FastQCFastQC Report
Thu 26 May 2016
SRR938869_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938869_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2909874
Sequences flagged as poor quality0
Sequence length101
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT179120.6155592991311651No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT148130.5090598424536595No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT126220.43376448602241885No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT83960.28853483003044117No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT68900.23678001178057884No Hit
GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA54650.18780881921347797No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT54140.18605616600581332No Hit
GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT51170.1758495385023544No Hit
CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC50180.17244732933453477No Hit
GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA48170.1655398137513858No Hit
TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC44820.15402728777947086No Hit
ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC44550.15309941255188367No Hit
GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG41800.14364883153016247No Hit
GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA38460.13217067130741744No Hit
TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT37470.1287684621395978No Hit
GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA30040.10323471050636558No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT29440.10117276555617184No Hit
ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC29390.10100093681032238No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTAAGCG3950.024.6312994-95
GTACATG111950.022.4168471
TACATGG111400.022.1964192
GAGTACT124050.022.03756312-13
ACATGGG111450.021.2921893
TTTGCGT7700.020.3401263
GTACTTT134500.020.20184514-15
AGTACTT129550.019.92963412-13
CATGGGG43650.017.8315874
CTTTGCG9150.017.1168272
ACTTTTT161400.017.06997316-17
CATGGGA68300.016.7466034
TTGCGTT9950.016.2175874
TACTTTT157550.016.19190414-15
TACATCG3001.4807374E-615.8200975
AGAGTAC244750.015.590910-11
GTAGTAC8900.015.4645893
AGTACAT85500.014.987462
CCGATCG1950.00448850414.6031669
TACACTG22100.014.6031665
Produced by FastQC (version 0.11.5)