##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938868_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2900441 Sequences flagged as poor quality 0 Sequence length 101 %GC 43 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.865201877921322 31.0 31.0 34.0 30.0 34.0 2 32.118266153319446 33.0 31.0 34.0 30.0 34.0 3 32.202309924594225 34.0 31.0 34.0 30.0 34.0 4 35.853647428098 37.0 35.0 37.0 35.0 37.0 5 35.76053262245293 37.0 35.0 37.0 33.0 37.0 6 35.81456095814395 37.0 35.0 37.0 35.0 37.0 7 35.74817450173956 37.0 35.0 37.0 33.0 37.0 8 35.71864347525083 37.0 35.0 37.0 33.0 37.0 9 37.33675465213738 39.0 37.0 39.0 34.0 39.0 10-11 37.23874197061757 39.0 37.0 39.0 33.0 39.0 12-13 37.15490351294855 39.0 37.0 39.0 33.0 39.0 14-15 38.27048024765889 40.0 38.0 41.0 33.0 41.0 16-17 38.25822038786515 40.0 37.5 41.0 33.0 41.0 18-19 38.40381755739903 40.0 38.0 41.0 33.5 41.0 20-21 38.41000196177064 40.0 38.0 41.0 34.0 41.0 22-23 38.332125183722056 40.0 38.0 41.0 34.0 41.0 24-25 38.24661939339569 40.0 38.0 41.0 33.0 41.0 26-27 38.042087910079886 40.0 37.0 41.0 33.0 41.0 28-29 37.8387217667934 40.0 37.0 41.0 33.0 41.0 30-31 37.64464421100102 40.0 37.0 41.0 32.0 41.0 32-33 37.443723040737595 39.5 36.0 41.0 32.0 41.0 34-35 37.37689561690791 40.0 36.0 41.0 32.0 41.0 36-37 37.23471982364062 39.5 36.0 41.0 31.0 41.0 38-39 37.079382411157475 39.0 36.0 41.0 31.0 41.0 40-41 36.90966787464389 39.0 36.0 41.0 31.0 41.0 42-43 36.71507815535638 39.0 35.0 41.0 30.0 41.0 44-45 36.415415104116924 39.0 35.0 40.5 30.0 41.0 46-47 36.04616539346948 39.0 35.0 40.0 29.5 41.0 48-49 35.69697694247185 39.0 35.0 40.0 28.0 41.0 50-51 35.39317245205126 38.0 35.0 40.0 27.0 41.0 52-53 35.13606758420529 38.0 34.0 40.0 26.5 41.0 54-55 34.87284106106623 38.0 34.0 40.0 26.0 41.0 56-57 34.555249701683294 38.0 34.0 40.0 25.5 41.0 58-59 34.21997482451806 38.0 33.0 40.0 24.5 41.0 60-61 33.74211628507527 37.0 33.0 40.0 23.0 41.0 62-63 33.3828816031769 36.5 32.0 39.0 22.5 40.0 64-65 33.07700673794089 36.0 32.0 39.0 22.0 40.0 66-67 32.54762965355958 35.5 31.0 39.0 20.5 40.0 68-69 32.08401670642499 35.0 31.0 38.0 19.5 40.0 70-71 31.54400830770217 35.0 30.5 37.5 18.0 39.5 72-73 30.953029901315006 34.5 30.0 37.0 14.0 39.0 74-75 30.42837261644005 34.0 29.0 36.5 10.5 39.0 76-77 28.5432437687924 31.5 27.0 34.5 7.5 36.5 78-79 29.534893142111837 34.0 29.0 35.0 7.0 37.0 80-81 29.49247976428412 34.0 29.0 35.0 6.5 37.0 82-83 29.089568620771807 34.0 29.0 35.0 2.0 36.5 84-85 28.588502231212424 33.5 28.5 35.0 2.0 36.0 86-87 27.889584032221308 33.0 26.5 35.0 2.0 36.0 88-89 27.174862891539597 32.5 25.5 35.0 2.0 35.0 90-91 26.545888021855987 32.0 25.0 34.0 2.0 35.0 92-93 26.02607258689282 32.0 24.0 34.0 2.0 35.0 94-95 25.408328423160476 31.5 21.5 34.0 2.0 35.0 96-97 24.474672816995763 31.0 17.0 34.0 2.0 35.0 98-99 23.207479828067527 31.0 2.0 34.0 2.0 35.0 100-101 18.7183457619031 24.0 2.0 31.5 2.0 33.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.15114305532157246 1101 2 0.12155234929487335 1101 3 0.17874811398407076 1101 4 0.0835693638640791 1101 5 0.18507514327308172 1101 6 0.11064158026855608 1101 7 0.05642687315543782 1101 8 0.1515761676076366 1101 9 0.2194195585327634 1101 10-11 0.2901293605296402 1101 12-13 0.24793854672203253 1101 14-15 0.3015414205972107 1101 16-17 0.1753365656043755 1101 18-19 0.280089876798165 1101 20-21 0.2874603678835044 1101 22-23 0.2684931777991295 1101 24-25 0.28003510814082233 1101 26-27 0.2966032264680578 1101 28-29 0.3067536938348283 1101 