##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938867_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 4140731 Sequences flagged as poor quality 0 Sequence length 101 %GC 46 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.996877845964878 33.0 31.0 34.0 30.0 34.0 2 32.22297053346378 34.0 31.0 34.0 30.0 34.0 3 32.30721507869021 34.0 31.0 34.0 30.0 34.0 4 35.93990288188245 37.0 35.0 37.0 35.0 37.0 5 35.845175646522314 37.0 35.0 37.0 35.0 37.0 6 35.87609240977016 37.0 35.0 37.0 35.0 37.0 7 35.80959424797216 37.0 35.0 37.0 33.0 37.0 8 35.775477325138965 37.0 35.0 37.0 33.0 37.0 9 37.431717974434946 39.0 37.0 39.0 34.0 39.0 10-11 37.3514342032844 39.0 37.0 39.0 34.0 39.0 12-13 37.27264099020197 39.0 37.0 39.0 33.0 39.0 14-15 38.49802800520005 40.0 38.0 41.0 34.0 41.0 16-17 38.42125037825447 40.0 38.0 41.0 33.0 41.0 18-19 38.43674752115025 40.0 38.0 41.0 33.5 41.0 20-21 38.512894703857846 40.0 38.0 41.0 34.0 41.0 22-23 38.47483041037923 40.0 38.0 41.0 34.0 41.0 24-25 38.39795437085867 40.0 38.0 41.0 34.0 41.0 26-27 38.239251475162234 40.0 38.0 41.0 33.0 41.0 28-29 38.07123053876236 40.0 37.5 41.0 33.0 41.0 30-31 37.84242878853999 40.0 37.0 41.0 33.0 41.0 32-33 37.62167320214716 40.0 37.0 41.0 32.0 41.0 34-35 37.59065114348167 40.0 37.0 41.0 32.0 41.0 36-37 37.50561132804812 40.0 37.0 41.0 32.0 41.0 38-39 37.406698841339846 40.0 36.0 41.0 32.0 41.0 40-41 37.24101613942079 40.0 36.0 41.0 31.0 41.0 42-43 37.061739943985735 40.0 36.0 41.0 31.0 41.0 44-45 36.81392174473541 40.0 36.0 41.0 30.5 41.0 46-47 36.51755438834351 39.0 35.0 41.0 30.0 41.0 48-49 36.23490550340024 39.0 35.0 41.0 30.0 41.0 50-51 35.97941993817034 39.0 35.0 41.0 29.0 41.0 52-53 35.75763120086768 39.0 35.0 40.5 28.0 41.0 54-55 35.49843034961701 38.5 35.0 40.0 28.0 41.0 56-57 35.23317416659039 38.0 34.0 40.0 27.5 41.0 58-59 34.91906912088711 38.0 34.0 40.0 26.5 41.0 60-61 34.565894765924185 37.5 34.0 40.0 26.0 41.0 62-63 34.1923533549994 37.0 33.5 40.0 26.0 41.0 64-65 33.81887618393951 36.0 33.0 39.0 25.5 41.0 66-67 33.4248445503946 36.0 33.0 39.0 24.5 40.0 68-69 32.97914003590187 35.0 32.0 38.5 23.5 40.0 70-71 32.500143815186256 35.0 32.0 38.0 22.5 40.0 72-73 32.14521566361109 35.0 31.5 37.0 22.0 39.5 74-75 31.72414496377572 35.0 31.0 37.0 20.5 39.0 76-77 29.70207712116532 33.0 28.5 35.0 18.0 37.0 78-79 30.77204870347772 34.0 30.5 36.0 19.0 37.5 80-81 30.837660789846044 34.0 31.0 35.5 19.0 37.0 82-83 30.539812414764448 34.0 31.0 35.0 18.0 37.0 84-85 30.15928274500324 34.0 30.0 35.0 14.5 36.0 86-87 29.62479680520179 34.0 30.0 35.0 7.0 36.0 88-89 29.117958640636157 34.0 29.0 35.0 2.0 35.5 90-91 28.708297882668546 34.0 29.0 35.0 2.0 35.0 92-93 28.419647641926026 34.0 29.0 35.0 2.0 35.0 94-95 28.023619017994648 33.5 28.5 35.0 2.0 35.0 96-97 27.485861554397037 33.0 27.0 35.0 2.0 35.0 98-99 26.723990884701276 33.0 25.5 35.0 2.0 35.0 100-101 23.502288001804512 29.5 12.5 33.