##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938873_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2124458 Sequences flagged as poor quality 0 Sequence length 101 %GC 43 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.871700923247246 33.0 31.0 34.0 30.0 34.0 2 31.919278705439222 33.0 31.0 34.0 30.0 34.0 3 31.964310897179423 34.0 31.0 34.0 30.0 34.0 4 35.474495612527996 37.0 35.0 37.0 33.0 37.0 5 35.53975555176897 37.0 35.0 37.0 33.0 37.0 6 35.64997566438122 37.0 35.0 37.0 33.0 37.0 7 35.5961078072619 37.0 35.0 37.0 33.0 37.0 8 35.59974073387188 37.0 35.0 37.0 33.0 37.0 9 37.17525317045571 39.0 37.0 39.0 33.0 39.0 10-11 37.07059235814499 39.0 37.0 39.0 33.0 39.0 12-13 36.974362402080914 39.0 37.0 39.0 33.0 39.0 14-15 38.05848315193805 40.0 38.0 41.0 33.0 41.0 16-17 38.00598482059895 40.0 38.0 41.0 33.0 41.0 18-19 38.02936160658389 40.0 38.0 41.0 33.0 41.0 20-21 38.15044872621628 40.0 38.0 41.0 33.0 41.0 22-23 38.11855682720016 40.0 38.0 41.0 33.0 41.0 24-25 38.02210187257173 40.0 38.0 41.0 33.0 41.0 26-27 37.82234645260108 40.0 37.5 41.0 32.5 41.0 28-29 37.68325403467614 40.0 37.0 41.0 32.0 41.0 30-31 37.53239390941125 40.0 37.0 41.0 32.0 41.0 32-33 37.319522202839494 40.0 36.5 41.0 32.0 41.0 34-35 36.98605879711437 40.0 36.0 41.0 31.0 41.0 36-37 36.66013001904486 39.0 35.5 41.0 30.0 41.0 38-39 36.35742810636877 39.0 35.0 41.0 30.0 41.0 40-41 36.2086551016777 39.0 35.0 41.0 30.0 41.0 42-43 36.0501200776857 39.0 35.0 41.0 30.0 41.0 44-45 35.96242924077576 39.0 35.0 41.0 29.5 41.0 46-47 35.65727140757784 39.0 35.0 41.0 27.0 41.0 48-49 35.35777478302701 39.0 35.0 41.0 25.5 41.0 50-51 34.2147055860836 38.0 34.0 40.0 23.5 40.5 52-53 34.27131108263849 38.0 33.5 39.5 23.5 40.5 54-55 34.804934482112614 39.0 35.0 40.0 24.0 41.0 56-57 34.7733247727185 39.0 35.0 40.5 23.5 41.0 58-59 34.59990548177465 38.5 34.5 40.0 23.0 41.0 60-61 34.24210598656222 38.0 34.0 40.0 22.0 41.0 62-63 33.84089424220201 38.0 34.0 40.0 21.5 41.0 64-65 33.37626326338294 37.0 33.0 40.0 20.0 41.0 66-67 32.929305498155294 36.5 32.5 39.0 18.0 41.0 68-69 32.52116022063039 36.0 32.0 39.0 15.5 40.5 70-71 31.985522660367963 35.5 31.5 38.5 11.5 40.0 72-73 31.474658006889285 35.0 31.0 37.5 7.5 40.0 74-75 31.002898386317828 35.0 31.0 37.0 6.5 39.0 76-77 30.500631690530007 35.0 30.5 36.5 2.0 39.0 78-79 30.081802982219465 34.5 30.0 36.0 2.0 38.5 80-81 29.6621780708303 34.0 29.5 36.0 2.0 37.0 82-83 29.187464049654075 34.0 29.0 35.0 2.0 37.0 84-85 28.764447920363686 34.0 29.0 35.0 2.0 36.5 86-87 28.293778930908495 34.0 29.0 35.0 2.0 36.0 88-89 27.721098981481397 34.0 26.5 35.0 2.0 36.0 90-91 27.196621914860167 33.5 25.5 35.0 2.0 35.0 92-93 26.748385705907104 33.0 25.0 35.0 2.0 35.0 94-95 26.203197003659284 33.0 24.5 35.0 2.0 35.0 96-97 25.46804243717692 32.5 20.5 35.0 2.0 35.0 98-99 24.602580517007162 32.0 9.5 35.0 2.0 35.0 100-101 21.439241679524848 28.5 2.0 32.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.1836609870283894 1101 2 0.3174497665879237 1101 3 0.3718412799232702 1101 4 0.25033962359483297 1101 5 0.2150148811880186 1101 6 0.2748003101246539 1101 7 0.31121769454485815 1101 8 0.15671445882673396 1101 9 0.3556848426238446 1101 10-11 0.234296917011676 1101 12-13 0.2591048190395213 1101 14-15 0.42212296237538993 1101 16-17 0.39607998163925373 1101 18-19 0.5287994380669048 1101 20-21 0.4434529397915341 1101 22-23 0.5192672215946317 1101 24-25 0.5337154221614284 1101 26-27 0.5453245944137706 1101 28-29 0.566160712626484 1101 30-31 0.5644255032320729 1101 32-33 0.5128375445181703 1101 34-35 0.43791131509970427 1101 36-37 0.4251952536944117 1101 38-39 0.6080317415728942 1101 40-41 0.3895065077268143 1101 42-43 0.4702304779431401 1101 44-45 0.5893553689132247 1101 46-47 0.4755543414935559 1101 48-49 0.6499719109715656 1101 50-51 0.5448744919488178 1101 52-53 0.5317421722172568 1101 54-55 0.725468323772688 1101 56-57 0.6460024669771585 1101 58-59 0.6811543149137194 1101 60-61 0.6369862976620837 1101 62-63 0.6879415530885424 1101 64-65 0.49463817629339246 