0.4325187866167042 1105 30-31 0.36583739679329597 1105 32-33 0.40214093416156516 1105 34-35 0.2933223548481081 1105 36-37 0.405558923004655 1105 38-39 0.4276002393384388 1105 40-41 0.31849031394637706 1105 42-43 0.18059889089141024 1105 44-45 0.22812940853236086 1105 46-47 0.38003327005465337 1105 48-49 0.5058681260228042 1105 50-51 0.300081200267023 1105 52-53 0.39853711007798864 1105 54-55 0.20336613642731294 1105 56-57 0.21010514922609502 1105 58-59 0.31623418912021606 1105 60-61 0.42897774319781945 1105 62-63 0.2944291113836357 1105 64-65 0.39752387222522856 1105 66-67 0.4724368440766753 1105 68-69 0.5962449392691411 1105 70-71 0.3395602527921895 1105 72-73 0.5802980526484021 1105 74-75 0.5305485040597233 1105 76-77 0.4237934985278251 1105 78-79 0.3631425042258982 1105 80-81 0.26836320070493613 1105 82-83 0.2838052015496437 1105 84-85 0.4893962219973673 1105 86-87 0.6202124212035791 1105 88-89 0.7818303196382779 1105 90-91 0.5506112337148004 1105 92-93 0.753087632790038 1105 94-95 0.8834218016144746 1105 96-97 0.7774983599012941 1105 98-99 0.7802174856834654 1105 100-101 1.0917748253019894 1106 1 -0.08664680191051843 1106 2 -0.027219901640911814 1106 3 0.10156719433174288 1106 4 -0.043289706813830264 1106 5 0.027941638137249925 1106 6 0.11854475190072122 1106 7 0.144894071431942 1106 8 0.014339680468296478 1106 9 -0.007565637519995505 1106 10-11 -0.038964193006520986 1106 12-13 -0.018234338562834296 1106 14-15 -0.02864835770747476 1106 16-17 0.06361708818760548 1106 18-19 0.1886505739926605 1106 20-21 0.20237356516692984 1106 22-23 0.17732655254130947 1106 24-25 0.08364642422907309 1106 26-27 0.06688624638492513 1106 28-29 0.035199589648229335 1106 30-31 -0.00791492119428483 1106 32-33 0.17164831140900816 1106 34-35 0.18121641273732791 1106 36-37 -0.03928909763551047 1106 38-39 0.031474302065198856 1106 40-41 -0.05188378909321756 1106 42-43 -0.02933822109762474 1106 44-45 0.010415952110527371 1106 46-47 -0.0721033098050583 1106 48-49 -0.072544354828608 1106 50-51 -0.04750809264598388 1106 52-53 -0.2108598100219936 1106 54-55 -0.27767877206256486 1106 56-57 -0.22326177813018688 1106 58-59 -0.1652380353416092 1106 60-61 -0.05306289213233839 1106 62-63 -0.18339883346841646 1106 64-65 -0.44748539972510315 1106 66-67 -0.06758169982398954 1106 68-69 0.21469208915222993 1106 70-71 0.15769699390320824 1106 72-73 -0.12387835628492638 1106 74-75 -0.1330086998425628 1106 76-77 -0.105703397384449 1106 78-79 0.05157272272142066 1106 80-81 0.12596216869654953 1106 82-83 0.13437764370073424 1106 84-85 0.07821747492004505 1106 86-87 0.08558370234523949 1106 88-89 0.23187063246582085 1106 90-91 0.15339281881517763 1106 92-93 0.31065435051962 1106 94-95 0.46851814927229896 1106 96-97 0.559156023369809 1106 98-99 0.41310065910925076 1106 100-101 0.7545539916327151 1107 1 0.02659152903198958 1107 2 -0.016636172902167345 1107 3 -0.0017983494360933605 1107 4 -0.012403946137879984 1107 5 0.005781069383999693 1107 6 0.049047455370306636 1107 7 -0.07632855889421108 1107 8 0.052765205004448035 1107 9 -0.04495864160278984 1107 10-11 -0.03344769632477096 1107 12-13 -0.0818722785662942 1107 14-15 -0.0014457787708863634 1107 16-17 -0.1517580083648724 1107 18-19 0.15698529978359943 1107 20-21 0.03646565653316003 1107 22-23 -0.15404315283567627 1107 24-25 0.09430345403817597 1107 26-27 0.0918046693872796 1107 28-29 -0.04677859343104984 1107 30-31 -0.05278226990245116 1107 32-33 0.10609981447503714 1107 34-35 0.009475157633836773 1107 36-37 -0.1540273418778284 1107 38-39 -0.054693864777497936 1107 40-41 