##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938869_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2909874 Sequences flagged as poor quality 0 Sequence length 101 %GC 43 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.887446672948727 31.0 31.0 34.0 30.0 34.0 2 32.15163611895223 33.0 31.0 34.0 30.0 34.0 3 32.22999346363451 34.0 31.0 34.0 30.0 34.0 4 35.85668073600438 37.0 35.0 37.0 35.0 37.0 5 35.774296756491864 37.0 35.0 37.0 35.0 37.0 6 35.82657359047162 37.0 35.0 37.0 35.0 37.0 7 35.75858611060135 37.0 35.0 37.0 33.0 37.0 8 35.725132772071916 37.0 35.0 37.0 33.0 37.0 9 37.34018826932025 39.0 37.0 39.0 34.0 39.0 10-11 37.228030492041924 39.0 37.0 39.0 33.0 39.0 12-13 37.11785372837449 39.0 37.0 39.0 33.0 39.0 14-15 38.30082556839231 40.0 38.0 41.0 33.0 41.0 16-17 38.41566610787959 40.0 38.0 41.0 33.5 41.0 18-19 38.473157772467125 40.0 38.0 41.0 33.5 41.0 20-21 38.445011708410746 40.0 38.0 41.0 34.0 41.0 22-23 38.34987374023755 40.0 38.0 41.0 34.0 41.0 24-25 38.24616667250884 40.0 38.0 41.0 33.0 41.0 26-27 38.032984074224515 40.0 37.0 41.0 33.0 41.0 28-29 37.838263615538 40.0 37.0 41.0 33.0 41.0 30-31 37.60885093306446 40.0 37.0 41.0 32.0 41.0 32-33 37.46177532085582 39.5 36.0 41.0 32.0 41.0 34-35 37.31375293225754 39.0 36.0 41.0 31.5 41.0 36-37 37.1379094077613 39.0 36.0 41.0 31.0 41.0 38-39 36.95329385396069 39.0 36.0 41.0 31.0 41.0 40-41 36.75483972845559 39.0 35.0 41.0 30.0 41.0 42-43 36.525625508183516 39.0 35.0 40.0 30.0 41.0 44-45 36.22323200248533 39.0 35.0 40.0 30.0 41.0 46-47 35.840979025208654 38.5 35.0 40.0 28.5 41.0 48-49 35.422130648955935 38.0 35.0 40.0 27.0 41.0 50-51 35.10977107599848 38.0 34.0 40.0 26.5 41.0 52-53 34.80028035578173 38.0 34.0 40.0 26.0 41.0 54-55 34.45653609056612 38.0 33.5 40.0 24.5 41.0 56-57 34.26443584842505 38.0 33.0 40.0 24.5 41.0 58-59 33.92705027777835 37.5 33.0 40.0 23.0 41.0 60-61 33.46751285450847 37.0 32.5 39.5 22.5 41.0 62-63 32.96892734874431 36.0 31.5 39.0 21.5 40.0 64-65 32.47793598623171 36.0 31.0 39.0 19.5 40.0 66-67 31.927387577606453 35.0 31.0 38.5 18.0 40.0 68-69 31.341124907813878 35.0 30.0 38.0 14.5 40.0 70-71 30.699118930922783 34.0 29.0 37.0 10.0 39.0 72-73 30.072388185880214 34.0 29.0 36.5 7.5 39.0 74-75 29.456649325709634 34.0 28.0 36.0 7.0 39.0 76-77 27.680713151153626 31.5 26.0 34.0 2.0 36.0 78-79 28.635038664904393 33.0 27.5 35.0 2.0 37.0 80-81 28.552106895350107 33.0 27.5 35.0 2.0 37.0 82-83 28.00509918986183 33.0 26.5 35.0 2.0 36.0 84-85 27.39250101550789 32.5 26.0 35.0 2.0 36.0 86-87 26.50627363933971 32.0 24.5 34.5 2.0 35.0 88-89 25.58791755244385 31.0 22.0 34.0 2.0 35.0 90-91 24.762916882311742 31.0 19.0 34.0 2.0 35.0 92-93 24.104243173415757 31.0 16.5 34.0 2.0 35.0 94-95 23.222043634879036 30.0 4.5 34.0 2.0 35.0 96-97 21.95680637718334 29.5 2.0 34.0 2.0 35.0 98-99 20.286219265851372 29.0 2.0 34.0 2.0 35.0 100-101 15.00209115583699 12.5 2.0 28.5 2.0 32.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.0930399385030114 1101 2 0.12122114760358471 1101 3 0.1916040051236152 1101 4 0.08585646010422465 1101 5 0.04129364175391004 1101 6 0.10426582894127279 1101 7 0.18145009464443262 1101 8 0.028783505461859704 1101 9 0.24523510789163083 1101 10-11 0.28574582639102175 1101 12-13 0.22764761438886438 1101 14-15 0.2490521574034048 1101 16-17 0.2903940135548311 1101 18-19 0.2521083892950742 1101 20-21 0.2361065038289425 1101 22-23 0.2580316665570166 1101 24-25 0.19512669580051778 1101 26-27 0.28215138180940613 1101 28-29 0.19076857205530473 