##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938870_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 3510389 Sequences flagged as poor quality 0 Sequence length 101 %GC 44 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.08400949296502 33.0 31.0 34.0 30.0 34.0 2 32.330200442173215 34.0 31.0 34.0 30.0 34.0 3 32.42593684061795 34.0 31.0 34.0 30.0 34.0 4 36.01576434976295 37.0 35.0 37.0 35.0 37.0 5 35.92373580249938 37.0 35.0 37.0 35.0 37.0 6 35.9638088542324 37.0 35.0 37.0 35.0 37.0 7 35.903582480460145 37.0 35.0 37.0 35.0 37.0 8 35.86565562961825 37.0 35.0 37.0 35.0 37.0 9 37.53845485500325 39.0 37.0 39.0 35.0 39.0 10-11 37.449815675698616 39.0 37.0 39.0 34.0 39.0 12-13 37.37794244455529 39.0 37.0 39.0 34.0 39.0 14-15 38.6054830390592 40.0 38.0 41.0 34.0 41.0 16-17 38.594641363108195 40.0 38.0 41.0 34.0 41.0 18-19 38.70115448743715 40.0 38.0 41.0 34.0 41.0 20-21 38.69735177497422 40.0 38.0 41.0 34.0 41.0 22-23 38.635888928548944 40.0 38.0 41.0 34.0 41.0 24-25 38.568722298298 40.0 38.0 41.0 34.0 41.0 26-27 38.40770809161036 40.0 38.0 41.0 34.0 41.0 28-29 38.221947624607985 40.0 38.0 41.0 33.0 41.0 30-31 38.06821152869382 40.0 37.5 41.0 33.0 41.0 32-33 37.921181242306766 40.0 37.0 41.0 33.0 41.0 34-35 37.900815550641255 40.0 37.0 41.0 33.0 41.0 36-37 37.7985284536842 40.0 37.0 41.0 33.0 41.0 38-39 37.673048343075365 40.0 37.0 41.0 33.0 41.0 40-41 37.52935614827872 40.0 37.0 41.0 32.0 41.0 42-43 37.37375359255057 40.0 37.0 41.0 32.0 41.0 44-45 37.13365242427548 40.0 36.0 41.0 31.0 41.0 46-47 36.848153153396964 40.0 36.0 41.0 31.0 41.0 48-49 36.572459206087984 39.0 36.0 41.0 30.0 41.0 50-51 36.309475673493736 39.0 35.5 41.0 30.0 41.0 52-53 36.07404635212792 39.0 35.0 40.0 29.0 41.0 54-55 35.824310354208606 39.0 35.0 40.0 28.5 41.0 56-57 35.5400482681549 38.0 35.0 40.0 28.0 41.0 58-59 35.22318096370516 38.0 34.0 40.0 27.5 41.0 60-61 34.791656423262495 38.0 34.0 40.0 26.5 41.0 62-63 34.45557757274194 37.0 34.0 40.0 26.0 41.0 64-65 34.17687028417649 37.0 33.5 39.5 26.0 41.0 66-67 33.69297462474957 36.0 33.0 39.0 25.5 40.5 68-69 33.256609452684586 36.0 32.0 39.0 24.5 40.0 70-71 32.76768913644613 35.0 32.0 38.0 23.5 40.0 72-73 32.222539296926925 35.0 31.0 37.5 22.0 39.5 74-75 31.73208467779497 35.0 31.0 37.0 20.5 39.0 76-77 29.76658754912917 32.5 28.5 35.0 18.5 37.5 78-79 30.756727815635248 34.0 30.0 36.0 19.0 38.0 80-81 30.746927619702547 34.0 30.5 35.5 18.5 37.0 82-83 30.395569265970238 34.0 30.0 35.0 18.0 37.0 84-85 29.958487364220886 34.0 30.0 35.0 13.0 36.5 86-87 29.337859564851644 34.0 29.0 35.0 7.0 36.0 88-89 28.71981082438442 34.0 29.0 35.0 2.0 35.5 90-91 28.205105901368768 33.0 27.5 35.0 2.0 35.0 92-93 27.800038115433928 33.0 27.0 35.0 2.0 35.0 94-95 27.33065708672173 33.0 26.5 35.0 2.0 35.0 96-97 26.60620717533014 32.5 25.0 34.0 2.0 35.0 98-99 25.56186992381756 32.0 22.5 34.0 2.0 35.0 100-101 21.045879245861357 27.5 2.0 32.