##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938874_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 696043 Sequences flagged as poor quality 0 Sequence length 101 %GC 38 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.48778020898134 31.0 31.0 34.0 30.0 34.0 2 31.725637640203264 31.0 31.0 34.0 30.0 34.0 3 31.77378121753972 31.0 31.0 34.0 30.0 34.0 4 35.44939608616134 37.0 35.0 37.0 33.0 37.0 5 35.37336199056667 37.0 35.0 37.0 33.0 37.0 6 35.524773613124474 37.0 35.0 37.0 33.0 37.0 7 35.467922240436295 37.0 35.0 37.0 33.0 37.0 8 35.48364397027195 37.0 35.0 37.0 33.0 37.0 9 36.96040474510914 39.0 37.0 39.0 33.0 39.0 10-11 36.84193074278457 39.0 37.0 39.0 32.5 39.0 12-13 36.8004886192376 39.0 37.0 39.0 32.0 39.0 14-15 37.52957504062249 39.0 36.0 41.0 32.0 41.0 16-17 37.54745827484796 39.0 36.5 40.5 32.5 41.0 18-19 37.77784202987459 39.5 37.0 41.0 32.5 41.0 20-21 37.791533712716024 40.0 37.0 41.0 32.5 41.0 22-23 37.66886600396815 39.0 36.5 41.0 32.0 41.0 24-25 37.44930413781907 39.0 36.0 41.0 32.0 41.0 26-27 37.0695129467576 39.0 35.5 40.5 31.5 41.0 28-29 36.903937687757804 39.0 35.5 40.0 31.0 41.0 30-31 36.78047261447928 39.0 35.0 40.0 31.0 41.0 32-33 36.469224602503004 38.0 35.0 40.0 30.0 41.0 34-35 36.23233622060706 38.0 35.0 40.0 30.0 41.0 36-37 35.97687283687933 38.0 35.0 40.0 30.0 41.0 38-39 35.7162553175594 38.0 35.0 40.0 30.0 41.0 40-41 35.6720224181552 38.0 35.0 40.0 29.5 41.0 42-43 35.44338424493889 38.0 35.0 40.0 28.5 41.0 44-45 35.12445854638291 38.0 34.0 40.0 27.0 41.0 46-47 34.640741304775716 38.0 33.0 40.0 24.5 41.0 48-49 34.152008568436145 38.0 33.0 40.0 23.0 41.0 50-51 33.80185994256102 37.0 33.0 40.0 23.0 41.0 52-53 33.60768443903609 37.0 33.0 40.0 23.0 41.0 54-55 33.407347678232526 37.0 32.5 40.0 22.0 41.0 56-57 32.99301479937303 36.5 31.5 39.5 20.0 40.5 58-59 32.652762832181345 36.0 31.0 39.0 19.0 40.0 60-61 31.85376765515924 35.5 31.0 39.0 14.0 40.0 62-63 31.531449206442705 35.0 30.0 38.0 13.0 40.0 64-65 31.19396071794415 35.0 29.5 38.0 10.5 40.0 66-67 30.511784473085715 34.5 28.5 37.5 8.0 39.0 68-69 29.925798406132955 34.0 28.0 37.0 6.5 39.0 70-71 29.173436842264053 33.5 27.0 36.0 2.0 39.0 72-73 28.450858639480607 33.0 26.0 35.5 2.0 38.0 74-75 27.89027473877332 32.5 26.0 35.0 2.0 37.0 76-77 26.213315843992397 30.5 24.5 33.5 2.0 35.5 78-79 27.15404292550891 32.0 26.0 34.5 2.0 36.0 80-81 27.01834441262968 32.0 26.0 35.0 2.0 36.0 82-83 26.525475437580724 32.0 24.5 34.5 2.0 35.5 84-85 25.904153191684998 31.5 23.5 34.0 2.0 35.0 86-87 25.234551600978676 31.0 20.5 34.0 2.0 35.0 88-89 24.296613140280126 31.0 17.5 34.0 2.0 35.0 90-91 23.469222447463736 30.0 9.0 34.0 2.0 35.0 92-93 22.82423140524364 29.0 3.5 34.0 2.0 35.0 94-95 21.985366564996703 29.0 2.0 34.0 2.0 35.0 96-97 20.71182024673763 29.0 2.0 33.5 2.0 35.0 98-99 19.316484039060803 26.5 2.0 33.0 2.0 35.0 100-101 15.114010197645836 11.0 2.0 28.5 2.0 32.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.01779464912773676 1101 2 0.1111117266118633 1101 3 0.13401204918626064 1101 4 0.014002839141049606 1101 5 0.12121900626165427 1101 6 0.12459089830035452 1101 7 0.12702403624887637 1101 8 0.18411954264982455 1101 9 0.20706020575133266 1101 10-11 0.16035078325271002 1101 12-13 0.10819191030054043 1101 14-15 0.2999240740274587 1101 16-17 0.20005294069563462 1101 18-19 0.2614096042386933 1101 20-21 0.26701276248940076 1101 22-23 0.46712490735230716 1101 24-25 0.5255305414935734 1101 26-27 0.5093572999793281 1101 28-29 0.476643140727802 1101 30-31 0.3518373962644503 1101 32-33 0.5364293336807435 1101 34-35 0.7935013710082615 1101 36-37 0.9681716089546697 1101 38-39 0.8807359076472707 1101 40-41 1.0391201091717548 1101 42-43 0.7544990799676654 1101 44-45 0.8728357151244452 1101 46-47 