##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938870_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 3510389 Sequences flagged as poor quality 0 Sequence length 101 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.969706491217924 33.0 31.0 34.0 30.0 34.0 2 32.06674075152355 33.0 31.0 34.0 30.0 34.0 3 32.118070105620774 34.0 31.0 34.0 30.0 34.0 4 35.64559939083674 37.0 35.0 37.0 33.0 37.0 5 35.67561315854169 37.0 35.0 37.0 33.0 37.0 6 35.74823274571565 37.0 35.0 37.0 35.0 37.0 7 35.668878862143195 37.0 35.0 37.0 33.0 37.0 8 35.65167848919308 37.0 35.0 37.0 33.0 37.0 9 37.297399234101974 39.0 37.0 39.0 34.0 39.0 10-11 37.19579254606826 39.0 37.0 39.0 34.0 39.0 12-13 37.08441115785173 39.0 37.0 39.0 33.0 39.0 14-15 38.34229995593081 40.0 38.0 41.0 33.5 41.0 16-17 38.27377621112646 40.0 38.0 41.0 33.0 41.0 18-19 38.20742402052878 40.0 38.0 41.0 33.5 41.0 20-21 38.08485484087376 40.0 38.0 41.0 33.0 41.0 22-23 38.17310716846481 40.0 38.0 41.0 33.0 41.0 24-25 38.11854469689826 40.0 38.0 41.0 33.0 41.0 26-27 38.04261692934885 40.0 38.0 41.0 33.0 41.0 28-29 37.919438415514634 40.0 38.0 41.0 33.0 41.0 30-31 37.76376150905213 40.0 37.5 41.0 33.0 41.0 32-33 37.59670224581948 40.0 37.0 41.0 32.5 41.0 34-35 37.30196596445579 40.0 37.0 41.0 31.5 41.0 36-37 37.054147560284626 40.0 37.0 41.0 31.0 41.0 38-39 36.86234317621209 40.0 36.0 41.0 30.5 41.0 40-41 36.73305038273536 39.5 36.0 41.0 30.0 41.0 42-43 36.54875613500384 39.0 36.0 41.0 30.0 41.0 44-45 36.51084110621359 39.5 36.0 41.0 30.0 41.0 46-47 36.31852937096145 40.0 36.0 41.0 30.0 41.0 48-49 36.16175401073784 39.5 36.0 41.0 29.0 41.0 50-51 35.016124566251776 38.0 34.5 40.0 27.0 40.5 52-53 35.17451684129594 38.5 34.5 39.5 27.5 40.5 54-55 35.681552671228175 39.0 35.0 40.5 28.0 41.0 56-57 35.585391818399614 39.0 35.0 41.0 27.5 41.0 58-59 35.393867745141634 39.0 35.0 41.0 27.0 41.0 60-61 35.126996466773335 38.5 35.0 40.0 26.5 41.0 62-63 34.78177717626166 38.0 34.0 40.0 26.0 41.0 64-65 34.3808013869688 37.5 34.0 40.0 26.0 41.0 66-67 33.993352018821845 37.0 34.0 39.5 25.0 41.0 68-69 33.599366195598265 36.0 33.5 39.0 24.5 41.0 70-71 33.17439463261764 36.0 33.0 39.0 23.5 40.5 72-73 32.72684295102337 35.0 32.5 38.5 22.0 40.0 74-75 32.26103303650963 35.0 32.0 37.0 21.5 39.5 76-77 31.84167922700305 35.0 32.0 37.0 20.0 39.0 78-79 31.405197686068412 35.0 31.0 36.0 19.0 39.0 80-81 30.96635486836359 35.0 31.0 36.0 17.5 37.5 82-83 30.578896811720867 34.5 31.0 35.5 12.5 37.0 84-85 30.227622066956112 34.0 31.0 35.0 7.5 37.0 86-87 29.842254234502214 34.0 30.0 35.0 4.5 36.0 88-89 29.43129180270335 34.0 30.0 35.0 2.0 36.0 90-91 29.012120166739358 34.0 29.0 35.0 2.0 36.0 92-93 28.60108950888349 34.0 29.0 35.0 2.0 35.0 94-95 28.135076340542316 34.0 29.0 35.0 2.0 35.0 96-97 27.567201811537124 34.0 27.5 35.0 2.0 35.0 98-99 26.873372723079978 33.5 26.0 35.0 2.0 35.0 100-101 24.229540942613482 31.0 13.5 34.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.24796352078388395 1101 2 0.27542325414597 1101 3 0.25511783597006144 1101 4 0.13934432830723154 1101 5 0.10362681827623277 1101 6 0.1642156756469717 1101 7 0.22875515811323766 1101 8 0.22883564083508645 1101 9 0.19527174904666822 1101 10-11 0.24722361489811107 1101 12-13 0.3442471275743131 1101 14-15 0.16745017230357462 1101 16-17 0.2907312388955887 1101 18-19 0.31765220764295066 1101 20-21 0.41896310303720696 1101 22-23 0.3063048423970045 1101 24-25 0.39054418307712524 1101 26-27 0.3220976776822795 