##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938871_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 3509628 Sequences flagged as poor quality 0 Sequence length 101 %GC 44 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.10574226100316 33.0 31.0 34.0 30.0 34.0 2 32.36205916980375 34.0 31.0 34.0 30.0 34.0 3 32.453476835721624 34.0 31.0 34.0 30.0 34.0 4 36.01699553342976 37.0 35.0 37.0 35.0 37.0 5 35.937803094800934 37.0 35.0 37.0 35.0 37.0 6 35.973416555828706 37.0 35.0 37.0 35.0 37.0 7 35.91094982146256 37.0 35.0 37.0 35.0 37.0 8 35.8767820977038 37.0 35.0 37.0 35.0 37.0 9 37.544531785135064 39.0 37.0 39.0 35.0 39.0 10-11 37.44323244514803 39.0 37.0 39.0 34.0 39.0 12-13 37.348719009536055 39.0 37.0 39.0 34.0 39.0 14-15 38.63876769845693 40.0 38.0 41.0 34.0 41.0 16-17 38.73652307310063 40.0 38.0 41.0 34.0 41.0 18-19 38.77155356636088 40.0 38.0 41.0 34.0 41.0 20-21 38.73979094080626 40.0 38.0 41.0 34.0 41.0 22-23 38.66097731725414 40.0 38.0 41.0 34.0 41.0 24-25 38.57916123874097 40.0 38.0 41.0 34.0 41.0 26-27 38.40380932110184 40.0 38.0 41.0 34.0 41.0 28-29 38.241363187209586 40.0 38.0 41.0 33.5 41.0 30-31 38.05200750620864 40.0 37.5 41.0 33.0 41.0 32-33 37.96542525304676 40.0 37.0 41.0 33.0 41.0 34-35 37.865547003841996 40.0 37.0 41.0 33.0 41.0 36-37 37.73506608107754 40.0 37.0 41.0 33.0 41.0 38-39 37.5879014812966 40.0 37.0 41.0 32.5 41.0 40-41 37.421566046316016 40.0 37.0 41.0 32.0 41.0 42-43 37.24300723609454 40.0 36.5 41.0 31.0 41.0 44-45 36.99823599538185 40.0 36.0 41.0 31.0 41.0 46-47 36.70022535151874 39.0 36.0 41.0 30.0 41.0 48-49 36.368343311598835 39.0 35.5 41.0 30.0 41.0 50-51 36.103210226269 39.0 35.0 40.0 29.0 41.0 52-53 35.827021695746666 39.0 35.0 40.0 28.5 41.0 54-55 35.50978337305264 38.0 35.0 40.0 28.0 41.0 56-57 35.34785125375111 38.0 34.5 40.0 28.0 41.0 58-59 35.04489150986942 38.0 34.0 40.0 27.0 41.0 60-61 34.641743654883086 38.0 34.0 40.0 26.0 41.0 62-63 34.19054398357889 37.0 33.0 40.0 26.0 41.0 64-65 33.74614588782629 36.5 33.0 39.0 25.0 40.5 66-67 33.24766057827211 36.0 32.0 39.0 23.5 40.0 68-69 32.71861490733491 35.5 31.5 38.5 22.0 40.0 70-71 32.14624641130057 35.0 31.0 38.0 21.5 40.0 72-73 31.58119079856897 35.0 31.0 37.0 19.5 39.0 74-75 31.01871266698351 34.0 30.0 36.5 18.0 39.0 76-77 29.149322378326136 32.5 28.0 34.5 13.0 37.0 78-79 30.115428187830734 34.0 29.5 35.0 13.5 37.0 80-81 30.079312821757746 34.0 30.0 35.0 12.0 37.0 82-83 29.608119008624275 34.0 29.0 35.0 7.0 37.0 84-85 29.09107703152585 34.0 29.0 35.0 3.5 36.0 86-87 28.323299078990708 33.0 27.5 35.0 2.0 36.0 88-89 27.543997825410557 33.0 26.0 35.0 2.0 35.0 90-91 26.873157354568633 32.0 25.0 34.0 2.0 35.0 92-93 26.364149847220276 32.0 25.0 34.0 2.0 35.0 94-95 25.68470362101055 32.0 23.5 34.0 2.0 35.0 96-97 24.66743170501261 31.0 18.5 34.0 2.0 35.0 98-99 23.242021661555015 31.0 2.0 34.0 2.0 35.0 100-101 17.602628398223402 23.5 2.0 30.5 2.0 32.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.14452128856806468 1101 2 0.1194990183976472 1101 3 0.14677520933439325 1101 4 0.15001799652004166 1101 5 0.083362948379758 1101 6 0.14088116794885508 1101 7 0.14727128703792403 1101 8 0.0824589702270444 1101 9 0.17238449931144828 1101 10-11 0.19138454650980208 1101 12-13 0.23962711365269485 1101 14-15 0.23885851664234536 1101 16-17 0.21562015574335902 1101 18-19 0.25416361114313446 1101 20-21 0.3245737236289017 1101 22-23 0.2664996460404083 1101 24-25 0.1757154962448979 1101 26-27 