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.12557111501034512 1101 2 0.13174149560050807 1101 3 0.05888639530481754 1101 4 0.05354239194446819 1101 5 0.1325945460664144 1101 6 0.09084558971648704 1101 7 0.10308173515679186 1101 8 0.13895369470155572 1101 9 0.2454277075722615 1101 10-11 0.24043372468153024 1101 12-13 0.24709742917286803 1101 14-15 0.32297500590230044 1101 16-17 0.2707548184492268 1101 18-19 0.26996021178096186 1101 20-21 0.2324923918879236 1101 22-23 0.16453162556221912 1101 24-25 0.23090076967727668 1101 26-27 0.1964137797193004 1101 28-29 0.18613262927018326 1101 30-31 0.18310597098844994 1101 32-33 0.31819605307916987 1101 34-35 0.3937113405127022 1101 36-37 0.3076320205333687 1101 38-39 0.34113763854744406 1101 40-41 0.35772042550993177 1101 42-43 0.31339424745167577 1101 44-45 0.4120293446614127 1101 46-47 0.48889921697661975 1101 48-49 0.26443056443364554 1101 50-51 0.2510538063048031 1101 52-53 0.2819782971921896 1101 54-55 0.29886622567391186 1101 56-57 0.13219090981667847 1101 58-59 0.2396174297806155 1101 60-61 0.3895140751605126 1101 62-63 0.2770283820307782 1101 64-65 0.2193055423218766 1101 66-67 0.21521408189927627 1101 68-69 0.28048108009085126 1101 70-71 0.20137697102144614 1101 72-73 0.07695312831583578 1101 74-75 0.17087630910950224 1101 76-77 0.10387912335046323 1101 78-79 0.41245274458110615 1101 80-81 0.6555005500595819 1101 82-83 0.4768980876357567 1101 84-85 0.3868295976593039 1101 86-87 0.5384790673879358 1101 88-89 0.760100081863353 1101 90-91 0.6407041517741732 1101 92-93 0.8168051220832844 1101 94-95 0.7897363732768774 1101 96-97 0.6852365726398766 1101 98-99 0.6828699482179665 1101 100-101 1.0334925524085747 1102 1 0.14301519542172514 1102 2 0.10172958051376213 1102 3 0.08835919838166717 1102 4 0.13071224972568984 1102 5 0.056396213967801145 1102 6 0.10526066679747004 1102 7 0.07609741831945627 1102 8 0.11641997187977182 1102 9 0.2063404622300169 1102 10-11 0.1748356747934352 1102 12-13 0.16930608650910273 1102 14-15 0.250056747542331 1102 16-17 0.21437598789110268 1102 18-19 0.144267023859463 1102 20-21 0.16401726305078768 1102 22-23 0.19219349879511327 1102 24-25 0.12486782614853809 1102 26-27 0.16483726304854684 1102 28-29 0.2388005531330606 1102 30-31 0.18418744425801492 1102 32-33 0.18010952397695945 1102 34-35 0.29551747551780494 1102 36-37 0.17521802290584532 1102 38-39 0.20145542044445364 1102 40-41 0.15559373006018262 1102 42-43 0.13007280461616944 1102 44-45 0.12602633989572354 1102 46-47 0.2036380949702732 1102 48-49 0.14722151894378754 1102 50-51 0.10553158863738332 1102 52-53 0.13913460296731728 1102 54-55 0.07953023006339066 1102 56-57 0.09606083303953739 1102 58-59 0.05824189238104793 1102 60-61 0.05695871760428872 1102 62-63 0.09631384741643245 1102 64-65 0.05121561643638728 1102 66-67 0.009210153292848133 1102 68-69 0.09944851417762379 1102 70-71 0.06722743492650096 1102 72-73 0.14294003436614844 1102 74-75 0.20487347295354041 1102 76-77 -0.02403330216707289 1102 78-79 0.038607633332066627 1102 80-81 0.19203318253851975 1102 82-83 0.20641073354024542 1102 84-85 0.1995903938624899 1102 86-87 0.30129147397200384 1102 88-89 0.4636668975037317 1102 90-91 0.5363471624973997 1102 92-93 0.5322966404875622 1102 94-95 0.6665941976986574 1102 96-97 0.46721902598855536 1102 98-99 0.5641373997628705 1102 100-101 1.0190548183967287 1103 1 -0.004111957307955549 1103 2 0.057353974672054164 1103 3 0.10138120815118867 1103 4 0.13904650146781705 1103 5 0.07002463762267297 1103 6 0.056217901106499824 1103 7 0.06806439292595456 1103 8 0.13840500598296046 1103 9 0.12336950425276427 1103 10-11 0.15872723929371801 1103 12-13 0.13120033722308477 1103 14-15 0.20766345226692096 1103 16-17 0.15170787946933473 1103 18-19 0.25656705868270535 1103 20-21 0.22211332415096763 1103 22-23 0.20987567031490784 1103 24-25 0.18378935483616488 1103 26-27 