##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938875_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 701191 Sequences flagged as poor quality 0 Sequence length 101 %GC 38 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.5628865744141 34.0 31.0 34.0 31.0 34.0 2 32.67766842415262 34.0 31.0 34.0 31.0 34.0 3 32.72033440246666 34.0 31.0 34.0 31.0 34.0 4 36.14498189508993 37.0 37.0 37.0 35.0 37.0 5 36.18889860252057 37.0 37.0 37.0 35.0 37.0 6 36.32869931302598 37.0 37.0 37.0 35.0 37.0 7 36.314861428626436 37.0 37.0 37.0 35.0 37.0 8 36.32767106252077 37.0 37.0 37.0 35.0 37.0 9 38.062797440355055 39.0 39.0 39.0 37.0 39.0 10-11 38.00654529222423 39.0 38.5 39.0 37.0 39.0 12-13 37.975407556571604 39.0 38.0 39.0 37.0 39.0 14-15 39.26226163769928 41.0 39.0 41.0 37.0 41.0 16-17 39.21357590157318 40.0 39.0 41.0 37.0 41.0 18-19 39.16369719520074 40.0 39.0 41.0 36.0 41.0 20-21 39.24529336514587 41.0 39.0 41.0 36.0 41.0 22-23 39.21569158759881 41.0 39.0 41.0 36.0 41.0 24-25 39.12854414845599 41.0 39.0 41.0 35.5 41.0 26-27 38.96293877131909 40.5 38.5 41.0 35.0 41.0 28-29 38.872429195468854 40.0 38.0 41.0 35.0 41.0 30-31 38.7638532154577 40.0 38.0 41.0 35.0 41.0 32-33 38.53173600345697 40.0 38.0 41.0 35.0 41.0 34-35 38.22869303798822 40.0 38.0 41.0 35.0 41.0 36-37 37.92229649268174 40.0 37.5 41.0 34.5 41.0 38-39 37.68951897557156 40.0 37.0 41.0 34.5 41.0 40-41 37.683587638746076 40.0 37.0 41.0 34.5 41.0 42-43 37.513061348477095 40.0 37.0 41.0 34.0 41.0 44-45 37.39568034957665 40.0 37.0 41.0 33.0 41.0 46-47 37.12141342373191 40.0 37.0 41.0 33.0 41.0 48-49 36.833098970180735 40.0 37.0 41.0 33.0 41.0 50-51 35.721329851638146 38.5 35.5 40.0 30.0 40.5 52-53 35.91994549274021 38.5 35.5 40.0 31.0 40.5 54-55 36.50843350813116 40.0 37.0 41.0 32.0 41.0 56-57 36.43772238947733 40.0 36.5 41.0 31.5 41.0 58-59 36.31177525096586 40.0 36.0 41.0 31.0 41.0 60-61 35.92739424778698 39.0 35.0 41.0 31.0 41.0 62-63 35.66009332692519 39.0 35.0 40.0 30.0 41.0 64-65 35.29019197337102 38.5 35.0 40.0 29.0 41.0 66-67 34.76071141814427 37.5 35.0 39.5 28.0 41.0 68-69 34.31098516666643 37.0 35.0 39.0 27.5 41.0 70-71 33.69640155107524 36.0 34.0 38.5 26.0 40.0 72-73 33.199652448476954 36.0 34.0 37.5 26.0 40.0 74-75 32.768212940553994 35.0 34.0 37.0 26.0 39.0 76-77 32.340765925404064 35.0 33.0 36.5 25.5 39.0 78-79 31.916527736379958 35.0 33.0 36.0 23.0 38.5 80-81 31.45307697902569 35.0 32.0 36.0 20.5 37.0 82-83 30.96167520689798 35.0 31.5 35.0 18.5 37.0 84-85 30.53687939520045 34.5 31.0 35.0 12.5 36.5 86-87 30.31063290886506 34.0 31.0 35.0 6.5 36.0 88-89 29.800676991005304 34.0 31.0 35.0 2.0 36.0 90-91 29.33029873458159 34.0 30.0 35.0 2.0 35.0 92-93 28.914767160445585 34.0 29.5 35.0 2.0 35.0 94-95 28.413470081618275 34.0 29.0 35.0 2.0 35.0 96-97 27.782635544380916 34.0 29.0 35.0 2.0 35.0 98-99 27.157177573585514 34.0 27.5 35.0 2.0 35.0 100-101 23.996950902107983 30.0 13.5 33.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.18398276677807957 1101 2 0.2842943443063959 1101 3 0.2132822575392197 1101 4 0.1079319472800293 1101 5 0.1737672099849661 1101 6 0.2237912622653937 1101 7 0.20675215458932428 1101 8 0.24451072656284367 1101 9 0.38642169617929767 1101 10-11 0.25207172315053583 1101 12-13 0.2011875369691225 1101 14-15 0.2686062298400529 1101 16-17 0.24931947153321943 1101 18-19 0.3239579976438378 1101 20-21 0.415600301786462 1101 22-23 0.4444886602819551 1101 24-25 0.4236038958040851 1101 26-27 0.37228658962417427 1101 28-29 0.23704509248653238 1101 30-31 0.14589866727971668 1101 32-33 0.18276085000268694 1101 34-35 0.2050321351475901 1101 36-37 0.2558981260575308 1101 38-39 0.23535595710561807 1101 40-41 0.22266622076346465 1101 42-43 0.2019024339842872 1101 44-45 0.2832702759455259 1101 46-47 -0.03324327129042359 1101 48-49 -0.040512140015827924 1101 50-51 0.037910425123335756 1101 52-53 0.18295401567723246 