1101 30-31 0.20308967192529082 1101 32-33 0.23172193722970036 1101 34-35 0.3637620596701723 1101 36-37 0.44230525949495814 1101 38-39 0.3083701685052489 1101 40-41 0.34638738240211353 1101 42-43 0.29769036270802474 1101 44-45 0.4042966283546079 1101 46-47 0.46681930693748797 1101 48-49 0.558094743389006 1101 50-51 0.5304661140650992 1101 52-53 0.44757189788020213 1101 54-55 0.47786382809704264 1101 56-57 0.43852299057606103 1101 58-59 0.4817034025392033 1101 60-61 0.5591540246786764 1101 62-63 0.5129219776117395 1101 64-65 0.6204698875302199 1101 66-67 0.5736089609725603 1101 68-69 0.6376322394410856 1101 70-71 0.8407658929717066 1101 72-73 0.8053742409372475 1101 74-75 0.721795348348639 1101 76-77 0.5130924443707663 1101 78-79 0.9478744653062954 1101 80-81 1.0196380942811452 1101 82-83 0.904305816280921 1101 84-85 1.0731597890130722 1101 86-87 1.284913001061767 1101 88-89 1.3191243500286411 1101 90-91 1.2629046521263625 1101 92-93 1.1543177136400935 1101 94-95 1.1755002544302506 1101 96-97 1.3303076100476616 1101 98-99 1.4160025736901005 1101 100-101 1.404427492010111 1102 1 0.22621996510646625 1102 2 0.16593810579568213 1102 3 0.22600031502366136 1102 4 0.1634753812368288 1102 5 0.12524295115085238 1102 6 0.22498758639036254 1102 7 0.2095336985684071 1102 8 0.2108227921506085 1102 9 0.20037281935953644 1102 10-11 0.24926171579850376 1102 12-13 0.22861459032778697 1102 14-15 0.3754618036208299 1102 16-17 0.39983685884926246 1102 18-19 0.32293253622792406 1102 20-21 0.3796955256318526 1102 22-23 0.37267710570115753 1102 24-25 0.35889385439619303 1102 26-27 0.4041429082503569 1102 28-29 0.34198459721630314 1102 30-31 0.40441962523113517 1102 32-33 0.35678534178523336 1102 34-35 0.42730381426427755 1102 36-37 0.33498557946951735 1102 38-39 0.4227161703591733 1102 40-41 0.4321357678285054 1102 42-43 0.4266440642821081 1102 44-45 0.38823911006505796 1102 46-47 0.5082119304030996 1102 48-49 0.49044469626635134 1102 50-51 0.4506852427903496 1102 52-53 0.48406910885417176 1102 54-55 0.5631960344103675 1102 56-57 0.5059714337990115 1102 58-59 0.4766075477580287 1102 60-61 0.5520718164938714 1102 62-63 0.4981781113652204 1102 64-65 0.47813056467667536 1102 66-67 0.3553613815745713 1102 68-69 0.49425314641081997 1102 70-71 0.6450652316541259 1102 72-73 0.7553443359285659 1102 74-75 0.7166479955162579 1102 76-77 0.45514384278162723 1102 78-79 0.7804417812614233 1102 80-81 0.8409240457934715 1102 82-83 0.7680670119443427 1102 84-85 0.8960818684604988 1102 86-87 1.0456961638061948 1102 88-89 1.0299671808158521 1102 90-91 1.1547883011208064 1102 92-93 1.4187625297997961 1102 94-95 1.522607961249136 1102 96-97 1.453642215989479 1102 98-99 1.669767352275585 1102 100-101 1.3474964609365188 1103 1 0.05567446961269695 1103 2 0.053553032990869553 1103 3 0.04029383508129314 1103 4 0.041714680611214305 1103 5 0.0916456291704364 1103 6 0.04493888349211517 1103 7 0.11697822960147164 1103 8 0.12560167766552865 1103 9 0.08076651178397043 1103 10-11 0.03933059853661547 1103 12-13 0.0915802973963622 1103 14-15 0.1121362632630607 1103 16-17 0.13594028148830262 1103 18-19 0.08341575189884765 1103 20-21 0.1464049960806051 1103 22-23 0.1728732097252248 1103 24-25 0.14066161860557003 1103 26-27 0.14370870259002544 1103 28-29 0.0773930502274709 1103 30-31 0.18217886963241625 1103 32-33 0.20261245289463403 1103 34-35 0.2080868995404188 1103 36-37 0.12916279352553772 1103 38-39 0.26848646568608103 1103 40-41 0.3783289184555869 1103 42-43 0.33179804912867183 