1101 28-29 0.2985691173915157 1101 30-31 0.31708590991552654 1101 32-33 0.33227884625533477 1101 34-35 0.24123887394618748 1101 36-37 0.18103768789256947 1101 38-39 0.2168116082057594 1101 40-41 0.17999061285309637 1101 42-43 0.0899205441666524 1101 44-45 0.19466459826200833 1101 46-47 0.26618680474437895 1101 48-49 0.4295037905837802 1101 50-51 0.3961552133776891 1101 52-53 0.34960726393417474 1101 54-55 0.29962129237311075 1101 56-57 0.2684606464434083 1101 58-59 0.12850319746307548 1101 60-61 0.14251868725300199 1101 62-63 0.14670109533026476 1101 64-65 0.14494000083367808 1101 66-67 0.02459387038891947 1101 68-69 0.09644172289730335 1101 70-71 0.10303755441669438 1101 72-73 -0.08162857905017518 1101 74-75 -0.14543127193119432 1101 76-77 -0.12389048723487406 1101 78-79 -0.0738250203255113 1101 80-81 -0.30938148373630625 1101 82-83 -0.26962452615812893 1101 84-85 -0.3728929655077202 1101 86-87 -0.3955076285774659 1101 88-89 -0.4297925120500139 1101 90-91 -0.4058525952189349 1101 92-93 -0.2832196980714805 1101 94-95 -0.34451146013679335 1101 96-97 -0.5024071716862544 1101 98-99 -0.5592643661182422 1101 100-101 -0.2727182223418687 1102 1 0.1629317958116232 1102 2 0.08152857690888027 1102 3 0.04963273351682318 1102 4 0.15385758542195305 1102 5 0.08410154947545578 1102 6 0.24428860637542726 1102 7 0.2676800287843122 1102 8 0.13245217877877025 1102 9 0.3108933164777383 1102 10-11 0.17620423566990695 1102 12-13 0.3160459666964144 1102 14-15 0.37008164309668956 1102 16-17 0.27845175318332593 1102 18-19 0.2642742787955399 1102 20-21 0.3977432626614288 1102 22-23 0.3279076112495858 1102 24-25 0.38323389798970453 1102 26-27 0.3536447754699452 1102 28-29 0.3541644835749409 1102 30-31 0.3437285286211562 1102 32-33 0.2866004099278072 1102 34-35 0.23951009743098695 1102 36-37 0.3013336963033808 1102 38-39 0.27765947446733463 1102 40-41 0.21710780292999488 1102 42-43 0.10065637278329831 1102 44-45 0.25593631598405153 1102 46-47 0.11072214621945164 1102 48-49 0.13560857765956058 1102 50-51 -0.026004397850449834 1102 52-53 0.12702373099152453 1102 54-55 0.06896491054796883 1102 56-57 0.06895284631196574 1102 58-59 0.11884611977158244 1102 60-61 0.016574648467148734 1102 62-63 -0.04300521112983802 1102 64-65 -0.04668639269929997 1102 66-67 -0.01097465699136535 1102 68-69 -0.11469026830268092 1102 70-71 -0.18742618729288552 1102 72-73 -0.2521195687927289 1102 74-75 -0.11363266464746147 1102 76-77 -0.09430852692911529 1102 78-79 -0.16877692440344205 1102 80-81 -0.24254063971820727 1102 82-83 -0.3912016171557404 1102 84-85 -0.38437678020423505 1102 86-87 -0.2755009790317118 1102 88-89 -0.37219986715718534 1102 90-91 -0.3225118413016581 1102 92-93 -0.3494377838523768 1102 94-95 -0.3378443069608359 1102 96-97 -0.3551966174907015 1102 98-99 -0.40560989098799993 1102 100-101 -0.19420273606617044 1103 1 0.030913406744083716 1103 2 0.1740117559436669 1103 3 0.20044396723501734 1103 4 0.10497511580954466 1103 5 0.18873351636908353 1103 6 0.2124766568265244 1103 7 0.1845462219628331 1103 8 0.20064869736977187 1103 9 0.3040926607162504 1103 10-11 0.3018407105716463 1103 12-13 0.29611291605693424 1103 14-15 0.2779857362187599 1103 16-17 0.32424744495993707 1103 18-19 0.3443798013255517 1103 20-21 0.31648243761460293 1103 22-23 0.3058815128297283 1103 24-25 0.384390476889358 1103 26-27 0.31872214891702555 1103 28-29 0.3000575589603969 1103 30-31 0.22102306883636658 1103 32-33 0.2664672312627303 1103 34-35 