0.1940398899676481 1103 28-29 0.12011737177436999 1103 30-31 0.23071494353003175 1103 32-33 0.15443539138032492 1103 34-35 0.18653568395517084 1103 36-37 0.09525369472510903 1103 38-39 0.2516105757811218 1103 40-41 0.22806256607298536 1103 42-43 0.20228319511185333 1103 44-45 0.3512479639687669 1103 46-47 0.35733646264387886 1103 48-49 0.1951392191871335 1103 50-51 0.19373681656063013 1103 52-53 0.3564470988080757 1103 54-55 0.4230089877080374 1103 56-57 0.2593068523976001 1103 58-59 0.2391740004338132 1103 60-61 0.3327849158108762 1103 62-63 0.49434014583227537 1103 64-65 0.4588785172161991 1103 66-67 0.5563200352423934 1103 68-69 0.524106276328169 1103 70-71 0.34571013791450156 1103 72-73 0.40399223331849754 1103 74-75 0.3608306861362358 1103 76-77 0.2827246695919463 1103 78-79 0.4827737627442552 1103 80-81 0.46819581765464235 1103 82-83 0.6438413907418443 1103 84-85 0.46364019990511807 1103 86-87 0.34887502599226394 1103 88-89 0.5967789706064899 1103 90-91 0.5160197535756801 1103 92-93 0.5347766523323934 1103 94-95 0.5903790020177269 1103 96-97 0.35758865589387057 1103 98-99 0.5288377561289579 1103 100-101 1.1393813646597089 1104 1 -0.0377262149099451 1104 2 -0.03947554462758518 1104 3 0.022419932588121583 1104 4 0.046871354921307784 1104 5 0.07818256712737082 1104 6 4.728607410200425E-4 1104 7 0.05682151164427296 1104 8 0.0895603418056723 1104 9 -0.012577328743319072 1104 10-11 0.036534222069725786 1104 12-13 0.09176611452801353 1104 14-15 0.029977461626430113 1104 16-17 0.07801897528258905 1104 18-19 0.07444190831173358 1104 20-21 0.04974125247355943 1104 22-23 0.025577181325886045 1104 24-25 0.08715714311383493 1104 26-27 0.06495605771559809 1104 28-29 0.05951039456122942 1104 30-31 0.0635330172517996 1104 32-33 0.10528529159658007 1104 34-35 0.12679129722536686 1104 36-37 0.17770340302281085 1104 38-39 0.28953361365722685 1104 40-41 0.21942049418696996 1104 42-43 0.13244848149467003 1104 44-45 0.17761746977847537 1104 46-47 0.06154910979882544 1104 48-49 0.10509621135045677 1104 50-51 0.10993863700551287 1104 52-53 0.04204312414688616 1104 54-55 0.1262938546514576 1104 56-57 0.06429377968410677 1104 58-59 0.11284847596071046 1104 60-61 0.19931564016436454 1104 62-63 0.1961159140280344 1104 64-65 0.13946901446605864 1104 66-67 0.09317239702279778 1104 68-69 0.2340392786715242 1104 70-71 0.006429415299400887 1104 72-73 -0.13473029138406645 1104 74-75 -0.05223455226694895 1104 76-77 -0.11580465014020191 1104 78-79 0.19904355434573162 1104 80-81 0.25756450294297295 1104 82-83 0.41503152918744135 1104 84-85 0.26567603165651477 1104 86-87 0.21244631052543284 1104 88-89 0.4140396460763789 1104 90-91 0.5806544129550026 1104 92-93 0.4214725587651422 1104 94-95 0.4948033854098206 1104 96-97 0.5661563245701196 1104 98-99 0.720000288897662 1104 100-101 1.0553464160118295 1105 1 -0.06199013852741331 1105 2 0.004104243002942098 1105 3 -0.006735129652263083 1105 4 -0.02245941696785536 1105 5 0.02478797597587601 1105 6 0.06792422379523799 1105 7 -0.01656386372927443 1105 8 0.03421959519213402 1105 9 -0.08248713609290093 1105 10-11 -0.023440502303976984 1105 12-13 -0.06018004093402851 1105 14-15 0.01593000714626669 1105 16-17 0.018651408044675577 1105 18-19 0.05690855193895317 1105 20-21 0.06524205546597273 1105 22-23 0.05332836209743874 1105 24-25 0.05559022369090627 1105 26-27 0.04747335424003296 1105 28-29 0.04788038771715719 1105 