0.01806198387839686 1107 42-43 -0.0021671695031315608 1107 44-45 0.09827004357135394 1107 46-47 0.07595302955525085 1107 48-49 0.0876799622103519 1107 50-51 0.06640255886654245 1107 52-53 0.19066059605017216 1107 54-55 0.07759811681930984 1107 56-57 0.28433237039511994 1107 58-59 0.039886734114709554 1107 60-61 -0.0786481184198422 1107 62-63 -0.22143972443110727 1107 64-65 -0.09102702369849425 1107 66-67 -0.214129455865411 1107 68-69 -0.09371310024828006 1107 70-71 0.0808814130907578 1107 72-73 -0.0041075600619038255 1107 74-75 -0.0777412691633046 1107 76-77 -0.009567861340816108 1107 78-79 -0.03524411918472481 1107 80-81 -0.2433286237431922 1107 82-83 -0.3306057256263166 1107 84-85 -0.3565469370162049 1107 86-87 -0.2749109616521146 1107 88-89 -0.17020623119418943 1107 90-91 -0.015751371483858634 1107 92-93 -0.283625013258618 1107 94-95 -0.00597544279542106 1107 96-97 0.12299573836263633 1107 98-99 0.22494942244925653 1107 100-101 0.4565867170138027 1108 1 0.03057596395961326 1108 2 0.07089872584101187 1108 3 -0.13361699708844554 1108 4 -0.0775679352932599 1108 5 0.009282589556171672 1108 6 0.03906307634277795 1108 7 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2316 100-101 -3.8934925614656457 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 3057.0 3 546.0 4 428.0 5 477.0 6 459.0 7 568.0 8 688.0 9 797.0 10 965.0 11 1426.0 12 2101.0 13 3834.0 14 3722.0 15 3272.0 16 4519.0 17 6334.0 18 5459.0 19 3569.0 20 3506.0 21 3692.0 22 4058.0 23 4755.0 24 5559.0 25 6437.0 26 7639.0 27 9144.0 28 11202.0 29 13539.0 30 16897.0 31 21156.0 32 27142.0 33 36085.0 34 50954.0 35 84338.0 36 110302.0 37 169541.0 38 163158.0 39 27571.0 40 6.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 45.3116831120683 20.79038326621716 15.751099182974325 18.146834438740218 2 13.354004245807053 25.193743973190553 37.917232427993 23.5350193530094 3 23.318271650507032 27.419514514337116 32.72267722328894 16.53953661186691 4 10.88690158956782 18.06110374973719 42.86259112756147 28.189403533133518 5 10.536937773893271 41.30527525318893 36.18123254264637 11.976554430271433 6 24.48635159312887 39.57304732946824 18.22688032224319 17.713720755159702 7 22.278214594330986 37.16331751341719 23.31565560576264 17.24281228648919 8 21.19646587052199 30.779779343015644 22.228985092759288 25.794769693703074 9 21.406370124290635 23.088064358813586 26.532488025002355 28.973077491893424 10-11 22.438189427961557 25.800669880610677 29.138523268298734 22.62261742312903 12-13 28.998347568040593 25.189443013097236 27.280649435093157 18.53155998376901 14-15 23.69634849846996 27.7456544949949 31.01004686991018 17.547950136624962 16-17 17.383681635576327 30.730304189526613 32.18843153382946 19.6975826410676 18-19 16.792677806495536 34.061694222881734 31.299675901507797 17.845952069114933 20-21 19.82354246208137 29.70472454178918 28.152898085259697 22.318834910869754 22-23 18.049089984450028 29.068175372367993 29.518293219626212 23.36444142355576 24-25 21.836628899998413 29.319784281290158 29.366977333885956 19.476609484825477 26-27 21.865835922785323 26.035836833652954 33.66792945116252 18.430397792399205 28-29 17.830691776534564 30.711337584601537 32.60380804741598 18.85416259144791 30-31 18.665144265445484 30.37477720212321 30.53730657232343 20.422771960107873 32-33 19.024602528464168 30.254716727421194 30.686355046048757 20.03432569806588 34-35 21.495924456752544 27.74090318279061 29.954338962879802 20.808833397577043 36-37 21.11949178872342 27.258269915400458 33.18760614854103 18.434632147335094 38-39 21.47332180391767 