1103 44-45 0.38253763349452896 1103 46-47 0.4192026492123233 1103 48-49 0.24512841534557595 1103 50-51 0.34269654708365493 1103 52-53 0.4216075138918356 1103 54-55 0.581653677860622 1103 56-57 0.6546636470936491 1103 58-59 0.5203679847788933 1103 60-61 0.49369628720495484 1103 62-63 0.5564632344596845 1103 64-65 0.4712095322161787 1103 66-67 0.42862456533139337 1103 68-69 0.5006340450471143 1103 70-71 0.3901185476356126 1103 72-73 0.46400528774647753 1103 74-75 0.7294479259041289 1103 76-77 0.5922243305272055 1103 78-79 0.9239016320816056 1103 80-81 0.923414483722869 1103 82-83 0.8749317404762316 1103 84-85 1.045984168348017 1103 86-87 1.1957868259275983 1103 88-89 1.0394305915798867 1103 90-91 0.9024703067691142 1103 92-93 1.0510976200716264 1103 94-95 1.2598503227894575 1103 96-97 1.0552020907388275 1103 98-99 1.390920802234831 1103 100-101 1.3662359439384915 1104 1 0.06174200179239264 1104 2 0.08759856026057378 1104 3 0.10092268891562384 1104 4 0.11993033603301484 1104 5 -0.020229579793728192 1104 6 0.049270478369557225 1104 7 0.10776913573303659 1104 8 0.1286120208799133 1104 9 0.11388104271885169 1104 10-11 0.185093293325842 1104 12-13 0.22215130554337037 1104 14-15 0.24561617846217842 1104 16-17 0.1851582911609455 1104 18-19 0.21383948556815824 1104 20-21 0.29276144816980576 1104 22-23 0.22925100055769576 1104 24-25 0.15152126961731938 1104 26-27 0.1637069899496666 1104 28-29 0.16705909165447963 1104 30-31 0.03779560590658093 1104 32-33 0.17582877259421537 1104 34-35 0.2838515273465205 1104 36-37 0.33194627485744377 1104 38-39 0.3637144325993802 1104 40-41 0.47300342548906116 1104 42-43 0.32264625315450957 1104 44-45 0.45309123819320263 1104 46-47 0.4691854283616763 1104 48-49 0.3934072432699267 1104 50-51 0.3255226051585325 1104 52-53 0.33376486776174374 1104 54-55 0.6203418457195227 1104 56-57 0.6089976031480475 1104 58-59 0.5647233551342126 1104 60-61 0.49440294024604725 1104 62-63 0.5619279424198709 1104 64-65 0.6732521076998594 1104 66-67 0.606502792824184 1104 68-69 0.6044137541627066 1104 70-71 0.5121822037072086 1104 72-73 0.46067867274866003 1104 74-75 0.6165656592246158 1104 76-77 0.5422524442742294 1104 78-79 0.6952518664048064 1104 80-81 0.623674339118093 1104 82-83 0.8403308643851339 1104 84-85 0.9585745305903401 1104 86-87 0.9929380501821115 1104 88-89 1.030385835627989 1104 90-91 1.0405021303973818 1104 92-93 1.3100692425024327 1104 94-95 1.4462246316729583 1104 96-97 1.3718484512836255 1104 98-99 1.5765065409778387 1104 100-101 1.5656431311093044 1105 1 0.0594466824870139 1105 2 0.11846094397686358 1105 3 0.09564576010748738 1105 4 0.07020267808696445 1105 5 0.05218725560526849 1105 6 0.03811265530661956 1105 7 0.0962535696513811 1105 8 0.06916202289104234 1105 9 0.21802824288165112 1105 10-11 0.15428078061531636 1105 12-13 0.22792230815984027 1105 14-15 0.20490433375999118 1105 16-17 0.12444829845238559 1105 18-19 0.18506410319976396 1105 20-21 0.19538160565442553 1105 22-23 0.15744871879628164 1105 24-25 0.036183917950673106 1105 26-27 0.022874966147917064 1105 28-29 0.1882455604727582 1105 30-31 0.1780381995296878 1105 32-33 0.242130771105856 1105 34-35 0.3214093441943717 1105 36-37 0.3210764631179899 1105 38-39 0.3043320361371613 1105 40-41 0.4320212999842852 1105 42-43 0.3880820248751178 1105 44-45 0.35140713943808777 1105 46-47 0.39648615766875395 1105 48-49 0.4467804341459356 1105 50-51 0.4115061083811611 1105 52-53 0.33262948790444824 1105 54-55 0.3424576569710496 1105 56-57 0.378652950641829 