1101 66-67 0.52186396714837 1101 68-69 0.5086404042737627 1101 70-71 0.6508987105542303 1101 72-73 0.6445862806405991 1101 74-75 0.41023122503513676 1101 76-77 0.5794888675021426 1101 78-79 0.46434393540052454 1101 80-81 0.3688935336340151 1101 82-83 0.3816048892659474 1101 84-85 0.28532918822721953 1101 86-87 0.2651697198414986 1101 88-89 0.18990727503305393 1101 90-91 0.31601455699794556 1101 92-93 0.49678116565869246 1101 94-95 0.32552531564360976 1101 96-97 0.04793028712324343 1101 98-99 -0.09445829552945284 1101 100-101 0.5170276073977043 1102 1 0.2638254061536358 1102 2 0.22296082865645772 1102 3 0.35779968033740417 1102 4 0.17831053083868653 1102 5 0.27003962871363285 1102 6 0.3434990811897265 1102 7 0.24677949719033876 1102 8 0.2942623398031259 1102 9 0.23900195006552138 1102 10-11 0.40815588652402823 1102 12-13 0.3419626875266033 1102 14-15 0.47348374787397063 1102 16-17 0.5383883494799804 1102 18-19 0.5270422007597091 1102 20-21 0.3725701199213134 1102 22-23 0.475098982208479 1102 24-25 0.4409458935588546 1102 26-27 0.5215717865074083 1102 28-29 0.6108214455307959 1102 30-31 0.5562412802479386 1102 32-33 0.5879736400966991 1102 34-35 0.5854838535930114 1102 36-37 0.5974895315254614 1102 38-39 0.5834022003301769 1102 40-41 0.49069683621961957 1102 42-43 0.4352170958396897 1102 44-45 0.3038795367341933 1102 46-47 0.4420591871237818 1102 48-49 0.3493429934647807 1102 50-51 0.3920555412750417 1102 52-53 0.3658590677249478 1102 54-55 0.3698299845450066 1102 56-57 0.311234589669219 1102 58-59 0.3175433947670001 1102 60-61 0.5613731560034836 1102 62-63 0.5002369353506069 1102 64-65 0.3984029480580986 1102 66-67 0.353852035401232 1102 68-69 0.4061882511928161 1102 70-71 0.2636354516653796 1102 72-73 0.2852225545336502 1102 74-75 0.2577479824646254 1102 76-77 0.39498065311408226 1102 78-79 0.21164294476970014 1102 80-81 0.15544411827697502 1102 82-83 0.18481669140056667 1102 84-85 0.22463182519004476 1102 86-87 0.13955820807006347 1102 88-89 0.1547375940481892 1102 90-91 0.2079236024825022 1102 92-93 0.2876115420847789 1102 94-95 0.30794150903482986 1102 96-97 0.4719296215454385 1102 98-99 0.35195660952110686 1102 100-101 0.6837970542626515 1103 1 0.23277967592082405 1103 2 0.25604249557295233 1103 3 0.32393555788820905 1103 4 0.2639617613506644 1103 5 0.07803799297894187 1103 6 0.23402006656949936 1103 7 0.23940244045527237 1103 8 0.1336654033615119 1103 9 0.34118831418918205 1103 10-11 0.31371609897549746 1103 12-13 0.26546880625125624 1103 14-15 0.45023601410871095 1103 16-17 0.4352713338129135 1103 18-19 0.4481404374939828 1103 20-21 0.47735852463854656 1103 22-23 0.42475357150664195 1103 24-25 0.5048300164058261 1103 26-27 0.49280745018056393 1103 28-29 0.48589960619273853 1103 30-31 0.3583278121653848 1103 32-33 0.4002786628985149 1103 34-35 0.4405288726790104 1103 36-37 0.3535539257239435 1103 38-39 0.545108933279991 1103 40-41 0.5131992240915153 1103 42-43 0.596115517782593 1103 44-45 0.6596393408340973 1103 46-47 0.6339249967275649 1103 48-49 0.6084222426761343 1103 50-51 0.6180025525908164 1103 52-53 0.610413288788763 1103 54-55 0.5740619144548376 1103 56-57 0.44164562379602046 1103 58-59 0.5847629950597977 1103 60-61 0.6653805604679945 1103 62-63 0.6603810371736287 1103 64-65 0.6268929398551677 1103 66-67 0.7212333415538197 1103 68-69 0.5076982739485416 1103 70-71 0.5006706049233429 1103 72-73 0.5400485054742212 1103 74-75 0.39315766075558045 1103 76-77 0.3685390648991316 1103 78-79 0.3279558328746752 1103 80-81 0.38971108647765007 1103 82-83 0.3847697343667349 1103 84-85 0.39561909682629803 1103 86-87 0.4356390420813163 1103 88-89 0.6511531181988026 1103 90-91 0.6790314440646021 1103 92-93 0.42643445828151627 1103 94-95 0.3810984333297114 1103 96-97 0.3376342026212704 1103 98-99 0.49694421296119273 1103 100-101 0.9428275515948776 1104 1 0.16574605901966777 1104 2 0.28984364457440037 1104 3 0.18450992338619088 1104 4 0.1979220686171601 1104 5 0.1420545815636416 1104 6 0.15108076524796132 1104 7 0.17640537272475143 1104 