1101 54-55 0.41001248442339744 1101 56-57 0.44138661376971555 1101 58-59 0.4889198081718149 1101 60-61 0.3816352081561618 1101 62-63 0.42540922147889404 1101 64-65 0.3945226950322507 1101 66-67 0.34250414147037844 1101 68-69 0.2706492575760038 1101 70-71 0.04585594648948188 1101 72-73 0.0656042723048671 1101 74-75 0.16534099051508377 1101 76-77 0.03783772984851197 1101 78-79 -0.171619121018054 1101 80-81 -0.2382427154271589 1101 82-83 0.001240441552710081 1101 84-85 -0.272744071075536 1101 86-87 -0.2165373459669162 1101 88-89 -0.25591831776671725 1101 90-91 -0.25828146786451 1101 92-93 -0.33740342263570255 1101 94-95 -0.30825198663546516 1101 96-97 -0.5040248855761895 1101 98-99 -0.5469274839339278 1101 100-101 -0.20230823275546328 1102 1 0.23165444376009958 1102 2 0.23610689956722553 1102 3 0.10551135666486999 1102 4 0.08143130871042814 1102 5 0.22320723356292405 1102 6 0.18760155797223632 1102 7 0.14799147082864295 1102 8 0.17970819193272547 1102 9 0.2457890210638638 1102 10-11 0.2974777060565259 1102 12-13 0.11121676380869161 1102 14-15 0.2546559400276962 1102 16-17 0.24239344736581359 1102 18-19 0.298273991356389 1102 20-21 0.2808436950471034 1102 22-23 0.2748379087346464 1102 24-25 0.39228942483444484 1102 26-27 0.266652440886574 1102 28-29 0.28082395092057055 1102 30-31 0.3273261159485443 1102 32-33 0.29463947265717394 1102 34-35 0.3129135576841904 1102 36-37 0.3688635450574296 1102 38-39 0.43748165707614106 1102 40-41 0.5061481443315969 1102 42-43 0.5689571544872791 1102 44-45 0.7127267549709657 1102 46-47 0.776616489000368 1102 48-49 0.6585557939658955 1102 50-51 0.5963027033431985 1102 52-53 0.7127518838371714 1102 54-55 0.6087427133260448 1102 56-57 0.5907136596484932 1102 58-59 0.546298971154421 1102 60-61 0.5002188116535606 1102 62-63 0.40490131303995014 1102 64-65 0.4893195807774191 1102 66-67 0.510429527068176 1102 68-69 0.525137665081651 1102 70-71 0.5482628626894979 1102 72-73 0.3563683453965325 1102 74-75 0.2815422381818493 1102 76-77 0.19081199058138765 1102 78-79 0.14495790030035138 1102 80-81 0.2501402298523381 1102 82-83 0.5232158320625864 1102 84-85 0.3591880931842155 1102 86-87 0.3878073833778153 1102 88-89 0.21836697548409134 1102 90-91 0.3981251044903402 1102 92-93 0.21075946173408155 1102 94-95 0.1890525128242082 1102 96-97 0.3528864023695846 1102 98-99 0.21595442981005775 1102 100-101 0.1363915412891359 1103 1 0.029375241480899206 1103 2 0.0922322556925863 1103 3 0.08271933387284491 1103 4 0.05294128022040212 1103 5 0.02235253270822568 1103 6 0.06366993404061105 1103 7 0.004116826953996622 1103 8 -0.02684451461998094 1103 9 0.049207824482664364 1103 10-11 -0.03443112585230779 1103 12-13 0.11833927093119456 1103 14-15 0.1136302990020539 1103 16-17 0.17259287756524344 1103 18-19 0.16152185460425272 1103 20-21 0.23810865231205725 1103 22-23 0.20646184035857829 1103 24-25 0.1237709063159187 1103 26-27 0.19400286823699986 1103 28-29 0.008031928128545474 1103 30-31 0.1371551757776004 1103 32-33 0.12441155242925106 1103 34-35 0.04795629272692281 1103 36-37 0.14521682141070613 1103 38-39 0.30286627246075426 1103 40-41 0.21626210444555483 1103 42-43 0.16154974707987435 1103 44-45 0.2832395754837833 1103 46-47 0.20752816991204526 1103 48-49 0.403570038980142 1103 50-51 0.32066167770216936 1103 52-53 0.43426185534714534 1103 54-55 0.4142982688815948 1103 56-57 0.3585199274547577 1103 58-59 0.28490295975841207 1103 60-61 0.39765470908945844 1103 62-63 0.5309696891083249 1103 64-65 0.4729378143956495 1103 66-67 0.4149879316265839 1103 68-69 0.5778442177882042 1103 70-71 0.39797796240459604 1103 72-73 0.37346236249054243 1103 74-75 0.19892115556076817 1103 76-77 0.41018520995461216 1103 78-79 0.2824222877647351 1103 80-81 0.16467014438224936 1103 82-83 0.26609332494007987 1103 84-85 0.4147436487397691 1103 86-87 0.32014356571399816 1103 88-89 0.2076832148003298 