30-31 0.25610018560062997 1101 32-33 0.3253316663995989 1101 34-35 0.46883611629928623 1101 36-37 0.3794630988265979 1101 38-39 0.46119145618992263 1101 40-41 0.4803967931966966 1101 42-43 0.2850553715130246 1101 44-45 0.401199305349472 1101 46-47 0.4342158212109979 1101 48-49 0.28153423478589445 1101 50-51 0.2926200197337039 1101 52-53 0.489193810812381 1101 54-55 0.599599774720609 1101 56-57 0.33740880780580795 1101 58-59 0.5443508548009675 1101 60-61 0.6490746956920148 1101 62-63 0.6532503383400083 1101 64-65 0.5794504776072671 1101 66-67 0.665107110568961 1101 68-69 0.5395296552611626 1101 70-71 0.6325364601853245 1101 72-73 0.3517065640635266 1101 74-75 0.5062607553373013 1101 76-77 0.4024657729336347 1101 78-79 0.6243892253249754 1101 80-81 0.8043787677276484 1101 82-83 0.6079053328077606 1101 84-85 0.6444055408676448 1101 86-87 0.9387275553515977 1101 88-89 0.9797804395423597 1101 90-91 1.0141846084364587 1101 92-93 0.9855315278364998 1101 94-95 0.7322603160673182 1101 96-97 0.9013857462391357 1101 98-99 0.6932291689095784 1101 100-101 1.064018034999389 1102 1 0.1830481019004857 1102 2 0.20135018716730002 1102 3 0.18384377524830597 1102 4 0.15878348420298494 1102 5 0.07806423323113165 1102 6 0.1683512214159819 1102 7 0.14511340993324495 1102 8 0.24945012144594614 1102 9 0.30323491530177193 1102 10-11 0.33112899642736693 1102 12-13 0.30068980795890354 1102 14-15 0.3069027285527355 1102 16-17 0.36594315695580093 1102 18-19 0.2997766299632545 1102 20-21 0.31757696281524517 1102 22-23 0.3879547667798917 1102 24-25 0.34003148608834266 1102 26-27 0.3742301092808802 1102 28-29 0.3686190205106996 1102 30-31 0.39309464590912313 1102 32-33 0.3488239775688129 1102 34-35 0.4180735060489482 1102 36-37 0.44195343190492764 1102 38-39 0.4337709759872084 1102 40-41 0.5233763537673681 1102 42-43 0.31060038986927196 1102 44-45 0.3862788899617655 1102 46-47 0.36580854164431287 1102 48-49 0.2002783437337925 1102 50-51 0.21020390837885827 1102 52-53 0.26427554412131116 1102 54-55 0.2498575993467469 1102 56-57 0.22143565753451355 1102 58-59 0.1515448277258784 1102 60-61 0.10681246339223804 1102 62-63 0.2727348180854108 1102 64-65 0.23530648533903076 1102 66-67 0.4395902693528342 1102 68-69 0.48313952125660364 1102 70-71 0.4449207179661947 1102 72-73 0.5839665060221613 1102 74-75 0.48733812371888163 1102 76-77 0.4120782499938649 1102 78-79 0.4245830127208663 1102 80-81 0.5881711957915208 1102 82-83 0.775927592242585 1102 84-85 0.8316193667883098 1102 86-87 0.8762609983160949 1102 88-89 1.096556220961613 1102 90-91 1.1391660966371546 1102 92-93 1.2447908737499702 1102 94-95 1.2519712879143334 1102 96-97 0.9073836956819115 1102 98-99 1.0398485887007816 1102 100-101 1.3466188153847582 1103 1 0.0902013450106054 1103 2 0.15071949946249674 1103 3 0.16517407618697888 1103 4 0.11765023945561381 1103 5 0.04706924822607306 1103 6 0.09220719809508182 1103 7 0.15171623719778893 1103 8 0.17289427022660675 1103 9 0.17672654060855564 1103 10-11 0.29969797684586297 1103 12-13 0.17698280609241124 1103 14-15 0.30731820317149783 1103 16-17 0.25958253479512905 1103 18-19 0.25374162723260696 1103 20-21 0.2841215104590802 1103 22-23 0.2372985646077268 1103 24-25 0.29764094019819964 1103 26-27 0.22705966874071493 1103 28-29 0.30836872471766696 1103 30-31 0.3869158441937586 1103 32-33 0.33855129217049296 1103 34-35 0.27256766758684847 1103 36-37 0.13077916406891887 1103 38-39 0.3384365899672801 1103 40-41 0.3160177374286306 1103 42-43 0.37654076398001735 1103 