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.09073883690417262 1101 2 0.16981071618448595 1101 3 0.19001960625073622 1101 4 0.1810026236428044 1101 5 0.12437243033420486 1101 6 0.22186839437307526 1101 7 0.17843544253516086 1101 8 0.11867402069582056 1101 9 0.2464368830342707 1101 10-11 0.25955695998186457 1101 12-13 0.24572027203432611 1101 14-15 0.3785992040154653 1101 16-17 0.34586259837484334 1101 18-19 0.2912163346644121 1101 20-21 0.2966964893809845 1101 22-23 0.29422294326217013 1101 24-25 0.3119407317915588 1101 26-27 0.28989637056456274 1101 28-29 0.31103098084505376 1101 30-31 0.1733938303790481 1101 32-33 0.30935522292888606 1101 34-35 0.1690045580474333 1101 36-37 0.27482573734103966 1101 38-39 0.2575994755399549 1101 40-41 0.309964038298574 1101 42-43 0.1739920275757214 1101 44-45 0.22778829617961804 1101 46-47 0.1783424860538858 1101 48-49 0.09752286823425038 1101 50-51 0.13509493620983903 1101 52-53 0.28801309953640697 1101 54-55 0.20269658908956956 1101 56-57 0.1739045348750068 1101 58-59 0.10606863904917674 1101 60-61 0.17332858767674963 1101 62-63 0.050631634694120464 1101 64-65 0.05316350887509458 1101 66-67 -0.011527833071617977 1101 68-69 0.10484654837943452 1101 70-71 -0.030373609264124468 1101 72-73 0.03019616379983603 1101 74-75 0.12077958292685054 1101 76-77 0.1750035730486914 1101 78-79 0.14445080455459802 1101 80-81 0.14878220488536087 1101 82-83 0.14819300776758837 1101 84-85 0.2443823365337181 1101 86-87 0.36056840207394814 1101 88-89 0.4397412717205178 1101 90-91 0.5755719897052494 1101 92-93 0.6180029517506362 1101 94-95 0.5937754504379633 1101 96-97 0.4867726056481807 1101 98-99 0.5114442615080179 1101 100-101 1.138035994856196 1102 1 0.08908703201266377 1102 2 0.10101698064197251 1102 3 0.1299172580288115 1102 4 0.07013171412226882 1102 5 0.1199354042086469 1102 6 0.12833724665077284 1102 7 0.14035622851945107 1102 8 0.03217519349404796 1102 9 0.18736237417670054 1102 10-11 0.2074019327580885 1102 12-13 0.1085961665757722 1102 14-15 0.19073251254658885 1102 16-17 0.23165121092018381 1102 18-19 0.25038569521984755 1102 20-21 0.26948891107665673 1102 22-23 0.24151629029258004 1102 24-25 0.09120636291006434 1102 26-27 0.20229094022106864 1102 28-29 0.22831253565319543 1102 30-31 0.24871183214760606 1102 32-33 0.2641624108776952 1102 34-35 0.23010430147861882 1102 36-37 0.186415352364925 1102 38-39 0.18432458381222006 1102 40-41 0.16744126243961688 1102 42-43 0.12056728357196533 1102 44-45 0.07064841562125679 1102 46-47 -0.010151243288078149 1102 48-49 0.0642582995888219 1102 50-51 0.10840490854197071 1102 52-53 0.09568173082863751 1102 54-55 0.08579522312457044 1102 56-57 -0.048100109394823676 1102 58-59 -0.0053351828374346155 1102 60-61 -0.009248920628358803 1102 62-63 -0.11438769594784048 1102 64-65 0.08806061042394475 1102 66-67 -0.026408880216486352 1102 68-69 -0.05634408063032481 1102 70-71 -0.0392987741792723 1102 72-73 0.03694387336594929 1102 74-75 0.12327991390229798 1102 76-77 0.016081731853223147 1102 78-79 0.03860513575237334 1102 80-81 -0.004284303583421689 1102 82-83 0.08891739990394498 1102 84-85 0.08990869363080733 1102 86-87 0.040390295387570774 1102 88-89 0.11586378123501362 1102 90-91 0.20311791708727966 1102 92-93 0.30860961717718993 1102 94-95 0.3438244683206939 1102 96-97 0.4621731697308924 1102 98-99 0.6041793923313037 1102 100-101 1.0106062580110056 1103 1 0.056339483778238275 1103 2 0.12822083626178227 1103 3 0.037612279493266954 1103 4 0.05991154449861824 1103 5 0.046248682793873286 1103 6 0.023144485705252293 1103 7 0.09453666694896867 1103 8 0.09849577571479529 1103 9 0.036134660014170095 1103 10-11 0.007988811215611236 1103 12-13 0.08982739041944399 1103 14-15 0.09629530249162599 1103 16-17 0.11230401161293457 1103 18-19 0.06668404581549225 1103 20-21 0.07973215056701832 