1103 90-91 -0.11327090690567232 1103 92-93 -0.34337159239697357 1103 94-95 -0.3024004786287833 1103 96-97 -0.2197631702799896 1103 98-99 -0.10170938785375938 1103 100-101 -0.07443466953707656 1104 1 0.1020553828305708 1104 2 0.12991123543164917 1104 3 0.20065337884551582 1104 4 0.17974636196110083 1104 5 0.044802186059165194 1104 6 -0.030432752336750468 1104 7 0.05278219750778135 1104 8 0.04458842376574523 1104 9 0.023958078202873878 1104 10-11 0.09783352572564752 1104 12-13 0.07231809293576674 1104 14-15 0.10602174954801313 1104 16-17 0.1664424551058019 1104 18-19 0.2738648843292495 1104 20-21 0.20737488123493364 1104 22-23 0.22839793954789656 1104 24-25 0.25230006274207284 1104 26-27 0.14327716729670925 1104 28-29 0.19572374672164727 1104 30-31 0.270621177269355 1104 32-33 0.22678001531273395 1104 34-35 0.39761636878184703 1104 36-37 0.4031096597756445 1104 38-39 0.38298989228394476 1104 40-41 0.21015142129625275 1104 42-43 0.3490214710322874 1104 44-45 0.3204508541113782 1104 46-47 0.24180791020037162 1104 48-49 0.23791825315668547 1104 50-51 0.22781842176878087 1104 52-53 0.3163002985443839 1104 54-55 0.4096609970769407 1104 56-57 0.3625794508275817 1104 58-59 0.3446280860071411 1104 60-61 0.24375658845313808 1104 62-63 -0.03553251795182888 1104 64-65 0.002568055399279956 1104 66-67 0.08598312622692816 1104 68-69 0.3176098169958621 1104 70-71 0.3174508357401322 1104 72-73 0.45660311301327283 1104 74-75 0.25802573719753497 1104 76-77 0.26951722272009704 1104 78-79 0.0835576092863235 1104 80-81 0.42616703248858556 1104 82-83 0.5566243643981572 1104 84-85 0.284206128717269 1104 86-87 0.17455707463394177 1104 88-89 0.23903753048940857 1104 90-91 0.25945631024214677 1104 92-93 0.2382835193010564 1104 94-95 0.30734726845801674 1104 96-97 0.3111051030775549 1104 98-99 0.26175857003479663 1104 100-101 0.8686983776732262 1105 1 0.08059615536275544 1105 2 0.029768173915201146 1105 3 0.05473063206439832 1105 4 0.0466991516606754 1105 5 0.05767772253985015 1105 6 0.09260015181203585 1105 7 0.07853327046915837 1105 8 0.11611918199178461 1105 9 0.03111115402548137 1105 10-11 0.19654597207757973 1105 12-13 0.04585171239213537 1105 14-15 0.17540658502726814 1105 16-17 0.1843251446623171 1105 18-19 0.2602740402663031 1105 20-21 0.2710372560561112 1105 22-23 0.19549379076392626 1105 24-25 0.30594813141160415 1105 26-27 0.2047936193711024 1105 28-29 0.10058783828102236 1105 30-31 0.30781717154689403 1105 32-33 0.2418014745402033 1105 34-35 0.26385385089916014 1105 36-37 0.17349592587007123 1105 38-39 0.26711006395776593 1105 40-41 0.19155342415748322 1105 42-43 0.16232619206233068 1105 44-45 0.13304026755915999 1105 46-47 0.05940447672397653 1105 48-49 0.006158596504327818 1105 50-51 -0.18705997594222623 1105 52-53 -0.2166038502395935 1105 54-55 -0.11537476830216775 1105 56-57 -0.29979537034551385 1105 58-59 -0.2726823574835535 1105 60-61 -0.27126487077433126 1105 62-63 -0.13281434913923817 1105 64-65 -0.15050776720444503 1105 66-67 0.10171989303665185 1105 68-69 -0.08868488972802169 1105 70-71 -0.39642613135572446 1105 72-73 -0.3323811502199163 1105 74-75 -0.34783978497699053 1105 76-77 -0.35566160417546655 1105 78-79 -0.37280862819579497 1105 80-81 -0.4493694481981123 1105 82-83 -0.21233128653174305 1105 84-85 -0.21293264095368514 1105 86-87 -0.33474192393543944 1105 88-89 -0.6472285639311899 1105 90-91 -0.37072966972923993 1105 92-93 -0.6723030329163961 1105 94-95 -0.576057595633543 1105 96-97 -0.8212267996406162 1105 98-99 -0.6738637475617679 1105 100-101 -0.28151621460145293 1106 1 0.1160887476859287 1106 2 0.0912785640609286 1106 3 0.17612075354544743 1106 4 -0.009591917906021763 1106 5 0.07830646578079836 1106 6 0.12660150968405048 1106 7 0.09587644837463927 1106 8 0.039732818906507816 1106 9 0.10895237998993679 1106 10-11 0.19310279659686813 1106 12-13 0.19438108849843871 1106 