0.13580316760560862 1101 28-29 0.13451387743632637 1101 30-31 0.11642779561957894 1101 32-33 0.1804934996051628 1101 34-35 0.2249215611322839 1101 36-37 0.34994401712491907 1101 38-39 0.24767606680084242 1101 40-41 0.27310928451351657 1101 42-43 0.17350805017500903 1101 44-45 0.313812940022828 1101 46-47 0.1873332114331845 1101 48-49 0.13524022979704142 1101 50-51 -0.025846516202236103 1101 52-53 -0.04773033702020513 1101 54-55 -0.059497134054254275 1101 56-57 0.04469738116516453 1101 58-59 0.10592848740096628 1101 60-61 0.08701688152972054 1101 62-63 0.1750057694420093 1101 64-65 0.10998389755980043 1101 66-67 -0.036753086265150614 1101 68-69 -0.008520637851113122 1101 70-71 0.053190202523829555 1101 72-73 0.14318818571563696 1101 74-75 0.23529657057228448 1101 76-77 0.08244818332767778 1101 78-79 0.39352540912683054 1101 80-81 0.5050802672898804 1101 82-83 0.3511240097414188 1101 84-85 0.5900121719984277 1101 86-87 0.5917141617549753 1101 88-89 0.6310339601537898 1101 90-91 0.592598388285122 1101 92-93 0.5149126339850092 1101 94-95 0.5608735405019623 1101 96-97 0.7141864570392933 1101 98-99 1.0348143464721247 1101 100-101 1.0466440384953728 1102 1 0.1597480362506758 1102 2 0.1291659509873142 1102 3 0.09887966181690899 1102 4 0.07662461969223955 1102 5 0.08881216264352076 1102 6 0.0651911114748529 1102 7 0.1307362347491221 1102 8 0.1567748730462668 1102 9 0.1719648372056568 1102 10-11 0.18727902729096968 1102 12-13 0.1822078392434534 1102 14-15 0.23415177095073858 1102 16-17 0.28477161175167254 1102 18-19 0.22071192913832505 1102 20-21 0.20695038110013542 1102 22-23 0.24485161148837875 1102 24-25 0.26968437183814586 1102 26-27 0.28737493690379523 1102 28-29 0.2602909487245455 1102 30-31 0.23730409028173938 1102 32-33 0.1914367712434455 1102 34-35 0.22775622111236515 1102 36-37 0.24755189745682316 1102 38-39 0.22964128188110067 1102 40-41 0.3680550732111314 1102 42-43 0.359574035305549 1102 44-45 0.3083704385261541 1102 46-47 0.49785837357847385 1102 48-49 0.39632170680672374 1102 50-51 0.36042907165578697 1102 52-53 0.28722400018082084 1102 54-55 0.33724534730411904 1102 56-57 0.33767381752608117 1102 58-59 0.3394201720178103 1102 60-61 0.2912371571002197 1102 62-63 0.35535357297437997 1102 64-65 0.30497466636342097 1102 66-67 0.10149721957673563 1102 68-69 0.07311976149917143 1102 70-71 0.23791801808439317 1102 72-73 0.34402317174440356 1102 74-75 0.37523785440953716 1102 76-77 0.1636060854190049 1102 78-79 0.38537126938162913 1102 80-81 0.29597378225455273 1102 82-83 0.26911637179976466 1102 84-85 0.5907224923526826 1102 86-87 0.7750700612497852 1102 88-89 0.5486650657465297 1102 90-91 0.6313151629420979 1102 92-93 0.6627588632136714 1102 94-95 0.7708056045591114 1102 96-97 0.952959171422016 1102 98-99 1.2347333822813127 1102 100-101 1.299278205931877 1103 1 0.11883836691092853 1103 2 0.09818879658179469 1103 3 0.12235288252160359 1103 4 0.08137928656426396 1103 5 0.09202884943610457 1103 6 0.041854521221104335 1103 7 0.11951433251462618 1103 8 0.10374569129427158 1103 9 0.13154598158445197 1103 10-11 0.17228972925107655 1103 12-13 0.10332896810043479 1103 14-15 0.18838161668485043 1103 16-17 0.13495181648433885 1103 18-19 0.20396527767669426 1103 20-21 0.19468017467620058 1103 22-23 0.18819180092135213 1103 24-25 0.1814501379089748 1103 26-27 0.08325696247161574 1103 28-29 0.16602076196227955 1103 30-31 0.2057825531900761 1103 32-33 0.14470369544167028 1103 34-35 0.08783439630482803 