FastQCFastQC Report
Thu 26 May 2016
SRR938867_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938867_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4140731
Sequences flagged as poor quality0
Sequence length101
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT152010.36710909257326785No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT132460.3198952069091182No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT85370.20617132578764474No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT46610.11256466551437415No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA142550.031.5437851
GTATCAA204950.028.6333261
GTACATG162350.023.3837991
ATCAACG250400.023.1942313
TATCAAC253700.023.116972
TCAACGC252250.023.0241244
CAACGCA256350.022.6558825
TACATGG168050.022.164812
AACGCAG262300.022.1238676
ACATGGG162350.021.7739323
GAGTACT154550.020.90944112-13
TAAGGTG16700.020.1731975
GTACTTT162550.019.90956314-15
CATGGGG85250.019.7590854
AGTACTT161350.019.7488312-13
ACGCAGA292050.019.7402177
CGCAGAG297550.019.343448
AGAGTAC247800.019.22599810-11
GCAGAGT314200.018.1071939
GTACACG7350.017.4978791
Produced by FastQC (version 0.11.5)