1105 58-59 0.46833072045524915 1105 60-61 0.557112221478306 1105 62-63 0.4559582577261665 1105 64-65 0.29598039254199904 1105 66-67 0.4106834742250136 1105 68-69 0.48007581369239816 1105 70-71 0.592029631008625 1105 72-73 0.6124642740369879 1105 74-75 0.7354924076137408 1105 76-77 0.4580443739175202 1105 78-79 0.4985829864512077 1105 80-81 0.6306633596026749 1105 82-83 0.6742008331305449 1105 84-85 0.5964740640708897 1105 86-87 0.7270621229792447 1105 88-89 1.0002530346530207 1105 90-91 0.9577920697740403 1105 92-93 1.3006280220611792 1105 94-95 1.3234675269028742 1105 96-97 1.3004876782880466 1105 98-99 1.4400585920858475 1105 100-101 1.1498922385373405 1106 1 0.14371276685493228 1106 2 0.10284498760848493 1106 3 0.03938701216667795 1106 4 -0.007888045480655137 1106 5 0.08535672416191886 1106 6 0.07158575675246226 1106 7 0.15953255369225872 1106 8 0.11280906420488179 1106 9 0.1397309942407574 1106 10-11 0.19353266747240383 1106 12-13 0.1415955735574883 1106 14-15 0.05604576091165825 1106 16-17 -0.014277614675037853 1106 18-19 0.011817977907305988 1106 20-21 0.13959820109418075 1106 22-23 0.21190183331499668 1106 24-25 0.20202342745299973 1106 26-27 0.15754740507225762 1106 28-29 0.22544960388803048 1106 30-31 0.17278318706917162 1106 32-33 0.30744702987617956 1106 34-35 0.1488982417620477 1106 36-37 0.05198144206325139 1106 38-39 0.12711487259802823 1106 40-41 0.3394781731101304 1106 42-43 0.2554597918106367 1106 44-45 0.32154095301371655 1106 46-47 0.33548591235028624 1106 48-49 0.2886900288830745 1106 50-51 0.06986993244965589 1106 52-53 0.08830435800563663 1106 54-55 0.2740052882304198 1106 56-57 0.30439176891307085 1106 58-59 0.2515465359240423 1106 60-61 0.2703959241383984 1106 62-63 0.4291153447254459 1106 64-65 0.3996406236088603 1106 66-67 0.37906157600221846 1106 68-69 0.3337977473887719 1106 70-71 0.47196203352437394 1106 72-73 0.5224721234818261 1106 74-75 0.41909518716022376 1106 76-77 0.42472750290790984 1106 78-79 0.8549086778594805 1106 80-81 0.7759571679411899 1106 82-83 0.639608007694715 1106 84-85 0.7977714792000867 1106 86-87 1.1107115416316802 1106 88-89 1.2885870288417216 1106 90-91 1.381006644351661 1106 92-93 1.280116140776915 1106 94-95 1.443220487603302 1106 96-97 1.463671456881233 1106 98-99 1.6229555681534293 1106 100-101 1.986137314294492 1107 1 -0.01153633815538413 1107 2 -0.009066975139980116 1107 3 0.01144184771537482 1107 4 0.01368828664055144 1107 5 0.04275423848337567 1107 6 -0.06624102706859958 1107 7 0.04215570090897103 1107 8 0.12519513546331495 1107 9 -0.020762712345870682 1107 10-11 -0.007218483616178162 1107 12-13 0.08075689307450062 1107 14-15 0.055852058991241904 1107 16-17 0.08398731529385373 1107 18-19 -0.0352445351824926 1107 20-21 0.0193136491750181 1107 22-23 -0.017704392926674473 1107 24-25 0.11718849729963665 1107 26-27 0.18372809152582192 1107 28-29 0.018994826504169282 1107 30-31 0.11100583901666283 1107 32-33 0.14690991669673537 1107 34-35 0.13568798782262093 1107 36-37 0.2552002579530992 1107 38-39 0.19894898562228036 1107 40-41 0.020021384915857254 1107 42-43 0.15365125612119357 1107 44-45 0.19495593414811907 1107 46-47 0.32497554848367116 1107 48-49 0.19248745465508676 1107 50-51 0.015539527976166312 1107 52-53 -0.03238441780907664 1107 54-55 0.023249199641902862 1107 56-57 0.11226442265108005 1107 58-59 0.1303564139623674 1107 60-61 0.345248965692619 1107 62-63 0.5103676032247364 1107 64-65 0.37208828882248923 1107 66-67 0.30805978439510184 1107 68-69 