FastQCFastQC Report
Thu 26 May 2016
SRR938871_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938871_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3509628
Sequences flagged as poor quality0
Sequence length101
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT112070.31932159191800386No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT111980.31906515448360906No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT95560.27227956923069907No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT63180.18001907894511895No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT53000.1510131558102454No Hit
GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA42850.12209271182017012No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT41590.11850258773864353No Hit
GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA36940.10525332029491445No Hit
CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC35920.10234702937177388No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATAGCG2554.965841E-1022.3312745
GTACATG108900.020.9503731
GAGTACT106750.020.56202112-13
TACATGG110450.020.064282
AGTACTT114650.019.5177412-13
TTTGCGT7800.019.468293
ACATGGG109650.018.9988943
GTACTTT115000.018.88084414-15
GGACCGT2552.706147E-718.6093946
CTTTGCG9200.017.0215262
TACTTTT137650.016.15330714-15
GTAAGCG3250.016.06179694-95
AGAGTAC218750.015.88103310-11
GTATCAA311500.015.5658851
CATGGGG59450.015.5652174
ACTTTTT142300.015.34098316-17
TATTCCG3755.9226295E-815.1852655
CATGGGA57300.015.1554524
ATAGCGT3555.2714313E-714.7040436
AACCGTG9500.013.986437
Produced by FastQC (version 0.11.5)