##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938872_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2136560 Sequences flagged as poor quality 0 Sequence length 101 %GC 43 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.11368133822593 33.0 31.0 34.0 30.0 34.0 2 32.33529130939454 34.0 31.0 34.0 30.0 34.0 3 32.425922042910095 34.0 31.0 34.0 30.0 34.0 4 35.96795035009548 37.0 35.0 37.0 35.0 37.0 5 35.90187216834538 37.0 35.0 37.0 35.0 37.0 6 35.97206490807653 37.0 35.0 37.0 35.0 37.0 7 35.92675375369753 37.0 35.0 37.0 35.0 37.0 8 35.91666229827386 37.0 35.0 37.0 35.0 37.0 9 37.557464803235106 39.0 37.0 39.0 35.0 39.0 10-11 37.47961793724492 39.0 37.0 39.0 34.0 39.0 12-13 37.40856142584341 39.0 37.0 39.0 34.0 39.0 14-15 38.51192501029693 40.0 38.0 41.0 34.0 41.0 16-17 38.529111281686454 40.0 38.0 41.0 34.0 41.0 18-19 38.75214644100798 40.0 38.0 41.0 34.0 41.0 20-21 38.7600270996368 40.0 38.0 41.0 34.0 41.0 22-23 38.72385750926723 40.0 38.0 41.0 34.0 41.0 24-25 38.64189070281199 40.0 38.0 41.0 34.0 41.0 26-27 38.38647803010447 40.0 38.0 41.0 34.0 41.0 28-29 38.19467157486801 40.0 38.0 41.0 33.5 41.0 30-31 38.06657664657206 40.0 37.0 41.0 33.0 41.0 32-33 37.8866895851275 40.0 37.0 41.0 33.0 41.0 34-35 37.841948974051746 40.0 37.0 41.0 33.0 41.0 36-37 37.67731938255888 40.0 37.0 41.0 33.0 41.0 38-39 37.4816939379189 40.0 36.5 41.0 32.5 41.0 40-41 37.31345761410866 40.0 36.0 41.0 32.0 41.0 42-43 37.19527183884375 40.0 36.0 41.0 31.5 41.0 44-45 36.953534185793984 40.0 36.0 41.0 31.0 41.0 46-47 36.57182573857041 40.0 36.0 41.0 30.0 41.0 48-49 36.222571563635 39.5 35.5 41.0 29.5 41.0 50-51 35.95185134047254 39.0 35.0 41.0 28.5 41.0 52-53 35.6441305182162 39.0 35.0 40.5 28.0 41.0 54-55 35.45367132212529 39.0 35.0 40.0 27.5 41.0 56-57 35.256055294492086 38.5 35.0 40.0 27.0 41.0 58-59 35.01058711199311 38.0 34.5 40.0 26.5 41.0 60-61 34.539885142472016 38.0 34.0 40.0 26.0 41.0 62-63 34.19033680308533 37.5 34.0 40.0 25.0 41.0 64-65 33.86101466843898 37.0 33.0 39.5 24.5 41.0 66-67 33.390943853671324 36.0 33.0 39.0 22.5 40.5 68-69 33.00778307185382 36.0 32.5 39.0 22.0 40.0 70-71 32.49656901748605 35.5 32.0 38.0 21.5 40.0 72-73 31.913432105814955 35.0 31.0 37.0 20.0 39.5 74-75 31.44179709439473 35.0 31.0 37.0 18.5 39.0 76-77 29.41673390871307 32.5 28.5 35.0 13.0 37.0 78-79 30.352620801662486 34.0 30.0 36.0 12.5 37.5 80-81 30.39887061444565 34.0 30.5 35.5 12.0 37.0 82-83 30.097857069307672 34.0 30.0 35.0 7.5 37.0 84-85 29.70898055753173 34.0 30.0 35.0 4.5 36.0 86-87 29.12321535552477 34.0 29.0 35.0 2.0 36.0 88-89 28.469651215037256 34.0 28.5 35.0 2.0 35.5 90-91 27.931416154940653 33.0 27.0 35.0 2.0 35.0 92-93 27.6585874021792 33.0 27.0 35.0 2.0 35.0 94-95 27.306354373385254 33.0 26.5 35.0 2.0 35.0 96-97 26.600442065750553 33.0 25.0 35.0 2.0 35.0 98-99 25.68361735687273 32.5 24.0 34.0 2.0 35.0 100-101 21.438581645261543 27.5 2.0 32.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.1783578875952614 1101 2 0.17125258319060777 1101 3 0.17775709485115243 1101 4 0.13150878802807853 1101 5 0.12908176726427456 1101 6 0.09840893129020145 1101 7 0.07353682361753755 1101 8 0.14040585285175666 1101 9 0.18355677517157432 1101 10-11 0.2493535529374853 1101 12-13 0.2724167901208929 1101 14-15 0.17270418572451973 1101 16-17 0.24353371589251793 1101 18-19 0.32906377544987464 1101 20-21 0.340229938452417 1101 22-23 0.3345523565588451 1101 24-25 0.3126291875414253 1101 26-27 0.3157643208729439 1101 28-29 0.38260582802303844 1101 30-31 0.33780355291085584 1101 32-33 0.3577564794591197 1101 34-35 0.43878253952340174 1101 36-37 0.4863954768067771 1101 38-39 0.549698888643249 1101 40-41 0.5772428232608817 1101 42-43 0.5073349858751754 1101 44-45 0.6519832275099517 1101 