0.24759279664983325 1107 70-71 0.4729999936749536 1107 72-73 0.5023265811761277 1107 74-75 0.5093147051517164 1107 76-77 0.6318371772607954 1107 78-79 0.7666636172584482 1107 80-81 0.6620485019234472 1107 82-83 0.603155151052583 1107 84-85 0.9054800560172787 1107 86-87 0.9052279867619468 1107 88-89 0.9028136481517954 1107 90-91 0.8408729086392093 1107 92-93 0.9561168526450388 1107 94-95 1.1397796469912258 1107 96-97 1.42696304392393 1107 98-99 1.3317282575933689 1107 100-101 1.012384738384501 1108 1 0.04167059257590111 1108 2 0.07039663191757484 1108 3 0.06350875651531851 1108 4 0.07583914582041018 1108 5 0.0018996493003342607 1108 6 0.12985039220286865 1108 7 0.12906962803933197 1108 8 0.15627517757809528 1108 9 0.13667647697618435 1108 10-11 0.09660180367614402 1108 12-13 0.2076604395963031 1108 14-15 0.28189587943391103 1108 16-17 0.2046220763978397 1108 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-1.2053134683618225 2316 92-93 -1.296905995015024 2316 94-95 -1.2821909005371914 2316 96-97 -1.0098576298923412 2316 98-99 -0.9928421561333138 2316 100-101 -0.20759603123613246 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 3.0 3 14.0 4 24.0 5 104.0 6 201.0 7 492.0 8 1229.0 9 2271.0 10 3786.0 11 5724.0 12 8149.0 13 11335.0 14 14315.0 15 19043.0 16 24262.0 17 33233.0 18 33124.0 19 25308.0 20 25176.0 21 27007.0 22 29956.0 23 33723.0 24 38284.0 25 44381.0 26 52304.0 27 62304.0 28 74334.0 29 88144.0 30 105830.0 31 127040.0 32 155018.0 33 192805.0 34 251190.0 35 338623.0 36 446306.0 37 462796.0 38 168264.0 39 3772.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 41.65987590054525 21.195742698302727 15.499982584831699 21.644398816320322 2 15.612669139625975 22.701189123652775 39.10348008195544 22.582661654765808 3 20.00193135510335 26.020920493464665 31.09196480672359 22.885183344708395 4 13.236037024283526 18.242783020845575 37.84981755223766 30.67136240263324 5 12.809076956596746 38.52768195461384 34.79250304308709 13.870738045702321 6 29.869059622512868 37.939202865828555 17.37126762189703 14.82046988976155 7 26.289866846468268 34.27955987097723 21.743243865541945 17.687329417012556 8 24.93015161481219 35.35331770379061 20.189843271564335 19.526687409832867 9 25.252227416032447 17.862354177534833 21.76688750097083 35.11853090546189 10-11 24.30385301906543 27.21621279821738 27.756923495656512 20.72301068706068 12-13 25.934542182926133 24.88800202345531 28.087728197165923 21.08972759645263 14-15 22.44875165386532 26.27638881729299 27.509407701427907 23.765451827413784 16-17 20.77755555925952 29.34598392294174 28.267094783367302 21.609365734431442 18-19 20.607596450549664 28.419676004919825 30.454912076495805 20.517815468034705 20-21 21.832508899010747 27.041566060935974 30.141442550433457 20.984482489619825 22-23 21.260611284199936 27.043026605275696 30.104379089953724 21.59198302057065 24-25 21.58834025115864 27.2260070367308 30.293236064516883 20.892416647593677 26-27 21.57092422933922 26.828129612529484 30.848545623812445 20.752400534318852 28-29 20.798666883858203 27.79039573534799 30.5444668738234 20.866470506970405 30-31 21.227620164996836 27.300632261053227 30.20974447690862 21.262003097041315 32-33 21.21026546166604 27.344019019380223 30.278871181363865 21.166844337589875 34-35 21.867525555104542 27.118794073339824 30.134091906607914 20.87958846494772 36-37 21.7472202337934 26.926612797193723 30.53617111964918 20.7899958493637 38-39 21.36960938588814 27.423601407704503 30.525686104717913 20.681103101689448 