44-45 0.42122679404699426 1103 46-47 0.5677245536513311 1103 48-49 0.42876297676873065 1103 50-51 0.38142384069823976 1103 52-53 0.5836421691608749 1103 54-55 0.5191336285361032 1103 56-57 0.47264168453206423 1103 58-59 0.30509973243267297 1103 60-61 0.47687887519926164 1103 62-63 0.6944343884388857 1103 64-65 0.5192381176324261 1103 66-67 0.7078541450293514 1103 68-69 0.7591926657920993 1103 70-71 0.7209209221825326 1103 72-73 0.7942895916103083 1103 74-75 0.7313122013218454 1103 76-77 0.6350027034039307 1103 78-79 0.7028270188015817 1103 80-81 0.8106294977452464 1103 82-83 0.9311543295935856 1103 84-85 0.7830126717367492 1103 86-87 0.8115029160835583 1103 88-89 1.0538650721428517 1103 90-91 1.1810309489305197 1103 92-93 1.0844273535783913 1103 94-95 1.0954617223783032 1103 96-97 0.9672620260020857 1103 98-99 1.316943940754534 1103 100-101 1.3703173417702281 1104 1 0.05476029161687279 1104 2 0.015065619909599093 1104 3 0.07981164836822785 1104 4 0.14183767054964136 1104 5 0.04211974957429021 1104 6 0.09509717466910672 1104 7 -0.028074244256558245 1104 8 0.06764392839971123 1104 9 0.10189876035838097 1104 10-11 0.059940703425318986 1104 12-13 0.1234382124741984 1104 14-15 0.11889114714664117 1104 16-17 0.11588917593262948 1104 18-19 0.16336882443980727 1104 20-21 0.08008538492753559 1104 22-23 -0.0048412558756041335 1104 24-25 0.11657522661800357 1104 26-27 0.020609733618385917 1104 28-29 -3.2372628773202905E-5 1104 30-31 6.798031027486218E-4 1104 32-33 0.10802255214168355 1104 34-35 0.03818995866976849 1104 36-37 0.09538605077896989 1104 38-39 0.22276707534560813 1104 40-41 0.19877019439065435 1104 42-43 0.17497444157318398 1104 44-45 0.25851789155703386 1104 46-47 0.2947304570058549 1104 48-49 0.2621114446321826 1104 50-51 0.14609912449287776 1104 52-53 0.16744254859792562 1104 54-55 0.2792486416644664 1104 56-57 0.25233166932439843 1104 58-59 0.27992255379908926 1104 60-61 0.23259411554849407 1104 62-63 0.1931206647217607 1104 64-65 0.17770983785398187 1104 66-67 0.3467313624335375 1104 68-69 0.3462428262329098 1104 70-71 0.23489895014226647 1104 72-73 0.2882459044785861 1104 74-75 0.2969853875467017 1104 76-77 0.25859666645124335 1104 78-79 0.4194051252156754 1104 80-81 0.5324045396960067 1104 82-83 0.706299833515736 1104 84-85 0.6785299422859623 1104 86-87 0.5175650183027649 1104 88-89 0.6804672132899334 1104 90-91 0.8798872603910368 1104 92-93 1.034545168285831 1104 94-95 0.9992758649372107 1104 96-97 1.0203622858805694 1104 98-99 1.0419193651100578 1104 100-101 1.785560126055362 1105 1 -0.02933160972009219 1105 2 0.007098623222937306 1105 3 0.033284693231365736 1105 4 0.00359009937983501 1105 5 0.05865405181163652 1105 6 0.06577936107948545 1105 7 0.031518575591157116 1105 8 0.059786078779239915 1105 9 -0.013756600019114273 1105 10-11 -0.04727060381652848 1105 12-13 0.03891637517675406 1105 14-15 0.06406427213308774 1105 16-17 0.03810864826200344 1105 18-19 0.07108079149168844 1105 20-21 0.08079970689511384 1105 22-23 -0.025129367173093442 1105 24-25 0.07062979374516942 1105 26-27 0.027849411705993532 1105 28-29 0.041490509819588794 1105 30-31 0.013070150954199278 1105 32-33 -0.06112823687087854 1105 34-35 0.11345991903188946 1105 36-37 0.21590252089878703 1105 38-39 0.08687916273027696 1105 40-41 0.23261040456404203 1105 42-43 0.1680187799202173 1105 44-45 0.17003768684345744 1105 46-47 0.27446846216213316 1105 48-49 0.289806219173272 1105 50-51 0.3028865444434814 1105 52-53 0.44142619969786523 1105 54-55 0.3184948961707832 