1103 22-23 -0.018480105268530167 1103 24-25 0.0300018789735077 1103 26-27 0.14817934693881085 1103 28-29 0.08519244004991577 1103 30-31 0.0190129503983556 1103 32-33 0.11606170234486513 1103 34-35 0.04256204772393346 1103 36-37 0.07018486289378956 1103 38-39 0.12130138893818554 1103 40-41 0.09485874210952971 1103 42-43 0.1704629771603976 1103 44-45 0.19957325414129912 1103 46-47 0.28115037827207345 1103 48-49 0.24963597663182924 1103 50-51 0.21993814719044735 1103 52-53 0.2574586244437498 1103 54-55 0.14153564739873303 1103 56-57 0.09227574720610221 1103 58-59 -0.08934812966120376 1103 60-61 0.10876501896503754 1103 62-63 0.12976556887564783 1103 64-65 0.24361319568861006 1103 66-67 -0.05094302744596746 1103 68-69 0.08251975212370866 1103 70-71 0.00428477791690085 1103 72-73 0.1213876945959882 1103 74-75 0.09364287980518071 1103 76-77 0.10218554479560282 1103 78-79 0.05333167400771188 1103 80-81 0.10352623121836402 1103 82-83 0.12129548404080737 1103 84-85 0.046684036498955805 1103 86-87 0.16411457367066262 1103 88-89 0.3572618734700157 1103 90-91 0.48479278941033144 1103 92-93 0.3163202554471489 1103 94-95 0.24486221142442588 1103 96-97 0.26496243688541554 1103 98-99 0.6432358019821578 1103 100-101 1.102534938957664 1104 1 5.763153169482393E-4 1104 2 0.035369929000587774 1104 3 0.0030148142449988313 1104 4 0.014363579789929304 1104 5 -0.005348170428852939 1104 6 0.03686459899846284 1104 7 -0.008979272027254126 1104 8 0.034241023145405336 1104 9 0.02491130817589493 1104 10-11 0.05314714128697062 1104 12-13 0.022649476972397053 1104 14-15 0.08875329511992192 1104 16-17 0.1319692069673053 1104 18-19 0.10356489258779078 1104 20-21 0.08538942202882538 1104 22-23 0.03605018134540927 1104 24-25 0.039573158323158 1104 26-27 0.07611153681522609 1104 28-29 0.084060120703775 1104 30-31 0.061689657455822555 1104 32-33 -0.03329307567152284 1104 34-35 0.004517883845267079 1104 36-37 -0.022120339611696238 1104 38-39 0.14758388522385957 1104 40-41 0.20271653015260682 1104 42-43 0.07378060255895491 1104 44-45 0.12589464180290122 1104 46-47 0.013664938208407307 1104 48-49 0.01036985220970621 1104 50-51 0.07195664204012786 1104 52-53 -0.023123369317765707 1104 54-55 -0.044565713835460485 1104 56-57 -0.009601275307645096 1104 58-59 -0.11586851573470369 1104 60-61 -0.09214101673141073 1104 62-63 -0.07315586189446321 1104 64-65 0.06922286035772629 1104 66-67 -0.043302079548872996 1104 68-69 -0.06543345855941851 1104 70-71 -0.05636612458649637 1104 72-73 -0.1389814166405543 1104 74-75 -0.16580296674725759 1104 76-77 -0.12452468453736287 1104 78-79 -0.1182733683907351 1104 80-81 -0.1396400241873188 1104 82-83 -0.20536630228081876 1104 84-85 -0.020445037453768578 1104 86-87 0.24638839635050047 1104 88-89 0.40702066764272615 1104 90-91 0.4108038390305353 1104 92-93 0.4289695402999847 1104 94-95 0.3820128828863716 1104 96-97 0.4082740934129312 1104 98-99 0.3851662920279111 1104 100-101 0.9646235608332852 1105 1 0.010836822267833668 1105 2 -0.06461017592616969 1105 3 -0.005786241845818552 1105 4 -0.060200030397354 1105 5 0.018728279801685233 1105 6 -0.05479010009933205 1105 7 0.018305887217721306 1105 8 0.004051865529383747 1105 9 0.05390837742331911 1105 10-11 0.024837960357075417 1105 12-13 -0.012547565053971255 1105 14-15 0.010175027937712855 1105 16-17 0.004866981843420604 1105 18-19 -0.052876011682784 1105 