FastQCFastQC Report
Thu 26 May 2016
SRR938868_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938868_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2900441
Sequences flagged as poor quality0
Sequence length101
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT152550.5259545014016833No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT150680.5195072059731606No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT113730.39211278560743007No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT92550.3190894074383861No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT79120.27278610390626806No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT77330.2666146286030297No Hit
CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC62030.21386402964238888No Hit
GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA56020.1931430427303986No Hit
GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA46600.1606652229781609No Hit
GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT46010.1586310495541885No Hit
TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT41560.14328855508524393No Hit
ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC35860.12363637115873069No Hit
TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC35720.12315368593948299No Hit
ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC35260.12156772021909772No Hit
GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG33090.11408609932075846No Hit
GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT32410.1117416282558411No Hit
GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA31440.1083973092367678No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTTGCGT8350.029.4859833
GTACATG107950.024.9960211
CTTTGCG10150.024.256942
TACATGG109250.023.7496832
ACATGGG108800.023.0210693
GAGTACT110050.021.61852312-13
TTGCGTT11700.021.0434134
AGTACTT115700.020.62420312-13
GTACTTT120900.019.7184114-15
CATGGGA68400.019.1052044
CATGGGG44250.019.0460744
GTATTAC6100.017.1030711
ATGGGAT30100.016.8312365
TACTTTT142550.016.67382414-15
ACTTTTT145450.016.55339816-17
AGAGTAC230650.016.01068510-11
GTATAGA8450.015.7138491
AGTACAT83150.015.6591862
GTATCAA369350.014.8808121
TGGACGC6700.014.8403195
Produced by FastQC (version 0.11.5)