1105 56-57 0.5091024831525175 1105 58-59 0.4777325166841635 1105 60-61 0.42220363514371684 1105 62-63 0.3947844060170951 1105 64-65 0.2656370389782694 1105 66-67 0.4782220403378332 1105 68-69 0.35533794285026943 1105 70-71 0.4390675303729985 1105 72-73 0.5188254261237084 1105 74-75 0.6561636826054666 1105 76-77 0.4212419330657404 1105 78-79 0.5723650064005952 1105 80-81 0.5252275041401795 1105 82-83 0.7599068730333798 1105 84-85 0.5933822340294199 1105 86-87 0.5708730121057748 1105 88-89 0.7932862898318369 1105 90-91 0.9523112664382225 1105 92-93 0.9505199131822799 1105 94-95 1.0653354636702161 1105 96-97 0.8100892925604697 1105 98-99 1.07107696903876 1105 100-101 1.0865356248796267 1106 1 0.03574729639548835 1106 2 -0.07567107224056002 1106 3 -0.11085159306735903 1106 4 -0.037865242913078134 1106 5 -0.04517105476658401 1106 6 -5.521975433140369E-4 1106 7 0.02622694317609131 1106 8 -0.05277055583430723 1106 9 -0.07880444073486359 1106 10-11 -0.004296760883192974 1106 12-13 0.05456353914470924 1106 14-15 -0.024501868492805556 1106 16-17 -0.10486447777748964 1106 18-19 -0.08050569458145418 1106 20-21 -0.08233797017765454 1106 22-23 0.1350418031800018 1106 24-25 0.17717706572513237 1106 26-27 0.13597994373680677 1106 28-29 0.10561845625328203 1106 30-31 0.12500272947796987 1106 32-33 0.11527269317939925 1106 34-35 0.06639034209484151 1106 36-37 0.1265553244316422 1106 38-39 0.1979717806354202 1106 40-41 0.35022723436389214 1106 42-43 0.2930549830592568 1106 44-45 0.17818614122032983 1106 46-47 0.2850286669070101 1106 48-49 0.21454383602696936 1106 50-51 0.37546774461935684 1106 52-53 0.3135999652066701 1106 54-55 0.2549162099026532 1106 56-57 0.3144662828810496 1106 58-59 0.3774878169193059 1106 60-61 0.4253295312610206 1106 62-63 0.42755003908214917 1106 64-65 0.29771182534390306 1106 66-67 0.4831652623455156 1106 68-69 0.4363683343698881 1106 70-71 0.4876547715743236 1106 72-73 0.6497537797531017 1106 74-75 0.7421492443634818 1106 76-77 0.41272212767852423 1106 78-79 0.5444266975053758 1106 80-81 0.6716527129952539 1106 82-83 0.7391844591084329 1106 84-85 0.5200407679337182 1106 86-87 0.468039608676154 1106 88-89 0.7387381958135997 1106 90-91 1.10371757319232 1106 92-93 1.0229782855465004 1106 94-95 1.1762537568893556 1106 96-97 0.8618058948659986 1106 98-99 0.6601387222743433 1106 100-101 1.1282839377894511 1107 1 -0.024466359036480867 1107 2 -0.013377908174987851 1107 3 -0.09666510443479837 1107 4 -0.10937974544930995 1107 5 -0.08343469043802543 1107 6 -0.09518470609110352 1107 7 -0.11591785376181463 1107 8 -0.07681458736897184 1107 9 0.00931869576265143 1107 10-11 -0.05685173080730266 1107 12-13 -0.043615349842212936 1107 14-15 -0.008677189707150035 1107 16-17 0.03808844623693375 1107 18-19 -0.07909985682567111 1107 20-21 -0.06583786805138203 1107 22-23 -0.0017550211156560636 1107 24-25 0.015292346354861763 1107 26-27 -0.10077971626123627 1107 28-29 -0.0017111288239490818 1107 30-31 -0.03411170876101011 1107 32-33 0.04475467725737303 1107 34-35 -0.09389981740344666 1107 36-37 0.019544264578392756 1107 38-39 -0.030893396961580777 1107 40-41 -0.06235309188426186 1107 42-43 -0.008561573242538145 1107 44-45 0.002213615002879976 1107 46-47 -0.10726243046102724 1107 48-49 -0.037139495151038204 1107 50-51 0.006144727047598053 1107 52-53 0.10583486290514799 1107 54-55 -0.10940703125919526 1107 56-57 0.04936011384936734 1107 58-59 0.12324788421387467 1107 60-61 -0.07423851687006788 1107 62-63 -0.16605746000837485 1107 