14-15 0.15407768902571206 1106 16-17 0.19321193208259757 1106 18-19 0.26596316031673695 1106 20-21 0.21475527938240901 1106 22-23 0.20622643546215613 1106 24-25 0.16870909744056206 1106 26-27 0.12043582009368237 1106 28-29 0.0667986479122149 1106 30-31 0.04663897509191628 1106 32-33 0.1080116779785314 1106 34-35 0.13859924989530015 1106 36-37 0.27228717729462204 1106 38-39 0.2294018367081847 1106 40-41 0.27070099574886086 1106 42-43 0.37823693622469534 1106 44-45 0.5452150450863726 1106 46-47 0.3425238239754975 1106 48-49 0.28362822624172424 1106 50-51 0.12545669436159557 1106 52-53 0.14034978812998844 1106 54-55 0.3019859891964316 1106 56-57 0.3526227329982419 1106 58-59 0.35623667939722026 1106 60-61 0.3025269868430911 1106 62-63 0.3416619002242598 1106 64-65 0.09945889218791137 1106 66-67 0.27014148805132976 1106 68-69 0.15596242726234522 1106 70-71 0.35359508539460194 1106 72-73 0.07793867713635194 1106 74-75 -0.12682489688020127 1106 76-77 -0.19976698474334853 1106 78-79 -0.15964999964653614 1106 80-81 -0.4081725810028445 1106 82-83 -0.14515299308590102 1106 84-85 -0.177076332910719 1106 86-87 -0.06540740246408916 1106 88-89 0.16201466489449246 1106 90-91 -0.022271824381167704 1106 92-93 0.2001964215100216 1106 94-95 0.37849309421394395 1106 96-97 0.27719467413548315 1106 98-99 0.2646349551854996 1106 100-101 0.6141246524968267 1107 1 0.08774923118536293 1107 2 0.07697847434438643 1107 3 0.05473063206439832 1107 4 0.17831574679658502 1107 5 0.05767772253985015 1107 6 0.09116953664751293 1107 7 0.027031124546411434 1107 8 0.0417271934367065 1107 9 0.09835006675795199 1107 10-11 0.12644582901606327 1107 12-13 0.13955700566824447 1107 14-15 0.15108612723041404 1107 16-17 0.022665631071468795 1107 18-19 0.019215385044560662 1107 20-21 0.029978600834361657 1107 22-23 0.015236280034308436 1107 24-25 0.009095484773546048 1107 26-27 -0.01623642354735466 1107 28-29 -0.06321759805660321 1107 30-31 0.15259542619639177 1107 32-33 -0.048613403857508786 1107 34-35 -0.15889293021672302 1107 36-37 -0.17271294394394943 1107 38-39 -0.08196003618530057 1107 40-41 -0.061665459962689795 1107 42-43 0.011396292205390068 1107 44-45 0.035758436371743585 1107 46-47 -0.0013966677681551687 1107 48-49 0.1957151058033233 1107 50-51 0.13411312849267887 1107 52-53 0.13675809539702755 1107 54-55 0.1650258039438981 1107 56-57 0.22309447228681023 1107 58-59 0.2494921775665162 1107 60-61 0.30813427085656997 1107 62-63 0.3106763518621918 1107 64-65 0.2379042499629378 1107 66-67 0.17968841950303727 1107 68-69 0.2482249815166071 1107 70-71 0.17295870412353764 1107 72-73 0.08965032331370537 1107 74-75 0.01911300472257693 1107 76-77 0.08139132858561737 1107 78-79 0.05065346050234254 1107 80-81 0.1357521540908735 1107 82-83 0.23402064479872564 1107 84-85 0.2119600629089753 1107 86-87 0.2589633693406661 1107 88-89 0.10956685810028333 1107 90-91 0.5169670398558814 1107 92-93 0.21968552216228687 1107 94-95 0.06485799807175141 1107 96-97 0.4920779213894271 1107 98-99 0.4649059233967421 1107 100-101 0.7034623261710777 1108 1 -0.022048450567794475 1108 2 -0.09322002350969427 1108 3 -0.0360646599111476 1108 4 -0.10675261384849222 1108 5 -0.037962152606461075 1108 6 -0.03407838245770023 1108 7 -0.10053951207734002 1108 8 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-0.49803934796005933 2316 80-81 -0.5246548081005926 2316 82-83 -0.4575108460325126 2316 84-85 -0.4386249019972013 2316 86-87 -0.3791143967018584 2316 88-89 -0.3904280543635714 2316 90-91 -0.569428481484298 2316 92-93 -0.8071349508971721 2316 94-95 -0.5877080534100436 2316 96-97 -0.9893586767176004 2316 98-99 -1.1188094839012237 2316 100-101 -0.7556817060472696 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 2829.0 3 481.0 4 390.0 5 346.0 6 350.0 7 429.0 8 433.0 9 472.0 10 571.0 11 800.0 12 1522.0 13 3081.0 14 2631.0 15 2806.0 16 4243.0 17 7238.0 18 5782.0 19 2711.0 20 2275.0 21 2124.0 22 2231.0 23 2538.0 24 2891.0 25 3297.0 26 3958.0 27 4678.0 28 6056.0 29 7332.0 30 9502.0 31 12507.0 32 17180.0 33 24968.0 34 40118.0 35 77922.0 36 102300.0 37 164121.0 38 156726.0 39 21351.0 40 1.