20-21 0.027829366020959867 1105 22-23 0.026613667996500112 1105 24-25 -0.034620202396418165 1105 26-27 0.02046808741173578 1105 28-29 0.03880987192558649 1105 30-31 -0.04708602702987008 1105 32-33 0.010759787764598627 1105 34-35 0.0517487518055475 1105 36-37 -0.0013360032829723423 1105 38-39 0.06914630206586025 1105 40-41 -0.04071630009394056 1105 42-43 -0.10009344941746434 1105 44-45 -0.007369399935100773 1105 46-47 0.02609244598419025 1105 48-49 0.025332545356214098 1105 50-51 1.2510303411517043E-4 1105 52-53 0.059837559846791066 1105 54-55 0.07443178276100326 1105 56-57 -0.02727350505337256 1105 58-59 -0.05509840402422839 1105 60-61 -0.029411204395096036 1105 62-63 -0.07032521313910678 1105 64-65 -0.010684588048860633 1105 66-67 -0.014619686437313817 1105 68-69 -0.08201828249699616 1105 70-71 -0.006451217323039771 1105 72-73 -0.24330055085293623 1105 74-75 -0.20743798624191356 1105 76-77 -0.19355852375859683 1105 78-79 -0.23383456567193406 1105 80-81 -0.04283514062916183 1105 82-83 -0.09056562469915619 1105 84-85 -0.051747547070018385 1105 86-87 -0.005190011964636199 1105 88-89 0.24935481581218255 1105 90-91 0.29769971920617166 1105 92-93 0.2508962452700523 1105 94-95 0.13982358449654342 1105 96-97 0.17697375645359514 1105 98-99 0.2072575622924262 1105 100-101 0.6093807780943088 1106 1 0.009614029408943736 1106 2 -0.047001958758158935 1106 3 -0.10385422912877829 1106 4 -0.08196574328559336 1106 5 -0.030917110269236048 1106 6 -0.10272358016780458 1106 7 -0.07927298292167961 1106 8 -0.10404302319646774 1106 9 -0.09820705422256282 1106 10-11 -0.10123198339444883 1106 12-13 -0.027832475790084743 1106 14-15 0.09577052805999386 1106 16-17 -0.10469525831309312 1106 18-19 -0.18004646900732268 1106 20-21 -0.09041470343367308 1106 22-23 -0.12195566435860172 1106 24-25 -0.13953582968915867 1106 26-27 -0.13641623638381617 1106 28-29 -0.16429602193599635 1106 30-31 -0.10260082282345451 1106 32-33 -0.07300152306934393 1106 34-35 -0.12445569916046395 1106 36-37 -0.03178354546933093 1106 38-39 -0.0799121474328004 1106 40-41 -0.038515272947940105 1106 42-43 -0.01144096714795495 1106 44-45 0.027969313686810438 1106 46-47 0.010440697390400544 1106 48-49 -0.03164960186804677 1106 50-51 -0.05074307989569604 1106 52-53 -0.074669654631073 1106 54-55 -0.0043160773514898665 1106 56-57 -0.00832021574058217 1106 58-59 -0.08126617120446866 1106 60-61 0.005316112797366657 1106 62-63 -0.12596228821859512 1106 64-65 -0.1379773246592748 1106 66-67 -0.2270188060264573 1106 68-69 -0.16761378261927717 1106 70-71 -0.20833431832572558 1106 72-73 -0.3032174009385322 1106 74-75 -0.058257257457370315 1106 76-77 -0.12447072723132635 1106 78-79 -0.16890426486489218 1106 80-81 -0.15264193935745496 1106 82-83 0.07903574482884679 1106 84-85 -0.09356706284404837 1106 86-87 0.11672243606666655 1106 88-89 0.2284450579251711 1106 90-91 0.21650627337589512 1106 92-93 0.3221850689433232 1106 94-95 0.30318871044420703 1106 96-97 0.17342765716281505 1106 98-99 0.31033900029682826 1106 100-101 0.6881286382068055 1107 1 -0.09118286283597499 1107 2 -0.03142465901685654 1107 3 -0.08262088462220873 1107 4 -0.09220015613319532 1107 5 -7.757242171351209E-4 1107 6 -0.0605124988815362 1107 7 -0.06211949461773969 1107 8 -0.04673696437750152 1107 9 -0.0812207804047631 1107 10-11 -0.07615790530905997 1107 12-13 -0.07381483460271454 1107 14-15 -0.09412069448529081 1107 16-17 0.05262532984938417 1107 18-19 0.07244598699001159 1107 20-21 -0.02125813034744084 1107 22-23 -0.10795021033020191 1107 24-25 -0.05050194476664416 1107 26-27 -0.059700770857794794 1107 28-29 -0.11715580620803934 1107 30-31 -0.12692595136147133 1107 32-33 -0.043560901612863745 1107 34-35 -0.13128528827783725 1107 36-37 -0.04995967148311564 1107 38-39 -0.04644508387102064 1107 40-41 -0.08737227330558284 1107 42-43 -0.10544649917039095 1107 44-45 -0.12296444922822047 1107 46-47 0.012517495399613665 1107 48-49 -0.20814750539427251 1107 50-51 -0.11078602033938978 1107 52-53 -0.08923888145788084 1107 54-55 -0.07417991453633732 1107 56-57 -0.1909150075942847 1107 58-59 -0.0795943513182209 1107 60-61 -0.0706288366486092 1107 62-63 -0.11689028193841722 1107 64-65 -0.1645133477705656 1107 66-67 -0.10636383456547804 1107 68-69 -0.04386732255881043 1107 70-71 -0.09060072574494171 1107 72-73 -0.05383442534670735 1107 74-75 -0.05655890778162842 1107 76-77 -0.08983889364649045 1107 78-79 -0.16958012454676563 1107 80-81 -0.1928522372763588 1107 82-83 0.06605662140972512 1107 84-85 -0.13913602372300815 1107 86-87 -0.07597754669783185 1107 88-89 0.09077113787224533 1107 90-91 0.2761025797910648 1107 92-93 0.30546867221578466 1107 94-95 0.3481449454499277 1107 96-97 0.4488060153895397 1107 98-99 0.34764948586819955 1107 100-101 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-0.8976827413711703 2316 52-53 -0.9750831035876644 2316 54-55 -0.9534263064549648 2316 56-57 -0.8690798001674978 2316 58-59 -0.8804931959351379 2316 60-61 -0.9304317164301565 2316 62-63 -0.8718759382426668 2316 64-65 -0.7941568092205742 2316 66-67 -0.8291401310296678 2316 68-69 -0.8429067329032947 2316 70-71 -0.9280605829078468 2316 72-73 -1.0741357467393087 2316 74-75 -0.9813448472444009 2316 76-77 -0.8598296736629152 2316 78-79 -0.9217849190212526 2316 80-81 -0.9842100230771642 2316 82-83 -1.0527042705001826 2316 84-85 -1.1800740636483162 2316 86-87 -1.450944336491336 2316 88-89 -1.7233867519595378 2316 90-91 -2.0492953303736634 2316 92-93 -2.312315606113973 2316 94-95 -2.0893762520021824 2316 96-97 -2.1752836357704517 2316 98-99 -2.5311935058234383 2316 100-101 -3.293037594179303 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 5.0 3 19.0 4 49.0 5 120.0 6 265.0 7 744.0 8 1576.0 9 2900.0 10 4607.0 11 6966.0 12 9886.0 13 13417.0 14 16371.0 15 21348.0 16 26988.0 17 34886.0 18 33717.0 19 24750.0 20 23404.0 21 24549.0 22 25523.0 23 28255.0 24 33730.0 25 40258.0 26 48813.0 27 59657.0 28 73219.0 29 89251.0 30 108622.0 31 132018.0 32 161405.0 33 203627.0 34 270875.0 35 380004.0 36 573494.0 37 877116.0 38 718174.0 39 70109.0 40 14.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.73921351763236 19.239657423754092 14.68873477300178 23.33239428561177 2 16.148066609494798 20.51364843550571 38.96560776346013 24.372677191539367 3 19.67826453831461 24.036697868081745 29.806355447866572 26.47868214573707 4 13.579220673837542 16.844561986760308 35.779165562795555 33.797051776606594 5 14.073577829615108 37.097483511969266 33.73959815307973 15.089340505335894 6 32.84113360660231 35.672421125641826 16.721057223953935 14.765388043801927 7 29.015287397321877 32.53881983640087 20.114419410485734 18.331473355791523 8 26.723397390460768 34.9991583611686 19.124183628446282 19.153260619924357 9 26.559803087908872 16.791865977287586 