FastQCFastQC Report
Thu 26 May 2016
SRR938867_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938867_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4140731
Sequences flagged as poor quality0
Sequence length101
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT151550.36599817761646436No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT123110.29731465289582926No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT78220.18890384330689436No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT42780.10331509098272745No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA137400.031.844651
GTATCAA203700.030.2533741
GTACATG165150.026.838271
TACATGG171450.025.478162
TATCAAC247000.024.9164892
ATCAACG246300.024.8911273
TCAACGC248600.024.7370684
ACATGGG169250.024.4656893
CAACGCA251300.024.3770265
AACGCAG255600.024.0427476
CATGGGG91700.022.7847334
ACGCAGA283400.021.619297
GAGTACT143850.021.51076312-13
CGCAGAG288850.021.1455548
GTACTTT154000.020.0472514-15
GCAGAGT302050.019.5790049
AGAGTAC236350.019.29201710-11
ATGGGGA52100.019.2784825
AGTACTT151050.019.23153512-13
GTGTAGC21100.018.4226861
Produced by FastQC (version 0.11.5)