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.8563129728099 21.7965453690187 16.25944190023291 18.087699757938488 2 13.514080497087999 25.231154767732093 36.46829479921395 24.786469935965954 3 23.126074887069954 27.426907679242994 33.88029772141782 15.566719712269233 4 10.20889949530704 18.094156066592067 44.28966178720684 27.40728265089406 5 9.099395771686487 41.67904956442176 37.27530571840077 11.946248945490984 6 23.203182294208123 39.32895596665896 18.59082465136586 18.877037087767057 7 20.100275228965103 37.48150515572766 24.64062573569173 17.77759387961551 8 21.278165236051503 29.16842639941558 22.91999589078623 26.633412473746688 9 20.14576751964066 22.366382151754603 29.23897255016109 28.248877778443642 10-11 22.462681456158023 25.40157920720032 29.105967257276085 23.029772079365575 12-13 27.703679289567667 26.195622862475016 27.967708658822122 18.132989189135202 14-15 22.848516929504036 27.69430299467667 32.602867550226364 16.854312525592935 16-17 16.33133654787969 31.14607209686728 32.86955690481847 19.65303445043456 18-19 15.4142234100312 34.04116728391671 32.77201749085184 17.77259181520026 20-21 18.5594996710991 29.153176497783306 29.627692742348593 22.659631088768997 22-23 16.62391372444367 28.643067993946325 32.339847716279344 22.39317056533066 24-25 21.128000787793763 29.15864482346712 30.38222269160015 19.331131697138968 26-27 19.520420140784267 25.852429830851946 36.85643643836496 17.770713589998824 28-29 16.09921082299887 32.02010760207212 33.77834543975568 18.10233613517332 30-31 16.928714741952334 29.430962295329945 32.45260159654776 21.18772136616997 32-33 17.787669527410127 29.41381276054728 33.79981835265982 18.99869935938277 34-35 20.92386486471029 27.692854324066186 31.62050249646834 19.762778314755185 36-37 19.234194760415594 26.705456659449922 36.148292610284436 17.91205596985005 38-39 19.00427614107973 29.220559835196063 33.78802823656065 17.98713578716356 40-41 17.12095729529726 30.87205765528495 34.345616809922205 17.661368239495587 42-43 16.342320531056696 31.191276765267418 30.409696237346207 22.056706466329686 44-45 17.71454835204014 28.801819564180875 30.894576100823446 22.589055982955546 46-47 20.585508611747255 30.56151928130801 31.02756088429068 17.82541122265406 48-49 19.123312858397888 28.910971620293168 31.952569939720775 20.013145581588166 50-51 15.313272567287859 30.40832004627976 35.78397434566482 18.494433040767568 52-53 17.542857306504917 32.515851332542915 30.316496841188833 19.624794519763334 54-55 15.702546788486401 28.572870624519442 33.324670787461216 22.399911799532937 56-57 19.75152051777415 28.72338064946527 30.272780566576742 21.252318266183842 58-59 21.679292613016234 28.264132173440064 30.711860039863875 19.344715173679823 60-61 18.95425538003663 29.026084592490843 34.39188415750915 17.62777586996337 62-63 17.930768883865326 29.670580605351716 33.2959906884385 19.102659822344457 64-65 17.034162980486276 32.672923507401244 31.800913323089837 18.492000189022654 66-67 18.05389869005389 29.462831872347923 29.358359839203292 23.124909598394897 68-69 16.045116012603838 29.86608421655686 32.41721569750788 21.671584073331424 70-71 20.412907106642606 29.69357795537223 29.327146171693734 20.566368766291426 72-73 18.653208875497292 26.62692150162828 34.4147493419564 20.305120280918025 74-75 16.69077389945709 32.90178999783751 30.900698727854163 19.506737374851237 76-77 17.84771585233061 28.672852609099365 30.962548583464045 22.51688295510598 78-79 18.021816297299484 28.487597608925796 33.21956809772739 20.271017996047327 80-81 20.229881108073613 28.877874677071947 30.562648221202405 