64-65 0.0204671924123474 1107 66-67 0.11603989357295319 1107 68-69 -0.005218091657440027 1107 70-71 0.00701870008834149 1107 72-73 0.015806278704644683 1107 74-75 -0.14937578503559124 1107 76-77 -0.29223075524366493 1107 78-79 0.08709451394879508 1107 80-81 0.18569858159438013 1107 82-83 0.33521851489959786 1107 84-85 0.3596195363028514 1107 86-87 0.28339940137793107 1107 88-89 0.4213715310403998 1107 90-91 0.5292771224830588 1107 92-93 0.6097849598917762 1107 94-95 0.5820299347558731 1107 96-97 0.5821065449692746 1107 98-99 0.501005913390685 1107 100-101 1.0442161815340647 1108 1 0.04813479104966234 1108 2 -0.04614412927566747 1108 3 0.02962214434565169 1108 4 6.239779570336168E-4 1108 5 0.054989563016548004 1108 6 0.04239957338316458 1108 7 0.012086503457567233 1108 8 0.10813250003443642 1108 9 0.12075671551878031 1108 10-11 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-1.8611018775294106 2316 74-75 -2.1181549660067525 2316 76-77 -2.111872783424335 2316 78-79 -2.246696847167236 2316 80-81 -2.234225364744148 2316 82-83 -2.4371413675478273 2316 84-85 -2.615198313255071 2316 86-87 -2.6668629221350635 2316 88-89 -2.8547513753998963 2316 90-91 -3.1785057590189965 2316 92-93 -3.4234928871467574 2316 94-95 -3.725938339290778 2316 96-97 -4.031442100389068 2316 98-99 -4.412263064435297 2316 100-101 -4.4614750057703105 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 1.0 3 9.0 4 32.0 5 100.0 6 207.0 7 530.0 8 1167.0 9 2172.0 10 3503.0 11 5385.0 12 7824.0 13 10605.0 14 12946.0 15 17383.0 16 22469.0 17 30999.0 18 29761.0 19 21826.0 20 20680.0 21 21971.0 22 24389.0 23 27326.0 24 31259.0 25 37313.0 26 44172.0 27 52966.0 28 63943.0 29 76594.0 30 91374.0 31 110941.0 32 136554.0 33 170861.0 34 225391.0 35 313318.0 36 445994.0 37 552683.0 38 275518.0 39 10275.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 41.55672937345691 21.25592363762823 15.532448298473478 21.65489869044138 2 15.613591174583451 22.69282498764843 39.076333564447616 22.617250273320504 3 19.982581959088293 26.08134418179856 31.060311173369843 22.875762685743307 4 13.244675551062752 18.262084972595545 37.85348503899924 30.63975443734246 5 12.832248493066711 38.50964013229776 34.83080249908031 13.82730887555522 6 29.942067430435575 37.929714826124716 17.34384529800813 14.784372445431574 7 26.328892744241305 34.28182128166027 21.714456525749014 17.674829448349406 8 24.9029716515523 35.400926962486054 20.17569052430303 19.520410861658625 9 25.259503640998044 17.854078052268605 21.748175536065034 35.13824277066832 10-11 24.284962183336948 27.217292129024518 27.754796598172486 20.74294908946605 12-13 25.95232931819678 24.915280124643115 28.005155078141563 21.127235479018537 14-15 22.407529708356197 26.291574158018307 27.504732529548896 23.796163604076604 16-17 20.735985155002584 29.39370515013424 28.22216537125768 21.648144323605496 18-19 20.651600911723424 28.42364316322932 30.415029990266994 20.509725934780263 20-21 21.901376376902686 27.033302866701998 30.07656421902738 20.98875653736794 22-23 21.273489100450586 27.07679625270778 30.11012808052293 21.539586566318707 24-25 21.626142369384517 27.24811502802505 30.25577834543092 20.869964257159516 26-27 21.556877040422474 26.889135134967407 30.815055365718525 20.738932458891597 28-29 20.829194594890915 27.827768260068037 30.49029440695398 20.85274273808707 30-31 21.244648658600536 27.304589198677025 30.193339564569666 21.25742257815277 32-33 21.188570979378653 27.36666596562385 30.266276748949554 21.17848630604794 