8 0.10663699272980409 1104 9 0.20717299819446566 1104 10-11 0.33395583367791914 1104 12-13 0.3000409950649683 1104 14-15 0.35821966893400514 1104 16-17 0.31366026870339425 1104 18-19 0.34092968186289596 1104 20-21 0.3688882188474949 1104 22-23 0.3362241711766387 1104 24-25 0.29704420464917547 1104 26-27 0.28150100737381933 1104 28-29 0.341151430499977 1104 30-31 0.3743683621464129 1104 32-33 0.4034844064086087 1104 34-35 0.49303673218989985 1104 36-37 0.5467670125853346 1104 38-39 0.6282650820025353 1104 40-41 0.48405317060554154 1104 42-43 0.4461415129081203 1104 44-45 0.6510492377205068 1104 46-47 0.704089257426439 1104 48-49 0.5482533673293446 1104 50-51 0.48540906008955886 1104 52-53 0.39005998690071664 1104 54-55 0.600177296107475 1104 56-57 0.6621479177179381 1104 58-59 0.5629886665562722 1104 60-61 0.6401853314005947 1104 62-63 0.588901444856404 1104 64-65 0.5017983105954045 1104 66-67 0.5086218299511529 1104 68-69 0.5008076942294721 1104 70-71 0.5357680087673344 1104 72-73 0.43861813365337454 1104 74-75 0.3624764097730697 1104 76-77 0.44895407411997823 1104 78-79 0.41237044510540244 1104 80-81 0.5634063376403091 1104 82-83 0.6806041634072884 1104 84-85 0.4984363985443423 1104 86-87 0.528663679375061 1104 88-89 0.7205645706876105 1104 90-91 0.7489053272582318 1104 92-93 0.6894683031380673 1104 94-95 0.7081511608136211 1104 96-97 0.7101962691528492 1104 98-99 0.8160494107547613 1104 100-101 1.015242871839611 1105 1 0.09701584495303806 1105 2 0.17754229622577 1105 3 0.15243972051765198 1105 4 0.23460602541647546 1105 5 0.23917653158071772 1105 6 0.20698740557446627 1105 7 0.19255668075322063 1105 8 0.13880016087370706 1105 9 0.2170273173822892 1105 10-11 0.33500767942598486 1105 12-13 0.2665058848271684 1105 14-15 0.38967292042973156 1105 16-17 0.33718435692181004 1105 18-19 0.38885292916813086 1105 20-21 0.4227098311611641 1105 22-23 0.3489980930083334 1105 24-25 0.40140159979032575 1105 26-27 0.3975273052782313 1105 28-29 0.35104378093680566 1105 30-31 0.37324714458301855 1105 32-33 0.4303404546096985 1105 34-35 0.42388817438416737 1105 36-37 0.4632539111721883 1105 38-39 0.4437751409157542 1105 40-41 0.42367187212010293 1105 42-43 0.44719406293857134 1105 44-45 0.5470531394449409 1105 46-47 0.3369538908298466 1105 48-49 0.42006340828336164 1105 50-51 0.3912179812345826 1105 52-53 0.2896835479637687 1105 54-55 0.5380462080263939 1105 56-57 0.4783122549706107 1105 58-59 0.23002296863003124 1105 60-61 0.43479052830033993 1105 62-63 0.518207691489053 1105 64-65 0.5249456077356669 1105 66-67 0.4898727763306141 1105 68-69 0.35858279573417207 1105 70-71 0.2476379841526608 1105 72-73 0.3800397980839385 1105 74-75 0.31655262017038766 1105 76-77 0.44215105838278745 1105 78-79 0.4346592220133907 1105 80-81 0.5757376179904767 1105 82-83 0.6709389440409304 1105 84-85 0.6017711756565731 1105 86-87 0.5143818889483676 1105 88-89 0.6833126101415097 1105 90-91 0.8571380362452281 1105 92-93 0.8382729598532244 1105 94-95 0.5529745091833824 1105 96-97 0.5851572695155589 1105 98-99 0.6770416742127132 1105 100-101 0.7112862133778357 1106 1 0.057642129843298306 1106 2 0.06060901126477347 1106 3 0.19847489998587875 1106 4 0.09656369049278624 1106 5 0.08237736577540034 1106 6 0.13901012232019383 1106 7 0.16127072949520027 1106 8 0.07739672415590348 1106 9 0.1003612211441478 1106 10-11 0.07528940266774953 1106 12-13 0.12228147368053754 1106 14-15 0.12469175597974669 1106 16-17 0.1410617878029896 1106 18-19 0.31034405215937255 1106 20-21 0.2522347554653024 1106 22-23 0.2311755099710524 1106 24-25 0.2654306049401569 1106 26-27 0.295512323543349 1106 28-29 0.22300893003541233 1106 30-31 0.21844059971424912 1106 32-33 0.4109738356308483 1106 34-35 0.17750606028477733 1106 36-37 0.28548111513688923 1106 38-39 0.37748883599204675 1106 40-41 0.376204574787657 1106 42-43 0.315868523940658 1106 44-45 0.3865893125996607 1106 46-47 0.4785199909469 1106 48-49 0.34859898134727274 1106 50-51 0.14744957065381925 1106 52-53 0.3000798360712409 1106 54-55 0.3273186150294265 