FastQCFastQC Report
Thu 26 May 2016
SRR938871_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938871_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3509628
Sequences flagged as poor quality0
Sequence length101
%GC44

[WARN]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT117960.33610399734672736No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT106950.3047331512057688No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT89880.2560955178155634No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT58680.16719720722538114No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT57880.16491776336409444No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT46070.13126747336184916No Hit
GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA44940.1280477589077817No Hit
CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC39380.11220562407183897No Hit
GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA36100.10285990424056339No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTTGCGT7500.025.8946133
GTACATG101550.023.3685911
TACATGG103450.021.9784772
GAGTACT93150.021.90747812-13
CTTTGCG9000.021.5788442
ACATGGG105150.020.4068413
AGTACTT102350.019.89200412-13
GTACTTT104100.019.6263914-15
TGGACCG4550.018.7390675
TACCGTA1350.00958013817.5360227
CATGGGA54500.016.426774
TACTTTT123150.016.37893114-15
ACTTTTT127700.016.37227416-17
CATGGGG59450.015.8557964
GGACCGT3001.518214E-615.7824216
AGAGTAC197950.015.59461210-11
GTATCAA303500.015.5911771
TTGCGTT12600.015.0375224
AGTACAT79450.015.0242742
GTGTAGC18900.014.8160581
Produced by FastQC (version 0.11.5)