1103 36-37 0.02762702690986174 1103 38-39 0.06894015921754004 1103 40-41 0.23488931016238013 1103 42-43 0.14604467140996036 1103 44-45 0.19183299277098342 1103 46-47 0.1576698963771932 1103 48-49 0.2316213899078221 1103 50-51 0.20414691093213833 1103 52-53 0.2163899409128689 1103 54-55 0.4236679761468167 1103 56-57 0.4440650641474093 1103 58-59 0.3152429540395403 1103 60-61 0.3399213812804831 1103 62-63 0.4543761054384561 1103 64-65 0.41211851829351076 1103 66-67 0.29201563974640976 1103 68-69 0.30374927742686 1103 70-71 0.3110207254063013 1103 72-73 0.2692319723839205 1103 74-75 0.2773010834504177 1103 76-77 0.09882063163750843 1103 78-79 0.48580288870825683 1103 80-81 0.5198049092018096 1103 82-83 0.5232933858791604 1103 84-85 0.6815390964205754 1103 86-87 0.7359995324471562 1103 88-89 0.610029615269287 1103 90-91 0.7377480540879553 1103 92-93 0.9665776069453749 1103 94-95 1.168920022412351 1103 96-97 0.9742478068451881 1103 98-99 1.253325500606742 1103 100-101 1.1342232961471197 1104 1 0.10113946344743852 1104 2 0.06491758944977732 1104 3 0.06845976132298404 1104 4 0.08119979074646011 1104 5 -0.02215621057092676 1104 6 0.07332008374397248 1104 7 0.06310138708063562 1104 8 0.07902471582234938 1104 9 0.07484649489539663 1104 10-11 0.11454088936279305 1104 12-13 0.12211009912332571 1104 14-15 0.176162588316906 1104 16-17 0.13650940910309828 1104 18-19 0.15884499193239066 1104 20-21 0.20457472314731007 1104 22-23 0.14973704075023164 1104 24-25 0.08740963452372341 1104 26-27 0.04929530321300746 1104 28-29 0.08204461262755558 1104 30-31 0.11341492909163264 1104 32-33 0.19832286363885743 1104 34-35 0.21730159620629053 1104 36-37 0.2626931016569145 1104 38-39 0.24723945898461608 1104 40-41 0.283547891366581 1104 42-43 0.2400914236405285 1104 44-45 0.2356976228574581 1104 46-47 0.23054193813759127 1104 48-49 0.10724962527012849 1104 50-51 0.13263530619242658 1104 52-53 0.06527112591130191 1104 54-55 0.33972642186880364 1104 56-57 0.2635033201218562 1104 58-59 0.3888003763998924 1104 60-61 0.32358744298647224 1104 62-63 0.31422528185875365 1104 64-65 0.3248197039439944 1104 66-67 0.32713976536088296 1104 68-69 0.36714336040427753 1104 70-71 0.3140624640705383 1104 72-73 0.34380152656050456 1104 74-75 0.31409383189182805 1104 76-77 0.253929651347633 1104 78-79 0.42593206773677394 1104 80-81 0.5000199781564589 1104 82-83 0.3955208003786588 1104 84-85 0.5190938417393731 1104 86-87 0.536946458920891 1104 88-89 0.5485539278057381 1104 90-91 0.6659357802689847 1104 92-93 0.969517915076846 1104 94-95 1.17764205203731 1104 96-97 1.0235040131259083 1104 98-99 1.115582022046624 1104 100-101 0.817443335197872 1105 1 -0.018575281130900123 1105 2 0.031537280795326694 1105 3 0.0298841594184438 1105 4 0.05099962269889602 1105 5 0.01952490322280198 1105 6 0.059345191609949666 1105 7 0.13638661059452772 1105 8 0.06701232851879979 1105 9 0.045843577107525846 1105 10-11 0.12069775857286658 1105 12-13 0.13977140174011993 1105 14-15 0.027039785964241503 1105 16-17 0.09804840925554714 1105 18-19 0.13399238808148084 1105 20-21 0.09677540171912113 1105 22-23 0.07411803010366924 1105 24-25 -0.058875326637434 1105 26-27 -0.09226544739944842 1105 28-29 0.009518616662113288 1105 30-31 0.08037275411653155 1105 32-33 0.08465543827643529 1105 34-35 0.09610828123765458 1105 36-37 0.05993547304202451 1105 38-39 0.08961385761187302 1105 40-41 0.2378010192138973 1105 42-43 0.26759556278674523 1105 44-45 