FastQCFastQC Report
Thu 26 May 2016
SRR938866_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938866_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4151080
Sequences flagged as poor quality0
Sequence length101
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT153050.3686992300798828No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT123020.296356610809717No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT76660.18467483161008702No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT43250.10418975302812761No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA141450.030.7408711
GTATCAA207750.030.377711
ATCAACG248350.025.0432663
TATCAAC250150.024.9389232
TCAACGC249400.024.8617464
GTACATG170000.024.6277661
CAACGCA252550.024.5328665
AACGCAG258900.023.8747376
ACATGGG166850.023.400553
TACATGG174150.023.3186152
GAGTACT141500.022.27289212-13
ACGCAGA284950.021.542557
CGCAGAG287700.021.3201458
CATGGGG93800.020.9892884
GTACTTT151900.020.71683114-15
GCAGAGT301300.019.8997659
AGTACTT148200.019.87277612-13
AGAGTAC238500.019.19319710-11
TACCTGG31000.019.1293752
ACTTTTT178000.018.0122416-17
Produced by FastQC (version 0.11.5)