1.0654082725135723 1101 48-49 0.8768197630773358 1101 50-51 1.3174732611866702 1101 52-53 1.1781390928784532 1101 54-55 1.0772887233174373 1101 56-57 0.802910165130605 1101 58-59 1.4290092892138162 1101 60-61 1.3564780356749573 1101 62-63 1.2743730374789628 1101 64-65 1.0556761172238396 1101 66-67 1.0210759917387442 1101 68-69 0.9060456883200381 1101 70-71 1.3015945028892268 1101 72-73 1.2453910534624164 1101 74-75 1.0970571630796258 1101 76-77 1.0386009923297124 1101 78-79 1.4151799200405364 1101 80-81 2.374620743720534 1101 82-83 2.0785673445825026 1101 84-85 2.0003498098881316 1101 86-87 1.931956147816102 1101 88-89 1.8110736210808902 1101 90-91 1.7210868339797436 1101 92-93 1.34865586797941 1101 94-95 1.350308042196545 1101 96-97 1.5533975902329082 1101 98-99 1.534821987619356 1101 100-101 1.4064399286960754 1102 1 0.11638855744228849 1102 2 0.13048684292459356 1102 3 0.11438879904668653 1102 4 0.31021879828168153 1102 5 0.07318236516655219 1102 6 0.1717962910755233 1102 7 0.11943941227501398 1102 8 0.26758762643634526 1102 9 0.3919550590422034 1102 10-11 0.3323602536937642 1102 12-13 0.1969607529630153 1102 14-15 0.2342265078067456 1102 16-17 0.34063109251219004 1102 18-19 0.23441574555091194 1102 20-21 0.1676206903651689 1102 22-23 0.38953242580726055 1102 24-25 0.28598834840409637 1102 26-27 0.24086719431567616 1102 28-29 0.2873521831380046 1102 30-31 0.6329492425869034 1102 32-33 0.5895237692796798 1102 34-35 0.6281966833467223 1102 36-37 0.6723630028464385 1102 38-39 0.5289286662750854 1102 40-41 0.5546264055676104 1102 42-43 0.38413659780228926 1102 44-45 0.6485051595114513 1102 46-47 1.076130755506064 1102 48-49 0.9964998772188949 1102 50-51 0.9046943691041989 1102 52-53 0.9692248851490888 1102 54-55 1.0100816763167444 1102 56-57 0.9416211099001472 1102 58-59 0.8299728755747253 1102 60-61 0.7026640119781753 1102 62-63 1.0708640116110182 1102 64-65 1.2919770622001607 1102 66-67 1.1171895956758 1102 68-69 1.1593489698898978 1102 70-71 1.121507242493454 1102 72-73 1.3962833013481095 1102 74-75 1.1371731656436772 1102 76-77 0.8613558981422464 1102 78-79 1.0071228079047252 1102 80-81 1.3499614107869675 1102 82-83 1.6110201474608559 1102 84-85 1.403105129257046 1102 86-87 1.809629272854874 1102 88-89 1.9430125801594862 1102 90-91 2.0474996459563357 1102 92-93 1.5267994133982405 1102 94-95 1.607244416547779 1102 96-97 1.4047468179163722 1102 98-99 1.5506694680271202 1102 100-101 1.7350869790052084 1103 1 0.18222866093947587 1103 2 0.10127218193197152 1103 3 0.13194338674266604 1103 4 0.07403263645500857 1103 5 0.18300877442139551 1103 6 -0.05366992466287002 1103 7 0.07321094472396794 1103 8 -0.07919867765736655 1103 9 -0.038666880864532516 1103 10-11 0.025272484915142 1103 12-13 0.033568932069727 1103 14-15 0.09480065098063051 1103 16-17 -0.004197999849075984 1103 18-19 0.04731483163078565 1103 20-21 0.12063720914899534 1103 22-23 0.22722008726163523 1103 24-25 0.3683014773221558 1103 26-27 0.2735147755680387 1103 28-29 0.5046172449326534 1103 30-31 0.49145281798219287 1103 32-33 0.3286509485236593 1103 34-35 0.47284690194710066 1103 36-37 0.10455112812463341 1103 38-39 0.09358841094319104 1103 40-41 0.41484280121972716 1103 42-43 0.3012722881094092 1103 44-45 0.5526839417555252 1103 46-47 0.9778073850252227 1103 48-49 0.7161682951844739 1103 50-51 0.829163883607535 1103 52-53 1.1196098797204712 1103 54-55 1.2034192527020835 1103 56-57 1.0153274990213035 1103 58-59 1.0070932576158071 1103 60-61 0.8840544850784688 1103 62-63 0.7239716312172426 1103 64-65 0.8135032713659882 1103 66-67 1.0564983525863596 1103 68-69 1.2361045326558688 1103 70-71 1.0157450114800675 1103 72-73 1.2112591949889442 1103 74-75 1.5103298299392272 1103 76-77 1.551784695985777 1103 78-79 1.5806825621404954 