20.329595993652035 82-83 17.901190377329858 28.24597599773207 34.101862165931216 19.750971459006863 84-85 18.0854978401465 29.10046432912817 31.713765361304763 21.10027246942057 86-87 17.69807225327591 30.630540983231008 31.96710631815266 19.70428044534042 88-89 18.364331323278524 29.135938935188797 29.77768645865475 22.722043282877927 90-91 18.02945337694251 27.163465669230902 31.601162211237188 23.2059187425894 92-93 20.020893808186536 29.887955342528137 31.835705069362003 18.255445779923328 94-95 18.745153023045653 28.277702151610217 31.712458887968324 21.264685937375805 96-97 15.656737791780037 28.588285837032796 34.01009594730059 21.74488042388658 98-99 18.325341001770795 29.82577743534589 29.552174040687497 22.296707522195817 100-101 17.768039764693587 26.958864332264 32.152100096147166 23.120995806895248 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 17333.0 1 9678.0 2 1940.5 3 1624.0 4 1147.5 5 1150.5 6 2156.0 7 3881.0 8 5250.0 9 5209.5 10 3625.0 11 2231.5 12 1983.5 13 1859.5 14 1649.5 15 1529.0 16 1579.5 17 1583.0 18 1428.5 19 1416.5 20 1623.0 21 1911.5 22 2160.0 23 2365.0 24 2535.5 25 3134.0 26 4816.0 27 6490.5 28 6650.0 29 6971.5 30 8762.5 31 10575.5 32 13130.0 33 16880.5 34 20912.5 35 23052.5 36 25408.5 37 28465.0 38 26257.0 39 22035.0 40 19170.5 41 16894.5 42 15079.0 43 14462.0 44 14733.0 45 14772.5 46 18900.0 47 41067.5 48 94826.0 49 91391.5 50 35549.5 51 12796.5 52 5253.0 53 2947.0 54 2036.0 55 1459.5 56 1118.0 57 895.5 58 760.5 59 685.5 60 576.0 61 432.0 62 308.0 63 263.5 64 185.0 65 124.0 66 108.0 67 107.5 68 114.5 69 94.0 70 68.0 71 57.5 72 47.5 73 35.0 74 28.5 75 26.0 76 21.5 77 17.0 78 14.5 79 13.0 80 9.5 81 5.5 82 3.0 83 2.5 84 3.0 85 2.0 86 1.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.5 95 0.5 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.25413902916609027 2 0.0670288124063201 3 0.07644136904210122 4 0.08086241837102871 5 0.08956190253440219 6 0.04463833677271956 7 0.04563663823409028 8 0.04663493969546101 9 0.04977245857405472 10-11 0.047490626662350204 12-13 0.045921867223053345 14-15 0.044780951267201094 16-17 0.044852258514441856 18-19 0.05241082672196305 20-21 0.053052591947129954 22-23 0.06553136021426402 24-25 0.07130724724076606 26-27 0.06752796313700546 28-29 0.0657452819559863 30-31 0.06118161813257729 32-33 0.054550044139186044 34-35 0.2571339335502024 36-37 0.40145980196551295 38-39 0.37992501329880163 40-41 0.3645226478947961 42-43 0.38997933515974964 44-45 0.39675352364762245 46-47 0.39297423954386185 48-49 0.40752091798097806 50-51 0.40352771213549515 52-53 0.4031711758992913 54-55 0.39675352364762245 56-57 0.3797110915570793 58-59 0.3655922566034076 60-61 0.32958209674682076 62-63 0.38570090032530363 64-65 0.40816268320614496 66-67 0.41614909489711077 68-69 0.42655995299426264 70-71 0.423707663104632 72-73 0.414865564446777 74-75 0.4167195528750369 76-77 0.3963969874114186 78-79 0.3817076944798208 80-81 0.384987827852896 82-83 0.3931881612855841 84-85 0.39418646274695485 86-87 0.39347339027454714 88-89 0.39725267437830775 90-91 0.40887575567855267 92-93 0.4132968050074801 94-95 0.4202136079898344 96-97 0.4122985035461094 98-99 0.41607778764987 100-101 0.3968248308948632 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 701191.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 16.648459896224065 #Duplication Level Percentage of deduplicated Percentage of total 1 74.08840653045294 12.334578648973912 2 10.066535075476878 3.3518461099601953 3 3.8883219096532704 1.9420371412941555 4 2.1707462496895564 1.4455832753134148 5 1.3674320703648475 1.1382818992139907 6 0.9902551761446176 0.9891734152243179 7 0.7777846956176141 0.9064242119020685 8 0.6047491697525726 0.8054513839920405 9 0.49167928761368973 0.7367132611476479 >10 4.27264036699402 15.26975399531687 >50 0.7161041146046895 8.421003695086023 >100 0.5002449795803094 