0.18120347717653829 1103 36-37 0.2995027911490098 1103 38-39 0.4267779604695576 1103 40-41 0.26202045785205996 1103 42-43 0.16830703965371896 1103 44-45 0.24958036960691032 1103 46-47 0.27088556899582983 1103 48-49 0.25033453210343026 1103 50-51 0.0747856567153562 1103 52-53 0.11945368046085036 1103 54-55 0.36305249400140127 1103 56-57 0.3632229965237386 1103 58-59 0.2293293336648219 1103 60-61 0.28865448700560137 1103 62-63 0.28312235426761845 1103 64-65 0.08851780390661901 1103 66-67 0.10268063232148705 1103 68-69 0.15235384384014594 1103 70-71 0.18247681993549492 1103 72-73 0.0856416099441688 1103 74-75 0.04496283185137884 1103 76-77 -0.11192177377592216 1103 78-79 0.11958881086173179 1103 80-81 0.11251077298231138 1103 82-83 0.07565054461425547 1103 84-85 0.024654402972725364 1103 86-87 0.05948050197377697 1103 88-89 0.09904679268097993 1103 90-91 0.06528094771630677 1103 92-93 0.08630751985103657 1103 94-95 -0.014583981551876235 1103 96-97 0.03254881332283688 1103 98-99 -0.028029485031357382 1103 100-101 0.10146879447001211 1104 1 0.13917390142112396 1104 2 0.12275941780541189 1104 3 0.1850995220952143 1104 4 0.17785549352752383 1104 5 0.2264941883621816 1104 6 0.12734443467383727 1104 7 0.22153678596697546 1104 8 0.18758571887716613 1104 9 0.3085930118715865 1104 10-11 0.20368810054876718 1104 12-13 0.2501070472430058 1104 14-15 0.2946285869047287 1104 16-17 0.24365781690644894 1104 18-19 0.30274877171029146 1104 20-21 0.26676127581196596 1104 22-23 0.19155176753903191 1104 24-25 0.2951366993619047 1104 26-27 0.29889056440680406 1104 28-29 0.28452154293937326 1104 30-31 0.3613643848692192 1104 32-33 0.42682318456255075 1104 34-35 0.444091051840509 1104 36-37 0.35600855218258687 1104 38-39 0.4530803643190566 1104 40-41 0.6097595042393351 1104 42-43 0.44703954273067836 1104 44-45 0.5993663734591976 1104 46-47 0.7440114077703299 1104 48-49 0.7334079757203966 1104 50-51 0.6382672401825502 1104 52-53 0.687887944877339 1104 54-55 0.7172249842837957 1104 56-57 0.7612433148582198 1104 58-59 0.6740953807683141 1104 60-61 0.6274295111692609 1104 62-63 0.5924535250403622 1104 64-65 0.518427025037127 1104 66-67 0.6491250976266443 1104 68-69 0.6283042231574427 1104 70-71 0.4802591523022741 1104 72-73 0.5317686447328924 1104 74-75 0.6119036430549727 1104 76-77 0.5537187196494777 1104 78-79 0.5713899595916594 1104 80-81 0.473959653805057 1104 82-83 0.3737871614238166 1104 84-85 0.3950613954837259 1104 86-87 0.5237963401224555 1104 88-89 0.6712038251750201 1104 90-91 0.6748902613293133 1104 92-93 0.6739331187273372 1104 94-95 0.6233960756064789 1104 96-97 0.5768175062600953 1104 98-99 0.42569328304618637 1104 100-101 0.7642435474444014 1105 1 0.010583983211155612 1105 2 0.0939292795009834 1105 3 0.13757118018985182 1105 4 0.06031197269376776 1105 5 0.16272608715125614 1105 6 0.16059475824251024 1105 7 0.13321304110849752 1105 8 0.1292437457520066 1105 9 0.1235059702040715 1105 10-11 0.10257729437149266 1105 12-13 0.24509732324199973 1105 14-15 0.2859564382054387 1105 16-17 0.26611212765434544 1105 18-19 0.24476221590401792 1105 20-21 0.24991605344744272 1105 22-23 0.24018108997606902 1105 24-25 0.20123673121054964 1105 26-27 0.2005892152440154 1105 28-29 0.1987512800864124 1105 30-31 0.25351521869382765 1105 32-33 0.1905936574810383 1105 34-35 0.15175183667242464 1105 36-37 0.07887486241548913 1105 38-39 0.1141846991228519 1105 40-41 0.16449699009379515 1105 42-43 0.14846002615244203 1105 