40-41 21.44207919609623 27.450307187867896 30.224663938895453 20.882949677140424 42-43 21.053780173720344 27.681791531459577 29.73137750735978 21.533050787460297 44-45 21.575866964989764 27.33077521448488 29.542509580917446 21.550848239607905 46-47 21.88694009769616 27.78225110813729 29.057940682608447 21.2728681115581 48-49 21.829051090500595 27.716388224225994 29.183574401910796 21.270986283362618 50-51 21.22704438092083 28.09115165228221 29.407506355988993 21.274297610807967 52-53 21.67214113050943 28.433430450940488 28.583127654324553 21.31130076422553 54-55 21.2132724647184 27.785172141474167 28.98039571472854 22.021159679078888 56-57 21.662845195358972 28.073071205144966 28.647838360011463 21.616245239484595 58-59 22.198135435252976 27.697151191402437 28.64324871952075 21.461464653823842 60-61 21.608206246958588 27.839115797750523 29.42602871918379 21.126649236107102 62-63 21.506903971239723 27.686094835914055 29.306850153977166 21.500151038869053 64-65 21.292668778111356 28.219540760779914 28.996537973013098 21.49125248809563 66-67 21.425302927906447 27.859366738612955 28.576372439501625 22.13895789397897 68-69 21.14104425408005 27.993045568947277 28.849187031615063 22.01672314535761 70-71 21.67889772527912 27.835058809257518 28.569592458228854 21.916451007234507 72-73 21.57949107074739 27.325186588835116 29.00476103089 22.090561309527494 74-75 21.145176732738253 28.50984269421975 28.345196389946782 21.999784183095215 76-77 21.437444927557166 27.641946749375933 28.54683728547972 22.37377103758718 78-79 21.37906124529219 27.824644450408982 28.71414132492677 22.082152979372058 80-81 21.511438395626197 27.894815250369387 28.543521094839104 22.050225259165313 82-83 21.364773869933888 27.80177079832323 28.754217536566873 22.07923779517601 84-85 21.231419696213127 27.88158161071856 28.515201199779096 22.37179749328922 86-87 21.11191347412081 27.784525920118234 28.65764744910761 22.445913156653344 88-89 21.278529516803278 27.64598814048539 28.317049648130926 22.758432694580407 90-91 21.17653936369553 27.295849125061522 28.663534360351804 22.86407715089115 92-93 21.291334263957822 27.814589222763598 28.514224327238914 22.37985218603967 94-95 21.15123235001703 27.608771182814767 28.397455261393468 22.842541205774737 96-97 20.645498739807977 27.438507646722847 28.75301473534593 23.162978878123244 98-99 20.933140060360003 27.535023853266498 28.104155025269133 23.427681061104362 100-101 21.120884000076977 26.48742346721359 29.153888291686485 23.237804241022946 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 2799.0 1 1945.0 2 1173.0 3 1298.0 4 1111.0 5 1070.5 6 1901.5 7 3789.0 8 5851.0 9 6434.5 10 5233.5 11 4058.0 12 3919.0 13 4160.5 14 4459.5 15 4663.0 16 4937.0 17 5300.0 18 5652.0 19 6069.0 20 6723.0 21 7757.5 22 9163.0 23 11061.0 24 13328.0 25 16547.5 26 21222.0 27 26273.0 28 30130.5 29 34321.0 30 39116.5 31 44233.0 32 49881.0 33 56342.0 34 64328.0 35 70728.5 36 77127.0 37 83171.5 38 85527.0 39 86895.0 40 89152.0 41 92281.0 42 94814.5 43 95931.0 44 98420.5 45 101193.5 46 103444.0 47 116978.0 48 148677.0 49 147803.5 50 115548.0 51 98857.0 52 90558.5 53 85722.5 54 81230.0 55 74452.0 56 66119.0 57 57266.0 58 49843.0 59 43686.0 60 37158.0 61 29956.0 62 23437.5 63 17919.0 64 13097.0 65 9352.5 66 6384.5 67 4382.5 68 2958.0 69 1812.5 70 1154.5 71 745.0 72 451.5 73 291.0 74 185.5 75 127.0 76 81.0 77 41.5 78 23.5 79 9.5 80 7.5 81 5.5 82 4.0 83 4.0 84 2.5 85 2.0 86 2.0 87 1.0 88 0.5 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.3471971638634525 