30-31 0.0785098422807664 1105 32-33 0.11263656859169657 1105 34-35 0.10399207243492725 1105 36-37 0.15465023611557882 1105 38-39 0.20083937419607878 1105 40-41 0.26145460611801496 1105 42-43 0.27987043537512335 1105 44-45 0.10907037783076134 1105 46-47 0.29836360160965825 1105 48-49 0.19344587558443038 1105 50-51 0.23907337032393627 1105 52-53 0.4401096742610662 1105 54-55 0.35574266981412705 1105 56-57 0.35534261653095456 1105 58-59 0.3740082070768409 1105 60-61 0.30648073811588716 1105 62-63 0.2731559213464436 1105 64-65 0.3190347209299773 1105 66-67 0.3682255910352481 1105 68-69 0.2655472442207767 1105 70-71 0.3172901418550751 1105 72-73 0.3027365894139109 1105 74-75 0.30657583310164327 1105 76-77 0.2812069983320775 1105 78-79 0.45718071358217927 1105 80-81 0.42648293653177305 1105 82-83 0.6097275440411103 1105 84-85 0.5199672976740111 1105 86-87 0.3940062842333596 1105 88-89 0.5776325797431063 1105 90-91 0.7805891909970555 1105 92-93 0.7452343580102614 1105 94-95 0.7406002113874699 1105 96-97 0.4455424588900847 1105 98-99 0.6075927007887252 1105 100-101 1.3847093414792262 1106 1 0.04048261551626808 1106 2 -0.06185740973524645 1106 3 -0.06965920105842827 1106 4 -0.010657484473227896 1106 5 -0.01852747452812764 1106 6 0.021888102506430585 1106 7 0.038958029069284805 1106 8 0.09488629481638355 1106 9 0.020131087825149052 1106 10-11 0.047473932015293485 1106 12-13 0.07295316137064134 1106 14-15 0.06049239000627438 1106 16-17 -0.031692984784768896 1106 18-19 -0.04910533300189712 1106 20-21 0.054208766747855464 1106 22-23 0.12179147872018348 1106 24-25 0.08171626239758467 1106 26-27 0.1347828780575142 1106 28-29 0.12414814681636699 1106 30-31 0.04858634674155837 1106 32-33 -3.606636393485019E-4 1106 34-35 -0.004401439107631688 1106 36-37 -0.02152455420571897 1106 38-39 0.07938701866971343 1106 40-41 0.09730304287636216 1106 42-43 0.17159997197257582 1106 44-45 0.07794354079342725 1106 46-47 0.014716428017500505 1106 48-49 -0.04652647344840233 1106 50-51 0.020493568824349495 1106 52-53 0.15152767317680116 1106 54-55 0.12142761246291656 1106 56-57 0.12235561896569891 1106 58-59 0.20470803742322374 1106 60-61 0.2862666886320824 1106 62-63 0.25030717193642005 1106 64-65 0.2169965811005241 1106 66-67 0.30134836326683256 1106 68-69 0.23515639957024348 1106 70-71 0.28109853982591204 1106 72-73 0.2602200541155071 1106 74-75 0.37928893310641953 1106 76-77 0.24598662900895363 1106 78-79 0.40772116506321865 1106 80-81 0.5438521146887787 1106 82-83 0.5659570617658858 1106 84-85 0.427316265372351 1106 86-87 0.3067233575197825 1106 88-89 0.48348885283498433 1106 90-91 0.8159353642040763 1106 92-93 0.9522240435114071 1106 94-95 0.9949794224564066 1106 96-97 0.9872377407559192 1106 98-99 1.0540661335804735 1106 100-101 1.4646915093110415 1107 1 -0.10288620367370527 1107 2 -0.059703333896976574 1107 3 -0.12484761765575314 1107 4 -0.1057027659704417 1107 5 -0.11429170443216918 1107 6 -0.03347335851891842 1107 7 -0.09010256711157183 1107 8 -0.05557742984324676 1107 9 -0.03794059133939953 1107 10-11 -0.07919077090762272 1107 12-13 -0.05329185426587202 1107 14-15 -0.05207200337522977 1107 16-17 -0.10750734050688493 1107 18-19 -0.12205442714116543 1107 20-21 -0.16903029429730765 1107 22-23 -0.0734543312255056 1107 24-25 -0.14677694611214065 1107 26-27 -0.16486339630518643 1107 28-29 -0.05941884265116926 1107 30-31 -0.1711831399534418 1107 