0.08426469829805683 1105 46-47 0.23397042751762598 1105 48-49 0.1216979931879365 1105 50-51 0.15144333844054358 1105 52-53 0.13594682602084873 1105 54-55 0.23064334468391223 1105 56-57 0.30420256107156973 1105 58-59 0.20002588275840338 1105 60-61 0.1760149148633232 1105 62-63 0.2138711942967575 1105 64-65 0.09244085595612717 1105 66-67 0.22181790464182427 1105 68-69 0.2856216790777637 1105 70-71 0.40022663661355296 1105 72-73 0.31485143385071623 1105 74-75 0.22592992879216567 1105 76-77 0.026593914295983012 1105 78-79 0.11888080613918461 1105 80-81 0.14111859796834736 1105 82-83 0.2406883693260191 1105 84-85 0.2925877952178517 1105 86-87 0.44645922734496324 1105 88-89 0.4088866371649935 1105 90-91 0.2854580146634298 1105 92-93 0.5860538485385156 1105 94-95 0.7086274170089659 1105 96-97 0.6287086128075394 1105 98-99 0.9218245104268767 1105 100-101 0.8787010152197112 1106 1 -0.03517638652800059 1106 2 0.02500491915337477 1106 3 -0.02676253405136464 1106 4 -0.055611577790465105 1106 5 0.02880887496480966 1106 6 0.02367138811603553 1106 7 0.10075052162132181 1106 8 0.036752366977282236 1106 9 -0.022251281628349773 1106 10-11 0.04895957380890792 1106 12-13 0.03069119173739665 1106 14-15 -0.05445044390834397 1106 16-17 -0.14271721618036537 1106 18-19 -0.07943847603568344 1106 20-21 -0.07480526034120771 1106 22-23 -0.08559883200687324 1106 24-25 0.02663563278897385 1106 26-27 -0.04084918965118334 1106 28-29 0.029686327452701278 1106 30-31 0.10647688545812883 1106 32-33 0.07209495296805102 1106 34-35 -0.03838040802259712 1106 36-37 0.015581130137476862 1106 38-39 0.06233230993193217 1106 40-41 0.2098114201554253 1106 42-43 0.14432797671239683 1106 44-45 0.19587978906605485 1106 46-47 0.25194346318522776 1106 48-49 0.31914642503883073 1106 50-51 0.2752464190452599 1106 52-53 0.36511471970103315 1106 54-55 0.3120764861381673 1106 56-57 0.34618786377451016 1106 58-59 0.12219292812019233 1106 60-61 0.2018596942953721 1106 62-63 0.2914334310054869 1106 64-65 0.2964707299896361 1106 66-67 0.36604572307895467 1106 68-69 0.33069879752306264 1106 70-71 0.374318827434557 1106 72-73 0.37475010061658054 1106 74-75 0.2731877731541523 1106 76-77 0.15826408892971244 1106 78-79 0.4275281774370896 1106 80-81 0.40424712595510215 1106 82-83 0.42936585024402163 1106 84-85 0.4578551498387071 1106 86-87 0.7386300028278399 1106 88-89 0.9692466610129706 1106 90-91 0.8634658510175406 1106 92-93 0.866034567635598 1106 94-95 0.9415081244300474 1106 96-97 1.1138131853573618 1106 98-99 1.1355043937081852 1106 100-101 1.3574297672203883 1107 1 0.026444828292042644 1107 2 0.06123606038809726 1107 3 0.0998907928336763 1107 4 0.1249732209143275 1107 5 0.015148708523710752 1107 6 0.04722229386284482 1107 7 0.10423322633901932 1107 8 0.028924422080081058 1107 9 0.062211137520584714 1107 10-11 0.07381984526315222 1107 12-13 0.06532590653148418 1107 14-15 0.1407794000554503 1107 16-17 0.15675546830774323 1107 18-19 0.13219053695748784 1107 20-21 0.13784812980883743 1107 22-23 0.1349327006081822 1107 24-25 0.15846831681021456 1107 26-27 0.10021257306410547 1107 28-29 0.06347825595445755 1107 30-31 0.15222264370022032 1107 32-33 0.17399749576454582 1107 34-35 0.19152749741347463 1107 36-37 0.28171552496963415 1107 38-39 0.35611850229374653 1107 40-41 0.24015430633541968 1107 42-43 0.2611041814920796 1107 44-45 0.21616929074587432 1107 46-47 0.34805285988235113 1107 48-49 0.31675707155812916 1107 50-51 0.1493653819525136 1107 52-53 