19.94611579453 36.702215140273545 10-11 25.592594640897946 26.294547991646887 26.576140782871434 21.536716584583736 12-13 26.44267401094155 23.500500273985438 27.369478963980033 22.68734675109298 14-15 22.980028963551398 24.992359423351704 25.791338538464853 26.236273074632045 16-17 22.706058370274125 28.43269208708326 26.319441341726566 22.541808200916044 18-19 22.513796193444843 26.587575051763274 28.808019271019052 22.090609483772834 20-21 23.112259646907756 25.63852855932926 29.024210942464023 22.22500085129896 22-23 22.81288014121178 25.74528507164556 28.99820104227973 22.44363374486292 24-25 22.895691606143938 25.963072703829347 28.87837920405841 22.2628564859683 26-27 22.558145600554106 26.24509566679853 28.764991936196694 22.43176679645067 28-29 22.29217498069785 26.417219568235655 28.948366846337038 22.342238604729456 30-31 22.459560884297964 26.013426131762724 28.903749120626287 22.623263863313024 32-33 22.195151049416154 26.28066155468684 28.863804482831657 22.660382913065348 34-35 22.99473338707987 26.177790573038205 28.45357308989277 22.373902949989155 36-37 22.91104118654585 25.938758222490392 28.420233540103823 22.729967050859937 38-39 22.53390427990133 26.186936614351904 28.723106453002096 22.556052652744672 40-41 22.777482586391784 25.956480470674613 28.797965916665863 22.46807102626774 42-43 22.577571333070566 26.205870874004006 28.361440533877413 22.855117259048015 44-45 22.697777514266157 26.356098362663023 28.175858103660616 22.770266019410208 46-47 22.88315147826675 26.50142730868992 27.77886858616742 22.836552626875907 48-49 23.035055671369868 26.67000826907732 27.52177877899732 22.7731572805555 50-51 22.766547359531152 26.762851378150167 27.604252262321456 22.86634899999722 52-53 23.173033940142453 26.668358799448693 27.404919566134577 22.753687694274273 54-55 22.642270169204423 26.605278150162377 27.547647987758683 23.204803692874517 56-57 22.810030402844326 26.927020856945305 27.382097508869812 22.880851231340554 58-59 23.099400587337467 26.50908537645627 27.3520064143434 23.039507621862864 60-61 22.772256185768928 26.533841748065672 27.85982079419153 22.834081271973865 62-63 22.712680442173134 26.593673918928808 27.816586008605725 22.877059630292333 64-65 22.501087368389786 26.609311254462074 27.60490840868436 23.28469296846378 66-67 22.490429709716693 26.94269466432921 27.295885529885318 23.270990096068786 68-69 22.51685313480322 26.63068803488107 27.648209595554953 23.204249234760756 70-71 22.5239017280489 26.671303617002394 27.440083416804477 23.364711238144228 72-73 22.57768012459636 26.57721547234051 27.551029033279388 23.29407536978374 74-75 22.460007665313203 26.930727931855508 27.206947275734645 23.40231712709664 76-77 22.584311030326997 26.69213719127696 27.348509443691153 23.375042334704883 78-79 22.599369617130527 26.78357728568148 27.33609544092797 23.280957656260025 80-81 22.56858635278957 27.036072633586983 27.236761932500293 23.158579081123154 82-83 22.560014161750665 26.89650692112093 27.195379269988802 23.348099647139602 84-85 22.52558787325233 26.827763020587426 27.04256808761545 23.604081018544793 86-87 22.638275229246847 26.711803529479344 27.226017892289505 23.423903348984297 88-89 22.502162814468257 26.81491360069569 26.95514369435243 23.727779890483628 90-91 22.477587767997083 26.83669741346416 27.130247367393007 23.55546745114575 