34-35 21.8490754433152 27.150325666132137 30.10631772643631 20.894281164116354 36-37 21.750449574320225 26.900775745208982 30.572053691353844 20.77672098911695 38-39 21.371802117387677 27.428921861891535 30.52471256857131 20.67456345214947 40-41 21.44180368027511 27.499007888963554 30.188602807700093 20.870585623061245 42-43 21.025486760141394 27.691745440973786 29.752183083355817 21.530584715529002 44-45 21.56535647103604 27.326756841739435 29.52604991256475 21.581836774659774 46-47 21.9043227248397 27.79157555222463 29.060457467045808 21.243644255889865 48-49 21.805033626869914 27.75324791966442 29.148970317712852 21.292748135752813 50-51 21.233592008870453 28.092504661400298 29.418797165647963 21.25510616408129 52-53 21.674824621497212 28.449880552646995 28.59154866449619 21.283746161359602 54-55 21.178727648657567 27.79675573473137 29.051513200923583 21.973003415687476 56-57 21.687219288377182 28.10062331900563 28.609304585061373 21.602852807555816 58-59 22.16566555212082 27.73107403926447 28.64388570813885 21.459374700475863 60-61 21.59632943699473 27.837889788294433 29.374175213700997 21.191605561009837 62-63 21.48268142672097 27.707096955256116 29.33426330685575 21.475958311167165 64-65 21.271457118860752 28.272404562624377 28.969403220757155 21.486735097757716 66-67 21.395462534986724 27.86806652631753 28.587436292962387 22.149034645733362 68-69 21.07589342741725 28.046629599231416 28.883982651295625 21.99349432205571 70-71 21.66751538817718 27.870037694267875 28.593841419287617 21.868605498267332 72-73 21.557566590735686 27.38502524271309 28.98462682054212 22.07278134600911 74-75 21.092533859506194 28.585187562856824 28.35096456021688 21.97131401742011 76-77 21.421328014198473 27.6790833121348 28.57111299721491 22.328475676451816 78-79 21.39605787193902 27.806691988125245 28.730810875665867 22.066439264269867 80-81 21.470924593880724 27.924632840316356 28.558536443251214 22.04590612255171 82-83 21.340651300957337 27.826958038450016 28.77405539364531 22.05833526694734 84-85 21.18395099228014 27.912858768718273 28.54169417685104 22.36149606215055 86-87 21.139568042311968 27.853848331480574 28.598744036070123 22.407839590137336 88-89 21.304252472636513 27.706511170852394 28.283198603874965 22.706037752636128 90-91 21.19688103700729 27.36559895672559 28.607539597925697 22.82998040834142 92-93 21.332428413472297 27.851799088483443 28.56560433396163 22.250168164082634 94-95 21.18520942160175 27.70137368765646 28.371547637066225 22.74186925367556 96-97 20.623701698888954 27.551858779316824 28.744770162993355 23.079669358800867 98-99 20.92692111303074 27.675877564825484 28.06152581624656 23.335675505897207 100-101 21.050269864646186 26.84948518937932 28.90779677741498 23.192448168559515 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 2843.0 1 1911.5 2 1118.0 3 1263.0 4 1115.0 5 1122.0 6 1921.0 7 3756.0 8 5833.5 9 6540.5 10 5338.5 11 4128.0 12 4039.0 13 4202.0 14 4421.5 15 4655.5 16 4855.0 17 5177.5 18 5573.5 19 5988.0 20 6652.5 21 7727.5 22 9200.5 23 11087.5 24 13259.5 25 16372.0 26 21098.5 27 26130.5 28 30218.5 29 34315.0 30 38895.0 31 44102.0 32 50052.5 33 56837.5 34 64251.5 35 70110.0 36 76931.0 37 83534.0 38 85526.5 39 86658.5 40 89035.5 41 91753.0 42 94222.5 43 95547.0 44 97559.0 45 100661.5 46 103151.0 47 116495.0 48 149994.0 49 148253.5 50 114358.0 51 98581.5 52 90295.0 53 85710.0 54 81043.0 55 74033.0 56 65775.5 57 56565.0 58 49432.0 59 43365.5 60 36770.0 61 29687.0 62 23144.5 63 17629.5 64 12832.0 