46-47 0.5806468516370842 1101 48-49 0.2915361155933738 1101 50-51 0.39561821098092764 1101 52-53 0.3814733182175871 1101 54-55 0.4719769208439075 1101 56-57 0.46857520460009994 1101 58-59 0.44372353908849504 1101 60-61 0.49146954304706725 1101 62-63 0.2035949217435089 1101 64-65 0.22702675278143403 1101 66-67 0.3044429703927918 1101 68-69 0.22515710557093627 1101 70-71 0.29256800590562193 1101 72-73 0.2534289817225819 1101 74-75 0.160477290323918 1101 76-77 0.04817789315107035 1101 78-79 0.1966295417260966 1101 80-81 0.44568077375166837 1101 82-83 0.4339674720287299 1101 84-85 0.2848605794118697 1101 86-87 0.3614943456918276 1101 88-89 0.5217418403460385 1101 90-91 0.5858970428904833 1101 92-93 0.6693993586481994 1101 94-95 0.5884318518789762 1101 96-97 0.5547925372969438 1101 98-99 0.526216202367376 1101 100-101 0.8188440789477376 1102 1 0.1067788475874707 1102 2 0.12245762919787495 1102 3 0.17364930263849487 1102 4 0.13375484922880077 1102 5 0.05604498766101784 1102 6 0.090345955441677 1102 7 0.1307725299050091 1102 8 0.12958421950931154 1102 9 0.21452414502360995 1102 10-11 0.219149225969403 1102 12-13 0.22950639476169243 1102 14-15 0.2581617118552302 1102 16-17 0.250427784942751 1102 18-19 0.17890606431637934 1102 20-21 0.17348891801596977 1102 22-23 0.23108842274041308 1102 24-25 0.2395966210583893 1102 26-27 0.14260976115336632 1102 28-29 0.13760937640650894 1102 30-31 0.2943566869105467 1102 32-33 0.28395548666075143 1102 34-35 0.3215302335422692 1102 36-37 0.3094748557366813 1102 38-39 0.40256900657317374 1102 40-41 0.4072885964714388 1102 42-43 0.2440844887269975 1102 44-45 0.21247871853506695 1102 46-47 0.36786672595502523 1102 48-49 0.39281461032977916 1102 50-51 0.4246072868283264 1102 52-53 0.45109981106651986 1102 54-55 0.4113625262037459 1102 56-57 0.38309474037042435 1102 58-59 0.29062881503850946 1102 60-61 0.2986097519429336 1102 62-63 0.37796215789919785 1102 64-65 0.45666638120295744 1102 66-67 0.3638070045771187 1102 68-69 0.41947182752372925 1102 70-71 0.38909714384504923 1102 72-73 0.5463772951637473 1102 74-75 0.6611534961189278 1102 76-77 0.3269193311545422 1102 78-79 0.41633346236214663 1102 80-81 0.5596639325057815 1102 82-83 0.42542981796896484 1102 84-85 0.3443840099777802 1102 86-87 0.45188871246922346 1102 88-89 0.5245505871581102 1102 90-91 0.6754998111913437 1102 92-93 0.6197842505863456 1102 94-95 0.4904203978094763 1102 96-97 0.4540081479372269 1102 98-99 0.7701467345687192 1102 100-101 1.0956666747540886 1103 1 0.013969430733091315 1103 2 0.02013177515596709 1103 3 0.030948852268409155 1103 4 0.022107414076948828 1103 5 0.09887694850629458 1103 6 0.005161412139372601 1103 7 0.04702681685456156 1103 8 0.1161305774152126 1103 9 0.03531748961117387 1103 10-11 0.09960618084851092 1103 12-13 0.05600930045736874 1103 14-15 0.16080318176977215 1103 16-17 0.05725856784712846 1103 18-19 0.15336057156219596 1103 20-21 0.24423824042191455 1103 22-23 0.2527372573342461 1103 24-25 0.1162906061918676 1103 26-27 0.06944929752101814 1103 28-29 0.18712819079184584 1103 30-31 0.1496183570750489 1103 32-33 0.21062495838993556 1103 34-35 0.14757021124388103 1103 36-37 -0.012378149101586189 1103 38-39 0.16083046404102674 1103 40-41 0.21746993078838983 1103 42-43 0.32736113384750354 1103 44-45 0.38566603569820757 1103 46-47 0.48677677844337097 1103 48-49 0.39144181335035455 1103 50-51 0.24738267221935928 1103 52-53 0.264594973028764 1103 54-55 0.30731436222992414 1103 56-57 0.20046130566250042 1103 58-59 0.1826991689722135 1103 60-61 0.2708603697594967 1103 62-63 0.2781022411150289 1103 64-65 0.27123417837469077 1103 66-67 0.4696293783180252 1103 68-69 0.5383712220102055 1103 70-71 0.49329104440960236 1103 72-73 0.6891246639116098 1103 