44-45 0.15704392965172786 1105 46-47 0.3629230650518096 1105 48-49 0.29212078226360205 1105 50-51 0.31645413751471807 1105 52-53 0.2981491376429588 1105 54-55 0.3447181571541691 1105 56-57 0.3167499115933978 1105 58-59 0.26630991188173425 1105 60-61 0.38889519256083815 1105 62-63 0.34826217567428586 1105 64-65 0.3107982582573001 1105 66-67 0.286372934290533 1105 68-69 0.3108438814960266 1105 70-71 0.12770821141200628 1105 72-73 0.3303154656283098 1105 74-75 0.37401900291077084 1105 76-77 0.17705750791020236 1105 78-79 0.1779301126006807 1105 80-81 0.24827914314253618 1105 82-83 0.2929324319001232 1105 84-85 0.3132329105982805 1105 86-87 0.38583631355933434 1105 88-89 0.3875150745227991 1105 90-91 0.35821681835532715 1105 92-93 0.4465324424518684 1105 94-95 0.44770743337517516 1105 96-97 0.46966138691577086 1105 98-99 0.5329310568937089 1105 100-101 0.8532518687497905 1106 1 0.18588470167534155 1106 2 0.1850291620227864 1106 3 0.23456150702776313 1106 4 0.13577517983852516 1106 5 0.1611723486422889 1106 6 0.2111692556866842 1106 7 0.1880276263089229 1106 8 0.22824559861398797 1106 9 0.2669374291750515 1106 10-11 0.19771384064610942 1106 12-13 0.3088290781297687 1106 14-15 0.2685910345824922 1106 16-17 0.32962551071577906 1106 18-19 0.35685919671100663 1106 20-21 0.3638773785026288 1106 22-23 0.3455321350042553 1106 24-25 0.3208916736622669 1106 26-27 0.29928714295800773 1106 28-29 0.32009223675375864 1106 30-31 0.328835461185939 1106 32-33 0.30294245482429716 1106 34-35 0.27350790857830276 1106 36-37 0.20829298792843787 1106 38-39 0.24130712296160084 1106 40-41 0.28151501967344217 1106 42-43 0.3067106649004714 1106 44-45 0.37337404204932767 1106 46-47 0.25558416834477526 1106 48-49 0.13276346182203014 1106 50-51 0.19339351315333886 1106 52-53 0.09025498359453366 1106 54-55 0.04462674996444349 1106 56-57 0.08679039096589491 1106 58-59 0.18841329937573192 1106 60-61 0.17481255631936676 1106 62-63 0.17337131148303087 1106 64-65 0.04494772045300266 1106 66-67 0.21507257056574502 1106 68-69 0.17592888199874324 1106 70-71 0.19021441183575405 1106 72-73 -0.013601079423757767 1106 74-75 0.10696065596295057 1106 76-77 0.07750087369896264 1106 78-79 0.11209021363794491 1106 80-81 0.006674349885127384 1106 82-83 0.027326403735418836 1106 84-85 0.1270930998527504 1106 86-87 0.14732651296496257 1106 88-89 0.31835191544489305 1106 90-91 0.2624003549066778 1106 92-93 0.29709208393125763 1106 94-95 0.2627789808372718 1106 96-97 0.3607588143202882 1106 98-99 0.39685801865767445 1106 100-101 0.6865922027860343 1107 1 -0.020600352262576394 1107 2 -0.03679138788420744 1107 3 0.01050875113579508 1107 4 0.024858736715550833 1107 5 -0.031046862226446592 1107 6 0.04896653942626017 1107 7 -0.06042649195125449 1107 8 -0.04235375863758151 1107 9 0.010678100867728801 1107 10-11 0.030079862340436136 1107 12-13 0.07185555971778257 1107 14-15 -0.022124905268206874 1107 16-17 0.03865130499347913 1107 18-19 0.10535481374405009 1107 20-21 0.06801449984730823 1107 22-23 -0.005368480679770471 1107 24-25 0.04750077913922013 1107 26-27 0.02318510716835931 1107 28-29 0.038509131999354906 1107 30-31 0.08822091848856672 1107 32-33 0.07669613841734702 1107 34-35 -0.04922976840515503 1107 36-37 -0.10378932314619504 1107 38-39 -0.07387576326336642 1107 40-41 -0.023693852587960862 1107 42-43 0.006838056482301624 1107 44-45 0.11120720187667388 1107 46-47 0.12474508329830769 1107 48-49 0.11564641388276442 1107 50-51 0.013658593383595985 