65 9088.5 66 6122.0 67 4312.5 68 2935.0 69 1751.5 70 1102.5 71 722.5 72 436.5 73 266.0 74 183.5 75 122.0 76 71.5 77 36.0 78 19.5 79 8.0 80 8.0 81 8.5 82 3.5 83 2.5 84 1.5 85 1.0 86 2.0 87 1.0 88 0.0 89 0.0 90 0.0 91 0.5 92 0.5 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.36325510499955005 2 0.0 3 0.0 4 0.0 5 4.826852192476937E-4 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0012584293216100586 16-17 0.0024134260962384688 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.002689246221522865 36-37 0.006809309342958536 38-39 0.012342950606476739 40-41 0.002068650939632973 42-43 4.65446461417419E-4 44-45 3.1029764094494595E-4 46-47 4.65446461417419E-4 48-49 2.585813674541216E-4 50-51 8.619378915137387E-4 52-53 0.0 54-55 0.0 56-57 0.0 58-59 2.4134260962384684E-4 60-61 0.0023617098227476443 62-63 0.0 64-65 1.7238757830274776E-4 66-67 0.0018962633613302253 68-69 0.0017755920565183016 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.11129342055225394 78-79 8.619378915137388E-5 80-81 0.0 82-83 0.0 84-85 0.0 86-87 0.13311768796538181 88-89 0.4094032597111956 90-91 0.31579680469280363 92-93 0.0 94-95 0.0 96-97 5.516402505687929E-4 98-99 0.0 100-101 1.8962633613302252E-4 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2900441.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 47.88120110507014 #Duplication Level Percentage of deduplicated Percentage of total 1 71.12981180479252 34.05780823591063 2 15.06107396228995 14.422846224934815 3 5.670912953600173 8.145903707420315 4 2.7265788565399616 5.222074822352884 5 1.4960569355157334 3.5816501497031896 6 0.9169345750802508 2.6342357273765717 7 0.6134055384233361 2.0559415760948117 8 0.44939183648817893 1.7213936718293834 9 0.3147822767672315 1.3564938148383294 >10 1.4600688885577608 12.250665134864978 >50 0.09844301914230015 3.2480422105077573 >100 0.05655684810179732 4.887752628304557 >500 0.002883134795661474 0.9376907476697673 >1k 0.0025227429461031855 2.8174552655570446 >5k 3.6039184944331224E-4 1.0881975327153028 >10k+ 2.1623510966598732E-4 1.5718485499197128 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 17928 0.6181129007623324 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 14937 0.5149906514216287 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 12680 0.4371748985757683 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 8524 0.29388634349052434 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 6815 0.2349642692266452 No Hit GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 5533 0.19076409414982065 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 5530 0.190660661602839 No Hit GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT 5129 0.17683517782295866 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 4956 0.17087056761368358 No Hit GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA 4756 0.16397506448157365 No Hit ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC 4463 0.15387315239303265 No Hit TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC 4437 0.15297673698585837 No Hit GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG 4040 0.13928916326862018 No Hit GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA 3908 0.13473813120142766 No Hit TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT 3798 0.13094560447876719 No Hit GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA 3013 0.1038807546852358 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2902 0.10005375044691481 