FastQCFastQC Report
Thu 26 May 2016
SRR938868_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938868_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2900441
Sequences flagged as poor quality0
Sequence length101
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT179280.6181129007623324No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT149370.5149906514216287No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT126800.4371748985757683No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT85240.29388634349052434No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT68150.2349642692266452No Hit
GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA55330.19076409414982065No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT55300.190660661602839No Hit
GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT51290.17683517782295866No Hit
CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC49560.17087056761368358No Hit
GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA47560.16397506448157365No Hit
ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC44630.15387315239303265No Hit
TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC44370.15297673698585837No Hit
GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG40400.13928916326862018No Hit
GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA39080.13473813120142766No Hit
TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT37980.13094560447876719No Hit
GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA30130.1038807546852358No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT29020.10005375044691481No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTTGCGT8950.027.5701833
CTTTGCG10350.022.9239272
TTGCGTT11500.022.2820594
GTACATG109450.021.975571
GAGTACT126300.021.717712-13
TACATGG111150.021.1327062
AGTACTT129050.020.6849212-13
ACATGGG108950.020.3834653
GTACTTT135750.019.97863214-15
GTAAGCG3700.019.2375194-95
CATGGGA70350.017.4700874
TACTTTT157700.016.85177614-15
ACTTTTT163750.016.66272216-17
GTGTAGC14300.016.6532331
TAGCCCT18250.016.6408884
GTCGTAT1800.002573197715.87608051
GTATAGC11250.015.66441
AGAGTAC259600.015.10871410-11
AGTACAT85850.015.0344652
ATAGCGG1900.00378125314.9847536
Produced by FastQC (version 0.11.5)