1103 80-81 1.6264659263094643 1103 82-83 1.871823290647221 1103 84-85 1.6913237961899412 1103 86-87 1.5659293731073767 1103 88-89 1.9579797381050135 1103 90-91 2.380216280800113 1103 92-93 2.115624795168923 1103 94-95 2.058748828073906 1103 96-97 1.6578511096386777 1103 98-99 1.5508171428762232 1103 100-101 1.4484230578427795 1104 1 0.022904138178830635 1104 2 -0.03586322603864289 1104 3 -0.04076609982099555 1104 4 0.07891526918084679 1104 5 0.00821651642316823 1104 6 -0.04399670358085217 1104 7 -0.013775265745117338 1104 8 0.0490319726896189 1104 9 0.06542031726204556 1104 10-11 0.01676052128765093 1104 12-13 0.07550905613697267 1104 14-15 0.10483990897457574 1104 16-17 0.19534991245618727 1104 18-19 -0.06665215226787069 1104 20-21 0.09561398399990395 1104 22-23 -0.012694632559586694 1104 24-25 -0.01744577311986717 1104 26-27 0.14405436963095752 1104 28-29 -0.05234751705773277 1104 30-31 0.11911942899412509 1104 32-33 0.22666150579880906 1104 34-35 0.11852531176288039 1104 36-37 0.15189083178456997 1104 38-39 0.6130782710785567 1104 40-41 0.6116903885869576 1104 42-43 0.6638093734281156 1104 44-45 0.5237188629612675 1104 46-47 0.6241386676956253 1104 48-49 0.49558931469359635 1104 50-51 0.6350894004473773 1104 52-53 0.7389475455608832 1104 54-55 0.6494844629271412 1104 56-57 0.6909727728640007 1104 58-59 0.4588288291257143 1104 60-61 0.6047362127726963 1104 62-63 0.6660725118463908 1104 64-65 0.9051366522199018 1104 66-67 0.8001858212805679 1104 68-69 0.5433379357716781 1104 70-71 0.3657940304583356 1104 72-73 0.7866606582803612 1104 74-75 0.8674876710492363 1104 76-77 0.6745419171876605 1104 78-79 0.951547522347358 1104 80-81 1.1546839473127086 1104 82-83 1.3124568127785388 1104 84-85 1.2709207702847998 1104 86-87 1.035915416612255 1104 88-89 1.1782226496182666 1104 90-91 1.2353480754940769 1104 92-93 1.4059423696499458 1104 94-95 2.0633371328734853 1104 96-97 2.1776203137331542 1104 98-99 2.239124939248388 1104 100-101 1.8043661273298586 1105 1 -0.15582234517862403 1105 2 -0.08997207238884286 1105 3 -0.0850716864343859 1105 4 -0.06967362678688716 1105 5 0.05303051396761305 1105 6 -0.05617114126992817 1105 7 -0.0329324427776001 1105 8 0.11462542115025087 1105 9 -0.09827956417905881 1105 10-11 0.026461465961574504 1105 12-13 -0.016298144311946317 1105 14-15 0.19505226019581556 1105 16-17 -0.07036336065541349 1105 18-19 -0.15870933900446715 1105 20-21 0.10279080552446374 1105 22-23 0.023655694215740652 1105 24-25 -0.022067882455644394 1105 26-27 0.1705919435193124 1105 28-29 0.2562009591861809 1105 30-31 -0.011935618619297372 1105 32-33 0.0067558466155617225 1105 34-35 0.3568856548664243 1105 36-37 0.33964530094871037 1105 38-39 0.22345829242760118 1105 40-41 0.38817838713556085 1105 42-43 0.4443994676423557 1105 44-45 0.3627561731510056 1105 46-47 0.5440396857832397 1105 48-49 0.5119217521548336 1105 50-51 0.7339827100970595 1105 52-53 0.957137054153236 1105 54-55 0.7178931578088594 1105 56-57 0.39591406300420573 1105 58-59 0.45033667890611184 1105 60-61 0.43727493427742914 1105 62-63 0.3572023232913395 1105 64-65 0.2132442243783892 1105 66-67 0.4289139923091625 1105 68-69 0.4988024039654029 1105 70-71 0.6116455238363336 1105 72-73 0.43188265032299356 1105 74-75 0.9119514768320407 1105 76-77 0.8765645910494371 1105 78-79 0.8250048854455869 1105 80-81 0.5356050360293594 1105 82-83 1.0383199510874093 1105 84-85 0.9456031716720688 1105 86-87 0.8558293031923156 1105 88-89 0.9676371543078837 1105 90-91 1.5310713058654066 1105 92-93 1.142854479956128 1105 94-95 1.342822857285224 1105 96-97 1.2057864902719935 1105 98-99 1.270583129305475 1105 100-101 1.010440266723057 1106 1 -0.03069351233511952 1106 2 -0.09402884596817529 1106 3 0.0012532252155033063 1106 4 -0.0948366733834689 1106 5 -0.12242441206905852 1106 6 0.0021303690228862138 1106 7 0.07027408716155747 1106 8 0.013312335558488542 1106 9 0.012728126477611568 1106 10-11 0.07593327786668169 1106 12-13 0.1657275223780985 1106 14-15 0.3473704013477388 1106 16-17 0.04132329236825427 1106 18-19 -0.03711982328844954 1106 20-21 0.02739198154253586 1106 22-23 0.19124358212213366 1106 24-25 0.18695052284345337 1106 26-27 0.4563341588279499 1106 28-29 0.29610035800166656 1106 30-31 0.1478404831804312 1106 32-33 0.41455403222410325 1106 34-35 0.28929330429657796 1106 36-37 0.5362691897986451 1106 38-39 0.6655414212221942 1106 40-41 0.5440645339656882 1106 42-43 0.8570725817951583 1106 44-45 0.5636971576145555 1106 46-47 0.7971906449371247 1106 48-49 0.5069171938628827 1106 50-51 0.2373870847822772 1106 52-53 0.740021039779208 1106 54-55 0.6336688855948864 1106 56-57 0.34131868844860946 1106 58-59 0.6796336394072853 1106 60-61 0.8789149843442523 1106 62-63 0.5800656106592363 1106 64-65 0.7775267662264831 1106 66-67 0.9375556213088245 1106 68-69 0.9952822125383953 1106 70-71 0.863028418234844 1106 72-73 1.2391593418318188 1106 74-75 1.2753812249465675 1106 76-77 0.8212413773367331 1106 78-79 0.8310496693357976 1106 80-81 1.0904894211699663 1106 82-83 1.2895912788997883 1106 84-85 1.0877995671150522 1106 86-87 1.201024820978155 1106 88-89 1.30526314485979 1106 90-91 1.3288212734579687 1106 92-93 1.1956541637445461 1106 94-95 1.2870895035511438 1106 96-97 1.0051345163934506 1106 98-99 0.9840036333314366 1106 100-101 0.7604641738486553 1107 1 -0.20503703829175635 1107 2 -0.229882262224546 1107 3 -0.15203346390948624 1107 4 -0.15459956532438213 1107 5 -0.37054784646483796 1107 6 -0.22944516868118114 1107 7 -0.4421794845755116 1107 8 -0.23595345005090707 1107 9 -0.09319741554536165 1107 10-11 0.030960486988220737 1107 12-13 -0.11739310472736975 1107 14-15 -0.02707785813226593 1107 16-17 -0.11960678450831352 1107 18-19 -0.21027888867671862 1107 20-21 -0.04857869307092244 1107 22-23 -0.20010888397444404 1107 24-25 -0.1363683421474775 1107 26-27 -0.2781588013517151 1107 28-29 -0.17491868838340707 1107 30-31 0.020547142936841567 1107 32-33 0.15702293608521245 1107 34-35 -0.10257527132749544 1107 36-37 0.03411729379794792 1107 38-39 -0.30299101377823945 1107 40-41 -0.22031527081966118 1107 42-43 -0.33437490028136807 1107 44-45 -0.15808957469950968 1107 46-47 -0.13083475024297542 1107 48-49 -0.11785606345765842 1107 50-51 -0.09859050008426351 1107 52-53 -0.10652707285791507 1107 54-55 -0.2795221397139116 1107 56-57 -0.16187622387754885 1107 58-59 0.010948962893507996 1107 60-61 0.002763995314282397 1107 62-63 -0.5028757817567602 1107 64-65 -0.5129262872415978 1107 66-67 0.013213175079233253 1107 68-69 -0.010737265737500223 1107 70-71 0.046146885886219735 1107 72-73 0.004869370336614054 1107 74-75 -0.050114369767090494 1107 76-77 -0.37283631982142396 1107 78-79 -0.23370372100712444 1107 80-81 0.01562546677763521 1107 82-83 -0.3607574729357452 1107 84-85 -0.33061841681860926 1107 86-87 -0.37845604625860574 1107 88-89 -0.23645750815913047 1107 90-91 0.24400439516480432 1107 92-93 0.42734307278834294 1107 94-95 0.4272348348114008 1107 96-97 0.6297447349302026 1107 98-99 1.426352687490546 1107 100-101 1.316900426763917 1108 1 -0.17352443324974232 1108 2 -0.0961462678267857 1108 3 0.051678020684349235 1108 4 -0.04237967736920467 1108 5 -0.0800366419830496 1108 6 -0.020551611258213143 1108 7 -0.07885665544385745 1108 8 -0.11392964052709686 1108 9 -0.12308186932687448 1108 10-11 -0.1498605914431579 1108 12-13 -0.08456747447527135 1108 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-1.964271997570819 2316 52-53 -1.928101947185457 2316 54-55 -2.1827907260089994 2316 56-57 -1.8152263870312666 2316 58-59 -1.7264804841476753 2316 60-61 -1.570796283369905 2316 62-63 -1.9110918942887274 2316 64-65 -1.7121479999146736 2316 66-67 -2.1085314673054576 2316 68-69 -1.8942311805448035 2316 70-71 -1.7422372308789278 2316 72-73 -2.0636000714130986 2316 74-75 -2.5645259139949736 2316 76-77 -2.4960295026417825 2316 78-79 -2.5624320039244886 2316 80-81 -2.83410555006688 2316 82-83 -3.1471705164140076 2316 84-85 -3.576876905221468 2316 86-87 -3.707007946778937 2316 88-89 -3.489610343506161 2316 90-91 -3.6208888934849917 2316 92-93 -4.12716680462589 2316 94-95 -4.302590326137533 2316 96-97 -4.159349320983015 2316 98-99 -4.520999371773943 2316 100-101 -4.16716232127383 >>END_MODULE >>Per sequence quality scores pass #Quality Count 3 5.0 4 14.0 5 86.0 6 250.0 7 350.0 8 454.0 9 801.0 10 1286.0 11 1734.0 12 2583.0 13 3737.0 14 4467.0 15 5969.0 16 7409.0 17 10147.0 18 9635.0 19 7408.0 20 7362.0 21 8111.0 22 8968.0 23 9919.0 24 11489.0 25 13221.0 26 15191.0 27 18104.0 28 21515.0 29 25764.0 30 30004.0 31 35130.0 32 42533.0 33 52611.0 34 69401.0 35 90093.0 36 91127.0 37 68669.0 38 20034.0 39 462.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.421534293167305 24.020061394785767 17.173966304927436 15.384438007119488 2 14.774805579540345 29.66741997261663 35.29293448824282 20.2648399596002 3 26.31245483396859 27.84554977206868 31.97604171006676 13.865953683895965 4 11.756744913748145 20.643695863617623 41.92858774529735 25.67097147733689 5 8.936137199208664 43.76665675342905 35.6941205880705 11.603085459291792 6 22.858501558093394 43.38036586820067 16.479728982261154 17.281403591444782 7 21.890601586396244 38.27421581712624 23.01826180279092 16.816920793686595 8 22.830917055411806 30.75600214354573 20.583211094716848 25.829869706325614 9 20.917529520446294 24.37823525270709 25.66148930454009 29.042745922306523 10-11 23.263936279798806 27.319361016488923 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17.73632995176925 36-37 20.818064458657478 27.6418775094415 34.44636575040248 17.093692281498544 38-39 18.517842658721747 30.356407745576785 34.82470578346266 16.301043812238806 40-41 18.404118219212133 31.133688395167443 32.28392782484638 18.178265560774047 42-43 16.750282310550226 32.62353421201594 30.102421980282802 20.523761497151032 44-45 20.22268835124418 27.92167116832366 30.408166197344983 21.44747428308718 46-47 21.977297314382344 31.87556480149876 29.77030088744772 16.376836996671173 48-49 20.926395246263873 30.06082966257783 31.310251392875717 17.702523698282572 50-51 16.04750879799207 31.49002319552267 34.5689432855463 17.89352472093897 52-53 18.99049340342479 34.21871924579372 29.00603842004014 17.784748930741348 54-55 16.87201796440737 29.98011617098369 30.836816116245693 22.31104974836325 56-57 20.531130978976876 29.834004508342154 30.10489294483243 19.529971567848538 58-59 24.064404298313104 28.641005803541603 28.89537318589938 18.39921671224591 60-61 18.738295165485795 31.482637514519922 33.08746146878028 16.691605851213996 62-63 18.436504641236244 29.990029351634885 32.807527695846375 18.765938311282493 64-65 18.001969712969288 34.22351141923494 30.22166095220034 17.55285791559543 66-67 19.42824344622829 29.732533272608023 28.843419936339 21.995803344824687 68-69 17.55472832310148 30.879029465726283 30.263178689393687 21.303063521778544 70-71 21.388908443874875 31.209135067804716 29.145397626296077 18.25655886202433 72-73 20.504623995931286 27.00996921167227 32.941140130710316 19.544266661686134 74-75 16.865696515876174 35.10336286694931 29.24409842495363 18.78684219222088 76-77 19.505122558165787 29.243842971968114 29.37096323075275 21.88007123911335 78-79 19.078634509649547 29.884145088737334 31.13801877182875 19.899201629784365 80-81 20.638380100080024 29.574465945351076 