1106 56-57 0.306862202833436 1106 58-59 0.33103525109711285 1106 60-61 0.5052045681436326 1106 62-63 0.5057675579631464 1106 64-65 0.3565960628235487 1106 66-67 0.22530985007789894 1106 68-69 0.19773130566077413 1106 70-71 0.2621418140098086 1106 72-73 0.1913874468916319 1106 74-75 0.20342363715318612 1106 76-77 0.27428626363438013 1106 78-79 0.4671069080130188 1106 80-81 0.43307618101256296 1106 82-83 0.5919304857267065 1106 84-85 0.5845814260290645 1106 86-87 0.5564674212836813 1106 88-89 0.7676959883105248 1106 90-91 0.7983055209064034 1106 92-93 0.761289979158807 1106 94-95 1.0275855339696456 1106 96-97 1.0592779539385226 1106 98-99 1.0839118013270088 1106 100-101 1.2812058057724265 1107 1 0.12733353173188533 1107 2 0.15688371760343145 1107 3 0.23293296305442723 1107 4 0.2846068686237686 1107 5 0.2779121253841552 1107 6 0.16852636006488808 1107 7 0.19496622990714485 1107 8 0.22182707267759838 1107 9 0.1877633024339076 1107 10-11 0.25438289655185997 1107 12-13 0.2720582361950221 1107 14-15 0.3870657234868631 1107 16-17 0.28109150968061414 1107 18-19 0.3770156479382649 1107 20-21 0.36050103279901435 1107 22-23 0.29627402753497734 1107 24-25 0.2098616671794602 1107 26-27 0.38485794709389154 1107 28-29 0.4426103588980652 1107 30-31 0.496124032945751 1107 32-33 0.45106480340070476 1107 34-35 0.3622939054954841 1107 36-37 0.3403928751065308 1107 38-39 0.45269593676852793 1107 40-41 0.37264568884731375 1107 42-43 0.5121061838747423 1107 44-45 0.3800373849599197 1107 46-47 0.5112583291357353 1107 48-49 0.5352843117293844 1107 50-51 0.4757093754609727 1107 52-53 0.4119759250325714 1107 54-55 0.45496600552173305 1107 56-57 0.513567089078002 1107 58-59 0.6503570810869022 1107 60-61 0.5183098731620035 1107 62-63 0.5157350704297841 1107 64-65 0.45142872503929254 1107 66-67 0.43244631243843656 1107 68-69 0.5758472963699504 1107 70-71 0.46177654672032986 1107 72-73 0.5260846499661476 1107 74-75 0.4577301422877156 1107 76-77 0.5023152477064343 1107 78-79 0.6279341379456262 1107 80-81 0.5821365494782 1107 82-83 0.5218217820743014 1107 84-85 0.5557195781160402 1107 86-87 0.7534200255277135 1107 88-89 0.7924917647081564 1107 90-91 0.9261525292921391 1107 92-93 0.8644643274342627 1107 94-95 0.8297003015452731 1107 96-97 0.6822279673876395 1107 98-99 0.6684392734329165 1107 100-101 0.8448762190104624 1108 1 0.21313602006601684 1108 2 0.2096188462879276 1108 3 0.20775703914432242 1108 4 0.1966597730817341 1108 5 0.27405418228570966 1108 6 0.28993038779803015 1108 7 0.31220268385246186 1108 8 0.3979850488355723 1108 9 0.33411379561984234 1108 10-11 0.29488312059638844 1108 12-13 0.3909309639285041 1108 14-15 0.5233344432998877 1108 16-17 0.4010080890908654 1108 18-19 0.38105651273862406 1108 20-21 0.3273327667193584 1108 22-23 0.4976367251002074 1108 24-25 0.4920520278508249 1108 26-27 0.5651172627962424 1108 28-29 0.5919879297946196 1108 30-31 0.5871412872541413 1108 32-33 0.6069246909061619 1108 34-35 0.5964928915932006 1108 36-37 0.5998031484408486 1108 38-39 0.7028209755011616 1108 40-41 0.7163821756217743 1108 42-43 0.7698509251637873 1108 44-45 0.7280546772810084 1108 46-47 0.9675479904886899 1108 48-49 1.0725684312919839 1108 50-51 0.9121331409986624 1108 52-53 0.9313759925339085 1108 54-55 0.9667778173335435 1108 56-57 0.9581041462732927 1108 58-59 0.9598281171402121 1108 60-61 0.9548218742309658 1108 62-63 1.1125842786713989 1108 64-65 1.125033399909789 1108 66-67 1.0470649587026486 1108 68-69 1.0681215038340284 1108 70-71 1.1315561076391276 1108 72-73 1.086308395000021 1108 74-75 1.094464344844706 1108 76-77 1.2277007022058157 1108 78-79 1.251778695967399 1108 80-81 1.272231823117778 1108 82-83 1.1890423973961823 1108 84-85 1.2334706318164628 1108 86-87 1.3346683624722537 1108 88-89 1.4977888915242694 1108 90-91 1.4394128552106942 1108 92-93 1.476527368611226 1108 94-95 1.4786828859708976 1108 96-97 1.502903072872872 1108 98-99 1.849015937553883 1108 100-101 1.73851416074967 1109 1 0.016034206905811033 1109 2 0.07476393497316636 1109 