FastQCFastQC Report
Thu 26 May 2016
SRR938870_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938870_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3510389
Sequences flagged as poor quality0
Sequence length101
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT119540.3405320606918492No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT105850.3015335337479692No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT90210.2569800668814767No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT58620.16699004013515312No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT58140.1656226703080485No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT45320.12910250117579564No Hit
GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA44790.12759269699170092No Hit
CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC38700.11024419231031092No Hit
GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA36270.10332188256059371No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACATG109450.023.3945331
TACATGG111550.022.0224572
GTATACG1302.9780736E-421.8848881
GAGTACT97050.021.31360612-13
ACATGGG111750.020.6276323
AGTACTT105550.019.93354812-13
GTACTTT105500.019.51718314-15
GTGTAGC17100.018.0241031
TTTGCGT7100.018.0000363
CTTTGCG7750.017.101112
CATGGGG63600.017.043014
GTATCAA310000.016.8101831
TACTTTT123200.016.80922514-15
ACTTTTT126400.016.5904716-17
TATACCG2104.1144912E-415.7778095
CATGGGA57800.015.7232154
AGAGTAC210550.015.59024710-11
CAACGCA338300.015.2787775
TCAACGC340200.015.1656184
TATCAAC341900.015.145592
Produced by FastQC (version 0.11.5)