30.34676018579312 19.44039376877578 82-83 19.063764738672752 29.226858110777638 32.23177303701065 19.477604113538963 84-85 17.290454756387177 30.355667681450715 31.866853053618815 20.487024508543293 86-87 18.213150113888826 31.013506272695956 29.555283369929754 21.218060243485464 88-89 18.092095300044566 30.50507177502751 29.01346656147643 22.38936636345149 90-91 19.647865861346734 26.653227055694888 30.643970151382955 23.054936931575423 92-93 20.254783109664203 30.94945283552884 30.457457369731465 18.33830668507549 94-95 19.296437145406248 29.542858702695092 30.574476002201013 20.586228149697646 96-97 15.308923285134371 29.666462415512935 32.76384855148099 22.260765747871705 98-99 18.372859148069875 30.984220802450423 28.38186721222683 22.26105283725287 100-101 17.814344803410133 27.940012326824636 30.119475377239624 24.126167492525603 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 15570.0 1 9012.0 2 2169.5 3 1668.0 4 1203.5 5 1177.0 6 2135.5 7 3894.0 8 5082.0 9 4958.0 10 3591.5 11 2181.5 12 1815.5 13 1683.0 14 1521.5 15 1352.5 16 1229.0 17 1208.5 18 1230.5 19 1349.5 20 1603.5 21 1877.5 22 2134.0 23 2323.0 24 2507.5 25 3278.0 26 5194.0 27 6763.0 28 6861.5 29 7289.0 30 8865.0 31 10581.0 32 13303.5 33 17129.0 34 20724.5 35 22656.5 36 24967.0 37 27558.0 38 26045.5 39 22757.0 40 19964.0 41 17196.0 42 14942.0 43 14233.0 44 14617.5 45 15117.5 46 18864.5 47 41843.0 48 86258.5 49 83568.0 50 40619.0 51 18433.0 52 8472.5 53 3875.5 54 1994.5 55 1277.5 56 938.0 57 688.5 58 576.0 59 505.5 60 409.5 61 318.5 62 222.0 63 155.0 64 87.5 65 45.5 66 31.0 67 23.5 68 28.0 69 26.5 70 12.5 71 7.5 72 5.0 73 3.0 74 3.0 75 3.5 76 3.0 77 1.0 78 2.0 79 2.0 80 0.5 81 1.0 82 1.0 83 0.5 84 0.0 85 0.5 86 0.5 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.31219335587025515 2 0.0 3 0.0 4 0.0 5 5.746771391997333E-4 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0011493542783994667 16-17 0.0017958660599991668 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0020113699871990667 36-37 0.006680621743196901 38-39 0.012140054565594367 40-41 0.0020113699871990667 42-43 1.4366928479993333E-4 44-45 4.310078543998E-4 46-47 2.8733856959986667E-4 48-49 1.4366928479993333E-4 50-51 5.028424967997667E-4 52-53 0.0 54-55 0.0 56-57 0.0 58-59 3.5917321199983334E-4 60-61 0.0023705431991989 62-63 0.0 64-65 3.5917321199983334E-4 66-67 0.0017958660599991668 68-69 0.0020113699871990667 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.09173283834475744 78-79 0.0 80-81 0.0 82-83 0.0 84-85 0.0 86-87 0.12175971886794351 88-89 0.38733239182062024 90-91 0.2980419313174617 92-93 0.0 94-95 0.0 96-97 6.465117815997E-4 98-99 0.0 100-101 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 696043.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 17.469973073922407 #Duplication Level Percentage of deduplicated Percentage of total 1 74.70131724472535 13.050300008518873 2 9.80645739513673 3.4263709328721195 3 3.8729016955768407 2.029784650190275 4 2.132399234915343 1.4901182886729516 5 1.3691583706837145 1.1959579934889983 6 0.981865398954723 1.0291897249173054 7 0.7295191679856262 0.8921276155133417 8 0.5885467995101592 0.8225517392148554 9 0.4698456627885141 0.7387371970033112 >10 4.153593863044227 15.496607567173678 >50 0.6767414988566425 8.332596261535334 >100 0.4525348147043809 14.668157320613668 >500 0.024728678399146494 2.960992056022562 >1k 0.025552967679118046 9.87717367583832 >5k 0.013188628479544797 17.4144407317802 >10k+ 0.0016485785599430996 6.574894236644205 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 35112 5.044515927895259 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 10546 1.515136277500097 No Hit ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC 9796 1.407384313900147 