3 0.09356134876250266 1109 4 0.10980316697330039 1109 5 0.1609152719752629 1109 6 0.09535036484209058 1109 7 0.11197895315427786 1109 8 0.08986573177465118 1109 9 0.15976458742026978 1109 10-11 0.13598780773209995 1109 12-13 0.014302975621681924 1109 14-15 0.10121828268007249 1109 16-17 0.1296861948675101 1109 18-19 0.1562491946375104 1109 20-21 0.20233007657067503 1109 22-23 0.11677802964297257 1109 24-25 0.15155509013673907 1109 26-27 0.04172552334611623 1109 28-29 0.18105852826302993 1109 30-31 0.06526466195598601 1109 32-33 0.08356276727051437 1109 34-35 0.04955136645824609 1109 36-37 0.09903937039604926 1109 38-39 0.14121186307088607 1109 40-41 0.16136616923715508 1109 42-43 0.08317108424124342 1109 44-45 0.08081062250958126 1109 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86-87 -1.7507653984261928 2316 88-89 -2.011378031397303 2316 90-91 -2.225284352980939 2316 92-93 -2.2445179078243847 2316 94-95 -2.0280693546103414 2316 96-97 -2.338071730927208 2316 98-99 -2.323760657899289 2316 100-101 -2.1040246605995634 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 6097.0 3 1569.0 4 1545.0 5 1603.0 6 1930.0 7 2436.0 8 3052.0 9 3892.0 10 4988.0 11 6718.0 12 9597.0 13 14075.0 14 12786.0 15 14105.0 16 17906.0 17 25134.0 18 21439.0 19 14560.0 20 14087.0 21 14619.0 22 16124.0 23 17802.0 24 20179.0 25 22870.0 26 26760.0 27 31092.0 28 36224.0 29 43083.0 30 51743.0 31 62740.0 32 78452.0 33 100406.0 34 134185.0 35 196887.0 36 281598.0 37 406958.0 38 351276.0 39 53930.0 40 11.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 47.361355156802865 18.301766242818132 14.455160155624677 19.881718444754327 2 13.971364177709436 22.39081603425143 40.29908755822893 23.338732229810198 3 22.143355895437228 26.958007725078424 31.05146063159442 19.847175747889924 4 11.749464622691516 16.50026975006302 40.45883425566652 31.29143137157894 5 12.11399656660693 40.0018566523051 34.18219413891623 13.701952642171737 6 27.309694109912076 37.39117657170819 18.563646134116148 16.735483184263586 7 25.31328639512589 36.44450849818931 20.844925412710992 17.397279693973815 8 23.415829529735415 32.61789112612089 20.8819291930281 23.084350151115597 9 25.346386357909413 19.988036559144852 22.6162255344102 32.04935154853554 10-11 23.674363783868436 25.891420840501176 27.77713801942589 22.657077356204496 12-13 28.997088624913957 24.248483496909298 26.772970438580774 19.981457439595975 14-15 24.719477107683584 25.318436164646275 29.317697680037412 20.64438904763273 16-17 19.636666691000666 28.93292976571774 30.28730525833406 21.143098284947527 18-19 19.09454020484535 30.254843822492482 31.33401473812625 19.31660123453592 20-21 21.578244673472508 27.520036815315095 29.181342039759695 21.720376471452703 22-23 20.24829888388693 27.232720787064167 29.113817905672146 23.405162423376755 24-25 21.775401588521675 27.083076663590948 30.30532529547742 20.836196452409954 26-27 22.81780893197901 25.101318811107813 31.88445568625548 20.196416570657696 28-29 20.037245070775526 27.18042188781536 32.452747613060176 20.329585428348935 30-31 20.171470429594667 28.58539250947445 30.907003200938377 20.33613385999251 32-33 20.209154753933575 28.22411584506354 30.185492188197355 21.38123721280553 34-35 21.73974020675417 26.30094952788046 30.074080929921553 21.885229335443814 36-37 22.367247751687742 26.002060627677785 31.747928846847962 19.882762773786506 38-39 22.908877573725974 25.49721123771766 31.994990703337507 19.598920485218862 40-41 20.70690998708184 26.95110579841994 32.51501759934028 19.82696661515794 42-43 19.59945597073537 28.698489682623578 30.430779701966372 21.27127464467468 44-45 20.561164106159126 28.47238975342875 29.12204817897915 21.844397961432975 46-47 21.101366354977948 27.796347289202146 28.062887378889222 23.039398976930684 48-49 21.817197799962344 27.18757184988802 29.112462874601068 21.88276747554857 50-51 22.178387329568825 28.246793257757762 29.82591379351534 19.74890561915807 52-53 20.857366076761124 28.682257821497497 30.00814383290927 20.45223226883211 