74-75 0.5560108016926364 1103 76-77 0.4423591614050082 1103 78-79 0.5683728710191325 1103 80-81 0.6489677935291489 1103 82-83 0.6852025105926565 1103 84-85 0.6361864310124083 1103 86-87 0.5896343504502646 1103 88-89 0.9882159527762298 1103 90-91 1.1218883240896105 1103 92-93 0.921093145893721 1103 94-95 0.7286685605784804 1103 96-97 0.7051398655483716 1103 98-99 0.821662881673241 1103 100-101 1.2933480496177943 1104 1 -0.12326618279823265 1104 2 -0.06760683511132726 1104 3 -0.03319873192711498 1104 4 -0.04070939073630342 1104 5 -0.011932891825580327 1104 6 0.028234720464311636 1104 7 0.013544195152213945 1104 8 0.033949928097399606 1104 9 0.04161554216576491 1104 10-11 -0.029274595127716907 1104 12-13 -0.06570979032681379 1104 14-15 -0.07925043734744719 1104 16-17 -0.011688643185202352 1104 18-19 0.02010794400607807 1104 20-21 -0.030647484123143443 1104 22-23 -0.010039829614392204 1104 24-25 0.0675324244849449 1104 26-27 0.04005819316810744 1104 28-29 -0.16572589570304075 1104 30-31 -0.24768140542597905 1104 32-33 -0.07112722291750373 1104 34-35 -0.01626124645948579 1104 36-37 -0.06922018630476146 1104 38-39 0.057071006513162104 1104 40-41 0.06641261583150282 1104 42-43 -0.22606378149225037 1104 44-45 -0.1402648806183322 1104 46-47 -0.01742500216391818 1104 48-49 0.002627126053482698 1104 50-51 -0.0789089377281158 1104 52-53 -0.05029403054930981 1104 54-55 -0.02952176347711344 1104 56-57 -0.008364488781388957 1104 58-59 -0.021510693758685306 1104 60-61 0.06357294981582129 1104 62-63 0.13880787978175846 1104 64-65 0.060262926488384494 1104 66-67 0.08032907085094365 1104 68-69 0.17142468294873225 1104 70-71 -0.013720659659981038 1104 72-73 -0.1554951241289224 1104 74-75 -0.20327353780735535 1104 76-77 -0.28956972976227036 1104 78-79 -0.15728810236874224 1104 80-81 -0.21414366241923943 1104 82-83 -0.09631367270528912 1104 84-85 0.004568470686400872 1104 86-87 -0.24532175784557708 1104 88-89 -0.10361763860776207 1104 90-91 -0.17368128666376847 1104 92-93 -0.14891120690714033 1104 94-95 -0.006409356866090121 1104 96-97 0.01362251611544707 1104 98-99 0.08190056538279933 1104 100-101 0.39994029147188215 1105 1 -0.015174293186682064 1105 2 -0.026894043162386083 1105 3 0.033321974367680696 1105 4 0.013248120582957768 1105 5 0.013159207589900745 1105 6 0.009705423487964993 1105 7 0.028755882810550304 1105 8 0.026232527229758773 1105 9 -0.05255642035829311 1105 10-11 0.009113208270207451 1105 12-13 0.023469853728407486 1105 14-15 0.046381850801331836 1105 16-17 0.12704973581996626 1105 18-19 0.03228491926500965 1105 20-21 0.06745672300973382 1105 22-23 -0.003061254431266036 1105 24-25 0.0510342777530397 1105 26-27 -0.004706521870780023 1105 28-29 0.0017543815991061251 1105 30-31 -0.009579093296380847 1105 32-33 -0.10162290163322751 1105 34-35 0.047597205819144506 1105 36-37 0.07921011930964283 1105 38-39 0.061960227779607635 1105 40-41 0.02448600660147804 1105 42-43 -0.02748108603055499 1105 44-45 -0.03591393052234082 1105 46-47 0.02597745367009452 1105 48-49 -0.16510525436390822 1105 50-51 -0.04360288341823093 1105 52-53 -0.024776796171245508 1105 54-55 0.026924970010973936 1105 56-57 -0.06007416525469722 1105 58-59 -0.13404004938989544 1105 60-61 -0.21738159040901195 1105 62-63 -0.13820234646810547 1105 64-65 -0.20427016951835242 1105 66-67 0.019701937562281557 1105 68-69 -0.12229394515913583 1105 70-71 0.03904299959135926 1105 72-73 0.06103313961943613 1105 74-75 -0.02990668096357041 1105 76-77 -0.22735598241583688 1105 78-79 0.1114318229139819 1105 80-81 0.0706057346764446 1105 82-83 0.057932939412353335 1105 84-85 -0.10930092298022842 1105 86-87 -0.26574493012618916 1105 88-89 -0.18080326545905478 1105 90-91 -0.048229813411513334 1105 92-93 -0.04168876284921552 