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 9654 1.3869832754585565 No Hit TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC 9401 1.3506349464041734 No Hit GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA 9034 1.2979083188825977 No Hit GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG 8889 1.2770762725866074 No Hit GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT 8495 1.2204705743754338 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 8471 1.2170225115402353 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 8445 1.213287110135437 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 7139 1.0256550241867242 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6368 0.9148860056059755 No Hit GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA 6327 0.9089955649291782 No Hit GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA 6201 0.8908932350443867 No Hit TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT 6016 0.864314417356399 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5853 0.8408963239340098 No Hit GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 5511 0.7917614285324327 No Hit AAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGC 5331 0.7659009572684446 No Hit ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC 4808 0.6907619213180795 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4158 0.5973768861981229 No Hit TGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGAT 4029 0.5788435484589314 No Hit GATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATA 3890 0.5588735178717408 No Hit GCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAG 3332 0.47870605695337787 No Hit GTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTG 3227 0.4636207820493849 No Hit CAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGT 2908 0.41779028019820613 No Hit CCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCAC 2777 0.3989696038894148 No Hit CTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTG 2671 0.38374065970062193 No Hit TACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACC 2475 0.355581479879835 No Hit ATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATAC 2386 0.3427949135326409 No Hit ACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTG 2205 0.316790772983853 No Hit TGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCAC 2033 0.29207965599826446 No Hit CTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCA 1974 0.2836031681950684 No Hit GCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCT 1971 0.2831721603406686 No Hit GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT 1909 0.27426466468307276 No Hit GGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGT 1893 0.27196595612627383 No Hit AACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACG 1873 0.2690925704302751 No Hit AGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAG 1839 0.26420781474707744 No Hit TATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGG 1725 0.247829516279885 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1615 0.23202589495189235 No Hit GTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTG 1545 0.22196904501589698 No Hit AGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTA 1534 0.22038868288309774 No Hit ACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCA 1422 0.20429772298550522 No Hit ATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCA 1385 0.19898195944790764 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1296 0.18619539310071362 No Hit ATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTG 1217 0.17484551960151887 No Hit GTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCA 1206 0.1732651574687196 No Hit CAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAG 1129 0.16220262253912474 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1092 0.1568868590015272 No Hit CAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGA 1066 0.15315145759672893 No Hit CACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACT 968 0.13907186768633548 No Hit ACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGT 953 0.13691682841433647 No Hit TTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGA 944 0.13562380485113706 No Hit TACTAACAGTGTTGCATCTATAAAGTTATAGATTAACCCAATTTTAAGTT 896 0.12872767918074027 No Hit GCTTTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC 867 0.1245612699215422 No Hit CAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAAC 832 0.11953284495354453 No Hit GAGTAAGCAGTGGTATCAACGCAGAGCAAGCAGTGGTATCAACGCAGAGT 821 0.11795248282074526 No Hit GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 795 0.114217081415947 No Hit GTTATAGATTAACCCAATTTTAAGTTTAGGAAGTTGGTGTAAATTATGGA 776 0.11148736500474828 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGC 738 0.1060279321823508 No Hit GGATATGAGATTGGCTTGAAACCAATTTTAGGGGGTTCGATTCCTTCCTT 722 0.10372922362555186 No Hit TGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGT 715 0.10272353863195233 No Hit GATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTT 713 0.10243620006235246 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 1.4366928479993333E-4 0.0 14-15 0.0 0.0 0.0 1.4366928479993333E-4 0.0 16-17 0.0 0.0 0.0 2.155039271999E-4 0.0 18-19 0.0 0.0 0.0 3.5917321199983334E-4 0.0 20-21 0.0 0.0 0.0 4.310078543998E-4 0.0 22-23 0.0 0.0 0.0 0.0010775196359995 0.0 24-25 0.0 0.0 0.0 0.0019395353447991001 0.0 26-27 0.0 0.0 0.0 0.003950905331998167 0.0 28-29 0.0 0.0 0.0 0.014726101691993166 0.0 30-31 0.0 0.0 0.0 0.038287864399182234 0.0 32-33 0.0 0.0 0.0 0.0801674609183628 0.0 34-35 0.0 0.0 0.0 0.1461116626415322 0.0 36-37 0.0 0.0 0.0 0.23303157994549187 0.0 38-39 0.0 0.0 0.0 0.3478233385006386 0.0 40-41 0.0 0.0 0.0 0.4705169077197817 0.0 42-43 0.0 0.0 0.0 0.6037701693717199 0.0 44-45 0.0 0.0 0.0 0.7729407522236413 0.0 46-47 0.0 0.0 0.0 0.9839909315947435 0.0 48-49 0.0 0.0 0.0 1.166163584721059 0.0 50-51 0.0 0.0 0.0 1.3700302998521643 0.0 52-53 0.0 0.0 0.0 1.5636246611200746 0.0 54-55 0.0 0.0 0.0 1.7921306585943686 0.0 56-57 0.0 0.0 0.0 2.1198402972230164 0.0 58-59 0.0 0.0 0.0 2.417810393898078 0.0 60-61 0.0 0.0 0.0 2.775977920904312 0.0 62-63 0.0 0.0 0.0 3.1306255504329474 0.0 64-65 0.0 0.0 0.0 3.454527953014397 0.0 66-67 0.0 0.0 0.0 3.8585978165142096 0.0 68-69 0.0 0.0 0.0 4.330695086366791 0.0 70-71 0.0 0.0 0.0 4.841654897757754 0.0 72-73 0.0 0.0 0.0 5.3203609547111315 0.0 74-75 0.0 0.0 0.0 5.898917164600463 0.0 76-77 0.0 0.0 0.0 6.39953278748583 0.0 78-79 0.0 0.0 0.0 7.135047691019089 0.0 80-81 0.0 0.0 0.0 7.9147408996283275 0.0 82-83 0.0 0.0 0.0 8.550549319510434 0.0 84-85 0.0 0.0 0.0 9.303017198650084 0.0 86-87 0.0 0.0 0.0 10.087379659015319 0.0 88-89 0.0 0.0 0.0 10.876195867209354 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GCGTCAG 25 5.223805E-5 76.14637 1 CGAGAGT 20 0.0020804007 71.19235 8 CGTCAGA 30 1.3060865E-4 63.282085 2 TACCTGG 150 0.0 50.62567 2 GACATAT 210 0.0 49.857746 1 ACCTGGG 180 0.0 44.82481 3 GGAACAT 45 9.660863E-4 42.188057 8 GTAAGCG 300 0.0 41.924385 94-95 CATATAG 260 0.0 40.159786 3 CATTCCG 145 0.0 39.278538 9 CGCCCCT 25 0.0016223721 37.969254 78-79 CTCCGAC 120 0.0 35.596172 94-95 GTCAGAT 60 0.0039655725 31.641043 3 TAAGAGT 60 0.0039655725 31.641043 4 CCTTTAG 190 1.2732926E-11 30.057781 1 ACATATA 365 0.0 29.907288 2 ATCTCGG 40 4.5154776E-4 29.663479 92-93 GACCCAC 80 4.513183E-4 29.663479 7 TAGGCAT 160 1.9190338E-9 29.663479 5 TTAGGCA 195 1.6370905E-11 29.207117 4 >>END_MODULE