54-55 19.226302791268164 27.5639209349606 30.98277387979605 22.227002393975187 56-57 21.503099682878688 29.158040672209218 28.018664543538975 21.32019510137312 58-59 21.714098331819827 27.81271196839302 28.588748277359688 21.884441422427457 60-61 20.981807147539776 26.705836922732253 30.53680045714912 21.77555547257885 62-63 23.15714394499444 26.534379184385642 29.430807673042896 20.877669197577024 64-65 22.013632662754816 27.781541499526668 30.158670834305145 20.046155003413368 66-67 20.442251270033456 27.460969270797108 30.156037751114606 21.94074170805483 68-69 19.72855734068247 29.27911515078097 29.768926172972236 21.223401335564322 70-71 21.104169185405095 28.65439864592622 27.871296744729225 22.370135423939463 72-73 20.68463100342003 27.344763721587555 28.55654858009634 23.414056694896068 74-75 20.672862873902737 28.93343950536325 28.629046566654885 21.76465105407913 76-77 22.220408997316586 26.553511972251037 29.011653675304398 22.214425355127982 78-79 20.900695325440918 26.8635632207011 30.52418387048337 21.711557583374617 80-81 20.91060265101747 28.95188515852093 29.07880311659615 21.058709073865447 82-83 20.54165053569794 28.329260485444998 28.894697745653268 22.23439123320379 84-85 20.941071353820913 27.256488404108364 28.6155288432772 23.186911398793526 86-87 21.611191350169108 27.554520843537304 29.185090970752803 21.64919683554078 88-89 22.49722746560437 26.11116012205064 29.193102024298966 22.19851038804602 90-91 20.911055558814677 26.451655939942963 30.37802002532024 22.259268475922116 92-93 20.726607336332968 28.48622485912594 29.448994365037684 21.338173439503404 94-95 20.61396950971393 28.640262886451602 28.33401152612304 22.41175607771143 96-97 19.62122366250919 27.51262997830265 28.43344268748149 24.432703671706673 98-99 21.11433550808619 27.447732477720788 27.83858711324683 23.599344900946193 100-101 22.18514711496116 26.70890674527809 28.88428104276814 22.221665096992613 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 8730.0 1 5190.5 2 1789.5 3 1825.5 4 1432.0 5 1408.0 6 2511.5 7 4921.0 8 7442.0 9 7947.0 10 6174.0 11 4540.0 12 4377.5 13 4562.0 14 4727.0 15 5070.0 16 5430.5 17 5341.5 18 5196.5 19 5380.0 20 5644.0 21 6182.5 22 7137.0 23 8429.0 24 9771.0 25 11373.5 26 14489.5 27 18124.5 28 20271.5 29 22365.5 30 25003.0 31 27770.0 32 31156.0 33 34914.0 34 39429.5 35 43061.5 36 46709.0 37 50710.5 38 52117.0 39 52258.5 40 53652.0 41 55405.0 42 56635.5 43 58421.0 44 62699.5 45 66080.0 46 73306.0 47 103740.0 48 218364.5 49 217312.5 50 103976.5 51 71838.5 52 55086.0 53 47388.5 54 42828.0 55 38032.5 56 33685.5 57 29648.5 58 26057.5 59 22807.5 60 19055.0 61 14753.0 62 11077.5 63 8081.5 64 5664.5 65 4012.5 66 2723.0 67 1934.5 68 1298.0 69 752.0 70 489.0 71 315.5 72 216.0 73 154.5 74 97.0 75 66.0 76 55.0 77 40.5 78 34.5 79 24.0 80 20.0 81 16.0 82 10.0 83 8.0 84 7.0 85 8.0 86 7.0 87 3.5 88 3.0 89 4.0 90 3.0 91 2.5 92 2.5 93 2.0 94 2.0 95 1.5 96 0.5 97 0.5 98 0.5 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.3200345688170818 2 0.08482163450630702 3 0.09508307530673706 4 0.09993137073079346 5 0.11104008645969937 6 0.05549650781516979 7 0.05841489923547559 8 0.05879146587035376 9 0.062274707242976804 10-11 0.05933278040799112 12-13 0.05810893884463708 14-15 0.056061357767487045 16-17 0.05782651386847846 18-19 0.06556966529816076 20-21 0.06724067974043263 22-23 0.0816208181098426 24-25 0.09030538612672032 26-27 0.0859748698256214 28-29 0.08289173050255641 30-31 0.07733737263810346 32-33 0.06987664618457978 34-35 0.31885779808308756 36-37 0.4960794706226247 38-39 0.47021404988943066 40-41 0.45216238682995846 42-43 0.4816993322532147 44-45 0.49066632524625103 46-47 0.4862181318717527 48-49 0.5025281742449133 50-51 0.4985506891640127 52-53 0.4984094766759334 54-55 0.4895836961709763 56-57 0.4691784916435157 58-59 0.4522800639033579 60-61 0.4112578361163177 62-63 0.48007538864030264 64-65 0.5041756532725052 66-67 0.5145312357316548 68-69 0.5269108638532746 70-71 0.523968937018289 72-73 0.5122012296783462 74-75 0.5134015358270204 76-77 0.4877008629965855 78-79 0.46979041242519265 80-81 0.47541537653368526 82-83 0.48264074884041014 84-85 0.4840058028918435 86-87 0.48433529869736186 88-89 0.49036036485541257 90-91 0.50525828234778 92-93 0.5115657734819893 94-95 0.5176849812987595 96-97 0.5100595069424767 98-99 0.5123424421664255 100-101 0.4909958210517694 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2124458.