1107 52-53 -0.0386238404082917 1107 54-55 -0.07594644501726577 1107 56-57 -0.035293879656450144 1107 58-59 -0.015866696914969225 1107 60-61 -0.08485949008205296 1107 62-63 -0.2072847215951441 1107 64-65 -4.8689002709778606E-4 1107 66-67 0.025191263769805516 1107 68-69 0.02409573979474544 1107 70-71 0.010725967083040189 1107 72-73 -0.0359009842157505 1107 74-75 -0.026876075528242893 1107 76-77 -0.017305821324498538 1107 78-79 0.010069445788960962 1107 80-81 0.08252281936086803 1107 82-83 0.04573805393894759 1107 84-85 -0.07171061095157327 1107 86-87 0.09832263037501932 1107 88-89 0.22825073648531458 1107 90-91 0.1861316106998281 1107 92-93 0.11186245808738349 1107 94-95 0.1156890350793951 1107 96-97 0.11326634305367733 1107 98-99 0.13658094616322103 1107 100-101 0.36129914130532725 1108 1 0.1938345091347955 1108 2 0.14365757353011332 1108 3 0.10603258713289421 1108 4 0.14007280957602575 1108 5 0.12236176627342132 1108 6 0.13723020316762558 1108 7 0.1575685148442716 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-1.575401225575014 2316 56-57 -1.4456088030295504 2316 58-59 -1.3079069264886556 2316 60-61 -1.172442428813035 2316 62-63 -1.2933452357159538 2316 64-65 -1.7596750747118293 2316 66-67 -1.7641463460777373 2316 68-69 -1.760541858133461 2316 70-71 -1.550243075143925 2316 72-73 -1.5273968973273888 2316 74-75 -1.6932176693244116 2316 76-77 -1.6148300302235512 2316 78-79 -1.4109167169425199 2316 80-81 -1.382941438877019 2316 82-83 -1.4759679646046528 2316 84-85 -1.5914683594246348 2316 86-87 -1.5075326544068766 2316 88-89 -1.7885439727731196 2316 90-91 -2.0111798560650804 2316 92-93 -2.1301158270448646 2316 94-95 -2.2420124830109067 2316 96-97 -2.5701953940635036 2316 98-99 -3.0466646174950753 2316 100-101 -3.461472935657479 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 10835.0 3 2631.0 4 2490.0 5 2767.0 6 3232.0 7 3663.0 8 4689.0 9 5543.0 10 6938.0 11 8784.0 12 11970.0 13 18316.0 14 17687.0 15 15467.0 16 19692.0 17 26269.0 18 22912.0 19 16916.0 20 17280.0 21 18668.0 22 20227.0 23 22560.0 24 26102.0 25 30076.0 26 35270.0 27 41812.0 28 50046.0 29 60682.0 30 74104.0 31 91419.0 32 116746.0 33 151717.0 34 207219.0 35 301263.0 36 468767.0 37 743853.0 38 709622.0 39 122111.0 40 44.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.756091388103506 19.345131145733415 14.788694575638104 23.11008289052498 2 14.868736839044184 20.660897371201532 40.8140876182526 23.65627817150169 3 19.221848569387998 24.66736898827199 30.193746676683542 25.917035765656472 4 12.739698605949865 16.853127430919123 37.88632366326537 32.520850299865636 5 13.673981159041732 37.29997593266622 34.74638619287858 14.279656715413475 6 31.32296690480202 36.29872145860969 17.788366006028504 14.58994563055978 7 27.65469227483792 32.92165124462624 21.615234204597357 17.808422275938483 8 25.087193546722425 35.295902095752815 20.376789025117272 19.24011533240749 9 25.71474353856787 17.693352554349932 21.296580362173223 35.29532354490898 10-11 24.50017719208563 26.544372290978202 27.84299213141694 21.112458385519233 12-13 25.725370559786736 24.1197338455891 28.07306059752877 22.081834997095395 14-15 22.69800323005226 25.496184272656556 26.7765144861913 25.029298011099883 16-17 21.770558649454117 28.650203952112097 27.378165448827225 22.201071949606565 18-19 21.692682975511072 27.893584291272404 29.083429448018943 21.330303285197576 20-21 22.625984223341977 26.79964579579252 28.795829731625155 