32-33 -0.008910040255607043 1107 34-35 -0.1059010595763894 1107 36-37 -0.08082273400827233 1107 38-39 -0.03473028480799911 1107 40-41 -0.009741384308398437 1107 42-43 0.023710388080644407 1107 44-45 -0.15134330552222508 1107 46-47 -0.2703490489897007 1107 48-49 -0.31646268954077783 1107 50-51 -0.17744676609466126 1107 52-53 -0.07733109378513348 1107 54-55 -0.2125720510151794 1107 56-57 -0.15463354225976644 1107 58-59 -0.09577278607356021 1107 60-61 -0.15476452020745057 1107 62-63 -0.21934413347062787 1107 64-65 -0.22056635415928838 1107 66-67 -0.19916972100882901 1107 68-69 -0.13811335546111536 1107 70-71 -0.08982815001125743 1107 72-73 -0.14894030696158467 1107 74-75 -0.21751201083330685 1107 76-77 -0.44013813669501545 1107 78-79 -0.20247716885966227 1107 80-81 0.05962597029403227 1107 82-83 0.07241744302589481 1107 84-85 -0.026057030985537466 1107 86-87 -0.16777407303900915 1107 88-89 -0.05713173215085021 1107 90-91 0.1470765636738669 1107 92-93 0.30621042234694684 1107 94-95 0.1138341318538032 1107 96-97 0.13953345371028902 1107 98-99 0.1890144363478008 1107 100-101 0.821839431665957 1108 1 0.039599886105769144 1108 2 -0.014750659176449687 1108 3 -0.018931306563828798 1108 4 -0.07199022407752409 1108 5 0.026293261385262667 1108 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-1.3622890162395933 2316 60-61 -1.2326424083896086 2316 62-63 -1.270496336200516 2316 64-65 -1.262848763021502 2316 66-67 -1.391427388213664 2316 68-69 -1.4436285779012579 2316 70-71 -1.4820038266143172 2316 72-73 -1.3843987138878013 2316 74-75 -1.6566451859645106 2316 76-77 -1.6149720786370025 2316 78-79 -1.6221135567943996 2316 80-81 -1.7590691510845708 2316 82-83 -2.0105090074311924 2316 84-85 -2.1381762893859566 2316 86-87 -2.138914825980013 2316 88-89 -2.331145587478975 2316 90-91 -2.6694257605971607 2316 92-93 -2.9677667719852217 2316 94-95 -3.121556009769609 2316 96-97 -3.3425985547705004 2316 98-99 -3.6339071741354374 2316 100-101 -4.309688483063816 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 3.0 3 10.0 4 18.0 5 67.0 6 164.0 7 414.0 8 984.0 9 1864.0 10 3177.0 11 4777.0 12 6841.0 13 9459.0 14 11428.0 15 15275.0 16 19613.0 17 27455.0 18 27391.0 19 20657.0 20 19495.0 21 20998.0 22 23288.0 23 25879.0 24 30226.0 25 35929.0 26 42585.0 27 52003.0 28 62806.0 29 75973.0 30 92181.0 31 112687.0 32 139436.0 33 177214.0 34 235493.0 35 336010.0 36 521354.0 37 783276.0 38 540127.0 39 33831.0 40 1.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 41.38114751402656 20.2870715425341 15.43637980198235 22.89540114145699 2 15.371373372010908 21.45841956546696 39.69773720234424 23.47246986017789 3 19.26362007173564 24.9534168435464 30.46363237806408 25.319330706653876 4 13.266250549440532 17.715016768796847 37.203996480162175 31.814736201600454 5 13.580583482320394 37.60661263250732 34.62379485706928 14.189009028103005 6 31.199248858174975 36.91029683604866 17.368160622654642 14.52229368312173 7 27.541278188827505 33.103168908061186 21.349314848012572 18.006238055098738 8 25.063718009599505 35.778940738476564 20.027552502016157 19.129788749907775 9 25.491419896769273 17.63998234953448 21.09882978780984 35.7697679658864 10-11 24.518607481962825 26.807969145299854 27.605359406037337 21.068063966699988 12-13 25.62667556216704 24.250360857443436 28.020598286970475 22.102365293419048 14-15 22.565642483166165 25.735405762574064 26.609466145881555 