0.1873844160509961 1107 54-55 0.23288476932044944 1107 56-57 0.27677004053389 1107 58-59 0.2321962250051044 1107 60-61 0.37069416005538613 1107 62-63 0.44001831631905475 1107 64-65 0.35039247523769745 1107 66-67 0.28182405772410846 1107 68-69 0.3565554928129089 1107 70-71 0.49643031052359987 1107 72-73 0.541808536675191 1107 74-75 0.4628293210762955 1107 76-77 0.30980902374561126 1107 78-79 0.5217203399855279 1107 80-81 0.4552738205733995 1107 82-83 0.30445721658376 1107 84-85 0.5447736974974724 1107 86-87 0.6009153225893087 1107 88-89 0.8536432693320464 1107 90-91 0.7732410301936774 1107 92-93 0.8977917396289605 1107 94-95 1.0406123748403822 1107 96-97 1.2426790535655279 1107 98-99 1.3706226967309831 1107 100-101 1.148360491870278 1108 1 0.02179554103124559 1108 2 0.0748447198873734 1108 3 0.0707822989659661 1108 4 0.0766212790030707 1108 5 0.014592793670814785 1108 6 0.09654438222172956 1108 7 0.07627189574826332 1108 8 0.1572539459309894 1108 9 0.149078678592808 1108 10-11 0.13914267588875617 1108 12-13 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-0.8624377285613463 2316 58-59 -0.9797582353897738 2316 60-61 -0.824424339045926 2316 62-63 -0.8849397900592635 2316 64-65 -0.861271809641508 2316 66-67 -0.7809752270401731 2316 68-69 -0.5630500083809764 2316 70-71 -0.6380218062694922 2316 72-73 -0.5720148524634823 2316 74-75 -0.7366914164627154 2316 76-77 -0.6007718440415601 2316 78-79 -0.8159733702826237 2316 80-81 -1.014066429293738 2316 82-83 -0.8811798483974265 2316 84-85 -1.1739329911927001 2316 86-87 -1.2888858234951321 2316 88-89 -1.3384854691000108 2316 90-91 -1.508426393762921 2316 92-93 -1.5553170247636494 2316 94-95 -1.6493821763836074 2316 96-97 -1.4886800870096089 2316 98-99 -1.4852141029346377 2316 100-101 -0.6249353095744397 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 2.0 3 6.0 4 30.0 5 60.0 6 151.0 7 431.0 8 1055.0 9 1939.0 10 3409.0 11 5146.0 12 7257.0 13 9884.0 14 12287.0 15 16664.0 16 21512.0 17 29323.0 18 29852.0 19 23235.0 20 23004.0 21 25271.0 22 27677.0 23 30817.0 24 35578.0 25 41793.0 26 49732.0 27 59143.0 28 71586.0 29 86459.0 30 103741.0 31 126014.0 32 153725.0 33 194297.0 34 259148.0 35 365510.0 36 549580.0 37 735113.0 38 393816.0 39 15381.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 41.36015573337238 20.312811049152344 15.449546826411979 22.877486391063293 2 15.316609053723074 21.452530011727738 39.68702665923568 23.543834275313507 3 19.25790995512915 24.96885709824517 30.48664986716541 25.28658307946027 4 13.239608300366875 17.675577012720435 37.224628935032435 31.860185751880255 5 13.559328794960607 37.60230428979938 34.6358645417691 14.20250237347092 6 31.18085449512028 36.9131429313876 17.380047116104613 14.525955457387507 7 27.51573671055736 33.11134399429228 21.397395963332865 17.975523331817502 8 25.14377592155066 35.74025509256252 20.04947532900923 19.066493656877594 9 25.479623481462994 17.64397822219335 21.137254432663518 35.73914386368014 10-11 24.528083888092983 26.83519449924607 27.59249698258619 21.044224630074755 12-13 25.60992219118379 24.255590621000287 28.04255892647312 22.091928261342797 14-15 22.54324999230681 25.744159726646597 26.629703247895197 25.082887033151398 16-17 21.62670006520808 28.867104739959103 27.311699030170526 22.194496164662294 18-19 21.56339196991121 28.04645812037654 29.06231101011864 21.327838899593615 20-21 22.495788727466273 26.875170245963393 28.864255698894585 21.764785327675753 22-23 