1105 94-95 -0.005058828633924151 1105 96-97 -0.013690685063966868 1105 98-99 0.2377131495581679 1105 100-101 0.7899082794521455 1106 1 0.023765208021636397 1106 2 -0.04777445359385979 1106 3 -0.08090796847414339 1106 4 -0.06355701704013228 1106 5 -0.10650001226230899 1106 6 -0.03243539386597405 1106 7 -0.04700095822835948 1106 8 -0.04528924850882987 1106 9 -0.1116780553835639 1106 10-11 0.028329480936641005 1106 12-13 0.0407969443042262 1106 14-15 0.07796740599736296 1106 16-17 -0.008757791989872032 1106 18-19 -0.06699489481002985 1106 20-21 -0.08564659636100203 1106 22-23 0.06823966487811362 1106 24-25 0.025196059275337745 1106 26-27 -0.014400620287219112 1106 28-29 -0.04919115723327394 1106 30-31 0.06371217886310632 1106 32-33 0.0460081596855062 1106 34-35 0.11189486033268281 1106 36-37 -0.022593232947109243 1106 38-39 0.06404668183044748 1106 40-41 0.06037605801121515 1106 42-43 0.06527795299270167 1106 44-45 -0.031318485719253886 1106 46-47 0.040235036627905174 1106 48-49 -0.042671442963836625 1106 50-51 0.03676725608721654 1106 52-53 0.1742725954376212 1106 54-55 0.0753972254179871 1106 56-57 0.02881173088018585 1106 58-59 0.05188138920100016 1106 60-61 0.1538516172286606 1106 62-63 0.04872440766535391 1106 64-65 -0.008427163613859534 1106 66-67 0.10892793496490327 1106 68-69 -0.07018439155361733 1106 70-71 0.08989331888539454 1106 72-73 0.10624985664372488 1106 74-75 0.25925896664831427 1106 76-77 0.07409012023963868 1106 78-79 0.15625487768020818 1106 80-81 0.18004318117055718 1106 82-83 0.31100553284872845 1106 84-85 0.19835627081181073 1106 86-87 0.22744136249226798 1106 88-89 0.2720454298128523 1106 90-91 0.5321817642263547 1106 92-93 0.5162977318462652 1106 94-95 0.4420978542347562 1106 96-97 0.5050239217013832 1106 98-99 0.45972213193461897 1106 100-101 0.9692767623139495 1107 1 -0.015392874152020397 1107 2 -0.034448060588168516 1107 3 -0.09997437172383172 1107 4 -0.07924417300866082 1107 5 -0.13969177868523275 1107 6 -0.09449458557192969 1107 7 -0.10142447507289631 1107 8 -0.0113526236900654 1107 9 0.07331125151693385 1107 10-11 0.025014203847128158 1107 12-13 0.034116659873475896 1107 14-15 -0.012509776580131415 1107 16-17 -0.029394422460796932 1107 18-19 -0.046415443174772975 1107 20-21 -0.07579902067791267 1107 22-23 -0.11035262285805914 1107 24-25 -0.06194514353077096 1107 26-27 -0.022088106622362602 1107 28-29 0.03621375874450905 1107 30-31 0.009603145614818231 1107 32-33 0.04330936245655437 1107 34-35 -0.09936133020735127 1107 36-37 -0.009688232625066462 1107 38-39 0.055700433966990204 1107 40-41 -0.0483189369346988 1107 42-43 -0.03356913765613001 1107 44-45 -0.11655986146395492 1107 46-47 -0.08072930962958935 1107 48-49 -0.18225352482552637 1107 50-51 -0.28935858642431356 1107 52-53 -0.1561449458899702 1107 54-55 -0.34478977347304607 1107 56-57 -0.1965647840851119 1107 58-59 -0.2309409055499856 1107 60-61 -0.26438067606327564 1107 62-63 -0.4289794611729789 1107 64-65 -0.3069129541255009 1107 66-67 -0.18873029596135638 1107 68-69 -0.36603382149596797 1107 70-71 -0.289984024844415 1107 72-73 -0.10961306399862636 1107 74-75 -0.2592467115824988 1107 76-77 -0.5825618157568684 1107 78-79 -0.14862070248780768 1107 80-81 0.007522146588531342 1107 82-83 0.029253643427779252 1107 84-85 0.08141470758187808 1107 86-87 0.16181900920417647 1107 88-89 0.26110466489585704 1107 90-91 0.33184136393944996 1107 92-93 0.2874172058184996 1107 94-95 0.14758380204296273 1107 96-97 0.18162141293067435 1107 98-99 0.2444612321369135 1107 100-101 0.7634379926097061 1108 1 -0.034238677786682103 1108 2 -0.06574399723452728 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-1.3351090968890773 2316 56-57 -1.592999076193422 2316 58-59 -1.3897012233022679 