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 41.76990265198567 #Duplication Level Percentage of deduplicated Percentage of total 1 71.57572952951932 29.897112546928778 2 14.762399636149423 12.332479914233401 3 5.735386709488951 7.186996335805376 4 2.808642517389108 4.69266898142284 5 1.53997168017504 3.2162233583863116 6 0.9115547901827873 2.284533290873175 7 0.5653023516670355 1.6528836938649447 8 0.3884646500841855 1.2980904494203285 9 0.2882473381416667 1.083605692848427 >10 1.2723423636433127 9.395854799392255 >50 0.08803338429820597 2.511950027867469 >100 0.052180039903824485 4.083108596259985 >500 0.003726781815255737 1.0979262802125864 >1k 0.005194907984901936 5.181268604338993 >5k 0.0014681261696461994 3.9844228221025277 >10k+ 0.0013551933873657226 10.100874606042574 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 38555 1.814815825965964 No Hit GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA 31349 1.4756234295994555 No Hit GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT 22729 1.0698728805182312 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 21502 1.0121169728937922 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 15590 0.7338342297188271 No Hit CTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCA 13330 0.627454155365745 No Hit GCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCT 13172 0.6200169643269013 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 12707 0.5981290286746078 No Hit CTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTG 12613 0.5937043707147893 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 12238 0.5760528097048753 No Hit GTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTG 10326 0.4860533839689935 No Hit TGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCAC 10018 0.4715555685261841 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 9158 0.4310746552767811 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 8071 0.3799086637627103 No Hit TATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGG 7746 0.36461064422078476 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 7714 0.36310437768127213 No Hit GGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGT 7682 0.36159811114175944 No Hit ACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCA 6284 0.29579309169679985 No Hit GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT 6069 0.2856728633844491 No Hit ATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTG 5638 0.2653853359303879 No Hit CAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAG 5613 0.26420856519639363 No Hit TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC 5237 0.24650993335711982 No Hit TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT 5120 0.2410026463220266 No Hit GTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCA 5068 0.23855496319531855 No Hit ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC 5066 0.23846082153659898 No Hit ACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGT 4929 0.23201211791431037 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4915 0.2313531263032736 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 4870 0.2292349389820839 No Hit GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG 4631 0.21798501076509869 No Hit GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA 4580 0.21558439846775038 No Hit ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC 4343 0.20442861190948466 No Hit GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA 3553 0.16724265671526573 No Hit GATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTT 3524 0.16587760266383236 No Hit GTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGC 3471 0.16338284870776454 No Hit CCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATA 3151 0.14832018331263785 No Hit TGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGT 2962 0.13942379656364118 No Hit TGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGAT 2801 0.13184539303671808 No Hit GCTTATACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT 2792 0.13142175557248015 No Hit CCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCAC 2737 0.12883285995769275 No Hit GATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATA 2662 0.12530254775570993 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2592 0.12200758970052597 No Hit AAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGC 2564 0.1206896064784524 No Hit AAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTAC 2546 0.1198423315499765 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2468 0.11617080685991439 No Hit ATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGT 2397 0.11282877797537066 No Hit GTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTG 2386 0.11231099885241319 No Hit GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA 2327 0.1095338199201867 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 4.707082935977082E-5 0.0 4 0.0 0.0 0.0 4.707082935977082E-5 0.0 5 0.0 0.0 0.0 4.707082935977082E-5 0.0 6 0.0 0.0 0.0 4.707082935977082E-5 0.0 7 0.0 0.0 0.0 4.707082935977082E-5 0.0 8 0.0 0.0 0.0 4.707082935977082E-5 0.0 9 0.0 0.0 0.0 4.707082935977082E-5 0.0 10-11 0.0 0.0 0.0 1.1767707339942706E-4 0.0 12-13 0.0 0.0 0.0 3.2949580551839576E-4 0.0 14-15 0.0 0.0 0.0 4.236374642379374E-4 0.0 16-17 0.0 0.0 0.0 4.942437082775936E-4 0.0 18-19 0.0 0.0 0.0 6.589916110367915E-4 0.0 20-21 0.0 0.0 0.0 7.766686844362185E-4 0.0 22-23 0.0 0.0 0.0 0.0013415186367534684 0.0 24-25 0.0 0.0 0.0 0.0024476831267080824 0.0 26-27 0.0 0.0 0.0 0.0033184934698638428 0.0 28-29 0.0 0.0 0.0 0.007766686844362185 0.0 30-31 0.0 0.0 0.0 0.01986388998982329 0.0 32-33 0.0 0.0 0.0 0.04022202368792417 0.0 34-35 0.0 0.0 0.0 0.0754780748783925 0.0 36-37 0.0 0.0 0.0 0.130009630691687 0.0 38-39 0.0 0.0 0.0 0.19677960213852191 0.0 40-41 0.0 0.0 0.0 0.2693863564259684 0.0 42-43 0.0 0.0 0.0 0.34455847091352243 0.0 44-45 0.0 0.0 0.0 0.43637012357975535 0.0 46-47 0.0 0.0 0.0 0.555812353080174 0.0 48-49 0.0 0.0 0.0 0.689705327194042 0.0 50-51 0.0 0.0 0.0 0.8183734392489754 0.0 52-53 0.0 0.0 0.0 0.9461942763754332 0.0 54-55 0.0 0.0 0.0 1.0931258702219577 0.0 56-57 0.0 0.0 0.0 1.2671467263650307 0.0 58-59 0.0 0.0 0.0 1.4828252664915005 0.0 60-61 0.0 0.0 0.0 1.7697455068539836 0.0 62-63 0.0 0.0 0.0 2.0309886098007115 0.0 64-65 0.0 0.0 0.0 2.2587878884873227 0.0 66-67 0.0 0.0 0.0 2.5212548329974043 0.0 68-69 0.0 0.0 0.0 2.832934329603127 0.0 70-71 0.0 0.0 0.0 3.1869305018032836 0.0 72-73 0.0 0.0 0.0 3.573311404602962 0.0 74-75 0.0 0.0 0.0 3.96397575287438 0.0 76-77 0.0 0.0 0.0 4.332563882176066 0.0 78-79 0.0 0.0 2.353541467988541E-5 4.785079300226222 0.0 80-81 0.0 0.0 4.707082935977082E-5 5.288219395252813 0.0 82-83 0.0 0.0 4.707082935977082E-5 5.789170696714174 0.0 84-85 0.0 0.0 4.707082935977082E-5 6.422791130726049 0.0 86-87 0.0 0.0 4.707082935977082E-5 7.095809848912052 0.0 88-89 0.0 0.0 4.707082935977082E-5 7.698528283449237 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CTTTGCG 1185 0.0 29.976955 2 TTTGCGT 1205 0.0 29.87247 3 TTTCGCG 35 0.008334638 27.182423 68-69 TTGCGTT 1495 0.0 24.077808 4 GAGTACT 10685 0.0 22.552969 12-13 TACCTGG 1410 0.0 22.17019 2 GCTTTGC 1665 0.0 21.94887 1 ATGGGAT 3135 0.0 21.906605 5 GTACATG 15505 0.0 21.243368 1 TACATGG 15285 0.0 21.226076 2 GTGTAGC 1125 0.0 21.09372 1 GTACTTT 11335 0.0 20.9678 14-15 ATGGGAG 3115 0.0 20.678806 5 AGTACTT 10695 0.0 20.606268 12-13 GTAAGCG 335 0.0 20.58748 94-95 ACATGGG 15505 0.0 19.79465 3 GTATAGG 410 0.0 19.678898 1 GTAGCCC 1080 0.0 19.296276 3 CATGGGG 2935 0.0 19.203634 4 CATGGGA 11480 0.0 18.937183 4 >>END_MODULE