22.086728280034237 26.770999661502586 28.87593784868368 22.266334209779497 24-25 22.270049441193702 26.97518309653684 28.89063044930169 21.86413701296777 26-27 22.146758351636564 26.835476652572577 29.2249616875186 21.792803308272262 28-29 21.7852860759032 27.302352272092655 29.11867012686245 21.793691525141696 30-31 22.082226378408194 26.981506302092416 28.876878119276462 22.059389200222927 32-33 21.95148602643927 27.093056586054136 28.871592658823104 22.083864728683498 34-35 22.442472670983303 26.970310217342643 28.74513430868249 21.842082802991563 36-37 22.341297656872655 26.79680351665038 29.009985911111613 21.851912915365354 38-39 22.113032300275815 27.08699095270839 29.049274954284805 21.750701792730993 40-41 22.138410819739725 27.091407777482225 28.89144456439212 21.87873683838593 42-43 21.88558664604795 27.240796447745264 28.489032273874443 22.384584632332345 44-45 22.16145020821669 27.058168177537674 28.446417148563995 22.333964465681643 46-47 22.410380035123826 27.298196495396105 28.130132820603926 22.161290648876143 48-49 22.437348251996923 27.244289032200708 28.102255608247166 22.216107107555196 50-51 22.1358370163235 27.488792891959573 28.302916312756743 22.072453778960185 52-53 22.38760062319995 27.648813492484102 27.786434915609288 22.177150968706655 54-55 22.101943567808327 27.337569679749535 27.991057741732174 22.569429010709968 56-57 22.36385166747017 27.57558635844027 27.722382543107134 22.33817943098243 58-59 22.540126763860176 27.276597181470862 27.880527006452166 22.302749048216796 60-61 22.339705767331292 27.30275038006798 28.245786270937216 22.111757581663504 62-63 22.213804002668695 27.305733002220915 28.267642015071022 22.212820980039368 64-65 22.13585115220445 27.505112051991134 28.074749860988373 22.28428693481605 66-67 22.124564293385237 27.442799304408084 27.75953696350707 22.67309943869961 68-69 21.896599647787944 27.42689306142825 28.048799078282055 22.627708212501744 70-71 22.256250841435453 27.19547872245701 27.85191590845467 22.69635452765286 72-73 22.171480852101705 26.992347906957658 28.00655795998892 22.829613280951712 74-75 21.989623971543423 27.683560764844593 27.57406198035803 22.752753283253952 76-77 22.16316061449168 27.13286266564913 27.78939902603958 22.914577693819606 78-79 22.023258471179936 27.138549264756374 27.97279511682092 22.86539714724277 80-81 22.109770920937834 27.301817462755206 27.7425349425493 22.845876673757655 82-83 22.06032947081571 27.170073295517362 27.944756538299785 22.824840695367143 84-85 21.974104333743064 27.17784137437023 27.796440756297976 23.051613535588732 86-87 21.887256226528468 27.194444274126244 27.94590402390298 22.97239547544231 88-89 21.945136193784816 27.07611360198102 27.67562492792787 23.30312527630629 90-91 21.932286336101242 26.81888038516853 27.890297669617713 23.358535609112515 92-93 21.914231365831363 27.096376026177133 27.797504464860662 23.19188814313084 94-95 21.7993225995151 26.960604833665897 27.79652797347157 23.443544593347436 96-97 21.51624659828523 26.875861736874036 27.96170362263568 23.646188042205054 98-99 21.576004636388813 26.98680600906991 27.55688067225358 23.880308682287694 100-101 21.829231981285695 25.93579352721025 28.515862851053463 23.719111640450592 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 1280.0 1 902.5 2 589.0 3 660.0 4 569.0 5 594.5 6 1037.0 7 2099.0 8 3344.0 9 3883.0 10 3487.0 11 3067.5 12 3114.5 13 3266.5 14 3503.5 15 3716.5 16 3973.5 17 4314.5 18 