2316 60-61 -1.2314972235536814 2316 62-63 -1.2158595338283433 2316 64-65 -1.2383532625276175 2316 66-67 -1.4257796487307672 2316 68-69 -1.4964557667898433 2316 70-71 -1.4130277713476822 2316 72-73 -1.1466698892348681 2316 74-75 -1.6017324755751687 2316 76-77 -1.4217310498864961 2316 78-79 -1.4430838919665767 2316 80-81 -1.5635947572810558 2316 82-83 -1.841510138483489 2316 84-85 -1.89809693797028 2316 86-87 -1.8664980954640065 2316 88-89 -2.22152855577567 2316 90-91 -2.251720167034879 2316 92-93 -2.44797163069925 2316 94-95 -2.6434317061997383 2316 96-97 -2.955585723849957 2316 98-99 -3.2530711293925307 2316 100-101 -3.8160079335546584 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 2.0 3 3.0 4 15.0 5 62.0 6 179.0 7 331.0 8 766.0 9 1335.0 10 2253.0 11 3449.0 12 5070.0 13 7359.0 14 8776.0 15 12126.0 16 16061.0 17 23020.0 18 22809.0 19 14802.0 20 12824.0 21 13050.0 22 13913.0 23 15248.0 24 17557.0 25 20913.0 26 24737.0 27 29965.0 28 35976.0 29 43696.0 30 53220.0 31 64775.0 32 79498.0 33 101620.0 34 137594.0 35 198755.0 36 302292.0 37 469429.0 38 354394.0 39 28684.0 40 2.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 45.86866995222038 19.186092409017604 15.105558750888115 19.8396788878739 2 14.339499007750778 23.213015314337067 39.651355449882054 22.796130228030105 3 22.15795484329951 26.997135582431568 31.216909424495448 19.628000149773467 4 11.942749091998353 17.12626839405399 39.80318830269218 31.127794211255477 5 11.926252967989939 40.46997327931471 34.04259302510078 13.561180727594571 6 27.316012655858014 37.99135058224435 17.959617328790205 16.733019433107422 7 25.345181038678998 36.300642153742466 20.604850788182873 17.749326019395664 8 23.65756168794698 33.13082712397499 20.432658104616767 22.778953083461264 9 25.08827273748456 19.98198037967574 22.297478189238777 32.63226869360093 10-11 23.876464971730258 26.260062904856408 27.51876848766241 22.344703635750925 12-13 29.058392930692328 24.36992174336316 26.767490732766692 19.80419459317782 14-15 24.412354583472773 25.682120817117436 29.152248851528288 20.7532757478815 16-17 19.55642457708334 29.22804911297787 30.159337515401923 21.056188794536865 18-19 19.122140262852437 30.37656326056839 31.184918747893807 19.316377728685364 20-21 21.55586550342607 27.572546523383384 29.182681506721085 21.688906466469465 22-23 20.339353914704013 27.1977618227431 29.162625903321228 23.300258359231663 24-25 21.892130340360204 27.3062071741491 30.2261345340173 20.575527951473397 26-27 22.75353839817276 25.32217677013517 31.968655221477515 19.95562961021455 28-29 20.099739768599992 27.346482195679034 32.28451342344704 20.269264612273936 30-31 20.180898266372115 28.742136892949414 30.781092035795858 20.295872804882613 32-33 20.288360729396786 28.285070393529786 30.10764967985921 21.318919197214214 34-35 21.736754554628394 26.456184577825127 30.08729665416652 21.719764213379964 36-37 22.377160520987115 26.184933949820397 31.70506916756459 19.732836361627893 38-39 22.848743099004533 25.575601768677043 32.14389365468856 19.43176147762987 40-41 20.696170819444777 27.04159112455573 32.414416746255505 19.847821309743992 42-43 19.570890920519737 28.814678665489062 30.413663567735355 21.200766846255846 44-45 20.619466251417588 28.499229364737243 29.03205121904529 21.84925316479987 46-47 21.202810694230052 27.937276567781577 27.98939320045269 22.870519537535678 48-49 21.95092099197072 27.270629588285818 29.07465522769131 21.703794192052158 50-51 22.176613864982205 28.409027084811573 29.64234831138828 19.772010738817944 52-53 20.96704515670049 28.900966974950386 29.808945220354214 20.323042647994907 54-55 19.189772344329203 27.748319728910026 30.731573183060622 22.330334743700153 56-57 21.559703448534094 