25.089485608378215 16-17 21.66653799795858 28.883755751277597 27.27665984769993 22.173046403063893 18-19 21.58891792334126 28.041607354626507 29.063801761001418 21.305672961030815 20-21 22.549025193504196 26.891649330031512 28.81701999408043 21.742305482383863 22-23 22.07337990177157 26.765851305937886 28.8704756082588 22.290293184031743 24-25 22.274126884513368 26.96711105236485 28.923646353723193 21.83511570939859 26-27 22.123474065124977 26.82463396506769 29.25929291597028 21.79259905383705 28-29 21.815502498441056 27.303441299525495 29.0511108597936 21.82994534223985 30-31 22.059734690371922 27.01830196026708 28.849608974959757 22.072354374401243 32-33 21.974359536792075 27.105429056437906 28.8747486389685 22.045462767801517 34-35 22.41582849115874 26.99359570954416 28.679960106007947 21.910615693289152 36-37 22.328644425502432 26.817697246368223 28.99239002110464 21.8612683070247 38-39 22.159478362865332 27.076457937642694 29.018864801331183 21.745198898160787 40-41 22.16902173200855 27.060917196392566 28.90149747628839 21.868563595310494 42-43 21.896827311788197 27.209462714297395 28.49905722108258 22.394652752831824 44-45 22.153052637696156 27.078824079713442 28.44741044686407 22.320712835726336 46-47 22.419131951279333 27.314572016297955 28.09092693410028 22.17536909832243 48-49 22.41154017842518 27.26194237146473 28.112619748465907 22.213897701644182 50-51 22.11861652229921 27.486467954526745 28.343186517892267 22.05172900528178 52-53 22.34564317515808 27.656094523997197 27.78985462864657 22.208407672198152 54-55 22.102607431825934 27.349347892783392 27.972441230872136 22.575603444518542 56-57 22.380468375442153 27.554496097156182 27.72188495349091 22.343150573910755 58-59 22.54220159768914 27.290715470465116 27.885137873152 22.281945058693747 60-61 22.383187906810985 27.296377111229457 28.25817346718869 22.06226151477086 62-63 22.189763014868152 27.27447015131371 28.306976805134703 22.22879002868343 64-65 22.112821199429405 27.4992789237055 28.11684783189272 22.27105204497237 66-67 22.160623233254682 27.429886401284893 27.757874666805684 22.651615698654744 68-69 21.86104566147998 27.45699624508558 28.06426057015104 22.617697523283404 70-71 22.242292805726088 27.235542841548334 27.83355348937112 22.688610863354462 72-73 22.145010139901874 27.01412863360727 28.00642606844996 22.83443515804089 74-75 21.993303306271756 27.680792641499274 27.57462207179888 22.75128198043009 76-77 22.17067496062296 27.15101317891972 27.77866431081801 22.899647549639315 78-79 22.050826041755244 27.173411581149704 27.92718919332364 22.84857318377141 80-81 22.12905749191899 27.328538233227146 27.728793019804925 22.81361125504894 82-83 22.102735622747222 27.206657153950744 27.90659382763563 22.784013395666406 84-85 21.976595756196822 27.17948067863704 27.80901204966173 23.034911515504408 86-87 21.870171150853157 27.22256685912288 27.94089349569131 22.966368494332652 88-89 21.95663073900265 27.07622630065225 27.690708804244657 23.276434156100443 90-91 21.940266960415254 26.887199142191626 27.88014405990139 23.29238983749173 92-93 21.95742409174596 27.13243461052322 27.790766208531302 23.119375089199515 94-95 21.800917220285275 27.030038551283063 27.765512597036967 23.403531631394696 96-97 21.490817631992535 26.928949634639547 27.985462496962572 23.594770236405346 98-99 21.58330600967585 27.006907211707876 27.583737870646246 