92-93 22.618494174096313 26.57801243307039 27.19915879587445 23.604334596958847 94-95 22.40583723300897 26.408671033530567 27.211742183562365 23.9737495498981 96-97 22.50756237335417 26.28207752231158 27.225691215595894 23.98466888873835 98-99 22.26966966988724 26.412558777498074 26.96917674854491 24.348594804069773 100-101 22.325649297486947 25.989372343750077 27.539627300271984 24.14535105849099 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 1874.0 1 1271.0 2 848.5 3 1079.5 4 928.5 5 871.5 6 1599.0 7 3425.5 8 5350.5 9 6006.0 10 5085.0 11 3942.0 12 3899.5 13 4282.5 14 4604.5 15 4957.0 16 5287.5 17 5523.5 18 5856.5 19 6306.5 20 6966.5 21 8174.5 22 9822.5 23 11720.0 24 14094.5 25 17287.0 26 21321.0 27 26496.0 28 31303.0 29 36212.0 30 42574.0 31 49825.0 32 58484.0 33 64569.5 34 69923.0 35 75784.5 36 82263.5 37 90364.5 38 96797.0 39 100405.5 40 104354.0 41 114042.0 42 123757.5 43 130287.0 44 136343.0 45 143471.0 46 146810.0 47 152508.5 48 169377.5 49 172540.0 50 163378.5 51 158392.0 52 151322.5 53 151208.0 54 153257.5 55 144009.5 56 131525.0 57 118965.0 58 107077.5 59 94560.5 60 80267.5 61 66775.5 62 55975.5 63 44952.0 64 34433.0 65 27112.5 66 19903.0 67 15703.5 68 11350.5 69 6949.0 70 5393.0 71 4356.0 72 3513.0 73 2925.0 74 2360.5 75 1936.5 76 1306.0 77 715.5 78 453.0 79 284.5 80 152.5 81 101.5 82 71.0 83 40.0 84 20.5 85 17.0 86 11.5 87 4.5 88 4.5 89 4.5 90 1.5 91 1.0 92 0.5 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.3860429474892235 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 2.2942808890507498E-4 16-17 0.0023184312141986524 18-19 1.3282678831346445E-4 20-21 0.0 22-23 0.0 24-25 0.0 26-27 1.2075162573951314E-4 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.007462450470701912 36-37 0.01724333215560248 38-39 0.011398953469810041 40-41 0.007027744618039664 42-43 0.003743300397924907 44-45 0.0017991992235187456 46-47 0.0017146730855010868 48-49 7.245097544370789E-5 50-51 4.2263069008829603E-4 52-53 0.0 54-55 0.0 56-57 0.0 58-59 2.656535766269289E-4 60-61 4.1055552751434465E-4 62-63 0.0 64-65 0.0 66-67 0.0012558169076909366 68-69 7.848855673068353E-4 70-71 0.0018958005241103562 72-73 0.0 74-75 0.0 76-77 0.09790541814959726 78-79 0.03623756288442789 80-81 4.830065029580526E-5 82-83 0.0 84-85 0.0 86-87 0.15138631318962764 88-89 0.32809424229683115 90-91 0.28476855898149384 92-93 0.0 94-95 4.830065029580526E-5 96-97 1.2075162573951314E-5 98-99 2.2942808890507498E-4 100-101 6.037581286975658E-4 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 4140731.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 42.84123896504222 #Duplication Level Percentage of deduplicated Percentage of total 1 67.98137907926176 29.124065063077747 2 15.881942642013982 13.608041999112302 3 6.478156115387647 8.325967025765154 4 3.2113300540328016 5.503094329617649 5 1.7833391010678812 3.8200228292276344 6 1.097841931045432 2.8219745108256453 7 0.6918448203829611 2.0747642493727207 8 0.49645163899036504 1.701488264045848 9 0.3655342499332618 1.40939461361685 >10 1.7699936627205741 13.417221860733077 >50 0.12881123187414245 3.8297332315140244 >100 0.10115680116071267 8.414169878522051 >500 0.0077703998561411145 2.264641085201041 >1k 0.004279350540574727 2.79326634822079 >5k 5.6307243954930416E-5 0.20593566864225113 >10k+ 1.1261448790986083E-4 0.6862190425051093 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 