FastQCFastQC Report
Thu 26 May 2016
SRR938870_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938870_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3510389
Sequences flagged as poor quality0
Sequence length101
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT115620.3293652071038281No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT114030.324835794551544No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT95810.27293271486436405No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT62240.1773022875812339No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT53240.15166410332302205No Hit
GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA43530.12400335119555127No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT40040.11406143307764467No Hit
GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA37350.10639846467157914No Hit
CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC36360.10357826440317583No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACATG110200.021.959221
GAGTACT103400.021.0878612-13
TTTGCGT8850.020.9101873
TACATGG111250.020.8993432
ACATGGG110850.019.8190063
TACCGTA1456.1932195E-419.63457
GTACTTT113250.018.91851614-15
AGTACTT113000.018.64541612-13
ACCGTAT2051.6053957E-518.5170888
CTTTGCG10150.017.7645472
CTCGCGC2550.016.74826660-61
TCGCGCT2600.016.42618460-61
ACTTTTT132300.016.24729716-17
AGAGTAC208600.015.95808910-11
GCACCGT5950.015.9495946
GTACCGT1800.002638501315.8166816
TACTTTT132850.015.7880414-15
GTATCAA307100.015.775241
GTATAGG10700.015.5818551
CATGGGG61350.015.3912924
Produced by FastQC (version 0.11.5)