15.689021532851635 >500 0.02312776446689787 2.6241302115711758 >1k 0.02655409994347533 10.643692324918552 >5k 0.01113559029887675 12.11526450323511 >10k+ 0.0042829193457218275 11.587044389998892 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 22806 3.252466161145822 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 21298 3.0374035034676714 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 13861 1.9767795080085169 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 11978 1.7082364148997922 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 11308 1.6126847035971652 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 9473 1.350987106223554 No Hit TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT 8180 1.1665865648589329 No Hit ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC 7895 1.1259414339316962 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6787 0.9679245740461586 No Hit GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT 6432 0.9172964285052148 No Hit GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 6217 0.8866343121916852 No Hit ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC 6204 0.8847803237634254 No Hit TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC 6014 0.8576835698119343 No Hit GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA 5663 0.8076258822489165 No Hit CCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCAC 5619 0.8013508444917291 No Hit GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG 5615 0.800780386513803 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5551 0.7916530588669849 No Hit GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA 5305 0.7565698932245281 No Hit TGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGAT 4994 0.7122167854407715 No Hit GATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATA 4958 0.7070826636394363 No Hit GTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTG 4452 0.6349197294317811 No Hit GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT 4416 0.6297856076304459 No Hit GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA 4002 0.5707432069150916 No Hit TACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACC 3849 0.5489231892594172 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3716 0.5299554614933734 No Hit AAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGC 3304 0.4711982897669822 No Hit ATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATAC 3256 0.46435279403186863 No Hit ACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTG 3044 0.43411852120178385 No Hit CTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCA 2720 0.3879114249897674 No Hit CTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTG 2598 0.37051245666302046 No Hit GCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCT 2441 0.34812198102941994 No Hit GCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAG 2165 0.3087603805525171 No Hit CTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCT 2143 0.3056228616739234 No Hit GCTTTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC 2088 0.2977790644774391 No Hit TGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCAC 2063 0.29421370211540077 No Hit CAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGT 1890 0.26954139457009574 No Hit CACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACT 1605 0.22889626364285906 No Hit GTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTG 1603 0.22861103465389604 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1529 0.21805756206226262 No Hit GGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGT 1463 0.2086450054264815 No Hit ATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTG 1323 0.18867897619906704 No Hit TATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGG 1321 0.18839374721010396 