27.860912763824402 32.60325979186888 18.062505640389055 40-41 18.808097557562878 29.61676707708415 33.04270874310132 18.532426622251652 42-43 17.670479099552132 31.165472519053978 29.5711135774338 21.592934803960084 44-45 19.307907913439372 29.454068773274145 29.231619373912082 22.0064039393744 46-47 21.033296416442177 29.996058651199224 28.41049454718691 20.56015038517169 48-49 20.702031462710874 28.683027833059366 30.017482012930675 20.597458691299092 50-51 19.090798012352494 29.98632617386451 32.52614574321574 18.39673007056726 52-53 19.09426107459406 31.333863436109823 30.19553424228562 19.376341247010497 54-55 17.10208512148371 28.463117748397877 32.228455934449016 22.206341195669392 56-57 20.739945544761078 29.734328160331152 28.545298754993166 20.980427539914608 58-59 21.86074544713571 28.346520968462606 29.299074942429403 20.493658641972278 60-61 19.643304084096464 28.329186419716734 32.35279683558592 19.674712660600886 62-63 21.046137223785948 28.245827821074375 31.1076801242522 19.600354830887476 64-65 19.91156991043383 30.65122005889198 30.82851010651527 18.608699924158916 66-67 19.179984436925636 28.55093283249796 29.897501125467002 22.371581605109405 68-69 17.60594435702502 30.283767134309404 31.108744440063234 21.00154406860234 70-71 20.99241141947482 29.762331750434214 28.028123687229616 21.217133142861357 72-73 19.45940667874836 27.108395630201937 31.23346878667419 22.198728904375507 74-75 18.5841273155469 31.613459879206214 29.417800963525437 20.38461184172145 76-77 20.65427417993154 27.393678910771023 29.88662563126468 22.06542127803276 78-79 19.41530339768577 27.585463920689975 32.189496946072865 20.809735735551396 80-81 20.53720994327616 29.6201448511191 29.820927972814786 20.02171723278995 82-83 19.164402862062932 28.572498534282442 31.338791020906807 20.924307582747815 84-85 19.214921826794086 28.192576538521752 30.424465312462583 22.168036322221575 86-87 19.98993191766273 29.117876371315788 30.359197254605842 20.53299445641564 88-89 20.912343953768808 27.33943478292904 29.578113372514252 22.1701078907879 90-91 19.819019862864806 26.23149638985401 31.573947506426293 22.375536240854892 92-93 20.553389055044125 29.478177027998008 30.72282645409714 19.245607462860722 94-95 19.645565857786632 28.923162333219928 29.944569384072878 21.486702424920566 96-97 17.431405060181774 28.153709162367967 30.902419552935395 23.51246622451486 98-99 20.04660000515867 28.757754896746718 28.49920716121956 22.69643793687505 100-101 20.527827187342858 26.863644540937216 30.187692218899144 22.420836052820786 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 16683.0 1 9242.0 2 1659.0 3 1306.5 4 908.5 5 913.5 6 1660.5 7 3127.0 8 4158.5 9 3973.5 10 2859.0 11 1818.5 12 1577.0 13 1525.5 14 1425.5 15 1399.0 16 1590.5 17 1622.5 18 1374.5 19 1328.5 20 1485.5 21 1689.5 22 1976.5 23 2313.5 24 2657.0 25 3363.0 26 4664.0 27 5859.0 28 6546.5 29 7551.0 30 9289.5 31 11103.5 32 13612.0 33 16816.5 34 20208.5 35 22204.5 36 24471.5 37 27651.0 38 27287.5 39 25499.5 40 24301.0 41 22413.5 42 20104.5 43 19061.5 44 19472.5 45 19789.0 46 24317.0 47 46749.0 48 114170.5 49 111572.0 50 43612.5 51 20672.0 52 11301.5 53 7886.0 54 6035.0 55 4595.5 56 3511.5 57 2679.5 58 2105.0 59 1678.0 60 1260.5 61 923.5 62 677.0 63 497.0 64 316.5 65 210.0 66 169.5 67 137.5 68 112.5 69 96.5 70 78.5 71 57.5 72 56.5 73 50.0 74 34.0 75 30.0 76 26.5 77 22.0 78 17.0 79 11.0 80 9.0 81 6.5 82 6.5 83 4.0 84 1.5 85 0.5 86 0.0 87 1.0 88 1.