23.82604890797003 100-101 21.763149239961958 26.27390995884068 28.279103978240556 23.68383682295681 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 1398.0 1 954.5 2 539.5 3 617.5 4 606.5 5 617.0 6 1011.5 7 2080.0 8 3432.0 9 4082.5 10 3697.0 11 3120.5 12 3090.0 13 3310.5 14 3567.5 15 3817.5 16 4003.0 17 4230.0 18 4529.5 19 4954.0 20 5632.5 21 6772.5 22 8177.5 23 10036.5 24 12445.5 25 15449.0 26 19897.5 27 25009.0 28 29421.5 29 34251.5 30 40256.0 31 47335.0 32 54740.5 33 61944.0 34 69972.0 35 77248.0 36 86018.5 37 94315.0 38 98444.0 39 101321.5 40 103685.0 41 106626.0 42 109405.5 43 113673.0 44 120491.0 45 125556.0 46 128296.5 47 138775.5 48 165251.0 49 165274.5 50 140415.0 51 128973.0 52 123279.0 53 119943.0 54 115679.5 55 107952.5 56 97385.5 57 86642.0 58 77598.5 59 67678.0 60 55607.5 61 43623.5 62 33461.5 63 25515.5 64 18970.0 65 13245.5 66 8859.5 67 6236.5 68 4122.0 69 2599.0 70 1698.5 71 955.5 72 537.0 73 292.0 74 176.5 75 116.5 76 56.5 77 25.0 78 19.0 79 13.0 80 10.0 81 7.0 82 6.5 83 4.0 84 1.0 85 1.5 86 1.0 87 0.0 88 0.0 89 0.0 90 0.5 91 0.5 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.3882760571549193 2 0.0 3 0.0 4 0.0 5 4.8427681376622363E-4 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0013673698271046313 16-17 0.0023359234546370786 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0024498709402291314 36-37 0.007435073434881433 38-39 0.01251997997942678 40-41 0.002364410326035092 42-43 6.267111707562894E-4 44-45 4.2730307097019735E-4 46-47 2.8486871398013155E-4 48-49 2.8486871398013155E-4 50-51 5.127636851642367E-4 52-53 0.0 54-55 0.0 56-57 0.0 58-59 1.851646640870855E-4 60-61 0.002350166890336085 62-63 0.0 64-65 3.275990210771513E-4 66-67 0.0014243435699006577 68-69 0.0015952647982887369 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.12025732760671252 78-79 9.970404989304605E-5 80-81 0.0 82-83 0.0 84-85 0.0 86-87 0.14498393198018794 88-89 0.4321885694149566 90-91 0.33345307314944295 92-93 0.0 94-95 0.0 96-97 5.697374279602631E-4 98-99 0.0 100-101 1.5667779268907235E-4 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 3510389.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 48.84342784024545 #Duplication Level Percentage of deduplicated Percentage of total 1 70.9854074833881 34.67170628125286 2 15.036325346438883 14.688513440824824 3 5.541200255882251 8.119536445396031 4 2.7530055362090953 5.378649090065006 5 1.5239169050784136 3.7216662693863833 6 0.956870460456515 2.8042099972662133 7 0.6352092291522122 2.1718057303287805 8 0.42173425695933764 1.6479157398042354 9 0.33602367993802373 1.4771293527299936 >10 1.653695852899287 14.16118368472184 >50 0.10644372701360842 3.569025856144719 >100 0.046314119474071075 3.9684709848710766 >500 0.00186819254407542 0.6269941936644702 >1k 0.0016930494829244713 1.7358397476996432 >5k 1.751430499577039E-4 0.6024995569396747 >10k+ 1.1676203330513594E-4 0.6548536289043321 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 11562 0.3293652071038281 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 11403 0.324835794551544 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 9581 0.27293271486436405 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6224 0.1773022875812339 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 5324 0.15166410332302205 No Hit GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 4353 0.12400335119555127 