29.28059591118433 27.816817688246527 21.342882952035048 58-59 21.832797111610567 27.953119836896235 28.359311302218433 21.854771749274768 60-61 21.013585982892142 27.041636018850422 30.231731424732043 21.71304657352539 62-63 23.20033137379713 26.701028756505785 29.26774815591418 20.830891713782904 64-65 21.898549181450022 28.011691732358557 29.91978900588682 20.1699700803046 66-67 20.380378820216723 27.671132791145258 29.94449405764867 22.003994330989343 68-69 19.762254127613765 29.448918220006785 29.466376475819377 21.32245117656007 70-71 21.118105740068145 28.84428707829408 27.644999438349497 22.392607743288277 72-73 20.71610439210694 27.480974089190102 28.30416651065264 23.498755008050324 74-75 20.707515819822518 29.222465084060357 28.213202531171603 21.856816564945518 76-77 22.094940769215267 26.858919717086838 28.828561801849258 22.21757771184864 78-79 20.782730183098064 27.106540420114577 30.14616954356536 21.964559853222003 80-81 20.67898865465983 29.28733571722769 28.78627326169169 21.24740236642079 82-83 20.451496798592128 28.6393782528925 28.600624368143183 22.308500580372186 84-85 20.69799116336541 27.62414816340285 28.363467442992473 23.314393230239265 86-87 21.371602136009475 27.93337450358404 28.752248446448007 21.942774913958473 88-89 22.19554109091405 26.525805650157874 28.948779357830407 22.329873901097674 90-91 20.586945179031922 26.895740551060364 30.01820305422453 22.49911121568318 92-93 20.286137529486652 28.98654847043846 29.09213876511776 21.635175234957128 94-95 20.143922941550905 29.16124517916651 27.92877335530011 22.766058523982476 96-97 19.190363536755374 28.19442920051307 27.90878197881025 24.70642528392131 98-99 20.649642415846035 28.048124087317934 27.354766540607333 23.947466956228705 100-101 21.439368179296068 27.637756192151343 28.37927683132968 22.543598797222913 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 7294.0 1 4513.5 2 1754.5 3 1728.5 4 1393.0 5 1328.0 6 2353.0 7 4769.0 8 7096.0 9 7475.5 10 5791.5 11 4040.5 12 3701.0 13 3920.5 14 4165.5 15 4231.0 16 4366.0 17 4586.0 18 4772.0 19 5021.5 20 5410.0 21 6192.0 22 7268.5 23 8556.5 24 9941.5 25 11673.0 26 14911.5 27 18537.0 28 20436.0 29 22487.0 30 25422.5 31 28425.5 32 31650.5 33 35153.0 34 40137.5 35 43781.5 36 47201.5 37 51300.0 38 52654.5 39 52780.5 40 54010.0 41 56025.5 42 57251.5 43 58973.0 44 63622.5 45 66775.5 46 73115.0 47 105924.0 48 226572.5 49 223512.0 50 105447.0 51 72917.0 52 54565.5 53 45943.5 54 41664.5 55 37222.0 56 32983.5 57 29085.5 58 25736.0 59 22655.0 60 18916.5 61 14555.0 62 10643.0 63 7607.5 64 5365.0 65 3790.5 66 2500.0 67 1756.5 68 1183.5 69 669.0 70 404.0 71 231.0 72 139.5 73 82.5 74 55.5 75 37.0 76 21.0 77 10.0 78 4.0 79 2.5 80 3.0 81 3.5 82 2.5 83 1.5 84 0.5 85 0.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.5 91 0.5 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.39577638821282807 2 0.0 3 0.0 4 0.0 5 6.084547122477253E-4 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0011701052158610101 16-17 0.0026912419964803236 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0024338188489909014 36-37 0.007207848129703823 38-39 0.012824353165836671 40-41 0.0027380462051147638 42-43 7.95671546785487E-4 44-45 5.148462949788444E-4 46-47 2.8082525180664244E-4 48-49 2.3402104317220206E-4 50-51 5.616505036132849E-4 52-53 0.0 54-55 0.0 56-57 0.0 58-59 2.8082525180664244E-4 60-61 0.002644437787845883 62-63 0.0 64-65 3.510315647583031E-4 66-67 0.001497734676302093 68-69 0.0016381473022054145 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.12258022241359942 78-79 0.0 80-81 0.0 82-83 0.0 84-85 0.0 86-87 0.14708222563372897 88-89 0.43904687909536827 90-91 0.3391900999737896 92-93 0.0 94-95 0.0 96-97 3.978357733927435E-4 98-99 0.0 100-101 2.1061893885498183E-4 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2136560.