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 8.247779800774879E-4 16-17 0.0025946140623270973 18-19 5.154862375484299E-4 20-21 0.0 22-23 0.0 24-25 0.0 26-27 3.436574916989533E-5 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.012955887437050539 36-37 0.023420258059283668 38-39 0.01080802811393208 40-41 0.005928091731806944 42-43 0.0048112048837853465 44-45 0.0018385675805894 46-47 0.001237166970116232 48-49 0.002972637303195946 50-51 6.873149833979066E-4 52-53 0.0 54-55 0.0 56-57 0.0 58-59 8.935094784172786E-4 60-61 0.0014433614651356039 62-63 9.278752275871738E-4 64-65 6.18583485058116E-4 66-67 0.0022165908214582487 68-69 0.002560248313157202 70-71 1.7182874584947665E-5 72-73 0.0 74-75 0.0 76-77 0.08041585305755508 78-79 0.002852357181101312 80-81 1.2028012209463365E-4 82-83 0.0 84-85 1.7182874584947664E-4 86-87 0.08555353255845442 88-89 0.40479415947219705 90-91 0.35785054610612005 92-93 0.0 94-95 9.278752275871738E-4 96-97 0.0 98-99 0.0 100-101 3.436574916989533E-4 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2909874.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 47.836400674736254 #Duplication Level Percentage of deduplicated Percentage of total 1 70.94450074297379 33.93729563210018 2 15.155815839938485 14.499993581436238 3 5.688811387655208 8.163967827086307 4 2.804663502785992 5.3666002830832005 5 1.5185740231002725 3.6321557711635415 6 0.9180102780698832 2.6348584491166194 7 0.6063925620808345 2.030534629611507 8 0.41895058884659525 1.6032870584785937 9 0.32398742338719294 1.3948552978852657 >10 1.4581332624403627 12.138498379660028 >50 0.10104955913545435 3.348636582639287 >100 0.05478014789193937 4.744858974288294 >500 0.0030934012823725155 0.958926560369536 >1k 0.0025898243293072058 2.735788397393894 >5k 4.3163738821786764E-4 1.2492027324636892 >10k+ 2.1581869410893382E-4 1.560539843223992 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 17912 0.6155592991311651 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 14813 0.5090598424536595 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 12622 0.43376448602241885 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 8396 0.28853483003044117 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 6890 0.23678001178057884 No Hit GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 5465 0.18780881921347797 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 5414 0.18605616600581332 No Hit GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT 5117 0.1758495385023544 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 5018 0.17244732933453477 No Hit GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA 4817 0.1655398137513858 No Hit TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC 4482 0.15402728777947086 No Hit ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC 4455 0.15309941255188367 No Hit GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG 4180 0.14364883153016247 No Hit GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA 3846 0.13217067130741744 No Hit TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT 3747 0.1287684621395978 No Hit GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA 3004 0.10323471050636558 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2944 0.10117276555617184 No Hit ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC 2939 0.10100093681032238 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 3.436574916989533E-5 2 0.0 0.0 0.0 