4676.5 19 5161.5 20 5742.0 21 6742.0 22 8326.0 23 10217.0 24 12523.0 25 15440.0 26 19902.0 27 24998.0 28 29498.5 29 34620.5 30 40727.5 31 47660.5 32 54750.5 33 61669.0 34 69829.0 35 77378.5 36 85995.0 37 94406.5 38 98586.5 39 101463.5 40 103882.0 41 106516.0 42 109139.0 43 113358.5 44 120372.0 45 125434.5 46 128343.5 47 138880.0 48 164244.5 49 164189.0 50 139973.0 51 128798.0 52 123022.5 53 119450.5 54 114847.5 55 107139.5 56 97232.0 57 86775.0 58 77707.0 59 67975.5 60 56311.5 61 44067.0 62 33935.0 63 26081.0 64 19261.0 65 13525.0 66 8977.5 67 6290.5 68 4282.0 69 2645.0 70 1647.0 71 941.0 72 541.5 73 325.5 74 185.5 75 107.0 76 60.5 77 28.0 78 16.0 79 13.5 80 9.5 81 7.5 82 6.0 83 3.5 84 2.0 85 0.5 86 1.0 87 1.0 88 1.0 89 0.5 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.3816928745724618 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 9.117775445146892E-4 16-17 0.002664100012878858 18-19 4.416422481243026E-4 20-21 0.0 22-23 0.0 24-25 1.1397219306433615E-4 26-27 4.273957239912606E-5 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.014004333222780305 36-37 0.023777448778047133 38-39 0.0114257123546997 40-41 0.00648216848053412 42-43 0.0055561444118863886 44-45 0.0017950620407632945 46-47 0.0012679406478407398 48-49 0.0031057422610031604 50-51 7.12326206652101E-4 52-53 0.0 54-55 0.0 56-57 0.0 58-59 8.975310203816472E-4 60-61 0.0016668433235659163 62-63 0.0012679406478407398 64-65 8.405449238494793E-4 66-67 0.00282081177834232 68-69 0.002692593061144942 70-71 1.4246524133042019E-5 72-73 0.0 74-75 0.0 76-77 0.08889831059018222 78-79 0.0031627283575353282 80-81 2.849304826608404E-4 82-83 0.0 84-85 1.7095828959650425E-4 86-87 0.08888406406604916 88-89 0.42354916247533925 90-91 0.375666594864185 92-93 0.0 94-95 9.972566893129413E-4 96-97 2.8493048266084038E-5 98-99 0.0 100-101 3.7040962745909253E-4 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 3509628.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 48.705068810374094 #Duplication Level Percentage of deduplicated Percentage of total 1 70.81846147409202 34.49218039140479 2 15.008406914793454 14.619709830382194 3 5.702502219570719 8.332222889865088 4 2.7827814510717657 5.421422482347322 5 1.501679204049372 3.656969448216624 6 0.9672871961420311 2.826707366849487 7 0.6503961320609973 2.2174311856222424 8 0.4326531826516259 1.6857922425659835 9 0.3275580596542295 1.4358364051366683 >10 1.651345096465484 14.15646751787913 >50 0.10492829543171192 3.487055187725465 >100 0.04814014195889245 4.077613375107866 >500 0.0019303160329873774 0.6522443373011372 >1k 0.0016378438996464155 1.6967925269463044 >5k 1.7548327496211594E-4 0.6032419652365937 >10k+ 1.1698884997474398E-4 0.6383128474131426 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 11207 0.31932159191800386 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 11198 0.31906515448360906 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 9556 0.27227956923069907 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6318 0.18001907894511895 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 5300 0.1510131558102454 No Hit GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 4285 0.12209271182017012 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 4159 0.11850258773864353 No Hit GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA 3694 0.10525332029491445 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 3592 0.10234702937177388 