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 40.40580096033953 #Duplication Level Percentage of deduplicated Percentage of total 1 70.26378365539286 28.390644571001616 2 15.428064134442288 12.46766577239256 3 5.950252098520526 7.21274105869989 4 2.9097433816016145 4.702820476906404 5 1.6306188621679165 3.294323059346608 6 0.9305217907717551 2.2559086960309345 7 0.6331980279853937 1.7909411441080123 8 0.42099800885923483 1.3608609400532399 9 0.3050937321849183 1.109480095521985 >10 1.3658510872704168 9.721273777474947 >50 0.09388333343121336 2.6183838979960954 >100 0.05522977172175126 4.1998665093465695 >500 0.0042927116277981184 1.2095590682479 >1k 0.005336884726451715 4.909913500158139 >5k 0.001740288497755994 4.651706719757029 >10k+ 0.0013922307982047952 10.10391071295802 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 39856 1.865428539334257 No Hit GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA 33255 1.5564739581383158 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 23322 1.091567753772419 No Hit GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT 19848 0.9289699329763732 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 15007 0.7023907589770473 No Hit CTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTG 13600 0.6365372374283895 No Hit CTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCA 13169 0.6163646235069458 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 12684 0.5936645823192421 No Hit GCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCT 12293 0.575364136743176 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 11545 0.5403545886846145 No Hit TGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCAC 10547 0.493643988467443 No Hit GTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTG 10408 0.4871382034672558 No Hit TATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGG 8966 0.41964653461639273 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 8738 0.40897517504774034 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 8565 0.4008780469539821 No Hit GGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGT 8044 0.37649305425543866 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 7213 0.3375987568802187 No Hit ACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCA 6486 0.30357209720298045 No Hit GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT 6464 0.30254240461302284 No Hit CAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAG 6186 0.2895308346126484 No Hit ATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTG 5965 0.27918710450443707 No Hit TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC 5951 0.27853184558355487 No Hit ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC 5606 0.2623843936046729 No Hit GTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCA 5445 0.254848916014528 No Hit GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA 5240 0.24525405324446772 No Hit TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT 5190 0.2429138428127457 No Hit ACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGT 5170 0.24197775864005694 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 4980 0.23308495899951326 No Hit GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG 4957 0.2320084622009211 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4713 0.22058823529411764 No Hit ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC 4240 0.19844984461002735 No Hit GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA 3985 0.18651477140824502 No Hit GATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTT 3695 0.1729415509042573 No Hit GTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGC 3494 0.1635339049687348 No Hit TGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGT 3216 0.15052233496836034 No Hit CCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATA 3206 0.15005429288201597 No Hit AAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGC 3015 0.14111468903283783 No Hit GCTTATACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT 2874 0.13451529561538175 No Hit GATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATA 2824 0.13217508518365972 No Hit TGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGAT 2792 0.13067735050735763 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2737 0.1281031190324634 No Hit AAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTAC 2693 0.12604373385254802 No Hit ATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGT 2649 0.12398434867263264 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2560 0.11981877410416746 No Hit CCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCAC 2452 0.11476391957164789 No Hit GTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTG 2409 0.11275133860036694 No Hit GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA 2252 0.10540307784475979 No Hit GCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAAC 2195 0.10273523795259669 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 1.1701052158610102E-4 0.0 12-13 0.0 0.0 0.0 2.1061893885498183E-4 0.0 14-15 0.0 0.0 0.0 3.2762946044108285E-4 0.0 16-17 0.0 0.0 0.0 5.850526079305052E-4 0.0 18-19 0.0 0.0 0.0 7.488673381510465E-4 0.0 20-21 0.0 0.0 0.0 0.0010062904856404688 0.0 22-23 0.0 0.0 0.0 0.0017785599281087356 0.0 24-25 0.0 0.0 0.0 0.0028082525180664246 0.0 26-27 0.0 0.0 0.0 0.0038145430037068934 0.0 28-29 0.0 0.0 0.0 0.009290635413936422 0.0 30-31 0.0 0.0 0.0 0.021646946493428687 0.0 32-33 0.0 0.0 0.0 0.04385554349047066 0.0 34-35 0.0 0.0 0.0 0.08239880930093235 0.0 36-37 0.0 0.0 0.0 0.14041262590332124 0.0 38-39 0.0 0.0 0.0 0.21040831991612685 0.0 40-41 0.0 0.0 0.0 0.28768206837158794 0.0 42-43 0.0 0.0 0.0 0.37047871344591304 0.0 44-45 0.0 0.0 0.0 0.47150559778335266 0.0 46-47 0.0 0.0 0.0 0.5997725315460367 0.0 48-49 0.0 0.0 0.0 0.7443273299135058 0.0 50-51 0.0 0.0 0.0 0.8868929494140113 0.0 52-53 0.0 0.0 0.0 1.0186936009285956 0.0 54-55 0.0 0.0 0.0 1.162101696184521 0.0 56-57 0.0 0.0 0.0 1.3417596510278205 0.0 58-59 0.0 0.0 0.0 1.5653199535702251 0.0 60-61 0.0 0.0 0.0 1.857354813344816 0.0 62-63 0.0 0.0 0.0 2.1166033249709812 0.0 64-65 0.0 0.0 0.0 2.3465524019919872 0.0 66-67 0.0 0.0 0.0 2.6196783614782637 0.0 68-69 0.0 0.0 0.0 2.9333835698506006 0.0 70-71 0.0 0.0 0.0 3.2899848354364023 0.0 72-73 0.0 0.0 0.0 3.677196053469128 0.0 74-75 0.0 0.0 0.0 4.083082150746995 0.0 76-77 0.0 0.0 0.0 4.465636350020594 0.0 78-79 0.0 0.0 0.0 4.925464297749654 0.0 80-81 0.0 0.0 0.0 5.438204403339949 0.0 82-83 0.0 0.0 0.0 5.941560265099038 0.0 84-85 0.0 0.0 0.0 6.562675515782379 0.0 86-87 0.0 0.0 0.0 7.233052196053469 0.0 88-89 0.0 0.0 0.0 7.837879582132025 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CTTTGCG 1055 0.0 34.63207 2 TTTGCGT 1055 0.0 34.63207 3 TTGCGTT 1240 0.0 29.847855 4 GAGTACT 10990 0.0 23.425179 12-13 GTACTTT 11750 0.0 21.80905 14-15 AGTACTT 11085 0.0 21.55483 12-13 GCTTTGC 1760 0.0 21.379827 1 TACCTGG 1370 0.0 20.434858 2 TACGTCC 120 0.0047936765 19.771013 4 ACTTTTT 13615 0.0 18.959705 16-17 TAGGACA 785 0.0 18.73839 4 GTACATG 14655 0.0 18.688389 1 GTATAGC 720 0.0 18.52317 1 TACATGG 14455 0.0 18.51404 2 CGTACTC 185 1.5343248E-4 17.95422 3 TCGTTCG 135 0.00945966 17.574234 5 ACATGGG 14620 0.0 17.428785 3 TACTTTT 13600 0.0 17.411753 14-15 GGCTACG 165 0.0014375998 17.320366 1 ATGGGAG 2935 0.0 17.137125 5 >>END_MODULE