21.778540249240343 22-23 22.20779711502609 26.647003843447425 28.86175923093979 22.283439810586696 24-25 22.393768159860247 26.77040232446211 28.906197971443344 21.929631544234297 26-27 22.176731379050892 26.684432006361526 29.267275285946294 21.871561328641288 28-29 21.85492394664087 27.201403509172206 29.068556683095885 21.875115861091036 30-31 22.061713669732555 26.93474229648541 28.85810247683117 22.145441556950864 32-33 22.012074563272687 27.011499251381636 28.860638643250592 22.115787542095077 34-35 22.470832932449518 26.936290280960822 28.627379051863187 21.96549773472647 36-37 22.396158766037967 26.698151721292806 28.96110779262264 21.944581720046582 38-39 22.181837399321797 26.98200914859936 28.98339236618 21.85276108589884 40-41 22.20368022130521 27.015582391506744 28.907133734197522 21.873603652990525 42-43 21.906072581124437 27.106127858661356 28.51188144830011 22.4759181119141 44-45 22.171995866220822 26.9928045600483 28.445585872960056 22.389613700770823 46-47 22.439094394057573 27.19006249894374 28.10822609155455 22.262617015444132 48-49 22.428925073706086 27.16055413395118 28.15276602126958 22.25775477107315 50-51 22.124572734751126 27.39485188973602 28.40635129264255 22.074224082870302 52-53 22.341535218486314 27.56261998505102 27.886653035534053 22.20919176092861 54-55 22.11396897953057 27.239497910059285 28.06229703412183 22.584236076288313 56-57 22.391093639273148 27.46075387442414 27.824917024441113 22.3232354618616 58-59 22.5825060971515 27.1500038874576 27.9548579545902 22.312632060800695 60-61 22.36517964550884 27.128116151423658 28.42389190386659 22.08281229920091 62-63 22.223818270819624 27.16050160672605 28.407314720956123 22.2083654014982 64-65 22.129689322113055 27.34157376128546 28.22230531415915 22.30643160244233 66-67 22.16941682505181 27.30837426989402 27.857383876165144 22.66482502888902 68-69 21.908382027733097 27.352182371667293 28.152627462982373 22.586808137617236 70-71 22.319147774708938 27.078077143117905 27.998949490283575 22.603825591889585 72-73 22.164121798379956 26.900714320507273 28.164643283868617 22.77052059724415 74-75 22.010358415740026 27.59042769151336 27.75421913756848 22.644994755178132 76-77 22.25379699403207 27.05795128480148 27.874130249443084 22.814121471723368 78-79 22.176367124902583 27.042091313222965 28.041007803959005 22.740533757915447 80-81 22.24041354201751 27.248200820714448 27.82199352043423 22.68939211683381 82-83 22.22784438843284 27.0671354913572 28.039840264280325 22.665179855929644 84-85 22.158468012112852 27.051263063844 27.931123681798947 22.859145242244196 86-87 22.09745911394816 27.08890165148284 28.088799964481158 22.724839270087838 88-89 22.20043091779302 26.954191565046326 27.823504184990412 23.02187333217024 90-91 22.17774070268194 26.80390468949822 28.018257335833468 23.00009727198637 92-93 22.25845175826114 27.008963908973204 27.937533014540794 22.795051318224857 94-95 22.158396550118553 26.859676645248175 27.935286069384453 23.04664073524882 96-97 21.923359150189807 26.774427759457687 28.07863219847471 23.22358089187779 98-99 22.05090566081527 26.862400215704135 27.728999040248393 23.3576950832322 100-101 22.283691376875325 26.261827755225987 28.153237288475474 23.301243579423215 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 3987.0 1 2327.0 2 760.0 3 815.0 4 715.0 5 751.5 6 1251.0 7 2510.0 8 4074.5 9 4763.0 10 4354.0 11 3935.0 12 4062.0 13 4428.5 14 4728.5 15 4972.0 16 5265.0 17 5479.5 18 5754.0 