3.436574916989533E-5 3.436574916989533E-5 3 0.0 0.0 0.0 3.436574916989533E-5 3.436574916989533E-5 4 0.0 0.0 0.0 1.0309724750968598E-4 3.436574916989533E-5 5 0.0 0.0 0.0 1.0309724750968598E-4 3.436574916989533E-5 6 0.0 0.0 0.0 1.0309724750968598E-4 3.436574916989533E-5 7 0.0 0.0 0.0 1.0309724750968598E-4 3.436574916989533E-5 8 0.0 0.0 0.0 1.0309724750968598E-4 3.436574916989533E-5 9 0.0 0.0 0.0 1.7182874584947664E-4 3.436574916989533E-5 10-11 0.0 0.0 0.0 1.7182874584947664E-4 3.436574916989533E-5 12-13 0.0 0.0 0.0 2.405602441892673E-4 3.436574916989533E-5 14-15 0.0 0.0 0.0 2.5774311877421497E-4 3.436574916989533E-5 16-17 0.0 0.0 0.0 4.8112048837853464E-4 3.436574916989533E-5 18-19 0.0 0.0 0.0 6.701321088129589E-4 6.873149833979066E-5 20-21 0.0 0.0 0.0 8.24777980077488E-4 6.873149833979066E-5 22-23 0.0 0.0 0.0 9.79423851342017E-4 1.03097247509686E-4 24-25 0.0 0.0 0.0 0.0012887155938710749 1.3746299667958132E-4 26-27 0.0 0.0 0.0 0.001890116204344243 1.7182874584947664E-4 28-29 0.0 0.0 0.0 0.003969244029122911 1.7182874584947664E-4 30-31 0.0 0.0 0.0 0.01001761588302449 1.7182874584947664E-4 32-33 0.0 0.0 0.0 0.023385892310113773 1.7182874584947664E-4 34-35 0.0 0.0 0.0 0.042750991967349786 2.0619449501937197E-4 36-37 0.0 0.0 0.0 0.07166976989381671 2.0619449501937197E-4 38-39 0.0 0.0 0.0 0.11636242668926558 2.0619449501937197E-4 40-41 0.0 0.0 0.0 0.16519615625968687 2.0619449501937197E-4 42-43 0.0 0.0 0.0 0.2174149121233428 2.2337736960431962E-4 44-45 0.0 0.0 0.0 0.28255518967487936 2.405602441892673E-4 46-47 0.0 0.0 0.0 0.365101719180968 2.5774311877421497E-4 48-49 0.0 0.0 0.0 0.4505865202410826 2.7492599335916264E-4 50-51 0.0 0.0 0.0 0.542721093765572 2.7492599335916264E-4 52-53 0.0 0.0 0.0 0.6392201174346381 2.9210886794411026E-4 54-55 0.0 0.0 0.0 0.7520428719594044 3.0929174252905794E-4 56-57 0.0 0.0 0.0 0.8927877976847107 3.0929174252905794E-4 58-59 0.0 0.0 0.0 1.0488082989160357 3.0929174252905794E-4 60-61 0.0 0.0 0.0 1.2216336514914392 3.436574916989533E-4 62-63 0.0 0.0 0.0 1.4024490407488435 3.436574916989533E-4 64-65 0.0 0.0 0.0 1.5929383883975734 3.436574916989533E-4 66-67 0.0 0.0 0.0 1.8188244576912953 3.436574916989533E-4 68-69 0.0 0.0 0.0 2.0773579886964177 3.436574916989533E-4 70-71 0.0 0.0 0.0 2.361167528215998 3.436574916989533E-4 72-73 0.0 0.0 0.0 2.6512316340844997 4.1238899003874394E-4 74-75 0.0 0.0 0.0 2.972035902585473 4.1238899003874394E-4 76-77 0.0 0.0 0.0 3.3054180352826275 4.1238899003874394E-4 78-79 0.0 0.0 0.0 3.701328648594407 4.1238899003874394E-4 80-81 0.0 0.0 0.0 4.1346807456267864 4.295718646236916E-4 82-83 0.0 0.0 0.0 4.563324735022891 4.467547392086393E-4 84-85 0.0 0.0 0.0 5.0187224601477585 4.639376137935869E-4 86-87 0.0 0.0 0.0 5.501389407238939 4.811204883785346E-4 88-89 0.0 0.0 0.0 6.018645480869619 4.811204883785346E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTAAGCG 395 0.0 24.63129 94-95 GTACATG 11195 0.0 22.416847 1 TACATGG 11140 0.0 22.196419 2 GAGTACT 12405 0.0 22.037563 12-13 ACATGGG 11145 0.0 21.292189 3 TTTGCGT 770 0.0 20.340126 3 GTACTTT 13450 0.0 20.201845 14-15 AGTACTT 12955 0.0 19.929634 12-13 CATGGGG 4365 0.0 17.831587 4 CTTTGCG 915 0.0 17.116827 2 ACTTTTT 16140 0.0 17.069973 16-17 CATGGGA 6830 0.0 16.746603 4 TTGCGTT 995 0.0 16.217587 4 TACTTTT 15755 0.0 16.191904 14-15 TACATCG 300 1.4807374E-6 15.820097 5 AGAGTAC 24475 0.0 15.5909 10-11 GTAGTAC 890 0.0 15.464589 3 AGTACAT 8550 0.0 14.98746 2 CCGATCG 195 0.004488504 14.603166 9 TACACTG 2210 0.0 14.603166 5 >>END_MODULE