FastQCFastQC Report
Thu 26 May 2016
SRR938869_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938869_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2909874
Sequences flagged as poor quality0
Sequence length101
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT151380.5202287109338755No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT150150.5160017237859783No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT115270.39613399068138344No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT93370.3208729999993127No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT79980.2748572618608228No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT76130.2616264484304131No Hit
CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC63900.21959713719563115No Hit
GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA55920.19217326935805468No Hit
GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT47000.16151902109850805No Hit
GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA46800.16083170611511016No Hit
TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT41450.14244603030921615No Hit
ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC35740.1228231875332059No Hit
ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC35680.12261699303818653No Hit
TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC35110.1206581453355025No Hit
GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG33820.11622496369258602No Hit
GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT33000.11340697226065459No Hit
GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA31140.10701494291505405No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTTGCGT8750.025.9884663
CTTTGCG9650.023.5646692
GTACATG113300.022.3704031
GAGTACT110100.022.04244612-13
TACATGG110850.021.9672092
TTGCGTT11550.021.7390884
ACATGGG113100.020.776223
AGTACTT116650.020.25671812-13
GTACTTT123400.019.55294614-15
CATGGGA70350.018.1822284
GTATAGC13150.018.047071
CTATACG1901.9049685E-417.4538764
GTGTAGC17000.017.0311281
CATGGGG43900.016.726874
TAGCCCT19950.016.622744
ACTTTTT148950.016.50297516-17
TACTTTT144300.016.49122414-15
TATCACG2304.372268E-516.4781932
AGTACAT87050.016.1090682
GTAGGAC6250.015.9179353
Produced by FastQC (version 0.11.5)