15201 0.36710909257326785 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 13246 0.3198952069091182 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 8537 0.20617132578764474 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 4661 0.11256466551437415 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 1.2075162573951314E-5 0.0 12-13 0.0 0.0 0.0 4.830065029580526E-5 0.0 14-15 0.0 0.0 0.0 4.830065029580526E-5 0.0 16-17 0.0 0.0 0.0 6.0375812869756576E-5 0.0 18-19 0.0 0.0 0.0 1.2075162573951315E-4 0.0 20-21 0.0 0.0 0.0 1.8112743860926973E-4 0.0 22-23 0.0 0.0 0.0 4.347058526622473E-4 0.0 24-25 0.0 0.0 0.0 9.539378433421538E-4 0.0 26-27 0.0 0.0 0.0 0.0014007188585783525 0.0 28-29 0.0 0.0 0.0 0.0038036762107946636 0.0 30-31 0.0 0.0 0.0 0.010565767252207401 0.0 32-33 0.0 0.0 0.0 0.024947285877783415 1.2075162573951314E-5 34-35 0.0 0.0 0.0 0.04716558501385383 2.415032514790263E-5 36-37 0.0 0.0 0.0 0.08526272293467024 2.415032514790263E-5 38-39 0.0 0.0 0.0 0.13863494151153505 2.415032514790263E-5 40-41 0.0 0.0 0.0 0.19589536243721217 2.415032514790263E-5 42-43 0.0 0.0 0.0 0.2561745740063771 2.415032514790263E-5 44-45 0.0 0.0 0.0 0.3340110719580673 2.415032514790263E-5 46-47 0.0 0.0 0.0 0.4314576339298544 2.415032514790263E-5 48-49 0.0 0.0 0.0 0.5336376596306305 2.415032514790263E-5 50-51 0.0 0.0 0.0 0.6415292372288854 2.415032514790263E-5 52-53 0.0 0.0 0.0 0.7501573997441515 2.415032514790263E-5 54-55 0.0 0.0 0.0 0.8734689599493423 2.415032514790263E-5 56-57 0.0 0.0 0.0 1.021594979244003 2.415032514790263E-5 58-59 0.0 0.0 0.0 1.1884495756908624 2.415032514790263E-5 60-61 0.0 0.0 0.0 1.3686472267819378 2.415032514790263E-5 62-63 0.0 0.0 0.0 1.5498108908789292 2.415032514790263E-5 64-65 0.0 0.0 0.0 1.7399826262560887 2.415032514790263E-5 66-67 2.415032514790263E-5 0.0 0.0 1.9651361076099847 2.415032514790263E-5 68-69 2.415032514790263E-5 0.0 0.0 2.21534555130483 2.415032514790263E-5 70-71 2.415032514790263E-5 0.0 0.0 2.4971556954557057 2.415032514790263E-5 72-73 2.415032514790263E-5 0.0 0.0 2.7804631597657514 2.415032514790263E-5 74-75 2.415032514790263E-5 0.0 0.0 3.0714866529605525 2.415032514790263E-5 76-77 2.415032514790263E-5 0.0 0.0 3.3866483961406812 2.415032514790263E-5 78-79 2.415032514790263E-5 0.0 0.0 3.7474542538503464 2.415032514790263E-5 80-81 2.415032514790263E-5 0.0 0.0 4.13868952124637 2.415032514790263E-5 82-83 2.415032514790263E-5 0.0 0.0 4.533668089040317 2.415032514790263E-5 84-85 2.415032514790263E-5 0.0 0.0 4.9335733231644365 2.415032514790263E-5 86-87 2.415032514790263E-5 0.0 0.0 5.35127734692256 2.415032514790263E-5 88-89 2.415032514790263E-5 0.0 0.0 5.803214456577836 2.415032514790263E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 14255 0.0 31.543785 1 GTATCAA 20495 0.0 28.633326 1 GTACATG 16235 0.0 23.383799 1 ATCAACG 25040 0.0 23.194231 3 TATCAAC 25370 0.0 23.11697 2 TCAACGC 25225 0.0 23.024124 4 CAACGCA 25635 0.0 22.655882 5 TACATGG 16805 0.0 22.16481 2 AACGCAG 26230 0.0 22.123867 6 ACATGGG 16235 0.0 21.773932 3 GAGTACT 15455 0.0 20.909441 12-13 TAAGGTG 1670 0.0 20.173197 5 GTACTTT 16255 0.0 19.909563 14-15 CATGGGG 8525 0.0 19.759085 4 AGTACTT 16135 0.0 19.74883 12-13 ACGCAGA 29205 0.0 19.740217 7 CGCAGAG 29755 0.0 19.34344 8 AGAGTAC 24780 0.0 19.225998 10-11 GCAGAGT 31420 0.0 18.107193 9 GTACACG 735 0.0 17.497879 1 >>END_MODULE