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 2.849304826608404E-5 0.0 0.0 0.0 0.0 8 2.849304826608404E-5 0.0 0.0 0.0 0.0 9 2.849304826608404E-5 0.0 0.0 0.0 0.0 10-11 2.849304826608404E-5 0.0 0.0 5.698609653216808E-5 0.0 12-13 2.849304826608404E-5 0.0 0.0 7.12326206652101E-5 0.0 14-15 2.849304826608404E-5 0.0 0.0 8.547914479825212E-5 0.0 16-17 2.849304826608404E-5 0.0 0.0 9.972566893129414E-5 0.0 18-19 5.698609653216808E-5 0.0 0.0 1.2821871719737818E-4 0.0 20-21 8.547914479825212E-5 0.0 0.0 1.5671176546346223E-4 0.0 22-23 1.1397219306433616E-4 0.0 0.0 2.849304826608404E-4 0.0 24-25 1.7095828959650425E-4 0.0 0.0 4.986283446564708E-4 0.0 26-27 1.7095828959650425E-4 0.0 0.0 8.832844962486052E-4 0.0 28-29 1.994513378625883E-4 0.0 0.0 0.0026641000128788577 0.0 30-31 1.994513378625883E-4 0.0 0.0 0.007408192549181851 0.0 32-33 2.136978619956303E-4 0.0 0.0 0.017238294200980844 0.0 34-35 2.4219091026171435E-4 0.0 0.0 0.03226837716134018 0.0 36-37 2.849304826608404E-4 0.0 0.0 0.05653020775991073 0.0 38-39 3.561631033260505E-4 0.0 0.0 0.09191857370638712 0.0 40-41 3.8465615159213455E-4 0.0 0.0 0.12994254661747628 0.0 42-43 4.131491998582186E-4 0.0 0.0 0.1714854109894268 0.0 44-45 4.273957239912606E-4 0.0 0.0 0.22627754280510642 0.0 46-47 4.273957239912606E-4 0.0 0.0 0.2924241543548205 0.0 48-49 4.273957239912606E-4 0.0 0.0 0.3641554033646871 0.0 50-51 4.273957239912606E-4 0.0 0.0 0.44107238715898095 0.0 52-53 4.273957239912606E-4 0.0 0.0 0.5226622308689126 0.0 54-55 4.273957239912606E-4 0.0 0.0 0.6135835478859868 0.0 56-57 4.273957239912606E-4 0.0 0.0 0.7265157446886109 0.0 58-59 4.273957239912606E-4 0.0 0.0 0.8544780244515943 0.0 60-61 4.273957239912606E-4 0.0 0.0 0.9978977828989284 0.0 62-63 4.273957239912606E-4 1.424652413304202E-5 0.0 1.148312584695586 0.0 64-65 4.273957239912606E-4 2.849304826608404E-5 0.0 1.308984883868034 0.0 66-67 4.843818205234287E-4 2.849304826608404E-5 0.0 1.4972811933344503 0.0 68-69 4.843818205234287E-4 2.849304826608404E-5 0.0 1.707759340876013 0.0 70-71 4.843818205234287E-4 2.849304826608404E-5 0.0 1.9402626147272588 0.0 72-73 4.843818205234287E-4 2.849304826608404E-5 0.0 2.1839636565470757 0.0 74-75 4.843818205234287E-4 2.849304826608404E-5 0.0 2.44703997118783 0.0 76-77 4.843818205234287E-4 2.849304826608404E-5 0.0 2.7302038848561727 0.0 78-79 4.843818205234287E-4 2.849304826608404E-5 0.0 3.0616207757631293 0.0 80-81 4.843818205234287E-4 2.849304826608404E-5 0.0 3.4228841347288093 0.0 82-83 4.843818205234287E-4 2.849304826608404E-5 0.0 3.789333228478916 0.0 84-85 5.128748687895127E-4 2.849304826608404E-5 0.0 4.182665513268073 0.0 86-87 5.698609653216808E-4 2.849304826608404E-5 0.0 4.602111676793096 0.0 88-89 5.983540135877649E-4 2.849304826608404E-5 0.0 5.057017438885261 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TATAGCG 255 4.965841E-10 22.331274 5 GTACATG 10890 0.0 20.950373 1 GAGTACT 10675 0.0 20.562021 12-13 TACATGG 11045 0.0 20.06428 2 AGTACTT 11465 0.0 19.51774 12-13 TTTGCGT 780 0.0 19.46829 3 ACATGGG 10965 0.0 18.998894 3 GTACTTT 11500 0.0 18.880844 14-15 GGACCGT 255 2.706147E-7 18.609394 6 CTTTGCG 920 0.0 17.021526 2 TACTTTT 13765 0.0 16.153307 14-15 GTAAGCG 325 0.0 16.061796 94-95 AGAGTAC 21875 0.0 15.881033 10-11 GTATCAA 31150 0.0 15.565885 1 CATGGGG 5945 0.0 15.565217 4 ACTTTTT 14230 0.0 15.340983 16-17 TATTCCG 375 5.9226295E-8 15.185265 5 CATGGGA 5730 0.0 15.155452 4 ATAGCGT 355 5.2714313E-7 14.704043 6 AACCGTG 950 0.0 13.98643 7 >>END_MODULE