19 6064.5 20 6413.5 21 7167.0 22 8403.5 23 10193.0 24 12350.0 25 15141.5 26 19441.5 27 24524.5 28 29148.5 29 33914.5 30 39819.0 31 46809.0 32 53992.5 33 61281.5 34 69342.5 35 76734.5 36 85417.5 37 93848.5 38 98254.0 39 100860.5 40 102827.5 41 105415.0 42 108269.5 43 112756.0 44 119396.5 45 123960.0 46 127305.0 47 137528.0 48 163489.5 49 163165.0 50 137694.5 51 127746.0 52 123245.5 53 119565.5 54 115053.5 55 107694.5 56 97533.5 57 86843.5 58 78229.0 59 68557.0 60 56207.0 61 44246.0 62 34172.5 63 26189.0 64 19210.5 65 13478.0 66 9312.5 67 6602.5 68 4502.0 69 2888.5 70 1898.0 71 1153.5 72 678.5 73 445.5 74 292.5 75 184.5 76 126.5 77 83.5 78 63.0 79 53.5 80 52.0 81 37.0 82 28.5 83 28.0 84 22.5 85 17.5 86 15.5 87 9.5 88 6.5 89 8.0 90 9.5 91 10.0 92 6.0 93 4.0 94 3.0 95 3.0 96 3.0 97 1.0 98 1.0 99 1.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.27424311094867265 2 0.08133001784132755 3 0.08471995553769113 4 0.09927674682207585 5 0.10144174904832486 6 0.07748429020259578 7 0.0874831820632984 8 0.08437811308091497 9 0.08879357814760701 10-11 0.08249797956864609 12-13 0.08621551628608681 14-15 0.09471884739839373 16-17 0.08397929688134279 18-19 0.09383575438505534 20-21 0.09607197378979937 22-23 0.10743823547760661 24-25 0.11968759017875227 26-27 0.1228069025968347 28-29 0.11494452609098309 30-31 0.10800797290556688 32-33 0.10242454611155631 34-35 0.3677227794412528 36-37 0.5652934760221731 38-39 0.5484719784616463 40-41 0.513262205413702 42-43 0.5388719028005158 44-45 0.5593824502070853 46-47 0.5495117492676738 48-49 0.5698941057529522 50-51 0.5620317292471005 52-53 0.5654643972505612 54-55 0.5555082356969555 56-57 0.528360247254649 58-59 0.5238593215737629 60-61 0.47105890543754547 62-63 0.5448968761011956 64-65 0.5776852650803087 66-67 0.5908604431018898 68-69 0.5916153451939372 70-71 0.5883820852902627 72-73 0.5799642147921498 74-75 0.5777137519517068 76-77 0.5621314332969936 78-79 0.5399971342207374 80-81 0.5354677216684532 82-83 0.5483010572332583 84-85 0.5569753095739532 86-87 0.5493408280392856 88-89 0.5611913665408591 90-91 0.5747368738906143 92-93 0.58254227665367 94-95 0.5815309927190405 96-97 0.5780555944084829 98-99 0.5818016179973217 100-101 0.5600234048135406 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 3510389.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 49.986409736288564 #Duplication Level Percentage of deduplicated Percentage of total 1 72.13142155300605 36.055907926095166 2 14.488306654026884 14.484368655863674 3 5.315748712856349 7.971455796479579 4 2.6204999669108746 5.239575402397505 5 1.455583826472505 3.637970477778469 6 0.892033740902354 2.67537384428036 7 0.6505406051563525 2.2762732467606988 8 0.4283674208937163 1.7130039534776387 9 0.31631072136819793 1.4230113590062574 >10 1.5544954769099582 13.748303314797203 >50 0.09876327929172418 3.377620106259096 >100 0.04444155505129988 3.9097965025800208 >500 0.0014860437077762506 0.5128314282602228 >1k 0.0017146658108402903 1.73925564205748 >5k 1.71466581084029E-4 0.5913132057043491 >10k+ 1.1431105405601936E-4 0.6439391382021706 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 11954 0.3405320606918492 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 10585 0.3015335337479692 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 9021 0.2569800668814767 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 5862 0.16699004013515312 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5814 