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.5 96 0.5 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.26364571096419354 2 0.08340436340367956 3 0.08682357595902807 4 0.09988985250982414 5 0.10221003245809632 6 0.07790920036829803 7 0.08975432957789821 8 0.08657934649078888 9 0.09024278851437657 10-11 0.08505291231429402 12-13 0.08718992016138684 14-15 0.09604323838505707 16-17 0.08486974021311464 18-19 0.09604323838505707 20-21 0.0978749593968509 22-23 0.10917057230291292 24-25 0.12028301310779556 26-27 0.1221757914866492 28-29 0.11600899741360993 30-31 0.10941480177115212 32-33 0.10453021240636852 34-35 0.3661610302575888 36-37 0.5611172032795133 38-39 0.5450591157427873 40-41 0.5083025807727909 42-43 0.5341298470390841 44-45 0.5567821302182678 46-47 0.5446317141733688 48-49 0.5661849647454763 50-51 0.5583696217618226 52-53 0.5610561459124536 54-55 0.551103795081707 56-57 0.5236279799047994 58-59 0.5189876200082549 60-61 0.46867634955098414 62-63 0.5409072147827212 64-65 0.5738781929950104 66-67 0.5856012074704909 68-69 0.5884709037223013 70-71 0.5841968880281158 72-73 0.576686831879761 74-75 0.5744887666656083 76-77 0.5573927038888659 78-79 0.5344961912414429 80-81 0.5301611181801974 82-83 0.543960083135711 84-85 0.5525081145240822 86-87 0.543044222629814 88-89 0.5568431875853277 90-91 0.5701536936043629 92-93 0.578457495524495 94-95 0.5777248071197775 96-97 0.5741834798303094 98-99 0.5787017249927342 100-101 0.5572705891547463 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 818902.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 28.551341614294795 #Duplication Level Percentage of deduplicated Percentage of total 1 76.19975260349969 21.756051675072687 2 12.245895223353802 6.992734757896704 3 4.170532320540319 3.572228789916127 4 1.9151510667492926 2.1872052939896056 5 1.0530552178252661 1.5033069631422393 6 0.7149362744621992 1.224743388277288 7 0.4423817846344972 0.8841415419928642 8 0.3656634853207333 0.8352146468212741 9 0.27255678488071466 0.7003675686980829 >10 2.1229670711582176 12.358696725981542 >50 0.29466204584337635 5.786843676101362 >100 0.16941995356800138 8.556887141026154 >500 0.009007136771969694 1.867636290344041 >1k 0.01544080589480519 10.582496732435294 >5k 0.006004757847979795 10.72638714232017 >10k+ 0.002573467649134198 10.465057665984574 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 21681 2.6475695504468177 No Hit GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 16133 1.9700770055513357 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 14048 1.7154677849119921 No Hit GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA 13053 1.5939636244630004 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 10403 1.2703595790460886 No Hit GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT 10139 1.238121289238517 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 9691 1.1834138883529408 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 9281 1.1333468473639092 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 7194 0.878493397256326 No Hit GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT 6404 0.7820227573018504 No Hit ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC 6265 0.7650488092592276 No Hit GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA 5892 0.7195000134326207 No Hit TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC 5704 0.696542443418138 No Hit CTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTG 5596 0.6833540521332223 No Hit GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG 5387 0.6578320727022281 No Hit GCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCT 5336 0.651604221262129 No Hit CTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCA 5317 0.6492840413138569 No Hit ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC 5261 0.6424456162031599 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5142 0.6279139628429288 No Hit TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT 5121 0.6253495534264173 No Hit GTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTG 4385 0.5354731091143995 No Hit TGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCAC 4373 0.5340077323049645 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4349 0.5310769786860944 No Hit GGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGT 4026 0.4916339195654669 No Hit GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA 3889 0.4749042009910832 No Hit CCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCAC 3774 0.4608610065673304 No Hit GATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATA 3668 0.4479168447506539 No Hit TATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGG 3645 0.4451082058659034 No Hit AAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGC 3307 0.40383342573348213 No Hit TGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGAT 3117 0.3806316262507602 No Hit ACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCA 3009 0.3674432349658445 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2901 0.35425484368092885 No Hit GTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTG 2776 0.3389905019159802 No Hit TACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACC 2701 0.329831896857011 No Hit ATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTG 2677 0.3269011432381408 No Hit GTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCA 2609 0.3185973413180087 No Hit CAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAG 2571 0.3139569814214644 No Hit ACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGT 2358 0.2879465430539918 No Hit ATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATAC 2162 0.2640120551665523 No Hit GCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAG 2128 0.2598601542064862 No Hit ACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTG 1861 0.22725552019655587 No Hit GATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTT 1826 0.22298150450237025 No Hit CCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATA 1816 0.22176035716117434 No Hit CAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGT 1778 0.21711999726462994 No Hit GTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGC 1590 0.19416242725014715 No Hit GCTTTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC 1567 0.19135378836539657 No Hit TGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGT 1453 0.1774327086757634 No Hit CTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCT 1373 0.16766352994619624 No Hit AAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTAC 1171 0.14299635365403918 No Hit ATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGT 1113 0.135913699075103 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1095 0.1337156338609504 No Hit AGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAG 1090 0.13310506019035245 No Hit GCTTTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGT 1080 0.13188391284915654 No Hit CACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACT 1072 0.13090699497619984 No Hit GCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAAC 1056 0.1289531592302864 No Hit AACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACG 1050 0.12822047082556887 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 991 0.12101570151251309 No Hit GTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTT 969 0.11832917736188213 No Hit ATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCA 914 0.1116128669853047 No Hit AGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTA 911 0.11124652278294594 No Hit GCTTATACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT 842 0.10282060612869429 No Hit TTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGA 830 0.1013552293192592 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 1.221147341195894E-4 0.0 3 0.0 0.0 0.0 1.221147341195894E-4 0.0 4 0.0 0.0 0.0 1.221147341195894E-4 0.0 5 0.0 0.0 0.0 1.221147341195894E-4 0.0 6 0.0 0.0 0.0 1.221147341195894E-4 0.0 7 0.0 0.0 0.0 1.221147341195894E-4 0.0 8 0.0 0.0 0.0 1.221147341195894E-4 0.0 9 0.0 0.0 0.0 2.442294682391788E-4 0.0 10-11 0.0 0.0 0.0 2.442294682391788E-4 0.0 12-13 0.0 0.0 0.0 3.663442023587682E-4 0.0 14-15 0.0 0.0 0.0 4.274015694185629E-4 0.0 16-17 0.0 0.0 0.0 7.326884047175364E-4 0.0 18-19 0.0 0.0 0.0 9.158605058969206E-4 0.0 20-21 0.0 0.0 0.0 0.0012822047082556888 0.0 22-23 0.0 0.0 0.0 0.0025644094165113776 0.0 24-25 0.0 0.0 0.0 0.005128818833022755 0.0 26-27 0.0 0.0 0.0 0.00830380192013208 0.0 28-29 0.0 0.0 0.0 0.02362920105214055 0.0 30-31 0.0 0.0 0.0 0.05769921187150599 0.0 32-33 0.0 0.0 0.0 0.1094758591382119 0.0 34-35 0.0 0.0 0.0 0.18872832158182543 0.0 36-37 0.0 0.0 0.0 0.29741043494826 0.0 38-39 0.0 0.0 0.0 0.4320419293151073 0.0 40-41 0.0 0.0 0.0 0.5791901839292125 0.0 42-43 0.0 0.0 0.0 0.7435566160541798 0.0 44-45 0.0 0.0 0.0 0.9242253651841124 0.0 46-47 0.0 0.0 0.0 1.1508703117100705 0.0 48-49 0.0 0.0 0.0 1.3769046845654302 0.0 50-51 0.0 0.0 0.0 1.6004967627383984 0.0 52-53 0.0 0.0 0.0 1.8084581549440593 0.0 54-55 0.0 0.0 0.0 2.0412088381759967 0.0 56-57 0.0 0.0 0.0 2.3449081819314155 0.0 58-59 0.0 0.0 0.0 2.662284375908228 0.0 60-61 0.0 0.0 0.0 3.0767417835101147 0.0 62-63 0.0 0.0 0.0 3.456091205052619 0.0 64-65 0.0 0.0 0.0 3.8068657788111397 0.0 66-67 0.0 1.221147341195894E-4 0.0 4.22596354630957 0.0 68-69 0.0 1.221147341195894E-4 0.0 4.695189412164092 0.0 70-71 0.0 1.221147341195894E-4 0.0 5.2235188093324965 0.0 72-73 0.0 1.221147341195894E-4 0.0 5.76076258209163 0.0 74-75 0.0 1.221147341195894E-4 0.0 6.3662684912236145 0.0 76-77 0.0 1.221147341195894E-4 0.0 6.906748304436917 0.0 78-79 0.0 1.221147341195894E-4 0.0 7.633392029815534 0.0 80-81 0.0 1.221147341195894E-4 0.0 8.387389455636939 0.0 82-83 0.0 1.221147341195894E-4 0.0 9.057494059118184 0.0 84-85 0.0 1.221147341195894E-4 0.0 9.949053732925307 0.0 86-87 0.0 1.221147341195894E-4 0.0 10.887749694102592 0.0 88-89 0.0 1.221147341195894E-4 0.0 11.721304869202909 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TCGCCGC 35 4.2723914E-6 40.768047 94-95 CTTTGCG 930 0.0 32.07417 2 CATTCCG 90 2.4646582E-5 31.566986 9 TTTGCGT 970 0.0 30.751526 3 GCTTTGC 1000 0.0 30.341398 1 TTGCGTT 1030 0.0 28.960173 4 GTCCTAC 250 0.0 28.445063 1 GTACAGG 125 7.4990166E-6 26.548725 1 CGCAGAA 290 0.0 26.124403 9 AGTGGAC 115 1.309031E-4 24.704597 8 GTGTAGC 155 1.4336856E-6 24.46887 1 CGTAACG 70 1.7632428E-5 23.779171 56-57 GTATGGT 105 0.0022204393 22.575447 1 TCAGGCC 105 0.0022370764 22.546469 3 TAACGGA 75 3.0034587E-5 22.19594 58-59 TCTCGGG 75 3.0034587E-5 22.19594 94-95 GTAACGG 75 3.0055953E-5 22.193893 56-57 GAGTACT 2890 0.0 22.037489 12-13 GTAAGCG 120 5.842594E-9 21.799582 94-95 CCGTATC 55 0.0028559237 21.619421 94-95 >>END_MODULE