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 4004 0.11406143307764467 No Hit GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA 3735 0.10639846467157914 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 3636 0.10357826440317583 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 2.8486871398013156E-5 0.0 10-11 2.8486871398013156E-5 0.0 0.0 5.697374279602631E-5 0.0 12-13 8.546061419403946E-5 0.0 0.0 5.697374279602631E-5 0.0 14-15 1.1394748559205262E-4 0.0 0.0 7.121717849503289E-5 0.0 16-17 1.281909212910592E-4 0.0 0.0 8.546061419403946E-5 0.0 18-19 1.4243435699006578E-4 0.0 0.0 1.5667779268907235E-4 0.0 20-21 1.4243435699006578E-4 0.0 0.0 1.8516466408708548E-4 0.0 22-23 1.4243435699006578E-4 0.0 0.0 2.7062527828112496E-4 0.0 24-25 1.4243435699006578E-4 0.0 0.0 4.2730307097019735E-4 0.0 26-27 1.4243435699006578E-4 0.0 0.0 9.543101918334407E-4 0.0 28-29 2.1365153548509868E-4 0.0 0.0 0.0029626346253933677 0.0 30-31 2.563818425821184E-4 0.0 0.0 0.008047541169938716 0.0 32-33 2.563818425821184E-4 0.0 0.0 0.01860192702290259 0.0 34-35 2.8486871398013155E-4 0.0 0.0 0.03593618826859359 0.0 36-37 3.4184245677615783E-4 0.0 0.0 0.06339753229627827 0.0 38-39 3.70329328174171E-4 0.0 0.0 0.10033076106380234 0.0 40-41 3.70329328174171E-4 0.0 0.0 0.14264800852555087 0.0 42-43 3.9881619957218416E-4 0.0 0.0 0.1877569693843047 0.0 44-45 3.9881619957218416E-4 0.0 0.0 0.24628324667152274 0.0 46-47 3.9881619957218416E-4 0.0 0.0 0.3184262484869911 0.0 48-49 4.273030709701973E-4 0.0 0.0 0.39421556984140504 0.0 50-51 4.273030709701973E-4 0.0 0.0 0.474277921905521 0.0 52-53 4.273030709701973E-4 0.0 0.0 0.5585563309365429 0.0 54-55 4.273030709701973E-4 0.0 0.0 0.656109622039039 0.0 56-57 4.273030709701973E-4 0.0 0.0 0.7756690212965002 0.0 58-59 4.273030709701973E-4 0.0 0.0 0.9090018228749008 0.0 60-61 4.273030709701973E-4 0.0 0.0 1.0587573058142559 0.0 62-63 4.273030709701973E-4 0.0 0.0 1.2114896668147033 0.0 64-65 4.273030709701973E-4 0.0 0.0 1.3717425618642265 0.0 66-67 4.273030709701973E-4 0.0 0.0 1.5613939081964991 0.0 68-69 4.273030709701973E-4 0.0 0.0 1.7825090039878773 0.0 70-71 4.415465066692039E-4 0.0 0.0 2.0234224754008743 0.0 72-73 4.700333780672171E-4 0.0 0.0 2.272169836448325 0.0 74-75 4.8427681376622363E-4 0.0 0.0 2.5406585993745994 0.0 76-77 4.8427681376622363E-4 0.0 0.0 2.832050806904876 0.0 78-79 4.8427681376622363E-4 0.0 0.0 3.174762113258673 0.0 80-81 5.127636851642368E-4 0.0 0.0 3.54356739381305 0.0 82-83 5.127636851642368E-4 0.0 0.0 3.915164387764433 0.0 84-85 5.270071208632434E-4 0.0 0.0 4.313496310522851 0.0 86-87 5.554939922612565E-4 0.0 0.0 4.742679515005317 0.0 88-89 5.982242993582763E-4 0.0 0.0 5.202628540597638 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTACATG 11020 0.0 21.95922 1 GAGTACT 10340 0.0 21.08786 12-13 TTTGCGT 885 0.0 20.910187 3 TACATGG 11125 0.0 20.899343 2 ACATGGG 11085 0.0 19.819006 3 TACCGTA 145 6.1932195E-4 19.6345 7 GTACTTT 11325 0.0 18.918516 14-15 AGTACTT 11300 0.0 18.645416 12-13 ACCGTAT 205 1.6053957E-5 18.517088 8 CTTTGCG 1015 0.0 17.764547 2 CTCGCGC 255 0.0 16.748266 60-61 TCGCGCT 260 0.0 16.426184 60-61 ACTTTTT 13230 0.0 16.247297 16-17 AGAGTAC 20860 0.0 15.958089 10-11 GCACCGT 595 0.0 15.949594 6 GTACCGT 180 0.0026385013 15.816681 6 TACTTTT 13285 0.0 15.78804 14-15 GTATCAA 30710 0.0 15.77524 1 GTATAGG 1070 0.0 15.581855 1 CATGGGG 6135 0.0 15.391292 4 >>END_MODULE