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1177 0.16785726000476334 No Hit AACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACG 1138 0.16229529471998358 No Hit ACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCA 1125 0.16044130629172365 No Hit TTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGA 1109 0.15815947438001912 No Hit AGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAG 1071 0.15274012358972092 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1054 0.15031567718353486 No Hit ACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGT 1019 0.14532416987668126 No Hit GTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCA 963 0.13733775818571545 No Hit CAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAG 930 0.1326314798678249 No Hit AGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTA 869 0.12393199570445142 No Hit CGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTT 867 0.12364676671548837 No Hit GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 858 0.12236323626515458 No Hit ATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCA 852 0.12150754929826538 No Hit CCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATA 806 0.1149472825521149 No Hit GTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGC 791 0.11280806513489193 No Hit GATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTT 742 0.10581995490529683 No Hit TGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGT 723 0.10311027951014774 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 1.4261449448153214E-4 0.0 12-13 0.0 0.0 0.0 2.852289889630643E-4 0.0 14-15 0.0 0.0 0.0 3.5653623620383033E-4 0.0 16-17 0.0 0.0 0.0 4.991507306853624E-4 0.0 18-19 0.0 0.0 0.0 6.417652251668945E-4 0.0 20-21 0.0 0.0 0.0 0.001140915955852257 0.0 22-23 0.0 0.0 0.0 0.0019252956755006839 0.0 24-25 0.0 0.0 0.0 0.002994904384112175 0.0 26-27 0.0 0.0 0.0 0.004421049328927496 0.0 28-29 0.0 0.0 0.0 0.012550075514374828 0.0 30-31 0.0 0.0 0.0 0.031874339516622434 0.0 32-33 0.0 0.0 0.0 0.07380300089419288 0.0 34-35 0.0 0.0 0.0 0.13790821616364157 0.0 36-37 0.0 0.0 0.0 0.21755841133157727 0.0 38-39 0.0 0.0 0.0 0.3269437285989124 0.0 40-41 0.0 0.0 0.0 0.4436023850848057 0.0 42-43 0.0 0.0 0.0 0.5749503345022968 0.0 44-45 0.0 0.0 0.0 0.7301149044982038 0.0 46-47 0.0 0.0 0.0 0.9270655213771997 0.0 48-49 0.0 0.0 0.0 1.1050484104901517 0.0 50-51 0.0 0.0 0.0 1.303068636077759 0.0 52-53 0.0 0.0 0.0 1.4860430324975649 0.0 54-55 0.0 0.0 0.0 1.705811968493606 0.0 56-57 0.0 0.0 0.0 2.0228439897260517 0.0 58-59 0.0 0.0 0.0 2.315988083132841 0.0 60-61 0.0 0.0 0.0 2.6774445193962846 0.0 62-63 0.0 0.0 0.0 3.036476509253541 0.0 64-65 0.0 0.0 0.0 3.377681687300607 0.0 66-67 0.0 0.0 0.0 3.7909071850608465 0.0 68-69 0.0 0.0 0.0 4.273158098150148 0.0 70-71 0.0 0.0 0.0 4.8030422523962795 0.0 72-73 0.0 0.0 0.0 5.313744757134646 0.0 74-75 0.0 0.0 0.0 5.918216291994621 0.0 76-77 0.0 0.0 0.0 6.447672602757309 0.0 78-79 0.0 0.0 0.0 7.215223812056914 0.0 80-81 0.0 0.0 0.0 8.026771592904073 0.0 82-83 0.0 0.0 0.0 8.695419650280737 0.0 84-85 0.0 0.0 0.0 9.504329062979988 0.0 86-87 0.0 0.0 0.0 10.34511281519586 0.0 88-89 0.0 0.0 0.0 11.208643579281537 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTAAGCG 60 0.0 47.55681 94-95 TAGGCAT 160 0.0 44.42221 5 GTGTACG 45 9.647264E-4 42.20016 1 CATTCCG 170 0.0 41.797207 9 CCTTTAG 190 0.0 39.9791 1 TTAGGCA 185 0.0 38.41921 4 GATCCTC 65 1.349207E-4 36.438587 6 GGCATTC 200 0.0 35.527626 7 GACGTGA 55 0.0026137885 34.45103 7 CTTTAGG 235 0.0 32.261234 2 CTTATGT 60 0.0039975946 31.589127 2 TAGTCGA 60 0.0039975946 31.589127 5 GCTTTGC 775 0.0 30.016567 1 GGACACC 95 3.5547113E-5 29.918 6 GGATATG 240 0.0 29.671991 1 AGGCATT 240 0.0 29.606354 6 CTAGTCG 65 0.005912946 29.159193 4 TATGTAC 65 0.005912946 29.159193 4 CAGAGTT 65 0.005912946 29.159193 4 AGTCGAA 65 0.0059212004 29.15087 6 >>END_MODULE