0.1656226703080485 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 4532 0.12910250117579564 No Hit GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 4479 0.12759269699170092 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 3870 0.11024419231031092 No Hit GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA 3627 0.10332188256059371 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 2.8486871398013156E-5 0.0 0.0 2.8486871398013156E-5 0.0 12-13 2.8486871398013156E-5 0.0 0.0 2.8486871398013156E-5 0.0 14-15 2.8486871398013156E-5 0.0 0.0 4.2730307097019735E-5 0.0 16-17 2.8486871398013156E-5 0.0 0.0 5.697374279602631E-5 0.0 18-19 4.2730307097019735E-5 0.0 0.0 1.281909212910592E-4 0.0 20-21 5.697374279602631E-5 0.0 0.0 1.7092122838807894E-4 0.0 22-23 5.697374279602631E-5 0.0 0.0 2.7062527828112496E-4 0.0 24-25 5.697374279602631E-5 0.0 0.0 4.415465066692039E-4 0.0 26-27 5.697374279602631E-5 0.0 0.0 9.827970632314538E-4 0.0 28-29 5.697374279602631E-5 0.0 0.0 0.0029768780610923747 0.0 30-31 5.697374279602631E-5 0.0 0.0 0.007976323991443683 0.0 32-33 7.121717849503289E-5 0.0 0.0 0.01838827548741749 0.0 34-35 8.546061419403946E-5 0.0 0.0 0.035466154890526375 0.0 36-37 1.1394748559205262E-4 0.0 0.0 0.06261414333283291 0.0 38-39 1.1394748559205262E-4 0.0 0.0 0.09946191148616293 0.0 40-41 1.1394748559205262E-4 0.0 0.0 0.1416224811552224 0.0 42-43 1.281909212910592E-4 0.0 0.0 0.18658900765698616 0.0 44-45 1.9940809978609208E-4 0.0 0.0 0.24470222530893299 0.0 46-47 2.563818425821184E-4 0.0 0.0 0.31636095031063505 0.0 48-49 2.8486871398013155E-4 0.0 0.0 0.3912529352160117 0.0 50-51 2.8486871398013155E-4 0.0 0.0 0.47080252359496344 0.0 52-53 2.9911214967913815E-4 0.0 0.0 0.5548103073477042 0.0 54-55 3.2759902107715123E-4 0.0 0.0 0.651879321636434 0.0 56-57 3.4184245677615783E-4 0.0 0.0 0.7714102340224972 0.0 58-59 3.4184245677615783E-4 0.0 0.0 0.9040166203802484 0.0 60-61 3.4184245677615783E-4 0.0 0.0 1.0539857548550886 0.0 62-63 3.4184245677615783E-4 0.0 0.0 1.2070884451837105 0.0 64-65 3.4184245677615783E-4 0.0 0.0 1.3678113736113007 0.0 66-67 3.70329328174171E-4 0.0 0.0 1.5581606482928245 0.0 68-69 3.70329328174171E-4 0.0 0.0 1.779816994640765 0.0 70-71 3.70329328174171E-4 0.0 0.0 2.020445597339782 0.0 72-73 3.70329328174171E-4 0.0 0.0 2.268694438137768 0.0 74-75 3.70329328174171E-4 0.0 0.0 2.536442542407693 0.0 76-77 3.70329328174171E-4 0.0 0.0 2.8270940912816216 0.0 78-79 3.70329328174171E-4 0.0 0.0 3.170560299727466 0.0 80-81 4.273030709701973E-4 0.0 0.0 3.540875384465938 0.0 82-83 4.273030709701973E-4 0.0 0.0 3.914566163465075 0.0 84-85 4.415465066692039E-4 0.0 0.0 4.314906410657052 0.0 86-87 4.557899423682105E-4 0.0 0.0 4.746069452701681 0.0 88-89 4.557899423682105E-4 0.0 0.0 5.208767461383909 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTACATG 10945 0.0 23.394533 1 TACATGG 11155 0.0 22.022457 2 GTATACG 130 2.9780736E-4 21.884888 1 GAGTACT 9705 0.0 21.313606 12-13 ACATGGG 11175 0.0 20.627632 3 AGTACTT 10555 0.0 19.933548 12-13 GTACTTT 10550 0.0 19.517183 14-15 GTGTAGC 1710 0.0 18.024103 1 TTTGCGT 710 0.0 18.000036 3 CTTTGCG 775 0.0 17.10111 2 CATGGGG 6360 0.0 17.04301 4 GTATCAA 31000 0.0 16.810183 1 TACTTTT 12320 0.0 16.809225 14-15 ACTTTTT 12640 0.0 16.59047 16-17 TATACCG 210 4.1144912E-4 15.777809 5 CATGGGA 5780 0.0 15.723215 4 AGAGTAC 21055 0.0 15.590247 10-11 CAACGCA 33830 0.0 15.278777 5 TCAACGC 34020 0.0 15.165618 4 TATCAAC 34190 0.0 15.14559 2 >>END_MODULE