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 3.447751566054955E-5 0.0 5 0.0 0.0 0.0 3.447751566054955E-5 0.0 6 0.0 0.0 0.0 3.447751566054955E-5 0.0 7 0.0 0.0 0.0 3.447751566054955E-5 3.447751566054955E-5 8 0.0 0.0 0.0 3.447751566054955E-5 6.89550313210991E-5 9 0.0 0.0 0.0 2.758201252843964E-4 1.0343254698164865E-4 10-11 0.0 0.0 0.0 3.2753639877522076E-4 1.0343254698164865E-4 12-13 0.0 0.0 0.0 3.792526722660451E-4 1.0343254698164865E-4 14-15 0.0 0.0 0.0 4.137301879265946E-4 1.3791006264219822E-4 16-17 0.0 0.0 0.0 6.205952818898919E-4 2.068650939632973E-4 18-19 0.0 0.0 0.0 7.585053445320902E-4 2.068650939632973E-4 20-21 0.0 0.0 0.0 9.13654165004563E-4 2.068650939632973E-4 22-23 0.0 0.0 0.0 0.001223951805949509 2.7582012528439644E-4 24-25 0.0 0.0 0.0 0.0015342494468944552 3.620139144357703E-4 26-27 0.0 0.0 0.0 0.001999695908311874 3.792526722660451E-4 28-29 0.0 0.0 0.0 0.00458550958285309 3.792526722660451E-4 30-31 0.0 0.0 0.0 0.01191198166071987 3.792526722660451E-4 32-33 0.0 0.0 0.0 0.025737465440600238 3.792526722660451E-4 34-35 0.0 0.0 0.0 0.04671703372004464 4.137301879265946E-4 36-37 0.0 0.0 0.0 0.07881560080001627 4.137301879265946E-4 38-39 0.0 0.0 0.0 0.12660143750553796 4.137301879265946E-4 40-41 0.0 0.0 0.0 0.17849009857466502 4.137301879265946E-4 42-43 0.0 0.0 0.0 0.23224054548946177 4.137301879265946E-4 44-45 0.0 0.0 0.0 0.301454158178015 4.137301879265946E-4 46-47 0.0 0.0 0.0 0.38799272248599437 4.137301879265946E-4 48-49 0.0 0.0 0.0 0.47918575140814795 4.137301879265946E-4 50-51 0.0 0.0 0.0 0.5776707748925077 4.137301879265946E-4 52-53 0.0 0.0 0.0 0.6807757854753811 4.137301879265946E-4 54-55 0.0 0.0 0.0 0.7983785913935157 4.137301879265946E-4 56-57 0.0 0.0 0.0 0.944770122888209 4.137301879265946E-4 58-59 0.0 0.0 0.0 1.1060559411482598 4.137301879265946E-4 60-61 0.0 0.0 0.0 1.2879248362576587 4.4820770358714417E-4 62-63 0.0 0.0 0.0 1.4782062451882316 4.4820770358714417E-4 64-65 0.0 0.0 0.0 1.6745729356328918 4.4820770358714417E-4 66-67 0.0 0.0 0.0 1.90534818670678 4.4820770358714417E-4 68-69 0.0 0.0 0.0 2.1655672361547778 4.4820770358714417E-4 70-71 0.0 0.0 0.0 2.4554714265865085 4.8268521924769373E-4 72-73 0.0 0.0 0.0 2.752340075181671 4.8268521924769373E-4 74-75 0.0 0.0 0.0 3.0787559546979235 4.8268521924769373E-4 76-77 1.7238757830274774E-5 0.0 0.0 3.4226691734119052 4.8268521924769373E-4 78-79 3.447751566054955E-5 0.0 0.0 3.8243149920994775 4.8268521924769373E-4 80-81 3.447751566054955E-5 0.0 0.0 4.267471739642351 4.999239770779684E-4 82-83 3.447751566054955E-5 0.0 0.0 4.701836720691785 5.171627349082432E-4 84-85 3.447751566054955E-5 0.0 0.0 5.171937646723377 5.171627349082432E-4 86-87 3.447751566054955E-5 0.0 0.0 5.667638128132928 5.171627349082432E-4 88-89 3.447751566054955E-5 0.0 0.0 6.1997641048378505 5.516402505687928E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TTTGCGT 895 0.0 27.570183 3 CTTTGCG 1035 0.0 22.923927 2 TTGCGTT 1150 0.0 22.282059 4 GTACATG 10945 0.0 21.97557 1 GAGTACT 12630 0.0 21.7177 12-13 TACATGG 11115 0.0 21.132706 2 AGTACTT 12905 0.0 20.68492 12-13 ACATGGG 10895 0.0 20.383465 3 GTACTTT 13575 0.0 19.978632 14-15 GTAAGCG 370 0.0 19.23751 94-95 CATGGGA 7035 0.0 17.470087 4 TACTTTT 15770 0.0 16.851776 14-15 ACTTTTT 16375 0.0 16.662722 16-17 GTGTAGC 1430 0.0 16.653233 1 TAGCCCT 1825 0.0 16.640888 4 GTCGTAT 180 0.0025731977 15.8760805 1 GTATAGC 1125 0.0 15.6644 1 AGAGTAC 25960 0.0 15.108714 10-11 AGTACAT 8585 0.0 15.034465 2 ATAGCGG 190 0.003781253 14.984753 6 >>END_MODULE